pycom07g18640

Dual-specificity RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0010937
Physical Location & Seq
Forward (+)
21087210 .. 21089845
2636 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1365 bp
ATGGGATCGATCCAACTTGGGCCTAAACCACACCACGCTCTTTCTTCCTCTGCTTCATCTTCTTCATCTCGCGCGAAGCCAAAAACCCAATACAGCACAATGATGACAGCCTCATCAACATCGCTAATTCAGCGCGTCTGCGCCGTTCCTCTCGCACGCGCGGCCCGCACTCGCGCCATGGCAGCGGCGAGCATGAACCTGTCCACCACCACCTCCACCACCACCACCACCGCTCCCTCCCTAGCGAAACGGGCCGACCCCCAAGTCCTCCTAGGCTTGTCGGAACCGGAGCTCCAGCAGCTCGCAATCGACTTCAGTCAGCAAGGCTACAGAGGGAAGCAGCTCCATCATCTAATTTACAAAAGAAAGATTAAAGATATTCAGGATTTCAATCAGGTGCCTCTGGCATTCAGAAATGAGCTTGAGGAAGCTGGATGGAAAGTTGGGAGGTCACCCATTTACCAGAGCGTCACTGCTGCTGATGGCACTGTCAAGTTGTTGATAAAGTTGGAGGATAACAGATTGATTGAAACTGTTGGCATACCAGTTAAAGATGAGAAAGGTGTGATGCGCCTTACAGCGTGTGTCTCATCACAGGTGGGATGCCCTCTTCGTTGCTCTTTTTGTGCCACGGGAAAAGGAGGATTTTCAAGAAACCTCAAGAGGCATGAAATTGTTGAGCAGGTTTTGGCTATTGAGGAAATCTTCAACCATAGGGTGACTAATGTGGTGTTCATGGGAATGGGTGAACCGATGATGAACCTGAAGGCGGTTCTTGAAGCGCATCAGTGTTTAAACAAGGATATTCAAATTGGGCAAAGAATGATCACAATTTCTACGGTGGGGGTTCCAAATACAATTAAAAAGCTGGCATCCCACAAACTTCAGTCGACATTGGCTGTCAGCCTGCATGCTCCTAACCAGAAGCTGAGGGAAACAATTGTGCCAAGTGCAAAAGCATACCCTCTGAGTGCACTGATGAGGGATTGCAGGGACTATTTCACTGAAACCGGTAGACGGGTTTCCTTTGAGTATGCACTCTTAGCTGGAGTCAATGATGCGGTAGACCACGCAGTAGAACTTGCAGAGCTACTCCATGAGTGGGGACGAGGTTCTCATGTGAACCTGATACCATTTAATCCAATAGAAGGCTCTGAGTATAAACGTCCATCCAAGAAAGCGGTACAAGCATTTGCAGCTGCTCTGGAGAACAATAAAGTAACGGTCAGCACACGTCAAACAAGGGGCTTGGATGCAGATGCAGCTTGTGGTCAGCTAAGAAACAAGTTCCAGAAGAGTCCTTTGCCCGTTGACTCTGATAACTTACAAACTGATGAAGATGCTCCAGTTGCAGTTGCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

455

Amino Acids

49.59

Weight (kDa)

9.3

Isoelectric Point (pI)

42.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 197 - 361 5.6e-16 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010937)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39670 AT2G39670
fragaria_vesca FvH4_7g22600
malus_domestica MD07G1012700.v1.1 MD07G1199600.v1.1
prunus_persica Prupe.2G236300_v2.0.a1
pyrus_communis pycom07g01040 pycom07g18640
rosa_chinensis RchiOBHm_Chr1g0367701
rosa_laevigata RLG00000027269
rosa_multiflora Rmu_sc0008470.1_g000011 Rmu_sc0022951.1_g000001 Rmu_sc0027455.1_g000001
rosa_roxburghii Rroxscaffold_4G00289290
rosa_rugosa Rorug01G0336200
rosa_samantha Rh1AG343200 Rh1BG304900 Rh1CG320600 Rh1DG337200
rosa_wichuraiana Rw1G030540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 263
Acc36I ACCTGC 1 cut(s) 673
AccB1I GGYRCC 1 cut(s) 397
AccBSI CCGCTC 1 cut(s) 233
AccI GTMKAC 3 cut(s) 890, 1015, 1065
AccII CGCG 6 cut(s) 72, 74, 135, 159, 161, 174
AciI CCGC 7 cut(s) 161, 166, 185, 231, 770, 1061, 1181
AclWI GGATC 2 cut(s) 4, 13
AcuI CTGAAG 3 cut(s) 298, 785, 869
AfaI GTAC 1 cut(s) 1185
AfiI CCNNNNNNNGG 4 cut(s) 769, 1017, 1102, 1148
AflIII ACRYGT 1 cut(s) 1232
AgeI ACCGGT 1 cut(s) 1010
AgsI TTSAA 6 cut(s) 391, 530, 651, 709, 779, 809
AjiI CACGTC 1 cut(s) 1235
AloI GAACNNNNNNTCC 2 cut(s) 276, 308
Alw21I GWGCWC 2 cut(s) 294, 976
Alw26I GTCTC 1 cut(s) 592
Alw44I GTGCAC 1 cut(s) 972
AlwI GGATC 2 cut(s) 4, 13
AlwNI CAGNNNCTG 1 cut(s) 928
AoxI GGCC 3 cut(s) 20, 162, 252
ApaLI GTGCAC 1 cut(s) 972
ApeKI GCWGC 7 cut(s) 182, 298, 340, 476, 1196, 1199, 1262
AsiGI ACCGGT 1 cut(s) 1010
AspA2I CCTAGG 1 cut(s) 271
AspLEI GCGC 7 cut(s) 74, 135, 143, 161, 176, 573, 784
AspS9I GGNCC 3 cut(s) 20, 163, 252
AsuHPI GGTGA 3 cut(s) 444, 730, 758
AvrII CCTAGG 1 cut(s) 271
BaeGI GKGCMC 1 cut(s) 976
BanI GGYRCC 1 cut(s) 397
BanII GRGCYC 1 cut(s) 294
Bbv12I GWGCWC 2 cut(s) 294, 976
BbvCI CCTCAGC 1 cut(s) 929
BbvI GCAGC 7 cut(s) 194, 310, 352, 463, 1186, 1208, 1274
BccI CCATC 4 cut(s) 354, 429, 476, 1177
BceAI ACGGC 1 cut(s) 128
BclI TGATCA 1 cut(s) 825
BcoDI GTCTC 1 cut(s) 592
BfaI CTAG 2 cut(s) 242, 272
BfmI CTRYAG 1 cut(s) 328
BfuAI ACCTGC 1 cut(s) 673
BisI GCNGC 9 cut(s) 162, 183, 186, 299, 341, 477, 1197, 1200, 1263
BlnI CCTAGG 1 cut(s) 271
BlsI GCNGC 9 cut(s) 163, 184, 187, 300, 342, 478, 1198, 1201, 1264
BmgBI CACGTC 1 cut(s) 1235
BmgT120I GGNCC 3 cut(s) 20, 163, 252
BmiI GGNNCC 3 cut(s) 285, 399, 849
BmsI GCATC 8 cut(s) 558, 593, 793, 881, 1048, 1243, 1249, 1330
BoxI GACNNNNGTC 1 cut(s) 315
BpmI CTGGAG 4 cut(s) 278, 1068, 1226, 1329
Bpu10I CCTNAGC 1 cut(s) 929
BpuEI CTTGAG 2 cut(s) 443, 644
Bsa29I ATCGAT 1 cut(s) 8
BsaBI GATNNNNATC 1 cut(s) 390
BsaJI CCNNGG 3 cut(s) 177, 271, 630
BsaWI WCCGGW 2 cut(s) 286, 1010
BsaXI ACNNNNNCTCC 4 cut(s) 217, 247, 276, 306
Bsc4I CCNNNNNNNGG 4 cut(s) 769, 1017, 1102, 1148
Bse118I RCCGGY 1 cut(s) 1010
Bse1I ACTGG 2 cut(s) 545, 1346
Bse8I GATNNNNATC 1 cut(s) 390
BseCI ATCGAT 1 cut(s) 8
BseDI CCNNGG 3 cut(s) 177, 271, 630
BseGI GGATG 5 cut(s) 440, 608, 872, 1169, 1258
BseJI GATNNNNATC 1 cut(s) 390
BseLI CCNNNNNNNGG 4 cut(s) 769, 1017, 1102, 1148
BseMII CTCAG 3 cut(s) 920, 959, 1146
BseNI ACTGG 2 cut(s) 545, 1346
BseSI GKGCMC 1 cut(s) 976
BseXI GCAGC 7 cut(s) 194, 310, 352, 463, 1186, 1208, 1274
Bsh1236I CGCG 6 cut(s) 72, 74, 135, 159, 161, 174
BshFI GGCC 3 cut(s) 22, 164, 254
BshNI GGYRCC 1 cut(s) 397
BshTI ACCGGT 1 cut(s) 1010
BshVI ATCGAT 1 cut(s) 8
BsiHKAI GWGCWC 2 cut(s) 294, 976
BsiSI CCGG 2 cut(s) 287, 1011
BslFI GGGAC 2 cut(s) 1007, 1119
BslI CCNNNNNNNGG 4 cut(s) 769, 1017, 1102, 1148
BsmAI GTCTC 1 cut(s) 592
BsmFI GGGAC 2 cut(s) 1007, 1119
BsmI GAATGC 1 cut(s) 407
BsnI GGCC 3 cut(s) 22, 164, 254
Bsp1286I GDGCHC 2 cut(s) 294, 976
Bsp143I GATC 3 cut(s) 5, 9, 825
Bsp19I CCATGG 1 cut(s) 177
BspACI CCGC 7 cut(s) 161, 166, 185, 231, 770, 1061, 1181
BspANI GGCC 3 cut(s) 22, 164, 254
BspCNI CTCAG 3 cut(s) 921, 960, 1147
BspDI ATCGAT 1 cut(s) 8
BspFNI CGCG 6 cut(s) 72, 74, 135, 159, 161, 174
BspLI GGNNCC 3 cut(s) 285, 399, 849
BspMI ACCTGC 1 cut(s) 673
BspPI GGATC 2 cut(s) 4, 13
BspT107I GGYRCC 1 cut(s) 397
BsrBI CCGCTC 1 cut(s) 233
BsrFI RCCGGY 1 cut(s) 1010
BsrI ACTGG 2 cut(s) 545, 1346
BssAI RCCGGY 1 cut(s) 1010
BssECI CCNNGG 3 cut(s) 177, 271, 630
BssMI GATC 3 cut(s) 5, 9, 825
BssT1I CCWWGG 2 cut(s) 177, 271
Bst4CI ACNGT 4 cut(s) 490, 535, 841, 1225
Bst6I CTCTTC 2 cut(s) 615, 1289
BstC8I GCNNGC 7 cut(s) 157, 166, 190, 303, 870, 908, 912
BstDEI CTNAG 5 cut(s) 929, 968, 1042, 1155, 1277
BstDSI CCRYGG 2 cut(s) 177, 630
BstEII GGTNACC 1 cut(s) 450
BstF5I GGATG 5 cut(s) 440, 608, 872, 1169, 1258
BstFNI CGCG 6 cut(s) 72, 74, 135, 159, 161, 174
BstHHI GCGC 7 cut(s) 74, 135, 143, 161, 176, 573, 784
BstKTI GATC 3 cut(s) 8, 12, 828
BstMAI GTCTC 1 cut(s) 592
BstMBI GATC 3 cut(s) 5, 9, 825
BstNSI RCATGY 2 cut(s) 914, 1362
BstPAI GACNNNNGTC 1 cut(s) 315
BstPI GGTNACC 1 cut(s) 450
BstSFI CTRYAG 1 cut(s) 328
BstSLI GKGCMC 1 cut(s) 976
BstUI CGCG 6 cut(s) 72, 74, 135, 159, 161, 174
BstV1I GCAGC 7 cut(s) 194, 310, 352, 463, 1186, 1208, 1274
Bsu15I ATCGAT 1 cut(s) 8
BsuRI GGCC 3 cut(s) 22, 164, 254
BsuTUI ATCGAT 1 cut(s) 8
BtgI CCRYGG 2 cut(s) 177, 630
BtgZI GCGATG 1 cut(s) 105
BtrI CACGTC 1 cut(s) 1235
BtsCI GGATG 5 cut(s) 440, 608, 872, 1169, 1258
BtsI GCAGTG 1 cut(s) 471
BtsIMutI CAGTG 5 cut(s) 471, 486, 794, 974, 1002
BveI ACCTGC 1 cut(s) 673
Cac8I GCNNGC 7 cut(s) 157, 166, 190, 303, 870, 908, 912
CaiI CAGNNNCTG 1 cut(s) 928
CfoI GCGC 7 cut(s) 74, 135, 143, 161, 176, 573, 784
Cfr10I RCCGGY 1 cut(s) 1010
Cfr13I GGNCC 3 cut(s) 20, 163, 252
ClaI ATCGAT 1 cut(s) 8
CseI GACGC 2 cut(s) 124, 457
Csp6I GTAC 1 cut(s) 1184
CspAI ACCGGT 1 cut(s) 1010
CviAII CATG 8 cut(s) 178, 193, 668, 736, 911, 1097, 1118, 1359
CviQI GTAC 1 cut(s) 1184
DdeI CTNAG 5 cut(s) 929, 968, 1042, 1155, 1277
DpnI GATC 3 cut(s) 7, 11, 827
DpnII GATC 3 cut(s) 5, 9, 825
DraI TTTAAA 1 cut(s) 795
DrdI GACNNNNNNGTC 1 cut(s) 263
DseDI GACNNNNNNGTC 1 cut(s) 263
Eam1104I CTCTTC 2 cut(s) 615, 1289
EarI CTCTTC 2 cut(s) 615, 1289
Ecl136II GAGCTC 1 cut(s) 292
Eco130I CCWWGG 2 cut(s) 177, 271
Eco24I GRGCYC 1 cut(s) 294
Eco53kI GAGCTC 1 cut(s) 292
Eco57I CTGAAG 3 cut(s) 298, 785, 869
Eco91I GGTNACC 1 cut(s) 450
EcoICRI GAGCTC 1 cut(s) 292
EcoO65I GGTNACC 1 cut(s) 450
EcoT14I CCWWGG 2 cut(s) 177, 271
EcoT38I GRGCYC 1 cut(s) 294
ErhI CCWWGG 2 cut(s) 177, 271
FaeI CATG 8 cut(s) 181, 196, 671, 739, 914, 1100, 1121, 1362
FaqI GGGAC 2 cut(s) 1007, 1119
FatI CATG 8 cut(s) 177, 192, 667, 735, 910, 1096, 1117, 1358
FauI CCCGC 1 cut(s) 173
FbaI TGATCA 1 cut(s) 825
FblI GTMKAC 3 cut(s) 890, 1015, 1065
Fnu4HI GCNGC 9 cut(s) 162, 183, 186, 299, 341, 477, 1197, 1200, 1263
FokI GGATG 5 cut(s) 447, 615, 859, 1156, 1265
FriOI GRGCYC 1 cut(s) 294
Fsp4HI GCNGC 9 cut(s) 162, 183, 186, 299, 341, 477, 1197, 1200, 1263
FspBI CTAG 2 cut(s) 242, 272
GlaI GCGC 7 cut(s) 73, 134, 142, 160, 175, 572, 783
GluI GCNGC 9 cut(s) 162, 183, 186, 299, 341, 477, 1197, 1200, 1263
GsuI CTGGAG 4 cut(s) 278, 1068, 1226, 1329
HaeIII GGCC 3 cut(s) 22, 164, 254
HapII CCGG 2 cut(s) 287, 1011
HgaI GACGC 2 cut(s) 124, 457
HhaI GCGC 7 cut(s) 74, 135, 143, 161, 176, 573, 784
Hin1II CATG 8 cut(s) 181, 196, 671, 739, 914, 1100, 1121, 1362
Hin6I GCGC 7 cut(s) 72, 133, 141, 159, 174, 571, 782
HinP1I GCGC 7 cut(s) 72, 133, 141, 159, 174, 571, 782
HincII GTYRAC 2 cut(s) 891, 1312
HindII GTYRAC 2 cut(s) 891, 1312
HinfI GANTC 3 cut(s) 1050, 1297, 1313
HpaII CCGG 2 cut(s) 287, 1011
HphI GGTGA 3 cut(s) 444, 730, 758
Hpy166II GTNNAC 8 cut(s) 204, 749, 891, 974, 1016, 1066, 1123, 1312
Hpy188I TCNGA 5 cut(s) 283, 413, 969, 1156, 1318
Hpy188III TCNNGA 6 cut(s) 383, 651, 661, 776, 1205, 1291
Hpy8I GTNNAC 8 cut(s) 204, 749, 891, 974, 1016, 1066, 1123, 1312
HpyAV CCTTC 2 cut(s) 760, 1142
HpyCH4III ACNGT 4 cut(s) 490, 535, 841, 1225
HpyCH4IV ACGT 2 cut(s) 1165, 1234
HpyF3I CTNAG 5 cut(s) 929, 968, 1042, 1155, 1277
HpySE526I ACGT 2 cut(s) 1165, 1234
Hsp92II CATG 8 cut(s) 181, 196, 671, 739, 914, 1100, 1121, 1362
HspAI GCGC 7 cut(s) 72, 133, 141, 159, 174, 571, 782
Ksp22I TGATCA 1 cut(s) 825
Kzo9I GATC 3 cut(s) 5, 9, 825
LmnI GCTCC 6 cut(s) 238, 289, 297, 348, 919, 1348
Lsp1109I GCAGC 7 cut(s) 194, 310, 352, 463, 1186, 1208, 1274
LweI GCATC 8 cut(s) 558, 593, 793, 881, 1048, 1243, 1249, 1330
MaeI CTAG 2 cut(s) 242, 272
MaeII ACGT 2 cut(s) 1165, 1234
MaeIII GTNAC 4 cut(s) 450, 469, 718, 1219
MalI GATC 3 cut(s) 7, 11, 827
MbiI CCGCTC 1 cut(s) 233
MboI GATC 3 cut(s) 5, 9, 825
MboII GAAGA 7 cut(s) 36, 51, 54, 602, 697, 1306, 1349
MfeI CAATTG 1 cut(s) 939
MhlI GDGCHC 2 cut(s) 294, 976
MluCI AATT 7 cut(s) 126, 354, 672, 810, 831, 858, 939
MlyI GAGTC 3 cut(s) 1059, 1306, 1307
MmeI TCCRAC 3 cut(s) 37, 261, 489
MseI TTAA 5 cut(s) 372, 549, 794, 861, 1137
MslI CAYNNNNRTG 2 cut(s) 101, 740
MspA1I CMGCKG 2 cut(s) 185, 1199
MspI CCGG 2 cut(s) 287, 1011
MssI GTTTAAAC 1 cut(s) 795
MunI CAATTG 1 cut(s) 939
Mva1269I GAATGC 1 cut(s) 407
MvnI CGCG 6 cut(s) 72, 74, 135, 159, 161, 174
NcoI CCATGG 1 cut(s) 177
NdeII GATC 3 cut(s) 5, 9, 825
NlaIII CATG 8 cut(s) 181, 196, 671, 739, 914, 1100, 1121, 1362
NlaIV GGNNCC 3 cut(s) 285, 399, 849
NmuCI GTSAC 3 cut(s) 450, 469, 718
NspI RCATGY 2 cut(s) 914, 1362
PaeI GCATGC 1 cut(s) 914
PctI GAATGC 1 cut(s) 407
PinAI ACCGGT 1 cut(s) 1010
PkrI GCNGC 9 cut(s) 163, 184, 187, 300, 342, 478, 1198, 1201, 1264
PleI GAGTC 3 cut(s) 1058, 1305, 1307
PmeI GTTTAAAC 1 cut(s) 795
PpsI GAGTC 3 cut(s) 1058, 1305, 1307
PshAI GACNNNNGTC 1 cut(s) 315
Psp124BI GAGCTC 1 cut(s) 294
PspEI GGTNACC 1 cut(s) 450
PspN4I GGNNCC 3 cut(s) 285, 399, 849
PspPI GGNCC 3 cut(s) 20, 163, 252
PstNI CAGNNNCTG 1 cut(s) 928
PvuII CAGCTG 1 cut(s) 1199
RsaI GTAC 1 cut(s) 1185
RsaNI GTAC 1 cut(s) 1184
RseI CAYNNNNRTG 2 cut(s) 101, 740
SacI GAGCTC 1 cut(s) 294
SalI GTCGAC 1 cut(s) 889
SaqAI TTAA 5 cut(s) 372, 549, 794, 861, 1137
SatI GCNGC 9 cut(s) 162, 183, 186, 299, 341, 477, 1197, 1200, 1263
Sau3AI GATC 3 cut(s) 5, 9, 825
Sau96I GGNCC 3 cut(s) 20, 163, 252
SchI GAGTC 3 cut(s) 1059, 1306, 1307
SduI GDGCHC 2 cut(s) 294, 976
SfaNI GCATC 8 cut(s) 558, 593, 793, 881, 1048, 1243, 1249, 1330
SfcI CTRYAG 1 cut(s) 328
SmiMI CAYNNNNRTG 2 cut(s) 101, 740
SmlI CTYRAG 2 cut(s) 422, 659
SmoI CTYRAG 2 cut(s) 422, 659
SphI GCATGC 1 cut(s) 914
Sse9I AATT 7 cut(s) 126, 354, 672, 810, 831, 858, 939
SsiI CCGC 7 cut(s) 161, 166, 185, 231, 770, 1061, 1181
SspMI CTAG 2 cut(s) 242, 272
SstI GAGCTC 1 cut(s) 294
StyI CCWWGG 2 cut(s) 177, 271
TaaI ACNGT 4 cut(s) 490, 535, 841, 1225
TaiI ACGT 2 cut(s) 1168, 1237
TaqI TCGA 3 cut(s) 8, 309, 890
TasI AATT 7 cut(s) 126, 354, 672, 810, 831, 858, 939
TauI GCSGC 2 cut(s) 164, 188
Tru1I TTAA 5 cut(s) 372, 549, 794, 861, 1137
Tru9I TTAA 5 cut(s) 372, 549, 794, 861, 1137
TscAI CASTG 5 cut(s) 478, 493, 794, 981, 1009
TseFI GTSAC 3 cut(s) 450, 469, 718
TseI GCWGC 7 cut(s) 182, 298, 340, 476, 1196, 1199, 1262
Tsp45I GTSAC 3 cut(s) 450, 469, 718
TspDTI ATGAA 7 cut(s) 45, 54, 209, 684, 724, 773, 1350
TspRI CASTG 5 cut(s) 478, 493, 794, 981, 1009
VneI GTGCAC 1 cut(s) 972
XceI RCATGY 2 cut(s) 914, 1362
XmaJI CCTAGG 1 cut(s) 271
XmiI GTMKAC 3 cut(s) 890, 1015, 1065
XspI CTAG 2 cut(s) 242, 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.