RLG00000019516

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0012672
Physical Location & Seq
Forward (+)
53028896 .. 53030193
1298 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019516

Sequence Viewer

Length: 531 bp
ATGTATTTAGTTCCTTCTGATTTCTCCTGGTTGCCCAAAACCCCAATTCCTTCTTCTCAAATCCCAGCGAAATCCCATCAATCCCCACAACTTATGCTTGTAAAATGCTGCCGTCTAACTCCTCAAAGAAGAATCTTTCTCTATTCTGCCATTAGAGCTTCGAACGCAAGCGCTGCCGCCGAGTATTCTTCTGCTTCTCCGAGACTCTACTTGGCCAAGCAATTTGAGCTCACAGTTCCCAACATTGACTTGGTTCTCGAGGACTGTCGATGGATTTTCGTGAGGAGTGAGTTTATGATTGTAAGTGCAGGCAGTCTCGACATATTGAGGCCGGCGAAGAATTTTGAACTATTGATGCTATATGGTAGCATGTTGCCAATTGCAATACGTGCAGTTTTTCTAACTCAAATAGGAGAGGCACATTCCTTGAAGATAGAGAAAATTTTGTTTCAAGGCAAATCAGACTATCAGAATGTGATGGTCTTCCACTTATCAACCTATGGAAAGGTTCTCATCCTAGATAAGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

177

Amino Acids

19.93

Weight (kDa)

9.17

Isoelectric Point (pI)

47.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spermine_synt_N PF17284 138 - 175 3.2e-14 Spermidine synthase tetramerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 177
AcoI YGGCCR 1 cut(s) 213
AcsI RAATTY 2 cut(s) 340, 441
AfeI AGCGCT 1 cut(s) 172
AfiI CCNNNNNNNGG 1 cut(s) 523
AgsI TTSAA 3 cut(s) 347, 430, 452
AjnI CCWGG 1 cut(s) 26
AjuI GAANNNNNNNTTGG 2 cut(s) 37, 69
AluBI AGCT 2 cut(s) 158, 229
AluI AGCT 2 cut(s) 158, 229
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 2 cut(s) 196, 320
Ama87I CYCGRG 1 cut(s) 257
Aor51HI AGCGCT 1 cut(s) 172
AoxI GGCC 2 cut(s) 213, 329
ApeKI GCWGC 2 cut(s) 108, 173
ApoI RAATTY 2 cut(s) 340, 441
AspLEI GCGC 1 cut(s) 173
AsuII TTCGAA 1 cut(s) 161
AvaI CYCGRG 1 cut(s) 257
BalI TGGCCA 1 cut(s) 215
BanII GRGCYC 1 cut(s) 231
BbsI GAAGAC 1 cut(s) 475
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 2 cut(s) 95, 160
BccI CCATC 3 cut(s) 84, 264, 472
BceAI ACGGC 1 cut(s) 96
BciT130I CCWGG 1 cut(s) 28
BcoDI GTCTC 2 cut(s) 196, 320
BfaI CTAG 1 cut(s) 518
BfoI RGCGCY 1 cut(s) 174
BisI GCNGC 3 cut(s) 109, 174, 177
BlsI GCNGC 3 cut(s) 110, 175, 178
Bme1390I CCNGG 1 cut(s) 28
BmeT110I CYCGRG 1 cut(s) 257
BmrFI CCNGG 1 cut(s) 28
BmsI GCATC 1 cut(s) 345
BpiI GAAGAC 1 cut(s) 475
Bpu14I TTCGAA 1 cut(s) 161
BsaAI YACGTR 1 cut(s) 389
Bsc4I CCNNNNNNNGG 1 cut(s) 523
Bse118I RCCGGY 1 cut(s) 331
BseBI CCWGG 1 cut(s) 28
BseGI GGATG 1 cut(s) 513
BseLI CCNNNNNNNGG 1 cut(s) 523
BseRI GAGGAG 2 cut(s) 111, 298
BseXI GCAGC 2 cut(s) 95, 160
BseYI CCCAGC 1 cut(s) 64
BsgI GTGCAG 2 cut(s) 327, 411
BshFI GGCC 2 cut(s) 215, 331
BsiHKAI GWGCWC 1 cut(s) 231
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 1 cut(s) 332
BslI CCNNNNNNNGG 1 cut(s) 523
BsmAI GTCTC 2 cut(s) 196, 320
BsnI GGCC 2 cut(s) 215, 331
BsoBI CYCGRG 1 cut(s) 257
Bsp119I TTCGAA 1 cut(s) 161
Bsp1286I GDGCHC 1 cut(s) 231
BspACI CCGC 1 cut(s) 177
BspANI GGCC 2 cut(s) 215, 331
BspT104I TTCGAA 1 cut(s) 161
BsrFI RCCGGY 1 cut(s) 331
BssAI RCCGGY 1 cut(s) 331
Bst2UI CCWGG 1 cut(s) 28
Bst4CI ACNGT 2 cut(s) 235, 266
BstAPI GCANNNNNTGC 2 cut(s) 173, 389
BstBAI YACGTR 1 cut(s) 389
BstBI TTCGAA 1 cut(s) 161
BstC8I GCNNGC 3 cut(s) 169, 310, 333
BstENI CCTNNNNNAGG 1 cut(s) 521
BstF5I GGATG 1 cut(s) 513
BstH2I RGCGCY 1 cut(s) 174
BstHHI GCGC 1 cut(s) 173
BstMAI GTCTC 2 cut(s) 196, 320
BstMWI GCNNNNNNNGC 5 cut(s) 155, 164, 173, 226, 389
BstNI CCWGG 1 cut(s) 28
BstNSI RCATGY 1 cut(s) 373
BstSCI CCNGG 1 cut(s) 26
BstV1I GCAGC 2 cut(s) 95, 160
BstV2I GAAGAC 1 cut(s) 475
BsuRI GGCC 2 cut(s) 215, 331
BtsCI GGATG 1 cut(s) 513
Cac8I GCNNGC 3 cut(s) 169, 310, 333
CfoI GCGC 1 cut(s) 173
Cfr10I RCCGGY 1 cut(s) 331
CviAII CATG 1 cut(s) 370
CviJI RGCY 4 cut(s) 158, 215, 229, 331
CviKI_1 RGCY 4 cut(s) 158, 215, 229, 331
EaeI YGGCCR 1 cut(s) 213
Ecl136II GAGCTC 1 cut(s) 229
Eco24I GRGCYC 1 cut(s) 231
Eco47III AGCGCT 1 cut(s) 172
Eco53kI GAGCTC 1 cut(s) 229
Eco88I CYCGRG 1 cut(s) 257
EcoICRI GAGCTC 1 cut(s) 229
EcoNI CCTNNNNNAGG 1 cut(s) 521
EcoRII CCWGG 1 cut(s) 26
EcoT38I GRGCYC 1 cut(s) 231
FaeI CATG 1 cut(s) 373
FaiI YATR 7 cut(s) 95, 296, 323, 361, 363, 371, 501
FatI CATG 1 cut(s) 369
Fnu4HI GCNGC 3 cut(s) 109, 174, 177
FokI GGATG 1 cut(s) 500
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 3 cut(s) 109, 174, 177
FspBI CTAG 1 cut(s) 518
GlaI GCGC 1 cut(s) 172
GluI GCNGC 3 cut(s) 109, 174, 177
GsaI CCCAGC 1 cut(s) 68
HaeII RGCGCY 1 cut(s) 174
HaeIII GGCC 2 cut(s) 215, 331
HapII CCGG 1 cut(s) 332
HhaI GCGC 1 cut(s) 173
Hin1II CATG 1 cut(s) 373
Hin6I GCGC 1 cut(s) 171
HinP1I GCGC 1 cut(s) 171
HinfI GANTC 2 cut(s) 132, 204
HpaII CCGG 1 cut(s) 332
Hpy188I TCNGA 4 cut(s) 19, 201, 463, 471
Hpy188III TCNNGA 3 cut(s) 257, 280, 317
HpyAV CCTTC 2 cut(s) 24, 60
HpyCH4III ACNGT 2 cut(s) 235, 266
HpyCH4IV ACGT 1 cut(s) 388
HpyCH4V TGCA 3 cut(s) 308, 383, 392
HpyF10VI GCNNNNNNNGC 5 cut(s) 155, 164, 173, 226, 389
HpySE526I ACGT 1 cut(s) 388
Hsp92II CATG 1 cut(s) 373
HspAI GCGC 1 cut(s) 171
KroI GCCGGC 1 cut(s) 331
KroNI GCCGGC 1 cut(s) 333
LpnPI CCDG 5 cut(s) 13, 40, 78, 294, 345
Lsp1109I GCAGC 2 cut(s) 95, 160
LweI GCATC 1 cut(s) 345
MaeI CTAG 1 cut(s) 518
MaeII ACGT 1 cut(s) 388
MboII GAAGA 6 cut(s) 45, 141, 180, 349, 442, 475
MfeI CAATTG 1 cut(s) 378
MhlI GDGCHC 1 cut(s) 231
MlsI TGGCCA 1 cut(s) 215
MluCI AATT 5 cut(s) 45, 221, 340, 378, 441
MluNI TGGCCA 1 cut(s) 215
MlyI GAGTC 1 cut(s) 198
MnlI CCTC 5 cut(s) 132, 253, 276, 321, 409
Mox20I TGGCCA 1 cut(s) 215
MroNI GCCGGC 1 cut(s) 331
MscI TGGCCA 1 cut(s) 215
Msp20I TGGCCA 1 cut(s) 215
MspI CCGG 1 cut(s) 332
MspR9I CCNGG 1 cut(s) 28
MunI CAATTG 1 cut(s) 378
MvaI CCWGG 1 cut(s) 28
MwoI GCNNNNNNNGC 5 cut(s) 155, 164, 173, 226, 389
NaeI GCCGGC 1 cut(s) 333
NgoMIV GCCGGC 1 cut(s) 331
NlaIII CATG 1 cut(s) 373
NmeAIII GCCGAG 1 cut(s) 205
NspI RCATGY 1 cut(s) 373
NspV TTCGAA 1 cut(s) 161
PaeR7I CTCGAG 1 cut(s) 257
PdiI GCCGGC 1 cut(s) 333
PfeI GAWTC 1 cut(s) 132
PkrI GCNGC 3 cut(s) 110, 175, 178
PleI GAGTC 1 cut(s) 198
PpsI GAGTC 1 cut(s) 198
Ppu21I YACGTR 1 cut(s) 389
Psp124BI GAGCTC 1 cut(s) 231
Psp6I CCWGG 1 cut(s) 26
PspFI CCCAGC 1 cut(s) 64
PspGI CCWGG 1 cut(s) 26
SacI GAGCTC 1 cut(s) 231
SatI GCNGC 3 cut(s) 109, 174, 177
SchI GAGTC 1 cut(s) 198
ScrFI CCNGG 1 cut(s) 28
SduI GDGCHC 1 cut(s) 231
SetI ASST 5 cut(s) 160, 231, 391, 500, 510
SfaNI GCATC 1 cut(s) 345
Sfr274I CTCGAG 1 cut(s) 257
SfuI TTCGAA 1 cut(s) 161
SlaI CTCGAG 1 cut(s) 257
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 5 cut(s) 45, 221, 340, 378, 441
SsiI CCGC 1 cut(s) 177
SspMI CTAG 1 cut(s) 518
SstI GAGCTC 1 cut(s) 231
StyD4I CCNGG 1 cut(s) 26
TaaI ACNGT 2 cut(s) 235, 266
TaiI ACGT 1 cut(s) 391
TaqI TCGA 4 cut(s) 161, 258, 268, 318
TasI AATT 5 cut(s) 45, 221, 340, 378, 441
TauI GCSGC 1 cut(s) 179
TfiI GAWTC 1 cut(s) 132
TseI GCWGC 2 cut(s) 108, 173
XagI CCTNNNNNAGG 1 cut(s) 521
XapI RAATTY 2 cut(s) 340, 441
XceI RCATGY 1 cut(s) 373
XcmI CCANNNNNNNNNTGG 1 cut(s) 247
XhoI CTCGAG 1 cut(s) 257
XspI CTAG 1 cut(s) 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.