pycom07g16020

Protein of unknown function (DUF1295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006054
Physical Location & Seq
Reverse (-)
18521137 .. 18522489
1353 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1035 bp
ATGAACTATAAAACTAGGCCTCTATTGTCTATTCCTTCATTGGTAATTGGATTTACTGCGGCCATGGCTGGAGGCAACTTGAAGAATGCAGTGATAGCATTTCTCGCTCCCCTTCCCTCCATTCTCTTCTACCTCATATTCCTCAGCAACCACCATAGCCAAACTAATGAGAGCTTCCTCTCTCCTCTCTGGACATGGTGCTATCACCACCCTCTTCTCCTAGCCAACGTTTTCTTCTTCCTCAACGTGAACGTCCTCTTCTGGGTCATCAGCCAAGCTCTTTCCAGCCACTGGATGATCGATTTGTACTGGACTGTGATCCCTGTAATGCTCGTTCACTACTATGGATCTCACCCTCTGGCTGATTACAACCGGTGGCGGTCGACGATTGCAATTTCGCTGACTTGGGTTTGGAGCCTCAGGCTTAGCCACAACTACTTTAGGCGGGAAAATTGGCAATGGGGTGCCAGGGAGGACTGGCGGTTCACAGATATGAGCTCACAGTACGGCAAACATTGGTGGTGGGTTTCCTTCTTCGCCGTTTACCTCTCTCAACAGGTTTTTATAATTGGGATATGCCTTCCATTCTATGTAATCCACTCAGTTAATGTGCCACTGAACATATGGGATTTTGTTGCCGTCGTAGTCTGCATTACTGGCATTGTTGTAGCATACTTAGCTGACACGCAGCTCCATGACTTTGTGAACCGAAACAACAAGCTGAAAGAGCTTGGAAAGCCCGTTGTTCCGAATCTTGACGAAGGGTTGTGGCACTATTCGCGGCATCCCAACTACTTCGGAGAGCAATTGTGGTGGTGGGGATTAGTCATATTTGCATGGAACTTGGGACATGGATGGGCCTTTGTTGGGTCTCTGGTCAATACAATGTGTTTAGCTTATGTGACTATACTTGTCGAACGCCGAATGCTGAAACAAGAATACCGAGTAGAAGCATACAGGCGGTATCAGAAGACAACCTCAGTGTGGATACCTTGGTTCAAGTCATCTCCAGGAGGAAAAGATAAGAACGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0006629 GO:0008150 GO:0008152 GO:0008202 GO:0016229 GO:0016491 GO:0044238 GO:0055114 GO:0071704 GO:1901360
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1295

Protein Analysis

345

Amino Acids

40.5

Weight (kDa)

9.25

Isoelectric Point (pI)

38.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1295 PF06966 87 - 326 8.8e-65 Protein of unknown function (DUF1295)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 566
AccB1I GGYRCC 1 cut(s) 464
AccB7I CCANNNNNTGG 1 cut(s) 291
AccI GTMKAC 1 cut(s) 383
AccII CGCG 1 cut(s) 781
AciI CCGC 6 cut(s) 59, 379, 445, 481, 781, 961
AclI AACGTT 1 cut(s) 228
AclWI GGATC 2 cut(s) 313, 355
AcoI YGGCCR 1 cut(s) 60
AfaI GTAC 2 cut(s) 308, 506
AfiI CCNNNNNNNGG 4 cut(s) 262, 291, 867, 984
AgeI ACCGGT 1 cut(s) 372
AgsI TTSAA 2 cut(s) 82, 1000
AjnI CCWGG 2 cut(s) 467, 1009
AjuI GAANNNNNNNTTGG 2 cut(s) 218, 250
AloI GAACNNNNNNTCC 2 cut(s) 467, 499
AluBI AGCT 8 cut(s) 174, 278, 498, 680, 691, 721, 730, 896
AluI AGCT 8 cut(s) 174, 278, 498, 680, 691, 721, 730, 896
Alw21I GWGCWC 1 cut(s) 500
Alw26I GTCTC 1 cut(s) 876
AlwI GGATC 2 cut(s) 313, 355
AlwNI CAGNNNCTG 1 cut(s) 291
AoxI GGCC 3 cut(s) 17, 60, 858
ApeKI GCWGC 1 cut(s) 688
AsiGI ACCGGT 1 cut(s) 372
AspS9I GGNCC 1 cut(s) 858
AsuHPI GGTGA 2 cut(s) 197, 344
AxyI CCTNAGG 1 cut(s) 419
BaeI ACNNNNGTAYC 2 cut(s) 980, 1013
BanI GGYRCC 1 cut(s) 464
BanII GRGCYC 1 cut(s) 500
BbsI GAAGAC 1 cut(s) 977
Bbv12I GWGCWC 1 cut(s) 500
BbvCI CCTCAGC 1 cut(s) 143
BbvI GCAGC 1 cut(s) 700
BccI CCATC 1 cut(s) 849
BceAI ACGGC 3 cut(s) 523, 524, 623
BciT130I CCWGG 2 cut(s) 469, 1011
BciVI GTATCC 1 cut(s) 981
BcoDI GTCTC 1 cut(s) 876
BfaI CTAG 2 cut(s) 15, 221
BfuI GTATCC 1 cut(s) 981
BisI GCNGC 3 cut(s) 60, 689, 782
BlpI GCTNAGC 1 cut(s) 425
BlsI GCNGC 3 cut(s) 61, 690, 783
Bme1390I CCNGG 2 cut(s) 469, 1011
BmgT120I GGNCC 1 cut(s) 858
BmiI GGNNCC 2 cut(s) 416, 466
BmrFI CCNGG 2 cut(s) 469, 1011
BmsI GCATC 1 cut(s) 793
BpiI GAAGAC 1 cut(s) 977
BpmI CTGGAG 2 cut(s) 90, 993
Bpu10I CCTNAGC 1 cut(s) 143
Bpu1102I GCTNAGC 1 cut(s) 425
Bsa29I ATCGAT 1 cut(s) 300
BsaI GGTCTC 1 cut(s) 876
BsaJI CCNNGG 3 cut(s) 63, 468, 992
BsaWI WCCGGW 1 cut(s) 372
Bsc4I CCNNNNNNNGG 4 cut(s) 262, 291, 867, 984
Bse118I RCCGGY 1 cut(s) 372
Bse1I ACTGG 4 cut(s) 296, 314, 482, 661
Bse21I CCTNAGG 1 cut(s) 419
Bse3DI GCAATG 1 cut(s) 464
BseBI CCWGG 2 cut(s) 469, 1011
BseCI ATCGAT 1 cut(s) 300
BseDI CCNNGG 3 cut(s) 63, 468, 992
BseGI GGATG 3 cut(s) 300, 784, 860
BseLI CCNNNNNNNGG 4 cut(s) 262, 291, 867, 984
BseMI GCAATG 1 cut(s) 464
BseMII CTCAG 4 cut(s) 157, 433, 615, 993
BseNI ACTGG 4 cut(s) 296, 314, 482, 661
BseRI GAGGAG 1 cut(s) 174
BseXI GCAGC 1 cut(s) 700
Bsh1236I CGCG 1 cut(s) 781
Bsh1285I CGRYCG 1 cut(s) 383
BshFI GGCC 3 cut(s) 19, 62, 860
BshNI GGYRCC 1 cut(s) 464
BshTI ACCGGT 1 cut(s) 372
BshVI ATCGAT 1 cut(s) 300
BsiEI CGRYCG 1 cut(s) 383
BsiHKAI GWGCWC 1 cut(s) 500
BsiSI CCGG 1 cut(s) 373
BslFI GGGAC 1 cut(s) 861
BslI CCNNNNNNNGG 4 cut(s) 262, 291, 867, 984
BsmAI GTCTC 1 cut(s) 876
BsmFI GGGAC 1 cut(s) 861
BsmI GAATGC 2 cut(s) 91, 930
BsnI GGCC 3 cut(s) 19, 62, 860
Bso31I GGTCTC 1 cut(s) 876
Bsp1286I GDGCHC 1 cut(s) 500
Bsp143I GATC 3 cut(s) 297, 318, 347
Bsp1720I GCTNAGC 1 cut(s) 425
Bsp19I CCATGG 1 cut(s) 63
BspACI CCGC 6 cut(s) 59, 379, 445, 481, 781, 961
BspANI GGCC 3 cut(s) 19, 62, 860
BspCNI CTCAG 4 cut(s) 156, 432, 614, 992
BspDI ATCGAT 1 cut(s) 300
BspFNI CGCG 1 cut(s) 781
BspLI GGNNCC 2 cut(s) 416, 466
BspPI GGATC 2 cut(s) 313, 355
BspT107I GGYRCC 1 cut(s) 464
BspTNI GGTCTC 1 cut(s) 876
BsrDI GCAATG 1 cut(s) 464
BsrFI RCCGGY 1 cut(s) 372
BsrI ACTGG 4 cut(s) 296, 314, 482, 661
BssAI RCCGGY 1 cut(s) 372
BssECI CCNNGG 3 cut(s) 63, 468, 992
BssMI GATC 3 cut(s) 297, 318, 347
BssT1I CCWWGG 2 cut(s) 63, 992
Bst2UI CCWGG 2 cut(s) 469, 1011
Bst4CI ACNGT 2 cut(s) 316, 504
Bst6I CTCTTC 3 cut(s) 131, 219, 263
BstDEI CTNAG 6 cut(s) 143, 419, 425, 601, 676, 979
BstDSI CCRYGG 1 cut(s) 63
BstF5I GGATG 3 cut(s) 300, 784, 860
BstFNI CGCG 1 cut(s) 781
BstKTI GATC 3 cut(s) 300, 321, 350
BstMAI GTCTC 1 cut(s) 876
BstMBI GATC 3 cut(s) 297, 318, 347
BstMCI CGRYCG 1 cut(s) 383
BstMWI GCNNNNNNNGC 8 cut(s) 65, 95, 104, 657, 677, 727, 736, 778
BstNI CCWGG 2 cut(s) 469, 1011
BstSCI CCNGG 2 cut(s) 467, 1009
BstUI CGCG 1 cut(s) 781
BstV1I GCAGC 1 cut(s) 700
BstV2I GAAGAC 1 cut(s) 977
BstX2I RGATCY 1 cut(s) 347
BstYI RGATCY 1 cut(s) 347
Bsu15I ATCGAT 1 cut(s) 300
Bsu36I CCTNAGG 1 cut(s) 419
BsuI GTATCC 1 cut(s) 981
BsuRI GGCC 3 cut(s) 19, 62, 860
BsuTUI ATCGAT 1 cut(s) 300
BtgI CCRYGG 1 cut(s) 63
BtsCI GGATG 3 cut(s) 300, 784, 860
BtsI GCAGTG 1 cut(s) 96
BtsIMutI CAGTG 4 cut(s) 96, 289, 614, 987
CaiI CAGNNNCTG 1 cut(s) 291
Cfr10I RCCGGY 1 cut(s) 372
Cfr13I GGNCC 1 cut(s) 858
ClaI ATCGAT 1 cut(s) 300
Csp6I GTAC 2 cut(s) 307, 505
CspAI ACCGGT 1 cut(s) 372
CspCI CAANNNNNGTGG 2 cut(s) 794, 829
CviAII CATG 5 cut(s) 64, 195, 695, 837, 851
CviQI GTAC 2 cut(s) 307, 505
DdeI CTNAG 6 cut(s) 143, 419, 425, 601, 676, 979
DpnI GATC 3 cut(s) 299, 320, 349
DpnII GATC 3 cut(s) 297, 318, 347
EaeI YGGCCR 1 cut(s) 60
Eam1104I CTCTTC 3 cut(s) 131, 219, 263
EarI CTCTTC 3 cut(s) 131, 219, 263
Ecl136II GAGCTC 1 cut(s) 498
Eco130I CCWWGG 2 cut(s) 63, 992
Eco147I AGGCCT 1 cut(s) 19
Eco24I GRGCYC 1 cut(s) 500
Eco31I GGTCTC 1 cut(s) 876
Eco53kI GAGCTC 1 cut(s) 498
Eco81I CCTNAGG 1 cut(s) 419
EcoICRI GAGCTC 1 cut(s) 498
EcoRII CCWGG 2 cut(s) 467, 1009
EcoT14I CCWWGG 2 cut(s) 63, 992
EcoT38I GRGCYC 1 cut(s) 500
ErhI CCWWGG 2 cut(s) 63, 992
FaeI CATG 5 cut(s) 67, 198, 698, 840, 854
FaqI GGGAC 1 cut(s) 861
FatI CATG 5 cut(s) 63, 194, 694, 836, 850
FauI CCCGC 1 cut(s) 438
FauNDI CATATG 1 cut(s) 623
FblI GTMKAC 1 cut(s) 383
Fnu4HI GCNGC 3 cut(s) 60, 689, 782
FokI GGATG 3 cut(s) 307, 771, 867
FriOI GRGCYC 1 cut(s) 500
Fsp4HI GCNGC 3 cut(s) 60, 689, 782
FspBI CTAG 2 cut(s) 15, 221
GluI GCNGC 3 cut(s) 60, 689, 782
GsuI CTGGAG 2 cut(s) 90, 993
HaeIII GGCC 3 cut(s) 19, 62, 860
HapII CCGG 1 cut(s) 373
Hin1II CATG 5 cut(s) 67, 198, 698, 840, 854
HincII GTYRAC 1 cut(s) 384
HindII GTYRAC 1 cut(s) 384
HinfI GANTC 1 cut(s) 751
HpaII CCGG 1 cut(s) 373
HphI GGTGA 2 cut(s) 197, 344
Hpy166II GTNNAC 6 cut(s) 250, 337, 384, 486, 544, 706
Hpy188I TCNGA 3 cut(s) 750, 800, 969
Hpy188III TCNNGA 2 cut(s) 190, 755
Hpy8I GTNNAC 6 cut(s) 250, 337, 384, 486, 544, 706
Hpy99I CGWCG 2 cut(s) 388, 644
HpyAV CCTTC 5 cut(s) 45, 122, 541, 590, 755
HpyCH4III ACNGT 2 cut(s) 316, 504
HpyCH4IV ACGT 3 cut(s) 228, 246, 252
HpyCH4V TGCA 4 cut(s) 89, 392, 651, 836
HpyF10VI GCNNNNNNNGC 8 cut(s) 65, 95, 104, 657, 677, 727, 736, 778
HpyF3I CTNAG 6 cut(s) 143, 419, 425, 601, 676, 979
HpySE526I ACGT 3 cut(s) 228, 246, 252
Hsp92II CATG 5 cut(s) 67, 198, 698, 840, 854
Kzo9I GATC 3 cut(s) 297, 318, 347
LmnI GCTCC 3 cut(s) 112, 414, 696
Lsp1109I GCAGC 1 cut(s) 700
LweI GCATC 1 cut(s) 793
MaeI CTAG 2 cut(s) 15, 221
MaeII ACGT 3 cut(s) 228, 246, 252
MaeIII GTNAC 1 cut(s) 901
MalI GATC 3 cut(s) 299, 320, 349
MboI GATC 3 cut(s) 297, 318, 347
MboII GAAGA 8 cut(s) 94, 118, 206, 226, 229, 250, 526, 982
MfeI CAATTG 1 cut(s) 806
MflI RGATCY 1 cut(s) 347
MhlI GDGCHC 1 cut(s) 500
MluCI AATT 5 cut(s) 45, 393, 451, 567, 806
MseI TTAA 2 cut(s) 606, 1033
MslI CAYNNNNRTG 2 cut(s) 342, 491
MspI CCGG 1 cut(s) 373
MspR9I CCNGG 2 cut(s) 469, 1011
MunI CAATTG 1 cut(s) 806
Mva1269I GAATGC 2 cut(s) 91, 930
MvaI CCWGG 2 cut(s) 469, 1011
MvnI CGCG 1 cut(s) 781
MwoI GCNNNNNNNGC 8 cut(s) 65, 95, 104, 657, 677, 727, 736, 778
NcoI CCATGG 1 cut(s) 63
NdeI CATATG 1 cut(s) 623
NdeII GATC 3 cut(s) 297, 318, 347
NlaIII CATG 5 cut(s) 67, 198, 698, 840, 854
NlaIV GGNNCC 2 cut(s) 416, 466
NmuCI GTSAC 1 cut(s) 901
PceI AGGCCT 1 cut(s) 19
PctI GAATGC 2 cut(s) 91, 930
PfeI GAWTC 1 cut(s) 751
PflMI CCANNNNNTGG 1 cut(s) 291
PfoI TCCNGGA 1 cut(s) 1009
PinAI ACCGGT 1 cut(s) 372
PkrI GCNGC 3 cut(s) 61, 690, 783
PsiI TTATAA 1 cut(s) 566
Psp124BI GAGCTC 1 cut(s) 500
Psp1406I AACGTT 1 cut(s) 228
Psp6I CCWGG 2 cut(s) 467, 1009
PspGI CCWGG 2 cut(s) 467, 1009
PspN4I GGNNCC 2 cut(s) 416, 466
PspPI GGNCC 1 cut(s) 858
PsrI GAACNNNNNNTAC 2 cut(s) 318, 350
PstNI CAGNNNCTG 1 cut(s) 291
PsuI RGATCY 1 cut(s) 347
RsaI GTAC 2 cut(s) 308, 506
RsaNI GTAC 2 cut(s) 307, 505
RseI CAYNNNNRTG 2 cut(s) 342, 491
SacI GAGCTC 1 cut(s) 500
SalI GTCGAC 1 cut(s) 382
SaqAI TTAA 2 cut(s) 606, 1033
SatI GCNGC 3 cut(s) 60, 689, 782
Sau3AI GATC 3 cut(s) 297, 318, 347
Sau96I GGNCC 1 cut(s) 858
ScrFI CCNGG 2 cut(s) 469, 1011
SduI GDGCHC 1 cut(s) 500
SfaNI GCATC 1 cut(s) 793
SmiMI CAYNNNNRTG 2 cut(s) 342, 491
Sse9I AATT 5 cut(s) 45, 393, 451, 567, 806
SseBI AGGCCT 1 cut(s) 19
SsiI CCGC 6 cut(s) 59, 379, 445, 481, 781, 961
SspMI CTAG 2 cut(s) 15, 221
SstI GAGCTC 1 cut(s) 500
StuI AGGCCT 1 cut(s) 19
StyD4I CCNGG 2 cut(s) 467, 1009
StyI CCWWGG 2 cut(s) 63, 992
TaaI ACNGT 2 cut(s) 316, 504
TaiI ACGT 3 cut(s) 231, 249, 255
TaqI TCGA 3 cut(s) 300, 383, 915
TasI AATT 5 cut(s) 45, 393, 451, 567, 806
TatI WGTACW 1 cut(s) 306
TauI GCSGC 2 cut(s) 62, 784
TfiI GAWTC 1 cut(s) 751
Tru1I TTAA 2 cut(s) 606, 1033
Tru9I TTAA 2 cut(s) 606, 1033
TscAI CASTG 4 cut(s) 96, 296, 621, 987
TseFI GTSAC 1 cut(s) 901
TseI GCWGC 1 cut(s) 688
Tsp45I GTSAC 1 cut(s) 901
TspDTI ATGAA 2 cut(s) 17, 27
TspRI CASTG 4 cut(s) 96, 296, 621, 987
Van91I CCANNNNNTGG 1 cut(s) 291
XcmI CCANNNNNNNNNTGG 1 cut(s) 621
XmiI GTMKAC 1 cut(s) 383
XspI CTAG 2 cut(s) 15, 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.