pycom06g15170

Cell division cycle protein 48 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0013824
Physical Location & Seq
Reverse (-)
19766773 .. 19770753
3981 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2394 bp
ATGGCGCCACCTAGTCCATCTGCTCCTGATCAATCCCAGCCGTCTTCGTCCTTGGACCCGAAAAGTAACAAGAAAGATTTTTCGACCGCCATTTCGGAACGAAAGAAGTCTCCTAATCGCCTTGTTGTTGATGAAGCTGTCAATGATGATAATTCTGTGATTTCCTTGCACCCTTCTGCAATGGAAAAGCTTCAGTTTTTCCGTGGAGACACTGTTTTAATTAAGGGGAAGAAAAGGAGAGACACAATTTGCATTGTTATGGCTGATAACGAATGCGAGGAGCCGAAAATCAGAATGAACAAAGTTGTGAGAGCTAATCTTAGGGTTCGCCTCGGGGATATTGTGTCTGTTCATCTCTGCCATGATGTCAAGTATGGAAAGAAGGTTCACATCCTTCCAATCGATGATACAATTGAAGGCGTGACAGGAAGCTTATTCGATGCATATTTGAAGCCATACTTTGCGGAAGCTTACCGCCCTGTGAGGAAGGGTGATCTTTTCCTGGTTCAGGGCATGATGCGAAGCATTGAGTTCAAGATCATTGAGACAGACCCTGATGAGTACTGCATTGTTGCACCGGATACTGAGATTTTCTGTGAGGGAGAGCCTGTCAAACGCGAGGACGAGGAAAGACAAGAGGATGTAGGATATGATGATGTTGGAGGTGTTCGAAAGCAAATGGCTCAGATACGCGAGTTAGTAGAACTGCCTCTTAGACATCCACAGCTCTTCGAATCGATTGGAGTGAAACCCCCGAAGGGGATTTTACTCTATGGGCCACCCGGGACGGGGAAAACATTGATTGCAAGGGCTGTAGCAAATGAGACTGGAGCGTTCTTCTTTTCGATCAATGGACCTGAAATAATGTCCAAGATGGCAGGTGAAAGTGAAAGCAACTTGAGGAAGGCGTTTGAGGAAGCTGAAAAGAATGCACCCTCAATCATTTTTATTGATGAGATAGATTCTATTGCTCCGAAGAGGGAAAAGACCCACGGTGAAGTGGGAAGGCGTATAGTTTCCCAGCTCCTGACTTTAATGGACGGCCTTAAGTCCCGTGCGCATGTCATTGTAATGGGAGCAACGAATAGGCCAAACAGCATCGACCCTGCTCTAAGGAGATTTGGAAGATTCGATAGAGAGATTGACATTGGTGTCCCGGATGAAGTTGGAAGATTGGAAGTTTTGCGAATCCATACAAAGAGGATGAAACTTGCAGATGATGTTGATCTCGAACGAGTTGCAAAAGATACCCATGGTTTTGTTGGTGCAGATCTTGCCGCTCTCTCCACAGAGGCTGCGCTCCAGTGCATCCGTGAAAAAATGGACGTTATTGACTTGGAAGACGATACGATTGATGCTGAGGTGTTGAATTCGATGGCTGTGACGAATGAACACTTCCAGACCGCACTGTCCTCTACAAATCCTTCTGCCTTGCGCGAAACTGTTGTGGACGTACCTAATGTTTCGTGGGATGCCATTGGCGGGTTGGAGAACGTCAAAAGAGAACTTCAAGAGGTTTGCGTTCTCTTTTATGGACCACCTGGCTGTGGGAAAACCCTTCTCGCCAAGGCAATTGCCAATGAATGTCAGGCCAACTTCATCAGTATCAAAGGACCTGAGCTGCTAACAATGTGGTTCGGAGAAAGTGAGGCAAATGTGCGCGAGATATTTGACAAGGCTCGCCAATCCGCTCCTTGTGTTCTTTTCTTTGATGAGCTAGACTCAATTGCAACTCAGCGTGGCAATTCTGTGGGGGATGCTGGTGGTGCTGGAGACAGAGTCTTGAACCAACTTCTAACCGAAATGGATGGAATGAATGCAAAGAAAACCGTCTTTGTCATTGGGGCGACAAACAGGCCAGACATTATTGACCCGGCATTGCTCAGGCCTGGACGTCTTGACCAACTTATCTACATCCCGCTCCCAGATGAGGCTTCGCGCCTTCAGATTTTTAAAGCATGTCTGCGCAAGTCACCCATCTCTAAGGATATTGACCTAGCAGCTCTTGCCCGTTATACGCGCGGTTTTAGTGGTGCAGATATCACCGAGATATGTCAACGTGCCTGCAAATATGCCATCAGAGAAGACATTGAGAAGGCTATTGAGAGGCAAAGAAGAAAGAACGAGAACTCCGAAGCCATGGAGGAAGTTGAAACTGATGAGGGACCCGAGATCAAGCCAGTGCACTTTGAGGAATCGATGAAACATGCCCGAAGAAGTGTGAGTGATGCAGACATCAAGAAGTACCAGGTGTTTGCTCAGACTTTGCAACAATCTCGTGGGTTTGGATCCGAATTTCGATTTCCACAGACAGAGAACAATGGAGGAGACGCAGCTCCAACCCCATTTTCTTCAGCCACAGTTGCTGGAGAGGAAGATGATCTCTACGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
AAA CDC48_2 CDC48_N Vps4_C

Protein Analysis

798

Amino Acids

88.49

Weight (kDa)

5.14

Isoelectric Point (pI)

45.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC48_N PF02359 40 - 120 3.3e-22 Cell division protein 48 (CDC48), N-terminal domain
CDC48_2 PF02933 142 - 205 4.2e-11 Cell division protein 48 (CDC48), domain 2
RuvB_N PF05496 254 - 328 7.5e-06 Holliday junction DNA helicase RuvB P-loop domain
AAA PF00004 255 - 384 4.1e-49 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 407 - 448 5e-14 AAA+ lid domain
AAA PF00004 508 - 641 3.2e-47 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 663 - 702 3.6e-12 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013824)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09840 AT5G03340
fragaria_vesca FvH4_5g05770 FvH4_5g05770
malus_domestica MD06G1170300.v1.1 MD14G1175800.v1.1
pyrus_communis pycom06g15170 pycom14g14690
rosa_chinensis RchiOBHm_Chr7g0193051
rosa_laevigata RLG00000004299
rosa_multiflora Rmu_sc0028357.1_g000001
rosa_roxburghii Rroxscaffold_3G00262420
rosa_rugosa Rorug07G0007300
rosa_samantha Rh7AG132400 Rh7CG136400 Rh7DG134300
rosa_wichuraiana Rw7G011310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 869
AasI GACNNNNNNGTC 2 cut(s) 1151, 1408
AatII GACGTC 1 cut(s) 1897
Acc16I TGCGCA 2 cut(s) 1059, 1967
Acc36I ACCTGC 1 cut(s) 869
AccB1I GGYRCC 1 cut(s) 4
AccBSI CCGCTC 3 cut(s) 1280, 1691, 1921
AccII CGCG 7 cut(s) 618, 693, 1437, 1662, 1939, 2020, 2022
AciI CCGC 9 cut(s) 87, 464, 475, 1278, 1404, 1482, 1689, 1919, 2022
AclWI GGATC 2 cut(s) 2283, 2296
AcsI RAATTY 2 cut(s) 1369, 2294
AcuI CTGAAG 3 cut(s) 176, 1928, 2337
AcyI GRCGYC 2 cut(s) 5, 1894
AfaI GTAC 3 cut(s) 563, 1455, 2246
AfiI CCNNNNNNNGG 8 cut(s) 508, 758, 759, 788, 789, 1482, 1547, 1930
AflII CTTAAG 1 cut(s) 1046
AgsI TTSAA 7 cut(s) 416, 451, 535, 1369, 1511, 1786, 2153
AjnI CCWGG 4 cut(s) 501, 1540, 1888, 2247
AjuI GAANNNNNNNTTGG 2 cut(s) 2332, 2364
AloI GAACNNNNNNTCC 4 cut(s) 369, 401, 2114, 2146
Alw21I GWGCWC 1 cut(s) 2187
Alw26I GTCTC 7 cut(s) 114, 201, 234, 539, 818, 1767, 2322
Alw44I GTGCAC 1 cut(s) 2183
AlwI GGATC 2 cut(s) 2283, 2296
AlwNI CAGNNNCTG 4 cut(s) 554, 1027, 1295, 2366
Ama87I CYCGRG 3 cut(s) 332, 782, 2168
AoxI GGCC 6 cut(s) 776, 1042, 1088, 1590, 1856, 1886
ApaLI GTGCAC 1 cut(s) 2183
ApeKI GCWGC 4 cut(s) 1295, 1621, 2000, 2333
ApoI RAATTY 2 cut(s) 1369, 2294
ArsI GACNNNNNNTTYG 2 cut(s) 76, 108
Asp700I GAANNNNTTC 1 cut(s) 189
AspLEI GCGC 8 cut(s) 7, 1060, 1300, 1437, 1662, 1941, 1968, 2022
AspS9I GGNCC 6 cut(s) 55, 776, 854, 1535, 1613, 2165
AsuC2I CCSGG 4 cut(s) 783, 784, 1157, 1874
AsuHPI GGTGA 5 cut(s) 503, 893, 1007, 1965, 2035
AsuII TTCGAA 2 cut(s) 670, 732
AvaI CYCGRG 3 cut(s) 332, 782, 2168
AvaII GGWCC 5 cut(s) 55, 854, 1535, 1613, 2165
BaeGI GKGCMC 1 cut(s) 2187
BaeI ACNNNNGTAYC 2 cut(s) 680, 713
BamHI GGATCC 1 cut(s) 2288
BanI GGYRCC 1 cut(s) 4
BauI CACGAG 1 cut(s) 2277
BbsI GAAGAC 3 cut(s) 36, 1347, 2091
Bbv12I GWGCWC 1 cut(s) 2187
BbvCI CCTCAGC 1 cut(s) 1359
BbvI GCAGC 4 cut(s) 1282, 1608, 2012, 2345
BccI CCATC 6 cut(s) 25, 868, 1369, 1802, 1985, 2084
BceAI ACGGC 2 cut(s) 25, 1057
BcgI CGANNNNNNTGC 4 cut(s) 1220, 1254, 2258, 2292
BciT130I CCWGG 4 cut(s) 503, 1542, 1890, 2249
BciVI GTATCC 1 cut(s) 574
BclI TGATCA 1 cut(s) 28
BcnI CCSGG 4 cut(s) 783, 784, 1157, 1874
BcoDI GTCTC 7 cut(s) 114, 201, 234, 539, 818, 1767, 2322
BfaI CTAG 3 cut(s) 12, 1718, 1997
BfmI CTRYAG 1 cut(s) 813
BfoI RGCGCY 1 cut(s) 8
BfrI CTTAAG 1 cut(s) 1046
BfuAI ACCTGC 1 cut(s) 869
BfuI GTATCC 1 cut(s) 574
BglII AGATCT 1 cut(s) 1270
BisI GCNGC 5 cut(s) 1278, 1296, 1622, 2001, 2334
BlsI GCNGC 5 cut(s) 1279, 1297, 1623, 2002, 2335
BmcAI AGTACT 1 cut(s) 563
Bme1390I CCNGG 8 cut(s) 503, 783, 784, 1157, 1542, 1874, 1890, 2249
Bme18I GGWCC 5 cut(s) 55, 854, 1535, 1613, 2165
BmeT110I CYCGRG 3 cut(s) 332, 782, 2168
BmgT120I GGNCC 6 cut(s) 55, 776, 854, 1535, 1613, 2165
BmiI GGNNCC 6 cut(s) 6, 57, 282, 2166, 2167, 2290
BmrFI CCNGG 8 cut(s) 503, 783, 784, 1157, 1542, 1874, 1890, 2249
BmsI GCATC 8 cut(s) 430, 507, 1107, 1317, 1345, 1462, 1747, 2218
BpiI GAAGAC 3 cut(s) 36, 1347, 2091
BplI GAGNNNNNCTC 2 cut(s) 1706, 1738
BpmI CTGGAG 4 cut(s) 849, 1286, 1791, 2388
Bpu10I CCTNAGC 3 cut(s) 1359, 1617, 1883
Bpu14I TTCGAA 2 cut(s) 670, 732
BpuEI CTTGAG 1 cut(s) 919
BpuMI CCSGG 4 cut(s) 783, 784, 1157, 1874
Bsa29I ATCGAT 3 cut(s) 402, 737, 2198
BsaBI GATNNNNATC 1 cut(s) 1224
BsaHI GRCGYC 2 cut(s) 5, 1894
BsaJI CCNNGG 8 cut(s) 51, 202, 331, 782, 991, 1252, 1566, 2139
BsaWI WCCGGW 1 cut(s) 577
BsaXI ACNNNNNCTCC 8 cut(s) 198, 228, 594, 624, 1764, 1794, 2114, 2144
Bsc4I CCNNNNNNNGG 8 cut(s) 508, 758, 759, 788, 789, 1482, 1547, 1930
Bse1I ACTGG 3 cut(s) 832, 1303, 2180
Bse3DI GCAATG 2 cut(s) 186, 1877
Bse8I GATNNNNATC 1 cut(s) 1224
BseBI CCWGG 4 cut(s) 503, 1542, 1890, 2249
BseCI ATCGAT 3 cut(s) 402, 737, 2198
BseDI CCNNGG 8 cut(s) 51, 202, 331, 782, 991, 1252, 1566, 2139
BseJI GATNNNNATC 1 cut(s) 1224
BseLI CCNNNNNNNGG 8 cut(s) 508, 758, 759, 788, 789, 1482, 1547, 1930
BseMI GCAATG 2 cut(s) 186, 1877
BseMII CTCAG 7 cut(s) 576, 698, 1350, 1608, 1748, 1897, 2273
BseNI ACTGG 3 cut(s) 832, 1303, 2180
BseRI GAGGAG 2 cut(s) 293, 2340
BseSI GKGCMC 1 cut(s) 2187
BseXI GCAGC 4 cut(s) 1282, 1608, 2012, 2345
BseYI CCCAGC 2 cut(s) 36, 1020
BsgI GTGCAG 2 cut(s) 1287, 2055
Bsh1236I CGCG 7 cut(s) 618, 693, 1437, 1662, 1939, 2020, 2022
Bsh1285I CGRYCG 1 cut(s) 87
BshFI GGCC 6 cut(s) 778, 1044, 1090, 1592, 1858, 1888
BshNI GGYRCC 1 cut(s) 4
BshVI ATCGAT 3 cut(s) 402, 737, 2198
BsiEI CGRYCG 1 cut(s) 87
BsiHKAI GWGCWC 1 cut(s) 2187
BsiHKCI CYCGRG 3 cut(s) 332, 782, 2168
BsiSI CCGG 4 cut(s) 578, 783, 1157, 1874
BslFI GGGAC 4 cut(s) 799, 1036, 1139, 2178
BslI CCNNNNNNNGG 8 cut(s) 508, 758, 759, 788, 789, 1482, 1547, 1930
BsmAI GTCTC 7 cut(s) 114, 201, 234, 539, 818, 1767, 2322
BsmBI CGTCTC 1 cut(s) 2322
BsmFI GGGAC 4 cut(s) 799, 1036, 1139, 2178
BsmI GAATGC 3 cut(s) 278, 934, 1822
BsnI GGCC 6 cut(s) 778, 1044, 1090, 1592, 1858, 1888
BsoBI CYCGRG 3 cut(s) 332, 782, 2168
Bsp119I TTCGAA 2 cut(s) 670, 732
Bsp1286I GDGCHC 1 cut(s) 2187
Bsp143I GATC 9 cut(s) 28, 493, 537, 846, 1225, 1270, 2172, 2288, 2380
Bsp19I CCATGG 2 cut(s) 1252, 2139
BspACI CCGC 9 cut(s) 87, 464, 475, 1278, 1404, 1482, 1689, 1919, 2022
BspANI GGCC 6 cut(s) 778, 1044, 1090, 1592, 1858, 1888
BspCNI CTCAG 7 cut(s) 577, 697, 1351, 1609, 1747, 1896, 2272
BspDI ATCGAT 3 cut(s) 402, 737, 2198
BspFNI CGCG 7 cut(s) 618, 693, 1437, 1662, 1939, 2020, 2022
BspLI GGNNCC 6 cut(s) 6, 57, 282, 2166, 2167, 2290
BspMI ACCTGC 1 cut(s) 869
BspPI GGATC 2 cut(s) 2283, 2296
BspQI GCTCTTC 1 cut(s) 734
BspT104I TTCGAA 2 cut(s) 670, 732
BspT107I GGYRCC 1 cut(s) 4
BspTI CTTAAG 1 cut(s) 1046
BsrBI CCGCTC 3 cut(s) 1280, 1691, 1921
BsrDI GCAATG 2 cut(s) 186, 1877
BsrI ACTGG 3 cut(s) 832, 1303, 2180
BssECI CCNNGG 8 cut(s) 51, 202, 331, 782, 991, 1252, 1566, 2139
BssMI GATC 9 cut(s) 28, 493, 537, 846, 1225, 1270, 2172, 2288, 2380
BssNI GRCGYC 2 cut(s) 5, 1894
BssSI CACGAG 1 cut(s) 2277
BssT1I CCWWGG 4 cut(s) 51, 1252, 1566, 2139
Bst2BI CACGAG 1 cut(s) 2277
Bst2UI CCWGG 4 cut(s) 503, 1542, 1890, 2249
Bst4CI ACNGT 6 cut(s) 214, 995, 1410, 1444, 1831, 2362
Bst6I CTCTTC 2 cut(s) 734, 971
BstACI GRCGYC 2 cut(s) 5, 1894
BstAFI CTTAAG 1 cut(s) 1046
BstAPI GCANNNNNTGC 2 cut(s) 1274, 2006
BstBI TTCGAA 2 cut(s) 670, 732
BstC8I GCNNGC 2 cut(s) 1681, 2065
BstDSI CCRYGG 4 cut(s) 202, 991, 1252, 2139
BstENI CCTNNNNNAGG 1 cut(s) 506
BstFNI CGCG 7 cut(s) 618, 693, 1437, 1662, 1939, 2020, 2022
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 8 cut(s) 7, 1060, 1300, 1437, 1662, 1941, 1968, 2022
BstKTI GATC 9 cut(s) 31, 496, 540, 849, 1228, 1273, 2175, 2291, 2383
BstMAI GTCTC 7 cut(s) 114, 201, 234, 539, 818, 1767, 2322
BstMBI GATC 9 cut(s) 28, 493, 537, 846, 1225, 1270, 2172, 2288, 2380
BstMCI CGRYCG 1 cut(s) 87
BstMWI GCNNNNNNNGC 4 cut(s) 1274, 1766, 2006, 2363
BstNI CCWGG 4 cut(s) 503, 1542, 1890, 2249
BstNSI RCATGY 3 cut(s) 1064, 1962, 2210
BstSCI CCNGG 8 cut(s) 501, 781, 782, 1155, 1540, 1872, 1888, 2247
BstSFI CTRYAG 1 cut(s) 813
BstSLI GKGCMC 1 cut(s) 2187
BstUI CGCG 7 cut(s) 618, 693, 1437, 1662, 1939, 2020, 2022
BstV1I GCAGC 4 cut(s) 1282, 1608, 2012, 2345
BstV2I GAAGAC 3 cut(s) 36, 1347, 2091
BstX2I RGATCY 2 cut(s) 1270, 2288
BstYI RGATCY 2 cut(s) 1270, 2288
Bsu15I ATCGAT 3 cut(s) 402, 737, 2198
BsuI GTATCC 1 cut(s) 574
BsuRI GGCC 6 cut(s) 778, 1044, 1090, 1592, 1858, 1888
BsuTUI ATCGAT 3 cut(s) 402, 737, 2198
BtgI CCRYGG 4 cut(s) 202, 991, 1252, 2139
BtsIMutI CAGTG 4 cut(s) 210, 1310, 1406, 2187
BveI ACCTGC 1 cut(s) 869
Cac8I GCNNGC 2 cut(s) 1681, 2065
CaiI CAGNNNCTG 4 cut(s) 554, 1027, 1295, 2366
CfoI GCGC 8 cut(s) 7, 1060, 1300, 1437, 1662, 1941, 1968, 2022
Cfr13I GGNCC 6 cut(s) 55, 776, 854, 1535, 1613, 2165
Cfr9I CCCGGG 1 cut(s) 782
ClaI ATCGAT 3 cut(s) 402, 737, 2198
CseI GACGC 1 cut(s) 2339
CsiI ACCWGGT 1 cut(s) 2247
Csp6I GTAC 3 cut(s) 562, 1454, 2245
CviAII CATG 7 cut(s) 362, 514, 1061, 1253, 1959, 2140, 2207
CviQI GTAC 3 cut(s) 562, 1454, 2245
DinI GGCGCC 1 cut(s) 6
DpnI GATC 9 cut(s) 30, 495, 539, 848, 1227, 1272, 2174, 2290, 2382
DpnII GATC 9 cut(s) 28, 493, 537, 846, 1225, 1270, 2172, 2288, 2380
DraI TTTAAA 1 cut(s) 1954
DrdI GACNNNNNNGTC 2 cut(s) 1151, 1408
DseDI GACNNNNNNGTC 2 cut(s) 1151, 1408
Eam1104I CTCTTC 2 cut(s) 734, 971
EarI CTCTTC 2 cut(s) 734, 971
Eco130I CCWWGG 4 cut(s) 51, 1252, 1566, 2139
Eco147I AGGCCT 1 cut(s) 1888
Eco32I GATATC 1 cut(s) 2041
Eco47I GGWCC 5 cut(s) 55, 854, 1535, 1613, 2165
Eco57I CTGAAG 3 cut(s) 176, 1928, 2337
Eco88I CYCGRG 3 cut(s) 332, 782, 2168
EcoNI CCTNNNNNAGG 1 cut(s) 506
EcoO109I RGGNCCY 2 cut(s) 1613, 2165
EcoRI GAATTC 1 cut(s) 1369
EcoRII CCWGG 4 cut(s) 501, 1540, 1888, 2247
EcoRV GATATC 1 cut(s) 2041
EcoT14I CCWWGG 4 cut(s) 51, 1252, 1566, 2139
EcoT22I ATGCAT 1 cut(s) 445
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 4 cut(s) 51, 1252, 1566, 2139
Esp3I CGTCTC 1 cut(s) 2322
FaeI CATG 7 cut(s) 365, 517, 1064, 1256, 1962, 2143, 2210
FalI AAGNNNNNCTT 2 cut(s) 443, 475
FaqI GGGAC 4 cut(s) 799, 1036, 1139, 2178
FatI CATG 7 cut(s) 361, 513, 1060, 1252, 1958, 2139, 2206
FauI CCCGC 2 cut(s) 1475, 1926
FbaI TGATCA 1 cut(s) 28
Fnu4HI GCNGC 5 cut(s) 1278, 1296, 1622, 2001, 2334
Fsp4HI GCNGC 5 cut(s) 1278, 1296, 1622, 2001, 2334
FspAI RTGCGCAY 1 cut(s) 1059
FspBI CTAG 3 cut(s) 12, 1718, 1997
FspI TGCGCA 2 cut(s) 1059, 1967
GlaI GCGC 8 cut(s) 6, 1059, 1299, 1436, 1661, 1940, 1967, 2021
GluI GCNGC 5 cut(s) 1278, 1296, 1622, 2001, 2334
GsaI CCCAGC 2 cut(s) 40, 1024
GsuI CTGGAG 4 cut(s) 849, 1286, 1791, 2388
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 6 cut(s) 778, 1044, 1090, 1592, 1858, 1888
HapII CCGG 4 cut(s) 578, 783, 1157, 1874
HgaI GACGC 1 cut(s) 2339
HhaI GCGC 8 cut(s) 7, 1060, 1300, 1437, 1662, 1941, 1968, 2022
Hin1I GRCGYC 2 cut(s) 5, 1894
Hin1II CATG 7 cut(s) 365, 517, 1064, 1256, 1962, 2143, 2210
Hin6I GCGC 8 cut(s) 5, 1058, 1298, 1435, 1660, 1939, 1966, 2020
HinP1I GCGC 8 cut(s) 5, 1058, 1298, 1435, 1660, 1939, 1966, 2020
HincII GTYRAC 1 cut(s) 2057
HindII GTYRAC 1 cut(s) 2057
HindIII AAGCTT 3 cut(s) 188, 430, 468
HinfI GANTC 7 cut(s) 734, 962, 1128, 1188, 1721, 1779, 2195
HpaII CCGG 4 cut(s) 578, 783, 1157, 1874
HphI GGTGA 5 cut(s) 503, 893, 1007, 1965, 2035
Hpy166II GTNNAC 4 cut(s) 388, 1450, 2057, 2185
Hpy188III TCNNGA 9 cut(s) 26, 535, 1027, 1229, 1399, 1511, 1783, 1898, 2239
Hpy8I GTNNAC 4 cut(s) 388, 1450, 2057, 2185
HpyCH4III ACNGT 6 cut(s) 214, 995, 1410, 1444, 1831, 2362
HpyCH4IV ACGT 5 cut(s) 1326, 1452, 1494, 1894, 2059
HpyF10VI GCNNNNNNNGC 4 cut(s) 1274, 1766, 2006, 2363
HpySE526I ACGT 5 cut(s) 1326, 1452, 1494, 1894, 2059
Hsp92I GRCGYC 2 cut(s) 5, 1894
Hsp92II CATG 7 cut(s) 365, 517, 1064, 1256, 1962, 2143, 2210
HspAI GCGC 8 cut(s) 5, 1058, 1298, 1435, 1660, 1939, 1966, 2020
KasI GGCGCC 1 cut(s) 4
KflI GGGWCCC 1 cut(s) 2165
Ksp22I TGATCA 1 cut(s) 28
Kzo9I GATC 9 cut(s) 28, 493, 537, 846, 1225, 1270, 2172, 2288, 2380
LguI GCTCTTC 1 cut(s) 734
Lsp1109I GCAGC 4 cut(s) 1282, 1608, 2012, 2345
LweI GCATC 8 cut(s) 430, 507, 1107, 1317, 1345, 1462, 1747, 2218
MabI ACCWGGT 1 cut(s) 2247
MaeI CTAG 3 cut(s) 12, 1718, 1997
MaeII ACGT 5 cut(s) 1326, 1452, 1494, 1894, 2059
MaeIII GTNAC 4 cut(s) 65, 421, 1381, 1971
MalI GATC 9 cut(s) 30, 495, 539, 848, 1227, 1272, 2174, 2290, 2382
MbiI CCGCTC 3 cut(s) 1280, 1691, 1921
MboI GATC 9 cut(s) 28, 493, 537, 846, 1225, 1270, 2172, 2288, 2380
MfeI CAATTG 3 cut(s) 411, 1572, 1725
MflI RGATCY 2 cut(s) 1270, 2288
MhlI GDGCHC 1 cut(s) 2187
MluCI AATT 9 cut(s) 151, 219, 246, 411, 1369, 1572, 1725, 1744, 2294
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 2 cut(s) 1715, 1788
MmeI TCCRAC 4 cut(s) 640, 1147, 1467, 2363
Mph1103I ATGCAT 1 cut(s) 445
MroXI GAANNNNTTC 1 cut(s) 189
MseI TTAA 5 cut(s) 218, 222, 1034, 1047, 1953
MslI CAYNNNNRTG 2 cut(s) 257, 1070
MspCI CTTAAG 1 cut(s) 1046
MspI CCGG 4 cut(s) 578, 783, 1157, 1874
MspR9I CCNGG 8 cut(s) 503, 783, 784, 1157, 1542, 1874, 1890, 2249
MunI CAATTG 3 cut(s) 411, 1572, 1725
Mva1269I GAATGC 3 cut(s) 278, 934, 1822
MvaI CCWGG 4 cut(s) 503, 1542, 1890, 2249
MvnI CGCG 7 cut(s) 618, 693, 1437, 1662, 1939, 2020, 2022
MwoI GCNNNNNNNGC 4 cut(s) 1274, 1766, 2006, 2363
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 4 cut(s) 783, 784, 1157, 1874
NcoI CCATGG 2 cut(s) 1252, 2139
NdeII GATC 9 cut(s) 28, 493, 537, 846, 1225, 1270, 2172, 2288, 2380
NlaIII CATG 7 cut(s) 365, 517, 1064, 1256, 1962, 2143, 2210
NlaIV GGNNCC 6 cut(s) 6, 57, 282, 2166, 2167, 2290
NmuCI GTSAC 3 cut(s) 421, 1381, 1971
NsbI TGCGCA 2 cut(s) 1059, 1967
NsiI ATGCAT 1 cut(s) 445
NspI RCATGY 3 cut(s) 1064, 1962, 2210
NspV TTCGAA 2 cut(s) 670, 732
PacI TTAATTAA 1 cut(s) 222
PaqCI CACCTGC 1 cut(s) 869
PceI AGGCCT 1 cut(s) 1888
PciSI GCTCTTC 1 cut(s) 734
PcsI WCGNNNNNNNCGW 1 cut(s) 2130
PctI GAATGC 3 cut(s) 278, 934, 1822
PdmI GAANNNNTTC 1 cut(s) 189
PfeI GAWTC 5 cut(s) 734, 962, 1128, 1188, 2195
PflFI GACNNNGTC 1 cut(s) 1778
PfoI TCCNGGA 1 cut(s) 1155
PkrI GCNGC 5 cut(s) 1279, 1297, 1623, 2002, 2335
PleI GAGTC 2 cut(s) 1715, 1787
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 2 cut(s) 1715, 1787
PpuMI RGGWCCY 2 cut(s) 1613, 2165
Psp5II RGGWCCY 2 cut(s) 1613, 2165
Psp6I CCWGG 4 cut(s) 501, 1540, 1888, 2247
PspFI CCCAGC 2 cut(s) 36, 1020
PspGI CCWGG 4 cut(s) 501, 1540, 1888, 2247
PspN4I GGNNCC 6 cut(s) 6, 57, 282, 2166, 2167, 2290
PspPI GGNCC 6 cut(s) 55, 776, 854, 1535, 1613, 2165
PspPPI RGGWCCY 2 cut(s) 1613, 2165
PstNI CAGNNNCTG 4 cut(s) 554, 1027, 1295, 2366
PsuI RGATCY 2 cut(s) 1270, 2288
PsyI GACNNNGTC 1 cut(s) 1778
RsaI GTAC 3 cut(s) 563, 1455, 2246
RsaNI GTAC 3 cut(s) 562, 1454, 2245
RseI CAYNNNNRTG 2 cut(s) 257, 1070
SapI GCTCTTC 1 cut(s) 734
SaqAI TTAA 5 cut(s) 218, 222, 1034, 1047, 1953
SatI GCNGC 5 cut(s) 1278, 1296, 1622, 2001, 2334
Sau3AI GATC 9 cut(s) 28, 493, 537, 846, 1225, 1270, 2172, 2288, 2380
Sau96I GGNCC 6 cut(s) 55, 776, 854, 1535, 1613, 2165
ScaI AGTACT 1 cut(s) 563
SchI GAGTC 2 cut(s) 1715, 1788
ScrFI CCNGG 8 cut(s) 503, 783, 784, 1157, 1542, 1874, 1890, 2249
SduI GDGCHC 1 cut(s) 2187
SexAI ACCWGGT 1 cut(s) 2247
SfaNI GCATC 8 cut(s) 430, 507, 1107, 1317, 1345, 1462, 1747, 2218
SfcI CTRYAG 1 cut(s) 813
SfoI GGCGCC 1 cut(s) 6
SfuI TTCGAA 2 cut(s) 670, 732
SinI GGWCC 5 cut(s) 55, 854, 1535, 1613, 2165
SmaI CCCGGG 1 cut(s) 784
SmiMI CAYNNNNRTG 2 cut(s) 257, 1070
SmlI CTYRAG 2 cut(s) 898, 1046
SmoI CTYRAG 2 cut(s) 898, 1046
Sse9I AATT 9 cut(s) 151, 219, 246, 411, 1369, 1572, 1725, 1744, 2294
SseBI AGGCCT 1 cut(s) 1888
SsiI CCGC 9 cut(s) 87, 464, 475, 1278, 1404, 1482, 1689, 1919, 2022
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 3 cut(s) 12, 1718, 1997
StuI AGGCCT 1 cut(s) 1888
StyD4I CCNGG 8 cut(s) 501, 781, 782, 1155, 1540, 1872, 1888, 2247
StyI CCWWGG 4 cut(s) 51, 1252, 1566, 2139
TaaI ACNGT 6 cut(s) 214, 995, 1410, 1444, 1831, 2362
TaiI ACGT 5 cut(s) 1329, 1455, 1497, 1897, 2062
TasI AATT 9 cut(s) 151, 219, 246, 411, 1369, 1572, 1725, 1744, 2294
TatI WGTACW 1 cut(s) 561
TauI GCSGC 1 cut(s) 1280
TfiI GAWTC 5 cut(s) 734, 962, 1128, 1188, 2195
Tru1I TTAA 5 cut(s) 218, 222, 1034, 1047, 1953
Tru9I TTAA 5 cut(s) 218, 222, 1034, 1047, 1953
TscAI CASTG 4 cut(s) 217, 1310, 1413, 2187
TseFI GTSAC 3 cut(s) 421, 1381, 1971
TseI GCWGC 4 cut(s) 1295, 1621, 2000, 2333
Tsp45I GTSAC 3 cut(s) 421, 1381, 1971
TspGWI ACGGA 2 cut(s) 191, 1301
TspMI CCCGGG 1 cut(s) 782
TspRI CASTG 4 cut(s) 217, 1310, 1413, 2187
Tth111I GACNNNGTC 1 cut(s) 1778
Vha464I CTTAAG 1 cut(s) 1046
VneI GTGCAC 1 cut(s) 2183
VpaK11BI GGWCC 5 cut(s) 55, 854, 1535, 1613, 2165
XagI CCTNNNNNAGG 1 cut(s) 506
XapI RAATTY 2 cut(s) 1369, 2294
XceI RCATGY 3 cut(s) 1064, 1962, 2210
XcmI CCANNNNNNNNNTGG 2 cut(s) 1259, 1483
XmaI CCCGGG 1 cut(s) 782
XmnI GAANNNNTTC 1 cut(s) 189
XspI CTAG 3 cut(s) 12, 1718, 1997
ZraI GACGTC 1 cut(s) 1895
ZrmI AGTACT 1 cut(s) 563
Zsp2I ATGCAT 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.