Rh2AG306900

Belongs to the spermidine spermine synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0012672
Physical Location & Seq
Reverse (-)
38674645 .. 38677555
2911 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG306900.1

Sequence Viewer

Length: 486 bp
ATGAAAATGGGGATTGAACGAGTGCTTAAAGAAAAGTTTGCAGATGCACTCAAGGATATTCAACAAGTCTTTGATGAAGAAAACAGAGAGACCATAGTTGAGGTTTTGGTTATCGGTGGAGGAGATGGTGGTCTAATGAGGGAATTGGCTCGTCATTCTTCTGTCGAGCAAATAGATATATGTGAGATTGATAAGTTGGTTGTTGACGTTTCAACACAGTTTTTCCCTCGTGTAGCTATTGGATATGAGGATCCCAGGGTGACACTCCATGTTGGTGATGGAGTCGCATTTTCAAAGGCTGTTCCAGCAGGAACTTATGATGCAATAATTATGGACTCTTCTGATCCCATAGGTAATACTGATGACTTTGCTTTTAGGACACCCAAGTTCTGGCAAATCCACTTTGCTCACGACCCTTGCCGGAAAACTGGACTCCAACTTAAAGAAATTAGGTGTTCTAAAAGTGTAAAGTTGAAGGAGGCTTAG

Protein Analysis

161

Amino Acids

18.0

Weight (kDa)

5.24

Isoelectric Point (pI)

22.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spermine_synth PF01564 33 - 128 8.9e-34 Spermine/spermidine synthase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 390
AclWI GGATC 3 cut(s) 245, 258, 338
AfiI CCNNNNNNNGG 1 cut(s) 390
AgsI TTSAA 5 cut(s) 17, 62, 213, 294, 475
AjnI CCWGG 1 cut(s) 254
AluBI AGCT 1 cut(s) 236
AluI AGCT 1 cut(s) 236
Alw26I GTCTC 1 cut(s) 83
AlwI GGATC 3 cut(s) 245, 258, 338
AsuHPI GGTGA 2 cut(s) 271, 287
BamHI GGATCC 1 cut(s) 250
BauI CACGAG 1 cut(s) 228
BccI CCATC 2 cut(s) 119, 272
BciT130I CCWGG 1 cut(s) 256
BcoDI GTCTC 1 cut(s) 83
Bme1390I CCNGG 1 cut(s) 256
BmiI GGNNCC 1 cut(s) 252
BmrFI CCNGG 1 cut(s) 256
BmsI GCATC 2 cut(s) 34, 310
BpuEI CTTGAG 1 cut(s) 35
BsaI GGTCTC 1 cut(s) 83
BsaJI CCNNGG 2 cut(s) 254, 255
Bsc4I CCNNNNNNNGG 1 cut(s) 390
Bse1I ACTGG 1 cut(s) 433
BseBI CCWGG 1 cut(s) 256
BseDI CCNNGG 2 cut(s) 254, 255
BseLI CCNNNNNNNGG 1 cut(s) 390
BseNI ACTGG 1 cut(s) 433
BseRI GAGGAG 1 cut(s) 135
BsiSI CCGG 1 cut(s) 421
BslI CCNNNNNNNGG 1 cut(s) 390
BsmAI GTCTC 1 cut(s) 83
Bso31I GGTCTC 1 cut(s) 83
Bsp143I GATC 2 cut(s) 250, 343
BspLI GGNNCC 1 cut(s) 252
BspPI GGATC 3 cut(s) 245, 258, 338
BspTNI GGTCTC 1 cut(s) 83
BsrI ACTGG 1 cut(s) 433
BssECI CCNNGG 2 cut(s) 254, 255
BssMI GATC 2 cut(s) 250, 343
BssSI CACGAG 1 cut(s) 228
Bst2BI CACGAG 1 cut(s) 228
Bst2UI CCWGG 1 cut(s) 256
Bst4CI ACNGT 1 cut(s) 219
Bst6I CTCTTC 1 cut(s) 343
BstDEI CTNAG 1 cut(s) 483
BstKTI GATC 2 cut(s) 253, 346
BstMAI GTCTC 1 cut(s) 83
BstMBI GATC 2 cut(s) 250, 343
BstMWI GCNNNNNNNGC 1 cut(s) 305
BstNI CCWGG 1 cut(s) 256
BstSCI CCNGG 1 cut(s) 254
BstX2I RGATCY 1 cut(s) 250
BstYI RGATCY 1 cut(s) 250
CviAII CATG 1 cut(s) 269
CviJI RGCY 4 cut(s) 149, 236, 299, 482
CviKI_1 RGCY 4 cut(s) 149, 236, 299, 482
DdeI CTNAG 1 cut(s) 483
DpnI GATC 2 cut(s) 252, 345
DpnII GATC 2 cut(s) 250, 343
Eam1104I CTCTTC 1 cut(s) 343
EarI CTCTTC 1 cut(s) 343
Eco31I GGTCTC 1 cut(s) 83
EcoRII CCWGG 1 cut(s) 254
FaeI CATG 1 cut(s) 272
FaiI YATR 8 cut(s) 95, 179, 181, 246, 270, 318, 332, 350
FatI CATG 1 cut(s) 268
HapII CCGG 1 cut(s) 421
Hin1II CATG 1 cut(s) 272
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HinfI GANTC 3 cut(s) 282, 335, 432
HpaII CCGG 1 cut(s) 421
HphI GGTGA 2 cut(s) 271, 287
Hpy166II GTNNAC 1 cut(s) 205
Hpy188I TCNGA 1 cut(s) 343
Hpy188III TCNNGA 1 cut(s) 410
Hpy8I GTNNAC 1 cut(s) 205
HpyAV CCTTC 1 cut(s) 469
HpyCH4III ACNGT 1 cut(s) 219
HpyCH4IV ACGT 1 cut(s) 207
HpyCH4V TGCA 3 cut(s) 41, 47, 323
HpyF10VI GCNNNNNNNGC 1 cut(s) 305
HpyF3I CTNAG 1 cut(s) 483
HpySE526I ACGT 1 cut(s) 207
Hsp92II CATG 1 cut(s) 272
Kzo9I GATC 2 cut(s) 250, 343
LpnPI CCDG 7 cut(s) 241, 268, 294, 318, 376, 414, 434
LweI GCATC 2 cut(s) 34, 310
MaeII ACGT 1 cut(s) 207
MaeIII GTNAC 1 cut(s) 259
MalI GATC 2 cut(s) 252, 345
MboI GATC 2 cut(s) 250, 343
MboII GAAGA 3 cut(s) 89, 150, 330
MflI RGATCY 1 cut(s) 250
MluCI AATT 3 cut(s) 143, 327, 447
MlyI GAGTC 3 cut(s) 291, 329, 426
MmeI TCCRAC 1 cut(s) 460
MnlI CCTC 6 cut(s) 94, 113, 132, 237, 241, 472
MseI TTAA 2 cut(s) 27, 441
MslI CAYNNNNRTG 1 cut(s) 273
MspI CCGG 1 cut(s) 421
MspR9I CCNGG 1 cut(s) 256
MvaI CCWGG 1 cut(s) 256
MwoI GCNNNNNNNGC 1 cut(s) 305
NdeII GATC 2 cut(s) 250, 343
NlaIII CATG 1 cut(s) 272
NlaIV GGNNCC 1 cut(s) 252
NmuCI GTSAC 1 cut(s) 259
PasI CCCWGGG 1 cut(s) 255
PflMI CCANNNNNTGG 1 cut(s) 390
PleI GAGTC 3 cut(s) 290, 329, 426
PpsI GAGTC 3 cut(s) 290, 329, 426
Psp6I CCWGG 1 cut(s) 254
PspGI CCWGG 1 cut(s) 254
PspN4I GGNNCC 1 cut(s) 252
PsuI RGATCY 1 cut(s) 250
RseI CAYNNNNRTG 1 cut(s) 273
SaqAI TTAA 2 cut(s) 27, 441
Sau3AI GATC 2 cut(s) 250, 343
SchI GAGTC 3 cut(s) 291, 329, 426
ScrFI CCNGG 1 cut(s) 256
SetI ASST 5 cut(s) 105, 210, 238, 355, 455
SfaNI GCATC 2 cut(s) 34, 310
SmiMI CAYNNNNRTG 1 cut(s) 273
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
Sse9I AATT 3 cut(s) 143, 327, 447
StyD4I CCNGG 1 cut(s) 254
TaaI ACNGT 1 cut(s) 219
TaiI ACGT 1 cut(s) 210
TaqI TCGA 1 cut(s) 165
TasI AATT 3 cut(s) 143, 327, 447
Tru1I TTAA 2 cut(s) 27, 441
Tru9I TTAA 2 cut(s) 27, 441
TseFI GTSAC 1 cut(s) 259
Tsp45I GTSAC 1 cut(s) 259
TspDTI ATGAA 2 cut(s) 17, 90
Van91I CCANNNNNTGG 1 cut(s) 390
XcmI CCANNNNNNNNNTGG 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.