pycom07g21770

N-terminal cysteine-rich region of Transmembrane protein 135

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0012420
Physical Location & Seq
Forward (+)
23341639 .. 23343699
2061 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1116 bp
ATGAATGCAATTTTAGCAGGTTCAGTTGCTGGTTTGTCTATTTTAGCATTGAATGACTCAAACCGAAGGCGCACACTTTCTCTGTACTTACTGGCCAGGCTAGGCCAGTGTGCTTATAATTCTGCGAAGTCTAAGAATAAGTTTCACTTTTGGGGAAGCCATTGGAAGCATGGAGATTCATTACTTTTCTCTCTTGCATGTGCGCAGGTTATGTATGCCTTTGTAATGCGGCCTGAGAGCTTGCCAAAATCTTATCAAGAATTTATTCAGAAGACTGGGCCAGTTGCCCAACCTGTATACAAGGCTGTAAGGGATGGCTGTAGAGGCCACCCTGTGGATATTGCCTCTCTATCTGATTATTTGTTTGAGAGAACAAAATCCAACTCTATACAGTTGGAAGAGTATCCATCCATACTTCCTTGTACAGTTATTCATCCAGACACGAATTCTTGTTTAGCTCATAATGCACGGGCAACATCAGCTACATTTAGGAAAACATTTCCGCTTTACTTTTCTTTGACGTTTGTACCATTTGTTGTTCTCCGCCTACAGAAGTTCATGGAGGCCCCTTCTCGCACATTTTTAATTGCTCTTAAAGATGCTGTTCGCTCAACATCATTTTTGTCCGCATTTGTTGGAATCTTTCAGGGTGTCATATGTTTGCATAGAAAAGTTGCTTCAAAAGACCACAAGCTTGTATATTGGTTTGCAGGTGCAATATCTGCTCTTTCAGTTTTACTGGAGAAAAAGCCTAGACGTGGTGAACTGGCTCTTTATGTTCTTCCACGAGCTGGAGATTCTTTGTGGTATATCTTAGTAAACCGCCACCTTCTTCCAGATATTAAGAACGCAGAGGTGTTCATGTTCAGTTTGTGCATGGGAGGAATCATGTACTACTTGGAACATGAGCCAGACACAATGGCTCCACTCCTCAGGGGTCTGATCCGTCGGTTCCTTGCTAGCAGAATAAGCAACCCTGTGTCTTCGTCTAGTAGGAATCCTTCCTACACATACTTGCAAAATCTTGAAGCCATGAAGAAACCGAAGCCCTTAGAGAGTCGCGGTGCTGAATCACCTTCCGAAAAATATAATCTCGAATCAATACCAGGGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM135_C_rich Tim17

Protein Analysis

372

Amino Acids

41.7

Weight (kDa)

9.64

Isoelectric Point (pI)

46.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM135_C_rich PF15982 144 - 271 2.6e-09 N-terminal cysteine-rich region of Transmembrane protein 135
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51150
fragaria_vesca FvH4_7g26640 FvH4_7g26640 FvH4_7g26640
malus_domestica MD07G1241200.v1.1
prunus_persica Prupe.2G270300_v2.0.a1
pyrus_communis pycom07g21770
rosa_chinensis RchiOBHm_Chr1g0373261
rosa_laevigata RLG00000026833
rosa_multiflora Rmu_co8510213.1_g000001 Rmu_sc0031315.1_g000001
rosa_roxburghii Rroxscaffold_4G00284300 Rroxscaffold_4G00284340
rosa_rugosa Rorug01G0376800
rosa_samantha Rh1AG385500 Rh1BG349700 Rh1CG362900 Rh1DG380400
rosa_wichuraiana Rw1G034940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 117
AarI CACCTGC 1 cut(s) 701
Acc16I TGCGCA 1 cut(s) 204
Acc36I ACCTGC 3 cut(s) 8, 196, 701
AccB7I CCANNNNNTGG 2 cut(s) 334, 791
AccI GTMKAC 1 cut(s) 297
AccII CGCG 1 cut(s) 1062
AciI CCGC 6 cut(s) 229, 503, 544, 627, 823, 1062
AclWI GGATC 1 cut(s) 937
AcoI YGGCCR 1 cut(s) 93
AcsI RAATTY 2 cut(s) 260, 445
AdeI CACNNNGTG 1 cut(s) 334
AfaI GTAC 4 cut(s) 86, 424, 528, 893
AfiI CCNNNNNNNGG 3 cut(s) 334, 758, 791
AgsI TTSAA 3 cut(s) 52, 681, 1028
AjiI CACGTC 1 cut(s) 758
AjnI CCWGG 2 cut(s) 95, 1105
AluBI AGCT 5 cut(s) 240, 458, 482, 694, 791
AluI AGCT 5 cut(s) 240, 458, 482, 694, 791
AlwI GGATC 1 cut(s) 937
AlwNI CAGNNNCTG 1 cut(s) 29
AoxI GGCC 6 cut(s) 93, 103, 230, 278, 325, 564
ApoI RAATTY 2 cut(s) 260, 445
Asp700I GAANNNNTTC 1 cut(s) 264
AspLEI GCGC 2 cut(s) 72, 205
AspS9I GGNCC 2 cut(s) 278, 565
AsuHPI GGTGA 2 cut(s) 773, 1065
AsuNHI GCTAGC 1 cut(s) 959
AxyI CCTNAGG 1 cut(s) 932
BaeI ACNNNNGTAYC 2 cut(s) 510, 543
BalI TGGCCA 1 cut(s) 95
BanII GRGCYC 1 cut(s) 1113
BarI GAAGNNNNNNTAC 2 cut(s) 985, 1017
BauI CACGAG 1 cut(s) 786
BbsI GAAGAC 2 cut(s) 278, 975
BccI CCATC 2 cut(s) 308, 415
BciT130I CCWGG 2 cut(s) 97, 1107
BciVI GTATCC 1 cut(s) 414
BfaI CTAG 4 cut(s) 101, 753, 960, 990
BfmI CTRYAG 2 cut(s) 319, 548
BfuAI ACCTGC 3 cut(s) 8, 196, 701
BfuI GTATCC 1 cut(s) 414
BisI GCNGC 1 cut(s) 230
BlsI GCNGC 1 cut(s) 231
Bme1390I CCNGG 2 cut(s) 97, 1107
BmgBI CACGTC 1 cut(s) 758
BmgT120I GGNCC 2 cut(s) 278, 565
BmiI GGNNCC 3 cut(s) 567, 924, 953
BmrFI CCNGG 2 cut(s) 97, 1107
BmrI ACTGGG 1 cut(s) 285
BmsI GCATC 1 cut(s) 589
BmtI GCTAGC 1 cut(s) 963
BmuI ACTGGG 1 cut(s) 285
BpiI GAAGAC 2 cut(s) 278, 975
BpmI CTGGAG 2 cut(s) 761, 813
BsaJI CCNNGG 1 cut(s) 1106
BsaXI ACNNNNNCTCC 2 cut(s) 907, 937
Bsc4I CCNNNNNNNGG 3 cut(s) 334, 758, 791
Bse1I ACTGG 6 cut(s) 96, 106, 280, 281, 744, 771
Bse21I CCTNAGG 1 cut(s) 932
BseBI CCWGG 2 cut(s) 97, 1107
BseDI CCNNGG 1 cut(s) 1106
BseGI GGATG 3 cut(s) 319, 407, 433
BseLI CCNNNNNNNGG 3 cut(s) 334, 758, 791
BseMII CTCAG 2 cut(s) 225, 946
BseNI ACTGG 6 cut(s) 96, 106, 280, 281, 744, 771
BseRI GAGGAG 1 cut(s) 920
Bsh1236I CGCG 1 cut(s) 1062
BshFI GGCC 6 cut(s) 95, 105, 232, 280, 327, 566
BslI CCNNNNNNNGG 3 cut(s) 334, 758, 791
BsmI GAATGC 1 cut(s) 10
BsnI GGCC 6 cut(s) 95, 105, 232, 280, 327, 566
Bsp1286I GDGCHC 1 cut(s) 1113
Bsp1407I TGTACA 1 cut(s) 422
Bsp143I GATC 1 cut(s) 942
BspACI CCGC 6 cut(s) 229, 503, 544, 627, 823, 1062
BspANI GGCC 6 cut(s) 95, 105, 232, 280, 327, 566
BspCNI CTCAG 2 cut(s) 226, 945
BspFNI CGCG 1 cut(s) 1062
BspLI GGNNCC 3 cut(s) 567, 924, 953
BspMI ACCTGC 3 cut(s) 8, 196, 701
BspOI GCTAGC 1 cut(s) 963
BspPI GGATC 1 cut(s) 937
BsrGI TGTACA 1 cut(s) 422
BsrI ACTGG 6 cut(s) 96, 106, 280, 281, 744, 771
BssECI CCNNGG 1 cut(s) 1106
BssMI GATC 1 cut(s) 942
BssNAI GTATAC 1 cut(s) 298
BssSI CACGAG 1 cut(s) 786
Bst1107I GTATAC 1 cut(s) 298
Bst2BI CACGAG 1 cut(s) 786
Bst2UI CCWGG 2 cut(s) 97, 1107
Bst4CI ACNGT 2 cut(s) 393, 427
Bst6I CTCTTC 1 cut(s) 393
BstAPI GCANNNNNTGC 1 cut(s) 722
BstAUI TGTACA 1 cut(s) 422
BstC8I GCNNGC 2 cut(s) 242, 961
BstDEI CTNAG 5 cut(s) 132, 234, 814, 932, 1051
BstF5I GGATG 3 cut(s) 319, 407, 433
BstFNI CGCG 1 cut(s) 1062
BstHHI GCGC 2 cut(s) 72, 205
BstKTI GATC 1 cut(s) 945
BstMBI GATC 1 cut(s) 942
BstMWI GCNNNNNNNGC 6 cut(s) 14, 324, 464, 479, 722, 969
BstNI CCWGG 2 cut(s) 97, 1107
BstNSI RCATGY 1 cut(s) 201
BstSCI CCNGG 2 cut(s) 95, 1105
BstSFI CTRYAG 2 cut(s) 319, 548
BstUI CGCG 1 cut(s) 1062
BstV2I GAAGAC 2 cut(s) 278, 975
BstZ17I GTATAC 1 cut(s) 298
Bsu36I CCTNAGG 1 cut(s) 932
BsuI GTATCC 1 cut(s) 414
BsuRI GGCC 6 cut(s) 95, 105, 232, 280, 327, 566
BtrI CACGTC 1 cut(s) 758
BtsCI GGATG 3 cut(s) 319, 407, 433
BtsIMutI CAGTG 1 cut(s) 113
BveI ACCTGC 3 cut(s) 8, 196, 701
Cac8I GCNNGC 2 cut(s) 242, 961
CaiI CAGNNNCTG 1 cut(s) 29
CfoI GCGC 2 cut(s) 72, 205
Cfr13I GGNCC 2 cut(s) 278, 565
Csp6I GTAC 4 cut(s) 85, 423, 527, 892
CviAII CATG 8 cut(s) 170, 198, 559, 862, 877, 889, 905, 1033
CviQI GTAC 4 cut(s) 85, 423, 527, 892
DdeI CTNAG 5 cut(s) 132, 234, 814, 932, 1051
DpnI GATC 1 cut(s) 944
DpnII GATC 1 cut(s) 942
DraIII CACNNNGTG 1 cut(s) 334
EaeI YGGCCR 1 cut(s) 93
Eam1104I CTCTTC 1 cut(s) 393
EarI CTCTTC 1 cut(s) 393
EciI GGCGGA 1 cut(s) 533
Eco24I GRGCYC 1 cut(s) 1113
Eco81I CCTNAGG 1 cut(s) 932
EcoO109I RGGNCCY 1 cut(s) 565
EcoRI GAATTC 1 cut(s) 445
EcoRII CCWGG 2 cut(s) 95, 1105
EcoT38I GRGCYC 1 cut(s) 1113
FaeI CATG 8 cut(s) 173, 201, 562, 865, 880, 892, 908, 1036
FalI AAGNNNNNCTT 2 cut(s) 131, 163
FatI CATG 8 cut(s) 169, 197, 558, 861, 876, 888, 904, 1032
FauNDI CATATG 1 cut(s) 656
FblI GTMKAC 1 cut(s) 297
Fnu4HI GCNGC 1 cut(s) 230
FokI GGATG 3 cut(s) 326, 394, 420
FriOI GRGCYC 1 cut(s) 1113
Fsp4HI GCNGC 1 cut(s) 230
FspBI CTAG 4 cut(s) 101, 753, 960, 990
FspI TGCGCA 1 cut(s) 204
GlaI GCGC 2 cut(s) 71, 204
GluI GCNGC 1 cut(s) 230
GsuI CTGGAG 2 cut(s) 761, 813
HaeIII GGCC 6 cut(s) 95, 105, 232, 280, 327, 566
HhaI GCGC 2 cut(s) 72, 205
Hin1II CATG 8 cut(s) 173, 201, 562, 865, 880, 892, 908, 1036
Hin6I GCGC 2 cut(s) 70, 203
HinP1I GCGC 2 cut(s) 70, 203
HindIII AAGCTT 1 cut(s) 692
HinfI GANTC 9 cut(s) 56, 176, 639, 797, 885, 997, 1057, 1070, 1097
HphI GGTGA 2 cut(s) 773, 1065
Hpy166II GTNNAC 3 cut(s) 298, 764, 820
Hpy188I TCNGA 5 cut(s) 270, 355, 942, 1081, 1115
Hpy188III TCNNGA 5 cut(s) 257, 437, 836, 1025, 1094
Hpy8I GTNNAC 3 cut(s) 298, 764, 820
Hpy99I CGWCG 1 cut(s) 951
HpyAV CCTTC 5 cut(s) 60, 579, 839, 1011, 1086
HpyCH4III ACNGT 2 cut(s) 393, 427
HpyCH4IV ACGT 2 cut(s) 521, 757
HpyCH4V TGCA 8 cut(s) 8, 197, 467, 664, 710, 716, 876, 1018
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 324, 464, 479, 722, 969
HpyF3I CTNAG 5 cut(s) 132, 234, 814, 932, 1051
HpySE526I ACGT 2 cut(s) 521, 757
Hsp92II CATG 8 cut(s) 173, 201, 562, 865, 880, 892, 908, 1036
HspAI GCGC 2 cut(s) 70, 203
Kzo9I GATC 1 cut(s) 942
LmnI GCTCC 1 cut(s) 928
LweI GCATC 1 cut(s) 589
MaeI CTAG 4 cut(s) 101, 753, 960, 990
MaeII ACGT 2 cut(s) 521, 757
MalI GATC 1 cut(s) 944
MboI GATC 1 cut(s) 942
MboII GAAGA 6 cut(s) 283, 410, 773, 824, 975, 1048
MhlI GDGCHC 1 cut(s) 1113
MlsI TGGCCA 1 cut(s) 95
MluCI AATT 5 cut(s) 9, 118, 260, 445, 585
MluNI TGGCCA 1 cut(s) 95
MlyI GAGTC 2 cut(s) 50, 1066
MmeI TCCRAC 3 cut(s) 375, 405, 616
MnlI CCTC 6 cut(s) 317, 355, 556, 847, 875, 941
Mox20I TGGCCA 1 cut(s) 95
MroXI GAANNNNTTC 1 cut(s) 264
MscI TGGCCA 1 cut(s) 95
MseI TTAA 3 cut(s) 584, 594, 843
Msp20I TGGCCA 1 cut(s) 95
MspR9I CCNGG 2 cut(s) 97, 1107
Mva1269I GAATGC 1 cut(s) 10
MvaI CCWGG 2 cut(s) 97, 1107
MvnI CGCG 1 cut(s) 1062
MwoI GCNNNNNNNGC 6 cut(s) 14, 324, 464, 479, 722, 969
NdeI CATATG 1 cut(s) 656
NdeII GATC 1 cut(s) 942
NheI GCTAGC 1 cut(s) 959
NlaIII CATG 8 cut(s) 173, 201, 562, 865, 880, 892, 908, 1036
NlaIV GGNNCC 3 cut(s) 567, 924, 953
NsbI TGCGCA 1 cut(s) 204
NspI RCATGY 1 cut(s) 201
PaqCI CACCTGC 1 cut(s) 701
PctI GAATGC 1 cut(s) 10
PdmI GAANNNNTTC 1 cut(s) 264
PfeI GAWTC 7 cut(s) 176, 639, 797, 885, 997, 1070, 1097
PflMI CCANNNNNTGG 2 cut(s) 334, 791
PkrI GCNGC 1 cut(s) 231
PleI GAGTC 2 cut(s) 50, 1065
PpsI GAGTC 2 cut(s) 50, 1065
PsiI TTATAA 1 cut(s) 117
Psp6I CCWGG 2 cut(s) 95, 1105
PspGI CCWGG 2 cut(s) 95, 1105
PspN4I GGNNCC 3 cut(s) 567, 924, 953
PspPI GGNCC 2 cut(s) 278, 565
PstNI CAGNNNCTG 1 cut(s) 29
RsaI GTAC 4 cut(s) 86, 424, 528, 893
RsaNI GTAC 4 cut(s) 85, 423, 527, 892
SaqAI TTAA 3 cut(s) 584, 594, 843
SatI GCNGC 1 cut(s) 230
Sau3AI GATC 1 cut(s) 942
Sau96I GGNCC 2 cut(s) 278, 565
SchI GAGTC 2 cut(s) 50, 1066
ScrFI CCNGG 2 cut(s) 97, 1107
SduI GDGCHC 1 cut(s) 1113
SfaNI GCATC 1 cut(s) 589
SfcI CTRYAG 2 cut(s) 319, 548
Sse9I AATT 5 cut(s) 9, 118, 260, 445, 585
SsiI CCGC 6 cut(s) 229, 503, 544, 627, 823, 1062
SspMI CTAG 4 cut(s) 101, 753, 960, 990
StyD4I CCNGG 2 cut(s) 95, 1105
TaaI ACNGT 2 cut(s) 393, 427
TaiI ACGT 2 cut(s) 524, 760
TaqI TCGA 1 cut(s) 1095
TasI AATT 5 cut(s) 9, 118, 260, 445, 585
TatI WGTACW 3 cut(s) 84, 422, 891
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 7 cut(s) 176, 639, 797, 885, 997, 1070, 1097
Tru1I TTAA 3 cut(s) 584, 594, 843
Tru9I TTAA 3 cut(s) 584, 594, 843
TscAI CASTG 1 cut(s) 113
TspDTI ATGAA 6 cut(s) 17, 168, 422, 547, 850, 1049
TspGWI ACGGA 1 cut(s) 935
TspRI CASTG 1 cut(s) 113
Van91I CCANNNNNTGG 2 cut(s) 334, 791
XapI RAATTY 2 cut(s) 260, 445
XceI RCATGY 1 cut(s) 201
XcmI CCANNNNNNNNNTGG 1 cut(s) 167
XmiI GTMKAC 1 cut(s) 297
XmnI GAANNNNTTC 1 cut(s) 264
XspI CTAG 4 cut(s) 101, 753, 960, 990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.