pycom07g20400

Sorting and assembly machinery

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0009225
Physical Location & Seq
Reverse (-)
22509892 .. 22513070
3179 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1272 bp
ATGCAAGAGCTGCTCGAGGCCGCCGCAATTGCAAATGCGAAGCTTCAGCAGCTTGAAATATTCGATGCGGTTCGAATCACGCTCGATTCTGGCCCGCCGGAGCTGCCTGGAACTACCAATGTTATTGTGGAGGTCGTCGAAACTAAGAGTCCGATTTCTGGCGAAATCGGTGCTTACACGAAGCCGGCGGCTAGGTCATGGACTGCTGAAGGAACACTTAAGTTCAAAAATCTGCTCGGATATGGGGATTTGTGGGATAGTTCTTTGGCATATGGGCCCAACCAAACGTCGGAGTTAAGTGCTGGTCTGTTTTTGCCCAGGTTCAAAGGGTTTGTAACTCCAGTGACGGCACGAGCATTCCTGCTTTCCCAAGATTGGGTGGAATTCTCTTCTTATAAAGAGCGCATGATGGGGCTCTCTCTTGGACTGATTTCATCAAAACACCATGATTTGGCATACAATCTGGGTTGGCGAACCCTAGCAGACCCATCACAATTGGCCTCAAGGTCAATAAGAAGGCAGCTCGGCCATGGATTACTTTCTTCAATCAAATACACATTTAAGAATGATAGGAGAAATTCACCTTTGAGGCCAACTCGAGGTTACTCATTTGTTTCAACGAGTCATATTGGTGGCATTGCACCTGATCATCGAAGCACGAGGTTTTTGCGTCAGGAGTTCGATCTTCGTTATGCGGTTCCATTAGGATTTTATCATGCTGCACTTAATTTTGGGATCTCCGCAGGAGTTATTTTTCCATGGGGGAGTGGATTCTTGAACAAGCCCTCATCCCTTCCAGAGCGGTTCTTCCTGGGTGGTGATTTCTCTCCAGTTTGCACTGTTGGGGGCCCTACGACAGTGTGGGGATTCAAGACAAGGGGACTAGGCCCCACTGAACCAAGAAGGCATGTCGGAGATAATTCTAATGAGGAGAATTCAGAATCCCCTGGAAGAGATTCTGTTGGCGGAGATCTTGCTGTTTCTGCGTTTGCAGACATTTCATTTGATCTTCCACTTCGCTGGTTAAAACAACATGGAGTGCATGGTCATATTTTTGCAGGTGCAGGGAATCTGGCGAAATTGACAGAGAACGAATTCCGAAACTTTTCTGCCCCGAAGTTCCTTCAGTCATTTCGAAGTTCTGTGGGAGCAGGGATTGTTGTCCCTACCAAATTCTTCCGCCTAGAGTGCAACTACTACTATATACTGAAGCAATTTGAGCATGACCGTGGGAAGACTGGCTTTCGCTTCAGCATCTCAGCTCCGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006996 GO:0007005 GO:0007006 GO:0007007 GO:0008150 GO:0009987 GO:0016043 GO:0042407 GO:0061024 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bac_surface_Ag POTRA

Protein Analysis

424

Amino Acids

46.64

Weight (kDa)

9.36

Isoelectric Point (pI)

48.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Omp85 PF01103 77 - 420 3.9e-31 Omp85 superfamily domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 396
AarI CACCTGC 1 cut(s) 1049
Acc36I ACCTGC 1 cut(s) 1049
AccB7I CCANNNNNTGG 1 cut(s) 451
AccBSI CCGCTC 1 cut(s) 802
AclWI GGATC 1 cut(s) 743
AcoI YGGCCR 1 cut(s) 526
AcsI RAATTY 5 cut(s) 383, 577, 934, 1094, 1172
AcuI CTGAAG 5 cut(s) 29, 228, 1109, 1229, 1234
AfiI CCNNNNNNNGG 6 cut(s) 158, 289, 375, 376, 451, 599
AflII CTTAAG 1 cut(s) 218
AgsI TTSAA 7 cut(s) 56, 226, 325, 546, 618, 778, 871
AjnI CCWGG 4 cut(s) 106, 317, 810, 946
AluBI AGCT 6 cut(s) 10, 43, 52, 103, 523, 1262
AluI AGCT 6 cut(s) 10, 43, 52, 103, 523, 1262
AlwI GGATC 1 cut(s) 743
Ama87I CYCGRG 2 cut(s) 14, 597
AoxI GGCC 8 cut(s) 18, 91, 275, 498, 526, 590, 847, 886
ApaI GGGCCC 2 cut(s) 279, 851
ApeKI GCWGC 5 cut(s) 10, 49, 103, 520, 719
ApoI RAATTY 5 cut(s) 383, 577, 934, 1094, 1172
ArsI GACNNNNNNTTYG 2 cut(s) 986, 1018
Asp700I GAANNNNTTC 3 cut(s) 955, 1094, 1105
AspLEI GCGC 1 cut(s) 405
AspS9I GGNCC 6 cut(s) 92, 275, 276, 847, 848, 887
AsuHPI GGTGA 2 cut(s) 573, 830
AsuII TTCGAA 2 cut(s) 73, 1135
AvaI CYCGRG 2 cut(s) 14, 597
BaeGI GKGCMC 2 cut(s) 279, 851
BanII GRGCYC 3 cut(s) 279, 417, 851
BauI CACGAG 2 cut(s) 351, 658
BbsI GAAGAC 1 cut(s) 1241
BbvI GCAGC 4 cut(s) 61, 90, 532, 706
BccI CCATC 2 cut(s) 403, 496
BceAI ACGGC 1 cut(s) 363
BcgI CGANNNNNNTGC 4 cut(s) 152, 186, 649, 683
BciT130I CCWGG 4 cut(s) 108, 319, 812, 948
BclI TGATCA 1 cut(s) 646
BfaI CTAG 4 cut(s) 192, 479, 884, 1184
BfrI CTTAAG 1 cut(s) 218
BfuAI ACCTGC 1 cut(s) 1049
BglII AGATCT 1 cut(s) 970
BisI GCNGC 8 cut(s) 11, 21, 24, 50, 104, 189, 521, 720
BlsI GCNGC 8 cut(s) 12, 22, 25, 51, 105, 190, 522, 721
Bme1390I CCNGG 4 cut(s) 108, 319, 812, 948
BmeT110I CYCGRG 2 cut(s) 14, 597
BmgT120I GGNCC 6 cut(s) 92, 275, 276, 847, 848, 887
BmiI GGNNCC 5 cut(s) 277, 699, 848, 849, 889
BmrFI CCNGG 4 cut(s) 108, 319, 812, 948
BmsI GCATC 2 cut(s) 55, 1263
BpiI GAAGAC 1 cut(s) 1241
BplI GAGNNNNNCTC 2 cut(s) 580, 612
BpmI CTGGAG 2 cut(s) 324, 813
Bpu14I TTCGAA 2 cut(s) 73, 1135
BpuEI CTTGAG 1 cut(s) 487
BsaJI CCNNGG 6 cut(s) 317, 529, 758, 811, 946, 1228
Bsc4I CCNNNNNNNGG 6 cut(s) 158, 289, 375, 376, 451, 599
Bse118I RCCGGY 1 cut(s) 184
Bse1I ACTGG 3 cut(s) 341, 830, 1243
Bse3DI GCAATG 1 cut(s) 636
BseBI CCWGG 4 cut(s) 108, 319, 812, 948
BseDI CCNNGG 6 cut(s) 317, 529, 758, 811, 946, 1228
BseGI GGATG 1 cut(s) 788
BseLI CCNNNNNNNGG 6 cut(s) 158, 289, 375, 376, 451, 599
BseMI GCAATG 1 cut(s) 636
BseMII CTCAG 1 cut(s) 1272
BseNI ACTGG 3 cut(s) 341, 830, 1243
BseRI GAGGAG 1 cut(s) 944
BseSI GKGCMC 2 cut(s) 279, 851
BseXI GCAGC 4 cut(s) 61, 90, 532, 706
BsgI GTGCAG 2 cut(s) 705, 1083
BshFI GGCC 8 cut(s) 20, 93, 277, 500, 528, 592, 849, 888
BsiHKCI CYCGRG 2 cut(s) 14, 597
BsiSI CCGG 2 cut(s) 98, 185
BslFI GGGAC 2 cut(s) 894, 1148
BslI CCNNNNNNNGG 6 cut(s) 158, 289, 375, 376, 451, 599
BsmFI GGGAC 2 cut(s) 894, 1148
BsmI GAATGC 1 cut(s) 356
BsnI GGCC 8 cut(s) 20, 93, 277, 500, 528, 592, 849, 888
BsoBI CYCGRG 2 cut(s) 14, 597
Bsp119I TTCGAA 2 cut(s) 73, 1135
Bsp120I GGGCCC 2 cut(s) 275, 847
Bsp1286I GDGCHC 3 cut(s) 279, 417, 851
Bsp143I GATC 5 cut(s) 646, 682, 735, 970, 1006
Bsp19I CCATGG 2 cut(s) 529, 758
BspANI GGCC 8 cut(s) 20, 93, 277, 500, 528, 592, 849, 888
BspCNI CTCAG 1 cut(s) 1271
BspLI GGNNCC 5 cut(s) 277, 699, 848, 849, 889
BspMI ACCTGC 1 cut(s) 1049
BspPI GGATC 1 cut(s) 743
BspT104I TTCGAA 2 cut(s) 73, 1135
BspTI CTTAAG 1 cut(s) 218
BsrBI CCGCTC 1 cut(s) 802
BsrDI GCAATG 1 cut(s) 636
BsrFI RCCGGY 1 cut(s) 184
BsrI ACTGG 3 cut(s) 341, 830, 1243
BssAI RCCGGY 1 cut(s) 184
BssECI CCNNGG 6 cut(s) 317, 529, 758, 811, 946, 1228
BssMI GATC 5 cut(s) 646, 682, 735, 970, 1006
BssSI CACGAG 2 cut(s) 351, 658
BssT1I CCWWGG 2 cut(s) 529, 758
Bst2BI CACGAG 2 cut(s) 351, 658
Bst2UI CCWGG 4 cut(s) 108, 319, 812, 948
Bst4CI ACNGT 3 cut(s) 841, 859, 1229
Bst6I CTCTTC 2 cut(s) 394, 946
BstAFI CTTAAG 1 cut(s) 218
BstAPI GCANNNNNTGC 1 cut(s) 10
BstBI TTCGAA 2 cut(s) 73, 1135
BstC8I GCNNGC 2 cut(s) 95, 186
BstDEI CTNAG 2 cut(s) 144, 1258
BstDSI CCRYGG 3 cut(s) 529, 758, 1228
BstF5I GGATG 1 cut(s) 788
BstHHI GCGC 1 cut(s) 405
BstKTI GATC 5 cut(s) 649, 685, 738, 973, 1009
BstMBI GATC 5 cut(s) 646, 682, 735, 970, 1006
BstMWI GCNNNNNNNGC 7 cut(s) 10, 29, 49, 103, 983, 1188, 1219
BstNI CCWGG 4 cut(s) 108, 319, 812, 948
BstNSI RCATGY 1 cut(s) 911
BstSCI CCNGG 4 cut(s) 106, 317, 810, 946
BstSLI GKGCMC 2 cut(s) 279, 851
BstV1I GCAGC 4 cut(s) 61, 90, 532, 706
BstV2I GAAGAC 1 cut(s) 1241
BstX2I RGATCY 2 cut(s) 735, 970
BstXI CCANNNNNNTGG 1 cut(s) 1020
BstYI RGATCY 2 cut(s) 735, 970
BsuRI GGCC 8 cut(s) 20, 93, 277, 500, 528, 592, 849, 888
BtgI CCRYGG 3 cut(s) 529, 758, 1228
BtsCI GGATG 1 cut(s) 788
BtsIMutI CAGTG 4 cut(s) 348, 837, 864, 891
BveI ACCTGC 1 cut(s) 1049
Cac8I GCNNGC 2 cut(s) 95, 186
CfoI GCGC 1 cut(s) 405
Cfr10I RCCGGY 1 cut(s) 184
Cfr13I GGNCC 6 cut(s) 92, 275, 276, 847, 848, 887
CseI GACGC 1 cut(s) 659
DdeI CTNAG 2 cut(s) 144, 1258
DpnI GATC 5 cut(s) 648, 684, 737, 972, 1008
DpnII GATC 5 cut(s) 646, 682, 735, 970, 1006
EaeI YGGCCR 1 cut(s) 526
Eam1104I CTCTTC 2 cut(s) 394, 946
EarI CTCTTC 2 cut(s) 394, 946
EciI GGCGGA 2 cut(s) 981, 1169
Eco130I CCWWGG 2 cut(s) 529, 758
Eco24I GRGCYC 3 cut(s) 279, 417, 851
Eco57I CTGAAG 5 cut(s) 29, 228, 1109, 1229, 1234
Eco88I CYCGRG 2 cut(s) 14, 597
EcoO109I RGGNCCY 3 cut(s) 847, 848, 887
EcoRI GAATTC 3 cut(s) 383, 934, 1094
EcoRII CCWGG 4 cut(s) 106, 317, 810, 946
EcoT14I CCWWGG 2 cut(s) 529, 758
EcoT38I GRGCYC 3 cut(s) 279, 417, 851
ErhI CCWWGG 2 cut(s) 529, 758
FalI AAGNNNNNCTT 4 cut(s) 201, 233, 1226, 1258
FaqI GGGAC 2 cut(s) 894, 1148
FauI CCCGC 1 cut(s) 102
FauNDI CATATG 1 cut(s) 271
FbaI TGATCA 1 cut(s) 646
Fnu4HI GCNGC 8 cut(s) 11, 21, 24, 50, 104, 189, 521, 720
FokI GGATG 1 cut(s) 775
FriOI GRGCYC 3 cut(s) 279, 417, 851
Fsp4HI GCNGC 8 cut(s) 11, 21, 24, 50, 104, 189, 521, 720
FspBI CTAG 4 cut(s) 192, 479, 884, 1184
GlaI GCGC 1 cut(s) 404
GluI GCNGC 8 cut(s) 11, 21, 24, 50, 104, 189, 521, 720
GsuI CTGGAG 2 cut(s) 324, 813
HaeIII GGCC 8 cut(s) 20, 93, 277, 500, 528, 592, 849, 888
HapII CCGG 2 cut(s) 98, 185
HgaI GACGC 1 cut(s) 659
HhaI GCGC 1 cut(s) 405
Hin6I GCGC 1 cut(s) 403
HinP1I GCGC 1 cut(s) 403
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 9 cut(s) 75, 86, 148, 622, 771, 867, 941, 956, 1069
HpaII CCGG 2 cut(s) 98, 185
HphI GGTGA 2 cut(s) 573, 830
Hpy188I TCNGA 6 cut(s) 153, 239, 292, 914, 940, 1100
Hpy188III TCNNGA 4 cut(s) 674, 775, 797, 871
Hpy99I CGWCG 2 cut(s) 140, 292
HpyAV CCTTC 5 cut(s) 203, 510, 803, 897, 1133
HpyCH4III ACNGT 3 cut(s) 841, 859, 1229
HpyCH4IV ACGT 1 cut(s) 287
HpyF10VI GCNNNNNNNGC 7 cut(s) 10, 29, 49, 103, 983, 1188, 1219
HpyF3I CTNAG 2 cut(s) 144, 1258
HpySE526I ACGT 1 cut(s) 287
HspAI GCGC 1 cut(s) 403
KroI GCCGGC 1 cut(s) 184
KroNI GCCGGC 1 cut(s) 186
Ksp22I TGATCA 1 cut(s) 646
Kzo9I GATC 5 cut(s) 646, 682, 735, 970, 1006
LmnI GCTCC 3 cut(s) 100, 1148, 1267
Lsp1109I GCAGC 4 cut(s) 61, 90, 532, 706
LweI GCATC 2 cut(s) 55, 1263
MaeI CTAG 4 cut(s) 192, 479, 884, 1184
MaeII ACGT 1 cut(s) 287
MaeIII GTNAC 3 cut(s) 334, 343, 602
MalI GATC 5 cut(s) 648, 684, 737, 972, 1008
MbiI CCGCTC 1 cut(s) 802
MboI GATC 5 cut(s) 646, 682, 735, 970, 1006
MboII GAAGA 8 cut(s) 381, 534, 677, 799, 963, 1001, 1168, 1246
MfeI CAATTG 2 cut(s) 27, 494
MflI RGATCY 2 cut(s) 735, 970
MhlI GDGCHC 3 cut(s) 279, 417, 851
MlyI GAGTC 2 cut(s) 157, 631
MmeI TCCRAC 2 cut(s) 270, 892
MnlI CCTC 8 cut(s) 10, 124, 511, 582, 593, 654, 796, 922
MroNI GCCGGC 1 cut(s) 184
MroXI GAANNNNTTC 3 cut(s) 955, 1094, 1105
MseI TTAA 5 cut(s) 219, 296, 561, 726, 1025
MslI CAYNNNNRTG 2 cut(s) 630, 1227
MspCI CTTAAG 1 cut(s) 218
MspI CCGG 2 cut(s) 98, 185
MspR9I CCNGG 4 cut(s) 108, 319, 812, 948
MunI CAATTG 2 cut(s) 27, 494
Mva1269I GAATGC 1 cut(s) 356
MvaI CCWGG 4 cut(s) 108, 319, 812, 948
MwoI GCNNNNNNNGC 7 cut(s) 10, 29, 49, 103, 983, 1188, 1219
NaeI GCCGGC 1 cut(s) 186
NcoI CCATGG 2 cut(s) 529, 758
NdeI CATATG 1 cut(s) 271
NdeII GATC 5 cut(s) 646, 682, 735, 970, 1006
NgoMIV GCCGGC 1 cut(s) 184
NlaIV GGNNCC 5 cut(s) 277, 699, 848, 849, 889
NmeAIII GCCGAG 1 cut(s) 504
NmuCI GTSAC 1 cut(s) 343
NspI RCATGY 1 cut(s) 911
NspV TTCGAA 2 cut(s) 73, 1135
PaeR7I CTCGAG 2 cut(s) 14, 597
PaqCI CACCTGC 1 cut(s) 1049
PctI GAATGC 1 cut(s) 356
PdiI GCCGGC 1 cut(s) 186
PdmI GAANNNNTTC 3 cut(s) 955, 1094, 1105
PfeI GAWTC 7 cut(s) 75, 86, 771, 867, 941, 956, 1069
PflMI CCANNNNNTGG 1 cut(s) 451
PkrI GCNGC 8 cut(s) 12, 22, 25, 51, 105, 190, 522, 721
PleI GAGTC 2 cut(s) 156, 630
PpsI GAGTC 2 cut(s) 156, 630
PsiI TTATAA 1 cut(s) 396
Psp6I CCWGG 4 cut(s) 106, 317, 810, 946
PspGI CCWGG 4 cut(s) 106, 317, 810, 946
PspN4I GGNNCC 5 cut(s) 277, 699, 848, 849, 889
PspOMI GGGCCC 2 cut(s) 275, 847
PspPI GGNCC 6 cut(s) 92, 275, 276, 847, 848, 887
PspXI VCTCGAGB 2 cut(s) 14, 597
PsuI RGATCY 2 cut(s) 735, 970
RseI CAYNNNNRTG 2 cut(s) 630, 1227
SaqAI TTAA 5 cut(s) 219, 296, 561, 726, 1025
SatI GCNGC 8 cut(s) 11, 21, 24, 50, 104, 189, 521, 720
Sau3AI GATC 5 cut(s) 646, 682, 735, 970, 1006
Sau96I GGNCC 6 cut(s) 92, 275, 276, 847, 848, 887
SchI GAGTC 2 cut(s) 157, 631
ScrFI CCNGG 4 cut(s) 108, 319, 812, 948
SduI GDGCHC 3 cut(s) 279, 417, 851
SfaNI GCATC 2 cut(s) 55, 1263
Sfr274I CTCGAG 2 cut(s) 14, 597
SfuI TTCGAA 2 cut(s) 73, 1135
SlaI CTCGAG 2 cut(s) 14, 597
SmiMI CAYNNNNRTG 2 cut(s) 630, 1227
SmlI CTYRAG 4 cut(s) 14, 218, 502, 597
SmoI CTYRAG 4 cut(s) 14, 218, 502, 597
SspI AATATT 1 cut(s) 60
SspMI CTAG 4 cut(s) 192, 479, 884, 1184
StyD4I CCNGG 4 cut(s) 106, 317, 810, 946
StyI CCWWGG 2 cut(s) 529, 758
TaaI ACNGT 3 cut(s) 841, 859, 1229
TaiI ACGT 1 cut(s) 290
TaqI TCGA 9 cut(s) 15, 63, 73, 84, 138, 598, 652, 681, 1135
TauI GCSGC 3 cut(s) 23, 26, 191
TfiI GAWTC 7 cut(s) 75, 86, 771, 867, 941, 956, 1069
Tru1I TTAA 5 cut(s) 219, 296, 561, 726, 1025
Tru9I TTAA 5 cut(s) 219, 296, 561, 726, 1025
TscAI CASTG 4 cut(s) 348, 844, 864, 898
TseFI GTSAC 1 cut(s) 343
TseI GCWGC 5 cut(s) 10, 49, 103, 520, 719
Tsp45I GTSAC 1 cut(s) 343
TspDTI ATGAA 2 cut(s) 423, 990
TspGWI ACGGA 1 cut(s) 1254
TspRI CASTG 4 cut(s) 348, 844, 864, 898
Van91I CCANNNNNTGG 1 cut(s) 451
Vha464I CTTAAG 1 cut(s) 218
XapI RAATTY 5 cut(s) 383, 577, 934, 1094, 1172
XceI RCATGY 1 cut(s) 911
XcmI CCANNNNNNNNNTGG 1 cut(s) 124
XhoI CTCGAG 2 cut(s) 14, 597
XmnI GAANNNNTTC 3 cut(s) 955, 1094, 1105
XspI CTAG 4 cut(s) 192, 479, 884, 1184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.