RLG00000034332

Lysosomal Pro-X

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0012672
Physical Location & Seq
Reverse (-)
40849310 .. 40851365
2056 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034332

Sequence Viewer

Length: 567 bp
ATGCACGTAGTGCTTGCTTCATGGTTCCGTCTAAAGTATCCTCATGTTGCTCTTGGAGCTTTGGCCTCCTCAACCCCAGTCCTCTTATTTCCTAACATTGTTATTCCACTAGAGAAGGAATACCTTTCCATTGTTAGCAAGGATTTTCGAGATACAAGTGAGAGTTGCTACCAGACTATCAAAAGGTCATGGTCTGAATTTGATGAGATTGCTTCTCACCCAAACGGACTCTCGTTTCTCAGCAAATGTTCCGTACCTGTAGGCCTGAATTTGGATGCAAATCCTTTCGGTGGGTATATAGTTCCTTCTGATTTCTCCTGGTTGCCCAAAACCCCAATTCCTTCTTCTCAAATCCCGGCGAAATCCCATCAATCCCCACAACTTATACTTGTAAAATGGTGCCGTCTAACTCCTCAGAGAAGAATCTTTCTTTATTCAGCCATTAGAGCTTCGAACGCAAGCGCTGTCGCCGAGTCTTCTTCTGCTTCTCCGGGACTCTACTCGGCCAAGCAATTTGAGCTCACAGTTCCCAACATTGACTTGGTTCTCGAGGACGTCCGCCCCTAG

Protein Analysis

189

Amino Acids

20.82

Weight (kDa)

8.44

Isoelectric Point (pI)

56.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S28 PF05577 4 - 80 4.2e-13 Serine carboxypeptidase S28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 558
AccB1I GGYRCC 1 cut(s) 399
AciI CCGC 1 cut(s) 559
AcoI YGGCCR 1 cut(s) 504
AcsI RAATTY 2 cut(s) 197, 268
AcyI GRCGYC 1 cut(s) 555
AdeI CACNNNGTG 1 cut(s) 10
AfaI GTAC 1 cut(s) 255
AfeI AGCGCT 1 cut(s) 463
AfiI CCNNNNNNNGG 2 cut(s) 271, 290
AjnI CCWGG 1 cut(s) 317
AjuI GAANNNNNNNTTGG 2 cut(s) 328, 360
AluBI AGCT 3 cut(s) 59, 449, 520
AluI AGCT 3 cut(s) 59, 449, 520
Alw21I GWGCWC 1 cut(s) 522
Ama87I CYCGRG 1 cut(s) 548
Aor51HI AGCGCT 1 cut(s) 463
AoxI GGCC 3 cut(s) 63, 262, 504
ApoI RAATTY 2 cut(s) 197, 268
AspLEI GCGC 1 cut(s) 464
AsuC2I CCSGG 2 cut(s) 356, 492
AsuHPI GGTGA 1 cut(s) 209
AsuII TTCGAA 1 cut(s) 452
AvaI CYCGRG 1 cut(s) 548
BanI GGYRCC 1 cut(s) 399
BanII GRGCYC 1 cut(s) 522
BbsI GAAGAC 1 cut(s) 468
Bbv12I GWGCWC 1 cut(s) 522
BccI CCATC 1 cut(s) 375
BceAI ACGGC 1 cut(s) 387
BciT130I CCWGG 1 cut(s) 319
BciVI GTATCC 1 cut(s) 48
BcnI CCSGG 2 cut(s) 356, 492
BfaI CTAG 2 cut(s) 110, 565
BfmI CTRYAG 1 cut(s) 258
BfoI RGCGCY 1 cut(s) 465
BfuI GTATCC 1 cut(s) 48
Bme1390I CCNGG 3 cut(s) 319, 356, 492
BmeT110I CYCGRG 1 cut(s) 548
BmiI GGNNCC 2 cut(s) 26, 401
BmrFI CCNGG 3 cut(s) 319, 356, 492
BmrI ACTGGG 1 cut(s) 71
BmsI GCATC 1 cut(s) 265
BmuI ACTGGG 1 cut(s) 71
BpiI GAAGAC 1 cut(s) 468
Bpu14I TTCGAA 1 cut(s) 452
BpuMI CCSGG 2 cut(s) 356, 492
BsaAI YACGTR 1 cut(s) 7
BsaBI GATNNNNATC 1 cut(s) 279
BsaHI GRCGYC 1 cut(s) 555
Bsc4I CCNNNNNNNGG 2 cut(s) 271, 290
Bse1I ACTGG 1 cut(s) 77
Bse8I GATNNNNATC 1 cut(s) 279
BseBI CCWGG 1 cut(s) 319
BseGI GGATG 1 cut(s) 280
BseJI GATNNNNATC 1 cut(s) 279
BseLI CCNNNNNNNGG 2 cut(s) 271, 290
BseMII CTCAG 2 cut(s) 253, 428
BseNI ACTGG 1 cut(s) 77
BseRI GAGGAG 2 cut(s) 58, 402
BshFI GGCC 3 cut(s) 65, 264, 506
BshNI GGYRCC 1 cut(s) 399
BsiHKAI GWGCWC 1 cut(s) 522
BsiHKCI CYCGRG 1 cut(s) 548
BsiSI CCGG 2 cut(s) 356, 491
BslFI GGGAC 1 cut(s) 507
BslI CCNNNNNNNGG 2 cut(s) 271, 290
BsmFI GGGAC 1 cut(s) 507
BsnI GGCC 3 cut(s) 65, 264, 506
BsoBI CYCGRG 1 cut(s) 548
Bsp119I TTCGAA 1 cut(s) 452
Bsp1286I GDGCHC 1 cut(s) 522
BspACI CCGC 1 cut(s) 559
BspANI GGCC 3 cut(s) 65, 264, 506
BspCNI CTCAG 2 cut(s) 252, 427
BspLI GGNNCC 2 cut(s) 26, 401
BspT104I TTCGAA 1 cut(s) 452
BspT107I GGYRCC 1 cut(s) 399
BsrI ACTGG 1 cut(s) 77
BssNI GRCGYC 1 cut(s) 555
Bst2UI CCWGG 1 cut(s) 319
Bst4CI ACNGT 1 cut(s) 526
BstACI GRCGYC 1 cut(s) 555
BstAPI GCANNNNNTGC 1 cut(s) 10
BstBAI YACGTR 1 cut(s) 7
BstBI TTCGAA 1 cut(s) 452
BstC8I GCNNGC 2 cut(s) 15, 460
BstDEI CTNAG 2 cut(s) 239, 414
BstF5I GGATG 1 cut(s) 280
BstH2I RGCGCY 1 cut(s) 465
BstHHI GCGC 1 cut(s) 464
BstMWI GCNNNNNNNGC 5 cut(s) 10, 56, 446, 455, 517
BstNI CCWGG 1 cut(s) 319
BstSCI CCNGG 3 cut(s) 317, 354, 490
BstSFI CTRYAG 1 cut(s) 258
BstV2I GAAGAC 1 cut(s) 468
BsuI GTATCC 1 cut(s) 48
BsuRI GGCC 3 cut(s) 65, 264, 506
BtsCI GGATG 1 cut(s) 280
Cac8I GCNNGC 2 cut(s) 15, 460
CfoI GCGC 1 cut(s) 464
Csp6I GTAC 1 cut(s) 254
CviAII CATG 3 cut(s) 21, 44, 189
CviJI RGCY 7 cut(s) 59, 65, 264, 440, 449, 506, 520
CviKI_1 RGCY 7 cut(s) 59, 65, 264, 440, 449, 506, 520
CviQI GTAC 1 cut(s) 254
DdeI CTNAG 2 cut(s) 239, 414
DraIII CACNNNGTG 1 cut(s) 10
EaeI YGGCCR 1 cut(s) 504
EciI GGCGGA 1 cut(s) 548
Ecl136II GAGCTC 1 cut(s) 520
Eco147I AGGCCT 1 cut(s) 264
Eco24I GRGCYC 1 cut(s) 522
Eco47III AGCGCT 1 cut(s) 463
Eco53kI GAGCTC 1 cut(s) 520
Eco88I CYCGRG 1 cut(s) 548
EcoICRI GAGCTC 1 cut(s) 520
EcoRII CCWGG 1 cut(s) 317
EcoT38I GRGCYC 1 cut(s) 522
FaeI CATG 3 cut(s) 24, 47, 192
FaiI YATR 6 cut(s) 22, 45, 190, 297, 299, 386
FaqI GGGAC 1 cut(s) 507
FatI CATG 3 cut(s) 20, 43, 188
FokI GGATG 1 cut(s) 287
FriOI GRGCYC 1 cut(s) 522
FspBI CTAG 2 cut(s) 110, 565
GlaI GCGC 1 cut(s) 463
HaeII RGCGCY 1 cut(s) 465
HaeIII GGCC 3 cut(s) 65, 264, 506
HapII CCGG 2 cut(s) 356, 491
HhaI GCGC 1 cut(s) 464
Hin1I GRCGYC 1 cut(s) 555
Hin1II CATG 3 cut(s) 24, 47, 192
Hin6I GCGC 1 cut(s) 462
HinP1I GCGC 1 cut(s) 462
HinfI GANTC 4 cut(s) 228, 423, 473, 495
HpaII CCGG 2 cut(s) 356, 491
HphI GGTGA 1 cut(s) 209
Hpy188I TCNGA 3 cut(s) 196, 310, 417
Hpy188III TCNNGA 2 cut(s) 149, 548
HpyAV CCTTC 3 cut(s) 109, 315, 351
HpyCH4III ACNGT 1 cut(s) 526
HpyCH4IV ACGT 2 cut(s) 6, 555
HpyCH4V TGCA 2 cut(s) 4, 278
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 56, 446, 455, 517
HpyF3I CTNAG 2 cut(s) 239, 414
HpySE526I ACGT 2 cut(s) 6, 555
Hsp92I GRCGYC 1 cut(s) 555
Hsp92II CATG 3 cut(s) 24, 47, 192
HspAI GCGC 1 cut(s) 462
LmnI GCTCC 1 cut(s) 56
LpnPI CCDG 8 cut(s) 90, 185, 270, 278, 304, 331, 369, 504
LweI GCATC 1 cut(s) 265
MaeI CTAG 2 cut(s) 110, 565
MaeII ACGT 2 cut(s) 6, 555
MboII GAAGA 4 cut(s) 336, 432, 468, 471
MhlI GDGCHC 1 cut(s) 522
MluCI AATT 4 cut(s) 197, 268, 336, 512
MlyI GAGTC 3 cut(s) 222, 482, 489
MnlI CCTC 6 cut(s) 51, 76, 79, 92, 423, 544
MspI CCGG 2 cut(s) 356, 491
MspR9I CCNGG 3 cut(s) 319, 356, 492
MvaI CCWGG 1 cut(s) 319
MwoI GCNNNNNNNGC 5 cut(s) 10, 56, 446, 455, 517
NciI CCSGG 2 cut(s) 356, 492
NlaIII CATG 3 cut(s) 24, 47, 192
NlaIV GGNNCC 2 cut(s) 26, 401
NmeAIII GCCGAG 2 cut(s) 482, 496
NspV TTCGAA 1 cut(s) 452
PaeR7I CTCGAG 1 cut(s) 548
PceI AGGCCT 1 cut(s) 264
PfeI GAWTC 1 cut(s) 423
PfoI TCCNGGA 1 cut(s) 490
PleI GAGTC 3 cut(s) 222, 481, 489
PpsI GAGTC 3 cut(s) 222, 481, 489
Ppu21I YACGTR 1 cut(s) 7
Psp124BI GAGCTC 1 cut(s) 522
Psp6I CCWGG 1 cut(s) 317
PspGI CCWGG 1 cut(s) 317
PspN4I GGNNCC 2 cut(s) 26, 401
RsaI GTAC 1 cut(s) 255
RsaNI GTAC 1 cut(s) 254
SacI GAGCTC 1 cut(s) 522
SchI GAGTC 3 cut(s) 222, 482, 489
ScrFI CCNGG 3 cut(s) 319, 356, 492
SduI GDGCHC 1 cut(s) 522
SetI ASST 8 cut(s) 9, 61, 126, 188, 259, 451, 522, 558
SfaNI GCATC 1 cut(s) 265
SfcI CTRYAG 1 cut(s) 258
Sfr274I CTCGAG 1 cut(s) 548
SfuI TTCGAA 1 cut(s) 452
SlaI CTCGAG 1 cut(s) 548
SmlI CTYRAG 1 cut(s) 548
SmoI CTYRAG 1 cut(s) 548
Sse9I AATT 4 cut(s) 197, 268, 336, 512
SseBI AGGCCT 1 cut(s) 264
SsiI CCGC 1 cut(s) 559
SspMI CTAG 2 cut(s) 110, 565
SstI GAGCTC 1 cut(s) 522
StuI AGGCCT 1 cut(s) 264
StyD4I CCNGG 3 cut(s) 317, 354, 490
TaaI ACNGT 1 cut(s) 526
TaiI ACGT 2 cut(s) 9, 558
TaqI TCGA 3 cut(s) 148, 452, 549
TasI AATT 4 cut(s) 197, 268, 336, 512
TfiI GAWTC 1 cut(s) 423
TspDTI ATGAA 1 cut(s) 9
TspGWI ACGGA 3 cut(s) 17, 240, 241
XapI RAATTY 2 cut(s) 197, 268
XcmI CCANNNNNNNNNTGG 1 cut(s) 538
XhoI CTCGAG 1 cut(s) 548
XspI CTAG 2 cut(s) 110, 565
ZraI GACGTC 1 cut(s) 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.