pycom08g06240

Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008582
Physical Location & Seq
Forward (+)
4845847 .. 4847422
1576 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 957 bp
ATGTACATTATGAAATCTTCCAACGACGGGAAATTTGAACAAGGCCAACTTAGTCGTTATGGAAATATCGAACTTAACCCTGCTGCAGCAGTTTTAAATTATGGACAGGCAATATATGAAGGCACAAAAGCATACAGGAAGAGAGATGGGAAACTCCTCCTCTTTCGTCTAGACCAGAACGCGATTCGGATGAAGATTAGCGCGGAAAGAATGTGCATGCCTTCACTGTCAGTTGATCAATTCATTGATGCTGTAAAGCAAACAGCTCAAGCAAACAAGTGTTGGGTTCCTCCTCCAGGCAAAGGGTCTTTGTATATTAGGCTTCTACTTATAGGAAATGGCCCCATATTAGGCTTGGCACCATCACCTGAGTACACATTCCTTGTATATGCTTCCCCGGTCGGAAATTATTTCAAGGAGGGCTCTGCACCCTTGAACATATTTGTCGAGGAGGAGTATGATCGTGCCTCTCGTGGCGGAGCTGGAGGTGTCAAAGGCAGTACCAATTATGCCCCGGTTTTGAAGGCACTAACTAGAGCAAAAAGCAGGGGATTTTCGGATGTTCTATATCTTGATTCGGTACACAACAAAAATCTGGAGGAGGTCTCTTCTTGCAACATTTTCATTGTGAAAGACAATGTAATTTCAACTCCTGCCACGTCGGGGACTATTCTTTCGGGAGTTACTCGAAAAAGCGTTATTGAAATAGCTCGTGATCATGGTTACCAGGTCGAGGAACGCGTTATTCCGGTGGACGAATTGAGTGAAGCCGATGAAGTGTTCTGCACTGGAACTGCTGTTGGTGTTGCTATCGTGGGCAGCATTAGTTACAAAGGCCAAAGGATGGAGTACAAAACTGGTGCTAAGACTGTAAGTCCACAACTTTACTCAACCCTAGTAAGAATTCAAACGGGACAGATTGAGGAAAAGAAAGGCTGGATTATGGAAATTGACTGA

Protein Analysis

319

Amino Acids

34.87

Weight (kDa)

8.49

Isoelectric Point (pI)

43.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_4 PF01063 38 - 275 1.4e-36 Amino-transferase class IV
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 358
AccB7I CCANNNNNTGG 1 cut(s) 844
AccII CGCG 3 cut(s) 182, 203, 741
AciI CCGC 2 cut(s) 203, 477
AcsI RAATTY 2 cut(s) 32, 903
AfaI GTAC 5 cut(s) 5, 374, 502, 582, 851
AfiI CCNNNNNNNGG 7 cut(s) 27, 296, 302, 350, 663, 733, 844
AflIII ACRYGT 1 cut(s) 739
AgsI TTSAA 7 cut(s) 38, 415, 436, 523, 648, 704, 908
AhdI GACNNNNNGTC 1 cut(s) 873
AjiI CACGTC 1 cut(s) 660
AjnI CCWGG 2 cut(s) 295, 726
AluBI AGCT 3 cut(s) 266, 482, 710
AluI AGCT 3 cut(s) 266, 482, 710
Alw26I GTCTC 1 cut(s) 610
AoxI GGCC 3 cut(s) 43, 340, 835
ApeKI GCWGC 3 cut(s) 83, 86, 819
ApoI RAATTY 2 cut(s) 32, 903
ArsI GACNNNNNNTTYG 4 cut(s) 17, 49, 658, 690
AspLEI GCGC 1 cut(s) 203
AspS9I GGNCC 1 cut(s) 341
AsuC2I CCSGG 2 cut(s) 398, 515
AsuHPI GGTGA 1 cut(s) 357
BanI GGYRCC 1 cut(s) 358
BanII GRGCYC 1 cut(s) 425
BauI CACGAG 2 cut(s) 471, 711
BbvI GCAGC 3 cut(s) 70, 98, 831
BccI CCATC 3 cut(s) 140, 370, 838
BciT130I CCWGG 2 cut(s) 297, 728
BclI TGATCA 2 cut(s) 235, 715
BcnI CCSGG 2 cut(s) 398, 515
BcoDI GTCTC 1 cut(s) 610
BfaI CTAG 3 cut(s) 170, 534, 896
BfmI CTRYAG 1 cut(s) 84
BisI GCNGC 3 cut(s) 84, 87, 820
BlsI GCNGC 3 cut(s) 85, 88, 821
Bme1390I CCNGG 4 cut(s) 297, 398, 515, 728
BmeRI GACNNNNNGTC 1 cut(s) 873
BmgBI CACGTC 1 cut(s) 660
BmgT120I GGNCC 1 cut(s) 341
BmiI GGNNCC 3 cut(s) 288, 343, 360
BmrFI CCNGG 4 cut(s) 297, 398, 515, 728
BmsI GCATC 1 cut(s) 238
BplI GAGNNNNNCTC 2 cut(s) 590, 622
BpmI CTGGAG 3 cut(s) 279, 504, 617
BpuEI CTTGAG 1 cut(s) 252
BpuMI CCSGG 2 cut(s) 398, 515
BsaI GGTCTC 1 cut(s) 610
BsaJI CCNNGG 2 cut(s) 396, 513
BsaWI WCCGGW 1 cut(s) 748
Bsc4I CCNNNNNNNGG 7 cut(s) 27, 296, 302, 350, 663, 733, 844
Bse1I ACTGG 2 cut(s) 793, 862
BseBI CCWGG 2 cut(s) 297, 728
BseDI CCNNGG 2 cut(s) 396, 513
BseGI GGATG 3 cut(s) 195, 565, 849
BseLI CCNNNNNNNGG 7 cut(s) 27, 296, 302, 350, 663, 733, 844
BseMII CTCAG 1 cut(s) 360
BseNI ACTGG 2 cut(s) 793, 862
BseRI GAGGAG 6 cut(s) 146, 149, 282, 464, 467, 614
BseXI GCAGC 3 cut(s) 70, 98, 831
BsgI GTGCAG 2 cut(s) 411, 769
Bsh1236I CGCG 3 cut(s) 182, 203, 741
Bsh1285I CGRYCG 1 cut(s) 402
BshFI GGCC 3 cut(s) 45, 342, 837
BshNI GGYRCC 1 cut(s) 358
BsiEI CGRYCG 1 cut(s) 402
BsiSI CCGG 3 cut(s) 398, 515, 749
BslFI GGGAC 2 cut(s) 679, 927
BslI CCNNNNNNNGG 7 cut(s) 27, 296, 302, 350, 663, 733, 844
BsmAI GTCTC 1 cut(s) 610
BsmFI GGGAC 2 cut(s) 679, 927
BsnI GGCC 3 cut(s) 45, 342, 837
Bso31I GGTCTC 1 cut(s) 610
Bsp1286I GDGCHC 1 cut(s) 425
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 3 cut(s) 235, 460, 715
BspACI CCGC 2 cut(s) 203, 477
BspANI GGCC 3 cut(s) 45, 342, 837
BspCNI CTCAG 1 cut(s) 361
BspFNI CGCG 3 cut(s) 182, 203, 741
BspLI GGNNCC 3 cut(s) 288, 343, 360
BspMAI CTGCAG 1 cut(s) 88
BspT107I GGYRCC 1 cut(s) 358
BspTNI GGTCTC 1 cut(s) 610
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 2 cut(s) 793, 862
BssECI CCNNGG 2 cut(s) 396, 513
BssMI GATC 3 cut(s) 235, 460, 715
BssSI CACGAG 2 cut(s) 471, 711
Bst2BI CACGAG 2 cut(s) 471, 711
Bst2UI CCWGG 2 cut(s) 297, 728
Bst4CI ACNGT 2 cut(s) 228, 871
Bst6I CTCTTC 2 cut(s) 134, 613
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 1 cut(s) 218
BstDEI CTNAG 3 cut(s) 50, 369, 864
BstEII GGTNACC 1 cut(s) 722
BstENI CCTNNNNNAGG 1 cut(s) 294
BstF5I GGATG 3 cut(s) 195, 565, 849
BstFNI CGCG 3 cut(s) 182, 203, 741
BstHHI GCGC 1 cut(s) 203
BstKTI GATC 3 cut(s) 238, 463, 718
BstMAI GTCTC 1 cut(s) 610
BstMBI GATC 3 cut(s) 235, 460, 715
BstMCI CGRYCG 1 cut(s) 402
BstNI CCWGG 2 cut(s) 297, 728
BstNSI RCATGY 1 cut(s) 220
BstPI GGTNACC 1 cut(s) 722
BstSCI CCNGG 4 cut(s) 295, 396, 513, 726
BstSFI CTRYAG 1 cut(s) 84
BstUI CGCG 3 cut(s) 182, 203, 741
BstV1I GCAGC 3 cut(s) 70, 98, 831
BsuRI GGCC 3 cut(s) 45, 342, 837
BtrI CACGTC 1 cut(s) 660
BtsCI GGATG 3 cut(s) 195, 565, 849
BtsIMutI CAGTG 2 cut(s) 224, 786
Cac8I GCNNGC 1 cut(s) 218
CfoI GCGC 1 cut(s) 203
Cfr13I GGNCC 1 cut(s) 341
CsiI ACCWGGT 1 cut(s) 726
Csp6I GTAC 5 cut(s) 4, 373, 501, 581, 850
CviAII CATG 2 cut(s) 217, 719
CviQI GTAC 5 cut(s) 4, 373, 501, 581, 850
DdeI CTNAG 3 cut(s) 50, 369, 864
DpnI GATC 3 cut(s) 237, 462, 717
DpnII GATC 3 cut(s) 235, 460, 715
DraI TTTAAA 1 cut(s) 96
DriI GACNNNNNGTC 1 cut(s) 873
Eam1104I CTCTTC 2 cut(s) 134, 613
Eam1105I GACNNNNNGTC 1 cut(s) 873
EarI CTCTTC 2 cut(s) 134, 613
EciI GGCGGA 1 cut(s) 492
Eco24I GRGCYC 1 cut(s) 425
Eco31I GGTCTC 1 cut(s) 610
Eco91I GGTNACC 1 cut(s) 722
EcoNI CCTNNNNNAGG 1 cut(s) 294
EcoO65I GGTNACC 1 cut(s) 722
EcoRI GAATTC 1 cut(s) 903
EcoRII CCWGG 2 cut(s) 295, 726
EcoT38I GRGCYC 1 cut(s) 425
FaeI CATG 2 cut(s) 220, 722
FalI AAGNNNNNCTT 2 cut(s) 33, 65
FaqI GGGAC 2 cut(s) 679, 927
FatI CATG 2 cut(s) 216, 718
FbaI TGATCA 2 cut(s) 235, 715
Fnu4HI GCNGC 3 cut(s) 84, 87, 820
FokI GGATG 3 cut(s) 202, 572, 856
FriOI GRGCYC 1 cut(s) 425
Fsp4HI GCNGC 3 cut(s) 84, 87, 820
FspBI CTAG 3 cut(s) 170, 534, 896
GlaI GCGC 1 cut(s) 202
GluI GCNGC 3 cut(s) 84, 87, 820
GsuI CTGGAG 3 cut(s) 279, 504, 617
HaeIII GGCC 3 cut(s) 45, 342, 837
HapII CCGG 3 cut(s) 398, 515, 749
HhaI GCGC 1 cut(s) 203
Hin1II CATG 2 cut(s) 220, 722
Hin6I GCGC 1 cut(s) 201
HinP1I GCGC 1 cut(s) 201
HinfI GANTC 2 cut(s) 184, 575
HpaII CCGG 3 cut(s) 398, 515, 749
HphI GGTGA 1 cut(s) 357
Hpy166II GTNNAC 4 cut(s) 375, 583, 754, 878
Hpy188I TCNGA 3 cut(s) 189, 404, 559
Hpy188III TCNNGA 5 cut(s) 170, 572, 596, 678, 713
Hpy8I GTNNAC 4 cut(s) 375, 583, 754, 878
Hpy99I CGWCG 2 cut(s) 29, 664
HpyAV CCTTC 3 cut(s) 113, 231, 517
HpyCH4III ACNGT 2 cut(s) 228, 871
HpyCH4IV ACGT 1 cut(s) 659
HpyCH4V TGCA 5 cut(s) 86, 216, 428, 615, 786
HpyF3I CTNAG 3 cut(s) 50, 369, 864
HpySE526I ACGT 1 cut(s) 659
Hsp92II CATG 2 cut(s) 220, 722
HspAI GCGC 1 cut(s) 201
Ksp22I TGATCA 2 cut(s) 235, 715
Kzo9I GATC 3 cut(s) 235, 460, 715
LmnI GCTCC 1 cut(s) 479
Lsp1109I GCAGC 3 cut(s) 70, 98, 831
LweI GCATC 1 cut(s) 238
MabI ACCWGGT 1 cut(s) 726
MaeI CTAG 3 cut(s) 170, 534, 896
MaeII ACGT 1 cut(s) 659
MaeIII GTNAC 3 cut(s) 682, 722, 827
MalI GATC 3 cut(s) 237, 462, 717
MboI GATC 3 cut(s) 235, 460, 715
MboII GAAGA 4 cut(s) 9, 151, 205, 600
MhlI GDGCHC 1 cut(s) 425
MluCI AATT 9 cut(s) 32, 97, 239, 406, 505, 642, 758, 903, 948
MluI ACGCGT 1 cut(s) 739
MmeI TCCRAC 2 cut(s) 45, 382
MseI TTAA 2 cut(s) 75, 95
MspI CCGG 3 cut(s) 398, 515, 749
MspR9I CCNGG 4 cut(s) 297, 398, 515, 728
MvaI CCWGG 2 cut(s) 297, 728
MvnI CGCG 3 cut(s) 182, 203, 741
NciI CCSGG 2 cut(s) 398, 515
NdeII GATC 3 cut(s) 235, 460, 715
NlaIII CATG 2 cut(s) 220, 722
NlaIV GGNNCC 3 cut(s) 288, 343, 360
NspI RCATGY 1 cut(s) 220
PaeI GCATGC 1 cut(s) 220
PcsI WCGNNNNNNNCGW 2 cut(s) 469, 738
PfeI GAWTC 2 cut(s) 184, 575
PflMI CCANNNNNTGG 1 cut(s) 844
PkrI GCNGC 3 cut(s) 85, 88, 821
Psp6I CCWGG 2 cut(s) 295, 726
PspEI GGTNACC 1 cut(s) 722
PspGI CCWGG 2 cut(s) 295, 726
PspN4I GGNNCC 3 cut(s) 288, 343, 360
PspPI GGNCC 1 cut(s) 341
PstI CTGCAG 1 cut(s) 88
RsaI GTAC 5 cut(s) 5, 374, 502, 582, 851
RsaNI GTAC 5 cut(s) 4, 373, 501, 581, 850
SaqAI TTAA 2 cut(s) 75, 95
SatI GCNGC 3 cut(s) 84, 87, 820
Sau3AI GATC 3 cut(s) 235, 460, 715
Sau96I GGNCC 1 cut(s) 341
ScrFI CCNGG 4 cut(s) 297, 398, 515, 728
SduI GDGCHC 1 cut(s) 425
SetI ASST 8 cut(s) 268, 370, 484, 490, 606, 662, 712, 732
SexAI ACCWGGT 1 cut(s) 726
SfaNI GCATC 1 cut(s) 238
SfcI CTRYAG 1 cut(s) 84
SmlI CTYRAG 1 cut(s) 267
SmoI CTYRAG 1 cut(s) 267
SphI GCATGC 1 cut(s) 220
Sse9I AATT 9 cut(s) 32, 97, 239, 406, 505, 642, 758, 903, 948
SsiI CCGC 2 cut(s) 203, 477
SspMI CTAG 3 cut(s) 170, 534, 896
StyD4I CCNGG 4 cut(s) 295, 396, 513, 726
TaaI ACNGT 2 cut(s) 228, 871
TaiI ACGT 1 cut(s) 662
TaqI TCGA 4 cut(s) 69, 447, 688, 732
TasI AATT 9 cut(s) 32, 97, 239, 406, 505, 642, 758, 903, 948
TatI WGTACW 3 cut(s) 3, 372, 849
TfiI GAWTC 2 cut(s) 184, 575
Tru1I TTAA 2 cut(s) 75, 95
Tru9I TTAA 2 cut(s) 75, 95
TscAI CASTG 2 cut(s) 231, 793
TseI GCWGC 3 cut(s) 83, 86, 819
TspDTI ATGAA 6 cut(s) 26, 132, 206, 232, 613, 789
TspRI CASTG 2 cut(s) 231, 793
Van91I CCANNNNNTGG 1 cut(s) 844
XagI CCTNNNNNAGG 1 cut(s) 294
XapI RAATTY 2 cut(s) 32, 903
XbaI TCTAGA 1 cut(s) 169
XceI RCATGY 1 cut(s) 220
XcmI CCANNNNNNNNNTGG 1 cut(s) 352
XspI CTAG 3 cut(s) 170, 534, 896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.