pycom07g24280

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002987
Physical Location & Seq
Forward (+)
24997711 .. 24998901
1191 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g24280.1

Sequence Viewer

Length: 1086 bp
ATGGAGTGTTTCAGAGAATGGAGAACTCTTCTAAATTTAGCAGTGCCCAGCTGCATTTCTGTGTGTTTAGAATGGTGGTGGTATGAGATCATGATCTTGCTCTGTGGATTGTTAATAAATCCGAGAGCCACCGTTGCTTCAATGGGAGTTTTAATTCAAACGACCGCCTTAATCTACATATTTCCTTCTTCCTTAAGCTTCAGTGTGTCTACCAGAGTCGGCAATGAAATCGGCGCCAACAACCCGAAAAAAGCGAAACTAGCAGCCATTGTAGGGCTCTGCTGCAGCTTTGTGCTAGGCCTTTCAGCTCTAGTGTTTGCAACGATGGTGAGGAACATTTGGGCAAGCATGTTCACACAAGATAAAGAGATTGTAGCGTTGACATCGATGGTATTGCCGATAATCGGGCTCTGCGAGCTCGGAAACTGCCCGCAGACGACGGGGTGCGGAGTTCTGAGAGGCACTGCGAGGCCGAAAGTGGGAGCTAATATAAACTTGGGGTGCTTTTATCTTGTCGGAATGCCGGTGGCCGTGGGGTTGGGATTCTTTCTTGGGTTTGATTTTCAGGGGCTGTGGCTCGGCCTCTTGGCGGCGGAAGGCAGCTGCGCTTTGACCATGTTGGTGGTTTTGGGTCGTACTGATTGGGAGCTTGAAGCTCGGAAAGCCAAAGAGCTGACCGGTGCTGCTGCTGCTAAGGTGGCAGTTGGTGACAGCGAAGGTGTTGAGGAGGACAAGCCAAGCAAAGCTGAAATCAAGGAAGATTGTCTATGTTTATTAGGCGATTTAATCTCTCATTTTTTTTCTTTTCTTTTTTTTTTCTTTTTTTTACATTTTTATTATTTTTATATATATATATATATATATATATATATATATATTTTGAGAATATTTCTATTAGCAGGAGGACTATCTCCAGGAGTGTTTTTTTTTGCACTCCCACTGAAATTCAACTGTACTATTTTACATGTACAACTGATGCTGAATTTCATAGGAAGTAGCAAAATGCAGGGTCAGGAACAAACATCCCACCTTTTTCTGTATTTTCCGACCATTTCTGTATTTTGTAATCGGATACAAAGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0006810 GO:0006855 GO:0008150 GO:0015238 GO:0015893 GO:0022857 GO:0042221 GO:0042493 GO:0050896 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

362

Amino Acids

40.02

Weight (kDa)

6.86

Isoelectric Point (pI)

35.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 16 - 177 3e-37 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 233
AccI GTMKAC 1 cut(s) 209
AciI CCGC 5 cut(s) 165, 431, 447, 590, 593
AcoI YGGCCR 1 cut(s) 528
AcsI RAATTY 3 cut(s) 34, 944, 982
AcuI CTGAAG 1 cut(s) 184
AcyI GRCGYC 1 cut(s) 234
AfaI GTAC 3 cut(s) 637, 955, 969
AfiI CCNNNNNNNGG 4 cut(s) 273, 404, 479, 589
AflII CTTAAG 1 cut(s) 193
AflIII ACRYGT 1 cut(s) 964
AgeI ACCGGT 1 cut(s) 677
AgsI TTSAA 4 cut(s) 141, 158, 653, 949
AjnI CCWGG 1 cut(s) 913
Alw21I GWGCWC 1 cut(s) 420
AlwNI CAGNNNCTG 1 cut(s) 571
AoxI GGCC 4 cut(s) 298, 470, 528, 580
ApeKI GCWGC 9 cut(s) 51, 263, 282, 285, 600, 603, 683, 686, 689
ApoI RAATTY 3 cut(s) 34, 944, 982
AsiGI ACCGGT 1 cut(s) 677
AspLEI GCGC 2 cut(s) 236, 608
AsuHPI GGTGA 2 cut(s) 340, 719
BaeGI GKGCMC 1 cut(s) 48
BanI GGYRCC 1 cut(s) 233
BanII GRGCYC 3 cut(s) 279, 411, 420
Bbv12I GWGCWC 1 cut(s) 420
BbvI GCAGC 9 cut(s) 38, 269, 275, 297, 590, 612, 670, 673, 676
BccI CCATC 2 cut(s) 319, 382
BceAI ACGGC 1 cut(s) 515
BcgI CGANNNNNNTGC 4 cut(s) 211, 245, 376, 410
BciT130I CCWGG 1 cut(s) 915
BciVI GTATCC 1 cut(s) 1065
BfaI CTAG 3 cut(s) 260, 296, 311
BfmI CTRYAG 1 cut(s) 283
BfoI RGCGCY 1 cut(s) 237
BfrI CTTAAG 1 cut(s) 193
BfuI GTATCC 1 cut(s) 1065
Bme1390I CCNGG 1 cut(s) 915
BmiI GGNNCC 1 cut(s) 235
BmrFI CCNGG 1 cut(s) 915
BmsI GCATC 1 cut(s) 966
BpmI CTGGAG 1 cut(s) 897
Bpu10I CCTNAGC 1 cut(s) 693
Bsa29I ATCGAT 1 cut(s) 386
BsaBI GATNNNNATC 1 cut(s) 92
BsaHI GRCGYC 1 cut(s) 234
BsaJI CCNNGG 1 cut(s) 531
BsaWI WCCGGW 1 cut(s) 677
Bsc4I CCNNNNNNNGG 4 cut(s) 273, 404, 479, 589
Bse118I RCCGGY 2 cut(s) 523, 677
Bse3DI GCAATG 1 cut(s) 229
Bse8I GATNNNNATC 1 cut(s) 92
BseBI CCWGG 1 cut(s) 915
BseCI ATCGAT 1 cut(s) 386
BseDI CCNNGG 1 cut(s) 531
BseGI GGATG 1 cut(s) 1022
BseJI GATNNNNATC 1 cut(s) 92
BseLI CCNNNNNNNGG 4 cut(s) 273, 404, 479, 589
BseMI GCAATG 1 cut(s) 229
BseMII CTCAG 1 cut(s) 446
BseRI GAGGAG 1 cut(s) 740
BseSI GKGCMC 1 cut(s) 48
BseXI GCAGC 9 cut(s) 38, 269, 275, 297, 590, 612, 670, 673, 676
BseYI CCCAGC 1 cut(s) 47
Bsh1285I CGRYCG 1 cut(s) 165
BshFI GGCC 4 cut(s) 300, 472, 530, 582
BshNI GGYRCC 1 cut(s) 233
BshTI ACCGGT 1 cut(s) 677
BshVI ATCGAT 1 cut(s) 386
BsiEI CGRYCG 1 cut(s) 165
BsiHKAI GWGCWC 1 cut(s) 420
BsiSI CCGG 2 cut(s) 524, 678
BslI CCNNNNNNNGG 4 cut(s) 273, 404, 479, 589
BsmI GAATGC 1 cut(s) 525
BsnI GGCC 4 cut(s) 300, 472, 530, 582
Bsp1286I GDGCHC 4 cut(s) 48, 279, 411, 420
Bsp1407I TGTACA 1 cut(s) 967
Bsp143I GATC 2 cut(s) 87, 93
BspACI CCGC 5 cut(s) 165, 431, 447, 590, 593
BspANI GGCC 4 cut(s) 300, 472, 530, 582
BspCNI CTCAG 1 cut(s) 447
BspDI ATCGAT 1 cut(s) 386
BspHI TCATGA 1 cut(s) 90
BspLI GGNNCC 1 cut(s) 235
BspMAI CTGCAG 1 cut(s) 287
BspT107I GGYRCC 1 cut(s) 233
BspTI CTTAAG 1 cut(s) 193
BsrDI GCAATG 1 cut(s) 229
BsrFI RCCGGY 2 cut(s) 523, 677
BsrGI TGTACA 1 cut(s) 967
BssAI RCCGGY 2 cut(s) 523, 677
BssECI CCNNGG 1 cut(s) 531
BssMI GATC 2 cut(s) 87, 93
BssNI GRCGYC 1 cut(s) 234
Bst2UI CCWGG 1 cut(s) 915
Bst4CI ACNGT 2 cut(s) 133, 953
Bst6I CTCTTC 1 cut(s) 33
BstACI GRCGYC 1 cut(s) 234
BstAFI CTTAAG 1 cut(s) 193
BstAUI TGTACA 1 cut(s) 967
BstC8I GCNNGC 3 cut(s) 346, 416, 431
BstDEI CTNAG 2 cut(s) 455, 693
BstDSI CCRYGG 1 cut(s) 531
BstF5I GGATG 1 cut(s) 1022
BstH2I RGCGCY 1 cut(s) 237
BstHHI GCGC 2 cut(s) 236, 608
BstKTI GATC 2 cut(s) 90, 96
BstMBI GATC 2 cut(s) 87, 93
BstMCI CGRYCG 1 cut(s) 165
BstMWI GCNNNNNNNGC 6 cut(s) 134, 260, 415, 662, 689, 698
BstNI CCWGG 1 cut(s) 915
BstNSI RCATGY 2 cut(s) 352, 968
BstSCI CCNGG 1 cut(s) 913
BstSFI CTRYAG 1 cut(s) 283
BstSLI GKGCMC 1 cut(s) 48
BstV1I GCAGC 9 cut(s) 38, 269, 275, 297, 590, 612, 670, 673, 676
BstXI CCANNNNNNTGG 1 cut(s) 622
Bsu15I ATCGAT 1 cut(s) 386
BsuI GTATCC 1 cut(s) 1065
BsuRI GGCC 4 cut(s) 300, 472, 530, 582
BsuTUI ATCGAT 1 cut(s) 386
BtgI CCRYGG 1 cut(s) 531
BtsCI GGATG 1 cut(s) 1022
BtsI GCAGTG 2 cut(s) 48, 462
BtsIMutI CAGTG 4 cut(s) 48, 208, 462, 938
Cac8I GCNNGC 3 cut(s) 346, 416, 431
CaiI CAGNNNCTG 1 cut(s) 571
CciI TCATGA 1 cut(s) 90
CfoI GCGC 2 cut(s) 236, 608
Cfr10I RCCGGY 2 cut(s) 523, 677
ClaI ATCGAT 1 cut(s) 386
Csp6I GTAC 3 cut(s) 636, 954, 968
CspAI ACCGGT 1 cut(s) 677
CviAII CATG 4 cut(s) 91, 349, 616, 965
CviQI GTAC 3 cut(s) 636, 954, 968
DdeI CTNAG 2 cut(s) 455, 693
DinI GGCGCC 1 cut(s) 235
DpnI GATC 2 cut(s) 89, 95
DpnII GATC 2 cut(s) 87, 93
EaeI YGGCCR 1 cut(s) 528
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
EciI GGCGGA 1 cut(s) 608
Ecl136II GAGCTC 1 cut(s) 418
Eco147I AGGCCT 1 cut(s) 300
Eco24I GRGCYC 3 cut(s) 279, 411, 420
Eco53kI GAGCTC 1 cut(s) 418
Eco57I CTGAAG 1 cut(s) 184
EcoICRI GAGCTC 1 cut(s) 418
EcoRII CCWGG 1 cut(s) 913
EcoT38I GRGCYC 3 cut(s) 279, 411, 420
EgeI GGCGCC 1 cut(s) 235
EheI GGCGCC 1 cut(s) 235
FaeI CATG 4 cut(s) 94, 352, 619, 968
FatI CATG 4 cut(s) 90, 348, 615, 964
FauI CCCGC 1 cut(s) 438
FblI GTMKAC 1 cut(s) 209
FokI GGATG 1 cut(s) 1009
FriOI GRGCYC 3 cut(s) 279, 411, 420
FspBI CTAG 3 cut(s) 260, 296, 311
GlaI GCGC 2 cut(s) 235, 607
GsaI CCCAGC 1 cut(s) 51
GsuI CTGGAG 1 cut(s) 897
HaeII RGCGCY 1 cut(s) 237
HaeIII GGCC 4 cut(s) 300, 472, 530, 582
HapII CCGG 2 cut(s) 524, 678
HhaI GCGC 2 cut(s) 236, 608
Hin1I GRCGYC 1 cut(s) 234
Hin1II CATG 4 cut(s) 94, 352, 619, 968
Hin6I GCGC 2 cut(s) 234, 606
HinP1I GCGC 2 cut(s) 234, 606
HincII GTYRAC 1 cut(s) 381
HindII GTYRAC 1 cut(s) 381
HindIII AAGCTT 1 cut(s) 196
HinfI GANTC 2 cut(s) 216, 543
HpaII CCGG 2 cut(s) 524, 678
HphI GGTGA 2 cut(s) 340, 719
Hpy166II GTNNAC 3 cut(s) 210, 354, 381
Hpy188I TCNGA 8 cut(s) 14, 123, 422, 456, 518, 660, 1047, 1071
Hpy188III TCNNGA 2 cut(s) 91, 1013
Hpy8I GTNNAC 3 cut(s) 210, 354, 381
Hpy99I CGWCG 1 cut(s) 442
HpyAV CCTTC 3 cut(s) 195, 590, 710
HpyCH4III ACNGT 2 cut(s) 133, 953
HpyCH4V TGCA 5 cut(s) 54, 285, 320, 932, 1006
HpyF10VI GCNNNNNNNGC 6 cut(s) 134, 260, 415, 662, 689, 698
HpyF3I CTNAG 2 cut(s) 455, 693
Hsp92I GRCGYC 1 cut(s) 234
Hsp92II CATG 4 cut(s) 94, 352, 619, 968
HspAI GCGC 2 cut(s) 234, 606
KasI GGCGCC 1 cut(s) 233
Kzo9I GATC 2 cut(s) 87, 93
LmnI GCTCC 2 cut(s) 482, 646
Lsp1109I GCAGC 9 cut(s) 38, 269, 275, 297, 590, 612, 670, 673, 676
LweI GCATC 1 cut(s) 966
MaeI CTAG 3 cut(s) 260, 296, 311
MaeIII GTNAC 1 cut(s) 707
MalI GATC 2 cut(s) 89, 95
MboI GATC 2 cut(s) 87, 93
MboII GAAGA 3 cut(s) 20, 180, 770
MhlI GDGCHC 4 cut(s) 48, 279, 411, 420
MluCI AATT 5 cut(s) 34, 153, 944, 982, 1081
Mly113I GGCGCC 1 cut(s) 234
MlyI GAGTC 1 cut(s) 225
MmeI TCCRAC 2 cut(s) 496, 1070
MnlI CCTC 7 cut(s) 324, 452, 462, 593, 718, 721, 896
MseI TTAA 5 cut(s) 113, 152, 170, 194, 785
MslI CAYNNNNRTG 2 cut(s) 59, 620
MspA1I CMGCKG 2 cut(s) 51, 603
MspCI CTTAAG 1 cut(s) 193
MspI CCGG 2 cut(s) 524, 678
MspR9I CCNGG 1 cut(s) 915
Mva1269I GAATGC 1 cut(s) 525
MvaI CCWGG 1 cut(s) 915
MwoI GCNNNNNNNGC 6 cut(s) 134, 260, 415, 662, 689, 698
NarI GGCGCC 1 cut(s) 234
NdeII GATC 2 cut(s) 87, 93
NlaIII CATG 4 cut(s) 94, 352, 619, 968
NlaIV GGNNCC 1 cut(s) 235
NmeAIII GCCGAG 1 cut(s) 558
NmuCI GTSAC 1 cut(s) 707
NspI RCATGY 2 cut(s) 352, 968
PagI TCATGA 1 cut(s) 90
PceI AGGCCT 1 cut(s) 300
PciI ACATGT 1 cut(s) 964
PcsI WCGNNNNNNNCGW 1 cut(s) 411
PctI GAATGC 1 cut(s) 525
PfeI GAWTC 1 cut(s) 543
PfoI TCCNGGA 1 cut(s) 913
PinAI ACCGGT 1 cut(s) 677
PleI GAGTC 1 cut(s) 224
PluTI GGCGCC 1 cut(s) 237
PpsI GAGTC 1 cut(s) 224
PscI ACATGT 1 cut(s) 964
Psp124BI GAGCTC 1 cut(s) 420
Psp6I CCWGG 1 cut(s) 913
PspFI CCCAGC 1 cut(s) 47
PspGI CCWGG 1 cut(s) 913
PspN4I GGNNCC 1 cut(s) 235
PstI CTGCAG 1 cut(s) 287
PstNI CAGNNNCTG 1 cut(s) 571
PvuII CAGCTG 2 cut(s) 51, 603
RsaI GTAC 3 cut(s) 637, 955, 969
RsaNI GTAC 3 cut(s) 636, 954, 968
RseI CAYNNNNRTG 2 cut(s) 59, 620
SacI GAGCTC 1 cut(s) 420
SaqAI TTAA 5 cut(s) 113, 152, 170, 194, 785
Sau3AI GATC 2 cut(s) 87, 93
SchI GAGTC 1 cut(s) 225
ScrFI CCNGG 1 cut(s) 915
SduI GDGCHC 4 cut(s) 48, 279, 411, 420
SfaNI GCATC 1 cut(s) 966
SfcI CTRYAG 1 cut(s) 283
SfoI GGCGCC 1 cut(s) 235
SmiMI CAYNNNNRTG 2 cut(s) 59, 620
SmlI CTYRAG 1 cut(s) 193
SmoI CTYRAG 1 cut(s) 193
Sse9I AATT 5 cut(s) 34, 153, 944, 982, 1081
SseBI AGGCCT 1 cut(s) 300
SsiI CCGC 5 cut(s) 165, 431, 447, 590, 593
SspDI GGCGCC 1 cut(s) 233
SspI AATATT 1 cut(s) 888
SspMI CTAG 3 cut(s) 260, 296, 311
SstI GAGCTC 1 cut(s) 420
StuI AGGCCT 1 cut(s) 300
StyD4I CCNGG 1 cut(s) 913
TaaI ACNGT 2 cut(s) 133, 953
TaqI TCGA 1 cut(s) 386
TasI AATT 5 cut(s) 34, 153, 944, 982, 1081
TatI WGTACW 2 cut(s) 953, 967
TauI GCSGC 1 cut(s) 593
TfiI GAWTC 1 cut(s) 543
Tru1I TTAA 5 cut(s) 113, 152, 170, 194, 785
Tru9I TTAA 5 cut(s) 113, 152, 170, 194, 785
TscAI CASTG 4 cut(s) 48, 208, 469, 945
TseFI GTSAC 1 cut(s) 707
TseI GCWGC 9 cut(s) 51, 263, 282, 285, 600, 603, 683, 686, 689
Tsp45I GTSAC 1 cut(s) 707
TspDTI ATGAA 2 cut(s) 240, 976
TspRI CASTG 4 cut(s) 48, 208, 469, 945
Vha464I CTTAAG 1 cut(s) 193
XapI RAATTY 3 cut(s) 34, 944, 982
XceI RCATGY 2 cut(s) 352, 968
XmiI GTMKAC 1 cut(s) 209
XspI CTAG 3 cut(s) 260, 296, 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.