Rh5BG138000

Belongs to the spermidine spermine synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0012672
Physical Location & Seq
Forward (+)
14177588 .. 14183265
5678 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG138000.1

Sequence Viewer

Length: 399 bp
ATGGGGATTGAACGAGTGCTTAAAGAAAAGTTTGCAGATGCACTCAAGGATATTCAACAAGTCTTTGATGAAGAAAACAGAGAGACTACAGTTGAGTTATCAACCTATGGAAAGGTTCTCATCCTAGATAGGGTTTTGGTTATCGGTGAAGGAGATGGTGGTGTAATGAGGGAAGTGGCTCGTCATTCTTCTGTCGAGCAAATAGATATATGTGAGATTGATAAGTTGGTTGTTGATGTTTCAAAACAGTTTTTCCCTCGTGTAGCTATTAGATATGAGGATCCCAGGGTGACACTCCATGTTGGTGATGGAGTCGCATTTTCAAAGGCTGTTCCAGCAGGAACTTATGATGCAATAATTGTGGACTCTTCTGATCCCATAGGTACATTTGAATATTAG

Protein Analysis

132

Amino Acids

14.69

Weight (kDa)

4.59

Isoelectric Point (pI)

23.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spermine_synth PF01564 44 - 130 1.6e-32 Spermine/spermidine synthase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 275, 288, 368
AfaI GTAC 1 cut(s) 385
AfiI CCNNNNNNNGG 1 cut(s) 130
AgsI TTSAA 5 cut(s) 11, 56, 243, 324, 392
AjnI CCWGG 1 cut(s) 284
AluBI AGCT 1 cut(s) 266
AluI AGCT 1 cut(s) 266
Alw26I GTCTC 1 cut(s) 77
AlwI GGATC 3 cut(s) 275, 288, 368
AsuHPI GGTGA 3 cut(s) 158, 301, 317
BamHI GGATCC 1 cut(s) 280
BauI CACGAG 1 cut(s) 258
BccI CCATC 2 cut(s) 149, 302
BciT130I CCWGG 1 cut(s) 286
BcoDI GTCTC 1 cut(s) 77
BfaI CTAG 1 cut(s) 125
BfmI CTRYAG 1 cut(s) 87
Bme1390I CCNGG 1 cut(s) 286
BmiI GGNNCC 1 cut(s) 282
BmrFI CCNGG 1 cut(s) 286
BmsI GCATC 2 cut(s) 28, 340
BpuEI CTTGAG 1 cut(s) 29
BsaJI CCNNGG 2 cut(s) 284, 285
Bsc4I CCNNNNNNNGG 1 cut(s) 130
BseBI CCWGG 1 cut(s) 286
BseDI CCNNGG 2 cut(s) 284, 285
BseGI GGATG 1 cut(s) 120
BseLI CCNNNNNNNGG 1 cut(s) 130
BslI CCNNNNNNNGG 1 cut(s) 130
BsmAI GTCTC 1 cut(s) 77
Bsp143I GATC 2 cut(s) 280, 373
BspLI GGNNCC 1 cut(s) 282
BspPI GGATC 3 cut(s) 275, 288, 368
BssECI CCNNGG 2 cut(s) 284, 285
BssMI GATC 2 cut(s) 280, 373
BssSI CACGAG 1 cut(s) 258
Bst2BI CACGAG 1 cut(s) 258
Bst2UI CCWGG 1 cut(s) 286
Bst4CI ACNGT 2 cut(s) 91, 249
Bst6I CTCTTC 1 cut(s) 373
BstF5I GGATG 1 cut(s) 120
BstKTI GATC 2 cut(s) 283, 376
BstMAI GTCTC 1 cut(s) 77
BstMBI GATC 2 cut(s) 280, 373
BstMWI GCNNNNNNNGC 1 cut(s) 335
BstNI CCWGG 1 cut(s) 286
BstSCI CCNGG 1 cut(s) 284
BstSFI CTRYAG 1 cut(s) 87
BstX2I RGATCY 1 cut(s) 280
BstYI RGATCY 1 cut(s) 280
BtsCI GGATG 1 cut(s) 120
Csp6I GTAC 1 cut(s) 384
CspCI CAANNNNNGTGG 2 cut(s) 342, 377
CviAII CATG 1 cut(s) 299
CviJI RGCY 3 cut(s) 179, 266, 329
CviKI_1 RGCY 3 cut(s) 179, 266, 329
CviQI GTAC 1 cut(s) 384
DpnI GATC 2 cut(s) 282, 375
DpnII GATC 2 cut(s) 280, 373
Eam1104I CTCTTC 1 cut(s) 373
EarI CTCTTC 1 cut(s) 373
EcoRII CCWGG 1 cut(s) 284
FaeI CATG 1 cut(s) 302
FaiI YATR 7 cut(s) 108, 209, 211, 276, 300, 348, 380
FatI CATG 1 cut(s) 298
FokI GGATG 1 cut(s) 107
FspBI CTAG 1 cut(s) 125
Hin1II CATG 1 cut(s) 302
HinfI GANTC 2 cut(s) 312, 365
HphI GGTGA 3 cut(s) 158, 301, 317
Hpy166II GTNNAC 1 cut(s) 364
Hpy188I TCNGA 1 cut(s) 373
Hpy8I GTNNAC 1 cut(s) 364
HpyAV CCTTC 1 cut(s) 143
HpyCH4III ACNGT 2 cut(s) 91, 249
HpyCH4V TGCA 3 cut(s) 35, 41, 353
HpyF10VI GCNNNNNNNGC 1 cut(s) 335
Hsp92II CATG 1 cut(s) 302
Kzo9I GATC 2 cut(s) 280, 373
LpnPI CCDG 4 cut(s) 271, 298, 324, 348
LweI GCATC 2 cut(s) 28, 340
MaeI CTAG 1 cut(s) 125
MaeIII GTNAC 1 cut(s) 289
MalI GATC 2 cut(s) 282, 375
MboI GATC 2 cut(s) 280, 373
MboII GAAGA 3 cut(s) 83, 180, 360
MflI RGATCY 1 cut(s) 280
MluCI AATT 1 cut(s) 357
MlyI GAGTC 2 cut(s) 321, 359
MnlI CCTC 3 cut(s) 162, 267, 271
MseI TTAA 1 cut(s) 21
MslI CAYNNNNRTG 1 cut(s) 303
MspR9I CCNGG 1 cut(s) 286
MvaI CCWGG 1 cut(s) 286
MwoI GCNNNNNNNGC 1 cut(s) 335
NdeII GATC 2 cut(s) 280, 373
NlaIII CATG 1 cut(s) 302
NlaIV GGNNCC 1 cut(s) 282
NmuCI GTSAC 1 cut(s) 289
PasI CCCWGGG 1 cut(s) 285
PleI GAGTC 2 cut(s) 320, 359
PpsI GAGTC 2 cut(s) 320, 359
Psp6I CCWGG 1 cut(s) 284
PspGI CCWGG 1 cut(s) 284
PspN4I GGNNCC 1 cut(s) 282
PsuI RGATCY 1 cut(s) 280
RsaI GTAC 1 cut(s) 385
RsaNI GTAC 1 cut(s) 384
RseI CAYNNNNRTG 1 cut(s) 303
SaqAI TTAA 1 cut(s) 21
Sau3AI GATC 2 cut(s) 280, 373
SchI GAGTC 2 cut(s) 321, 359
ScrFI CCNGG 1 cut(s) 286
SetI ASST 4 cut(s) 107, 117, 268, 385
SfaNI GCATC 2 cut(s) 28, 340
SfcI CTRYAG 1 cut(s) 87
SmiMI CAYNNNNRTG 1 cut(s) 303
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 1 cut(s) 357
SspI AATATT 1 cut(s) 395
SspMI CTAG 1 cut(s) 125
StyD4I CCNGG 1 cut(s) 284
TaaI ACNGT 2 cut(s) 91, 249
TaqI TCGA 1 cut(s) 195
TasI AATT 1 cut(s) 357
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TseFI GTSAC 1 cut(s) 289
Tsp45I GTSAC 1 cut(s) 289
TspDTI ATGAA 1 cut(s) 84
XcmI CCANNNNNNNNNTGG 1 cut(s) 305
XspI CTAG 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.