pycom06g15750

Belongs to the spermidine spermine synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0012672
Physical Location & Seq
Forward (+)
20257546 .. 20258634
1089 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 486 bp
ATGTACAATTGTGTATTTTCTGTTGTTTCATTAATTTGGTTACAGTCATCAGAGCATGGCAAGGTAGCCATCTTGGATGGTTTTCTTCAACTCACAGAGAGGGATGAATTTGCTTACCAGGAAATGCTCTCTCACCTCCCACTTTGCTCTATCCCAAACCCAAGGAAGGTCTTGCTTGTTGGAGGCGGAGATGGAGGCATTTTGAGGGAAATATCGCGTCACTCTTCTGTTGAAGAAATCGATATATGCGAAATTGACACAATGGTGATAGATGTTTATAAGCGCTTTTTTCCGGACATAGCTGTGGGATATAAGGATCCCAGAGTGCAAGTACACATAGATGATGGTATGGTGCTACTGCAAAAGAACTTTCTGATAAGGACTTCCTGGAATCAGTTGCAAGGGCTCTTCGCCCCGGCGGTGTGCTGTCTACCCCGGCGGACAGCTTCTGGAGCGAAGACTTTGTTAACTTTGCCGATACCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00330 ko00410 ko00480 ko01100 map00270 map00330 map00410 map00480 map01100
Pfam Domains
Protein Families
Spermine_synt_N Spermine_synth

Protein Analysis

162

Amino Acids

18.06

Weight (kDa)

5.76

Isoelectric Point (pI)

48.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spermine_synth PF01564 36 - 122 6.3e-36 Spermine/spermidine synthase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 279
AccI GTMKAC 1 cut(s) 430
AccII CGCG 1 cut(s) 217
AccIII TCCGGA 1 cut(s) 292
AciI CCGC 3 cut(s) 186, 419, 439
AclWI GGATC 2 cut(s) 311, 324
AcsI RAATTY 1 cut(s) 107
AfaI GTAC 2 cut(s) 5, 333
AfeI AGCGCT 1 cut(s) 284
AfiI CCNNNNNNNGG 1 cut(s) 166
AgsI TTSAA 2 cut(s) 89, 233
AjnI CCWGG 2 cut(s) 117, 386
AleI CACNNNNGTG 1 cut(s) 263
AluBI AGCT 2 cut(s) 302, 446
AluI AGCT 2 cut(s) 302, 446
AlwI GGATC 2 cut(s) 311, 324
AlwNI CAGNNNCTG 1 cut(s) 449
Aor13HI TCCGGA 1 cut(s) 292
Aor51HI AGCGCT 1 cut(s) 284
ApoI RAATTY 1 cut(s) 107
AseI ATTAAT 1 cut(s) 32
AspLEI GCGC 1 cut(s) 285
AsuC2I CCSGG 2 cut(s) 416, 436
AsuHPI GGTGA 2 cut(s) 125, 277
BamHI GGATCC 1 cut(s) 316
BanII GRGCYC 1 cut(s) 408
BbsI GAAGAC 1 cut(s) 464
BccI CCATC 4 cut(s) 71, 77, 185, 338
BciT130I CCWGG 2 cut(s) 119, 388
BcnI CCSGG 2 cut(s) 416, 436
BfoI RGCGCY 1 cut(s) 286
Bme1390I CCNGG 4 cut(s) 119, 388, 416, 436
BmiI GGNNCC 1 cut(s) 318
BmrFI CCNGG 4 cut(s) 119, 388, 416, 436
BpiI GAAGAC 1 cut(s) 464
BpmI CTGGAG 1 cut(s) 471
BpuMI CCSGG 2 cut(s) 416, 436
Bsa29I ATCGAT 1 cut(s) 240
BsaJI CCNNGG 3 cut(s) 161, 414, 434
BsaWI WCCGGW 1 cut(s) 292
Bsc4I CCNNNNNNNGG 1 cut(s) 166
BseAI TCCGGA 1 cut(s) 292
BseBI CCWGG 2 cut(s) 119, 388
BseCI ATCGAT 1 cut(s) 240
BseDI CCNNGG 3 cut(s) 161, 414, 434
BseGI GGATG 2 cut(s) 82, 109
BseLI CCNNNNNNNGG 1 cut(s) 166
Bsh1236I CGCG 1 cut(s) 217
BshVI ATCGAT 1 cut(s) 240
BsiSI CCGG 3 cut(s) 293, 416, 436
BslI CCNNNNNNNGG 1 cut(s) 166
Bsp1286I GDGCHC 1 cut(s) 408
Bsp13I TCCGGA 1 cut(s) 292
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 1 cut(s) 316
BspACI CCGC 3 cut(s) 186, 419, 439
BspDI ATCGAT 1 cut(s) 240
BspEI TCCGGA 1 cut(s) 292
BspFNI CGCG 1 cut(s) 217
BspLI GGNNCC 1 cut(s) 318
BspPI GGATC 2 cut(s) 311, 324
BspQI GCTCTTC 1 cut(s) 413
BsrGI TGTACA 1 cut(s) 3
BssECI CCNNGG 3 cut(s) 161, 414, 434
BssMI GATC 1 cut(s) 316
BssT1I CCWWGG 1 cut(s) 161
Bst2UI CCWGG 2 cut(s) 119, 388
Bst4CI ACNGT 2 cut(s) 45, 483
Bst6I CTCTTC 2 cut(s) 229, 413
BstAUI TGTACA 1 cut(s) 3
BstF5I GGATG 2 cut(s) 82, 109
BstFNI CGCG 1 cut(s) 217
BstH2I RGCGCY 1 cut(s) 286
BstHHI GCGC 1 cut(s) 285
BstKTI GATC 1 cut(s) 319
BstMBI GATC 1 cut(s) 316
BstMWI GCNNNNNNNGC 1 cut(s) 452
BstNI CCWGG 2 cut(s) 119, 388
BstSCI CCNGG 4 cut(s) 117, 386, 414, 434
BstUI CGCG 1 cut(s) 217
BstV2I GAAGAC 1 cut(s) 464
BstX2I RGATCY 1 cut(s) 316
BstYI RGATCY 1 cut(s) 316
Bsu15I ATCGAT 1 cut(s) 240
BsuTUI ATCGAT 1 cut(s) 240
BtsCI GGATG 2 cut(s) 82, 109
CaiI CAGNNNCTG 1 cut(s) 449
CfoI GCGC 1 cut(s) 285
ClaI ATCGAT 1 cut(s) 240
CseI GACGC 1 cut(s) 206
Csp6I GTAC 2 cut(s) 4, 332
CviAII CATG 1 cut(s) 56
CviJI RGCY 4 cut(s) 68, 302, 406, 446
CviKI_1 RGCY 4 cut(s) 68, 302, 406, 446
CviQI GTAC 2 cut(s) 4, 332
DpnI GATC 1 cut(s) 318
DpnII GATC 1 cut(s) 316
Eam1104I CTCTTC 2 cut(s) 229, 413
EarI CTCTTC 2 cut(s) 229, 413
EciI GGCGGA 2 cut(s) 201, 454
Eco130I CCWWGG 1 cut(s) 161
Eco24I GRGCYC 1 cut(s) 408
Eco47III AGCGCT 1 cut(s) 284
EcoRII CCWGG 2 cut(s) 117, 386
EcoT14I CCWWGG 1 cut(s) 161
EcoT38I GRGCYC 1 cut(s) 408
ErhI CCWWGG 1 cut(s) 161
FaeI CATG 1 cut(s) 59
FaiI YATR 8 cut(s) 57, 245, 247, 279, 299, 312, 338, 350
FatI CATG 1 cut(s) 55
FblI GTMKAC 1 cut(s) 430
FokI GGATG 2 cut(s) 89, 116
FriOI GRGCYC 1 cut(s) 408
GlaI GCGC 1 cut(s) 284
GsuI CTGGAG 1 cut(s) 471
HaeII RGCGCY 1 cut(s) 286
HapII CCGG 3 cut(s) 293, 416, 436
HgaI GACGC 1 cut(s) 206
HhaI GCGC 1 cut(s) 285
Hin1II CATG 1 cut(s) 59
Hin6I GCGC 1 cut(s) 283
HinP1I GCGC 1 cut(s) 283
HincII GTYRAC 1 cut(s) 468
HindII GTYRAC 1 cut(s) 468
HinfI GANTC 1 cut(s) 391
HpaI GTTAAC 1 cut(s) 468
HpaII CCGG 3 cut(s) 293, 416, 436
HphI GGTGA 2 cut(s) 125, 277
Hpy166II GTNNAC 3 cut(s) 334, 431, 468
Hpy188I TCNGA 2 cut(s) 52, 375
Hpy188III TCNNGA 2 cut(s) 293, 450
Hpy8I GTNNAC 3 cut(s) 334, 431, 468
HpyAV CCTTC 1 cut(s) 160
HpyCH4III ACNGT 2 cut(s) 45, 483
HpyCH4V TGCA 3 cut(s) 328, 361, 400
HpyF10VI GCNNNNNNNGC 1 cut(s) 452
Hsp92II CATG 1 cut(s) 59
HspAI GCGC 1 cut(s) 283
Kpn2I TCCGGA 1 cut(s) 292
KspAI GTTAAC 1 cut(s) 468
Kzo9I GATC 1 cut(s) 316
LguI GCTCTTC 1 cut(s) 413
LmnI GCTCC 1 cut(s) 452
LpnPI CCDG 9 cut(s) 104, 131, 306, 334, 373, 400, 429, 435, 449
MaeIII GTNAC 2 cut(s) 39, 218
MalI GATC 1 cut(s) 318
MboI GATC 1 cut(s) 316
MboII GAAGA 5 cut(s) 77, 216, 245, 400, 469
MfeI CAATTG 1 cut(s) 7
MflI RGATCY 1 cut(s) 316
MhlI GDGCHC 1 cut(s) 408
MluCI AATT 4 cut(s) 7, 33, 107, 252
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 5 cut(s) 93, 146, 176, 188, 198
MroI TCCGGA 1 cut(s) 292
MseI TTAA 2 cut(s) 32, 467
MslI CAYNNNNRTG 3 cut(s) 263, 302, 339
MspI CCGG 3 cut(s) 293, 416, 436
MspR9I CCNGG 4 cut(s) 119, 388, 416, 436
MunI CAATTG 1 cut(s) 7
MvaI CCWGG 2 cut(s) 119, 388
MvnI CGCG 1 cut(s) 217
MwoI GCNNNNNNNGC 1 cut(s) 452
NciI CCSGG 2 cut(s) 416, 436
NdeII GATC 1 cut(s) 316
NlaIII CATG 1 cut(s) 59
NlaIV GGNNCC 1 cut(s) 318
NmuCI GTSAC 1 cut(s) 218
OliI CACNNNNGTG 1 cut(s) 263
PciSI GCTCTTC 1 cut(s) 413
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PfeI GAWTC 1 cut(s) 391
PfoI TCCNGGA 1 cut(s) 386
PshBI ATTAAT 1 cut(s) 32
PsiI TTATAA 1 cut(s) 279
Psp6I CCWGG 2 cut(s) 117, 386
PspGI CCWGG 2 cut(s) 117, 386
PspN4I GGNNCC 1 cut(s) 318
PstNI CAGNNNCTG 1 cut(s) 449
PsuI RGATCY 1 cut(s) 316
RsaI GTAC 2 cut(s) 5, 333
RsaNI GTAC 2 cut(s) 4, 332
RseI CAYNNNNRTG 3 cut(s) 263, 302, 339
SapI GCTCTTC 1 cut(s) 413
SaqAI TTAA 2 cut(s) 32, 467
Sau3AI GATC 1 cut(s) 316
ScrFI CCNGG 4 cut(s) 119, 388, 416, 436
SduI GDGCHC 1 cut(s) 408
SetI ASST 5 cut(s) 66, 138, 171, 304, 448
SmiMI CAYNNNNRTG 3 cut(s) 263, 302, 339
Sse9I AATT 4 cut(s) 7, 33, 107, 252
SsiI CCGC 3 cut(s) 186, 419, 439
StyD4I CCNGG 4 cut(s) 117, 386, 414, 434
StyI CCWWGG 1 cut(s) 161
TaaI ACNGT 2 cut(s) 45, 483
TaqI TCGA 1 cut(s) 240
TasI AATT 4 cut(s) 7, 33, 107, 252
TatI WGTACW 2 cut(s) 3, 331
TfiI GAWTC 1 cut(s) 391
Tru1I TTAA 2 cut(s) 32, 467
Tru9I TTAA 2 cut(s) 32, 467
TseFI GTSAC 1 cut(s) 218
Tsp45I GTSAC 1 cut(s) 218
TspDTI ATGAA 2 cut(s) 18, 120
VspI ATTAAT 1 cut(s) 32
XapI RAATTY 1 cut(s) 107
XmiI GTMKAC 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.