pycom07g01040

Dual-specificity RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0010937
Physical Location & Seq
Reverse (-)
860947 .. 863595
2649 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1323 bp
ATGGGCTTGGTCCAACTTGGCCACACTTCCTCTGCTTCATCTTCTTCATCTCGCGCCAAAGTAAAAACCCAGCACAGCACAATGATGACAGCCTCATCAATGTCGCTAATTCAGCGCGTCTGCGCCGTTCCTCTCGCACGCGCTGCCCGCACTCGCGCCATGGCAGCGGCGAGCATGAACCTGTCCACCACCACCACCGCCCCCAAAAGCACCGACCCCCAAGTCCTCCTCGGCTTGTCGGAACCGGAGCTCCAACAGCTCGCAATCGACTTCAGTCAGCAAGCCTACAGAGGGAAGCAGCTCCATCATCTTATTTACAAAAGAAAGATTAAAGATATTCAGGATTTCAATCAGGTGCCTCTGGCATTCAGAAATGAGCTTGAGGAAGCTGGATGGAAAGTTGGTAGGTCACCCATTTACCAGAGCGTCACTGCTGCTGATGGCACCGTCAAGTTATTGATAAAGTTGGAGGATAACAGATTGATTGAAACTGTTGGCATACCAGTTAAAGACGAGAAAGGTGTGATGCGCCTTACAGCGTGTGTTTCATCACAGGTGGGATGCCCTCTTCGTTGCTCTTTTTGTGCCACAGGAAAAGGAGGATTTTCAAGAAACCTCAAGAGGCATGAAATTGTTGAGCAGGTTTTGGCTATAGAGGAAATCTTCAGCCATAGGGTGACTAATGTGGTGTTCATGGGAATGGGTGAACCGATGATGAACCTGAAGGCGGTACTTGAAGCGCATCAGTGTCTAAACAAGGATATTCAAATTGGGCAAAGAATGATCACAATTTCTACCGTTGGGGTTCCAAATACAATTAAAAGGCTAGCATCTCACAAACTTCAGTCGACATTGGCTGTCAGCCTGCATGCTCCTAACCAGAAGCTGAGGGAAACAATTGTGCCTAGTGCAAAGGCATACCCTCTGAGTGCACTGATGAGGGATTGCAGGGACTATTTCACTGAAACCGGTAGACGGGTTTCCTTTGAGTATGCACTCTTAGCTGGAGTTAATGATGCGGTAGACCATGCAGTAGAACTTGCAGAGCTACTCCATGAGTGGGGACGAGGTTCTCATGTGAACTTGATACCATTTAATCCAATAGAAGGCTCTGAGTATAAACGTCCATCCAAGAAAGCGGTACAAGCATTTGCGGCTGCTCTGGAGAACAATAAAGTAACCGTCAGCACACGTCAAACAAGGGGCTTGGATGCAGATGCAGCTTGTGGTCAGCTAAGAAACAAGTTCCAGAAGAGTCCATTGCCCGTTGACTCTGACAACTTACAAACTGAAGAAGATGTTCCAGTTGCAGTTGCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

441

Amino Acids

48.23

Weight (kDa)

9.2

Isoelectric Point (pI)

45.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 183 - 347 4.9e-16 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010937)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39670 AT2G39670
fragaria_vesca FvH4_7g22600
malus_domestica MD07G1012700.v1.1 MD07G1199600.v1.1
prunus_persica Prupe.2G236300_v2.0.a1
pyrus_communis pycom07g01040 pycom07g18640
rosa_chinensis RchiOBHm_Chr1g0367701
rosa_laevigata RLG00000027269
rosa_multiflora Rmu_sc0008470.1_g000011 Rmu_sc0022951.1_g000001 Rmu_sc0027455.1_g000001
rosa_roxburghii Rroxscaffold_4G00289290
rosa_rugosa Rorug01G0336200
rosa_samantha Rh1AG343200 Rh1BG304900 Rh1CG320600 Rh1DG337200
rosa_wichuraiana Rw1G030540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 221
Acc36I ACCTGC 1 cut(s) 631
AccB1I GGYRCC 2 cut(s) 355, 443
AccI GTMKAC 3 cut(s) 848, 973, 1023
AccII CGCG 4 cut(s) 54, 117, 141, 156
AciI CCGC 7 cut(s) 148, 167, 198, 728, 1019, 1139, 1154
AcoI YGGCCR 1 cut(s) 19
AcuI CTGAAG 5 cut(s) 256, 649, 743, 827, 1311
AfaI GTAC 2 cut(s) 732, 1143
AfiI CCNNNNNNNGG 5 cut(s) 291, 727, 975, 1060, 1106
AflIII ACRYGT 1 cut(s) 1190
AgeI ACCGGT 1 cut(s) 968
AgsI TTSAA 5 cut(s) 349, 488, 609, 737, 767
AjiI CACGTC 1 cut(s) 1193
AloI GAACNNNNNNTCC 2 cut(s) 234, 266
Alw21I GWGCWC 2 cut(s) 252, 934
Alw44I GTGCAC 1 cut(s) 930
AlwNI CAGNNNCTG 1 cut(s) 886
AoxI GGCC 1 cut(s) 19
ApaLI GTGCAC 1 cut(s) 930
ApeKI GCWGC 6 cut(s) 143, 164, 298, 434, 1157, 1220
AsiGI ACCGGT 1 cut(s) 968
Asp700I GAANNNNTTC 1 cut(s) 1299
AspLEI GCGC 7 cut(s) 56, 117, 125, 143, 158, 531, 742
AspS9I GGNCC 1 cut(s) 10
AsuHPI GGTGA 3 cut(s) 402, 688, 716
AsuNHI GCTAGC 1 cut(s) 826
AvaII GGWCC 1 cut(s) 10
BaeGI GKGCMC 1 cut(s) 934
BalI TGGCCA 1 cut(s) 21
BanI GGYRCC 2 cut(s) 355, 443
BanII GRGCYC 1 cut(s) 252
Bbv12I GWGCWC 2 cut(s) 252, 934
BbvCI CCTCAGC 1 cut(s) 887
BbvI GCAGC 6 cut(s) 130, 176, 310, 421, 1144, 1232
BccI CCATC 4 cut(s) 312, 387, 434, 1135
BceAI ACGGC 1 cut(s) 110
BclI TGATCA 1 cut(s) 783
BfaI CTAG 2 cut(s) 827, 906
BfmI CTRYAG 2 cut(s) 286, 651
BfuAI ACCTGC 1 cut(s) 631
BisI GCNGC 8 cut(s) 144, 165, 168, 299, 435, 1155, 1158, 1221
BlsI GCNGC 8 cut(s) 145, 166, 169, 300, 436, 1156, 1159, 1222
Bme18I GGWCC 1 cut(s) 10
BmgBI CACGTC 1 cut(s) 1193
BmgT120I GGNCC 1 cut(s) 10
BmiI GGNNCC 4 cut(s) 243, 357, 445, 807
BmsI GCATC 7 cut(s) 516, 551, 751, 839, 1006, 1201, 1207
BmtI GCTAGC 1 cut(s) 830
BoxI GACNNNNGTC 1 cut(s) 273
BpmI CTGGAG 2 cut(s) 1026, 1184
Bpu10I CCTNAGC 1 cut(s) 887
BpuEI CTTGAG 2 cut(s) 401, 602
BsaBI GATNNNNATC 1 cut(s) 348
BsaJI CCNNGG 2 cut(s) 159, 229
BsaWI WCCGGW 2 cut(s) 244, 968
BsaXI ACNNNNNCTCC 2 cut(s) 234, 264
Bsc4I CCNNNNNNNGG 5 cut(s) 291, 727, 975, 1060, 1106
Bse118I RCCGGY 1 cut(s) 968
Bse1I ACTGG 2 cut(s) 503, 1304
Bse3DI GCAATG 1 cut(s) 1259
Bse8I GATNNNNATC 1 cut(s) 348
BseDI CCNNGG 2 cut(s) 159, 229
BseGI GGATG 4 cut(s) 398, 566, 1127, 1216
BseJI GATNNNNATC 1 cut(s) 348
BseLI CCNNNNNNNGG 5 cut(s) 291, 727, 975, 1060, 1106
BseMI GCAATG 1 cut(s) 1259
BseMII CTCAG 3 cut(s) 878, 917, 1104
BseNI ACTGG 2 cut(s) 503, 1304
BseRI GAGGAG 1 cut(s) 218
BseSI GKGCMC 1 cut(s) 934
BseXI GCAGC 6 cut(s) 130, 176, 310, 421, 1144, 1232
BseYI CCCAGC 1 cut(s) 69
Bsh1236I CGCG 4 cut(s) 54, 117, 141, 156
BshFI GGCC 1 cut(s) 21
BshNI GGYRCC 2 cut(s) 355, 443
BshTI ACCGGT 1 cut(s) 968
BsiHKAI GWGCWC 2 cut(s) 252, 934
BsiSI CCGG 2 cut(s) 245, 969
BslFI GGGAC 2 cut(s) 965, 1077
BslI CCNNNNNNNGG 5 cut(s) 291, 727, 975, 1060, 1106
BsmFI GGGAC 2 cut(s) 965, 1077
BsmI GAATGC 1 cut(s) 365
BsnI GGCC 1 cut(s) 21
Bsp1286I GDGCHC 2 cut(s) 252, 934
Bsp143I GATC 1 cut(s) 783
Bsp19I CCATGG 1 cut(s) 159
BspACI CCGC 7 cut(s) 148, 167, 198, 728, 1019, 1139, 1154
BspANI GGCC 1 cut(s) 21
BspCNI CTCAG 3 cut(s) 879, 918, 1105
BspFNI CGCG 4 cut(s) 54, 117, 141, 156
BspLI GGNNCC 4 cut(s) 243, 357, 445, 807
BspMI ACCTGC 1 cut(s) 631
BspOI GCTAGC 1 cut(s) 830
BspT107I GGYRCC 2 cut(s) 355, 443
BsrDI GCAATG 1 cut(s) 1259
BsrFI RCCGGY 1 cut(s) 968
BsrI ACTGG 2 cut(s) 503, 1304
BssAI RCCGGY 1 cut(s) 968
BssECI CCNNGG 2 cut(s) 159, 229
BssMI GATC 1 cut(s) 783
BssT1I CCWWGG 1 cut(s) 159
Bst4CI ACNGT 4 cut(s) 448, 493, 799, 1183
Bst6I CTCTTC 2 cut(s) 573, 1247
BstAPI GCANNNNNTGC 1 cut(s) 143
BstC8I GCNNGC 8 cut(s) 139, 148, 172, 261, 282, 828, 866, 870
BstDEI CTNAG 5 cut(s) 887, 926, 1000, 1113, 1235
BstDSI CCRYGG 1 cut(s) 159
BstEII GGTNACC 1 cut(s) 408
BstF5I GGATG 4 cut(s) 398, 566, 1127, 1216
BstFNI CGCG 4 cut(s) 54, 117, 141, 156
BstHHI GCGC 7 cut(s) 56, 117, 125, 143, 158, 531, 742
BstKTI GATC 1 cut(s) 786
BstMBI GATC 1 cut(s) 783
BstMWI GCNNNNNNNGC 9 cut(s) 112, 143, 147, 164, 256, 1001, 1145, 1154, 1220
BstNSI RCATGY 2 cut(s) 872, 1320
BstPAI GACNNNNGTC 1 cut(s) 273
BstPI GGTNACC 1 cut(s) 408
BstSFI CTRYAG 2 cut(s) 286, 651
BstSLI GKGCMC 1 cut(s) 934
BstUI CGCG 4 cut(s) 54, 117, 141, 156
BstV1I GCAGC 6 cut(s) 130, 176, 310, 421, 1144, 1232
BsuRI GGCC 1 cut(s) 21
BtgI CCRYGG 1 cut(s) 159
BtrI CACGTC 1 cut(s) 1193
BtsCI GGATG 4 cut(s) 398, 566, 1127, 1216
BtsI GCAGTG 1 cut(s) 429
BtsIMutI CAGTG 4 cut(s) 429, 752, 932, 960
BveI ACCTGC 1 cut(s) 631
Cac8I GCNNGC 8 cut(s) 139, 148, 172, 261, 282, 828, 866, 870
CaiI CAGNNNCTG 1 cut(s) 886
CfoI GCGC 7 cut(s) 56, 117, 125, 143, 158, 531, 742
Cfr10I RCCGGY 1 cut(s) 968
Cfr13I GGNCC 1 cut(s) 10
CseI GACGC 2 cut(s) 106, 415
Csp6I GTAC 2 cut(s) 731, 1142
CspAI ACCGGT 1 cut(s) 968
CviAII CATG 9 cut(s) 160, 175, 626, 694, 869, 1028, 1055, 1076, 1317
CviQI GTAC 2 cut(s) 731, 1142
DdeI CTNAG 5 cut(s) 887, 926, 1000, 1113, 1235
DpnI GATC 1 cut(s) 785
DpnII GATC 1 cut(s) 783
DrdI GACNNNNNNGTC 1 cut(s) 221
DseDI GACNNNNNNGTC 1 cut(s) 221
EaeI YGGCCR 1 cut(s) 19
Eam1104I CTCTTC 2 cut(s) 573, 1247
EarI CTCTTC 2 cut(s) 573, 1247
Ecl136II GAGCTC 1 cut(s) 250
Eco130I CCWWGG 1 cut(s) 159
Eco24I GRGCYC 1 cut(s) 252
Eco47I GGWCC 1 cut(s) 10
Eco53kI GAGCTC 1 cut(s) 250
Eco57I CTGAAG 5 cut(s) 256, 649, 743, 827, 1311
Eco91I GGTNACC 1 cut(s) 408
EcoICRI GAGCTC 1 cut(s) 250
EcoO65I GGTNACC 1 cut(s) 408
EcoT14I CCWWGG 1 cut(s) 159
EcoT38I GRGCYC 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 9 cut(s) 163, 178, 629, 697, 872, 1031, 1058, 1079, 1320
FaqI GGGAC 2 cut(s) 965, 1077
FatI CATG 9 cut(s) 159, 174, 625, 693, 868, 1027, 1054, 1075, 1316
FauI CCCGC 1 cut(s) 155
FbaI TGATCA 1 cut(s) 783
FblI GTMKAC 3 cut(s) 848, 973, 1023
Fnu4HI GCNGC 8 cut(s) 144, 165, 168, 299, 435, 1155, 1158, 1221
FokI GGATG 4 cut(s) 405, 573, 1114, 1223
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 8 cut(s) 144, 165, 168, 299, 435, 1155, 1158, 1221
FspBI CTAG 2 cut(s) 827, 906
GlaI GCGC 7 cut(s) 55, 116, 124, 142, 157, 530, 741
GluI GCNGC 8 cut(s) 144, 165, 168, 299, 435, 1155, 1158, 1221
GsaI CCCAGC 1 cut(s) 73
GsuI CTGGAG 2 cut(s) 1026, 1184
HaeIII GGCC 1 cut(s) 21
HapII CCGG 2 cut(s) 245, 969
HgaI GACGC 2 cut(s) 106, 415
HhaI GCGC 7 cut(s) 56, 117, 125, 143, 158, 531, 742
Hin1II CATG 9 cut(s) 163, 178, 629, 697, 872, 1031, 1058, 1079, 1320
Hin6I GCGC 7 cut(s) 54, 115, 123, 141, 156, 529, 740
HinP1I GCGC 7 cut(s) 54, 115, 123, 141, 156, 529, 740
HincII GTYRAC 2 cut(s) 849, 1270
HindII GTYRAC 2 cut(s) 849, 1270
HinfI GANTC 2 cut(s) 1255, 1271
HpaII CCGG 2 cut(s) 245, 969
HphI GGTGA 3 cut(s) 402, 688, 716
Hpy166II GTNNAC 8 cut(s) 186, 707, 849, 932, 974, 1024, 1081, 1270
Hpy188I TCNGA 5 cut(s) 241, 371, 927, 1114, 1276
Hpy188III TCNNGA 5 cut(s) 341, 609, 619, 1163, 1249
Hpy8I GTNNAC 8 cut(s) 186, 707, 849, 932, 974, 1024, 1081, 1270
HpyAV CCTTC 2 cut(s) 718, 1100
HpyCH4III ACNGT 4 cut(s) 448, 493, 799, 1183
HpyCH4IV ACGT 2 cut(s) 1123, 1192
HpyF10VI GCNNNNNNNGC 9 cut(s) 112, 143, 147, 164, 256, 1001, 1145, 1154, 1220
HpyF3I CTNAG 5 cut(s) 887, 926, 1000, 1113, 1235
HpySE526I ACGT 2 cut(s) 1123, 1192
Hsp92II CATG 9 cut(s) 163, 178, 629, 697, 872, 1031, 1058, 1079, 1320
HspAI GCGC 7 cut(s) 54, 115, 123, 141, 156, 529, 740
Ksp22I TGATCA 1 cut(s) 783
Kzo9I GATC 1 cut(s) 783
LmnI GCTCC 4 cut(s) 247, 255, 306, 877
Lsp1109I GCAGC 6 cut(s) 130, 176, 310, 421, 1144, 1232
LweI GCATC 7 cut(s) 516, 551, 751, 839, 1006, 1201, 1207
MaeI CTAG 2 cut(s) 827, 906
MaeII ACGT 2 cut(s) 1123, 1192
MaeIII GTNAC 4 cut(s) 408, 427, 676, 1177
MalI GATC 1 cut(s) 785
MboI GATC 1 cut(s) 783
MboII GAAGA 7 cut(s) 33, 36, 560, 655, 1264, 1304, 1307
MfeI CAATTG 1 cut(s) 897
MhlI GDGCHC 2 cut(s) 252, 934
MlsI TGGCCA 1 cut(s) 21
MluCI AATT 6 cut(s) 108, 630, 768, 789, 816, 897
MluNI TGGCCA 1 cut(s) 21
MlyI GAGTC 2 cut(s) 1264, 1265
MmeI TCCRAC 4 cut(s) 37, 219, 277, 447
Mox20I TGGCCA 1 cut(s) 21
MroXI GAANNNNTTC 1 cut(s) 1299
MscI TGGCCA 1 cut(s) 21
MseI TTAA 5 cut(s) 330, 507, 819, 1011, 1095
MslI CAYNNNNRTG 2 cut(s) 83, 698
Msp20I TGGCCA 1 cut(s) 21
MspA1I CMGCKG 1 cut(s) 167
MspI CCGG 2 cut(s) 245, 969
MunI CAATTG 1 cut(s) 897
Mva1269I GAATGC 1 cut(s) 365
MvnI CGCG 4 cut(s) 54, 117, 141, 156
MwoI GCNNNNNNNGC 9 cut(s) 112, 143, 147, 164, 256, 1001, 1145, 1154, 1220
NcoI CCATGG 1 cut(s) 159
NdeII GATC 1 cut(s) 783
NheI GCTAGC 1 cut(s) 826
NlaIII CATG 9 cut(s) 163, 178, 629, 697, 872, 1031, 1058, 1079, 1320
NlaIV GGNNCC 4 cut(s) 243, 357, 445, 807
NmeAIII GCCGAG 1 cut(s) 210
NmuCI GTSAC 3 cut(s) 408, 427, 676
NspI RCATGY 2 cut(s) 872, 1320
PaeI GCATGC 1 cut(s) 872
PctI GAATGC 1 cut(s) 365
PdmI GAANNNNTTC 1 cut(s) 1299
PinAI ACCGGT 1 cut(s) 968
PkrI GCNGC 8 cut(s) 145, 166, 169, 300, 436, 1156, 1159, 1222
PleI GAGTC 2 cut(s) 1263, 1265
PpsI GAGTC 2 cut(s) 1263, 1265
PshAI GACNNNNGTC 1 cut(s) 273
Psp124BI GAGCTC 1 cut(s) 252
PspEI GGTNACC 1 cut(s) 408
PspFI CCCAGC 1 cut(s) 69
PspN4I GGNNCC 4 cut(s) 243, 357, 445, 807
PspPI GGNCC 1 cut(s) 10
PstNI CAGNNNCTG 1 cut(s) 886
RsaI GTAC 2 cut(s) 732, 1143
RsaNI GTAC 2 cut(s) 731, 1142
RseI CAYNNNNRTG 2 cut(s) 83, 698
SacI GAGCTC 1 cut(s) 252
SalI GTCGAC 1 cut(s) 847
SaqAI TTAA 5 cut(s) 330, 507, 819, 1011, 1095
SatI GCNGC 8 cut(s) 144, 165, 168, 299, 435, 1155, 1158, 1221
Sau3AI GATC 1 cut(s) 783
Sau96I GGNCC 1 cut(s) 10
SchI GAGTC 2 cut(s) 1264, 1265
SduI GDGCHC 2 cut(s) 252, 934
SfaNI GCATC 7 cut(s) 516, 551, 751, 839, 1006, 1201, 1207
SfcI CTRYAG 2 cut(s) 286, 651
SinI GGWCC 1 cut(s) 10
SmiMI CAYNNNNRTG 2 cut(s) 83, 698
SmlI CTYRAG 2 cut(s) 380, 617
SmoI CTYRAG 2 cut(s) 380, 617
SphI GCATGC 1 cut(s) 872
Sse9I AATT 6 cut(s) 108, 630, 768, 789, 816, 897
SsiI CCGC 7 cut(s) 148, 167, 198, 728, 1019, 1139, 1154
SspMI CTAG 2 cut(s) 827, 906
SstI GAGCTC 1 cut(s) 252
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 4 cut(s) 448, 493, 799, 1183
TaiI ACGT 2 cut(s) 1126, 1195
TaqI TCGA 2 cut(s) 267, 848
TasI AATT 6 cut(s) 108, 630, 768, 789, 816, 897
TauI GCSGC 2 cut(s) 170, 1157
Tru1I TTAA 5 cut(s) 330, 507, 819, 1011, 1095
Tru9I TTAA 5 cut(s) 330, 507, 819, 1011, 1095
TscAI CASTG 4 cut(s) 436, 752, 939, 967
TseFI GTSAC 3 cut(s) 408, 427, 676
TseI GCWGC 6 cut(s) 143, 164, 298, 434, 1157, 1220
Tsp45I GTSAC 3 cut(s) 408, 427, 676
TspDTI ATGAA 7 cut(s) 27, 36, 191, 537, 642, 682, 731
TspRI CASTG 4 cut(s) 436, 752, 939, 967
VneI GTGCAC 1 cut(s) 930
VpaK11BI GGWCC 1 cut(s) 10
XceI RCATGY 2 cut(s) 872, 1320
XmiI GTMKAC 3 cut(s) 848, 973, 1023
XmnI GAANNNNTTC 1 cut(s) 1299
XspI CTAG 2 cut(s) 827, 906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.