RLG00000004237

Belongs to the spermidine spermine synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0012672
Physical Location & Seq
Reverse (-)
57493518 .. 57503249
9732 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004237

Sequence Viewer

Length: 453 bp
ATGAGAGATGATGAAGATCCCAAGTCGTACGATGGGTCTGGTTTGGCCTTGGAGGTGTCAGTGTCGATCTCATGGATATTGCAGCAGAGAGCGAGAGGATCACAGCAGCACGGAAAGGTAGTCATTTTGGATGGCTATCTTCAACTCACAGAGAAGGATGAGTTTGCTTACCAGGAAATGCTTTCTCACCTTCCTCTCTGTTCCATTCCAAACCCAAAGAAGGTGTTGCTTGTAGGAGGCGCATATGGTGGGATTTTGCGAGAAGTATCGTGCCATCCTTTTGTTGAAACATGTGTCTATAACCGTTTTATCCCTAGCATAGCAGTTGGATATGAGGATCAAAGCGTGAATGTGCATATTGGTGACGCTCCATCTCGGAAACTTAAAACATCTTCCCGAATAACGTACATACAGGGCCTGGAGGCAAAGACCTTTCTGACCAACACTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00330 ko00410 ko00480 ko01100 map00270 map00330 map00410 map00480 map01100
Pfam Domains
Protein Families
Spermine_synt_N Spermine_synth

Protein Analysis

151

Amino Acids

16.63

Weight (kDa)

6.06

Isoelectric Point (pI)

40.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spermine_synth PF01564 54 - 123 5.9e-15 Spermine/spermidine synthase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 11, 106, 345
AfaI GTAC 2 cut(s) 29, 407
AfiI CCNNNNNNNGG 1 cut(s) 220
AflIII ACRYGT 1 cut(s) 290
AgsI TTSAA 2 cut(s) 143, 287
AjnI CCWGG 2 cut(s) 171, 417
AlwI GGATC 3 cut(s) 11, 106, 345
AlwNI CAGNNNCTG 1 cut(s) 418
AoxI GGCC 2 cut(s) 45, 415
ApeKI GCWGC 2 cut(s) 82, 106
AspLEI GCGC 1 cut(s) 242
AspS9I GGNCC 1 cut(s) 415
AsuHPI GGTGA 2 cut(s) 179, 374
BbvI GCAGC 2 cut(s) 94, 118
BccI CCATC 4 cut(s) 26, 125, 282, 379
BciT130I CCWGG 2 cut(s) 173, 419
BfaI CTAG 1 cut(s) 315
BisI GCNGC 2 cut(s) 83, 107
BlsI GCNGC 2 cut(s) 84, 108
Bme1390I CCNGG 2 cut(s) 173, 419
BmgT120I GGNCC 1 cut(s) 415
BmrFI CCNGG 2 cut(s) 173, 419
BpmI CTGGAG 1 cut(s) 440
BsaBI GATNNNNATC 2 cut(s) 15, 135
BsaJI CCNNGG 1 cut(s) 48
Bsc4I CCNNNNNNNGG 1 cut(s) 220
Bse8I GATNNNNATC 2 cut(s) 15, 135
BseBI CCWGG 2 cut(s) 173, 419
BseDI CCNNGG 1 cut(s) 48
BseGI GGATG 3 cut(s) 136, 163, 274
BseJI GATNNNNATC 2 cut(s) 15, 135
BseLI CCNNNNNNNGG 1 cut(s) 220
BseXI GCAGC 2 cut(s) 94, 118
BshFI GGCC 2 cut(s) 47, 417
BsiWI CGTACG 1 cut(s) 27
BslI CCNNNNNNNGG 1 cut(s) 220
BsnI GGCC 2 cut(s) 47, 417
Bsp143I GATC 4 cut(s) 16, 66, 98, 337
BspANI GGCC 2 cut(s) 47, 417
BspPI GGATC 3 cut(s) 11, 106, 345
BssECI CCNNGG 1 cut(s) 48
BssMI GATC 4 cut(s) 16, 66, 98, 337
BssT1I CCWWGG 1 cut(s) 48
Bst2UI CCWGG 2 cut(s) 173, 419
Bst4CI ACNGT 1 cut(s) 305
BstF5I GGATG 3 cut(s) 136, 163, 274
BstHHI GCGC 1 cut(s) 242
BstKTI GATC 4 cut(s) 19, 69, 101, 340
BstMBI GATC 4 cut(s) 16, 66, 98, 337
BstNI CCWGG 2 cut(s) 173, 419
BstNSI RCATGY 1 cut(s) 294
BstSCI CCNGG 2 cut(s) 171, 417
BstV1I GCAGC 2 cut(s) 94, 118
BstX2I RGATCY 1 cut(s) 16
BstYI RGATCY 1 cut(s) 16
BsuRI GGCC 2 cut(s) 47, 417
BtsCI GGATG 3 cut(s) 136, 163, 274
BtsIMutI CAGTG 1 cut(s) 66
CaiI CAGNNNCTG 1 cut(s) 418
CfoI GCGC 1 cut(s) 242
Cfr13I GGNCC 1 cut(s) 415
CseI GACGC 1 cut(s) 374
Csp6I GTAC 2 cut(s) 28, 406
CviAII CATG 2 cut(s) 72, 291
CviJI RGCY 3 cut(s) 47, 135, 417
CviKI_1 RGCY 3 cut(s) 47, 135, 417
CviQI GTAC 2 cut(s) 28, 406
DpnI GATC 4 cut(s) 18, 68, 100, 339
DpnII GATC 4 cut(s) 16, 66, 98, 337
Eco130I CCWWGG 1 cut(s) 48
EcoO109I RGGNCCY 1 cut(s) 415
EcoRII CCWGG 2 cut(s) 171, 417
EcoT14I CCWWGG 1 cut(s) 48
ErhI CCWWGG 1 cut(s) 48
FaeI CATG 2 cut(s) 75, 294
FaiI YATR 9 cut(s) 73, 244, 246, 292, 300, 320, 333, 357, 410
FatI CATG 2 cut(s) 71, 290
FauNDI CATATG 1 cut(s) 244
Fnu4HI GCNGC 2 cut(s) 83, 107
FokI GGATG 3 cut(s) 143, 170, 261
Fsp4HI GCNGC 2 cut(s) 83, 107
FspBI CTAG 1 cut(s) 315
GlaI GCGC 1 cut(s) 241
GluI GCNGC 2 cut(s) 83, 107
GsuI CTGGAG 1 cut(s) 440
HaeIII GGCC 2 cut(s) 47, 417
HgaI GACGC 1 cut(s) 374
HhaI GCGC 1 cut(s) 242
Hin1II CATG 2 cut(s) 75, 294
Hin6I GCGC 1 cut(s) 240
HinP1I GCGC 1 cut(s) 240
HphI GGTGA 2 cut(s) 179, 374
Hpy188I TCNGA 2 cut(s) 378, 438
Hpy188III TCNNGA 2 cut(s) 396, 450
HpyAV CCTTC 3 cut(s) 148, 200, 214
HpyCH4III ACNGT 1 cut(s) 305
HpyCH4IV ACGT 1 cut(s) 404
HpyCH4V TGCA 2 cut(s) 82, 355
HpySE526I ACGT 1 cut(s) 404
Hsp92II CATG 2 cut(s) 75, 294
HspAI GCGC 1 cut(s) 240
Kzo9I GATC 4 cut(s) 16, 66, 98, 337
LmnI GCTCC 1 cut(s) 373
LpnPI CCDG 6 cut(s) 24, 158, 185, 398, 404, 431
Lsp1109I GCAGC 2 cut(s) 94, 118
MaeI CTAG 1 cut(s) 315
MaeII ACGT 1 cut(s) 404
MaeIII GTNAC 1 cut(s) 362
MalI GATC 4 cut(s) 18, 68, 100, 339
MboI GATC 4 cut(s) 16, 66, 98, 337
MboII GAAGA 3 cut(s) 26, 131, 384
MflI RGATCY 1 cut(s) 16
MmeI TCCRAC 1 cut(s) 307
MnlI CCTC 6 cut(s) 46, 89, 204, 230, 328, 415
MseI TTAA 1 cut(s) 384
MslI CAYNNNNRTG 1 cut(s) 360
MspR9I CCNGG 2 cut(s) 173, 419
MvaI CCWGG 2 cut(s) 173, 419
NdeI CATATG 1 cut(s) 244
NdeII GATC 4 cut(s) 16, 66, 98, 337
NlaIII CATG 2 cut(s) 75, 294
NmuCI GTSAC 1 cut(s) 362
NspI RCATGY 1 cut(s) 294
PciI ACATGT 1 cut(s) 290
Pfl23II CGTACG 1 cut(s) 27
PkrI GCNGC 2 cut(s) 84, 108
PscI ACATGT 1 cut(s) 290
Psp6I CCWGG 2 cut(s) 171, 417
PspGI CCWGG 2 cut(s) 171, 417
PspLI CGTACG 1 cut(s) 27
PspPI GGNCC 1 cut(s) 415
PstNI CAGNNNCTG 1 cut(s) 418
PsuI RGATCY 1 cut(s) 16
RsaI GTAC 2 cut(s) 29, 407
RsaNI GTAC 2 cut(s) 28, 406
RseI CAYNNNNRTG 1 cut(s) 360
SaqAI TTAA 1 cut(s) 384
SatI GCNGC 2 cut(s) 83, 107
Sau3AI GATC 4 cut(s) 16, 66, 98, 337
Sau96I GGNCC 1 cut(s) 415
ScrFI CCNGG 2 cut(s) 173, 419
SetI ASST 6 cut(s) 57, 120, 192, 225, 407, 434
SmiMI CAYNNNNRTG 1 cut(s) 360
SspMI CTAG 1 cut(s) 315
StyD4I CCNGG 2 cut(s) 171, 417
StyI CCWWGG 1 cut(s) 48
TaaI ACNGT 1 cut(s) 305
TaiI ACGT 1 cut(s) 407
TaqI TCGA 1 cut(s) 65
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TscAI CASTG 1 cut(s) 66
TseFI GTSAC 1 cut(s) 362
TseI GCWGC 2 cut(s) 82, 106
Tsp45I GTSAC 1 cut(s) 362
TspDTI ATGAA 1 cut(s) 27
TspGWI ACGGA 1 cut(s) 126
TspRI CASTG 1 cut(s) 66
XceI RCATGY 1 cut(s) 294
XspI CTAG 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.