Rh7BG361300

Belongs to the spermidine spermine synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0012672
Physical Location & Seq
Reverse (-)
38249537 .. 38253184
3648 bp
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UTR
Exon/CDS
Intron
Rh7BG361300.1

Sequence Viewer

Length: 462 bp
ATGAAAATGGGGATTGAACGAGTGCTTAAAGAAAAGTCTGGAGATGCACTCAAGGATATTCAACAAGTCTTTGATGAAGAAAACAGAATGACCACAGTTGAGGTTCTCGTCCTGGATGGGGTGATTCAGCTGACAAAAAGGGATGAATGTGTCTATCAAGAAATCATTACTCATCTTCCTCTCTGCCCAATTCCAAACCCCAAGAAGGTTTTGGTTATCGGTGGAGGAGATGGTGGTGTACTGAGGGAAGTGGCTAGTCATTCTTCTATCAAGCAAATAGATATATGTGAGATTGATAAGATGGTTGTTGATGTTTCAAAACAGTTTTTCTCTCGTGTAGCTATTGGATATGAGGATCCCAGGGTGACACTCCATGTTGGTGATGGAGTCGCATTTTCAAAGGCTGTTCTAGCAGGAACTTATGATGCAATAATTGTGGACTCTTCTGATCCCATAGTTTGA

Protein Analysis

153

Amino Acids

16.77

Weight (kDa)

4.92

Isoelectric Point (pI)

35.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spermine_synth PF01564 49 - 152 2e-42 Spermine/spermidine synthase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 350, 363, 443
AfaI GTAC 1 cut(s) 240
AfiI CCNNNNNNNGG 2 cut(s) 118, 205
AgsI TTSAA 4 cut(s) 17, 62, 318, 399
AjnI CCWGG 2 cut(s) 111, 359
AluBI AGCT 2 cut(s) 130, 341
AluI AGCT 2 cut(s) 130, 341
AlwI GGATC 3 cut(s) 350, 363, 443
AsuHPI GGTGA 3 cut(s) 133, 376, 392
BamHI GGATCC 1 cut(s) 355
BauI CACGAG 1 cut(s) 333
BccI CCATC 4 cut(s) 110, 224, 295, 377
BciT130I CCWGG 2 cut(s) 113, 361
BfaI CTAG 2 cut(s) 255, 410
Bme1390I CCNGG 2 cut(s) 113, 361
BmiI GGNNCC 1 cut(s) 357
BmrFI CCNGG 2 cut(s) 113, 361
BmsI GCATC 2 cut(s) 34, 415
BplI GAGNNNNNCTC 2 cut(s) 33, 65
BpmI CTGGAG 1 cut(s) 60
BpuEI CTTGAG 1 cut(s) 35
BsaJI CCNNGG 2 cut(s) 359, 360
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 205
BseBI CCWGG 2 cut(s) 113, 361
BseDI CCNNGG 2 cut(s) 359, 360
BseGI GGATG 2 cut(s) 121, 148
BseLI CCNNNNNNNGG 2 cut(s) 118, 205
BseMII CTCAG 1 cut(s) 233
BseRI GAGGAG 1 cut(s) 240
BslI CCNNNNNNNGG 2 cut(s) 118, 205
Bsp143I GATC 2 cut(s) 355, 448
BspCNI CTCAG 1 cut(s) 234
BspLI GGNNCC 1 cut(s) 357
BspPI GGATC 3 cut(s) 350, 363, 443
BssECI CCNNGG 2 cut(s) 359, 360
BssMI GATC 2 cut(s) 355, 448
BssSI CACGAG 1 cut(s) 333
Bst2BI CACGAG 1 cut(s) 333
Bst2UI CCWGG 2 cut(s) 113, 361
Bst4CI ACNGT 2 cut(s) 97, 324
Bst6I CTCTTC 1 cut(s) 448
BstDEI CTNAG 1 cut(s) 242
BstF5I GGATG 2 cut(s) 121, 148
BstKTI GATC 2 cut(s) 358, 451
BstMBI GATC 2 cut(s) 355, 448
BstMWI GCNNNNNNNGC 1 cut(s) 410
BstNI CCWGG 2 cut(s) 113, 361
BstSCI CCNGG 2 cut(s) 111, 359
BstX2I RGATCY 1 cut(s) 355
BstYI RGATCY 1 cut(s) 355
BtsCI GGATG 2 cut(s) 121, 148
Csp6I GTAC 1 cut(s) 239
CspCI CAANNNNNGTGG 2 cut(s) 417, 452
CviAII CATG 1 cut(s) 374
CviJI RGCY 4 cut(s) 130, 254, 341, 404
CviKI_1 RGCY 4 cut(s) 130, 254, 341, 404
CviQI GTAC 1 cut(s) 239
DdeI CTNAG 1 cut(s) 242
DpnI GATC 2 cut(s) 357, 450
DpnII GATC 2 cut(s) 355, 448
Eam1104I CTCTTC 1 cut(s) 448
EarI CTCTTC 1 cut(s) 448
EcoRII CCWGG 2 cut(s) 111, 359
FaeI CATG 1 cut(s) 377
FaiI YATR 6 cut(s) 284, 286, 351, 375, 423, 455
FatI CATG 1 cut(s) 373
FokI GGATG 2 cut(s) 128, 155
FspBI CTAG 2 cut(s) 255, 410
GsuI CTGGAG 1 cut(s) 60
Hin1II CATG 1 cut(s) 377
HinfI GANTC 3 cut(s) 124, 387, 440
HphI GGTGA 3 cut(s) 133, 376, 392
Hpy166II GTNNAC 2 cut(s) 239, 439
Hpy188I TCNGA 1 cut(s) 448
Hpy188III TCNNGA 2 cut(s) 39, 158
Hpy8I GTNNAC 2 cut(s) 239, 439
HpyAV CCTTC 1 cut(s) 199
HpyCH4III ACNGT 2 cut(s) 97, 324
HpyCH4V TGCA 2 cut(s) 47, 428
HpyF10VI GCNNNNNNNGC 1 cut(s) 410
HpyF3I CTNAG 1 cut(s) 242
Hsp92II CATG 1 cut(s) 377
Kzo9I GATC 2 cut(s) 355, 448
LpnPI CCDG 6 cut(s) 24, 98, 125, 346, 373, 399
LweI GCATC 2 cut(s) 34, 415
MaeI CTAG 2 cut(s) 255, 410
MaeIII GTNAC 1 cut(s) 364
MalI GATC 2 cut(s) 357, 450
MboI GATC 2 cut(s) 355, 448
MboII GAAGA 4 cut(s) 89, 167, 255, 435
MflI RGATCY 1 cut(s) 355
MluCI AATT 2 cut(s) 189, 432
MlyI GAGTC 2 cut(s) 396, 434
MnlI CCTC 5 cut(s) 94, 189, 218, 237, 346
MseI TTAA 1 cut(s) 27
MslI CAYNNNNRTG 1 cut(s) 378
MspA1I CMGCKG 1 cut(s) 130
MspR9I CCNGG 2 cut(s) 113, 361
MvaI CCWGG 2 cut(s) 113, 361
MwoI GCNNNNNNNGC 1 cut(s) 410
NdeII GATC 2 cut(s) 355, 448
NlaIII CATG 1 cut(s) 377
NlaIV GGNNCC 1 cut(s) 357
NmuCI GTSAC 1 cut(s) 364
PasI CCCWGGG 1 cut(s) 360
PfeI GAWTC 1 cut(s) 124
PfoI TCCNGGA 1 cut(s) 111
PleI GAGTC 2 cut(s) 395, 434
PpsI GAGTC 2 cut(s) 395, 434
Psp6I CCWGG 2 cut(s) 111, 359
PspGI CCWGG 2 cut(s) 111, 359
PspN4I GGNNCC 1 cut(s) 357
PsuI RGATCY 1 cut(s) 355
PvuII CAGCTG 1 cut(s) 130
RsaI GTAC 1 cut(s) 240
RsaNI GTAC 1 cut(s) 239
RseI CAYNNNNRTG 1 cut(s) 378
SaqAI TTAA 1 cut(s) 27
Sau3AI GATC 2 cut(s) 355, 448
SchI GAGTC 2 cut(s) 396, 434
ScrFI CCNGG 2 cut(s) 113, 361
SetI ASST 4 cut(s) 105, 132, 210, 343
SfaNI GCATC 2 cut(s) 34, 415
SmiMI CAYNNNNRTG 1 cut(s) 378
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
Sse9I AATT 2 cut(s) 189, 432
SspMI CTAG 2 cut(s) 255, 410
StyD4I CCNGG 2 cut(s) 111, 359
TaaI ACNGT 2 cut(s) 97, 324
TasI AATT 2 cut(s) 189, 432
TatI WGTACW 1 cut(s) 238
TfiI GAWTC 1 cut(s) 124
Tru1I TTAA 1 cut(s) 27
Tru9I TTAA 1 cut(s) 27
TseFI GTSAC 1 cut(s) 364
Tsp45I GTSAC 1 cut(s) 364
TspDTI ATGAA 3 cut(s) 17, 90, 159
XcmI CCANNNNNNNNNTGG 2 cut(s) 208, 380
XspI CTAG 2 cut(s) 255, 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.