Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr8

Orthogroup ID

OG0016666

Physical Location & Seq

Genomic Coordinates Forward (+)

1345699 .. 1348186

Gene Structure 2488 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1335 bp

ATGAACCCCCCCGTCTTTCTCTTTCTCCTCCCAACTCTCCTCCTCCTCTCCCGCACCTGCCTCTCTTCGCCGCCGCAAACCGCCGCCTCCGAGCTCTTCGAAGCTTGGTGCAAACAGTATGGCAAGTCCTACCCCTCACCGCAAGAAAAGCTCCACAGGCTCAGTGTCTTCGAGGGCAATCTCAACTTTGTCACCCGACACAACGAAATGGGGAATTCTTCTTATACCCTTTCTCTCAATGCGTTTTCTGATCTCACCCACAGCGAGTTCAAGGCCTCCCGATTAGGCTTCTCGCCCTCGTTCATCGGTCTTAAGCGTGAACCGGATAGAAAACCCGGTTTATTGGCTCGTGATGTTCCTTCTTCGATTGATTGGAGGGAGGAAGGGGCGGTGACGAAGGTTAAGGATCAAGGGAGTTGTGGTGCTTGTTGGGCATTCTCAACTACTGGTGCCATCGAAGGTATCAACAAAATCGTCACAGGATCACTTCTCAGCCTTTCCGAACAAGAGCTGATTGATTGCGACAGAGTTTATCCAAACAGCGGCTGCAGTGGTGGACTTATGGACGATGCATTCGAATTTGTCATAGAAAACAATGGGGTCGACACTGAGGAGGATTACCCGTATAAAGGTTTGGATGGCACCTGCAATAAGAAAAAGTTGAAGAGGCATGCTGTGACCATAGATGATTATACTGATGTGCCCCCAAATGATGAGGAACAACTACTACAAGCTGTGGCAAATCAGCCTGTGAGCGTCGGCATATGTGGCAGCGACATTGCATTTCAGTTGTACTCGAAGGGAATTTTCACTGGTCCATGTTCGAATTCTTTGGATCACGCTGTTCTAATTGTAGGTTATGGTTCGGAAAATGGGGTGGACTATTGGATTGTGAAGAACTCATGGGGAAAAAGTTGGGGAATGAATGGCTACATCCACATGCTACGCGATCATAGCAATTCCCAAGGGGTCTGTGGCATCAACAGTTTAGCTTCATATCCCATAAAGACCGGGCAAAACCCTCCTCTTCCCCCGCCTTCTCCTCCCCCAGGTCCAACCAAATGCGACCTCTTTACGCACTGCGCAGCAGGGGAAACCTGCTGTTGTGCCAAGATGTTTATTGGAATATGCTTTGCTTGGAGATGCTGTGAGTTGAACTCCGCTGTTTGTTGCAAGGATAACAGTCACTGTTGTCCCCATGATTATCCCATCTGTGACACAGAAAGGGCGCTCTGCCTCAAGAGTAGCGAACCATCAACGCAATCCCAGGTCACTTCGAACGTAACAACAAAAGCGCTTATGAGCAGAAGCGTTTTGAGGAAGTCGAGCTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009987 GO:0016787 GO:0019538 GO:0030163 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0051603 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418

Pfam Domains

Protein Families

Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

445

Amino Acids

48.56

Weight (kDa)

5.73

Isoelectric Point (pI)

40.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I29	PF08246	33 - 90	4.9e-21	Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1	PF00112	119 - 335	6.6e-84	Papain family cysteine protease
Granulin	PF00396	366 - 413	8.2e-09	Granulin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0016666)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09850
fragaria_vesca	FvH4_2g38850
malus_domestica	MD08G1023300.v1.1
prunus_persica	Prupe.1G372500_v2.0.a1
pyrus_communis	pycom08g01810
rosa_chinensis	RchiOBHm_Chr6g0301951
rosa_laevigata	RLG00000011206
rosa_multiflora	Rmu_sc0000407.1_g000001
rosa_roxburghii	Rroxscaffold_7G00166250
rosa_rugosa	Rorug06G0310500
rosa_samantha	Rh6AG422900 Rh6BG429700 Rh6CG436100
rosa_wichuraiana	Rw6G036630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	65, 653
Acc16I	TGCGCA	1 cut(s)	1084
Acc36I	ACCTGC	3 cut(s)	65, 653, 1106
AccB1I	GGYRCC	2 cut(s)	449, 641
AccI	GTMKAC	1 cut(s)	603
AccII	CGCG	1 cut(s)	948
AclWI	GGATC	3 cut(s)	414, 490, 843
AcsI	RAATTY	4 cut(s)	214, 578, 804, 826
AfaI	GTAC	1 cut(s)	794
AfeI	AGCGCT	1 cut(s)	1296
AfiI	CCNNNNNNNGG	3 cut(s)	542, 629, 1049
AflII	CTTAAG	1 cut(s)	311
AgsI	TTSAA	3 cut(s)	271, 664, 1156
AjnI	CCWGG	2 cut(s)	1048, 1266
AluBI	AGCT	7 cut(s)	94, 104, 151, 511, 734, 992, 1329
AluI	AGCT	7 cut(s)	94, 104, 151, 511, 734, 992, 1329
Alw21I	GWGCWC	1 cut(s)	96
AlwI	GGATC	3 cut(s)	414, 490, 843
AlwNI	CAGNNNCTG	2 cut(s)	546, 1188
Aor51HI	AGCGCT	1 cut(s)	1296
AoxI	GGCC	1 cut(s)	273
ApeKI	GCWGC	3 cut(s)	546, 771, 1085
ApoI	RAATTY	4 cut(s)	214, 578, 804, 826
ArsI	GACNNNNNNTTYG	2 cut(s)	557, 589
AspLEI	GCGC	3 cut(s)	1085, 1231, 1297
AspS9I	GGNCC	2 cut(s)	815, 1052
AsuC2I	CCSGG	2 cut(s)	336, 1012
AsuHPI	GGTGA	4 cut(s)	129, 184, 247, 403
AsuII	TTCGAA	4 cut(s)	99, 576, 824, 1277
AvaII	GGWCC	2 cut(s)	815, 1052
BaeGI	GKGCMC	1 cut(s)	705
BanI	GGYRCC	2 cut(s)	449, 641
BanII	GRGCYC	1 cut(s)	96
BauI	CACGAG	1 cut(s)	348
BbsI	GAAGAC	1 cut(s)	160
Bbv12I	GWGCWC	1 cut(s)	96
BbvI	GCAGC	3 cut(s)	533, 783, 1097
BccI	CCATC	4 cut(s)	461, 632, 1217, 1261
BciT130I	CCWGG	2 cut(s)	1050, 1268
BcnI	CCSGG	2 cut(s)	336, 1012
BfmI	CTRYAG	1 cut(s)	547
BfoI	RGCGCY	2 cut(s)	1232, 1298
BfrI	CTTAAG	1 cut(s)	311
BfuAI	ACCTGC	3 cut(s)	65, 653, 1106
BisI	GCNGC	7 cut(s)	71, 74, 84, 544, 547, 772, 1086
BlsI	GCNGC	7 cut(s)	72, 75, 85, 545, 548, 773, 1087
Bme1390I	CCNGG	4 cut(s)	336, 1012, 1050, 1268
Bme18I	GGWCC	2 cut(s)	815, 1052
BmgT120I	GGNCC	2 cut(s)	815, 1052
BmiI	GGNNCC	2 cut(s)	451, 643
BmrFI	CCNGG	4 cut(s)	336, 1012, 1050, 1268
BmsI	GCATC	3 cut(s)	559, 987, 1133
BpiI	GAAGAC	1 cut(s)	160
BplI	GAGNNNNNCTC	2 cut(s)	1142, 1174
Bpu14I	TTCGAA	4 cut(s)	99, 576, 824, 1277
BpuEI	CTTGAG	1 cut(s)	1223
BpuMI	CCSGG	2 cut(s)	336, 1012
BsaJI	CCNNGG	3 cut(s)	964, 1048, 1266
BsaWI	WCCGGW	1 cut(s)	322
BsaXI	ACNNNNNCTCC	4 cut(s)	406, 436, 1132, 1162
Bsc4I	CCNNNNNNNGG	3 cut(s)	542, 629, 1049
Bse1I	ACTGG	2 cut(s)	451, 817
Bse3DI	GCAATG	1 cut(s)	777
BseBI	CCWGG	2 cut(s)	1050, 1268
BseDI	CCNNGG	3 cut(s)	964, 1048, 1266
BseGI	GGATG	2 cut(s)	643, 933
BseLI	CCNNNNNNNGG	3 cut(s)	542, 629, 1049
BseMI	GCAATG	1 cut(s)	777
BseMII	CTCAG	3 cut(s)	175, 505, 600
BseNI	ACTGG	2 cut(s)	451, 817
BseRI	GAGGAG	7 cut(s)	17, 29, 32, 35, 626, 1014, 1032
BseSI	GKGCMC	1 cut(s)	705
BseXI	GCAGC	3 cut(s)	533, 783, 1097
Bsh1236I	CGCG	1 cut(s)	948
BshFI	GGCC	1 cut(s)	275
BshNI	GGYRCC	2 cut(s)	449, 641
BsiHKAI	GWGCWC	1 cut(s)	96
BsiSI	CCGG	3 cut(s)	323, 336, 1011
BslFI	GGGAC	1 cut(s)	1179
BslI	CCNNNNNNNGG	3 cut(s)	542, 629, 1049
BsmFI	GGGAC	1 cut(s)	1179
BsmI	GAATGC	2 cut(s)	434, 572
BsnI	GGCC	1 cut(s)	275
Bsp119I	TTCGAA	4 cut(s)	99, 576, 824, 1277
Bsp1286I	GDGCHC	2 cut(s)	96, 705
Bsp143I	GATC	5 cut(s)	250, 406, 482, 835, 949
BspANI	GGCC	1 cut(s)	275
BspCNI	CTCAG	3 cut(s)	174, 504, 601
BspFNI	CGCG	1 cut(s)	948
BspLI	GGNNCC	2 cut(s)	451, 643
BspMAI	CTGCAG	1 cut(s)	551
BspMI	ACCTGC	3 cut(s)	65, 653, 1106
BspPI	GGATC	3 cut(s)	414, 490, 843
BspQI	GCTCTTC	1 cut(s)	101
BspT104I	TTCGAA	4 cut(s)	99, 576, 824, 1277
BspT107I	GGYRCC	2 cut(s)	449, 641
BspTI	CTTAAG	1 cut(s)	311
BsrDI	GCAATG	1 cut(s)	777
BsrI	ACTGG	2 cut(s)	451, 817
BssECI	CCNNGG	3 cut(s)	964, 1048, 1266
BssMI	GATC	5 cut(s)	250, 406, 482, 835, 949
BssSI	CACGAG	1 cut(s)	348
BssT1I	CCWWGG	1 cut(s)	964
Bst2BI	CACGAG	1 cut(s)	348
Bst2UI	CCWGG	2 cut(s)	1050, 1268
Bst4CI	ACNGT	4 cut(s)	117, 986, 1184, 1190
Bst6I	CTCTTC	4 cut(s)	70, 101, 659, 1032
BstAFI	CTTAAG	1 cut(s)	311
BstBI	TTCGAA	4 cut(s)	99, 576, 824, 1277
BstC8I	GCNNGC	1 cut(s)	672
BstDEI	CTNAG	3 cut(s)	161, 491, 609
BstENI	CCTNNNNNAGG	1 cut(s)	1047
BstF5I	GGATG	2 cut(s)	643, 933
BstFNI	CGCG	1 cut(s)	948
BstH2I	RGCGCY	2 cut(s)	1232, 1298
BstHHI	GCGC	3 cut(s)	1085, 1231, 1297
BstKTI	GATC	5 cut(s)	253, 409, 485, 838, 952
BstMBI	GATC	5 cut(s)	250, 406, 482, 835, 949
BstMWI	GCNNNNNNNGC	5 cut(s)	148, 157, 431, 768, 954
BstNI	CCWGG	2 cut(s)	1050, 1268
BstNSI	RCATGY	2 cut(s)	674, 943
BstSCI	CCNGG	4 cut(s)	334, 1010, 1048, 1266
BstSFI	CTRYAG	1 cut(s)	547
BstSLI	GKGCMC	1 cut(s)	705
BstUI	CGCG	1 cut(s)	948
BstV1I	GCAGC	3 cut(s)	533, 783, 1097
BstV2I	GAAGAC	1 cut(s)	160
BsuRI	GGCC	1 cut(s)	275
BtsCI	GGATG	2 cut(s)	643, 933
BtsI	GCAGTG	2 cut(s)	556, 1078
BtsIMutI	CAGTG	6 cut(s)	169, 556, 606, 810, 1078, 1186
BveI	ACCTGC	3 cut(s)	65, 653, 1106
Cac8I	GCNNGC	1 cut(s)	672
CaiI	CAGNNNCTG	2 cut(s)	546, 1188
CfoI	GCGC	3 cut(s)	1085, 1231, 1297
Cfr13I	GGNCC	2 cut(s)	815, 1052
CseI	GACGC	1 cut(s)	745
Csp6I	GTAC	1 cut(s)	793
CviAII	CATG	5 cut(s)	671, 819, 903, 940, 1199
CviQI	GTAC	1 cut(s)	793
DdeI	CTNAG	3 cut(s)	161, 491, 609
DpnI	GATC	5 cut(s)	252, 408, 484, 837, 951
DpnII	GATC	5 cut(s)	250, 406, 482, 835, 949
Eam1104I	CTCTTC	4 cut(s)	70, 101, 659, 1032
EarI	CTCTTC	4 cut(s)	70, 101, 659, 1032
Ecl136II	GAGCTC	1 cut(s)	94
Eco130I	CCWWGG	1 cut(s)	964
Eco147I	AGGCCT	1 cut(s)	275
Eco24I	GRGCYC	1 cut(s)	96
Eco47I	GGWCC	2 cut(s)	815, 1052
Eco47III	AGCGCT	1 cut(s)	1296
Eco53kI	GAGCTC	1 cut(s)	94
EcoICRI	GAGCTC	1 cut(s)	94
EcoNI	CCTNNNNNAGG	1 cut(s)	1047
EcoRI	GAATTC	2 cut(s)	214, 826
EcoRII	CCWGG	2 cut(s)	1048, 1266
EcoT14I	CCWWGG	1 cut(s)	964
EcoT22I	ATGCAT	1 cut(s)	574
EcoT38I	GRGCYC	1 cut(s)	96
ErhI	CCWWGG	1 cut(s)	964
FaeI	CATG	5 cut(s)	674, 822, 906, 943, 1202
FaqI	GGGAC	1 cut(s)	1179
FatI	CATG	5 cut(s)	670, 818, 902, 939, 1198
FauI	CCCGC	2 cut(s)	59, 1041
FauNDI	CATATG	1 cut(s)	764
FblI	GTMKAC	1 cut(s)	603
Fnu4HI	GCNGC	7 cut(s)	71, 74, 84, 544, 547, 772, 1086
FokI	GGATG	2 cut(s)	650, 920
FriOI	GRGCYC	1 cut(s)	96
Fsp4HI	GCNGC	7 cut(s)	71, 74, 84, 544, 547, 772, 1086
FspI	TGCGCA	1 cut(s)	1084
GlaI	GCGC	3 cut(s)	1084, 1230, 1296
GluI	GCNGC	7 cut(s)	71, 74, 84, 544, 547, 772, 1086
HaeII	RGCGCY	2 cut(s)	1232, 1298
HaeIII	GGCC	1 cut(s)	275
HapII	CCGG	3 cut(s)	323, 336, 1011
HgaI	GACGC	1 cut(s)	745
HhaI	GCGC	3 cut(s)	1085, 1231, 1297
Hin1II	CATG	5 cut(s)	674, 822, 906, 943, 1202
Hin6I	GCGC	3 cut(s)	1083, 1229, 1295
HinP1I	GCGC	3 cut(s)	1083, 1229, 1295
HincII	GTYRAC	1 cut(s)	604
HindII	GTYRAC	1 cut(s)	604
HindIII	AAGCTT	1 cut(s)	102
HpaII	CCGG	3 cut(s)	323, 336, 1011
HphI	GGTGA	4 cut(s)	129, 184, 247, 403
Hpy166II	GTNNAC	4 cut(s)	320, 557, 604, 880
Hpy188I	TCNGA	4 cut(s)	91, 250, 502, 868
Hpy188III	TCNNGA	3 cut(s)	279, 350, 1240
Hpy8I	GTNNAC	4 cut(s)	320, 557, 604, 880
Hpy99I	CGWCG	1 cut(s)	761
HpyAV	CCTTC	6 cut(s)	369, 377, 391, 452, 793, 1047
HpyCH4III	ACNGT	4 cut(s)	117, 986, 1184, 1190
HpyCH4IV	ACGT	1 cut(s)	1281
HpyCH4V	TGCA	6 cut(s)	111, 549, 572, 648, 782, 1173
HpyF10VI	GCNNNNNNNGC	5 cut(s)	148, 157, 431, 768, 954
HpyF3I	CTNAG	3 cut(s)	161, 491, 609
HpySE526I	ACGT	1 cut(s)	1281
Hsp92II	CATG	5 cut(s)	674, 822, 906, 943, 1202
HspAI	GCGC	3 cut(s)	1083, 1229, 1295
Kzo9I	GATC	5 cut(s)	250, 406, 482, 835, 949
LguI	GCTCTTC	1 cut(s)	101
LmnI	GCTCC	1 cut(s)	156
Lsp1109I	GCAGC	3 cut(s)	533, 783, 1097
LweI	GCATC	3 cut(s)	559, 987, 1133
MaeII	ACGT	1 cut(s)	1281
MaeIII	GTNAC	8 cut(s)	190, 391, 475, 676, 1184, 1214, 1270, 1282
MalI	GATC	5 cut(s)	252, 408, 484, 837, 951
MboI	GATC	5 cut(s)	250, 406, 482, 835, 949
MboII	GAAGA	8 cut(s)	57, 88, 160, 210, 354, 676, 907, 1019
MhlI	GDGCHC	2 cut(s)	96, 705
MluCI	AATT	6 cut(s)	214, 578, 804, 826, 849, 958
MmeI	TCCRAC	1 cut(s)	1079
Mph1103I	ATGCAT	1 cut(s)	574
MseI	TTAA	2 cut(s)	312, 402
MslI	CAYNNNNRTG	1 cut(s)	938
MspA1I	CMGCKG	2 cut(s)	543, 1163
MspCI	CTTAAG	1 cut(s)	311
MspI	CCGG	3 cut(s)	323, 336, 1011
MspR9I	CCNGG	4 cut(s)	336, 1012, 1050, 1268
Mva1269I	GAATGC	2 cut(s)	434, 572
MvaI	CCWGG	2 cut(s)	1050, 1268
MvnI	CGCG	1 cut(s)	948
MwoI	GCNNNNNNNGC	5 cut(s)	148, 157, 431, 768, 954
NciI	CCSGG	2 cut(s)	336, 1012
NdeI	CATATG	1 cut(s)	764
NdeII	GATC	5 cut(s)	250, 406, 482, 835, 949
NlaIII	CATG	5 cut(s)	674, 822, 906, 943, 1202
NlaIV	GGNNCC	2 cut(s)	451, 643
NmuCI	GTSAC	7 cut(s)	190, 391, 475, 676, 1184, 1214, 1270
NsbI	TGCGCA	1 cut(s)	1084
NsiI	ATGCAT	1 cut(s)	574
NspI	RCATGY	2 cut(s)	674, 943
NspV	TTCGAA	4 cut(s)	99, 576, 824, 1277
PaeI	GCATGC	1 cut(s)	674
PaqCI	CACCTGC	2 cut(s)	65, 653
PceI	AGGCCT	1 cut(s)	275
PciSI	GCTCTTC	1 cut(s)	101
PcsI	WCGNNNNNNNCGW	1 cut(s)	573
PctI	GAATGC	2 cut(s)	434, 572
PkrI	GCNGC	7 cut(s)	72, 75, 85, 545, 548, 773, 1087
Psp124BI	GAGCTC	1 cut(s)	96
Psp6I	CCWGG	2 cut(s)	1048, 1266
PspGI	CCWGG	2 cut(s)	1048, 1266
PspN4I	GGNNCC	2 cut(s)	451, 643
PspPI	GGNCC	2 cut(s)	815, 1052
PsrI	GAACNNNNNNTAC	2 cut(s)	711, 743
PstI	CTGCAG	1 cut(s)	551
PstNI	CAGNNNCTG	2 cut(s)	546, 1188
RsaI	GTAC	1 cut(s)	794
RsaNI	GTAC	1 cut(s)	793
RseI	CAYNNNNRTG	1 cut(s)	938
SacI	GAGCTC	1 cut(s)	96
SalI	GTCGAC	1 cut(s)	602
SapI	GCTCTTC	1 cut(s)	101
SaqAI	TTAA	2 cut(s)	312, 402
SatI	GCNGC	7 cut(s)	71, 74, 84, 544, 547, 772, 1086
Sau3AI	GATC	5 cut(s)	250, 406, 482, 835, 949
Sau96I	GGNCC	2 cut(s)	815, 1052
ScrFI	CCNGG	4 cut(s)	336, 1012, 1050, 1268
SduI	GDGCHC	2 cut(s)	96, 705
SfaNI	GCATC	3 cut(s)	559, 987, 1133
SfcI	CTRYAG	1 cut(s)	547
SfuI	TTCGAA	4 cut(s)	99, 576, 824, 1277
SinI	GGWCC	2 cut(s)	815, 1052
SmiMI	CAYNNNNRTG	1 cut(s)	938
SmlI	CTYRAG	2 cut(s)	311, 1238
SmoI	CTYRAG	2 cut(s)	311, 1238
SphI	GCATGC	1 cut(s)	674
Sse9I	AATT	6 cut(s)	214, 578, 804, 826, 849, 958
SseBI	AGGCCT	1 cut(s)	275
SstI	GAGCTC	1 cut(s)	96
StuI	AGGCCT	1 cut(s)	275
StyD4I	CCNGG	4 cut(s)	334, 1010, 1048, 1266
StyI	CCWWGG	1 cut(s)	964
TaaI	ACNGT	4 cut(s)	117, 986, 1184, 1190
TaiI	ACGT	1 cut(s)	1284
TaqII	GACCGA	1 cut(s)	296
TasI	AATT	6 cut(s)	214, 578, 804, 826, 849, 958
TatI	WGTACW	1 cut(s)	792
TauI	GCSGC	4 cut(s)	73, 76, 86, 546
Tru1I	TTAA	2 cut(s)	312, 402
Tru9I	TTAA	2 cut(s)	312, 402
TscAI	CASTG	6 cut(s)	169, 556, 613, 817, 1085, 1193
TseFI	GTSAC	7 cut(s)	190, 391, 475, 676, 1184, 1214, 1270
TseI	GCWGC	3 cut(s)	546, 771, 1085
Tsp45I	GTSAC	7 cut(s)	190, 391, 475, 676, 1184, 1214, 1270
TspDTI	ATGAA	4 cut(s)	17, 292, 938, 984
TspRI	CASTG	6 cut(s)	169, 556, 613, 817, 1085, 1193
Vha464I	CTTAAG	1 cut(s)	311
VpaK11BI	GGWCC	2 cut(s)	815, 1052
XagI	CCTNNNNNAGG	1 cut(s)	1047
XapI	RAATTY	4 cut(s)	214, 578, 804, 826
XceI	RCATGY	2 cut(s)	674, 943
XcmI	CCANNNNNNNNNTGG	1 cut(s)	971
XmiI	GTMKAC	1 cut(s)	603
Zsp2I	ATGCAT	1 cut(s)	574

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.