pycom07g06770

Ribose-phosphate pyrophosphokinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0008289
Physical Location & Seq
Forward (+)
6088893 .. 6094745
5853 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1065 bp
ATGGCGTCGCTGCTTCACACAGCGTCCCGGTCCTCTCTCGCTTCTCGCGACTATCTACGCCATCGCACTTACCTCCCCAACTACTCTCGCTCTCAAATCCCCATCCTGAATTCCGTGAGGTGTGGTGTGGGAGAGCCTCTCAAGTACGCAAATGGAACGCACGGACTCCTCAGGAGTGAAGATTTGGTGCCGAATTTTTCGGACACGAAAACACATTTGGTGAATGAAATTAGCAAGAATGATACCAGGCTCAGAATCTTTTCCGGCACTGCGAACCCTGCGCTGTCTCAGGAAATTGCTTGCTGCATGGGCCTGGAACTTGGAAGGATTAAGATCGAACTTAGCTTTGCTGATGGAGAGATATATGTTCAGTTGCAAGAGAGTGTAAGAGGGTGTGATGTATATCTTGTGCAACCCACATGTCCTCCTGCAAATGAAAATCTTATGGAGCTTCTTGTCATGATTGATGCTTGTCGGAGGGCTTCAGCCAAGAATATCACTGCTGTGATTCCATATTTTGGCTATGCCAGGGCTGATAGAAAGACTCAAGGGCGTGAATCTATTGCAGCCAAACTTGTTGCAAATTTGATTACAGAAGCAGGTGCAAATCGTGTTCTTGCATGTGATCTTCATTCTGGGCAGTCTATGGGATATTTTGATATTCCTGTGGATCATGTATATGGTCAGCCTGTGATACTTGATTATCTTGCCAGCAAGACTATATACTCTGATGATTTGGTAGTGGTCTCACCCGATGTTGGGGGTGTTGCTAGAGCTCGTGCTTTCGCGAAAAAATTATCTGATGCTCCTTTAGCCATTGTTGATAAAAGACGTCACGGGCACAATGTGGCGGAGGTGATGAATTTGATAGGTGATGTCAAGGGAAAAGTAGCAGTTATGGTGGATGACATGATTGACACAGCTGGGACTATTATGAAAGGTGCTGCTTTACTACACCAAGAGGGTGCCAGGGAAGTCTATGCATGTTGTACACATGCGGTTTTTAGGTATCCCTCCTGCTATAGAGAGGCTGTCGAGTGGTTTGTTTCAAGAGGTGATCGATAA

Protein Analysis

355

Amino Acids

38.87

Weight (kDa)

7.58

Isoelectric Point (pI)

43.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pribosyltran_N PF13793 84 - 201 3.6e-49 N-terminal domain of ribose phosphate pyrophosphokinase
Pribosyltran PF00156 244 - 333 2.2e-17 Phosphoribosyl transferase domain
Pribosyl_synth PF14572 286 - 335 8.4e-12 Phosphoribosyl synthetase-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 590
AatII GACGTC 1 cut(s) 835
Acc36I ACCTGC 1 cut(s) 590
AccB1I GGYRCC 2 cut(s) 187, 965
AccB7I CCANNNNNTGG 1 cut(s) 518
AccII CGCG 2 cut(s) 48, 788
AciI CCGC 2 cut(s) 851, 998
AclWI GGATC 1 cut(s) 678
AcsI RAATTY 4 cut(s) 109, 193, 583, 862
AcuI CTGAAG 1 cut(s) 468
AcyI GRCGYC 2 cut(s) 5, 832
AdeI CACNNNGTG 1 cut(s) 847
AfaI GTAC 2 cut(s) 146, 991
AfiI CCNNNNNNNGG 3 cut(s) 518, 758, 759
AflIII ACRYGT 1 cut(s) 419
AgsI TTSAA 1 cut(s) 1050
AjnI CCWGG 4 cut(s) 245, 312, 527, 968
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AleI CACNNNNGTG 1 cut(s) 503
AluBI AGCT 4 cut(s) 345, 451, 776, 923
AluI AGCT 4 cut(s) 345, 451, 776, 923
Alw21I GWGCWC 1 cut(s) 778
Alw26I GTCTC 2 cut(s) 291, 751
AlwI GGATC 1 cut(s) 678
AoxI GGCC 1 cut(s) 310
ApeKI GCWGC 4 cut(s) 10, 303, 566, 944
ApoI RAATTY 4 cut(s) 109, 193, 583, 862
Asp700I GAANNNNTTC 1 cut(s) 259
AspLEI GCGC 1 cut(s) 283
AspS9I GGNCC 2 cut(s) 30, 310
AsuC2I CCSGG 1 cut(s) 28
AsuHPI GGTGA 4 cut(s) 232, 741, 868, 884
AvaII GGWCC 1 cut(s) 30
AxyI CCTNAGG 1 cut(s) 170
BaeGI GKGCMC 1 cut(s) 843
BanI GGYRCC 2 cut(s) 187, 965
BanII GRGCYC 1 cut(s) 778
BauI CACGAG 1 cut(s) 777
Bbv12I GWGCWC 1 cut(s) 778
BbvI GCAGC 3 cut(s) 290, 578, 931
BccI CCATC 3 cut(s) 69, 110, 347
BciT130I CCWGG 4 cut(s) 247, 314, 529, 970
BciVI GTATCC 1 cut(s) 1020
BcnI CCSGG 1 cut(s) 28
BcoDI GTCTC 2 cut(s) 291, 751
BfaI CTAG 1 cut(s) 771
BfmI CTRYAG 1 cut(s) 1021
BfuAI ACCTGC 1 cut(s) 590
BfuI GTATCC 1 cut(s) 1020
BisI GCNGC 4 cut(s) 11, 304, 567, 945
BlsI GCNGC 4 cut(s) 12, 305, 568, 946
Bme1390I CCNGG 5 cut(s) 28, 247, 314, 529, 970
Bme18I GGWCC 1 cut(s) 30
BmgT120I GGNCC 2 cut(s) 30, 310
BmiI GGNNCC 2 cut(s) 189, 967
BmrFI CCNGG 5 cut(s) 28, 247, 314, 529, 970
BmsI GCATC 2 cut(s) 457, 793
BplI GAGNNNNNCTC 2 cut(s) 123, 155
BpuEI CTTGAG 2 cut(s) 125, 531
BpuMI CCSGG 1 cut(s) 28
Bsa29I ATCGAT 1 cut(s) 1060
BsaBI GATNNNNATC 2 cut(s) 332, 402
BsaHI GRCGYC 2 cut(s) 5, 832
BsaI GGTCTC 1 cut(s) 751
BsaJI CCNNGG 2 cut(s) 528, 969
BsaXI ACNNNNNCTCC 2 cut(s) 409, 439
Bsc4I CCNNNNNNNGG 3 cut(s) 518, 758, 759
Bse21I CCTNAGG 1 cut(s) 170
Bse8I GATNNNNATC 2 cut(s) 332, 402
BseBI CCWGG 4 cut(s) 247, 314, 529, 970
BseCI ATCGAT 1 cut(s) 1060
BseDI CCNNGG 2 cut(s) 528, 969
BseGI GGATG 2 cut(s) 102, 910
BseJI GATNNNNATC 2 cut(s) 332, 402
BseLI CCNNNNNNNGG 3 cut(s) 518, 758, 759
BseMII CTCAG 3 cut(s) 184, 265, 302
BseRI GAGGAG 1 cut(s) 158
BseSI GKGCMC 1 cut(s) 843
BseXI GCAGC 3 cut(s) 290, 578, 931
BseYI CCCAGC 1 cut(s) 923
Bsh1236I CGCG 2 cut(s) 48, 788
BshFI GGCC 1 cut(s) 312
BshNI GGYRCC 2 cut(s) 187, 965
BshVI ATCGAT 1 cut(s) 1060
BsiHKAI GWGCWC 1 cut(s) 778
BsiSI CCGG 2 cut(s) 28, 264
BslFI GGGAC 2 cut(s) 10, 940
BslI CCNNNNNNNGG 3 cut(s) 518, 758, 759
BsmAI GTCTC 2 cut(s) 291, 751
BsmFI GGGAC 2 cut(s) 10, 940
BsnI GGCC 1 cut(s) 312
Bso31I GGTCTC 1 cut(s) 751
Bsp1286I GDGCHC 2 cut(s) 778, 843
Bsp1407I TGTACA 1 cut(s) 989
Bsp143I GATC 4 cut(s) 333, 625, 670, 1057
Bsp68I TCGCGA 2 cut(s) 48, 788
BspACI CCGC 2 cut(s) 851, 998
BspANI GGCC 1 cut(s) 312
BspCNI CTCAG 3 cut(s) 183, 264, 301
BspDI ATCGAT 1 cut(s) 1060
BspFNI CGCG 2 cut(s) 48, 788
BspHI TCATGA 1 cut(s) 459
BspLI GGNNCC 2 cut(s) 189, 967
BspMI ACCTGC 1 cut(s) 590
BspPI GGATC 1 cut(s) 678
BspT107I GGYRCC 2 cut(s) 187, 965
BspTNI GGTCTC 1 cut(s) 751
BsrGI TGTACA 1 cut(s) 989
BssECI CCNNGG 2 cut(s) 528, 969
BssMI GATC 4 cut(s) 333, 625, 670, 1057
BssNI GRCGYC 2 cut(s) 5, 832
BssSI CACGAG 1 cut(s) 777
Bst2BI CACGAG 1 cut(s) 777
Bst2UI CCWGG 4 cut(s) 247, 314, 529, 970
BstACI GRCGYC 2 cut(s) 5, 832
BstAUI TGTACA 1 cut(s) 989
BstC8I GCNNGC 2 cut(s) 301, 712
BstDEI CTNAG 4 cut(s) 170, 251, 288, 341
BstF5I GGATG 2 cut(s) 102, 910
BstFNI CGCG 2 cut(s) 48, 788
BstHHI GCGC 1 cut(s) 283
BstKTI GATC 4 cut(s) 336, 628, 673, 1060
BstMAI GTCTC 2 cut(s) 291, 751
BstMBI GATC 4 cut(s) 333, 625, 670, 1057
BstMWI GCNNNNNNNGC 3 cut(s) 278, 309, 812
BstNI CCWGG 4 cut(s) 247, 314, 529, 970
BstNSI RCATGY 4 cut(s) 423, 624, 987, 998
BstSCI CCNGG 5 cut(s) 26, 245, 312, 527, 968
BstSFI CTRYAG 1 cut(s) 1021
BstSLI GKGCMC 1 cut(s) 843
BstUI CGCG 2 cut(s) 48, 788
BstV1I GCAGC 3 cut(s) 290, 578, 931
Bsu15I ATCGAT 1 cut(s) 1060
Bsu36I CCTNAGG 1 cut(s) 170
BsuI GTATCC 1 cut(s) 1020
BsuRI GGCC 1 cut(s) 312
BsuTUI ATCGAT 1 cut(s) 1060
BtgZI GCGATG 1 cut(s) 47
BtsCI GGATG 2 cut(s) 102, 910
BtsI GCAGTG 2 cut(s) 267, 498
BtsIMutI CAGTG 2 cut(s) 267, 498
BtuMI TCGCGA 2 cut(s) 48, 788
BveI ACCTGC 1 cut(s) 590
Cac8I GCNNGC 2 cut(s) 301, 712
CciI TCATGA 1 cut(s) 459
CfoI GCGC 1 cut(s) 283
Cfr13I GGNCC 2 cut(s) 30, 310
ClaI ATCGAT 1 cut(s) 1060
CseI GACGC 1 cut(s) 12
Csp6I GTAC 2 cut(s) 145, 990
CviAII CATG 8 cut(s) 307, 420, 460, 621, 674, 910, 984, 995
CviQI GTAC 2 cut(s) 145, 990
DdeI CTNAG 4 cut(s) 170, 251, 288, 341
DpnI GATC 4 cut(s) 335, 627, 672, 1059
DpnII GATC 4 cut(s) 333, 625, 670, 1057
DraIII CACNNNGTG 1 cut(s) 847
EciI GGCGGA 1 cut(s) 866
Ecl136II GAGCTC 1 cut(s) 776
Eco24I GRGCYC 1 cut(s) 778
Eco31I GGTCTC 1 cut(s) 751
Eco47I GGWCC 1 cut(s) 30
Eco53kI GAGCTC 1 cut(s) 776
Eco57I CTGAAG 1 cut(s) 468
Eco81I CCTNAGG 1 cut(s) 170
EcoICRI GAGCTC 1 cut(s) 776
EcoRI GAATTC 1 cut(s) 109
EcoRII CCWGG 4 cut(s) 245, 312, 527, 968
EcoT22I ATGCAT 1 cut(s) 985
EcoT38I GRGCYC 1 cut(s) 778
FaeI CATG 8 cut(s) 310, 423, 463, 624, 677, 913, 987, 998
FaqI GGGAC 2 cut(s) 10, 940
FatI CATG 8 cut(s) 306, 419, 459, 620, 673, 909, 983, 994
Fnu4HI GCNGC 4 cut(s) 11, 304, 567, 945
FokI GGATG 2 cut(s) 89, 917
FriOI GRGCYC 1 cut(s) 778
Fsp4HI GCNGC 4 cut(s) 11, 304, 567, 945
FspBI CTAG 1 cut(s) 771
GlaI GCGC 1 cut(s) 282
GluI GCNGC 4 cut(s) 11, 304, 567, 945
GsaI CCCAGC 1 cut(s) 927
HaeIII GGCC 1 cut(s) 312
HapII CCGG 2 cut(s) 28, 264
HgaI GACGC 1 cut(s) 12
HhaI GCGC 1 cut(s) 283
Hin1I GRCGYC 2 cut(s) 5, 832
Hin1II CATG 8 cut(s) 310, 423, 463, 624, 677, 913, 987, 998
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HinfI GANTC 5 cut(s) 165, 255, 508, 544, 557
HpaII CCGG 2 cut(s) 28, 264
HphI GGTGA 4 cut(s) 232, 741, 868, 884
Hpy166II GTNNAC 1 cut(s) 992
Hpy188I TCNGA 5 cut(s) 202, 254, 477, 730, 802
Hpy188III TCNNGA 7 cut(s) 47, 106, 172, 290, 460, 787, 1050
Hpy8I GTNNAC 1 cut(s) 992
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 1 cut(s) 318
HpyCH4IV ACGT 1 cut(s) 832
HpyCH4V TGCA 9 cut(s) 306, 376, 412, 431, 566, 581, 605, 620, 983
HpyF10VI GCNNNNNNNGC 3 cut(s) 278, 309, 812
HpyF3I CTNAG 4 cut(s) 170, 251, 288, 341
HpySE526I ACGT 1 cut(s) 832
Hsp92I GRCGYC 2 cut(s) 5, 832
Hsp92II CATG 8 cut(s) 310, 423, 463, 624, 677, 913, 987, 998
HspAI GCGC 1 cut(s) 281
Kzo9I GATC 4 cut(s) 333, 625, 670, 1057
LmnI GCTCC 2 cut(s) 448, 811
Lsp1109I GCAGC 3 cut(s) 290, 578, 931
LweI GCATC 2 cut(s) 457, 793
MaeI CTAG 1 cut(s) 771
MaeII ACGT 1 cut(s) 832
MaeIII GTNAC 1 cut(s) 833
MalI GATC 4 cut(s) 335, 627, 672, 1059
MboI GATC 4 cut(s) 333, 625, 670, 1057
MboII GAAGA 2 cut(s) 191, 620
MhlI GDGCHC 2 cut(s) 778, 843
MluCI AATT 7 cut(s) 109, 193, 228, 294, 583, 794, 862
MlyI GAGTC 2 cut(s) 159, 538
MmeI TCCRAC 1 cut(s) 455
Mph1103I ATGCAT 1 cut(s) 985
MroXI GAANNNNTTC 1 cut(s) 259
MseI TTAA 1 cut(s) 330
MslI CAYNNNNRTG 2 cut(s) 503, 678
MspA1I CMGCKG 1 cut(s) 923
MspI CCGG 2 cut(s) 28, 264
MspR9I CCNGG 5 cut(s) 28, 247, 314, 529, 970
MvaI CCWGG 4 cut(s) 247, 314, 529, 970
MvnI CGCG 2 cut(s) 48, 788
MwoI GCNNNNNNNGC 3 cut(s) 278, 309, 812
NciI CCSGG 1 cut(s) 28
NdeII GATC 4 cut(s) 333, 625, 670, 1057
NlaIII CATG 8 cut(s) 310, 423, 463, 624, 677, 913, 987, 998
NlaIV GGNNCC 2 cut(s) 189, 967
NmuCI GTSAC 1 cut(s) 833
NruI TCGCGA 2 cut(s) 48, 788
NsiI ATGCAT 1 cut(s) 985
NspI RCATGY 4 cut(s) 423, 624, 987, 998
OliI CACNNNNGTG 1 cut(s) 503
PagI TCATGA 1 cut(s) 459
PaqCI CACCTGC 1 cut(s) 590
PciI ACATGT 1 cut(s) 419
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PdmI GAANNNNTTC 1 cut(s) 259
PfeI GAWTC 3 cut(s) 255, 508, 557
PflMI CCANNNNNTGG 1 cut(s) 518
PkrI GCNGC 4 cut(s) 12, 305, 568, 946
PleI GAGTC 2 cut(s) 159, 538
PpsI GAGTC 2 cut(s) 159, 538
PscI ACATGT 1 cut(s) 419
Psp124BI GAGCTC 1 cut(s) 778
Psp6I CCWGG 4 cut(s) 245, 312, 527, 968
PspFI CCCAGC 1 cut(s) 923
PspGI CCWGG 4 cut(s) 245, 312, 527, 968
PspN4I GGNNCC 2 cut(s) 189, 967
PspPI GGNCC 2 cut(s) 30, 310
PvuII CAGCTG 1 cut(s) 923
RruI TCGCGA 2 cut(s) 48, 788
RsaI GTAC 2 cut(s) 146, 991
RsaNI GTAC 2 cut(s) 145, 990
RseI CAYNNNNRTG 2 cut(s) 503, 678
SacI GAGCTC 1 cut(s) 778
SaqAI TTAA 1 cut(s) 330
SatI GCNGC 4 cut(s) 11, 304, 567, 945
Sau3AI GATC 4 cut(s) 333, 625, 670, 1057
Sau96I GGNCC 2 cut(s) 30, 310
SchI GAGTC 2 cut(s) 159, 538
ScrFI CCNGG 5 cut(s) 28, 247, 314, 529, 970
SduI GDGCHC 2 cut(s) 778, 843
SfaNI GCATC 2 cut(s) 457, 793
SfcI CTRYAG 1 cut(s) 1021
SinI GGWCC 1 cut(s) 30
SmiMI CAYNNNNRTG 2 cut(s) 503, 678
SmlI CTYRAG 2 cut(s) 140, 546
SmoI CTYRAG 2 cut(s) 140, 546
Sse9I AATT 7 cut(s) 109, 193, 228, 294, 583, 794, 862
SsiI CCGC 2 cut(s) 851, 998
SspMI CTAG 1 cut(s) 771
SstI GAGCTC 1 cut(s) 778
StyD4I CCNGG 5 cut(s) 26, 245, 312, 527, 968
TaiI ACGT 1 cut(s) 835
TaqI TCGA 3 cut(s) 336, 1035, 1060
TasI AATT 7 cut(s) 109, 193, 228, 294, 583, 794, 862
TatI WGTACW 1 cut(s) 989
TfiI GAWTC 3 cut(s) 255, 508, 557
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TscAI CASTG 2 cut(s) 274, 505
TseFI GTSAC 1 cut(s) 833
TseI GCWGC 4 cut(s) 10, 303, 566, 944
Tsp45I GTSAC 1 cut(s) 833
TspDTI ATGAA 5 cut(s) 240, 450, 620, 875, 950
TspGWI ACGGA 2 cut(s) 103, 177
TspRI CASTG 2 cut(s) 274, 505
Van91I CCANNNNNTGG 1 cut(s) 518
VpaK11BI GGWCC 1 cut(s) 30
XapI RAATTY 4 cut(s) 109, 193, 583, 862
XceI RCATGY 4 cut(s) 423, 624, 987, 998
XmnI GAANNNNTTC 1 cut(s) 259
XspI CTAG 1 cut(s) 771
ZraI GACGTC 1 cut(s) 833
Zsp2I ATGCAT 1 cut(s) 985
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.