Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0012250

Physical Location & Seq

Genomic Coordinates Forward (+)

23775654 .. 23779866

Gene Structure 4213 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3102 bp

ATGAAAGTTCCTTCCTTTTCCTCCTCCTTCCCTCTCTCTCTCATCCTCTTCTTCTCTCTCCAGACCTCCATAACTCTCTGCTCTTCTTCCTCTTCTTCTTCTCTCTCTTTCCAGCTCATTGCTCTGCTCTCTCTCAAGTCCTCCCTCAAAGACCCTTTCTCCTCCTTCCACGACTGGGTTCTCCCCAATTTCCAACAAAACGGCGGCGTCTCCGGTTGGTGTTCGTGGTCCGGCGTTAAATGCCACCCCAACACCTCCCAAGTCGTCGCCCTCGACCTCTCACGCCGCAACCTCTCCGGCCAAATCCCACCTCAAATCCGCCAACTTTCTTCCCTAATCTACCTCAATTTGAGCGGCAACGCCTTCGACGGCCCTCTCCCTCCCGCCATTTTCGAGCTCGGCAACTTAAGGATCCTCGACATCAGCCACAACGACTTTAAGTCGGATTTCCCGCCTGGGATTTCAAAGCTCAAGTTTTTAAGAATCTTCACCGCTTACAGCAACAGCTTCACCGGCCCATTGCCTCGGGAATTCATCAGACTCCGGTATCTCGAGCGGCTCAACCTTGGCGGAAGCTACTTCAACGGCGAAATCCCGGAAGGGTACGGAAGTTTCCCGAGATTGAAATTCCTTTACCTCGCCGGAAATGCACTTAACGGTTCAATTCCACCGCAATTGGGCTTCTTATCGGAGCTGACCCGGATGGAGATCGGGTACAACGAGTATTCAGGTGGGTTTCCTGCACAACTCGCAATGCTGTCAAATCTCACGTACTTGGACGTCTCCAACGCCTTTCTCTCCGGCGCTCTCCCGCCGGAGCTCGGCAACTTAACCATGCTCGACACATTGCTGCTCTTCAAGAACCGATTTTCGGGATTTATTCCGGCAACTCTAGGCCGCTTACAGGGTCTGAAGACTTTCGATTTGTCGGATAATCAGCTCTCCGGCTCAATCCCACCGGAAATTGTAACGTTGAAAGGGCTGACCAATATAAGCCTGATGAACAACTTTCTCGTCGGAGAAATCCCGGAAAAAATGGGAGAGCTGCCCAACTTGGAAAACCTCCTCCTCTGGAACAATTCACTGAAGGGTGTCCTCCCCCAGAGCTTAGGATTCAGTGGGAAGCTCCTGCGAGTCGACGTTTCCTCCAATTCTCTCACCGGACCGATCCCGCCAAATCTCTGCAATGGCAACAAGCTCCAAAAGCTCATACTTTTCTCCAACAGGTTCACCGACCCACTTCCCAAAACGTTAGCAAACTGCAATTCTCTTTTACGACTTCGAATCCAGGATAACCAAATCAATGGGTCGGTTCCGACGGGTTTCGGGTTGTTACCCAATTTGACCTACGTCGATTTGAGTGGCAACAATTTCACCGGCGCGATACCTGAAGATCTCGGCAATGCCGAAGAGCTCAGTTACTTGAACATTTCGCTGAACCCACTTCACTCAGTCCTTCCGTCGAACATCTGGCGTGCACCAAATCTCCAGATCTTCTCCGCCAGTTCCGGCCACCTCACCGGTAAAATCCCCGACTTCATCGGCTGCAAAAGCCTGTACAAGATTGAACTCCAACGTAACGACTTTAACGGCTCGATCCCGTGGGACATCGGGCACTGCGAGAAGCTTCTCAGCTTGAATCTGAGCCGCAATTCTCTCACCGGCATTATCCCATGGGAAATCTCCGCTTTGCCCTCCATTACCGACCTCGACCTCTCCCGTAATTTTCTCTCCGGTACGATTCCGTCCAACTTCGAGAACTGTAGCACGCTGGAGAGCTTCAACGTTTCGTTTAATTTGTTGACCGGTCCGATCCCGTCCGGTTCGATTTTCCTGAATCTCCATCCGACGTCGTTTTTGGGGAATGAAGGATTATGTGGCGGCGCTCTGGGGAAGCCCTGTGACACGTTGTCAGCCGGAGCCGTGGACGTTCGCGGCCACGAGGAGCCGAAGAAGACGGCGGGCGCGATAGTATGGATTATGGCGGCTGCTTTCGGTATCGGGCTTTTTGTTCTCGTCGCTGGGACCCGCTGCTTCCACGCGAATTACAGCCGTCAGATGGACGAGAGTCAGCAGATCGGACCGTGGAAATTAACCGCTTTCCAGCGGTTAAATTTCACGGCGGATGACGTGTTGGAGTGCCTGGAGATGAGTAATAAGATTATAGGGATGGGGTCCACGGGGACGGTTTATAGAGCTGAGATGCCAGGTGGCGAGATCATAGCGGTGAAGAAACTGTGGGGGAAACAGAAGGAGAACAGTAGTATTGTGATCCGACGGCGAAGAGGAGTTCTGGCTGAAGTGGAGGTGTTGGGGAACGTTAGGCACAGGAACATTGTGAGATTGTTAGGATGCTGCTGCAACAGGGACATCACCATGCTGCTCTACGAGTACATGCCCAATGGCAATTTAGATGATCTGTTGCATGGCAAAAACAAGGCTCAGAATTTGGGGGCGGATTGGGTCACGAGATACAAGATCGCCCTCGGCGTGGCGCAGGGAATTTGCTACCTGCACCACGACTGTGATCCGGTGATTGTGCACCGTGATCTTAAGCCAAGTAATATCTTATTGGATAGTGAAATGGAGGCTAGGGTGGCAGATTTCGGGGTGGCCAAGTTGATCCAGAGCGATGAGTCCATGTCTGTCATCGCCGGCTCCTATGGCTACATTGCACCAGAATATGCTTATACCCTACAAGTAGATGAAAAGAGTGATATATACAGCTATGGGGTGGTGCTGATGGAGATCTTAAGCGGGAAAAGATCGGTCGACGCCGAGTTTGGAGACGGGAATAGCATTGTGCATTGGGTTAGGTCGAAGATCAAGATGAAAGAAGCGATCAATGACGTTTTGGACAAGAATGCAGGGGCGGGTTGCGAGCCGGTGAGGGAGGAGATGATGCAGATGCTTAGGATTGCTTTGCTCTGCACGAGCCAGAACCCCGCGGACCGGCCGTCGATGAGGGACGTGGTGTTGATGCTGCTCGAGGCCAAACCCAAGAGGAAGTTGCTAGCTAGTGTTGGGCATGGCCACAATGGGAGTGGTTGTGCTGAGTATCATGATATTCCTTTGCCACAAAAGCCAGCGGCGGAATGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001763 GO:0003006 GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010016 GO:0010065 GO:0010067 GO:0010087 GO:0010089 GO:0010154 GO:0010223 GO:0010346 GO:0016020 GO:0022414 GO:0032501 GO:0032502 GO:0044464 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0051301 GO:0061458 GO:0071944 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

1034

Amino Acids

113.18

Weight (kDa)

6.55

Isoelectric Point (pI)

34.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	273 - 478	6.1e-09	Leucine-rich repeat region
LRR_8	PF13855	399 - 458	8.4e-07	Leucine rich repeat
LRR_8	PF13855	518 - 575	5.9e-07	Leucine rich repeat
Pkinase	PF00069	719 - 992	5.3e-46	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	719 - 993	2.6e-40	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0012250)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G61480
fragaria_vesca	FvH4_7g27270
malus_domestica	MD01G1181700.v1.1 MD07G1251900.v1.1
prunus_persica	Prupe.2G275500_v2.0.a1
pyrus_communis	pycom01g19150 pycom07g22430
rosa_chinensis	RchiOBHm_Chr1g0374101
rosa_laevigata	RLG00000026763
rosa_multiflora	Rmu_co8312173.1_g000001 Rmu_co8440105.1_g000001 Rmu_sc0006924.1_g000013
rosa_roxburghii	Rroxscaffold_4G00283740
rosa_rugosa	Rorug01G0380800
rosa_samantha	Rh1AG391500 Rh1BG355500 Rh1CG368500 Rh1DG386200
rosa_wichuraiana	Rw1G034410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	783, 1853
Acc36I	ACCTGC	1 cut(s)	2520
AccBSI	CCGCTC	2 cut(s)	354, 556
AccI	GTMKAC	2 cut(s)	1137, 2772
AccII	CGCG	5 cut(s)	1382, 1935, 1967, 2042, 2948
AclI	AACGTT	4 cut(s)	971, 1250, 1785, 2319
AclWI	GGATC	8 cut(s)	406, 419, 1162, 1591, 1807, 2266, 2522, 2617
AcoI	YGGCCR	6 cut(s)	298, 1510, 1936, 2613, 2954, 3031
AcsI	RAATTY	5 cut(s)	530, 626, 2113, 2446, 2502
AcuI	CTGAAG	4 cut(s)	932, 1106, 1410, 2319
AcyI	GRCGYC	4 cut(s)	207, 780, 1850, 2775
AfaI	GTAC	6 cut(s)	605, 716, 773, 1559, 1738, 2393
AfiI	CCNNNNNNNGG	8 cut(s)	175, 677, 821, 871, 904, 2059, 2491, 2952
AflII	CTTAAG	3 cut(s)	406, 2552, 2752
AflIII	ACRYGT	2 cut(s)	1905, 2130
AgeI	ACCGGT	2 cut(s)	1520, 1805
AhdI	GACNNNNNGTC	3 cut(s)	439, 1909, 2956
AjiI	CACGTC	2 cut(s)	2131, 2971
AjnI	CCWGG	4 cut(s)	454, 1287, 2142, 2206
AleI	CACNNNNGTG	1 cut(s)	2523
Alw21I	GWGCWC	5 cut(s)	399, 822, 1416, 1480, 2544
Alw26I	GTCTC	3 cut(s)	214, 787, 2781
Alw44I	GTGCAC	2 cut(s)	1476, 2540
AlwI	GGATC	8 cut(s)	406, 419, 1162, 1591, 1807, 2266, 2522, 2617
AlwNI	CAGNNNCTG	1 cut(s)	910
Ama87I	CYCGRG	4 cut(s)	525, 551, 616, 2987
ApaLI	GTGCAC	2 cut(s)	1476, 2540
ApeKI	GCWGC	9 cut(s)	850, 1045, 1545, 1988, 2031, 2355, 2358, 2380, 2983
ApoI	RAATTY	5 cut(s)	530, 626, 2113, 2446, 2502
ArsI	GACNNNNNNTTYG	6 cut(s)	907, 939, 1840, 1872, 2765, 2797
AsiGI	ACCGGT	2 cut(s)	1520, 1805
Asp700I	GAANNNNTTC	1 cut(s)	917
AspLEI	GCGC	5 cut(s)	806, 1382, 1886, 1967, 2497
AspS9I	GGNCC	9 cut(s)	228, 371, 515, 1163, 1808, 2025, 2081, 2175, 2950
AsuC2I	CCSGG	3 cut(s)	596, 700, 1028
AsuII	TTCGAA	1 cut(s)	1282
AsuNHI	GCTAGC	1 cut(s)	3013
AvaI	CYCGRG	4 cut(s)	525, 551, 616, 2987
AvaII	GGWCC	7 cut(s)	228, 1163, 1808, 2025, 2081, 2175, 2950
BaeGI	GKGCMC	3 cut(s)	1480, 1617, 2544
BaeI	ACNNNNGTAYC	4 cut(s)	530, 563, 595, 628
BalI	TGGCCA	2 cut(s)	2615, 3033
BamHI	GGATCC	1 cut(s)	411
BanII	GRGCYC	3 cut(s)	399, 822, 1416
BauI	CACGAG	3 cut(s)	1940, 2467, 2932
BbsI	GAAGAC	2 cut(s)	920, 1961
Bbv12I	GWGCWC	5 cut(s)	399, 822, 1416, 1480, 2544
BbvI	GCAGC	9 cut(s)	837, 1032, 1532, 1975, 2018, 2342, 2345, 2367, 2970
BccI	CCATC	5 cut(s)	697, 1851, 2053, 2164, 2737
BcgI	CGANNNNNNTGC	2 cut(s)	346, 380
BciT130I	CCWGG	4 cut(s)	456, 1289, 2144, 2208
BcnI	CCSGG	3 cut(s)	596, 700, 1028
BcoDI	GTCTC	3 cut(s)	214, 787, 2781
BfaI	CTAG	4 cut(s)	893, 2592, 3014, 3018
BfmI	CTRYAG	1 cut(s)	1762
BfoI	RGCGCY	2 cut(s)	807, 1887
BfrI	CTTAAG	3 cut(s)	406, 2552, 2752
BfuAI	ACCTGC	1 cut(s)	2520
BglII	AGATCT	3 cut(s)	1393, 1491, 2748
Bme1390I	CCNGG	7 cut(s)	456, 596, 700, 1028, 1289, 2144, 2208
Bme18I	GGWCC	7 cut(s)	228, 1163, 1808, 2025, 2081, 2175, 2950
BmeRI	GACNNNNNGTC	3 cut(s)	439, 1909, 2956
BmeT110I	CYCGRG	4 cut(s)	525, 551, 616, 2987
BmgBI	CACGTC	2 cut(s)	2131, 2971
BmgT120I	GGNCC	9 cut(s)	228, 371, 515, 1163, 1808, 2025, 2081, 2175, 2950
BmiI	GGNNCC	8 cut(s)	413, 1314, 1921, 1947, 2026, 2027, 2176, 2659
BmrFI	CCNGG	7 cut(s)	456, 596, 700, 1028, 1289, 2144, 2208
BmrI	ACTGGG	1 cut(s)	184
BmsI	GCATC	5 cut(s)	2193, 2342, 2892, 2898, 2970
BmtI	GCTAGC	1 cut(s)	3017
BmuI	ACTGGG	1 cut(s)	184
BoxI	GACNNNNGTC	2 cut(s)	1349, 2067
BpiI	GAAGAC	2 cut(s)	920, 1961
BpmI	CTGGAG	4 cut(s)	44, 1472, 1793, 2165
Bpu10I	CCTNAGC	2 cut(s)	1108, 2912
Bpu14I	TTCGAA	1 cut(s)	1282
BpuEI	CTTGAG	2 cut(s)	119, 455
BpuMI	CCSGG	3 cut(s)	596, 700, 1028
BsaAI	YACGTR	1 cut(s)	771
BsaBI	GATNNNNATC	2 cut(s)	707, 2747
BsaHI	GRCGYC	4 cut(s)	207, 780, 1850, 2775
BsaWI	WCCGGW	9 cut(s)	212, 543, 958, 1160, 1520, 1733, 1805, 1820, 2530
Bsc4I	CCNNNNNNNGG	8 cut(s)	175, 677, 821, 871, 904, 2059, 2491, 2952
Bse118I	RCCGGY	8 cut(s)	512, 1376, 1520, 1661, 1805, 2654, 2884, 2952
Bse1I	ACTGG	2 cut(s)	179, 1503
Bse3DI	GCAATG	7 cut(s)	117, 518, 759, 845, 1192, 1408, 2670
Bse8I	GATNNNNATC	2 cut(s)	707, 2747
BseBI	CCWGG	4 cut(s)	456, 1289, 2144, 2208
BseGI	GGATG	6 cut(s)	42, 708, 1843, 2131, 2175, 2357
BseJI	GATNNNNATC	2 cut(s)	707, 2747
BseLI	CCNNNNNNNGG	8 cut(s)	175, 677, 821, 871, 904, 2059, 2491, 2952
BseMI	GCAATG	7 cut(s)	117, 518, 759, 845, 1192, 1408, 2670
BseMII	CTCAG	7 cut(s)	1429, 1464, 1634, 1645, 2190, 2456, 3045
BseNI	ACTGG	2 cut(s)	179, 1503
BseRI	GAGGAG	7 cut(s)	13, 151, 1055, 1058, 1958, 2301, 2909
BseSI	GKGCMC	3 cut(s)	1480, 1617, 2544
BseX3I	CGGCCG	1 cut(s)	2954
BseXI	GCAGC	9 cut(s)	837, 1032, 1532, 1975, 2018, 2342, 2345, 2367, 2970
BseYI	CCCAGC	1 cut(s)	2021
BsgI	GTGCAG	3 cut(s)	726, 2498, 2914
Bsh1236I	CGCG	5 cut(s)	1382, 1935, 1967, 2042, 2948
Bsh1285I	CGRYCG	2 cut(s)	2772, 2957
BshTI	ACCGGT	2 cut(s)	1520, 1805
BsiEI	CGRYCG	2 cut(s)	2772, 2957
BsiHKAI	GWGCWC	5 cut(s)	399, 822, 1416, 1480, 2544
BsiHKCI	CYCGRG	4 cut(s)	525, 551, 616, 2987
BslFI	GGGAC	5 cut(s)	1619, 2038, 2197, 2381, 2981
BslI	CCNNNNNNNGG	8 cut(s)	175, 677, 821, 871, 904, 2059, 2491, 2952
BsmAI	GTCTC	3 cut(s)	214, 787, 2781
BsmBI	CGTCTC	3 cut(s)	214, 787, 2781
BsmFI	GGGAC	5 cut(s)	1619, 2038, 2197, 2381, 2981
BsmI	GAATGC	1 cut(s)	2869
BsoBI	CYCGRG	4 cut(s)	525, 551, 616, 2987
Bsp119I	TTCGAA	1 cut(s)	1282
Bsp1286I	GDGCHC	6 cut(s)	399, 822, 1416, 1480, 1617, 2544
Bsp1407I	TGTACA	1 cut(s)	1557
Bsp19I	CCATGG	1 cut(s)	1673
BspCNI	CTCAG	7 cut(s)	1428, 1463, 1635, 1644, 2191, 2455, 3046
BspFNI	CGCG	5 cut(s)	1382, 1935, 1967, 2042, 2948
BspHI	TCATGA	1 cut(s)	3061
BspLI	GGNNCC	8 cut(s)	413, 1314, 1921, 1947, 2026, 2027, 2176, 2659
BspMI	ACCTGC	1 cut(s)	2520
BspOI	GCTAGC	1 cut(s)	3017
BspPI	GGATC	8 cut(s)	406, 419, 1162, 1591, 1807, 2266, 2522, 2617
BspQI	GCTCTTC	3 cut(s)	88, 860, 1404
BspT104I	TTCGAA	1 cut(s)	1282
BspTI	CTTAAG	3 cut(s)	406, 2552, 2752
BsrBI	CCGCTC	2 cut(s)	354, 556
BsrDI	GCAATG	7 cut(s)	117, 518, 759, 845, 1192, 1408, 2670
BsrFI	RCCGGY	8 cut(s)	512, 1376, 1520, 1661, 1805, 2654, 2884, 2952
BsrGI	TGTACA	1 cut(s)	1557
BsrI	ACTGG	2 cut(s)	179, 1503
BssAI	RCCGGY	8 cut(s)	512, 1376, 1520, 1661, 1805, 2654, 2884, 2952
BssNI	GRCGYC	4 cut(s)	207, 780, 1850, 2775
BssSI	CACGAG	3 cut(s)	1940, 2467, 2932
BssT1I	CCWWGG	2 cut(s)	565, 1673
Bst2BI	CACGAG	3 cut(s)	1940, 2467, 2932
Bst2UI	CCWGG	4 cut(s)	456, 1289, 2144, 2208
Bst4CI	ACNGT	8 cut(s)	659, 1763, 2085, 2188, 2238, 2261, 2525, 2546
Bst6I	CTCTTC	6 cut(s)	53, 88, 97, 860, 1404, 2278
BstACI	GRCGYC	4 cut(s)	207, 780, 1850, 2775
BstAFI	CTTAAG	3 cut(s)	406, 2552, 2752
BstAUI	TGTACA	1 cut(s)	1557
BstBAI	YACGTR	1 cut(s)	771
BstBI	TTCGAA	1 cut(s)	1282
BstC8I	GCNNGC	7 cut(s)	1476, 1769, 1963, 2656, 2882, 3015, 3087
BstDEI	CTNAG	9 cut(s)	1108, 1415, 1450, 1631, 1643, 2199, 2442, 2912, 3054
BstDSI	CCRYGG	6 cut(s)	1601, 1673, 1923, 2084, 2178, 2946
BstF5I	GGATG	6 cut(s)	42, 708, 1843, 2131, 2175, 2357
BstFNI	CGCG	5 cut(s)	1382, 1935, 1967, 2042, 2948
BstH2I	RGCGCY	2 cut(s)	807, 1887
BstHHI	GCGC	5 cut(s)	806, 1382, 1886, 1967, 2497
BstMAI	GTCTC	3 cut(s)	214, 787, 2781
BstMCI	CGRYCG	2 cut(s)	2772, 2957
BstMWI	GCNNNNNNNGC	9 cut(s)	240, 513, 647, 749, 1204, 1551, 2597, 2664, 3082
BstNI	CCWGG	4 cut(s)	456, 1289, 2144, 2208
BstNSI	RCATGY	1 cut(s)	2398
BstPAI	GACNNNNGTC	2 cut(s)	1349, 2067
BstSCI	CCNGG	7 cut(s)	454, 594, 698, 1026, 1287, 2142, 2206
BstSFI	CTRYAG	1 cut(s)	1762
BstSLI	GKGCMC	3 cut(s)	1480, 1617, 2544
BstUI	CGCG	5 cut(s)	1382, 1935, 1967, 2042, 2948
BstV1I	GCAGC	9 cut(s)	837, 1032, 1532, 1975, 2018, 2342, 2345, 2367, 2970
BstV2I	GAAGAC	2 cut(s)	920, 1961
BstX2I	RGATCY	4 cut(s)	411, 1393, 1491, 2748
BstXI	CCANNNNNNTGG	1 cut(s)	1304
BstYI	RGATCY	4 cut(s)	411, 1393, 1491, 2748
BstZI	CGGCCG	1 cut(s)	2954
BtgI	CCRYGG	6 cut(s)	1601, 1673, 1923, 2084, 2178, 2946
BtgZI	GCGATG	2 cut(s)	2635, 2646
BtrI	CACGTC	2 cut(s)	2131, 2971
BtsCI	GGATG	6 cut(s)	42, 708, 1843, 2131, 2175, 2357
BtsI	GCAGTG	1 cut(s)	1615
BtsIMutI	CAGTG	3 cut(s)	1082, 1123, 1615
BveI	ACCTGC	1 cut(s)	2520
Cac8I	GCNNGC	7 cut(s)	1476, 1769, 1963, 2656, 2882, 3015, 3087
CaiI	CAGNNNCTG	1 cut(s)	910
CciI	TCATGA	1 cut(s)	3061
CfoI	GCGC	5 cut(s)	806, 1382, 1886, 1967, 2497
Cfr10I	RCCGGY	8 cut(s)	512, 1376, 1520, 1661, 1805, 2654, 2884, 2952
Cfr13I	GGNCC	9 cut(s)	228, 371, 515, 1163, 1808, 2025, 2081, 2175, 2950
Cfr42I	CCGCGG	1 cut(s)	2949
CpoI	CGGWCCG	4 cut(s)	1163, 1808, 2081, 2950
CseI	GACGC	2 cut(s)	196, 2783
Csp6I	GTAC	6 cut(s)	604, 715, 772, 1558, 1737, 2392
CspAI	ACCGGT	2 cut(s)	1520, 1805
CspCI	CAANNNNNGTGG	4 cut(s)	657, 692, 3025, 3060
CspI	CGGWCCG	4 cut(s)	1163, 1808, 2081, 2950
CviAII	CATG	8 cut(s)	835, 1674, 2377, 2395, 2426, 2641, 3029, 3062
CviQI	GTAC	6 cut(s)	604, 715, 772, 1558, 1737, 2392
DdeI	CTNAG	9 cut(s)	1108, 1415, 1450, 1631, 1643, 2199, 2442, 2912, 3054
DriI	GACNNNNNGTC	3 cut(s)	439, 1909, 2956
EaeI	YGGCCR	6 cut(s)	298, 1510, 1936, 2613, 2954, 3031
EagI	CGGCCG	1 cut(s)	2954
Eam1104I	CTCTTC	6 cut(s)	53, 88, 97, 860, 1404, 2278
Eam1105I	GACNNNNNGTC	3 cut(s)	439, 1909, 2956
EarI	CTCTTC	6 cut(s)	53, 88, 97, 860, 1404, 2278
EciI	GGCGGA	5 cut(s)	308, 585, 1489, 2138, 2471
Ecl136II	GAGCTC	3 cut(s)	397, 820, 1414
EclXI	CGGCCG	1 cut(s)	2954
Eco130I	CCWWGG	2 cut(s)	565, 1673
Eco24I	GRGCYC	3 cut(s)	399, 822, 1416
Eco47I	GGWCC	7 cut(s)	228, 1163, 1808, 2025, 2081, 2175, 2950
Eco52I	CGGCCG	1 cut(s)	2954
Eco53kI	GAGCTC	3 cut(s)	397, 820, 1414
Eco57I	CTGAAG	4 cut(s)	932, 1106, 1410, 2319
Eco88I	CYCGRG	4 cut(s)	525, 551, 616, 2987
EcoICRI	GAGCTC	3 cut(s)	397, 820, 1414
EcoO109I	RGGNCCY	1 cut(s)	2025
EcoRI	GAATTC	1 cut(s)	530
EcoRII	CCWGG	4 cut(s)	454, 1287, 2142, 2206
EcoT14I	CCWWGG	2 cut(s)	565, 1673
EcoT38I	GRGCYC	3 cut(s)	399, 822, 1416
ErhI	CCWWGG	2 cut(s)	565, 1673
Esp3I	CGTCTC	3 cut(s)	214, 787, 2781
FaeI	CATG	8 cut(s)	838, 1677, 2380, 2398, 2429, 2644, 3032, 3065
FaqI	GGGAC	5 cut(s)	1619, 2038, 2197, 2381, 2981
FatI	CATG	8 cut(s)	834, 1673, 2376, 2394, 2425, 2640, 3028, 3061
FauI	CCCGC	9 cut(s)	391, 459, 819, 1179, 1954, 2036, 2750, 2866, 2953
FblI	GTMKAC	2 cut(s)	1137, 2772
FokI	GGATG	6 cut(s)	29, 715, 1830, 2138, 2182, 2364
FriOI	GRGCYC	3 cut(s)	399, 822, 1416
FspBI	CTAG	4 cut(s)	893, 2592, 3014, 3018
GlaI	GCGC	5 cut(s)	805, 1381, 1885, 1966, 2496
GsaI	CCCAGC	1 cut(s)	2025
GsuI	CTGGAG	4 cut(s)	44, 1472, 1793, 2165
HaeII	RGCGCY	2 cut(s)	807, 1887
HgaI	GACGC	2 cut(s)	196, 2783
HhaI	GCGC	5 cut(s)	806, 1382, 1886, 1967, 2497
Hin1I	GRCGYC	4 cut(s)	207, 780, 1850, 2775
Hin1II	CATG	8 cut(s)	838, 1677, 2380, 2398, 2429, 2644, 3032, 3065
Hin6I	GCGC	5 cut(s)	804, 1380, 1884, 1965, 2495
HinP1I	GCGC	5 cut(s)	804, 1380, 1884, 1965, 2495
HincII	GTYRAC	3 cut(s)	1138, 1803, 2773
HindII	GTYRAC	3 cut(s)	1138, 1803, 2773
HindIII	AAGCTT	1 cut(s)	1625
Hpy166II	GTNNAC	8 cut(s)	1138, 1230, 1478, 1803, 1927, 2178, 2542, 2773
Hpy8I	GTNNAC	8 cut(s)	1138, 1230, 1478, 1803, 1927, 2178, 2542, 2773
HpyAV	CCTTC	9 cut(s)	21, 37, 175, 373, 593, 1081, 1466, 1862, 2245
HpyCH4III	ACNGT	8 cut(s)	659, 1763, 2085, 2188, 2238, 2261, 2525, 2546
HpyF10VI	GCNNNNNNNGC	9 cut(s)	240, 513, 647, 749, 1204, 1551, 2597, 2664, 3082
HpyF3I	CTNAG	9 cut(s)	1108, 1415, 1450, 1631, 1643, 2199, 2442, 2912, 3054
Hsp92I	GRCGYC	4 cut(s)	207, 780, 1850, 2775
Hsp92II	CATG	8 cut(s)	838, 1677, 2380, 2398, 2429, 2644, 3032, 3065
HspAI	GCGC	5 cut(s)	804, 1380, 1884, 1965, 2495
KflI	GGGWCCC	1 cut(s)	2025
KroI	GCCGGC	1 cut(s)	2654
KroNI	GCCGGC	1 cut(s)	2656
KspI	CCGCGG	1 cut(s)	2949
LguI	GCTCTTC	3 cut(s)	88, 860, 1404
LmnI	GCTCC	7 cut(s)	691, 817, 1131, 1203, 1919, 1945, 2663
Lsp1109I	GCAGC	9 cut(s)	837, 1032, 1532, 1975, 2018, 2342, 2345, 2367, 2970
LweI	GCATC	5 cut(s)	2193, 2342, 2892, 2898, 2970
MaeI	CTAG	4 cut(s)	893, 2592, 3014, 3018
MaeIII	GTNAC	6 cut(s)	967, 1332, 1418, 1577, 1901, 2464
MbiI	CCGCTC	2 cut(s)	354, 556
MfeI	CAATTG	1 cut(s)	674
MflI	RGATCY	4 cut(s)	411, 1393, 1491, 2748
MhlI	GDGCHC	6 cut(s)	399, 822, 1416, 1480, 1617, 2544
MlsI	TGGCCA	2 cut(s)	2615, 3033
MluNI	TGGCCA	2 cut(s)	2615, 3033
MlyI	GAGTC	4 cut(s)	534, 1143, 2077, 2645
Mox20I	TGGCCA	2 cut(s)	2615, 3033
MroNI	GCCGGC	1 cut(s)	2654
MroXI	GAANNNNTTC	1 cut(s)	917
MscI	TGGCCA	2 cut(s)	2615, 3033
MslI	CAYNNNNRTG	3 cut(s)	2225, 2375, 2523
Msp20I	TGGCCA	2 cut(s)	2615, 3033
MspA1I	CMGCKG	4 cut(s)	2031, 2107, 2948, 3089
MspCI	CTTAAG	3 cut(s)	406, 2552, 2752
MspR9I	CCNGG	7 cut(s)	456, 596, 700, 1028, 1289, 2144, 2208
MunI	CAATTG	1 cut(s)	674
Mva1269I	GAATGC	1 cut(s)	2869
MvaI	CCWGG	4 cut(s)	456, 1289, 2144, 2208
MvnI	CGCG	5 cut(s)	1382, 1935, 1967, 2042, 2948
MwoI	GCNNNNNNNGC	9 cut(s)	240, 513, 647, 749, 1204, 1551, 2597, 2664, 3082
NaeI	GCCGGC	1 cut(s)	2656
NciI	CCSGG	3 cut(s)	596, 700, 1028
NcoI	CCATGG	1 cut(s)	1673
NgoMIV	GCCGGC	1 cut(s)	2654
NheI	GCTAGC	1 cut(s)	3013
NlaIII	CATG	8 cut(s)	838, 1677, 2380, 2398, 2429, 2644, 3032, 3065
NlaIV	GGNNCC	8 cut(s)	413, 1314, 1921, 1947, 2026, 2027, 2176, 2659
NmeAIII	GCCGAG	5 cut(s)	378, 801, 1377, 2466, 2803
NmuCI	GTSAC	2 cut(s)	1901, 2464
NspI	RCATGY	1 cut(s)	2398
NspV	TTCGAA	1 cut(s)	1282
OliI	CACNNNNGTG	1 cut(s)	2523
PaeR7I	CTCGAG	2 cut(s)	551, 2987
PagI	TCATGA	1 cut(s)	3061
PciSI	GCTCTTC	3 cut(s)	88, 860, 1404
PcsI	WCGNNNNNNNCGW	6 cut(s)	270, 717, 1404, 1617, 1964, 2487
PctI	GAATGC	1 cut(s)	2869
PdiI	GCCGGC	1 cut(s)	2656
PdmI	GAANNNNTTC	1 cut(s)	917
PfeI	GAWTC	6 cut(s)	483, 1113, 1284, 1639, 1741, 1837
PfoI	TCCNGGA	3 cut(s)	594, 1026, 1287
PinAI	ACCGGT	2 cut(s)	1520, 1805
PleI	GAGTC	4 cut(s)	534, 1142, 2076, 2644
PpsI	GAGTC	4 cut(s)	534, 1142, 2076, 2644
Ppu21I	YACGTR	1 cut(s)	771
PpuMI	RGGWCCY	1 cut(s)	2025
PshAI	GACNNNNGTC	2 cut(s)	1349, 2067
Psp124BI	GAGCTC	3 cut(s)	399, 822, 1416
Psp1406I	AACGTT	4 cut(s)	971, 1250, 1785, 2319
Psp5II	RGGWCCY	1 cut(s)	2025
Psp6I	CCWGG	4 cut(s)	454, 1287, 2142, 2206
PspFI	CCCAGC	1 cut(s)	2021
PspGI	CCWGG	4 cut(s)	454, 1287, 2142, 2206
PspN4I	GGNNCC	8 cut(s)	413, 1314, 1921, 1947, 2026, 2027, 2176, 2659
PspPI	GGNCC	9 cut(s)	228, 371, 515, 1163, 1808, 2025, 2081, 2175, 2950
PspPPI	RGGWCCY	1 cut(s)	2025
PspXI	VCTCGAGB	1 cut(s)	2987
PstNI	CAGNNNCTG	1 cut(s)	910
PsuI	RGATCY	4 cut(s)	411, 1393, 1491, 2748
RsaI	GTAC	6 cut(s)	605, 716, 773, 1559, 1738, 2393
RsaNI	GTAC	6 cut(s)	604, 715, 772, 1558, 1737, 2392
RseI	CAYNNNNRTG	3 cut(s)	2225, 2375, 2523
Rsr2I	CGGWCCG	4 cut(s)	1163, 1808, 2081, 2950
RsrII	CGGWCCG	4 cut(s)	1163, 1808, 2081, 2950
SacI	GAGCTC	3 cut(s)	399, 822, 1416
SacII	CCGCGG	1 cut(s)	2949
SalI	GTCGAC	2 cut(s)	1136, 2771
SapI	GCTCTTC	3 cut(s)	88, 860, 1404
Sau96I	GGNCC	9 cut(s)	228, 371, 515, 1163, 1808, 2025, 2081, 2175, 2950
SchI	GAGTC	4 cut(s)	534, 1143, 2077, 2645
ScrFI	CCNGG	7 cut(s)	456, 596, 700, 1028, 1289, 2144, 2208
SduI	GDGCHC	6 cut(s)	399, 822, 1416, 1480, 1617, 2544
SfaNI	GCATC	5 cut(s)	2193, 2342, 2892, 2898, 2970
SfcI	CTRYAG	1 cut(s)	1762
Sfr274I	CTCGAG	2 cut(s)	551, 2987
Sfr303I	CCGCGG	1 cut(s)	2949
SfuI	TTCGAA	1 cut(s)	1282
SgrAI	CRCCGGYG	1 cut(s)	1376
SgrBI	CCGCGG	1 cut(s)	2949
SinI	GGWCC	7 cut(s)	228, 1163, 1808, 2025, 2081, 2175, 2950
SlaI	CTCGAG	2 cut(s)	551, 2987
SmiMI	CAYNNNNRTG	3 cut(s)	2225, 2375, 2523
SmlI	CTYRAG	7 cut(s)	134, 406, 470, 551, 2552, 2752, 2987
SmoI	CTYRAG	7 cut(s)	134, 406, 470, 551, 2552, 2752, 2987
SspMI	CTAG	4 cut(s)	893, 2592, 3014, 3018
SstI	GAGCTC	3 cut(s)	399, 822, 1416
StyD4I	CCNGG	7 cut(s)	454, 594, 698, 1026, 1287, 2142, 2206
StyI	CCWWGG	2 cut(s)	565, 1673
TaaI	ACNGT	8 cut(s)	659, 1763, 2085, 2188, 2238, 2261, 2525, 2546
TaqII	GACCGA	2 cut(s)	1180, 2758
TatI	WGTACW	2 cut(s)	1557, 2391
TfiI	GAWTC	6 cut(s)	483, 1113, 1284, 1639, 1741, 1837
TscAI	CASTG	3 cut(s)	1089, 1123, 1622
TseFI	GTSAC	2 cut(s)	1901, 2464
TseI	GCWGC	9 cut(s)	850, 1045, 1545, 1988, 2031, 2355, 2358, 2380, 2983
Tsp45I	GTSAC	2 cut(s)	1901, 2464
TspDTI	ATGAA	7 cut(s)	17, 523, 1016, 1528, 1881, 2721, 2846
TspGWI	ACGGA	3 cut(s)	621, 1449, 1734
TspRI	CASTG	3 cut(s)	1089, 1123, 1622
Vha464I	CTTAAG	3 cut(s)	406, 2552, 2752
VneI	GTGCAC	2 cut(s)	1476, 2540
VpaK11BI	GGWCC	7 cut(s)	228, 1163, 1808, 2025, 2081, 2175, 2950
XapI	RAATTY	5 cut(s)	530, 626, 2113, 2446, 2502
XceI	RCATGY	1 cut(s)	2398
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3041
XhoI	CTCGAG	2 cut(s)	551, 2987
XmiI	GTMKAC	2 cut(s)	1137, 2772
XmnI	GAANNNNTTC	1 cut(s)	917
XspI	CTAG	4 cut(s)	893, 2592, 3014, 3018
ZraI	GACGTC	2 cut(s)	781, 1851

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.