pycom08g04120

protein At1g32220, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012254
Physical Location & Seq
Reverse (-)
3161467 .. 3164493
3027 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1074 bp
ATGACATGTGTACACGTTGCGTTTTCCCTAAGGCCTTGCACGAACCACTTGTCTCCACAGTTACGCGCCATCTGTTCCTTCTTGTCTGTAAAACAAGAAGCTACAGACTTTCCGGTTACTTGCGCTCCGGGCATTCTCCGAGAAAGCAAATTGAAGAACCAAAATTTAAGAAACCCTAGAAAAAAGGGGACTGTGTGTAACCGCGAATTCGAAAAAATGAGGACGGTCGTCTCGCGGTTGATCCATACAAGATCATCACTTCCCAGGCTCTTTGTATCGGATGCACCGAGGAATGGTAGGTATCTGACAACAGACTCTGATAAGGTTGATGAACCCTTCAAAGTTGAGGAAGCAGAGACTGTAAGCACTCCCCCTCCGCCAACTGAGAAGTTGCTTGTCTTGGGTGGAAATGGATTTGTTGGCTCACATGTTTGTAGAGAAGCATTGGAGCGGGGCTTGTCTGTCGCTAGCCTTAGCAGGTCTGGTAGGTCAAAATTACATGATTCATGGGCCGACAGTATGACCTGGCATCAAGGAAACCTTCTATCGCCCGAGTCATGGAAGGATGCCCTCGATGGTGTCACGTCTGTGATATCCTGTGTTGGTGGGTTTGGCTCCAATTCCTACATGTATAAGATTAATGGGACTGCTAACATCAATGCAATTAGAGCTGCCGCTGAACAAGGTGTGAAAAGATTTGTTTATATCTCTGCTGCTGATTTTGGCGTGGCAAATTACTTATTGCGGGGATATTATGAGGGAAAGAGAGCTGCAGAGACAGAGCTGCTAACCAAATTTCCTTACGGAGGAGTGATCTTGAGGCCTGGATTTATTTATGGGACTCGAAGTGTTGGCAGTGTAAAGTTACCTCTCGGTGTAATTGGTTATCCACTTGAGATGCTATTTCAATATACAAGACCTCTGAGCCAGCTTCCGCTTGTTGGGCCTCTCTTTACTCCTCCAGTGAATGTCACCTCCGTGGCAAATGTTGCCGTTAGAGCAGCTACTGATCCCGTCTTCCCTCCTGGGATCGTAGATGTCCAAGGGATCCAGCGTTATACCCAGAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

358

Amino Acids

39.14

Weight (kDa)

9.53

Isoelectric Point (pI)

37.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 128 - 237 9.1e-10 NmrA-like family
Epimerase PF01370 132 - 239 9.4e-15 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 132 - 245 1.4e-09 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 135 - 278 6.5e-19 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012254)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10730
fragaria_vesca FvH4_2g41440 FvH4_2g41440
malus_domestica MD08G1049300.v1.1 MD15G1045600.v1.1
prunus_persica Prupe.1G399300_v2.0.a1
pyrus_communis pycom08g04120 pycom15g04250
rosa_chinensis RchiOBHm_Chr6g0299041
rosa_laevigata RLG00000011467
rosa_multiflora Rmu_sc0000981.1_g000004 Rmu_sc0032629.1_g000004
rosa_roxburghii Rroxscaffold_7G00169060
rosa_rugosa Rorug06G0284300
rosa_samantha Rh6AG395800 Rh6BG404400 Rh6CG410300 Rh6DG397000
rosa_wichuraiana Rw6G034640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 468
AccBSI CCGCTC 1 cut(s) 451
AccII CGCG 3 cut(s) 66, 204, 235
AciI CCGC 7 cut(s) 202, 235, 377, 451, 675, 745, 935
AclWI GGATC 5 cut(s) 235, 1004, 1037, 1042, 1055
AcsI RAATTY 3 cut(s) 163, 206, 794
AfaI GTAC 1 cut(s) 12
AfiI CCNNNNNNNGG 4 cut(s) 293, 558, 806, 941
AflIII ACRYGT 4 cut(s) 5, 13, 427, 627
AgsI TTSAA 3 cut(s) 154, 340, 908
AjiI CACGTC 1 cut(s) 585
AjnI CCWGG 4 cut(s) 263, 524, 823, 1024
AleI CACNNNNGTG 2 cut(s) 587, 977
AluBI AGCT 6 cut(s) 101, 671, 770, 784, 931, 1004
AluI AGCT 6 cut(s) 101, 671, 770, 784, 931, 1004
Alw26I GTCTC 4 cut(s) 57, 235, 350, 770
AlwI GGATC 5 cut(s) 235, 1004, 1037, 1042, 1055
AlwNI CAGNNNCTG 3 cut(s) 317, 359, 1007
Ama87I CYCGRG 1 cut(s) 551
AoxI GGCC 4 cut(s) 32, 510, 821, 944
ApeKI GCWGC 5 cut(s) 671, 713, 770, 784, 1001
ApoI RAATTY 3 cut(s) 163, 206, 794
ArsI GACNNNNNNTTYG 2 cut(s) 35, 67
AseI ATTAAT 1 cut(s) 639
AspLEI GCGC 2 cut(s) 68, 125
AspS9I GGNCC 2 cut(s) 510, 944
AsuC2I CCSGG 1 cut(s) 129
AsuHPI GGTGA 1 cut(s) 964
AsuII TTCGAA 1 cut(s) 210
AsuNHI GCTAGC 1 cut(s) 467
AvaI CYCGRG 1 cut(s) 551
AxyI CCTNAGG 1 cut(s) 29
BamHI GGATCC 1 cut(s) 1047
BbsI GAAGAC 1 cut(s) 1009
BbvI GCAGC 5 cut(s) 658, 700, 757, 771, 1013
BccI CCATC 2 cut(s) 77, 569
BceAI ACGGC 1 cut(s) 977
BciT130I CCWGG 4 cut(s) 265, 526, 825, 1026
BcnI CCSGG 1 cut(s) 129
BcoDI GTCTC 4 cut(s) 57, 235, 350, 770
BfaI CTAG 2 cut(s) 177, 468
BfmI CTRYAG 2 cut(s) 102, 771
BfuAI ACCTGC 1 cut(s) 468
BisI GCNGC 6 cut(s) 672, 675, 714, 771, 785, 1002
BlsI GCNGC 6 cut(s) 673, 676, 715, 772, 786, 1003
Bme1390I CCNGG 5 cut(s) 129, 265, 526, 825, 1026
BmeT110I CYCGRG 1 cut(s) 551
BmgBI CACGTC 1 cut(s) 585
BmgT120I GGNCC 2 cut(s) 510, 944
BmiI GGNNCC 2 cut(s) 616, 1049
BmrFI CCNGG 5 cut(s) 129, 265, 526, 825, 1026
BmsI GCATC 4 cut(s) 271, 538, 556, 888
BmtI GCTAGC 1 cut(s) 471
BoxI GACNNNNGTC 1 cut(s) 227
BpiI GAAGAC 1 cut(s) 1009
BpmI CTGGAG 1 cut(s) 945
Bpu10I CCTNAGC 1 cut(s) 473
Bpu14I TTCGAA 1 cut(s) 210
BpuEI CTTGAG 2 cut(s) 838, 914
BpuMI CCSGG 1 cut(s) 129
BsaJI CCNNGG 5 cut(s) 263, 287, 978, 1025, 1042
BsaWI WCCGGW 1 cut(s) 112
BsaXI ACNNNNNCTCC 4 cut(s) 109, 139, 358, 388
Bsc4I CCNNNNNNNGG 4 cut(s) 293, 558, 806, 941
Bse1I ACTGG 1 cut(s) 962
Bse21I CCTNAGG 1 cut(s) 29
BseBI CCWGG 4 cut(s) 265, 526, 825, 1026
BseDI CCNNGG 5 cut(s) 263, 287, 978, 1025, 1042
BseGI GGATG 2 cut(s) 286, 571
BseLI CCNNNNNNNGG 4 cut(s) 293, 558, 806, 941
BseMII CTCAG 2 cut(s) 375, 914
BseNI ACTGG 1 cut(s) 962
BseRI GAGGAG 2 cut(s) 822, 948
BseXI GCAGC 5 cut(s) 658, 700, 757, 771, 1013
Bsh1236I CGCG 3 cut(s) 66, 204, 235
Bsh1285I CGRYCG 1 cut(s) 228
BshFI GGCC 4 cut(s) 34, 512, 823, 946
BsiEI CGRYCG 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 551
BsiSI CCGG 2 cut(s) 113, 128
BslFI GGGAC 3 cut(s) 202, 658, 853
BslI CCNNNNNNNGG 4 cut(s) 293, 558, 806, 941
BsmAI GTCTC 4 cut(s) 57, 235, 350, 770
BsmBI CGTCTC 1 cut(s) 235
BsmFI GGGAC 3 cut(s) 202, 658, 853
BsmI GAATGC 1 cut(s) 132
BsnI GGCC 4 cut(s) 34, 512, 823, 946
BsoBI CYCGRG 1 cut(s) 551
Bsp119I TTCGAA 1 cut(s) 210
Bsp1407I TGTACA 1 cut(s) 10
Bsp143I GATC 6 cut(s) 240, 251, 813, 1009, 1029, 1047
BspACI CCGC 7 cut(s) 202, 235, 377, 451, 675, 745, 935
BspANI GGCC 4 cut(s) 34, 512, 823, 946
BspCNI CTCAG 2 cut(s) 376, 915
BspFNI CGCG 3 cut(s) 66, 204, 235
BspLI GGNNCC 2 cut(s) 616, 1049
BspMAI CTGCAG 1 cut(s) 775
BspMI ACCTGC 1 cut(s) 468
BspOI GCTAGC 1 cut(s) 471
BspPI GGATC 5 cut(s) 235, 1004, 1037, 1042, 1055
BspT104I TTCGAA 1 cut(s) 210
BsrBI CCGCTC 1 cut(s) 451
BsrGI TGTACA 1 cut(s) 10
BsrI ACTGG 1 cut(s) 962
BssECI CCNNGG 5 cut(s) 263, 287, 978, 1025, 1042
BssMI GATC 6 cut(s) 240, 251, 813, 1009, 1029, 1047
BssT1I CCWWGG 1 cut(s) 1042
Bst2UI CCWGG 4 cut(s) 265, 526, 825, 1026
Bst4CI ACNGT 5 cut(s) 60, 193, 226, 361, 518
BstAPI GCANNNNNTGC 1 cut(s) 989
BstAUI TGTACA 1 cut(s) 10
BstBI TTCGAA 1 cut(s) 210
BstC8I GCNNGC 2 cut(s) 469, 929
BstDEI CTNAG 4 cut(s) 29, 384, 473, 923
BstDSI CCRYGG 1 cut(s) 978
BstENI CCTNNNNNAGG 1 cut(s) 804
BstF5I GGATG 2 cut(s) 286, 571
BstFNI CGCG 3 cut(s) 66, 204, 235
BstHHI GCGC 2 cut(s) 68, 125
BstKTI GATC 6 cut(s) 243, 254, 816, 1012, 1032, 1050
BstMAI GTCTC 4 cut(s) 57, 235, 350, 770
BstMBI GATC 6 cut(s) 240, 251, 813, 1009, 1029, 1047
BstMCI CGRYCG 1 cut(s) 228
BstMWI GCNNNNNNNGC 5 cut(s) 129, 668, 943, 989, 998
BstNI CCWGG 4 cut(s) 265, 526, 825, 1026
BstNSI RCATGY 3 cut(s) 9, 431, 631
BstPAI GACNNNNGTC 1 cut(s) 227
BstSCI CCNGG 5 cut(s) 127, 263, 524, 823, 1024
BstSFI CTRYAG 2 cut(s) 102, 771
BstUI CGCG 3 cut(s) 66, 204, 235
BstV1I GCAGC 5 cut(s) 658, 700, 757, 771, 1013
BstV2I GAAGAC 1 cut(s) 1009
BstX2I RGATCY 1 cut(s) 1047
BstYI RGATCY 1 cut(s) 1047
Bsu36I CCTNAGG 1 cut(s) 29
BsuRI GGCC 4 cut(s) 34, 512, 823, 946
BtgI CCRYGG 1 cut(s) 978
BtrI CACGTC 1 cut(s) 585
BtsCI GGATG 2 cut(s) 286, 571
BtsI GCAGTG 1 cut(s) 862
BtsIMutI CAGTG 2 cut(s) 862, 969
BveI ACCTGC 1 cut(s) 468
Cac8I GCNNGC 2 cut(s) 469, 929
CaiI CAGNNNCTG 3 cut(s) 317, 359, 1007
CfoI GCGC 2 cut(s) 68, 125
Cfr13I GGNCC 2 cut(s) 510, 944
Csp6I GTAC 1 cut(s) 11
CviAII CATG 6 cut(s) 6, 428, 500, 507, 558, 628
CviQI GTAC 1 cut(s) 11
DdeI CTNAG 4 cut(s) 29, 384, 473, 923
DpnI GATC 6 cut(s) 242, 253, 815, 1011, 1031, 1049
DpnII GATC 6 cut(s) 240, 251, 813, 1009, 1029, 1047
EciI GGCGGA 1 cut(s) 366
Eco130I CCWWGG 1 cut(s) 1042
Eco147I AGGCCT 2 cut(s) 34, 823
Eco32I GATATC 1 cut(s) 594
Eco81I CCTNAGG 1 cut(s) 29
Eco88I CYCGRG 1 cut(s) 551
EcoNI CCTNNNNNAGG 1 cut(s) 804
EcoRI GAATTC 1 cut(s) 206
EcoRII CCWGG 4 cut(s) 263, 524, 823, 1024
EcoRV GATATC 1 cut(s) 594
EcoT14I CCWWGG 1 cut(s) 1042
ErhI CCWWGG 1 cut(s) 1042
Esp3I CGTCTC 1 cut(s) 235
FaeI CATG 6 cut(s) 9, 431, 503, 510, 561, 631
FalI AAGNNNNNCTT 2 cut(s) 525, 557
FaqI GGGAC 3 cut(s) 202, 658, 853
FatI CATG 6 cut(s) 5, 427, 499, 506, 557, 627
FauI CCCGC 2 cut(s) 444, 738
Fnu4HI GCNGC 6 cut(s) 672, 675, 714, 771, 785, 1002
FokI GGATG 2 cut(s) 293, 578
Fsp4HI GCNGC 6 cut(s) 672, 675, 714, 771, 785, 1002
FspBI CTAG 2 cut(s) 177, 468
GlaI GCGC 2 cut(s) 67, 124
GluI GCNGC 6 cut(s) 672, 675, 714, 771, 785, 1002
GsuI CTGGAG 1 cut(s) 945
HaeIII GGCC 4 cut(s) 34, 512, 823, 946
HapII CCGG 2 cut(s) 113, 128
HhaI GCGC 2 cut(s) 68, 125
Hin1II CATG 6 cut(s) 9, 431, 503, 510, 561, 631
Hin6I GCGC 2 cut(s) 66, 123
HinP1I GCGC 2 cut(s) 66, 123
HinfI GANTC 4 cut(s) 314, 503, 554, 841
HpaII CCGG 2 cut(s) 113, 128
HphI GGTGA 1 cut(s) 964
Hpy166II GTNNAC 2 cut(s) 11, 13
Hpy188I TCNGA 5 cut(s) 140, 280, 306, 319, 924
Hpy188III TCNNGA 1 cut(s) 817
Hpy8I GTNNAC 2 cut(s) 11, 13
HpyAV CCTTC 4 cut(s) 88, 346, 551, 556
HpyCH4III ACNGT 5 cut(s) 60, 193, 226, 361, 518
HpyCH4IV ACGT 2 cut(s) 15, 584
HpyCH4V TGCA 4 cut(s) 39, 284, 662, 773
HpyF10VI GCNNNNNNNGC 5 cut(s) 129, 668, 943, 989, 998
HpyF3I CTNAG 4 cut(s) 29, 384, 473, 923
HpySE526I ACGT 2 cut(s) 15, 584
Hsp92II CATG 6 cut(s) 9, 431, 503, 510, 561, 631
HspAI GCGC 2 cut(s) 66, 123
Kzo9I GATC 6 cut(s) 240, 251, 813, 1009, 1029, 1047
LmnI GCTCC 3 cut(s) 130, 448, 620
Lsp1109I GCAGC 5 cut(s) 658, 700, 757, 771, 1013
LweI GCATC 4 cut(s) 271, 538, 556, 888
MaeI CTAG 2 cut(s) 177, 468
MaeII ACGT 2 cut(s) 15, 584
MaeIII GTNAC 6 cut(s) 60, 115, 197, 580, 864, 970
MalI GATC 6 cut(s) 242, 253, 815, 1011, 1031, 1049
MbiI CCGCTC 1 cut(s) 451
MboI GATC 6 cut(s) 240, 251, 813, 1009, 1029, 1047
MboII GAAGA 2 cut(s) 166, 1009
MflI RGATCY 1 cut(s) 1047
MluCI AATT 9 cut(s) 149, 163, 206, 494, 619, 663, 733, 794, 879
MlyI GAGTC 3 cut(s) 308, 563, 835
MseI TTAA 2 cut(s) 167, 639
MslI CAYNNNNRTG 2 cut(s) 587, 977
MspA1I CMGCKG 1 cut(s) 677
MspI CCGG 2 cut(s) 113, 128
MspR9I CCNGG 5 cut(s) 129, 265, 526, 825, 1026
Mva1269I GAATGC 1 cut(s) 132
MvaI CCWGG 4 cut(s) 265, 526, 825, 1026
MvnI CGCG 3 cut(s) 66, 204, 235
MwoI GCNNNNNNNGC 5 cut(s) 129, 668, 943, 989, 998
NciI CCSGG 1 cut(s) 129
NdeII GATC 6 cut(s) 240, 251, 813, 1009, 1029, 1047
NheI GCTAGC 1 cut(s) 467
NlaIII CATG 6 cut(s) 9, 431, 503, 510, 561, 631
NlaIV GGNNCC 2 cut(s) 616, 1049
NmuCI GTSAC 2 cut(s) 580, 970
NspI RCATGY 3 cut(s) 9, 431, 631
NspV TTCGAA 1 cut(s) 210
OliI CACNNNNGTG 2 cut(s) 587, 977
PceI AGGCCT 2 cut(s) 34, 823
PciI ACATGT 3 cut(s) 5, 427, 627
PcsI WCGNNNNNNNCGW 1 cut(s) 284
PctI GAATGC 1 cut(s) 132
PfeI GAWTC 1 cut(s) 503
PkrI GCNGC 6 cut(s) 673, 676, 715, 772, 786, 1003
PleI GAGTC 3 cut(s) 308, 562, 835
PpsI GAGTC 3 cut(s) 308, 562, 835
PscI ACATGT 3 cut(s) 5, 427, 627
PshAI GACNNNNGTC 1 cut(s) 227
PshBI ATTAAT 1 cut(s) 639
Psp6I CCWGG 4 cut(s) 263, 524, 823, 1024
PspGI CCWGG 4 cut(s) 263, 524, 823, 1024
PspN4I GGNNCC 2 cut(s) 616, 1049
PspPI GGNCC 2 cut(s) 510, 944
PstI CTGCAG 1 cut(s) 775
PstNI CAGNNNCTG 3 cut(s) 317, 359, 1007
PsuI RGATCY 1 cut(s) 1047
RsaI GTAC 1 cut(s) 12
RsaNI GTAC 1 cut(s) 11
RseI CAYNNNNRTG 2 cut(s) 587, 977
SaqAI TTAA 2 cut(s) 167, 639
SatI GCNGC 6 cut(s) 672, 675, 714, 771, 785, 1002
Sau3AI GATC 6 cut(s) 240, 251, 813, 1009, 1029, 1047
Sau96I GGNCC 2 cut(s) 510, 944
SchI GAGTC 3 cut(s) 308, 563, 835
ScrFI CCNGG 5 cut(s) 129, 265, 526, 825, 1026
SfaNI GCATC 4 cut(s) 271, 538, 556, 888
SfcI CTRYAG 2 cut(s) 102, 771
SfuI TTCGAA 1 cut(s) 210
SmiMI CAYNNNNRTG 2 cut(s) 587, 977
SmlI CTYRAG 2 cut(s) 817, 893
SmoI CTYRAG 2 cut(s) 817, 893
Sse9I AATT 9 cut(s) 149, 163, 206, 494, 619, 663, 733, 794, 879
SseBI AGGCCT 2 cut(s) 34, 823
SsiI CCGC 7 cut(s) 202, 235, 377, 451, 675, 745, 935
SspMI CTAG 2 cut(s) 177, 468
StuI AGGCCT 2 cut(s) 34, 823
StyD4I CCNGG 5 cut(s) 127, 263, 524, 823, 1024
StyI CCWWGG 1 cut(s) 1042
TaaI ACNGT 5 cut(s) 60, 193, 226, 361, 518
TaiI ACGT 2 cut(s) 18, 587
TaqI TCGA 3 cut(s) 210, 573, 844
TasI AATT 9 cut(s) 149, 163, 206, 494, 619, 663, 733, 794, 879
TatI WGTACW 1 cut(s) 10
TauI GCSGC 1 cut(s) 677
TfiI GAWTC 1 cut(s) 503
Tru1I TTAA 2 cut(s) 167, 639
Tru9I TTAA 2 cut(s) 167, 639
TscAI CASTG 2 cut(s) 862, 969
TseFI GTSAC 2 cut(s) 580, 970
TseI GCWGC 5 cut(s) 671, 713, 770, 784, 1001
Tsp45I GTSAC 2 cut(s) 580, 970
TspDTI ATGAA 2 cut(s) 345, 495
TspGWI ACGGA 2 cut(s) 819, 967
TspRI CASTG 2 cut(s) 862, 969
VspI ATTAAT 1 cut(s) 639
XagI CCTNNNNNAGG 1 cut(s) 804
XapI RAATTY 3 cut(s) 163, 206, 794
XceI RCATGY 3 cut(s) 9, 431, 631
XspI CTAG 2 cut(s) 177, 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.