pycom08g07050
ERF Family

Interferon-related developmental regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008512
Physical Location & Seq
Forward (+)
5641071 .. 5643983
2913 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g07050.9

Sequence Viewer

Length: 1335 bp
ATGGGGAAACGTAGTTCTCAGCGCAAGAATGCTGCAATGATGGATAGTGATGATGATAATAGTAGCGTGAGTTCAACGTCGACTACGCGCTCCGATCATGTTTCGGTGTCTGTCAATGAAGATATGCATGTGGATAAGGATAGTTTACTTGACCAGGCCGTAGATGCTTTGTATGAGAAGAGGGGTTCCACAAGAGAAAAGGCTTTGTCAGCAATTATTGACGCGTTTAATAGCAACATGCAACATCAGTTTGTGGAAAAGAAGTTTGCCACTTTATTGCATCAGTGTCTGAATTGCATCAAAAAGGGTTCGTCCAAAGAGATATGTTTGGCGTCTCATGTGATCGGACTATTGGCTTTGACTGTTGGATGTGGAGACAGTGCTCATGAAATACTGGAAGAATCACTACCTGTGATTTCACAGGCTTTCAAATCTGGATCTGAAGTCTCAAAGACATCAGCGTTACTGGAGTGTTTAGCTATTATCACTTTTGTTGGTGGTAGTGATCCAGAGCAAACAGAAAAGTCAATGCAAATTATGTGGCAAGTAGTTCATCCAAAACAAAGTTCCAATGTAATTGCAGTCAAACTCTCAGCAGCTGCAATAACCACCATGGTGTCTGCGTGGTCCTTTCTTTTGACAACTGTAGATGGGTGGAATCTTAATTTTAAAGATTGGCAAGAGTCCATAACATACTTATCTAGCCTGCTAGATAAGGATGACCGGTCTATGCGTATAGCCGCTGGTGAAGCTTTGGCATTAATTTTTGAGATTGGAAGTTTAGAAAAGTTCTCTGCTGGTGCTAATACTTCAAGTGATGCCTCAACTGAAGAAGGAAATAAACCCCGGGAATTTGTACATATACAAGGACTGAAATCAAAGATTATTAATCAAGCCAGAAACCTCTCAGCTGAGGCAGGTGGAAAAAGTTCTGCTAAAAAAGATCTTAGCAATCAGCGGAACATGTTCCGGGATATCTTGGAATATTTTGAGGATGGTTATTCTCCTGAAATTTCAATAAAGATTGGTGGTGAATCATTACACACATCAACGTGGTTGCAGATGATACAGTTGAACTTTTTGAAGCGTTTTCTTGGAGGAGGTTTTATCAAGCACATGCAGGAAAATGAACTCCTCCAGGATGTTCTTGGCTTTAATCCAAAGAAAAAGAATCTATCTGATTCCGAACATCGTCTATCTGGTGGTGAAAAGGTTCGTTCGTCACCGAACTCGGTTCAAAGCAAAGCAAGGACCCAGCTGCTTAACAAGCAGCGAATGTTATCCGAGGGTAAAAATATCGGCCGCTTTGCAGCGAACATGGGGGACGATGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IFRD IFRD_C

Protein Analysis

445

Amino Acids

48.77

Weight (kDa)

6.29

Isoelectric Point (pI)

40.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 908
Acc36I ACCTGC 1 cut(s) 908
AccI GTMKAC 1 cut(s) 80
AccII CGCG 2 cut(s) 88, 224
AciI CCGC 3 cut(s) 741, 958, 1303
AclWI GGATC 2 cut(s) 445, 500
AcoI YGGCCR 1 cut(s) 1300
AcsI RAATTY 2 cut(s) 851, 1011
AcuI CTGAAG 2 cut(s) 462, 849
AcyI GRCGYC 1 cut(s) 332
AfaI GTAC 1 cut(s) 858
AflIII ACRYGT 2 cut(s) 222, 963
AgeI ACCGGT 1 cut(s) 723
AgsI TTSAA 7 cut(s) 75, 430, 813, 1017, 1075, 1084, 1238
AjnI CCWGG 2 cut(s) 153, 1137
AjuI GAANNNNNNNTTGG 2 cut(s) 550, 582
AleI CACNNNNGTG 1 cut(s) 614
AloI GAACNNNNNNTCC 1 cut(s) 30
AluBI AGCT 5 cut(s) 479, 599, 752, 911, 1258
AluI AGCT 5 cut(s) 479, 599, 752, 911, 1258
Alw21I GWGCWC 1 cut(s) 385
Alw26I GTCTC 3 cut(s) 339, 369, 451
AlwI GGATC 2 cut(s) 445, 500
AlwNI CAGNNNCTG 2 cut(s) 289, 599
Ama87I CYCGRG 1 cut(s) 846
AoxI GGCC 2 cut(s) 156, 1300
ApeKI GCWGC 6 cut(s) 32, 596, 599, 1258, 1270, 1310
ApoI RAATTY 2 cut(s) 851, 1011
AseI ATTAAT 2 cut(s) 761, 888
AsiGI ACCGGT 1 cut(s) 723
Asp700I GAANNNNTTC 3 cut(s) 928, 965, 1212
AspLEI GCGC 2 cut(s) 24, 90
AspS9I GGNCC 2 cut(s) 627, 1251
AsuC2I CCSGG 3 cut(s) 847, 848, 971
AsuHPI GGTGA 4 cut(s) 758, 1043, 1215, 1217
AvaI CYCGRG 1 cut(s) 846
AvaII GGWCC 2 cut(s) 627, 1251
BarI GAAGNNNNNNTAC 2 cut(s) 390, 422
Bbv12I GWGCWC 1 cut(s) 385
BbvCI CCTCAGC 1 cut(s) 912
BbvI GCAGC 6 cut(s) 19, 586, 608, 1245, 1282, 1322
BccI CCATC 3 cut(s) 34, 644, 989
BceAI ACGGC 1 cut(s) 143
BciT130I CCWGG 2 cut(s) 155, 1139
BcnI CCSGG 3 cut(s) 847, 848, 971
BcoDI GTCTC 3 cut(s) 339, 369, 451
BfaI CTAG 2 cut(s) 702, 710
BfmI CTRYAG 1 cut(s) 645
BfuAI ACCTGC 1 cut(s) 908
BglII AGATCT 1 cut(s) 943
BisI GCNGC 8 cut(s) 33, 597, 600, 741, 1259, 1271, 1303, 1311
BlsI GCNGC 8 cut(s) 34, 598, 601, 742, 1260, 1272, 1304, 1312
Bme1390I CCNGG 5 cut(s) 155, 847, 848, 971, 1139
Bme18I GGWCC 2 cut(s) 627, 1251
BmeT110I CYCGRG 1 cut(s) 846
BmgT120I GGNCC 2 cut(s) 627, 1251
BmiI GGNNCC 2 cut(s) 187, 1253
BmrFI CCNGG 5 cut(s) 155, 847, 848, 971, 1139
BmsI GCATC 4 cut(s) 154, 289, 306, 808
BpmI CTGGAG 2 cut(s) 488, 1121
Bpu10I CCTNAGC 1 cut(s) 912
BpuMI CCSGG 3 cut(s) 847, 848, 971
BsaHI GRCGYC 1 cut(s) 332
BsaJI CCNNGG 4 cut(s) 612, 845, 846, 1284
BsaWI WCCGGW 1 cut(s) 723
Bse118I RCCGGY 1 cut(s) 723
Bse1I ACTGG 2 cut(s) 399, 471
Bse3DI GCAATG 1 cut(s) 42
BseBI CCWGG 2 cut(s) 155, 1139
BseDI CCNNGG 4 cut(s) 612, 845, 846, 1284
BseGI GGATG 5 cut(s) 374, 553, 724, 1000, 1147
BseMI GCAATG 1 cut(s) 42
BseMII CTCAG 4 cut(s) 32, 606, 903, 921
BseNI ACTGG 2 cut(s) 399, 471
BseRI GAGGAG 2 cut(s) 1113, 1124
BseX3I CGGCCG 1 cut(s) 1300
BseXI GCAGC 6 cut(s) 19, 586, 608, 1245, 1282, 1322
BseYI CCCAGC 1 cut(s) 1254
Bsh1236I CGCG 2 cut(s) 88, 224
Bsh1285I CGRYCG 1 cut(s) 1303
BshFI GGCC 2 cut(s) 158, 1302
BshTI ACCGGT 1 cut(s) 723
BsiEI CGRYCG 1 cut(s) 1303
BsiHKAI GWGCWC 1 cut(s) 385
BsiHKCI CYCGRG 1 cut(s) 846
BsiSI CCGG 3 cut(s) 724, 847, 970
BsmAI GTCTC 3 cut(s) 339, 369, 451
BsmBI CGTCTC 1 cut(s) 339
BsmI GAATGC 1 cut(s) 34
BsnI GGCC 2 cut(s) 158, 1302
BsoBI CYCGRG 1 cut(s) 846
Bsp1286I GDGCHC 1 cut(s) 385
Bsp1407I TGTACA 1 cut(s) 856
Bsp143I GATC 5 cut(s) 94, 342, 437, 505, 943
Bsp19I CCATGG 1 cut(s) 612
BspACI CCGC 3 cut(s) 741, 958, 1303
BspANI GGCC 2 cut(s) 158, 1302
BspCNI CTCAG 4 cut(s) 31, 605, 904, 920
BspFNI CGCG 2 cut(s) 88, 224
BspHI TCATGA 1 cut(s) 385
BspLI GGNNCC 2 cut(s) 187, 1253
BspMI ACCTGC 1 cut(s) 908
BspPI GGATC 2 cut(s) 445, 500
BsrDI GCAATG 1 cut(s) 42
BsrFI RCCGGY 1 cut(s) 723
BsrGI TGTACA 1 cut(s) 856
BsrI ACTGG 2 cut(s) 399, 471
BssAI RCCGGY 1 cut(s) 723
BssECI CCNNGG 4 cut(s) 612, 845, 846, 1284
BssMI GATC 5 cut(s) 94, 342, 437, 505, 943
BssNI GRCGYC 1 cut(s) 332
BssT1I CCWWGG 1 cut(s) 612
Bst2UI CCWGG 2 cut(s) 155, 1139
Bst4CI ACNGT 4 cut(s) 364, 380, 646, 1071
Bst6I CTCTTC 1 cut(s) 173
BstACI GRCGYC 1 cut(s) 332
BstAUI TGTACA 1 cut(s) 856
BstC8I GCNNGC 1 cut(s) 707
BstDEI CTNAG 5 cut(s) 18, 592, 907, 912, 947
BstDSI CCRYGG 1 cut(s) 612
BstF5I GGATG 5 cut(s) 374, 553, 724, 1000, 1147
BstFNI CGCG 2 cut(s) 88, 224
BstHHI GCGC 2 cut(s) 24, 90
BstKTI GATC 5 cut(s) 97, 345, 440, 508, 946
BstMAI GTCTC 3 cut(s) 339, 369, 451
BstMBI GATC 5 cut(s) 94, 342, 437, 505, 943
BstMCI CGRYCG 1 cut(s) 1303
BstMWI GCNNNNNNNGC 4 cut(s) 164, 209, 749, 1267
BstNI CCWGG 2 cut(s) 155, 1139
BstNSI RCATGY 4 cut(s) 131, 241, 967, 1120
BstSCI CCNGG 5 cut(s) 153, 845, 846, 969, 1137
BstSFI CTRYAG 1 cut(s) 645
BstUI CGCG 2 cut(s) 88, 224
BstV1I GCAGC 6 cut(s) 19, 586, 608, 1245, 1282, 1322
BstX2I RGATCY 2 cut(s) 437, 943
BstYI RGATCY 2 cut(s) 437, 943
BstZI CGGCCG 1 cut(s) 1300
BsuRI GGCC 2 cut(s) 158, 1302
BtgI CCRYGG 1 cut(s) 612
BtsCI GGATG 5 cut(s) 374, 553, 724, 1000, 1147
BtsIMutI CAGTG 2 cut(s) 290, 385
BveI ACCTGC 1 cut(s) 908
Cac8I GCNNGC 1 cut(s) 707
CaiI CAGNNNCTG 2 cut(s) 289, 599
CciI TCATGA 1 cut(s) 385
CfoI GCGC 2 cut(s) 24, 90
Cfr10I RCCGGY 1 cut(s) 723
Cfr13I GGNCC 2 cut(s) 627, 1251
Cfr9I CCCGGG 1 cut(s) 846
CseI GACGC 2 cut(s) 230, 321
Csp6I GTAC 1 cut(s) 857
CspAI ACCGGT 1 cut(s) 723
CspCI CAANNNNNGTGG 2 cut(s) 521, 556
CviAII CATG 9 cut(s) 98, 128, 238, 338, 386, 613, 964, 1117, 1318
CviQI GTAC 1 cut(s) 857
DdeI CTNAG 5 cut(s) 18, 592, 907, 912, 947
DpnI GATC 5 cut(s) 96, 344, 439, 507, 945
DpnII GATC 5 cut(s) 94, 342, 437, 505, 943
DraI TTTAAA 1 cut(s) 670
EaeI YGGCCR 1 cut(s) 1300
EagI CGGCCG 1 cut(s) 1300
Eam1104I CTCTTC 1 cut(s) 173
EarI CTCTTC 1 cut(s) 173
EclXI CGGCCG 1 cut(s) 1300
Eco130I CCWWGG 1 cut(s) 612
Eco32I GATATC 1 cut(s) 976
Eco47I GGWCC 2 cut(s) 627, 1251
Eco52I CGGCCG 1 cut(s) 1300
Eco57I CTGAAG 2 cut(s) 462, 849
Eco88I CYCGRG 1 cut(s) 846
EcoO109I RGGNCCY 1 cut(s) 1251
EcoRII CCWGG 2 cut(s) 153, 1137
EcoRV GATATC 1 cut(s) 976
EcoT14I CCWWGG 1 cut(s) 612
EcoT22I ATGCAT 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 612
Esp3I CGTCTC 1 cut(s) 339
FaeI CATG 9 cut(s) 101, 131, 241, 341, 389, 616, 967, 1120, 1321
FatI CATG 9 cut(s) 97, 127, 237, 337, 385, 612, 963, 1116, 1317
FblI GTMKAC 1 cut(s) 80
Fnu4HI GCNGC 8 cut(s) 33, 597, 600, 741, 1259, 1271, 1303, 1311
FokI GGATG 5 cut(s) 381, 540, 731, 1007, 1154
Fsp4HI GCNGC 8 cut(s) 33, 597, 600, 741, 1259, 1271, 1303, 1311
FspBI CTAG 2 cut(s) 702, 710
GlaI GCGC 2 cut(s) 23, 89
GluI GCNGC 8 cut(s) 33, 597, 600, 741, 1259, 1271, 1303, 1311
GsaI CCCAGC 1 cut(s) 1258
GsuI CTGGAG 2 cut(s) 488, 1121
HaeIII GGCC 2 cut(s) 158, 1302
HapII CCGG 3 cut(s) 724, 847, 970
HgaI GACGC 2 cut(s) 230, 321
HhaI GCGC 2 cut(s) 24, 90
Hin1I GRCGYC 1 cut(s) 332
Hin1II CATG 9 cut(s) 101, 131, 241, 341, 389, 616, 967, 1120, 1321
Hin6I GCGC 2 cut(s) 22, 88
HinP1I GCGC 2 cut(s) 22, 88
HincII GTYRAC 1 cut(s) 81
HindII GTYRAC 1 cut(s) 81
HindIII AAGCTT 1 cut(s) 750
HinfI GANTC 6 cut(s) 401, 658, 683, 1034, 1171, 1181
HpaII CCGG 3 cut(s) 724, 847, 970
HphI GGTGA 4 cut(s) 758, 1043, 1215, 1217
Hpy166II GTNNAC 2 cut(s) 81, 146
Hpy188I TCNGA 7 cut(s) 94, 291, 347, 442, 1180, 1186, 1285
Hpy188III TCNNGA 4 cut(s) 386, 435, 509, 1007
Hpy8I GTNNAC 2 cut(s) 81, 146
Hpy99I CGWCG 1 cut(s) 82
HpyAV CCTTC 1 cut(s) 827
HpyCH4III ACNGT 4 cut(s) 364, 380, 646, 1071
HpyCH4IV ACGT 3 cut(s) 10, 77, 1052
HpyF10VI GCNNNNNNNGC 4 cut(s) 164, 209, 749, 1267
HpyF3I CTNAG 5 cut(s) 18, 592, 907, 912, 947
HpySE526I ACGT 3 cut(s) 10, 77, 1052
Hsp92I GRCGYC 1 cut(s) 332
Hsp92II CATG 9 cut(s) 101, 131, 241, 341, 389, 616, 967, 1120, 1321
HspAI GCGC 2 cut(s) 22, 88
Kzo9I GATC 5 cut(s) 94, 342, 437, 505, 943
LmnI GCTCC 1 cut(s) 95
Lsp1109I GCAGC 6 cut(s) 19, 586, 608, 1245, 1282, 1322
LweI GCATC 4 cut(s) 154, 289, 306, 808
MaeI CTAG 2 cut(s) 702, 710
MaeII ACGT 3 cut(s) 10, 77, 1052
MaeIII GTNAC 2 cut(s) 462, 1221
MalI GATC 5 cut(s) 96, 344, 439, 507, 945
MboI GATC 5 cut(s) 94, 342, 437, 505, 943
MboII GAAGA 4 cut(s) 131, 190, 410, 842
MflI RGATCY 2 cut(s) 437, 943
MhlI GDGCHC 1 cut(s) 385
MluCI AATT 8 cut(s) 213, 292, 534, 576, 664, 762, 851, 1011
MluI ACGCGT 1 cut(s) 222
MlyI GAGTC 1 cut(s) 692
MmeI TCCRAC 1 cut(s) 346
MnlI CCTC 9 cut(s) 174, 832, 907, 914, 985, 1091, 1094, 1145, 1279
Mph1103I ATGCAT 1 cut(s) 129
MroXI GAANNNNTTC 3 cut(s) 928, 965, 1212
MseI TTAA 7 cut(s) 228, 663, 669, 761, 888, 1155, 1263
MslI CAYNNNNRTG 2 cut(s) 614, 1051
MspA1I CMGCKG 5 cut(s) 599, 743, 911, 958, 1258
MspI CCGG 3 cut(s) 724, 847, 970
MspR9I CCNGG 5 cut(s) 155, 847, 848, 971, 1139
Mva1269I GAATGC 1 cut(s) 34
MvaI CCWGG 2 cut(s) 155, 1139
MvnI CGCG 2 cut(s) 88, 224
MwoI GCNNNNNNNGC 4 cut(s) 164, 209, 749, 1267
NciI CCSGG 3 cut(s) 847, 848, 971
NcoI CCATGG 1 cut(s) 612
NdeII GATC 5 cut(s) 94, 342, 437, 505, 943
NlaIII CATG 9 cut(s) 101, 131, 241, 341, 389, 616, 967, 1120, 1321
NlaIV GGNNCC 2 cut(s) 187, 1253
NmuCI GTSAC 1 cut(s) 1221
NsiI ATGCAT 1 cut(s) 129
NspI RCATGY 4 cut(s) 131, 241, 967, 1120
OliI CACNNNNGTG 1 cut(s) 614
PagI TCATGA 1 cut(s) 385
PaqCI CACCTGC 1 cut(s) 908
PciI ACATGT 1 cut(s) 963
PctI GAATGC 1 cut(s) 34
PdmI GAANNNNTTC 3 cut(s) 928, 965, 1212
PfeI GAWTC 5 cut(s) 401, 658, 1034, 1171, 1181
PfoI TCCNGGA 2 cut(s) 969, 1137
PinAI ACCGGT 1 cut(s) 723
PkrI GCNGC 8 cut(s) 34, 598, 601, 742, 1260, 1272, 1304, 1312
PleI GAGTC 1 cut(s) 691
PpsI GAGTC 1 cut(s) 691
PpuMI RGGWCCY 1 cut(s) 1251
PscI ACATGT 1 cut(s) 963
PshBI ATTAAT 2 cut(s) 761, 888
Psp5II RGGWCCY 1 cut(s) 1251
Psp6I CCWGG 2 cut(s) 153, 1137
PspFI CCCAGC 1 cut(s) 1254
PspGI CCWGG 2 cut(s) 153, 1137
PspN4I GGNNCC 2 cut(s) 187, 1253
PspPI GGNCC 2 cut(s) 627, 1251
PspPPI RGGWCCY 1 cut(s) 1251
PsrI GAACNNNNNNTAC 2 cut(s) 55, 87
PstNI CAGNNNCTG 2 cut(s) 289, 599
PsuI RGATCY 2 cut(s) 437, 943
PvuII CAGCTG 3 cut(s) 599, 911, 1258
RsaI GTAC 1 cut(s) 858
RsaNI GTAC 1 cut(s) 857
RseI CAYNNNNRTG 2 cut(s) 614, 1051
SalI GTCGAC 1 cut(s) 79
SaqAI TTAA 7 cut(s) 228, 663, 669, 761, 888, 1155, 1263
SatI GCNGC 8 cut(s) 33, 597, 600, 741, 1259, 1271, 1303, 1311
Sau3AI GATC 5 cut(s) 94, 342, 437, 505, 943
Sau96I GGNCC 2 cut(s) 627, 1251
SchI GAGTC 1 cut(s) 692
ScrFI CCNGG 5 cut(s) 155, 847, 848, 971, 1139
SduI GDGCHC 1 cut(s) 385
SfaNI GCATC 4 cut(s) 154, 289, 306, 808
SfcI CTRYAG 1 cut(s) 645
SinI GGWCC 2 cut(s) 627, 1251
SmaI CCCGGG 1 cut(s) 848
SmiMI CAYNNNNRTG 2 cut(s) 614, 1051
Sse9I AATT 8 cut(s) 213, 292, 534, 576, 664, 762, 851, 1011
SsiI CCGC 3 cut(s) 741, 958, 1303
SspI AATATT 1 cut(s) 986
SspMI CTAG 2 cut(s) 702, 710
StyD4I CCNGG 5 cut(s) 153, 845, 846, 969, 1137
StyI CCWWGG 1 cut(s) 612
TaaI ACNGT 4 cut(s) 364, 380, 646, 1071
TaiI ACGT 3 cut(s) 13, 80, 1055
TaqI TCGA 1 cut(s) 80
TasI AATT 8 cut(s) 213, 292, 534, 576, 664, 762, 851, 1011
TatI WGTACW 1 cut(s) 856
TauI GCSGC 2 cut(s) 743, 1305
TfiI GAWTC 5 cut(s) 401, 658, 1034, 1171, 1181
Tru1I TTAA 7 cut(s) 228, 663, 669, 761, 888, 1155, 1263
Tru9I TTAA 7 cut(s) 228, 663, 669, 761, 888, 1155, 1263
TscAI CASTG 2 cut(s) 290, 385
TseFI GTSAC 1 cut(s) 1221
TseI GCWGC 6 cut(s) 32, 596, 599, 1258, 1270, 1310
Tsp45I GTSAC 1 cut(s) 1221
TspDTI ATGAA 4 cut(s) 132, 402, 542, 1143
TspMI CCCGGG 1 cut(s) 846
TspRI CASTG 2 cut(s) 290, 385
VpaK11BI GGWCC 2 cut(s) 627, 1251
VspI ATTAAT 2 cut(s) 761, 888
XapI RAATTY 2 cut(s) 851, 1011
XceI RCATGY 4 cut(s) 131, 241, 967, 1120
XcmI CCANNNNNNNNNTGG 1 cut(s) 1145
XmaI CCCGGG 1 cut(s) 846
XmiI GTMKAC 1 cut(s) 80
XmnI GAANNNNTTC 3 cut(s) 928, 965, 1212
XspI CTAG 2 cut(s) 702, 710
Zsp2I ATGCAT 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.