pycom06g14090

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0015101
Physical Location & Seq
Reverse (-)
18964415 .. 18969859
5445 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1179 bp
ATGGCTTCCTCTTCTTCTTCATTCCCCACATCTGGGCAAATTACCCACGTTACCCTCACCATCTTTATCTCCTCCCTCCTCTCCTCCTCTATCTCCGCCTCCGCCTCCGCGGTCACAACAAAACAAAACCCTACCTTGCCCTTCAAGAAAATCTACGCCTTTGGTGACTCATTCACAGACACAGGCAACACAAGGTCCATCTCAGGCCCTAGCGGCTTCGGCCACGTCTCAAACGCTCCCTACGGCATCACCTACTTTCACCGCCCCACAAACCGCTACTCCGACGGACGCCTAGTCATTGACTTCGTCGCCGAGTCACTCTCCTTGCCCTACTTGCAACCCTACCGCGCCGTTTCGAGCAATGCCACAGCAGACTCAACTCACGGAGTTAACTTCGCCGTCGCCGGCTCCACCGCCATAGAGCACGAGTTCTTTGTGAAGAACAACCTCAGCCTTGACATTACTCCTCAGTCTATCCAGACTCAGCTGCTTTGGTTCAACAAGTTATTGGAAAGTAAAGGTTGCAAAGTTGGAGGACCAAGTTGCAAATTTGATGATGCACTTTTTTGGGTTGGAGAAATTGGGGTCAATGATTATGCTTATACACTTGGATCTGATGTGCCTGGTGATACAATTCAGAAGCTTGGGGTCAGTAGAGTTACTAGTTGTCTCCAGGCATTGTTAAAGAAGGGTGCGAAATACGTTGTCGTCCAAGGTCTGCCACTTTCAGGGTGCTTGCCGTTGGCAATGACGTTGGCGCCGGAAGACGACAGAGACCGCATCGGATGTGTCAAGAGCGTCAACAACCAAACCTACAGCCACAACCTTGAGCTCCAAGCAAAGTTGCACGAACTCCGGACACAGTTTCCCCATGCTGTCATAGCCTATGCTGACTACTGGAATGCATACTTCACAGTAATGAAGAGTCCGAGCCAGTATGGTTTCAAGGAGAGATTCAAGGCCTGCTGCGGGAGCGGTGACCCCTACAACTTTGACGTGTCTGCCACATGCGGCACGCCTTCTGCCACCGTCTGTAAAAGCCCGTCTCAGTACATAAATTGGGATGGAGTTCATCTCACTGAAGCCATGTACAAGGTGCTTTCTGATATGTTCCTAAAGGGCAATGCCACTCACCCTCCTTTCAGTTACTTGTTGGACAGGAAACTGCGTAACGGATGA

Protein Analysis

393

Amino Acids

42.71

Weight (kDa)

8.26

Isoelectric Point (pI)

34.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 51 - 369 6.9e-26 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015101)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48460
fragaria_vesca FvH4_5g07260
malus_domestica MD06G1157800.v1.1
prunus_persica Prupe.5G157800_v2.0.a1 Prupe.I001600_v2.0.a1
pyrus_communis pycom06g14090 pycom14g13710
rosa_chinensis RchiOBHm_Chr7g0191371
rosa_laevigata RLG00000004461
rosa_multiflora Rmu_co8465791.1_g000001
rosa_roxburghii Rroxscaffold_157G00438320
rosa_rugosa Rorug06G0510300
rosa_samantha Rh7AG117600 Rh7BG119700 Rh7CG122700 Rh7DG120800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 757
AccBSI CCGCTC 1 cut(s) 975
AccII CGCG 2 cut(s) 110, 348
AccIII TCCGGA 1 cut(s) 855
AclWI GGATC 1 cut(s) 619
AcoI YGGCCR 1 cut(s) 220
AcsI RAATTY 1 cut(s) 548
AcuI CTGAAG 1 cut(s) 1101
AcyI GRCGYC 2 cut(s) 289, 758
AfaI GTAC 2 cut(s) 1052, 1091
AfiI CCNNNNNNNGG 4 cut(s) 32, 33, 728, 969
AflIII ACRYGT 1 cut(s) 996
AgsI TTSAA 4 cut(s) 145, 499, 946, 958
AhdI GACNNNNNGTC 1 cut(s) 293
AhlI ACTAGT 1 cut(s) 662
AjiI CACGTC 2 cut(s) 226, 997
AjnI CCWGG 2 cut(s) 622, 672
AluBI AGCT 3 cut(s) 487, 643, 832
AluI AGCT 3 cut(s) 487, 643, 832
Alw21I GWGCWC 2 cut(s) 426, 834
Alw26I GTCTC 4 cut(s) 232, 674, 768, 1050
AlwI GGATC 1 cut(s) 619
Aor13HI TCCGGA 1 cut(s) 855
AoxI GGCC 3 cut(s) 205, 220, 960
ApeKI GCWGC 2 cut(s) 487, 966
ApoI RAATTY 1 cut(s) 548
AspLEI GCGC 2 cut(s) 350, 760
AspS9I GGNCC 3 cut(s) 195, 206, 536
AsuHPI GGTGA 7 cut(s) 49, 176, 241, 251, 638, 989, 1124
AvaII GGWCC 2 cut(s) 195, 536
BanI GGYRCC 1 cut(s) 757
BanII GRGCYC 1 cut(s) 834
BauI CACGAG 1 cut(s) 425
BbsI GAAGAC 1 cut(s) 771
Bbv12I GWGCWC 2 cut(s) 426, 834
BbvCI CCTCAGC 1 cut(s) 449
BbvI GCAGC 2 cut(s) 474, 953
BccI CCATC 3 cut(s) 68, 206, 1058
BceAI ACGGC 4 cut(s) 259, 335, 383, 724
BciT130I CCWGG 2 cut(s) 624, 674
BcoDI GTCTC 4 cut(s) 232, 674, 768, 1050
BcuI ACTAGT 1 cut(s) 662
BfaI CTAG 3 cut(s) 210, 293, 663
BfmI CTRYAG 1 cut(s) 814
BfoI RGCGCY 1 cut(s) 761
BglI GCCNNNNNGGC 1 cut(s) 213
BisI GCNGC 4 cut(s) 214, 488, 967, 1012
BlsI GCNGC 4 cut(s) 215, 489, 968, 1013
Bme1390I CCNGG 2 cut(s) 624, 674
Bme18I GGWCC 2 cut(s) 195, 536
BmeRI GACNNNNNGTC 1 cut(s) 293
BmgBI CACGTC 2 cut(s) 226, 997
BmgT120I GGNCC 3 cut(s) 195, 206, 536
BmiI GGNNCC 2 cut(s) 409, 759
BmrFI CCNGG 2 cut(s) 624, 674
BmsI GCATC 3 cut(s) 255, 547, 789
BpiI GAAGAC 1 cut(s) 771
BplI GAGNNNNNCTC 4 cut(s) 305, 337, 1059, 1091
BpmI CTGGAG 1 cut(s) 656
Bpu10I CCTNAGC 1 cut(s) 449
BpuEI CTTGAG 1 cut(s) 848
BsaHI GRCGYC 2 cut(s) 289, 758
BsaI GGTCTC 1 cut(s) 768
BsaJI CCNNGG 2 cut(s) 108, 712
BsaWI WCCGGW 1 cut(s) 855
BsaXI ACNNNNNCTCC 4 cut(s) 263, 293, 1120, 1150
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 33, 728, 969
Bse118I RCCGGY 1 cut(s) 404
Bse1I ACTGG 2 cut(s) 902, 934
Bse3DI GCAATG 3 cut(s) 367, 753, 1129
BseAI TCCGGA 1 cut(s) 855
BseBI CCWGG 2 cut(s) 624, 674
BseDI CCNNGG 2 cut(s) 108, 712
BseGI GGATG 2 cut(s) 791, 1069
BseLI CCNNNNNNNGG 4 cut(s) 32, 33, 728, 969
BseMI GCAATG 3 cut(s) 367, 753, 1129
BseMII CTCAG 5 cut(s) 216, 463, 482, 497, 1061
BseNI ACTGG 2 cut(s) 902, 934
BseRI GAGGAG 5 cut(s) 61, 68, 73, 76, 456
BseXI GCAGC 2 cut(s) 474, 953
Bsh1236I CGCG 2 cut(s) 110, 348
BshFI GGCC 3 cut(s) 207, 222, 962
BshNI GGYRCC 1 cut(s) 757
BsiHKAI GWGCWC 2 cut(s) 426, 834
BsiSI CCGG 3 cut(s) 405, 761, 856
BslI CCNNNNNNNGG 4 cut(s) 32, 33, 728, 969
BsmAI GTCTC 4 cut(s) 232, 674, 768, 1050
BsmBI CGTCTC 2 cut(s) 232, 1050
BsmI GAATGC 1 cut(s) 907
BsnI GGCC 3 cut(s) 207, 222, 962
Bso31I GGTCTC 1 cut(s) 768
Bsp1286I GDGCHC 2 cut(s) 426, 834
Bsp13I TCCGGA 1 cut(s) 855
Bsp1407I TGTACA 1 cut(s) 1089
Bsp143I GATC 1 cut(s) 611
BspANI GGCC 3 cut(s) 207, 222, 962
BspCNI CTCAG 5 cut(s) 215, 462, 481, 496, 1060
BspEI TCCGGA 1 cut(s) 855
BspFNI CGCG 2 cut(s) 110, 348
BspLI GGNNCC 2 cut(s) 409, 759
BspPI GGATC 1 cut(s) 619
BspT107I GGYRCC 1 cut(s) 757
BspTNI GGTCTC 1 cut(s) 768
BsrBI CCGCTC 1 cut(s) 975
BsrDI GCAATG 3 cut(s) 367, 753, 1129
BsrFI RCCGGY 1 cut(s) 404
BsrGI TGTACA 1 cut(s) 1089
BsrI ACTGG 2 cut(s) 902, 934
BssAI RCCGGY 1 cut(s) 404
BssECI CCNNGG 2 cut(s) 108, 712
BssMI GATC 1 cut(s) 611
BssNI GRCGYC 2 cut(s) 289, 758
BssSI CACGAG 1 cut(s) 425
BssT1I CCWWGG 1 cut(s) 712
Bst2BI CACGAG 1 cut(s) 425
Bst2UI CCWGG 2 cut(s) 624, 674
Bst4CI ACNGT 3 cut(s) 864, 916, 1030
Bst6I CTCTTC 2 cut(s) 16, 917
BstACI GRCGYC 2 cut(s) 289, 758
BstAUI TGTACA 1 cut(s) 1089
BstC8I GCNNGC 4 cut(s) 406, 737, 964, 1016
BstDEI CTNAG 5 cut(s) 202, 449, 468, 483, 1047
BstDSI CCRYGG 1 cut(s) 108
BstEII GGTNACC 1 cut(s) 977
BstF5I GGATG 2 cut(s) 791, 1069
BstFNI CGCG 2 cut(s) 110, 348
BstH2I RGCGCY 1 cut(s) 761
BstHHI GCGC 2 cut(s) 350, 760
BstKTI GATC 1 cut(s) 614
BstMAI GTCTC 4 cut(s) 232, 674, 768, 1050
BstMBI GATC 1 cut(s) 611
BstMWI GCNNNNNNNGC 5 cut(s) 213, 219, 334, 881, 972
BstNI CCWGG 2 cut(s) 624, 674
BstNSI RCATGY 1 cut(s) 1011
BstPI GGTNACC 1 cut(s) 977
BstSCI CCNGG 2 cut(s) 622, 672
BstSFI CTRYAG 1 cut(s) 814
BstUI CGCG 2 cut(s) 110, 348
BstV1I GCAGC 2 cut(s) 474, 953
BstV2I GAAGAC 1 cut(s) 771
BstX2I RGATCY 1 cut(s) 611
BstYI RGATCY 1 cut(s) 611
BsuRI GGCC 3 cut(s) 207, 222, 962
BtgI CCRYGG 1 cut(s) 108
BtrI CACGTC 2 cut(s) 226, 997
BtsCI GGATG 2 cut(s) 791, 1069
BtsIMutI CAGTG 1 cut(s) 1077
Cac8I GCNNGC 4 cut(s) 406, 737, 964, 1016
CfoI GCGC 2 cut(s) 350, 760
Cfr10I RCCGGY 1 cut(s) 404
Cfr13I GGNCC 3 cut(s) 195, 206, 536
Cfr42I CCGCGG 1 cut(s) 111
CseI GACGC 2 cut(s) 297, 787
Csp6I GTAC 2 cut(s) 1051, 1090
CviAII CATG 3 cut(s) 872, 1008, 1087
CviQI GTAC 2 cut(s) 1051, 1090
DdeI CTNAG 5 cut(s) 202, 449, 468, 483, 1047
DinI GGCGCC 1 cut(s) 759
DpnI GATC 1 cut(s) 613
DpnII GATC 1 cut(s) 611
DriI GACNNNNNGTC 1 cut(s) 293
EaeI YGGCCR 1 cut(s) 220
Eam1104I CTCTTC 2 cut(s) 16, 917
Eam1105I GACNNNNNGTC 1 cut(s) 293
EarI CTCTTC 2 cut(s) 16, 917
EciI GGCGGA 2 cut(s) 85, 91
Ecl136II GAGCTC 1 cut(s) 832
Eco130I CCWWGG 1 cut(s) 712
Eco147I AGGCCT 1 cut(s) 962
Eco24I GRGCYC 1 cut(s) 834
Eco31I GGTCTC 1 cut(s) 768
Eco47I GGWCC 2 cut(s) 195, 536
Eco53kI GAGCTC 1 cut(s) 832
Eco57I CTGAAG 1 cut(s) 1101
Eco91I GGTNACC 1 cut(s) 977
EcoICRI GAGCTC 1 cut(s) 832
EcoO109I RGGNCCY 1 cut(s) 206
EcoO65I GGTNACC 1 cut(s) 977
EcoRII CCWGG 2 cut(s) 622, 672
EcoT14I CCWWGG 1 cut(s) 712
EcoT22I ATGCAT 1 cut(s) 907
EcoT38I GRGCYC 1 cut(s) 834
EgeI GGCGCC 1 cut(s) 759
EheI GGCGCC 1 cut(s) 759
ErhI CCWWGG 1 cut(s) 712
Esp3I CGTCTC 2 cut(s) 232, 1050
FaeI CATG 3 cut(s) 875, 1011, 1090
FatI CATG 3 cut(s) 871, 1007, 1086
FauI CCCGC 1 cut(s) 962
Fnu4HI GCNGC 4 cut(s) 214, 488, 967, 1012
FokI GGATG 2 cut(s) 798, 1076
FriOI GRGCYC 1 cut(s) 834
Fsp4HI GCNGC 4 cut(s) 214, 488, 967, 1012
FspBI CTAG 3 cut(s) 210, 293, 663
GlaI GCGC 2 cut(s) 349, 759
GluI GCNGC 4 cut(s) 214, 488, 967, 1012
GsuI CTGGAG 1 cut(s) 656
HaeII RGCGCY 1 cut(s) 761
HaeIII GGCC 3 cut(s) 207, 222, 962
HapII CCGG 3 cut(s) 405, 761, 856
HgaI GACGC 2 cut(s) 297, 787
HhaI GCGC 2 cut(s) 350, 760
Hin1I GRCGYC 2 cut(s) 289, 758
Hin1II CATG 3 cut(s) 875, 1011, 1090
Hin6I GCGC 2 cut(s) 348, 758
HinP1I GCGC 2 cut(s) 348, 758
HincII GTYRAC 2 cut(s) 391, 802
HindII GTYRAC 2 cut(s) 391, 802
HindIII AAGCTT 1 cut(s) 641
HinfI GANTC 6 cut(s) 167, 314, 374, 481, 925, 954
HpaI GTTAAC 1 cut(s) 391
HpaII CCGG 3 cut(s) 405, 761, 856
HphI GGTGA 7 cut(s) 49, 176, 241, 251, 638, 989, 1124
Hpy166II GTNNAC 2 cut(s) 391, 802
Hpy188I TCNGA 6 cut(s) 283, 616, 639, 785, 930, 1105
Hpy188III TCNNGA 4 cut(s) 145, 478, 793, 856
Hpy8I GTNNAC 2 cut(s) 391, 802
Hpy99I CGWCG 3 cut(s) 287, 311, 404
HpyAV CCTTC 3 cut(s) 151, 682, 1029
HpyCH4III ACNGT 3 cut(s) 864, 916, 1030
HpyCH4IV ACGT 5 cut(s) 48, 225, 702, 752, 996
HpyCH4V TGCA 6 cut(s) 337, 525, 546, 560, 847, 905
HpyF10VI GCNNNNNNNGC 5 cut(s) 213, 219, 334, 881, 972
HpyF3I CTNAG 5 cut(s) 202, 449, 468, 483, 1047
HpySE526I ACGT 5 cut(s) 48, 225, 702, 752, 996
Hsp92I GRCGYC 2 cut(s) 289, 758
Hsp92II CATG 3 cut(s) 875, 1011, 1090
HspAI GCGC 2 cut(s) 348, 758
KasI GGCGCC 1 cut(s) 757
Kpn2I TCCGGA 1 cut(s) 855
KroI GCCGGC 1 cut(s) 404
KroNI GCCGGC 1 cut(s) 406
KspAI GTTAAC 1 cut(s) 391
KspI CCGCGG 1 cut(s) 111
Kzo9I GATC 1 cut(s) 611
LmnI GCTCC 4 cut(s) 241, 413, 837, 972
Lsp1109I GCAGC 2 cut(s) 474, 953
LweI GCATC 3 cut(s) 255, 547, 789
MaeI CTAG 3 cut(s) 210, 293, 663
MaeII ACGT 5 cut(s) 48, 225, 702, 752, 996
MaeIII GTNAC 8 cut(s) 49, 112, 164, 315, 658, 977, 1145, 1169
MalI GATC 1 cut(s) 613
MbiI CCGCTC 1 cut(s) 975
MboI GATC 1 cut(s) 611
MboII GAAGA 6 cut(s) 3, 6, 9, 451, 776, 934
MflI RGATCY 1 cut(s) 611
MhlI GDGCHC 2 cut(s) 426, 834
MluCI AATT 5 cut(s) 39, 548, 579, 633, 1057
Mly113I GGCGCC 1 cut(s) 758
MlyI GAGTC 5 cut(s) 161, 323, 368, 475, 934
MmeI TCCRAC 4 cut(s) 306, 511, 553, 1134
Mph1103I ATGCAT 1 cut(s) 907
MroI TCCGGA 1 cut(s) 855
MroNI GCCGGC 1 cut(s) 404
MseI TTAA 2 cut(s) 390, 683
MslI CAYNNNNRTG 1 cut(s) 917
MspA1I CMGCKG 2 cut(s) 110, 487
MspI CCGG 3 cut(s) 405, 761, 856
MspR9I CCNGG 2 cut(s) 624, 674
Mva1269I GAATGC 1 cut(s) 907
MvaI CCWGG 2 cut(s) 624, 674
MvnI CGCG 2 cut(s) 110, 348
MwoI GCNNNNNNNGC 5 cut(s) 213, 219, 334, 881, 972
NaeI GCCGGC 1 cut(s) 406
NarI GGCGCC 1 cut(s) 758
NdeII GATC 1 cut(s) 611
NgoMIV GCCGGC 1 cut(s) 404
NlaIII CATG 3 cut(s) 875, 1011, 1090
NlaIV GGNNCC 2 cut(s) 409, 759
NmeAIII GCCGAG 1 cut(s) 337
NmuCI GTSAC 4 cut(s) 112, 164, 315, 977
NsiI ATGCAT 1 cut(s) 907
NspI RCATGY 1 cut(s) 1011
PceI AGGCCT 1 cut(s) 962
PctI GAATGC 1 cut(s) 907
PdiI GCCGGC 1 cut(s) 406
PfeI GAWTC 1 cut(s) 954
PflFI GACNNNGTC 1 cut(s) 305
PkrI GCNGC 4 cut(s) 215, 489, 968, 1013
PleI GAGTC 5 cut(s) 161, 322, 368, 475, 933
PluTI GGCGCC 1 cut(s) 761
PpsI GAGTC 5 cut(s) 161, 322, 368, 475, 933
Psp124BI GAGCTC 1 cut(s) 834
Psp6I CCWGG 2 cut(s) 622, 672
PspEI GGTNACC 1 cut(s) 977
PspGI CCWGG 2 cut(s) 622, 672
PspN4I GGNNCC 2 cut(s) 409, 759
PspPI GGNCC 3 cut(s) 195, 206, 536
PsuI RGATCY 1 cut(s) 611
PsyI GACNNNGTC 1 cut(s) 305
PvuII CAGCTG 1 cut(s) 487
RsaI GTAC 2 cut(s) 1052, 1091
RsaNI GTAC 2 cut(s) 1051, 1090
RseI CAYNNNNRTG 1 cut(s) 917
SacI GAGCTC 1 cut(s) 834
SacII CCGCGG 1 cut(s) 111
SaqAI TTAA 2 cut(s) 390, 683
SatI GCNGC 4 cut(s) 214, 488, 967, 1012
Sau3AI GATC 1 cut(s) 611
Sau96I GGNCC 3 cut(s) 195, 206, 536
SchI GAGTC 5 cut(s) 161, 323, 368, 475, 934
ScrFI CCNGG 2 cut(s) 624, 674
SduI GDGCHC 2 cut(s) 426, 834
SfaNI GCATC 3 cut(s) 255, 547, 789
SfcI CTRYAG 1 cut(s) 814
SfoI GGCGCC 1 cut(s) 759
Sfr303I CCGCGG 1 cut(s) 111
SgrBI CCGCGG 1 cut(s) 111
SinI GGWCC 2 cut(s) 195, 536
SmiMI CAYNNNNRTG 1 cut(s) 917
SmlI CTYRAG 1 cut(s) 827
SmoI CTYRAG 1 cut(s) 827
SpeI ACTAGT 1 cut(s) 662
Sse9I AATT 5 cut(s) 39, 548, 579, 633, 1057
SseBI AGGCCT 1 cut(s) 962
SspDI GGCGCC 1 cut(s) 757
SspMI CTAG 3 cut(s) 210, 293, 663
SstI GAGCTC 1 cut(s) 834
StuI AGGCCT 1 cut(s) 962
StyD4I CCNGG 2 cut(s) 622, 672
StyI CCWWGG 1 cut(s) 712
TaaI ACNGT 3 cut(s) 864, 916, 1030
TaiI ACGT 5 cut(s) 51, 228, 705, 755, 999
TaqI TCGA 1 cut(s) 356
TasI AATT 5 cut(s) 39, 548, 579, 633, 1057
TatI WGTACW 2 cut(s) 1050, 1089
TauI GCSGC 2 cut(s) 216, 1014
TfiI GAWTC 1 cut(s) 954
Tru1I TTAA 2 cut(s) 390, 683
Tru9I TTAA 2 cut(s) 390, 683
TscAI CASTG 1 cut(s) 1084
TseFI GTSAC 4 cut(s) 112, 164, 315, 977
TseI GCWGC 2 cut(s) 487, 966
Tsp45I GTSAC 4 cut(s) 112, 164, 315, 977
TspDTI ATGAA 3 cut(s) 9, 935, 1061
TspGWI ACGGA 2 cut(s) 300, 399
TspRI CASTG 1 cut(s) 1084
Tth111I GACNNNGTC 1 cut(s) 305
VpaK11BI GGWCC 2 cut(s) 195, 536
XapI RAATTY 1 cut(s) 548
XceI RCATGY 1 cut(s) 1011
XspI CTAG 3 cut(s) 210, 293, 663
Zsp2I ATGCAT 1 cut(s) 907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.