pycom06g19230

Carbon catabolite repressor protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0003003
Physical Location & Seq
Reverse (-)
22686663 .. 22690168
3506 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1356 bp
ATGGAAGAAACCGGCGATGTACGGCGGCGTAAGCGCAAGCAACATTCACACAACAAAGAAGCCGCAGAGAACCACCCTCACTCTTCGCCTCACAAAAGGCGGCGGCGCAGAAGAGTGAAGTCCGAATCTAAAACCCTAACCCGAGAATCCGACCCGCCACCGCCGCCGTCGTCATCCAGGTTTCAATTATTATCGCCTTCGTATCGGAAGCACACGAAGCGGCAACCGAGTCGAACCGAGACAGAGAGCTCCCGGGAGTGGGCTTCCTCTCCCTGCAGCTCTTCTGCTTCCACCTATGGAGAGAAATTTGTGGTTGTTTCTTATAACATTCTGGGTGTGGAAAATGCATCCAAGCACCCAGAGTTGTACTACAAAGTCCCACCCATGTTCTTGGAGTGGAACCGCCGCAGGAAACTTATTCGGAAGGAGATCAATCGCTACAATGCTAGCATCATGTGCTTGCAGGAGGTTGATCGTTTTGATGATTTAGCTCATCTTTTCCGGAAAGATGGGTTCGAAGGTGTTTATAAGGCACGCACGGGTGAAGCAAATGATGGATGCGCCATATTTTGGAAAAAGGAAATGTTTAGCCTTTTACATGAAGAAAATATAGAGTTCCAAAGTTTTGGACTTCGTAACAATGTTGCTCAACTTTGTGTATTAAAGAGTAACCAAAATCAATTAGAGTCAGACATACCTCCTGGAACGAGTCCATACACCCAAAGTAGAAGCTTAGTGATTGGGAATACACATCTACTATTCAACCCAAAACGTGGAGATATCAAGCTAGGGCAGGTCCGACTGTTTCTTAAAAAAGCTTACGAGCTATCACAAGAGTGGGGCATCATCCCTGTTATAATTTCCGGAGATCTAAATAGCATCCCACAGAGTCCTATGTACCATTTCCTAGCTTCTTCTGAGTTGGACATCCGACTACATGACCGCAGAGGTATATCCGGACAGTTTGTCCTGAACTCGAATCACAGAGGCTTCAGATTTCAGAATGAGCAAGCAAGCAGGCCATTTTCATATATATGGAGCGAGGAAGAAGTAAGGCTTGCAACTGGCAGTGAAGTGGTCACTTGTCTTCGGCACGATTTGAAGCTTTGCAGTGCATACCTGGGAGTACCTGGTAGTTGTAGAACCAGAGATAGGTCTGGAGAACCCTTGGCAACGTCATACCACTCCGAGTTTATGGGAACTGTTGATTATATTTGGCATACTGGGGAACTTGTTCCTGTCAGAGTTCTTGAAACCTTACCAGTCAATGCTTTAAGAAGAGATGGAGGACTCCCCAGCAAGCAATGGGGCAGCGATCATCTTGCCGTTGTATGCGAACTGGCTTTTCGTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

452

Amino Acids

51.87

Weight (kDa)

9.48

Isoelectric Point (pI)

64.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 107 - 440 1.1e-15 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 324, 528, 857
Acc36I ACCTGC 1 cut(s) 784
AccB7I CCANNNNNTGG 2 cut(s) 570, 773
AccIII TCCGGA 3 cut(s) 501, 863, 956
AcsI RAATTY 1 cut(s) 305
AcuI CTGAAG 1 cut(s) 976
AfaI GTAC 4 cut(s) 21, 368, 899, 1128
AfiI CCNNNNNNNGG 5 cut(s) 258, 259, 570, 773, 1152
AgsI TTSAA 4 cut(s) 185, 763, 1102, 1253
AhdI GACNNNNNGTC 1 cut(s) 965
AjnI CCWGG 4 cut(s) 176, 700, 1119, 1129
AleI CACNNNNGTG 1 cut(s) 835
AluBI AGCT 9 cut(s) 249, 279, 491, 732, 787, 818, 826, 911, 1105
AluI AGCT 9 cut(s) 249, 279, 491, 732, 787, 818, 826, 911, 1105
Alw21I GWGCWC 1 cut(s) 251
Alw26I GTCTC 1 cut(s) 233
Ama87I CYCGRG 2 cut(s) 141, 252
Aor13HI TCCGGA 3 cut(s) 501, 863, 956
AoxI GGCC 1 cut(s) 1019
ApeKI GCWGC 2 cut(s) 276, 1311
ApoI RAATTY 1 cut(s) 305
Asp700I GAANNNNTTC 1 cut(s) 1233
AspLEI GCGC 3 cut(s) 36, 108, 563
AspS9I GGNCC 1 cut(s) 796
AsuC2I CCSGG 2 cut(s) 253, 254
AsuHPI GGTGA 1 cut(s) 554
AsuII TTCGAA 1 cut(s) 516
AsuNHI GCTAGC 1 cut(s) 446
AvaI CYCGRG 2 cut(s) 141, 252
AvaII GGWCC 1 cut(s) 796
BanII GRGCYC 1 cut(s) 251
BbsI GAAGAC 1 cut(s) 1079
Bbv12I GWGCWC 1 cut(s) 251
BbvI GCAGC 2 cut(s) 288, 1323
BccI CCATC 3 cut(s) 503, 548, 1277
BceAI ACGGC 3 cut(s) 38, 151, 1310
BcgI CGANNNNNNTGC 4 cut(s) 212, 246, 1304, 1338
BciT130I CCWGG 4 cut(s) 178, 702, 1121, 1131
BcnI CCSGG 2 cut(s) 253, 254
BcoDI GTCTC 1 cut(s) 233
BfaI CTAG 3 cut(s) 447, 788, 908
BfmI CTRYAG 1 cut(s) 274
BfuAI ACCTGC 1 cut(s) 784
BglII AGATCT 1 cut(s) 868
BisI GCNGC 9 cut(s) 26, 63, 101, 104, 164, 221, 277, 406, 1312
BlsI GCNGC 9 cut(s) 27, 64, 102, 105, 165, 222, 278, 407, 1313
Bme1390I CCNGG 6 cut(s) 178, 253, 254, 702, 1121, 1131
Bme18I GGWCC 1 cut(s) 796
BmeRI GACNNNNNGTC 1 cut(s) 965
BmeT110I CYCGRG 2 cut(s) 141, 252
BmgT120I GGNCC 1 cut(s) 796
BmiI GGNNCC 1 cut(s) 401
BmrFI CCNGG 6 cut(s) 178, 253, 254, 702, 1121, 1131
BmrI ACTGGG 1 cut(s) 1233
BmsI GCATC 5 cut(s) 356, 459, 548, 852, 888
BmtI GCTAGC 1 cut(s) 450
BmuI ACTGGG 1 cut(s) 1233
BpiI GAAGAC 1 cut(s) 1079
BpmI CTGGAG 1 cut(s) 1179
Bpu14I TTCGAA 1 cut(s) 516
BpuMI CCSGG 2 cut(s) 253, 254
BsaJI CCNNGG 3 cut(s) 252, 1120, 1167
BsaWI WCCGGW 3 cut(s) 501, 863, 956
Bsc4I CCNNNNNNNGG 5 cut(s) 258, 259, 570, 773, 1152
Bse118I RCCGGY 1 cut(s) 11
Bse1I ACTGG 4 cut(s) 1069, 1228, 1262, 1344
Bse3DI GCAATG 1 cut(s) 1310
BseAI TCCGGA 3 cut(s) 501, 863, 956
BseBI CCWGG 4 cut(s) 178, 702, 1121, 1131
BseDI CCNNGG 3 cut(s) 252, 1120, 1167
BseGI GGATG 6 cut(s) 173, 347, 563, 846, 879, 927
BseLI CCNNNNNNNGG 5 cut(s) 258, 259, 570, 773, 1152
BseMI GCAATG 1 cut(s) 1310
BseMII CTCAG 1 cut(s) 909
BseNI ACTGG 4 cut(s) 1069, 1228, 1262, 1344
BseXI GCAGC 2 cut(s) 288, 1323
BseYI CCCAGC 1 cut(s) 1295
BshFI GGCC 1 cut(s) 1021
BsiHKAI GWGCWC 1 cut(s) 251
BsiHKCI CYCGRG 2 cut(s) 141, 252
BsiSI CCGG 5 cut(s) 12, 253, 502, 864, 957
BslFI GGGAC 1 cut(s) 362
BslI CCNNNNNNNGG 5 cut(s) 258, 259, 570, 773, 1152
BsmAI GTCTC 1 cut(s) 233
BsmFI GGGAC 1 cut(s) 362
BsnI GGCC 1 cut(s) 1021
BsoBI CYCGRG 2 cut(s) 141, 252
Bsp119I TTCGAA 1 cut(s) 516
Bsp1286I GDGCHC 1 cut(s) 251
Bsp13I TCCGGA 3 cut(s) 501, 863, 956
Bsp143I GATC 4 cut(s) 429, 472, 868, 1315
BspANI GGCC 1 cut(s) 1021
BspCNI CTCAG 1 cut(s) 910
BspEI TCCGGA 3 cut(s) 501, 863, 956
BspLI GGNNCC 1 cut(s) 401
BspMAI CTGCAG 1 cut(s) 278
BspMI ACCTGC 1 cut(s) 784
BspOI GCTAGC 1 cut(s) 450
BspQI GCTCTTC 1 cut(s) 286
BspT104I TTCGAA 1 cut(s) 516
BsrDI GCAATG 1 cut(s) 1310
BsrFI RCCGGY 1 cut(s) 11
BsrI ACTGG 4 cut(s) 1069, 1228, 1262, 1344
BssAI RCCGGY 1 cut(s) 11
BssECI CCNNGG 3 cut(s) 252, 1120, 1167
BssMI GATC 4 cut(s) 429, 472, 868, 1315
BssT1I CCWWGG 1 cut(s) 1167
Bst2UI CCWGG 4 cut(s) 178, 702, 1121, 1131
Bst4CI ACNGT 3 cut(s) 804, 963, 1204
Bst6I CTCTTC 4 cut(s) 88, 106, 286, 1273
BstAPI GCANNNNNTGC 1 cut(s) 456
BstBI TTCGAA 1 cut(s) 516
BstC8I GCNNGC 9 cut(s) 38, 448, 461, 535, 1011, 1015, 1019, 1059, 1301
BstDEI CTNAG 2 cut(s) 733, 918
BstF5I GGATG 6 cut(s) 173, 347, 563, 846, 879, 927
BstHHI GCGC 3 cut(s) 36, 108, 563
BstKTI GATC 4 cut(s) 432, 475, 871, 1318
BstMAI GTCTC 1 cut(s) 233
BstMBI GATC 4 cut(s) 429, 472, 868, 1315
BstMWI GCNNNNNNNGC 4 cut(s) 31, 163, 217, 456
BstNI CCWGG 4 cut(s) 178, 702, 1121, 1131
BstSCI CCNGG 6 cut(s) 176, 251, 252, 700, 1119, 1129
BstSFI CTRYAG 1 cut(s) 274
BstV1I GCAGC 2 cut(s) 288, 1323
BstV2I GAAGAC 1 cut(s) 1079
BstX2I RGATCY 1 cut(s) 868
BstXI CCANNNNNNTGG 2 cut(s) 391, 626
BstYI RGATCY 1 cut(s) 868
BsuRI GGCC 1 cut(s) 1021
BtgZI GCGATG 1 cut(s) 30
BtsCI GGATG 6 cut(s) 173, 347, 563, 846, 879, 927
BtsI GCAGTG 2 cut(s) 1075, 1117
BtsIMutI CAGTG 2 cut(s) 1075, 1117
BveI ACCTGC 1 cut(s) 784
Cac8I GCNNGC 9 cut(s) 38, 448, 461, 535, 1011, 1015, 1019, 1059, 1301
CfoI GCGC 3 cut(s) 36, 108, 563
Cfr10I RCCGGY 1 cut(s) 11
Cfr13I GGNCC 1 cut(s) 796
Cfr9I CCCGGG 1 cut(s) 252
CsiI ACCWGGT 1 cut(s) 1129
Csp6I GTAC 4 cut(s) 20, 367, 898, 1127
CviAII CATG 4 cut(s) 385, 454, 599, 938
CviQI GTAC 4 cut(s) 20, 367, 898, 1127
DdeI CTNAG 2 cut(s) 733, 918
DpnI GATC 4 cut(s) 431, 474, 870, 1317
DpnII GATC 4 cut(s) 429, 472, 868, 1315
DriI GACNNNNNGTC 1 cut(s) 965
Eam1104I CTCTTC 4 cut(s) 88, 106, 286, 1273
Eam1105I GACNNNNNGTC 1 cut(s) 965
EarI CTCTTC 4 cut(s) 88, 106, 286, 1273
Ecl136II GAGCTC 1 cut(s) 249
Eco130I CCWWGG 1 cut(s) 1167
Eco24I GRGCYC 1 cut(s) 251
Eco32I GATATC 1 cut(s) 781
Eco47I GGWCC 1 cut(s) 796
Eco53kI GAGCTC 1 cut(s) 249
Eco57I CTGAAG 1 cut(s) 976
Eco88I CYCGRG 2 cut(s) 141, 252
EcoICRI GAGCTC 1 cut(s) 249
EcoRII CCWGG 4 cut(s) 176, 700, 1119, 1129
EcoRV GATATC 1 cut(s) 781
EcoT14I CCWWGG 1 cut(s) 1167
EcoT22I ATGCAT 1 cut(s) 349
EcoT38I GRGCYC 1 cut(s) 251
ErhI CCWWGG 1 cut(s) 1167
FaeI CATG 4 cut(s) 388, 457, 602, 941
FalI AAGNNNNNCTT 2 cut(s) 1041, 1073
FaqI GGGAC 1 cut(s) 362
FatI CATG 4 cut(s) 384, 453, 598, 937
FauI CCCGC 1 cut(s) 162
Fnu4HI GCNGC 9 cut(s) 26, 63, 101, 104, 164, 221, 277, 406, 1312
FokI GGATG 6 cut(s) 160, 334, 570, 833, 866, 914
FriOI GRGCYC 1 cut(s) 251
Fsp4HI GCNGC 9 cut(s) 26, 63, 101, 104, 164, 221, 277, 406, 1312
FspBI CTAG 3 cut(s) 447, 788, 908
GlaI GCGC 3 cut(s) 35, 107, 562
GluI GCNGC 9 cut(s) 26, 63, 101, 104, 164, 221, 277, 406, 1312
GsaI CCCAGC 1 cut(s) 1299
GsuI CTGGAG 1 cut(s) 1179
HaeIII GGCC 1 cut(s) 1021
HapII CCGG 5 cut(s) 12, 253, 502, 864, 957
HhaI GCGC 3 cut(s) 36, 108, 563
Hin1II CATG 4 cut(s) 388, 457, 602, 941
Hin6I GCGC 3 cut(s) 34, 106, 561
HinP1I GCGC 3 cut(s) 34, 106, 561
HindIII AAGCTT 3 cut(s) 730, 816, 1103
HinfI GANTC 8 cut(s) 125, 146, 229, 686, 709, 889, 979, 1290
HpaII CCGG 5 cut(s) 12, 253, 502, 864, 957
HphI GGTGA 1 cut(s) 554
Hpy188III TCNNGA 6 cut(s) 502, 864, 957, 970, 1158, 1250
Hpy99I CGWCG 1 cut(s) 172
HpyAV CCTTC 3 cut(s) 207, 418, 512
HpyCH4III ACNGT 3 cut(s) 804, 963, 1204
HpyCH4IV ACGT 2 cut(s) 772, 1175
HpyCH4V TGCA 6 cut(s) 276, 347, 463, 1061, 1110, 1115
HpyF10VI GCNNNNNNNGC 4 cut(s) 31, 163, 217, 456
HpyF3I CTNAG 2 cut(s) 733, 918
HpySE526I ACGT 2 cut(s) 772, 1175
Hsp92II CATG 4 cut(s) 388, 457, 602, 941
HspAI GCGC 3 cut(s) 34, 106, 561
Kpn2I TCCGGA 3 cut(s) 501, 863, 956
Kzo9I GATC 4 cut(s) 429, 472, 868, 1315
LguI GCTCTTC 1 cut(s) 286
LmnI GCTCC 2 cut(s) 254, 1038
Lsp1109I GCAGC 2 cut(s) 288, 1323
LweI GCATC 5 cut(s) 356, 459, 548, 852, 888
MabI ACCWGGT 1 cut(s) 1129
MaeI CTAG 3 cut(s) 447, 788, 908
MaeII ACGT 2 cut(s) 772, 1175
MaeIII GTNAC 3 cut(s) 635, 668, 1078
MalI GATC 4 cut(s) 431, 474, 870, 1317
MboI GATC 4 cut(s) 429, 472, 868, 1315
MboII GAAGA 9 cut(s) 17, 75, 123, 273, 614, 906, 1058, 1079, 1290
MflI RGATCY 1 cut(s) 868
MhlI GDGCHC 1 cut(s) 251
MluCI AATT 4 cut(s) 185, 305, 680, 858
MlyI GAGTC 5 cut(s) 238, 695, 718, 898, 1284
MmeI TCCRAC 4 cut(s) 174, 823, 903, 955
MnlI CCTC 9 cut(s) 87, 99, 277, 460, 708, 941, 980, 1036, 1280
Mph1103I ATGCAT 1 cut(s) 349
MroI TCCGGA 3 cut(s) 501, 863, 956
MroXI GAANNNNTTC 1 cut(s) 1233
MseI TTAA 3 cut(s) 662, 810, 1274
MslI CAYNNNNRTG 2 cut(s) 835, 1033
MspI CCGG 5 cut(s) 12, 253, 502, 864, 957
MspR9I CCNGG 6 cut(s) 178, 253, 254, 702, 1121, 1131
MvaI CCWGG 4 cut(s) 178, 702, 1121, 1131
MwoI GCNNNNNNNGC 4 cut(s) 31, 163, 217, 456
NciI CCSGG 2 cut(s) 253, 254
NdeII GATC 4 cut(s) 429, 472, 868, 1315
NheI GCTAGC 1 cut(s) 446
NlaIII CATG 4 cut(s) 388, 457, 602, 941
NlaIV GGNNCC 1 cut(s) 401
NmuCI GTSAC 1 cut(s) 1078
NsiI ATGCAT 1 cut(s) 349
NspV TTCGAA 1 cut(s) 516
OliI CACNNNNGTG 1 cut(s) 835
PciSI GCTCTTC 1 cut(s) 286
PdmI GAANNNNTTC 1 cut(s) 1233
PfeI GAWTC 3 cut(s) 125, 146, 979
PflMI CCANNNNNTGG 2 cut(s) 570, 773
PfoI TCCNGGA 1 cut(s) 700
PkrI GCNGC 9 cut(s) 27, 64, 102, 105, 165, 222, 278, 407, 1313
PleI GAGTC 5 cut(s) 237, 694, 717, 897, 1284
PpsI GAGTC 5 cut(s) 237, 694, 717, 897, 1284
PsiI TTATAA 3 cut(s) 324, 528, 857
Psp124BI GAGCTC 1 cut(s) 251
Psp6I CCWGG 4 cut(s) 176, 700, 1119, 1129
PspFI CCCAGC 1 cut(s) 1295
PspGI CCWGG 4 cut(s) 176, 700, 1119, 1129
PspN4I GGNNCC 1 cut(s) 401
PspPI GGNCC 1 cut(s) 796
PstI CTGCAG 1 cut(s) 278
PsuI RGATCY 1 cut(s) 868
RsaI GTAC 4 cut(s) 21, 368, 899, 1128
RsaNI GTAC 4 cut(s) 20, 367, 898, 1127
RseI CAYNNNNRTG 2 cut(s) 835, 1033
SacI GAGCTC 1 cut(s) 251
SapI GCTCTTC 1 cut(s) 286
SaqAI TTAA 3 cut(s) 662, 810, 1274
SatI GCNGC 9 cut(s) 26, 63, 101, 104, 164, 221, 277, 406, 1312
Sau3AI GATC 4 cut(s) 429, 472, 868, 1315
Sau96I GGNCC 1 cut(s) 796
SchI GAGTC 5 cut(s) 238, 695, 718, 898, 1284
ScrFI CCNGG 6 cut(s) 178, 253, 254, 702, 1121, 1131
SduI GDGCHC 1 cut(s) 251
SexAI ACCWGGT 1 cut(s) 1129
SfaNI GCATC 5 cut(s) 356, 459, 548, 852, 888
SfcI CTRYAG 1 cut(s) 274
SfuI TTCGAA 1 cut(s) 516
SinI GGWCC 1 cut(s) 796
SmaI CCCGGG 1 cut(s) 254
SmiMI CAYNNNNRTG 2 cut(s) 835, 1033
Sse9I AATT 4 cut(s) 185, 305, 680, 858
SspMI CTAG 3 cut(s) 447, 788, 908
SstI GAGCTC 1 cut(s) 251
StyD4I CCNGG 6 cut(s) 176, 251, 252, 700, 1119, 1129
StyI CCWWGG 1 cut(s) 1167
TaaI ACNGT 3 cut(s) 804, 963, 1204
TaiI ACGT 2 cut(s) 775, 1178
TaqI TCGA 3 cut(s) 232, 516, 977
TasI AATT 4 cut(s) 185, 305, 680, 858
TatI WGTACW 1 cut(s) 366
TauI GCSGC 7 cut(s) 28, 65, 103, 106, 166, 223, 408
TfiI GAWTC 3 cut(s) 125, 146, 979
Tru1I TTAA 3 cut(s) 662, 810, 1274
Tru9I TTAA 3 cut(s) 662, 810, 1274
TscAI CASTG 2 cut(s) 1075, 1117
TseFI GTSAC 1 cut(s) 1078
TseI GCWGC 2 cut(s) 276, 1311
Tsp45I GTSAC 1 cut(s) 1078
TspDTI ATGAA 2 cut(s) 615, 1017
TspMI CCCGGG 1 cut(s) 252
TspRI CASTG 2 cut(s) 1075, 1117
Van91I CCANNNNNTGG 2 cut(s) 570, 773
VpaK11BI GGWCC 1 cut(s) 796
XapI RAATTY 1 cut(s) 305
XmaI CCCGGG 1 cut(s) 252
XmnI GAANNNNTTC 1 cut(s) 1233
XspI CTAG 3 cut(s) 447, 788, 908
Zsp2I ATGCAT 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.