pycom08g00040

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0015325
Physical Location & Seq
Reverse (-)
59337 .. 62618
3282 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1308 bp
ATGTTTTACAATTGCCATCCGCTTTGGAGATTAAACATCAATAACAAAGGAAACGAACAAGAAAGCGAAAGTAAGAGGGAGAGGGAAGAAATGATGGGGTTCACGGTAAGGGCTCCCCGTCCAGTTTCGTTTGGGCCGCCGCTCTATTTGCAGTTGCGGCGATTCCCAAGCAAGCACATAGGAATAAGGAGCTTGCTTTTTAGATATGGTGGTCAAAATATTCTTCCTCGCAGCTTGAACATGAAATTCAGCTCTTGTGTTTCTGCAGCAAAGGCAACGTCGTCTAGTATTACAGCAGCGGCCACAGGTGCAGCAGCTGGTGGCGAAGATACGACGACTTCGTTGGAAGTGGAGGAGTTGAGAGAGATAAAAGACAAGTGCAGACAGTGGAAGTGGAAGGGTAGATACGCCATCAATTACTTTGTTTCTCTTTCTGATCCACCACCATCATCTTCAAATCCAAAACCGAAGCCTTCTCTACTGCTTGTCCACGGTTTCGGAGCCTCCATTCCTCACTGGCGAAGAAACATTAGGACGTTGTCCCAGAATTATAGCGTTTATGCCATTGACCTCCTTGGCTTTGGCGCCTCGGAAAAGCCTACTGGATTCTCATATACCATGGAAAAATGGGCTAAGTTAATCTTGGATTTTCTGGACGAAATAGTTCAAAAGCCAACTGTGTTGATAGGGAACTCTGTTGGAAGCCTTGCTTGTGTAATTGCTGCCTCAGAATCTAATAATAAAGCACTTGTTGGAGGGTTAGTGCTTTTAAATTGTGCTGGTGGTATGAATAATAAAGCAATTGTTGATGATTGGAGGATCAAGCTTTTGTTACCTTTGCTTTGGTTTTTTGACTTTCTACTCAAACAACGACGAATTGCTTCGGCCATTTTTGAGCGTGTCAAACAAAGAGATACTATAAGGAAGGCTTTATTGGCTGTTTATGGGAACAAGGAATCCGTCGATAAAGAATTGGTAGAGATAATCATGGAACCAACGTGCGACCCAGGGGCACTTGATGCGTTTGTTTCTATCGTGACTGGCCCCCCAGGACCAAACCCGATGCAGTTGATATCAAGAATTTCCGATATCCCTCTTCTAGTATTATGGGGGGATCAAGACCCCTTCACCCCGCTTGATGGACCTCTTGGCAAATACTTCGCTTCCCTGCCTTCTCAACTTCCCCCCAATGCGAATGCGAATGTAAGCTTCTTTGTTCTGCCAGGGGTTGGACATTGCCCCCATGATGACAGGCCCGAGTTAGTTCACGAAAGACTTTTACCCTGGTTGGATCACCTTTCTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

436

Amino Acids

48.37

Weight (kDa)

8.94

Isoelectric Point (pI)

45.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 155 - 323 2.7e-11 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 158 - 419 2.5e-22 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 160 - 423 4.6e-21 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015325)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38520 AT5G38520
fragaria_vesca FvH4_2g37190
malus_domestica MD08G1001000.v1.1
prunus_persica Prupe.1G353100_v2.0.a1
pyrus_communis pycom08g00040
rosa_chinensis RchiOBHm_Chr6g0304501
rosa_laevigata RLG00000010971
rosa_multiflora Rmu_sc0023263.1_g000001 Rmu_ssc0000218.1_g000058
rosa_roxburghii Rroxscaffold_7G00163690
rosa_rugosa Rorug06G0332600
rosa_samantha Rh6AG445100 Rh6CG457900 Rh6DG444700
rosa_wichuraiana Rw6G038640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 584
AccB7I CCANNNNNTGG 1 cut(s) 1229
AccBSI CCGCTC 1 cut(s) 142
AciI CCGC 6 cut(s) 20, 137, 140, 157, 299, 1133
AclWI GGATC 4 cut(s) 431, 827, 1122, 1299
AcoI YGGCCR 2 cut(s) 300, 885
AcsI RAATTY 2 cut(s) 245, 1080
AcyI GRCGYC 1 cut(s) 585
AfiI CCNNNNNNNGG 2 cut(s) 1139, 1229
AgsI TTSAA 3 cut(s) 238, 456, 668
AjnI CCWGG 4 cut(s) 1006, 1048, 1222, 1283
AjuI GAANNNNNNNTTGG 2 cut(s) 917, 949
AloI GAACNNNNNNTCC 2 cut(s) 941, 973
AluBI AGCT 6 cut(s) 192, 234, 252, 317, 826, 1209
AluI AGCT 6 cut(s) 192, 234, 252, 317, 826, 1209
AlwI GGATC 4 cut(s) 431, 827, 1122, 1299
AlwNI CAGNNNCTG 1 cut(s) 317
Ama87I CYCGRG 1 cut(s) 1256
AoxI GGCC 5 cut(s) 134, 300, 885, 1042, 1253
ApeKI GCWGC 6 cut(s) 231, 266, 296, 311, 314, 722
ApoI RAATTY 2 cut(s) 245, 1080
ArsI GACNNNNNNTTYG 2 cut(s) 263, 295
Asp700I GAANNNNTTC 2 cut(s) 663, 880
AspLEI GCGC 1 cut(s) 587
AspS9I GGNCC 5 cut(s) 134, 1043, 1052, 1142, 1254
AsuHPI GGTGA 2 cut(s) 1120, 1286
AvaI CYCGRG 1 cut(s) 1256
AvaII GGWCC 2 cut(s) 1052, 1142
BaeGI GKGCMC 1 cut(s) 1015
BanI GGYRCC 1 cut(s) 584
BanII GRGCYC 1 cut(s) 115
BarI GAAGNNNNNNTAC 2 cut(s) 389, 421
BbvI GCAGC 6 cut(s) 243, 278, 308, 323, 326, 709
BccI CCATC 5 cut(s) 24, 88, 419, 454, 1133
BcgI CGANNNNNNTGC 2 cut(s) 1141, 1175
BciT130I CCWGG 4 cut(s) 1008, 1050, 1224, 1285
BfaI CTAG 2 cut(s) 285, 1100
BfmI CTRYAG 1 cut(s) 264
BfoI RGCGCY 1 cut(s) 588
Bme1390I CCNGG 4 cut(s) 1008, 1050, 1224, 1285
Bme18I GGWCC 2 cut(s) 1052, 1142
BmeT110I CYCGRG 1 cut(s) 1256
BmgT120I GGNCC 5 cut(s) 134, 1043, 1052, 1142, 1254
BmiI GGNNCC 5 cut(s) 114, 502, 586, 993, 1045
BmrFI CCNGG 4 cut(s) 1008, 1050, 1224, 1285
BmsI GCATC 2 cut(s) 1009, 1053
BsaHI GRCGYC 1 cut(s) 585
BsaJI CCNNGG 9 cut(s) 490, 574, 588, 618, 1006, 1007, 1048, 1223, 1283
BsaXI ACNNNNNCTCC 2 cut(s) 747, 777
Bsc4I CCNNNNNNNGG 2 cut(s) 1139, 1229
Bse1I ACTGG 4 cut(s) 122, 521, 607, 1045
Bse3DI GCAATG 1 cut(s) 1234
BseBI CCWGG 4 cut(s) 1008, 1050, 1224, 1285
BseDI CCNNGG 9 cut(s) 490, 574, 588, 618, 1006, 1007, 1048, 1223, 1283
BseGI GGATG 1 cut(s) 16
BseLI CCNNNNNNNGG 2 cut(s) 1139, 1229
BseMI GCAATG 1 cut(s) 1234
BseMII CTCAG 1 cut(s) 741
BseNI ACTGG 4 cut(s) 122, 521, 607, 1045
BseRI GAGGAG 1 cut(s) 368
BseSI GKGCMC 1 cut(s) 1015
BseXI GCAGC 6 cut(s) 243, 278, 308, 323, 326, 709
BsgI GTGCAG 2 cut(s) 330, 400
BshFI GGCC 5 cut(s) 136, 302, 887, 1044, 1255
BshNI GGYRCC 1 cut(s) 584
BsiHKCI CYCGRG 1 cut(s) 1256
BslFI GGGAC 1 cut(s) 526
BslI CCNNNNNNNGG 2 cut(s) 1139, 1229
BsmFI GGGAC 1 cut(s) 526
BsmI GAATGC 1 cut(s) 1201
BsnI GGCC 5 cut(s) 136, 302, 887, 1044, 1255
BsoBI CYCGRG 1 cut(s) 1256
Bsp1286I GDGCHC 2 cut(s) 115, 1015
Bsp143I GATC 4 cut(s) 436, 819, 1114, 1291
Bsp19I CCATGG 1 cut(s) 618
BspACI CCGC 6 cut(s) 20, 137, 140, 157, 299, 1133
BspANI GGCC 5 cut(s) 136, 302, 887, 1044, 1255
BspCNI CTCAG 1 cut(s) 740
BspLI GGNNCC 5 cut(s) 114, 502, 586, 993, 1045
BspMAI CTGCAG 1 cut(s) 268
BspPI GGATC 4 cut(s) 431, 827, 1122, 1299
BspT107I GGYRCC 1 cut(s) 584
BsrBI CCGCTC 1 cut(s) 142
BsrDI GCAATG 1 cut(s) 1234
BsrI ACTGG 4 cut(s) 122, 521, 607, 1045
BssECI CCNNGG 9 cut(s) 490, 574, 588, 618, 1006, 1007, 1048, 1223, 1283
BssMI GATC 4 cut(s) 436, 819, 1114, 1291
BssNI GRCGYC 1 cut(s) 585
BssT1I CCWWGG 2 cut(s) 574, 618
Bst2UI CCWGG 4 cut(s) 1008, 1050, 1224, 1285
Bst4CI ACNGT 4 cut(s) 106, 387, 494, 679
Bst6I CTCTTC 1 cut(s) 1101
BstACI GRCGYC 1 cut(s) 585
BstAPI GCANNNNNTGC 1 cut(s) 1019
BstC8I GCNNGC 2 cut(s) 173, 194
BstDEI CTNAG 2 cut(s) 633, 727
BstDSI CCRYGG 2 cut(s) 490, 618
BstF5I GGATG 1 cut(s) 16
BstH2I RGCGCY 1 cut(s) 588
BstHHI GCGC 1 cut(s) 587
BstKTI GATC 4 cut(s) 439, 822, 1117, 1294
BstMBI GATC 4 cut(s) 436, 819, 1114, 1291
BstMWI GCNNNNNNNGC 6 cut(s) 148, 157, 272, 308, 935, 1019
BstNI CCWGG 4 cut(s) 1008, 1050, 1224, 1285
BstSCI CCNGG 4 cut(s) 1006, 1048, 1222, 1283
BstSFI CTRYAG 1 cut(s) 264
BstSLI GKGCMC 1 cut(s) 1015
BstV1I GCAGC 6 cut(s) 243, 278, 308, 323, 326, 709
BsuRI GGCC 5 cut(s) 136, 302, 887, 1044, 1255
BtgI CCRYGG 2 cut(s) 490, 618
BtsCI GGATG 1 cut(s) 16
BtsIMutI CAGTG 2 cut(s) 392, 514
Cac8I GCNNGC 2 cut(s) 173, 194
CaiI CAGNNNCTG 1 cut(s) 317
CfoI GCGC 1 cut(s) 587
Cfr13I GGNCC 5 cut(s) 134, 1043, 1052, 1142, 1254
CviAII CATG 4 cut(s) 241, 619, 988, 1244
DdeI CTNAG 2 cut(s) 633, 727
DinI GGCGCC 1 cut(s) 586
DpnI GATC 4 cut(s) 438, 821, 1116, 1293
DpnII GATC 4 cut(s) 436, 819, 1114, 1291
DraI TTTAAA 1 cut(s) 771
EaeI YGGCCR 2 cut(s) 300, 885
Eam1104I CTCTTC 1 cut(s) 1101
EarI CTCTTC 1 cut(s) 1101
Eco130I CCWWGG 2 cut(s) 574, 618
Eco24I GRGCYC 1 cut(s) 115
Eco32I GATATC 2 cut(s) 1074, 1090
Eco47I GGWCC 2 cut(s) 1052, 1142
Eco88I CYCGRG 1 cut(s) 1256
EcoRII CCWGG 4 cut(s) 1006, 1048, 1222, 1283
EcoRV GATATC 2 cut(s) 1074, 1090
EcoT14I CCWWGG 2 cut(s) 574, 618
EcoT38I GRGCYC 1 cut(s) 115
EgeI GGCGCC 1 cut(s) 586
EheI GGCGCC 1 cut(s) 586
ErhI CCWWGG 2 cut(s) 574, 618
FaeI CATG 4 cut(s) 244, 622, 991, 1247
FalI AAGNNNNNCTT 6 cut(s) 626, 658, 694, 726, 913, 945
FaqI GGGAC 1 cut(s) 526
FatI CATG 4 cut(s) 240, 618, 987, 1243
FauI CCCGC 1 cut(s) 1140
FokI GGATG 1 cut(s) 3
FriOI GRGCYC 1 cut(s) 115
FspBI CTAG 2 cut(s) 285, 1100
GlaI GCGC 1 cut(s) 586
HaeII RGCGCY 1 cut(s) 588
HaeIII GGCC 5 cut(s) 136, 302, 887, 1044, 1255
HhaI GCGC 1 cut(s) 587
Hin1I GRCGYC 1 cut(s) 585
Hin1II CATG 4 cut(s) 244, 622, 991, 1247
Hin6I GCGC 1 cut(s) 585
HinP1I GCGC 1 cut(s) 585
HindIII AAGCTT 2 cut(s) 824, 1207
HinfI GANTC 4 cut(s) 162, 606, 731, 956
HphI GGTGA 2 cut(s) 1120, 1286
Hpy166II GTNNAC 3 cut(s) 102, 490, 1267
Hpy188I TCNGA 5 cut(s) 436, 500, 592, 730, 1087
Hpy188III TCNNGA 5 cut(s) 653, 1036, 1077, 1118, 1268
Hpy8I GTNNAC 3 cut(s) 102, 490, 1267
Hpy99I CGWCG 4 cut(s) 283, 337, 876, 965
HpyAV CCTTC 5 cut(s) 391, 483, 919, 1135, 1182
HpyCH4III ACNGT 4 cut(s) 106, 387, 494, 679
HpyCH4IV ACGT 3 cut(s) 278, 536, 998
HpyCH4V TGCA 5 cut(s) 151, 266, 311, 381, 1066
HpyF10VI GCNNNNNNNGC 6 cut(s) 148, 157, 272, 308, 935, 1019
HpyF3I CTNAG 2 cut(s) 633, 727
HpySE526I ACGT 3 cut(s) 278, 536, 998
Hsp92I GRCGYC 1 cut(s) 585
Hsp92II CATG 4 cut(s) 244, 622, 991, 1247
HspAI GCGC 1 cut(s) 585
KasI GGCGCC 1 cut(s) 584
Kzo9I GATC 4 cut(s) 436, 819, 1114, 1291
LmnI GCTCC 3 cut(s) 118, 189, 500
Lsp1109I GCAGC 6 cut(s) 243, 278, 308, 323, 326, 709
LweI GCATC 2 cut(s) 1009, 1053
MaeI CTAG 2 cut(s) 285, 1100
MaeII ACGT 3 cut(s) 278, 536, 998
MaeIII GTNAC 2 cut(s) 831, 1036
MalI GATC 4 cut(s) 438, 821, 1116, 1293
MbiI CCGCTC 1 cut(s) 142
MboI GATC 4 cut(s) 436, 819, 1114, 1291
MboII GAAGA 6 cut(s) 98, 215, 338, 444, 534, 1088
MfeI CAATTG 2 cut(s) 10, 801
MhlI GDGCHC 2 cut(s) 115, 1015
Mly113I GGCGCC 1 cut(s) 585
MmeI TCCRAC 5 cut(s) 324, 679, 733, 1210, 1269
MroXI GAANNNNTTC 2 cut(s) 663, 880
MseI TTAA 4 cut(s) 32, 638, 770, 1306
MspA1I CMGCKG 2 cut(s) 299, 317
MspR9I CCNGG 4 cut(s) 1008, 1050, 1224, 1285
MunI CAATTG 2 cut(s) 10, 801
Mva1269I GAATGC 1 cut(s) 1201
MvaI CCWGG 4 cut(s) 1008, 1050, 1224, 1285
MwoI GCNNNNNNNGC 6 cut(s) 148, 157, 272, 308, 935, 1019
NarI GGCGCC 1 cut(s) 585
NcoI CCATGG 1 cut(s) 618
NdeII GATC 4 cut(s) 436, 819, 1114, 1291
NlaIII CATG 4 cut(s) 244, 622, 991, 1247
NlaIV GGNNCC 5 cut(s) 114, 502, 586, 993, 1045
NmuCI GTSAC 1 cut(s) 1036
PasI CCCWGGG 1 cut(s) 1007
PcsI WCGNNNNNNNCGW 1 cut(s) 338
PctI GAATGC 1 cut(s) 1201
PdmI GAANNNNTTC 2 cut(s) 663, 880
PfeI GAWTC 4 cut(s) 162, 606, 731, 956
PflFI GACNNNGTC 1 cut(s) 538
PflMI CCANNNNNTGG 1 cut(s) 1229
PluTI GGCGCC 1 cut(s) 588
Psp6I CCWGG 4 cut(s) 1006, 1048, 1222, 1283
PspGI CCWGG 4 cut(s) 1006, 1048, 1222, 1283
PspN4I GGNNCC 5 cut(s) 114, 502, 586, 993, 1045
PspPI GGNCC 5 cut(s) 134, 1043, 1052, 1142, 1254
PstI CTGCAG 1 cut(s) 268
PstNI CAGNNNCTG 1 cut(s) 317
PsyI GACNNNGTC 1 cut(s) 538
PvuII CAGCTG 1 cut(s) 317
SaqAI TTAA 4 cut(s) 32, 638, 770, 1306
Sau3AI GATC 4 cut(s) 436, 819, 1114, 1291
Sau96I GGNCC 5 cut(s) 134, 1043, 1052, 1142, 1254
ScrFI CCNGG 4 cut(s) 1008, 1050, 1224, 1285
SduI GDGCHC 2 cut(s) 115, 1015
SfaNI GCATC 2 cut(s) 1009, 1053
SfcI CTRYAG 1 cut(s) 264
SfoI GGCGCC 1 cut(s) 586
SinI GGWCC 2 cut(s) 1052, 1142
SsiI CCGC 6 cut(s) 20, 137, 140, 157, 299, 1133
SspDI GGCGCC 1 cut(s) 584
SspI AATATT 1 cut(s) 220
SspMI CTAG 2 cut(s) 285, 1100
StyD4I CCNGG 4 cut(s) 1006, 1048, 1222, 1283
StyI CCWWGG 2 cut(s) 574, 618
TaaI ACNGT 4 cut(s) 106, 387, 494, 679
TaiI ACGT 3 cut(s) 281, 539, 1001
TaqI TCGA 1 cut(s) 963
TauI GCSGC 4 cut(s) 139, 142, 160, 302
TfiI GAWTC 4 cut(s) 162, 606, 731, 956
Tru1I TTAA 4 cut(s) 32, 638, 770, 1306
Tru9I TTAA 4 cut(s) 32, 638, 770, 1306
TscAI CASTG 2 cut(s) 392, 521
TseFI GTSAC 1 cut(s) 1036
TseI GCWGC 6 cut(s) 231, 266, 296, 311, 314, 722
Tsp45I GTSAC 1 cut(s) 1036
TspDTI ATGAA 2 cut(s) 257, 803
TspGWI ACGGA 1 cut(s) 949
TspRI CASTG 2 cut(s) 392, 521
Tth111I GACNNNGTC 1 cut(s) 538
Van91I CCANNNNNTGG 1 cut(s) 1229
VpaK11BI GGWCC 2 cut(s) 1052, 1142
XapI RAATTY 2 cut(s) 245, 1080
XmnI GAANNNNTTC 2 cut(s) 663, 880
XspI CTAG 2 cut(s) 285, 1100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.