pycom07g15930

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0013430
Physical Location & Seq
Reverse (-)
18409954 .. 18423188
13235 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1416 bp
ATGGATACTGAAAATTTTGAGACGTTGCAGGGTTTAGGTGCGGGTTCTAATGCGGCAAACATTAATCGCAGAGAAAGTGAGAGCCTTTCAAGGAGCTACAGCGGCAGCTGCATTTCGTTGCCGTCGATAGCAGAGCCAAAGCCAGAGACTTTGGCAGAGCCTTGCTTAGTCTCGTGCACGACTTTCAATATTTTGGCTCCAATCTACAAAAGACTCGATCAACAGAATCAAAGCCTTAGAGAGAGCGACTACAGAGCGTTTTGGGTGGCCAGGAACCAGAGGATTTTGGATTGGTTGCTCTGTGAATCATCTGCCATTATTTGTCTGCAGGAGTTCTGGGTTGGAAATGAAGAATTTGTGAATATGTACCTGGACTGGCTTGGTGATGCAGGCTATACTACCTTTAAGCTCGCCCGAACCAACAACCGGGGAGATGGTTTGCTGACTGCTGTGAGGAGGGACTGCTTTAGTGTGCGAAACTACCGAGAGTTGCATTTTAATGATTTTGGAGACCGTGTTGCTCAGCTATTACATGTTCAGTTAGCTGCCCCGTTTTCACAAAACCAGAGAGGAAACGTTCAACAAGAAATGCTAATTGTGAATACTCATTTGTTGTTTCCTCATGATTCCAGTCTCTCTATAGTACGATTGCATCAGGTTTACAAAATTTTGCAATTTGTGGAGTCATATCAGAATGAAAAGAAGCTAAACCCGATGCCTATCGTACTCTGTGGTGACTGGAATGGAAGTAAACAAGGCCATGTGTACAAATTCCTTAGATCACAGGGTTTTGAATCAACATATGACACTGCTCATCAATATACCGATGCGGATGCTCACAAGTGGGTGAGCCACCGCAATCATAGGGGAAACATCTGCGGTGTTGACTTCATTTGGCTTCGTAATCCCAATAAGTCACGAAAACCACTAAAGACTAGTTGGTGCGAAGCTGTTTTTGGAATATTACGGCGTCAGCTTCAAAAAGCTTCGATGGCTGAAAATGATGCATTTGCCTTTCTGAAGGGTGATAGTCATGGTGATTTTATTACGTCCTCAGCTTTCTGTGAAGCACTACAACAGGTTAATTTAATTGATCATCCTACGGGACTAGGTTTCCAGGAGACAAGGGATCTCTGGATCCAGGCAGATGCCGACGCAAATGGTGTTCTTGATTACGAAGAATTTAAGAATAGAATTTGGAGTTCGACAGCGTCAGAGGATAAAGAAAGTCTCAACGGTAGTAGAGAGGAGTCCAAACGAGGCACGCAGGACGCCCTCGGATTCAACGTGAAGAAGGCAGTTCTATACCCCCCTGAGGCGGAGAAGGGAATATGGCCCGAAGATTATACTCTCTCTGATCACGCTCCACTCTCGGTTGTACTTTCACCAGAAAGGATGTGGTGTTGTAAGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8 Exo_endo_phos

Protein Analysis

472

Amino Acids

53.69

Weight (kDa)

5.85

Isoelectric Point (pI)

44.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 61 - 316 1.8e-08 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013430)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02270 AT1G02270
fragaria_vesca FvH4_7g18080 FvH4_7g18080 FvH4_7g18080
malus_domestica MD01G1095200.v1.1 MD07G1163500.v1.1 MD07G1164200.v1.1
prunus_persica Prupe.2G203200_v2.0.a1
pyrus_communis pycom01g11740 pycom07g15930
rosa_chinensis RchiOBHm_Chr1g0361711
rosa_laevigata RLG00000027708
rosa_multiflora Rmu_sc0003426.1_g000004
rosa_rugosa Rorug01G0291000
rosa_samantha Rh1BG264200 Rh1DG294200
rosa_wichuraiana Rw1G026710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 7 cut(s) 41, 53, 102, 830, 856, 879, 1319
AclI AACGTT 1 cut(s) 576
AclWI GGATC 3 cut(s) 1132, 1137, 1145
AcoI YGGCCR 1 cut(s) 267
AcsI RAATTY 6 cut(s) 13, 353, 666, 770, 1181, 1194
AcuI CTGAAG 1 cut(s) 1040
AcyI GRCGYC 2 cut(s) 970, 1272
AfaI GTAC 5 cut(s) 368, 645, 726, 767, 1380
AfiI CCNNNNNNNGG 3 cut(s) 426, 1315, 1318
AflIII ACRYGT 1 cut(s) 532
AgsI TTSAA 6 cut(s) 90, 187, 581, 794, 980, 1285
AhlI ACTAGT 1 cut(s) 935
AjnI CCWGG 4 cut(s) 269, 369, 1116, 1140
AjuI GAANNNNNNNTTGG 2 cut(s) 939, 971
Alw21I GWGCWC 1 cut(s) 179
Alw26I GTCTC 7 cut(s) 14, 140, 175, 504, 638, 1115, 1235
Alw44I GTGCAC 1 cut(s) 175
AlwI GGATC 3 cut(s) 1132, 1137, 1145
AoxI GGCC 3 cut(s) 267, 757, 1334
ApaLI GTGCAC 1 cut(s) 175
ApeKI GCWGC 3 cut(s) 105, 108, 545
ApoI RAATTY 6 cut(s) 13, 353, 666, 770, 1181, 1194
AseI ATTAAT 1 cut(s) 63
AspS9I GGNCC 1 cut(s) 1335
AsuC2I CCSGG 1 cut(s) 428
AsuHPI GGTGA 6 cut(s) 395, 746, 859, 1037, 1049, 1377
AxyI CCTNAGG 1 cut(s) 1314
BaeGI GKGCMC 1 cut(s) 179
BalI TGGCCA 1 cut(s) 269
BamHI GGATCC 1 cut(s) 1137
BauI CACGAG 1 cut(s) 172
Bbv12I GWGCWC 1 cut(s) 179
BbvCI CCTCAGC 1 cut(s) 1054
BbvI GCAGC 3 cut(s) 95, 117, 532
BccI CCATC 2 cut(s) 428, 985
BceAI ACGGC 2 cut(s) 106, 983
BcgI CGANNNNNNTGC 2 cut(s) 815, 849
BciT130I CCWGG 4 cut(s) 271, 371, 1118, 1142
BclI TGATCA 2 cut(s) 1093, 1357
BcnI CCSGG 1 cut(s) 428
BcoDI GTCTC 7 cut(s) 14, 140, 175, 504, 638, 1115, 1235
BcuI ACTAGT 1 cut(s) 935
BfaI CTAG 2 cut(s) 936, 1109
BfmI CTRYAG 4 cut(s) 97, 250, 326, 639
BisI GCNGC 5 cut(s) 54, 103, 106, 109, 546
BlpI GCTNAGC 1 cut(s) 522
BlsI GCNGC 5 cut(s) 55, 104, 107, 110, 547
Bme1390I CCNGG 5 cut(s) 271, 371, 428, 1118, 1142
BmgT120I GGNCC 1 cut(s) 1335
BmiI GGNNCC 3 cut(s) 198, 275, 1139
BmrFI CCNGG 5 cut(s) 271, 371, 428, 1118, 1142
BmsI GCATC 7 cut(s) 376, 661, 705, 817, 823, 994, 1138
Bpu10I CCTNAGC 1 cut(s) 1054
Bpu1102I GCTNAGC 1 cut(s) 522
BpuMI CCSGG 1 cut(s) 428
BsaBI GATNNNNATC 1 cut(s) 719
BsaHI GRCGYC 2 cut(s) 970, 1272
BsaI GGTCTC 1 cut(s) 504
BsaJI CCNNGG 2 cut(s) 427, 1276
BsaXI ACNNNNNCTCC 2 cut(s) 501, 531
Bsc4I CCNNNNNNNGG 3 cut(s) 426, 1315, 1318
Bse1I ACTGG 3 cut(s) 380, 630, 743
Bse21I CCTNAGG 1 cut(s) 1314
Bse8I GATNNNNATC 1 cut(s) 719
BseBI CCWGG 4 cut(s) 271, 371, 1118, 1142
BseDI CCNNGG 2 cut(s) 427, 1276
BseGI GGATG 3 cut(s) 838, 1096, 1401
BseJI GATNNNNATC 1 cut(s) 719
BseLI CCNNNNNNNGG 3 cut(s) 426, 1315, 1318
BseMII CTCAG 3 cut(s) 536, 1068, 1305
BseNI ACTGG 3 cut(s) 380, 630, 743
BseRI GAGGAG 2 cut(s) 469, 1262
BseSI GKGCMC 1 cut(s) 179
BseXI GCAGC 3 cut(s) 95, 117, 532
BshFI GGCC 3 cut(s) 269, 759, 1336
BsiHKAI GWGCWC 1 cut(s) 179
BsiSI CCGG 1 cut(s) 427
BslFI GGGAC 2 cut(s) 473, 1119
BslI CCNNNNNNNGG 3 cut(s) 426, 1315, 1318
BsmAI GTCTC 7 cut(s) 14, 140, 175, 504, 638, 1115, 1235
BsmBI CGTCTC 1 cut(s) 14
BsmFI GGGAC 2 cut(s) 473, 1119
BsnI GGCC 3 cut(s) 269, 759, 1336
Bso31I GGTCTC 1 cut(s) 504
Bsp1286I GDGCHC 1 cut(s) 179
Bsp1407I TGTACA 1 cut(s) 765
Bsp143I GATC 6 cut(s) 217, 779, 1093, 1129, 1137, 1357
Bsp1720I GCTNAGC 1 cut(s) 522
BspACI CCGC 7 cut(s) 41, 53, 102, 830, 856, 879, 1319
BspANI GGCC 3 cut(s) 269, 759, 1336
BspCNI CTCAG 3 cut(s) 535, 1067, 1306
BspHI TCATGA 1 cut(s) 622
BspLI GGNNCC 3 cut(s) 198, 275, 1139
BspMAI CTGCAG 1 cut(s) 330
BspPI GGATC 3 cut(s) 1132, 1137, 1145
BspTNI GGTCTC 1 cut(s) 504
BsrGI TGTACA 1 cut(s) 765
BsrI ACTGG 3 cut(s) 380, 630, 743
BssECI CCNNGG 2 cut(s) 427, 1276
BssMI GATC 6 cut(s) 217, 779, 1093, 1129, 1137, 1357
BssNI GRCGYC 2 cut(s) 970, 1272
BssSI CACGAG 1 cut(s) 172
Bst2BI CACGAG 1 cut(s) 172
Bst2UI CCWGG 4 cut(s) 271, 371, 1118, 1142
Bst4CI ACNGT 2 cut(s) 515, 1238
BstACI GRCGYC 2 cut(s) 970, 1272
BstAUI TGTACA 1 cut(s) 765
BstC8I GCNNGC 3 cut(s) 391, 411, 1265
BstDEI CTNAG 6 cut(s) 166, 236, 522, 776, 1054, 1314
BstF5I GGATG 3 cut(s) 838, 1096, 1401
BstKTI GATC 6 cut(s) 220, 782, 1096, 1132, 1140, 1360
BstMAI GTCTC 7 cut(s) 14, 140, 175, 504, 638, 1115, 1235
BstMBI GATC 6 cut(s) 217, 779, 1093, 1129, 1137, 1357
BstMWI GCNNNNNNNGC 3 cut(s) 102, 108, 992
BstNI CCWGG 4 cut(s) 271, 371, 1118, 1142
BstNSI RCATGY 1 cut(s) 536
BstSCI CCNGG 5 cut(s) 269, 369, 426, 1116, 1140
BstSFI CTRYAG 4 cut(s) 97, 250, 326, 639
BstSLI GKGCMC 1 cut(s) 179
BstV1I GCAGC 3 cut(s) 95, 117, 532
BstX2I RGATCY 2 cut(s) 1129, 1137
BstYI RGATCY 2 cut(s) 1129, 1137
Bsu36I CCTNAGG 1 cut(s) 1314
BsuRI GGCC 3 cut(s) 269, 759, 1336
BtsCI GGATG 3 cut(s) 838, 1096, 1401
BtsI GCAGTG 1 cut(s) 807
BtsIMutI CAGTG 1 cut(s) 807
Cac8I GCNNGC 3 cut(s) 391, 411, 1265
CciI TCATGA 1 cut(s) 622
Cfr13I GGNCC 1 cut(s) 1335
CseI GACGC 4 cut(s) 959, 1163, 1200, 1280
Csp6I GTAC 5 cut(s) 367, 644, 725, 766, 1379
CviAII CATG 4 cut(s) 533, 623, 761, 1034
CviQI GTAC 5 cut(s) 367, 644, 725, 766, 1379
DdeI CTNAG 6 cut(s) 166, 236, 522, 776, 1054, 1314
DpnI GATC 6 cut(s) 219, 781, 1095, 1131, 1139, 1359
DpnII GATC 6 cut(s) 217, 779, 1093, 1129, 1137, 1357
EaeI YGGCCR 1 cut(s) 267
EciI GGCGGA 1 cut(s) 1334
Eco31I GGTCTC 1 cut(s) 504
Eco57I CTGAAG 1 cut(s) 1040
Eco81I CCTNAGG 1 cut(s) 1314
EcoRII CCWGG 4 cut(s) 269, 369, 1116, 1140
EcoT22I ATGCAT 1 cut(s) 1009
Esp3I CGTCTC 1 cut(s) 14
FaeI CATG 4 cut(s) 536, 626, 764, 1037
FaqI GGGAC 2 cut(s) 473, 1119
FatI CATG 4 cut(s) 532, 622, 760, 1033
FauI CCCGC 1 cut(s) 34
FauNDI CATATG 1 cut(s) 802
FbaI TGATCA 2 cut(s) 1093, 1357
Fnu4HI GCNGC 5 cut(s) 54, 103, 106, 109, 546
FokI GGATG 3 cut(s) 845, 1083, 1408
Fsp4HI GCNGC 5 cut(s) 54, 103, 106, 109, 546
FspBI CTAG 2 cut(s) 936, 1109
GluI GCNGC 5 cut(s) 54, 103, 106, 109, 546
HaeIII GGCC 3 cut(s) 269, 759, 1336
HapII CCGG 1 cut(s) 427
HgaI GACGC 4 cut(s) 959, 1163, 1200, 1280
Hin1I GRCGYC 2 cut(s) 970, 1272
Hin1II CATG 4 cut(s) 536, 626, 764, 1037
HincII GTYRAC 1 cut(s) 886
HindII GTYRAC 1 cut(s) 886
HindIII AAGCTT 1 cut(s) 984
HinfI GANTC 8 cut(s) 213, 226, 305, 626, 683, 794, 1250, 1281
HpaII CCGG 1 cut(s) 427
HphI GGTGA 6 cut(s) 395, 746, 859, 1037, 1049, 1377
Hpy166II GTNNAC 5 cut(s) 177, 661, 752, 766, 886
Hpy188I TCNGA 5 cut(s) 693, 1020, 1216, 1280, 1357
Hpy188III TCNNGA 4 cut(s) 623, 918, 1135, 1169
Hpy8I GTNNAC 5 cut(s) 177, 661, 752, 766, 886
Hpy99I CGWCG 2 cut(s) 127, 1157
HpyAV CCTTC 3 cut(s) 1015, 1288, 1318
HpyCH4III ACNGT 2 cut(s) 515, 1238
HpyCH4IV ACGT 4 cut(s) 23, 576, 1049, 1287
HpyCH4V TGCA 9 cut(s) 28, 111, 177, 328, 389, 493, 652, 673, 1007
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 108, 992
HpyF3I CTNAG 6 cut(s) 166, 236, 522, 776, 1054, 1314
HpySE526I ACGT 4 cut(s) 23, 576, 1049, 1287
Hsp92I GRCGYC 2 cut(s) 970, 1272
Hsp92II CATG 4 cut(s) 536, 626, 764, 1037
Ksp22I TGATCA 2 cut(s) 1093, 1357
Kzo9I GATC 6 cut(s) 217, 779, 1093, 1129, 1137, 1357
LmnI GCTCC 3 cut(s) 93, 202, 1369
Lsp1109I GCAGC 3 cut(s) 95, 117, 532
LweI GCATC 7 cut(s) 376, 661, 705, 817, 823, 994, 1138
MaeI CTAG 2 cut(s) 936, 1109
MaeII ACGT 4 cut(s) 23, 576, 1049, 1287
MaeIII GTNAC 2 cut(s) 734, 915
MalI GATC 6 cut(s) 219, 781, 1095, 1131, 1139, 1359
MboI GATC 6 cut(s) 217, 779, 1093, 1129, 1137, 1357
MboII GAAGA 4 cut(s) 362, 1190, 1303, 1352
MflI RGATCY 2 cut(s) 1129, 1137
MhlI GDGCHC 1 cut(s) 179
MlsI TGGCCA 1 cut(s) 269
MluNI TGGCCA 1 cut(s) 269
MlyI GAGTC 3 cut(s) 207, 692, 1259
MmeI TCCRAC 1 cut(s) 322
Mox20I TGGCCA 1 cut(s) 269
Mph1103I ATGCAT 1 cut(s) 1009
MscI TGGCCA 1 cut(s) 269
MseI TTAA 6 cut(s) 63, 405, 498, 1083, 1088, 1185
MslI CAYNNNNRTG 1 cut(s) 498
Msp20I TGGCCA 1 cut(s) 269
MspA1I CMGCKG 2 cut(s) 102, 108
MspI CCGG 1 cut(s) 427
MspR9I CCNGG 5 cut(s) 271, 371, 428, 1118, 1142
MvaI CCWGG 4 cut(s) 271, 371, 1118, 1142
MwoI GCNNNNNNNGC 3 cut(s) 102, 108, 992
NciI CCSGG 1 cut(s) 428
NdeI CATATG 1 cut(s) 802
NdeII GATC 6 cut(s) 217, 779, 1093, 1129, 1137, 1357
NlaIII CATG 4 cut(s) 536, 626, 764, 1037
NlaIV GGNNCC 3 cut(s) 198, 275, 1139
NmuCI GTSAC 2 cut(s) 734, 915
NsiI ATGCAT 1 cut(s) 1009
NspI RCATGY 1 cut(s) 536
PagI TCATGA 1 cut(s) 622
PciI ACATGT 1 cut(s) 532
PcsI WCGNNNNNNNCGW 2 cut(s) 122, 1284
PfeI GAWTC 5 cut(s) 226, 305, 626, 794, 1281
PflFI GACNNNGTC 1 cut(s) 1210
PfoI TCCNGGA 1 cut(s) 1116
PkrI GCNGC 5 cut(s) 55, 104, 107, 110, 547
PleI GAGTC 3 cut(s) 207, 691, 1258
PpsI GAGTC 3 cut(s) 207, 691, 1258
PscI ACATGT 1 cut(s) 532
PshBI ATTAAT 1 cut(s) 63
Psp1406I AACGTT 1 cut(s) 576
Psp6I CCWGG 4 cut(s) 269, 369, 1116, 1140
PspGI CCWGG 4 cut(s) 269, 369, 1116, 1140
PspN4I GGNNCC 3 cut(s) 198, 275, 1139
PspPI GGNCC 1 cut(s) 1335
PstI CTGCAG 1 cut(s) 330
PsuI RGATCY 2 cut(s) 1129, 1137
PsyI GACNNNGTC 1 cut(s) 1210
PvuII CAGCTG 1 cut(s) 108
RsaI GTAC 5 cut(s) 368, 645, 726, 767, 1380
RsaNI GTAC 5 cut(s) 367, 644, 725, 766, 1379
RseI CAYNNNNRTG 1 cut(s) 498
SaqAI TTAA 6 cut(s) 63, 405, 498, 1083, 1088, 1185
SatI GCNGC 5 cut(s) 54, 103, 106, 109, 546
Sau3AI GATC 6 cut(s) 217, 779, 1093, 1129, 1137, 1357
Sau96I GGNCC 1 cut(s) 1335
SchI GAGTC 3 cut(s) 207, 692, 1259
ScrFI CCNGG 5 cut(s) 271, 371, 428, 1118, 1142
SduI GDGCHC 1 cut(s) 179
SfaNI GCATC 7 cut(s) 376, 661, 705, 817, 823, 994, 1138
SfcI CTRYAG 4 cut(s) 97, 250, 326, 639
SmiMI CAYNNNNRTG 1 cut(s) 498
SpeI ACTAGT 1 cut(s) 935
SsiI CCGC 7 cut(s) 41, 53, 102, 830, 856, 879, 1319
SspI AATATT 2 cut(s) 190, 963
SspMI CTAG 2 cut(s) 936, 1109
StyD4I CCNGG 5 cut(s) 269, 369, 426, 1116, 1140
TaaI ACNGT 2 cut(s) 515, 1238
TaiI ACGT 4 cut(s) 26, 579, 1052, 1290
TaqI TCGA 4 cut(s) 125, 216, 989, 1205
TatI WGTACW 2 cut(s) 765, 1378
TauI GCSGC 2 cut(s) 56, 105
TfiI GAWTC 5 cut(s) 226, 305, 626, 794, 1281
Tru1I TTAA 6 cut(s) 63, 405, 498, 1083, 1088, 1185
Tru9I TTAA 6 cut(s) 63, 405, 498, 1083, 1088, 1185
TscAI CASTG 1 cut(s) 814
TseFI GTSAC 2 cut(s) 734, 915
TseI GCWGC 3 cut(s) 105, 108, 545
Tsp45I GTSAC 2 cut(s) 734, 915
TspDTI ATGAA 3 cut(s) 363, 711, 880
TspRI CASTG 1 cut(s) 814
Tth111I GACNNNGTC 1 cut(s) 1210
VneI GTGCAC 1 cut(s) 175
VspI ATTAAT 1 cut(s) 63
XapI RAATTY 6 cut(s) 13, 353, 666, 770, 1181, 1194
XceI RCATGY 1 cut(s) 536
XcmI CCANNNNNNNNNTGG 1 cut(s) 1395
XspI CTAG 2 cut(s) 936, 1109
Zsp2I ATGCAT 1 cut(s) 1009
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.