pycom06g15570

DYW family of nucleic acid deaminases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0014735
Physical Location & Seq
Forward (+)
20079651 .. 20082197
2547 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2547 bp
ATGGCGTTTGTAGTGCCACAAGGCTGCTATTCAATTCATTACCCGGCAATACAATCGGAGGCGGCGTCACACTACAAATTCCGACGTCCAAGTACGCCGTGCTTTCTATCTTTTACTTCAAAGTTTCATAATTTCAGCTCCGGAATCCAATTCCTGAAAGCCCCATCTCGGAAACCGATTCTGGGACGTGGGTTTTCGCGGGAGAGTTCGCTGCTCGAAGTGTCGTCAGCCGGCCAGCTGATTAAGGACTACGTGGAGGACGGGAACTTCGAAGATGCAATCACCATTTACGTCAAAGTTCTTGAATTTGGTCTGCCGGTTTCGGAATTTCGATTCTTTCCCTGTTTGATTAAGGCGTTCGGGGGGCTTTGTGATGCCGGAAAGGCTCGCGAGATTCATGGGCACGTCTTGAAATTAGGAGTTTTGGATGATGTTTACGATGTCAATTCGCTTTTGGGTGTTTACTGGAAGTGCGGGGCTGTTGAGGATGCAATCCAGCTGTTTGAGAAAATGCGTGAGAGAGATTTGGTTTCTTGGAATACTATGATATCTGGGCTTTGCCATTGGGGAGATTATATGGGTTCGTTGAGGATGTTTAGTCGGATGGTTAATGATTACTGGGTGTTGCCGAACCGGGTGGCTTGCCTTTCGGCTCTCTCGTCATGCTCTTCAGTTCAGCGTTCAGTTCATGGAAGGGAAATTCATGGATTTGTTGTGAAAAGGGAGATAGCTACAGATCAGTTTTTGGTTAGTGGATTAATCGATATGTACATGAAATGTAGGGATGTAAAGAATGCGGAATATGTTTTTGGGAGCATTGTTTATGAAGAATCGATCATTGGGAATCCGGTGATATGGAATGTGATGATCTCGGGATATGTTGCGAACGGGCGTTTGCCACAAGCAGTGGAGTTGTTCCTAGAGATGTTAGAAATTGCTTTATCACCAGATACTTCCACAATGGTCGCCGTTATAGGTTTGTGCTCTCAACTGTTGGATTTAGCATTGGGAAGGCAAATCCATAAGTTCTGTTATAGCATTCAATTGAACCATGATGCAAGAGTTGAAACGGCTCTTATGGACATGTATTTTAAATGCGGTGATAGCAAAGCTGGCCTTGAAATCTTTCAAAGATCTCAAAATCGTAACATTGTCATGTGGGGAGCTATCATTTCAAATTTCGCTCAAAGCAGTCGTCCTCATGAGGCATTGAATCTGTTCCATAACTATGTATTAGAATATGGCTTTGTGGATTCTGTCATTCTATTGGCTGTTCTGCGAGCCTGTTCTTCCTTGGCTGTTAGGCCTAGAGGCATGGAAATCCATGGGCTAGTAGTAAAAATGGGGTTTGATTTAGATGTTTTTGTCGGAGGTGCCTTAGTCGATATGTATGCAAAATGCAGAGACATGGAGTCAGCTCAAATTGTCTTTTATAGACTACCCGCTAGAGATTTGGTATCTTGGAATGCCTTGATATCTGGATATACTCAGAATGAGTTTCCAGATGAAGCTTTAAAGGCAATCCTCGACATGCAGTCCGATGGAGTCAAACCCAATGCCGTGACAGTTGCGAGTATGCTGTCGGTTTGTGCTCAGTTGTCAATCATGAAGTCGTGCAAGGAGGTTCACGGTTACCTTTTACGACAAGAATTTGAGTCCAATGTTCTTGTGAGCAATTCCCTTATAACTACCTATGCAAAATGTGGAGACATAAAGAGCTCATGGGCTATATTTGAGAGCATGCCTGAAAGAACTGAAGTATCATGGAATTCAATTCTTTTGGGGTTAGGAATGCACGGCCATGCGGATGAAACATTCGTTTTATTTGAGAAAATGGAAGCAGCAGGAATGAAGCCTGACCATGCGACTTTTACAGCTCTACTGTCTGCTTGCAGCCATGCAGGGAGGGTTGAAGAGGGATTGAAATATTTTAAACGTATGGTCGAGGATTACAAAATTGAACCTCAACTTGAACAGTACACCTGCATGGTTGATCTTTTAGGCCGAGCTGGCCATTTAAAACATGCATACGATATTATTTTGGCAATGCCTTGTACCCCAGATGATCGTATATGGGGATCATTGCTCAGATCATGCAAAATTCACTGTGATGAAAGACTTGCAGAAGTTGTGTCTGATCATATCTTTGAACTCGATCCTACTAGTATTGGTTACCGTACACTTCTTGCAAACTTGTATGAAGAGTATGGGAAATGGAGTGATGCTACTAGGATTAGATCGGAGATCAAAGGCAGGGGGCTCAAGAAGACACCTGGTTGCAGTTGGATTGAAGTCAATAGTAATGTTCATGTATTTATGGCGGGTGATCAGTCTCATAATCAGTTGGAGGAGATATATACTACAGTTGAATGTTTAACAAATGAAATAAGGAAGGCAGGATATATTCCTCAATCACTTACAGTAAGTGTTCGGCATGATGAGGTTAATCATGAAAATCTTTCATCATTTAAAGACGATGATTTGCTGTTTCCGGTTGCAAGTAGAAACCATAGCCGAACAGGTTCAGTGGAATTGATGATTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

849

Amino Acids

95.21

Weight (kDa)

5.86

Isoelectric Point (pI)

44.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 177 - 204 7.6e-06 PPR repeat
PPR_2 PF13041 283 - 328 2.1e-08 PPR repeat family
PPR PF01535 284 - 311 7.8e-07 PPR repeat
PPR_2 PF13041 484 - 531 6.3e-10 PPR repeat family
PPR PF01535 486 - 516 2.2e-06 PPR repeat
PPR_2 PF13041 585 - 632 6.1e-10 PPR repeat family
PPR_long PF17177 600 - 679 1.6e-06 Pentacotripeptide-repeat region of PRORP
PPR PF01535 623 - 650 8.4e-06 PPR repeat
E_motif PF20431 702 - 764 1.2e-14 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014735)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05260
malus_domestica MD06G1174400.v1.1 MD06G1174600.v1.1
prunus_persica Prupe.5G174400_v2.0.a1 Prupe.5G174400_v2.0.a1 Prupe.5G174400_v2.0.a1
pyrus_communis pycom06g15570
rosa_chinensis RchiOBHm_Chr7g0193611
rosa_laevigata RLG00000004256
rosa_multiflora Rmu_sc0042944.1_g000002
rosa_roxburghii Rroxscaffold_3G00261960
rosa_rugosa Rorug07G0011000 Rorug07G0011100
rosa_samantha Rh7AG137600 Rh7BG137800 Rh7CG141400
rosa_wichuraiana Rw7G011720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1685
AarI CACCTGC 1 cut(s) 1991
AatII GACGTC 1 cut(s) 88
Acc36I ACCTGC 1 cut(s) 1991
AccB1I GGYRCC 1 cut(s) 1373
AccII CGCG 2 cut(s) 199, 390
AccIII TCCGGA 1 cut(s) 140
AciI CCGC 8 cut(s) 62, 199, 474, 797, 1098, 1443, 1805, 2321
AclWI GGATC 2 cut(s) 2086, 2150
AcoI YGGCCR 3 cut(s) 232, 1798, 2011
AcsI RAATTY 8 cut(s) 77, 305, 326, 699, 1177, 1649, 1768, 2100
AcuI CTGAAG 2 cut(s) 654, 1776
AcyI GRCGYC 2 cut(s) 65, 85
AfaI GTAC 5 cut(s) 94, 770, 1979, 2056, 2179
AfiI CCNNNNNNNGG 2 cut(s) 168, 182
AflIII ACRYGT 1 cut(s) 1083
AhdI GACNNNNNGTC 2 cut(s) 1411, 1534
AhlI ACTAGT 1 cut(s) 2162
AjiI CACGTC 2 cut(s) 188, 406
AjnI CCWGG 1 cut(s) 2272
AjuI GAANNNNNNNTTGG 5 cut(s) 792, 822, 824, 854, 2523
Alw21I GWGCWC 3 cut(s) 986, 1594, 1721
Alw26I GTCTC 3 cut(s) 1398, 1701, 2337
AlwI GGATC 2 cut(s) 2086, 2150
Ama87I CYCGRG 1 cut(s) 871
Aor13HI TCCGGA 1 cut(s) 140
AoxI GGCC 6 cut(s) 232, 1114, 1304, 1798, 2002, 2011
ApeKI GCWGC 4 cut(s) 24, 211, 1841, 1893
ApoI RAATTY 8 cut(s) 77, 305, 326, 699, 1177, 1649, 1768, 2100
ArsI GACNNNNNNTTYG 4 cut(s) 251, 283, 1180, 1212
AseI ATTAAT 1 cut(s) 758
Asp700I GAANNNNTTC 3 cut(s) 1125, 1217, 2521
AsuC2I CCSGG 2 cut(s) 44, 635
AsuHPI GGTGA 5 cut(s) 274, 862, 936, 1112, 2336
AsuII TTCGAA 1 cut(s) 270
AvaI CYCGRG 1 cut(s) 871
BaeGI GKGCMC 1 cut(s) 405
BaeI ACNNNNGTAYC 2 cut(s) 1743, 1776
BalI TGGCCA 1 cut(s) 2013
BanI GGYRCC 1 cut(s) 1373
BanII GRGCYC 2 cut(s) 1721, 2262
BbsI GAAGAC 1 cut(s) 2273
Bbv12I GWGCWC 3 cut(s) 986, 1594, 1721
BbvI GCAGC 4 cut(s) 11, 198, 1853, 1905
BccI CCATC 3 cut(s) 172, 598, 1535
BceAI ACGGC 5 cut(s) 82, 953, 1086, 1544, 1813
BcgI CGANNNNNNTGC 4 cut(s) 36, 70, 1373, 1407
BciT130I CCWGG 1 cut(s) 2274
BclI TGATCA 2 cut(s) 2137, 2326
BcnI CCSGG 2 cut(s) 44, 635
BcoDI GTCTC 3 cut(s) 1398, 1701, 2337
BcuI ACTAGT 1 cut(s) 2162
BfaI CTAG 6 cut(s) 920, 1308, 1331, 1446, 2163, 2229
BfmI CTRYAG 2 cut(s) 732, 2361
BfuAI ACCTGC 1 cut(s) 1991
BglI GCCNNNNNGGC 2 cut(s) 383, 2010
BglII AGATCT 1 cut(s) 1133
BisI GCNGC 5 cut(s) 25, 63, 212, 1842, 1894
BlsI GCNGC 5 cut(s) 26, 64, 213, 1843, 1895
Bme1390I CCNGG 3 cut(s) 44, 635, 2274
BmeRI GACNNNNNGTC 2 cut(s) 1411, 1534
BmeT110I CYCGRG 1 cut(s) 871
BmgBI CACGTC 2 cut(s) 188, 406
BmiI GGNNCC 1 cut(s) 1375
BmrFI CCNGG 3 cut(s) 44, 635, 2274
BmrI ACTGGG 1 cut(s) 628
BmsI GCATC 5 cut(s) 265, 364, 478, 1045, 2212
BmuI ACTGGG 1 cut(s) 628
BpiI GAAGAC 1 cut(s) 2273
Bpu14I TTCGAA 1 cut(s) 270
BpuEI CTTGAG 1 cut(s) 2246
BpuMI CCSGG 2 cut(s) 44, 635
Bsa29I ATCGAT 2 cut(s) 762, 833
BsaAI YACGTR 1 cut(s) 253
BsaHI GRCGYC 2 cut(s) 65, 85
BsaJI CCNNGG 2 cut(s) 1293, 1324
BsaWI WCCGGW 3 cut(s) 140, 847, 2491
BsaXI ACNNNNNCTCC 2 cut(s) 50, 80
Bsc4I CCNNNNNNNGG 2 cut(s) 168, 182
Bse118I RCCGGY 2 cut(s) 230, 316
Bse1I ACTGG 2 cut(s) 470, 623
Bse3DI GCAATG 2 cut(s) 2052, 2081
BseAI TCCGGA 1 cut(s) 140
BseBI CCWGG 1 cut(s) 2274
BseCI ATCGAT 2 cut(s) 762, 833
BseDI CCNNGG 2 cut(s) 1293, 1324
BseGI GGATG 6 cut(s) 433, 493, 597, 609, 790, 1813
BseLI CCNNNNNNNGG 2 cut(s) 168, 182
BseMI GCAATG 2 cut(s) 2052, 2081
BseMII CTCAG 3 cut(s) 1502, 1607, 2101
BseNI ACTGG 2 cut(s) 470, 623
BseRI GAGGAG 1 cut(s) 2363
BseSI GKGCMC 1 cut(s) 405
BseXI GCAGC 4 cut(s) 11, 198, 1853, 1905
Bsh1236I CGCG 2 cut(s) 199, 390
BshFI GGCC 6 cut(s) 234, 1116, 1306, 1800, 2004, 2013
BshNI GGYRCC 1 cut(s) 1373
BshVI ATCGAT 2 cut(s) 762, 833
BsiHKAI GWGCWC 3 cut(s) 986, 1594, 1721
BsiHKCI CYCGRG 1 cut(s) 871
BsiSI CCGG 8 cut(s) 44, 141, 231, 317, 378, 634, 848, 2492
BslFI GGGAC 1 cut(s) 198
BslI CCNNNNNNNGG 2 cut(s) 168, 182
BsmAI GTCTC 3 cut(s) 1398, 1701, 2337
BsmFI GGGAC 1 cut(s) 198
BsmI GAATGC 4 cut(s) 799, 1038, 1471, 1797
BsnI GGCC 6 cut(s) 234, 1116, 1306, 1800, 2004, 2013
BsoBI CYCGRG 1 cut(s) 871
Bsp119I TTCGAA 1 cut(s) 270
Bsp1286I GDGCHC 5 cut(s) 405, 986, 1594, 1721, 2262
Bsp13I TCCGGA 1 cut(s) 140
Bsp1407I TGTACA 1 cut(s) 768
Bsp19I CCATGG 1 cut(s) 1324
Bsp68I TCGCGA 1 cut(s) 390
BspACI CCGC 8 cut(s) 62, 199, 474, 797, 1098, 1443, 1805, 2321
BspANI GGCC 6 cut(s) 234, 1116, 1306, 1800, 2004, 2013
BspCNI CTCAG 3 cut(s) 1501, 1606, 2100
BspDI ATCGAT 2 cut(s) 762, 833
BspEI TCCGGA 1 cut(s) 140
BspFNI CGCG 2 cut(s) 199, 390
BspHI TCATGA 3 cut(s) 1201, 1605, 2449
BspLI GGNNCC 1 cut(s) 1375
BspMI ACCTGC 1 cut(s) 1991
BspPI GGATC 2 cut(s) 2086, 2150
BspQI GCTCTTC 1 cut(s) 673
BspT104I TTCGAA 1 cut(s) 270
BspT107I GGYRCC 1 cut(s) 1373
BsrDI GCAATG 2 cut(s) 2052, 2081
BsrFI RCCGGY 2 cut(s) 230, 316
BsrGI TGTACA 1 cut(s) 768
BsrI ACTGG 2 cut(s) 470, 623
BssAI RCCGGY 2 cut(s) 230, 316
BssECI CCNNGG 2 cut(s) 1293, 1324
BssNI GRCGYC 2 cut(s) 65, 85
BssT1I CCWWGG 2 cut(s) 1293, 1324
Bst2UI CCWGG 1 cut(s) 2274
Bst4CI ACNGT 9 cut(s) 993, 1567, 1631, 1884, 1977, 2108, 2177, 2365, 2422
Bst6I CTCTTC 3 cut(s) 673, 1908, 2196
BstACI GRCGYC 2 cut(s) 65, 85
BstAUI TGTACA 1 cut(s) 768
BstBAI YACGTR 1 cut(s) 253
BstBI TTCGAA 1 cut(s) 270
BstC8I GCNNGC 9 cut(s) 232, 236, 388, 643, 1114, 1281, 1742, 1891, 2011
BstDEI CTNAG 4 cut(s) 1378, 1488, 1593, 2087
BstDSI CCRYGG 1 cut(s) 1324
BstEII GGTNACC 2 cut(s) 1631, 2171
BstF5I GGATG 6 cut(s) 433, 493, 597, 609, 790, 1813
BstFNI CGCG 2 cut(s) 199, 390
BstMAI GTCTC 3 cut(s) 1398, 1701, 2337
BstMWI GCNNNNNNNGC 5 cut(s) 383, 1104, 1113, 1517, 2010
BstNI CCWGG 1 cut(s) 2274
BstNSI RCATGY 4 cut(s) 1087, 1534, 1744, 2027
BstPI GGTNACC 2 cut(s) 1631, 2171
BstSCI CCNGG 3 cut(s) 42, 633, 2272
BstSFI CTRYAG 2 cut(s) 732, 2361
BstSLI GKGCMC 1 cut(s) 405
BstUI CGCG 2 cut(s) 199, 390
BstV1I GCAGC 4 cut(s) 11, 198, 1853, 1905
BstV2I GAAGAC 1 cut(s) 2273
BstX2I RGATCY 1 cut(s) 1133
BstYI RGATCY 1 cut(s) 1133
Bsu15I ATCGAT 2 cut(s) 762, 833
BsuRI GGCC 6 cut(s) 234, 1116, 1306, 1800, 2004, 2013
BsuTUI ATCGAT 2 cut(s) 762, 833
BtgI CCRYGG 1 cut(s) 1324
BtrI CACGTC 2 cut(s) 188, 406
BtsCI GGATG 6 cut(s) 433, 493, 597, 609, 790, 1813
BtsI GCAGTG 1 cut(s) 912
BtsIMutI CAGTG 3 cut(s) 912, 2104, 2532
BtuMI TCGCGA 1 cut(s) 390
BveI ACCTGC 1 cut(s) 1991
Cac8I GCNNGC 9 cut(s) 232, 236, 388, 643, 1114, 1281, 1742, 1891, 2011
CciI TCATGA 3 cut(s) 1201, 1605, 2449
Cfr10I RCCGGY 2 cut(s) 230, 316
ClaI ATCGAT 2 cut(s) 762, 833
CseI GACGC 1 cut(s) 54
CsiI ACCWGGT 1 cut(s) 2272
Csp6I GTAC 5 cut(s) 93, 769, 1978, 2055, 2178
CviQI GTAC 5 cut(s) 93, 769, 1978, 2055, 2178
DdeI CTNAG 4 cut(s) 1378, 1488, 1593, 2087
DraI TTTAAA 5 cut(s) 1093, 1515, 1933, 2019, 2470
DriI GACNNNNNGTC 2 cut(s) 1411, 1534
EaeI YGGCCR 3 cut(s) 232, 1798, 2011
Eam1104I CTCTTC 3 cut(s) 673, 1908, 2196
Eam1105I GACNNNNNGTC 2 cut(s) 1411, 1534
EarI CTCTTC 3 cut(s) 673, 1908, 2196
Ecl136II GAGCTC 1 cut(s) 1719
Eco130I CCWWGG 2 cut(s) 1293, 1324
Eco147I AGGCCT 1 cut(s) 1306
Eco24I GRGCYC 2 cut(s) 1721, 2262
Eco32I GATATC 2 cut(s) 549, 1476
Eco53kI GAGCTC 1 cut(s) 1719
Eco57I CTGAAG 2 cut(s) 654, 1776
Eco88I CYCGRG 1 cut(s) 871
Eco91I GGTNACC 2 cut(s) 1631, 2171
EcoICRI GAGCTC 1 cut(s) 1719
EcoO65I GGTNACC 2 cut(s) 1631, 2171
EcoRI GAATTC 1 cut(s) 1768
EcoRII CCWGG 1 cut(s) 2272
EcoRV GATATC 2 cut(s) 549, 1476
EcoT14I CCWWGG 2 cut(s) 1293, 1324
EcoT22I ATGCAT 1 cut(s) 2029
EcoT38I GRGCYC 2 cut(s) 1721, 2262
ErhI CCWWGG 2 cut(s) 1293, 1324
FalI AAGNNNNNCTT 2 cut(s) 1101, 1133
FaqI GGGAC 1 cut(s) 198
FauI CCCGC 4 cut(s) 192, 467, 1450, 2314
FbaI TGATCA 2 cut(s) 2137, 2326
Fnu4HI GCNGC 5 cut(s) 25, 63, 212, 1842, 1894
FokI GGATG 6 cut(s) 440, 500, 604, 616, 797, 1820
FriOI GRGCYC 2 cut(s) 1721, 2262
Fsp4HI GCNGC 5 cut(s) 25, 63, 212, 1842, 1894
FspBI CTAG 6 cut(s) 920, 1308, 1331, 1446, 2163, 2229
GluI GCNGC 5 cut(s) 25, 63, 212, 1842, 1894
HaeIII GGCC 6 cut(s) 234, 1116, 1306, 1800, 2004, 2013
HapII CCGG 8 cut(s) 44, 141, 231, 317, 378, 634, 848, 2492
HgaI GACGC 1 cut(s) 54
Hin1I GRCGYC 2 cut(s) 65, 85
HindIII AAGCTT 1 cut(s) 1509
HpaII CCGG 8 cut(s) 44, 141, 231, 317, 378, 634, 848, 2492
HphI GGTGA 5 cut(s) 274, 862, 936, 1112, 2336
Hpy166II GTNNAC 5 cut(s) 436, 463, 1627, 1980, 2180
Hpy8I GTNNAC 5 cut(s) 436, 463, 1627, 1980, 2180
Hpy99I CGWCG 1 cut(s) 87
HpyAV CCTTC 3 cut(s) 687, 1005, 2386
HpyCH4III ACNGT 9 cut(s) 993, 1567, 1631, 1884, 1977, 2108, 2177, 2365, 2422
HpyCH4IV ACGT 6 cut(s) 85, 187, 252, 291, 405, 1936
HpyF10VI GCNNNNNNNGC 5 cut(s) 383, 1104, 1113, 1517, 2010
HpyF3I CTNAG 4 cut(s) 1378, 1488, 1593, 2087
HpySE526I ACGT 6 cut(s) 85, 187, 252, 291, 405, 1936
Hsp92I GRCGYC 2 cut(s) 65, 85
Kpn2I TCCGGA 1 cut(s) 140
KroI GCCGGC 1 cut(s) 230
KroNI GCCGGC 1 cut(s) 232
Ksp22I TGATCA 2 cut(s) 2137, 2326
LguI GCTCTTC 1 cut(s) 673
LmnI GCTCC 3 cut(s) 143, 813, 1163
Lsp1109I GCAGC 4 cut(s) 11, 198, 1853, 1905
LweI GCATC 5 cut(s) 265, 364, 478, 1045, 2212
MabI ACCWGGT 1 cut(s) 2272
MaeI CTAG 6 cut(s) 920, 1308, 1331, 1446, 2163, 2229
MaeII ACGT 6 cut(s) 85, 187, 252, 291, 405, 1936
MaeIII GTNAC 5 cut(s) 66, 1145, 1561, 1631, 2171
MboII GAAGA 7 cut(s) 284, 660, 839, 1281, 1925, 2213, 2278
MfeI CAATTG 1 cut(s) 1043
MflI RGATCY 1 cut(s) 1133
MhlI GDGCHC 5 cut(s) 405, 986, 1594, 1721, 2262
MlsI TGGCCA 1 cut(s) 2013
MluNI TGGCCA 1 cut(s) 2013
MlyI GAGTC 3 cut(s) 1421, 1554, 1664
MmeI TCCRAC 6 cut(s) 106, 581, 975, 1348, 2264, 2325
Mox20I TGGCCA 1 cut(s) 2013
Mph1103I ATGCAT 1 cut(s) 2029
MroI TCCGGA 1 cut(s) 140
MroNI GCCGGC 1 cut(s) 230
MroXI GAANNNNTTC 3 cut(s) 1125, 1217, 2521
MscI TGGCCA 1 cut(s) 2013
MslI CAYNNNNRTG 6 cut(s) 1154, 1227, 1800, 1806, 1985, 2109
Msp20I TGGCCA 1 cut(s) 2013
MspA1I CMGCKG 2 cut(s) 238, 499
MspI CCGG 8 cut(s) 44, 141, 231, 317, 378, 634, 848, 2492
MspR9I CCNGG 3 cut(s) 44, 635, 2274
MunI CAATTG 1 cut(s) 1043
Mva1269I GAATGC 4 cut(s) 799, 1038, 1471, 1797
MvaI CCWGG 1 cut(s) 2274
MvnI CGCG 2 cut(s) 199, 390
MwoI GCNNNNNNNGC 5 cut(s) 383, 1104, 1113, 1517, 2010
NaeI GCCGGC 1 cut(s) 232
NciI CCSGG 2 cut(s) 44, 635
NcoI CCATGG 1 cut(s) 1324
NgoMIV GCCGGC 1 cut(s) 230
NlaIV GGNNCC 1 cut(s) 1375
NmeAIII GCCGAG 1 cut(s) 2030
NmuCI GTSAC 2 cut(s) 66, 1561
NruI TCGCGA 1 cut(s) 390
NsiI ATGCAT 1 cut(s) 2029
NspI RCATGY 4 cut(s) 1087, 1534, 1744, 2027
NspV TTCGAA 1 cut(s) 270
PaeI GCATGC 1 cut(s) 1744
PagI TCATGA 3 cut(s) 1201, 1605, 2449
PaqCI CACCTGC 1 cut(s) 1991
PceI AGGCCT 1 cut(s) 1306
PciI ACATGT 1 cut(s) 1083
PciSI GCTCTTC 1 cut(s) 673
PcsI WCGNNNNNNNCGW 3 cut(s) 62, 267, 656
PctI GAATGC 4 cut(s) 799, 1038, 1471, 1797
PdiI GCCGGC 1 cut(s) 232
PdmI GAANNNNTTC 3 cut(s) 1125, 1217, 2521
PfeI GAWTC 8 cut(s) 144, 178, 333, 394, 830, 844, 1213, 1253
PkrI GCNGC 5 cut(s) 26, 64, 213, 1843, 1895
PleI GAGTC 3 cut(s) 1420, 1553, 1663
PpsI GAGTC 3 cut(s) 1420, 1553, 1663
Ppu21I YACGTR 1 cut(s) 253
PscI ACATGT 1 cut(s) 1083
PshBI ATTAAT 1 cut(s) 758
PsiI TTATAA 1 cut(s) 1685
Psp124BI GAGCTC 1 cut(s) 1721
Psp6I CCWGG 1 cut(s) 2272
PspEI GGTNACC 2 cut(s) 1631, 2171
PspGI CCWGG 1 cut(s) 2272
PspN4I GGNNCC 1 cut(s) 1375
PsuI RGATCY 1 cut(s) 1133
PvuII CAGCTG 2 cut(s) 238, 499
RruI TCGCGA 1 cut(s) 390
RsaI GTAC 5 cut(s) 94, 770, 1979, 2056, 2179
RsaNI GTAC 5 cut(s) 93, 769, 1978, 2055, 2178
RseI CAYNNNNRTG 6 cut(s) 1154, 1227, 1800, 1806, 1985, 2109
SacI GAGCTC 1 cut(s) 1721
SapI GCTCTTC 1 cut(s) 673
SatI GCNGC 5 cut(s) 25, 63, 212, 1842, 1894
SchI GAGTC 3 cut(s) 1421, 1554, 1664
ScrFI CCNGG 3 cut(s) 44, 635, 2274
SduI GDGCHC 5 cut(s) 405, 986, 1594, 1721, 2262
SexAI ACCWGGT 1 cut(s) 2272
SfaNI GCATC 5 cut(s) 265, 364, 478, 1045, 2212
SfcI CTRYAG 2 cut(s) 732, 2361
SfiI GGCCNNNNNGGCC 1 cut(s) 2010
SfuI TTCGAA 1 cut(s) 270
SmiMI CAYNNNNRTG 6 cut(s) 1154, 1227, 1800, 1806, 1985, 2109
SmlI CTYRAG 1 cut(s) 2261
SmoI CTYRAG 1 cut(s) 2261
SpeI ACTAGT 1 cut(s) 2162
SphI GCATGC 1 cut(s) 1744
SseBI AGGCCT 1 cut(s) 1306
SsiI CCGC 8 cut(s) 62, 199, 474, 797, 1098, 1443, 1805, 2321
SspI AATATT 1 cut(s) 1928
SspMI CTAG 6 cut(s) 920, 1308, 1331, 1446, 2163, 2229
SstI GAGCTC 1 cut(s) 1721
StuI AGGCCT 1 cut(s) 1306
StyD4I CCNGG 3 cut(s) 42, 633, 2272
StyI CCWWGG 2 cut(s) 1293, 1324
TaaI ACNGT 9 cut(s) 993, 1567, 1631, 1884, 1977, 2108, 2177, 2365, 2422
TaiI ACGT 6 cut(s) 88, 190, 255, 294, 408, 1939
TaqI TCGA 9 cut(s) 216, 270, 331, 762, 833, 1383, 1527, 1944, 2154
TatI WGTACW 2 cut(s) 768, 1977
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 8 cut(s) 144, 178, 333, 394, 830, 844, 1213, 1253
TscAI CASTG 3 cut(s) 912, 2111, 2532
TseFI GTSAC 2 cut(s) 66, 1561
TseI GCWGC 4 cut(s) 24, 211, 1841, 1893
Tsp45I GTSAC 2 cut(s) 66, 1561
TspRI CASTG 3 cut(s) 912, 2111, 2532
VspI ATTAAT 1 cut(s) 758
XapI RAATTY 8 cut(s) 77, 305, 326, 699, 1177, 1649, 1768, 2100
XceI RCATGY 4 cut(s) 1087, 1534, 1744, 2027
XmnI GAANNNNTTC 3 cut(s) 1125, 1217, 2521
XspI CTAG 6 cut(s) 920, 1308, 1331, 1446, 2163, 2229
ZraI GACGTC 1 cut(s) 86
Zsp2I ATGCAT 1 cut(s) 2029
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.