pycom07g12810
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0008521
Physical Location & Seq
Reverse (-)
14137588 .. 14139108
1521 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 666 bp
ATGGAGGATCATCATCAACAGCATAATCCACCTCCATTAACCTCCCAATTGCCGCCCTTATCGTTACCATTCTTGTTGCCCCCGCCACCCCAAAACCCTCCTCTTCCTTCTCCCTATCTCTTCACACCCTCTTCATCCTCCTCACTGATGACCAACCCACCTGATCTTCTAGACATTGATTGGGTCAGCCTTCTCTCTGGTGGTACTCAGGCGGCTGGCTGGCTTAGTCATGATCAGATTAATAGTTTTAAGCCTATGGCTATGGTGGACAAAAATATTAGTGGCTCTGATGGGACTATTATTAGTGAAGCTGCAGATCAGGAAGAAAAGGTGGGTATTAAAAGGAAAGCTGGGGGTAGGATGAGAAAAGCAGCGGCAAGCCGACCTAGGTTTGCGTTCCATACTCGGAGTACGGATGATATTCTTGATGATGGCTACCGCTGGAGAAAATATGGACAGAAAGCCGTCAAGAATAGCTCATATCCCAGGAGCTATTATCGCTGCACACATCACACATGCAATGTGAAAAAACAGGTCCAGAGGCTGTCTAAAGACACGAGCATCGTCGTGACAACGTATGAAGGAATTCACAATCATCCTTGCGAGAAACTCATGGAAACCCTAACTCCTCTACTGAAGCAAATGCAGTTTCTCTCTAGGTTTTAA

Protein Analysis

222

Amino Acids

24.88

Weight (kDa)

9.24

Isoelectric Point (pI)

43.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 143 - 200 1.2e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 53, 83, 212, 374, 439
AclWI GGATC 1 cut(s) 15
AcsI RAATTY 1 cut(s) 585
AcuI CTGAAG 1 cut(s) 656
AfaI GTAC 2 cut(s) 205, 412
AjnI CCWGG 1 cut(s) 485
AluBI AGCT 4 cut(s) 311, 350, 477, 492
AluI AGCT 4 cut(s) 311, 350, 477, 492
AlwI GGATC 1 cut(s) 15
AlwNI CAGNNNCTG 1 cut(s) 544
ApeKI GCWGC 3 cut(s) 311, 371, 501
ApoI RAATTY 1 cut(s) 585
AseI ATTAAT 1 cut(s) 240
Asp700I GAANNNNTTC 1 cut(s) 585
AspA2I CCTAGG 1 cut(s) 386
AspS9I GGNCC 1 cut(s) 535
AvaII GGWCC 1 cut(s) 535
AvrII CCTAGG 1 cut(s) 386
BauI CACGAG 1 cut(s) 556
BbvI GCAGC 3 cut(s) 298, 383, 488
BccI CCATC 2 cut(s) 284, 425
BceAI ACGGC 1 cut(s) 449
BciT130I CCWGG 1 cut(s) 487
BclI TGATCA 1 cut(s) 232
BfaI CTAG 3 cut(s) 170, 387, 657
BfmI CTRYAG 1 cut(s) 312
BisI GCNGC 6 cut(s) 53, 213, 312, 372, 375, 502
BlnI CCTAGG 1 cut(s) 386
BlsI GCNGC 6 cut(s) 54, 214, 313, 373, 376, 503
Bme1390I CCNGG 1 cut(s) 487
Bme18I GGWCC 1 cut(s) 535
BmgT120I GGNCC 1 cut(s) 535
BmrFI CCNGG 1 cut(s) 487
BmsI GCATC 1 cut(s) 570
BpmI CTGGAG 1 cut(s) 463
BsaBI GATNNNNATC 1 cut(s) 12
BsaJI CCNNGG 2 cut(s) 386, 485
BsaXI ACNNNNNCTCC 2 cut(s) 610, 640
Bse3DI GCAATG 1 cut(s) 526
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 1 cut(s) 487
BseDI CCNNGG 2 cut(s) 386, 485
BseGI GGATG 4 cut(s) 134, 366, 421, 595
BseJI GATNNNNATC 1 cut(s) 12
BseMI GCAATG 1 cut(s) 526
BseMII CTCAG 1 cut(s) 221
BseRI GAGGAG 3 cut(s) 90, 130, 618
BseXI GCAGC 3 cut(s) 298, 383, 488
BseYI CCCAGC 1 cut(s) 350
BsgI GTGCAG 1 cut(s) 487
BslFI GGGAC 1 cut(s) 307
BsmFI GGGAC 1 cut(s) 307
Bsp143I GATC 4 cut(s) 7, 163, 232, 316
BspACI CCGC 5 cut(s) 53, 83, 212, 374, 439
BspCNI CTCAG 1 cut(s) 220
BspHI TCATGA 1 cut(s) 229
BspMAI CTGCAG 1 cut(s) 316
BspPI GGATC 1 cut(s) 15
BsrDI GCAATG 1 cut(s) 526
BssECI CCNNGG 2 cut(s) 386, 485
BssMI GATC 4 cut(s) 7, 163, 232, 316
BssSI CACGAG 1 cut(s) 556
BssT1I CCWWGG 1 cut(s) 386
Bst2BI CACGAG 1 cut(s) 556
Bst2UI CCWGG 1 cut(s) 487
Bst6I CTCTTC 3 cut(s) 108, 125, 136
BstC8I GCNNGC 3 cut(s) 217, 221, 379
BstDEI CTNAG 2 cut(s) 207, 224
BstF5I GGATG 4 cut(s) 134, 366, 421, 595
BstKTI GATC 4 cut(s) 10, 166, 235, 319
BstMBI GATC 4 cut(s) 7, 163, 232, 316
BstMWI GCNNNNNNNGC 1 cut(s) 498
BstNI CCWGG 1 cut(s) 487
BstNSI RCATGY 1 cut(s) 519
BstSCI CCNGG 1 cut(s) 485
BstSFI CTRYAG 1 cut(s) 312
BstV1I GCAGC 3 cut(s) 298, 383, 488
BtsCI GGATG 4 cut(s) 134, 366, 421, 595
BtsIMutI CAGTG 1 cut(s) 143
Cac8I GCNNGC 3 cut(s) 217, 221, 379
CaiI CAGNNNCTG 1 cut(s) 544
CciI TCATGA 1 cut(s) 229
Cfr13I GGNCC 1 cut(s) 535
Csp6I GTAC 2 cut(s) 204, 411
CviAII CATG 3 cut(s) 230, 516, 613
CviQI GTAC 2 cut(s) 204, 411
DdeI CTNAG 2 cut(s) 207, 224
DpnI GATC 4 cut(s) 9, 165, 234, 318
DpnII GATC 4 cut(s) 7, 163, 232, 316
Eam1104I CTCTTC 3 cut(s) 108, 125, 136
EarI CTCTTC 3 cut(s) 108, 125, 136
Eco130I CCWWGG 1 cut(s) 386
Eco47I GGWCC 1 cut(s) 535
Eco57I CTGAAG 1 cut(s) 656
EcoRI GAATTC 1 cut(s) 585
EcoRII CCWGG 1 cut(s) 485
EcoT14I CCWWGG 1 cut(s) 386
ErhI CCWWGG 1 cut(s) 386
FaeI CATG 3 cut(s) 233, 519, 616
FaqI GGGAC 1 cut(s) 307
FatI CATG 3 cut(s) 229, 515, 612
FauI CCCGC 1 cut(s) 90
FbaI TGATCA 1 cut(s) 232
Fnu4HI GCNGC 6 cut(s) 53, 213, 312, 372, 375, 502
FokI GGATG 4 cut(s) 121, 373, 428, 582
Fsp4HI GCNGC 6 cut(s) 53, 213, 312, 372, 375, 502
FspBI CTAG 3 cut(s) 170, 387, 657
GluI GCNGC 6 cut(s) 53, 213, 312, 372, 375, 502
GsaI CCCAGC 1 cut(s) 354
GsuI CTGGAG 1 cut(s) 463
Hin1II CATG 3 cut(s) 233, 519, 616
Hpy166II GTNNAC 1 cut(s) 268
Hpy188I TCNGA 3 cut(s) 237, 289, 408
Hpy188III TCNNGA 7 cut(s) 170, 230, 320, 425, 469, 538, 568
Hpy8I GTNNAC 1 cut(s) 268
Hpy99I CGWCG 1 cut(s) 569
HpyAV CCTTC 3 cut(s) 117, 200, 575
HpyCH4IV ACGT 1 cut(s) 575
HpyCH4V TGCA 4 cut(s) 314, 504, 519, 646
HpyF10VI GCNNNNNNNGC 1 cut(s) 498
HpyF3I CTNAG 2 cut(s) 207, 224
HpySE526I ACGT 1 cut(s) 575
Hsp92II CATG 3 cut(s) 233, 519, 616
Ksp22I TGATCA 1 cut(s) 232
Kzo9I GATC 4 cut(s) 7, 163, 232, 316
LmnI GCTCC 1 cut(s) 489
Lsp1109I GCAGC 3 cut(s) 298, 383, 488
LweI GCATC 1 cut(s) 570
MaeI CTAG 3 cut(s) 170, 387, 657
MaeII ACGT 1 cut(s) 575
MaeIII GTNAC 2 cut(s) 63, 568
MalI GATC 4 cut(s) 9, 165, 234, 318
MboI GATC 4 cut(s) 7, 163, 232, 316
MboII GAAGA 5 cut(s) 95, 112, 123, 158, 335
MfeI CAATTG 1 cut(s) 47
MluCI AATT 2 cut(s) 47, 585
MnlI CCTC 9 cut(s) 42, 52, 108, 111, 139, 148, 151, 534, 639
MroXI GAANNNNTTC 1 cut(s) 585
MseI TTAA 5 cut(s) 38, 240, 249, 339, 664
MslI CAYNNNNRTG 1 cut(s) 566
MspA1I CMGCKG 2 cut(s) 374, 441
MspR9I CCNGG 1 cut(s) 487
MunI CAATTG 1 cut(s) 47
MvaI CCWGG 1 cut(s) 487
MwoI GCNNNNNNNGC 1 cut(s) 498
NdeII GATC 4 cut(s) 7, 163, 232, 316
NlaIII CATG 3 cut(s) 233, 519, 616
NmuCI GTSAC 1 cut(s) 568
NspI RCATGY 1 cut(s) 519
PagI TCATGA 1 cut(s) 229
PdmI GAANNNNTTC 1 cut(s) 585
PkrI GCNGC 6 cut(s) 54, 214, 313, 373, 376, 503
PshBI ATTAAT 1 cut(s) 240
Psp6I CCWGG 1 cut(s) 485
PspFI CCCAGC 1 cut(s) 350
PspGI CCWGG 1 cut(s) 485
PspPI GGNCC 1 cut(s) 535
PstI CTGCAG 1 cut(s) 316
PstNI CAGNNNCTG 1 cut(s) 544
RsaI GTAC 2 cut(s) 205, 412
RsaNI GTAC 2 cut(s) 204, 411
RseI CAYNNNNRTG 1 cut(s) 566
SaqAI TTAA 5 cut(s) 38, 240, 249, 339, 664
SatI GCNGC 6 cut(s) 53, 213, 312, 372, 375, 502
Sau3AI GATC 4 cut(s) 7, 163, 232, 316
Sau96I GGNCC 1 cut(s) 535
ScrFI CCNGG 1 cut(s) 487
SfaNI GCATC 1 cut(s) 570
SfcI CTRYAG 1 cut(s) 312
SinI GGWCC 1 cut(s) 535
SmiMI CAYNNNNRTG 1 cut(s) 566
Sse9I AATT 2 cut(s) 47, 585
SsiI CCGC 5 cut(s) 53, 83, 212, 374, 439
SspI AATATT 1 cut(s) 277
SspMI CTAG 3 cut(s) 170, 387, 657
StyD4I CCNGG 1 cut(s) 485
StyI CCWWGG 1 cut(s) 386
TaiI ACGT 1 cut(s) 578
TasI AATT 2 cut(s) 47, 585
TauI GCSGC 3 cut(s) 55, 215, 377
Tru1I TTAA 5 cut(s) 38, 240, 249, 339, 664
Tru9I TTAA 5 cut(s) 38, 240, 249, 339, 664
TscAI CASTG 1 cut(s) 150
TseFI GTSAC 1 cut(s) 568
TseI GCWGC 3 cut(s) 311, 371, 501
Tsp45I GTSAC 1 cut(s) 568
TspDTI ATGAA 2 cut(s) 123, 594
TspGWI ACGGA 1 cut(s) 428
TspRI CASTG 1 cut(s) 150
VpaK11BI GGWCC 1 cut(s) 535
VspI ATTAAT 1 cut(s) 240
XapI RAATTY 1 cut(s) 585
XbaI TCTAGA 1 cut(s) 169
XceI RCATGY 1 cut(s) 519
XmaJI CCTAGG 1 cut(s) 386
XmnI GAANNNNTTC 1 cut(s) 585
XspI CTAG 3 cut(s) 170, 387, 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.