Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr6

Orthogroup ID

OG0012512

Physical Location & Seq

Genomic Coordinates Reverse (-)

20132506 .. 20135423

Gene Structure 2918 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom06g15630.7

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1116 bp

ATGGCCATCCGAGGCGTCGATTTCAAGTGGTACGATGGGTTCTTCTTGTCGATGCTCGCGACGAGCGTAGTGATTGTTGGAATTAATTGGAAGCGGTACCATACTTGTGCATACCCGTTGCACATATGGATCGTGGTTGATTACACTACTGTGTTTGTTTTTCGGATGTTGATGGATTTTGGGTGGCAGCAAAGATATGCTCGTTTCTGCGGAAGAGTAGTGGTCCTTTCAATTCTTTCTCTGCTGCTTTATCCATTTCTTTGGACTTGGACACTGCTTGGTACACTGTGGTTTCACAAATCGAAGGACTGTTTGCCTGAAAGTGGTCAGAAGTGGGGTTTTCTCATTTGGTTACTTTTTAGCTACAGTGCTCTACTTTGCATTGCTTGCATGTCAATTGGGAAGTGGTTGACACGAAGGGAGGCACACTTACTGCGAGCTCAGCAAGGGATTCCTGTGTCAGAATTTGGGGTTTTGGTTGATATGATTCGGGTGCCAGATTGGGCATTTGAAGCTGCCGGTCAAGAAACAAGAGGGATGGGCCAAGATGCTGCTGCATTCCAACCTGGACTTTACTTGACTCAAGCACAGAGAGAAGCAGTTGAAGCACTCATTCAGGAGCTTCCAAAGTTCAGACTGAAGGCTGTTCCAACTGATTGCAGCGAATGTCCAATCTGCTTGGAAGAGTTTCATGTAGGCAATGAGGTTCGCGGCCTGCCTTGTGCTCACAATTTCCATGTAGAATGCATCGATGAGTGGCTTCGACTGAATGTGAAATGTCCCAGATGCCGTTGCTCAGTCTTCCCAAATCTCGACCTCAGTGCTATATCCAATCTCCGTCCTGACTCTGAGCGTTCACCTCTCAGTGTGTTGACAACCAACCGGTATGTGAGAACCCAACCTGATAGTCAGAGGCATTTGTTGAGGTTGCAGGGAGCATTCCGCCCAGTCCGTACAGAAAATGCAGGTGCAGGCAATGAGGCAGACGCTGCTTCGGAAACTGCTGAGGAAGGGGGTATTGCTCATTCATCTACTCGGGATCTTTCAAGCCCAGGGGCGGGACCTTCCATTGAACATATCGTCGTGGTCGAATCCGCCTCCCGGCAACAACAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009743 GO:0009756 GO:0009987 GO:0010033 GO:0010182 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0023052 GO:0030163 GO:0032446 GO:0036211 GO:0042221 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0050789 GO:0050794 GO:0050896 GO:0051603 GO:0051716 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0070887 GO:0071310 GO:0071322 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

372

Amino Acids

41.97

Weight (kDa)

6.79

Isoelectric Point (pI)

44.9

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012512)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G47990 AT3G47990 AT3G47990 AT3G47990 AT3G47990
fragaria_vesca	FvH4_5g05210 FvH4_5g05210
malus_domestica	MD06G1175600.v1.1
prunus_persica	Prupe.5G174900_v2.0.a1
pyrus_communis	pycom06g15630 pycom14g15130
rosa_chinensis	RchiOBHm_Chr7g0193701
rosa_laevigata	RLG00000004250
rosa_multiflora	Rmu_sc0001436.1_g000009
rosa_roxburghii	Rroxscaffold_3G00261890
rosa_rugosa	Rorug07G0011400
rosa_samantha	Rh7AG138200 Rh7DG139500
rosa_wichuraiana	Rw7G011770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	956
Acc36I	ACCTGC	1 cut(s)	956
Acc65I	GGTACC	1 cut(s)	96
AccB1I	GGYRCC	2 cut(s)	96, 493
AccII	CGCG	2 cut(s)	59, 711
AciI	CCGC	6 cut(s)	94, 210, 711, 943, 1058, 1095
AclWI	GGATC	2 cut(s)	137, 1047
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	1 cut(s)	464
AcuI	CTGAAG	1 cut(s)	659
AcyI	GRCGYC	1 cut(s)	15
AfaI	GTAC	4 cut(s)	32, 98, 283, 955
AfiI	CCNNNNNNNGG	4 cut(s)	323, 1057, 1058, 1101
AgeI	ACCGGT	1 cut(s)	882
AgsI	TTSAA	6 cut(s)	25, 231, 512, 605, 1047, 1073
AjnI	CCWGG	2 cut(s)	565, 1051
AleI	CACNNNNGTG	1 cut(s)	149
AluBI	AGCT	4 cut(s)	363, 440, 515, 622
AluI	AGCT	4 cut(s)	363, 440, 515, 622
Alw21I	GWGCWC	3 cut(s)	373, 442, 727
AlwI	GGATC	2 cut(s)	137, 1047
AlwNI	CAGNNNCTG	1 cut(s)	989
Ama87I	CYCGRG	1 cut(s)	1035
AoxI	GGCC	3 cut(s)	3, 541, 712
ApeKI	GCWGC	7 cut(s)	187, 244, 515, 551, 554, 660, 989
ApoI	RAATTY	1 cut(s)	464
AseI	ATTAAT	1 cut(s)	84
AsiGI	ACCGGT	1 cut(s)	882
Asp700I	GAANNNNTTC	1 cut(s)	687
Asp718I	GGTACC	1 cut(s)	96
AspS9I	GGNCC	3 cut(s)	223, 541, 1061
AsuC2I	CCSGG	1 cut(s)	1102
AsuHPI	GGTGA	1 cut(s)	849
AvaI	CYCGRG	1 cut(s)	1035
AvaII	GGWCC	2 cut(s)	223, 1061
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	2 cut(s)	96, 493
BanII	GRGCYC	1 cut(s)	442
BbsI	GAAGAC	1 cut(s)	793
Bbv12I	GWGCWC	3 cut(s)	373, 442, 727
BbvCI	CCTCAGC	1 cut(s)	1005
BbvI	GCAGC	7 cut(s)	199, 231, 502, 538, 541, 672, 976
BccI	CCATC	4 cut(s)	14, 29, 166, 532
BceAI	ACGGC	1 cut(s)	774
BcgI	CGANNNNNNTGC	2 cut(s)	803, 837
BciT130I	CCWGG	2 cut(s)	567, 1053
BcnI	CCSGG	1 cut(s)	1102
BfmI	CTRYAG	1 cut(s)	364
BfuAI	ACCTGC	1 cut(s)	956
BisI	GCNGC	8 cut(s)	188, 245, 516, 552, 555, 661, 712, 990
BlpI	GCTNAGC	1 cut(s)	441
BlsI	GCNGC	8 cut(s)	189, 246, 517, 553, 556, 662, 713, 991
Bme1390I	CCNGG	3 cut(s)	567, 1053, 1102
Bme18I	GGWCC	2 cut(s)	223, 1061
BmeT110I	CYCGRG	1 cut(s)	1035
BmgT120I	GGNCC	3 cut(s)	223, 541, 1061
BmiI	GGNNCC	3 cut(s)	98, 495, 1062
BmrFI	CCNGG	3 cut(s)	567, 1053, 1102
BmrI	ACTGGG	1 cut(s)	941
BmsI	GCATC	4 cut(s)	42, 538, 756, 776
BmuI	ACTGGG	1 cut(s)	941
BpiI	GAAGAC	1 cut(s)	793
Bpu10I	CCTNAGC	1 cut(s)	1005
Bpu1102I	GCTNAGC	1 cut(s)	441
BpuEI	CTTGAG	1 cut(s)	567
BpuMI	CCSGG	1 cut(s)	1102
Bsa29I	ATCGAT	1 cut(s)	750
BsaHI	GRCGYC	1 cut(s)	15
BsaJI	CCNNGG	3 cut(s)	10, 1051, 1052
BsaWI	WCCGGW	1 cut(s)	882
Bsc4I	CCNNNNNNNGG	4 cut(s)	323, 1057, 1058, 1101
Bse118I	RCCGGY	2 cut(s)	518, 882
Bse1I	ACTGG	1 cut(s)	947
Bse3DI	GCAATG	3 cut(s)	381, 706, 982
BseBI	CCWGG	2 cut(s)	567, 1053
BseCI	ATCGAT	1 cut(s)	750
BseDI	CCNNGG	3 cut(s)	10, 1051, 1052
BseGI	GGATG	3 cut(s)	6, 171, 543
BseLI	CCNNNNNNNGG	4 cut(s)	323, 1057, 1058, 1101
BseMI	GCAATG	3 cut(s)	381, 706, 982
BseMII	CTCAG	6 cut(s)	455, 810, 832, 840, 877, 996
BseNI	ACTGG	1 cut(s)	947
BseXI	GCAGC	7 cut(s)	199, 231, 502, 538, 541, 672, 976
BsgI	GTGCAG	1 cut(s)	990
Bsh1236I	CGCG	2 cut(s)	59, 711
BshFI	GGCC	3 cut(s)	5, 543, 714
BshNI	GGYRCC	2 cut(s)	96, 493
BshTI	ACCGGT	1 cut(s)	882
BshVI	ATCGAT	1 cut(s)	750
BsiHKAI	GWGCWC	3 cut(s)	373, 442, 727
BsiHKCI	CYCGRG	1 cut(s)	1035
BsiSI	CCGG	3 cut(s)	519, 883, 1102
BslFI	GGGAC	2 cut(s)	765, 1074
BslI	CCNNNNNNNGG	4 cut(s)	323, 1057, 1058, 1101
BsmFI	GGGAC	2 cut(s)	765, 1074
BsmI	GAATGC	3 cut(s)	557, 749, 938
BsnI	GGCC	3 cut(s)	5, 543, 714
BsoBI	CYCGRG	1 cut(s)	1035
Bsp1286I	GDGCHC	3 cut(s)	373, 442, 727
Bsp143I	GATC	2 cut(s)	129, 1039
Bsp1720I	GCTNAGC	1 cut(s)	441
Bsp68I	TCGCGA	1 cut(s)	59
BspACI	CCGC	6 cut(s)	94, 210, 711, 943, 1058, 1095
BspANI	GGCC	3 cut(s)	5, 543, 714
BspCNI	CTCAG	6 cut(s)	454, 809, 831, 841, 876, 997
BspDI	ATCGAT	1 cut(s)	750
BspFNI	CGCG	2 cut(s)	59, 711
BspLI	GGNNCC	3 cut(s)	98, 495, 1062
BspMI	ACCTGC	1 cut(s)	956
BspPI	GGATC	2 cut(s)	137, 1047
BspT107I	GGYRCC	2 cut(s)	96, 493
BsrDI	GCAATG	3 cut(s)	381, 706, 982
BsrFI	RCCGGY	2 cut(s)	518, 882
BsrI	ACTGG	1 cut(s)	947
BssAI	RCCGGY	2 cut(s)	518, 882
BssECI	CCNNGG	3 cut(s)	10, 1051, 1052
BssMI	GATC	2 cut(s)	129, 1039
BssNI	GRCGYC	1 cut(s)	15
Bst2UI	CCWGG	2 cut(s)	567, 1053
Bst4CI	ACNGT	4 cut(s)	151, 288, 311, 368
Bst6I	CTCTTC	2 cut(s)	208, 678
BstACI	GRCGYC	1 cut(s)	15
BstAPI	GCANNNNNTGC	2 cut(s)	387, 989
BstC8I	GCNNGC	5 cut(s)	57, 388, 438, 716, 973
BstDEI	CTNAG	6 cut(s)	441, 796, 818, 849, 863, 1005
BstF5I	GGATG	3 cut(s)	6, 171, 543
BstFNI	CGCG	2 cut(s)	59, 711
BstKTI	GATC	2 cut(s)	132, 1042
BstMBI	GATC	2 cut(s)	129, 1039
BstMWI	GCNNNNNNNGC	5 cut(s)	387, 442, 512, 605, 989
BstNI	CCWGG	2 cut(s)	567, 1053
BstNSI	RCATGY	1 cut(s)	394
BstSCI	CCNGG	3 cut(s)	565, 1051, 1100
BstSFI	CTRYAG	1 cut(s)	364
BstUI	CGCG	2 cut(s)	59, 711
BstV1I	GCAGC	7 cut(s)	199, 231, 502, 538, 541, 672, 976
BstV2I	GAAGAC	1 cut(s)	793
BstX2I	RGATCY	1 cut(s)	1039
BstXI	CCANNNNNNTGG	1 cut(s)	261
BstYI	RGATCY	1 cut(s)	1039
Bsu15I	ATCGAT	1 cut(s)	750
BsuRI	GGCC	3 cut(s)	5, 543, 714
BsuTUI	ATCGAT	1 cut(s)	750
BtsCI	GGATG	3 cut(s)	6, 171, 543
BtsI	GCAGTG	1 cut(s)	272
BtsIMutI	CAGTG	5 cut(s)	272, 284, 373, 826, 871
BtuMI	TCGCGA	1 cut(s)	59
BveI	ACCTGC	1 cut(s)	956
Cac8I	GCNNGC	5 cut(s)	57, 388, 438, 716, 973
CaiI	CAGNNNCTG	1 cut(s)	989
Cfr10I	RCCGGY	2 cut(s)	518, 882
Cfr13I	GGNCC	3 cut(s)	223, 541, 1061
ClaI	ATCGAT	1 cut(s)	750
CseI	GACGC	2 cut(s)	4, 995
Csp6I	GTAC	4 cut(s)	31, 97, 282, 954
CspAI	ACCGGT	1 cut(s)	882
CviAII	CATG	3 cut(s)	391, 692, 737
CviQI	GTAC	4 cut(s)	31, 97, 282, 954
DdeI	CTNAG	6 cut(s)	441, 796, 818, 849, 863, 1005
DpnI	GATC	2 cut(s)	131, 1041
DpnII	GATC	2 cut(s)	129, 1039
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	2 cut(s)	208, 678
EarI	CTCTTC	2 cut(s)	208, 678
EciI	GGCGGA	2 cut(s)	932, 1084
Ecl136II	GAGCTC	1 cut(s)	440
Eco24I	GRGCYC	1 cut(s)	442
Eco47I	GGWCC	2 cut(s)	223, 1061
Eco53kI	GAGCTC	1 cut(s)	440
Eco57I	CTGAAG	1 cut(s)	659
Eco88I	CYCGRG	1 cut(s)	1035
EcoICRI	GAGCTC	1 cut(s)	440
EcoO109I	RGGNCCY	1 cut(s)	1061
EcoRII	CCWGG	2 cut(s)	565, 1051
EcoT22I	ATGCAT	1 cut(s)	749
EcoT38I	GRGCYC	1 cut(s)	442
FaeI	CATG	3 cut(s)	394, 695, 740
FaqI	GGGAC	2 cut(s)	765, 1074
FatI	CATG	3 cut(s)	390, 691, 736
FauI	CCCGC	1 cut(s)	1051
FauNDI	CATATG	1 cut(s)	125
Fnu4HI	GCNGC	8 cut(s)	188, 245, 516, 552, 555, 661, 712, 990
FokI	GGATG	2 cut(s)	178, 550
FriOI	GRGCYC	1 cut(s)	442
Fsp4HI	GCNGC	8 cut(s)	188, 245, 516, 552, 555, 661, 712, 990
GluI	GCNGC	8 cut(s)	188, 245, 516, 552, 555, 661, 712, 990
HaeIII	GGCC	3 cut(s)	5, 543, 714
HapII	CCGG	3 cut(s)	519, 883, 1102
HgaI	GACGC	2 cut(s)	4, 995
Hin1I	GRCGYC	1 cut(s)	15
Hin1II	CATG	3 cut(s)	394, 695, 740
HincII	GTYRAC	2 cut(s)	411, 873
HindII	GTYRAC	2 cut(s)	411, 873
HinfI	GANTC	5 cut(s)	451, 487, 580, 845, 1091
HpaII	CCGG	3 cut(s)	519, 883, 1102
HphI	GGTGA	1 cut(s)	849
Hpy166II	GTNNAC	4 cut(s)	284, 411, 857, 873
Hpy188I	TCNGA	8 cut(s)	11, 165, 330, 463, 635, 850, 912, 997
Hpy188III	TCNNGA	6 cut(s)	58, 524, 617, 812, 842, 1037
Hpy8I	GTNNAC	4 cut(s)	284, 411, 857, 873
Hpy99I	CGWCG	3 cut(s)	20, 64, 1085
HpyAV	CCTTC	5 cut(s)	298, 411, 634, 1004, 1074
HpyCH4III	ACNGT	4 cut(s)	151, 288, 311, 368
HpyF10VI	GCNNNNNNNGC	5 cut(s)	387, 442, 512, 605, 989
HpyF3I	CTNAG	6 cut(s)	441, 796, 818, 849, 863, 1005
Hsp92I	GRCGYC	1 cut(s)	15
Hsp92II	CATG	3 cut(s)	394, 695, 740
KpnI	GGTACC	1 cut(s)	100
Kzo9I	GATC	2 cut(s)	129, 1039
LmnI	GCTCC	2 cut(s)	619, 935
Lsp1109I	GCAGC	7 cut(s)	199, 231, 502, 538, 541, 672, 976
LweI	GCATC	4 cut(s)	42, 538, 756, 776
MaeIII	GTNAC	1 cut(s)	351
MalI	GATC	2 cut(s)	131, 1041
MboI	GATC	2 cut(s)	129, 1039
MboII	GAAGA	4 cut(s)	34, 225, 695, 793
MfeI	CAATTG	1 cut(s)	396
MflI	RGATCY	1 cut(s)	1039
MhlI	GDGCHC	3 cut(s)	373, 442, 727
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	6 cut(s)	81, 85, 231, 396, 464, 730
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	2 cut(s)	574, 839
MmeI	TCCRAC	3 cut(s)	58, 586, 674
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	1 cut(s)	749
MroXI	GAANNNNTTC	1 cut(s)	687
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	1 cut(s)	84
MslI	CAYNNNNRTG	2 cut(s)	105, 149
Msp20I	TGGCCA	1 cut(s)	5
MspI	CCGG	3 cut(s)	519, 883, 1102
MspR9I	CCNGG	3 cut(s)	567, 1053, 1102
MunI	CAATTG	1 cut(s)	396
Mva1269I	GAATGC	3 cut(s)	557, 749, 938
MvaI	CCWGG	2 cut(s)	567, 1053
MvnI	CGCG	2 cut(s)	59, 711
MwoI	GCNNNNNNNGC	5 cut(s)	387, 442, 512, 605, 989
NciI	CCSGG	1 cut(s)	1102
NdeI	CATATG	1 cut(s)	125
NdeII	GATC	2 cut(s)	129, 1039
NlaIII	CATG	3 cut(s)	394, 695, 740
NlaIV	GGNNCC	3 cut(s)	98, 495, 1062
NruI	TCGCGA	1 cut(s)	59
NsiI	ATGCAT	1 cut(s)	749
NspI	RCATGY	1 cut(s)	394
OliI	CACNNNNGTG	1 cut(s)	149
PaqCI	CACCTGC	1 cut(s)	956
PasI	CCCWGGG	1 cut(s)	1052
PcsI	WCGNNNNNNNCGW	3 cut(s)	56, 63, 1086
PctI	GAATGC	3 cut(s)	557, 749, 938
PdmI	GAANNNNTTC	1 cut(s)	687
PfeI	GAWTC	3 cut(s)	451, 487, 1091
PinAI	ACCGGT	1 cut(s)	882
PkrI	GCNGC	8 cut(s)	189, 246, 517, 553, 556, 662, 713, 991
PleI	GAGTC	2 cut(s)	574, 839
PpsI	GAGTC	2 cut(s)	574, 839
PpuMI	RGGWCCY	1 cut(s)	1061
PshBI	ATTAAT	1 cut(s)	84
Psp124BI	GAGCTC	1 cut(s)	442
Psp5II	RGGWCCY	1 cut(s)	1061
Psp6I	CCWGG	2 cut(s)	565, 1051
PspGI	CCWGG	2 cut(s)	565, 1051
PspN4I	GGNNCC	3 cut(s)	98, 495, 1062
PspPI	GGNCC	3 cut(s)	223, 541, 1061
PspPPI	RGGWCCY	1 cut(s)	1061
PsrI	GAACNNNNNNTAC	2 cut(s)	23, 55
PstNI	CAGNNNCTG	1 cut(s)	989
PsuI	RGATCY	1 cut(s)	1039
RruI	TCGCGA	1 cut(s)	59
RsaI	GTAC	4 cut(s)	32, 98, 283, 955
RsaNI	GTAC	4 cut(s)	31, 97, 282, 954
RseI	CAYNNNNRTG	2 cut(s)	105, 149
SacI	GAGCTC	1 cut(s)	442
SaqAI	TTAA	1 cut(s)	84
SatI	GCNGC	8 cut(s)	188, 245, 516, 552, 555, 661, 712, 990
Sau3AI	GATC	2 cut(s)	129, 1039
Sau96I	GGNCC	3 cut(s)	223, 541, 1061
SchI	GAGTC	2 cut(s)	574, 839
ScrFI	CCNGG	3 cut(s)	567, 1053, 1102
SduI	GDGCHC	3 cut(s)	373, 442, 727
SfaNI	GCATC	4 cut(s)	42, 538, 756, 776
SfcI	CTRYAG	1 cut(s)	364
SinI	GGWCC	2 cut(s)	223, 1061
SmiMI	CAYNNNNRTG	2 cut(s)	105, 149
SmlI	CTYRAG	1 cut(s)	582
SmoI	CTYRAG	1 cut(s)	582
Sse9I	AATT	6 cut(s)	81, 85, 231, 396, 464, 730
SsiI	CCGC	6 cut(s)	94, 210, 711, 943, 1058, 1095
SstI	GAGCTC	1 cut(s)	442
StyD4I	CCNGG	3 cut(s)	565, 1051, 1100
TaaI	ACNGT	4 cut(s)	151, 288, 311, 368
TaqI	TCGA	7 cut(s)	18, 50, 302, 750, 763, 813, 1089
TasI	AATT	6 cut(s)	81, 85, 231, 396, 464, 730
TauI	GCSGC	1 cut(s)	714
TfiI	GAWTC	3 cut(s)	451, 487, 1091
Tru1I	TTAA	1 cut(s)	84
Tru9I	TTAA	1 cut(s)	84
TscAI	CASTG	5 cut(s)	279, 291, 373, 826, 871
TseI	GCWGC	7 cut(s)	187, 244, 515, 551, 554, 660, 989
TspDTI	ATGAA	2 cut(s)	680, 1017
TspGWI	ACGGA	2 cut(s)	827, 941
TspRI	CASTG	5 cut(s)	279, 291, 373, 826, 871
VpaK11BI	GGWCC	2 cut(s)	223, 1061
VspI	ATTAAT	1 cut(s)	84
XapI	RAATTY	1 cut(s)	464
XceI	RCATGY	1 cut(s)	394
XmnI	GAANNNNTTC	1 cut(s)	687
Zsp2I	ATGCAT	1 cut(s)	749

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.