pycom08g07510

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0013625
Physical Location & Seq
Forward (+)
6050182 .. 6050634
453 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 333 bp
ATGATACGTGCCCGTCCCCGGTGCCCCCTTGTTTCCACACTATTGCTTGACCGCGACGAACTAAACTATATCTACCATCTACGCCTTGGGCAGCTGATCATGTTATGTTTGGAGGTGCAAGTTTCAGACTCAGTTCAGTTTCGCAACATGAATATTTCAAACGGAACTGAAGAAATAAACGAGCAAGGAGCAGTGGAAATCAAGGTGGACACAGTTGATTATCGGTCGCCGGCAGGGGAAGACAAACAGCCCAAGAAGGAGAAGGTTGAGGTGGTTCATGAGATTCGAGATGGTGGAAATAAGGGGGCTGCAGGAGGAGGTAGTGACCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

111

Amino Acids

12.3

Weight (kDa)

5.38

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 21
AccII CGCG 1 cut(s) 54
AciI CCGC 1 cut(s) 52
AcuI CTGAAG 1 cut(s) 189
AfiI CCNNNNNNNGG 1 cut(s) 18
AgsI TTSAA 1 cut(s) 159
AluBI AGCT 1 cut(s) 94
AluI AGCT 1 cut(s) 94
ApeKI GCWGC 2 cut(s) 91, 308
AsuC2I CCSGG 1 cut(s) 19
BaeGI GKGCMC 2 cut(s) 13, 26
BanI GGYRCC 1 cut(s) 21
BbsI GAAGAC 1 cut(s) 246
BbvI GCAGC 2 cut(s) 103, 295
BccI CCATC 2 cut(s) 84, 284
BclI TGATCA 1 cut(s) 96
BcnI CCSGG 1 cut(s) 19
BfmI CTRYAG 1 cut(s) 309
BisI GCNGC 2 cut(s) 92, 309
BlsI GCNGC 2 cut(s) 93, 310
Bme1390I CCNGG 1 cut(s) 19
BmiI GGNNCC 1 cut(s) 23
BmrFI CCNGG 1 cut(s) 19
BpiI GAAGAC 1 cut(s) 246
BpuMI CCSGG 1 cut(s) 19
BsaAI YACGTR 1 cut(s) 8
BsaJI CCNNGG 2 cut(s) 17, 85
Bsc4I CCNNNNNNNGG 1 cut(s) 18
Bse118I RCCGGY 1 cut(s) 229
BseDI CCNNGG 2 cut(s) 17, 85
BseLI CCNNNNNNNGG 1 cut(s) 18
BseMII CTCAG 1 cut(s) 144
BseRI GAGGAG 1 cut(s) 330
BseSI GKGCMC 2 cut(s) 13, 26
BseXI GCAGC 2 cut(s) 103, 295
Bsh1236I CGCG 1 cut(s) 54
Bsh1285I CGRYCG 1 cut(s) 227
BshNI GGYRCC 1 cut(s) 21
BsiEI CGRYCG 1 cut(s) 227
BsiSI CCGG 2 cut(s) 19, 230
BslI CCNNNNNNNGG 1 cut(s) 18
Bsp1286I GDGCHC 2 cut(s) 13, 26
Bsp143I GATC 1 cut(s) 96
BspACI CCGC 1 cut(s) 52
BspCNI CTCAG 1 cut(s) 143
BspFNI CGCG 1 cut(s) 54
BspHI TCATGA 1 cut(s) 277
BspLI GGNNCC 1 cut(s) 23
BspMAI CTGCAG 1 cut(s) 313
BspT107I GGYRCC 1 cut(s) 21
BsrFI RCCGGY 1 cut(s) 229
BssAI RCCGGY 1 cut(s) 229
BssECI CCNNGG 2 cut(s) 17, 85
BssMI GATC 1 cut(s) 96
BssT1I CCWWGG 1 cut(s) 85
Bst4CI ACNGT 2 cut(s) 214, 329
BstBAI YACGTR 1 cut(s) 8
BstC8I GCNNGC 1 cut(s) 231
BstDEI CTNAG 1 cut(s) 130
BstFNI CGCG 1 cut(s) 54
BstKTI GATC 1 cut(s) 99
BstMBI GATC 1 cut(s) 96
BstMCI CGRYCG 1 cut(s) 227
BstSCI CCNGG 1 cut(s) 17
BstSFI CTRYAG 1 cut(s) 309
BstSLI GKGCMC 2 cut(s) 13, 26
BstUI CGCG 1 cut(s) 54
BstV1I GCAGC 2 cut(s) 103, 295
BstV2I GAAGAC 1 cut(s) 246
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 1 cut(s) 198
Cac8I GCNNGC 1 cut(s) 231
CciI TCATGA 1 cut(s) 277
Cfr10I RCCGGY 1 cut(s) 229
CviAII CATG 3 cut(s) 100, 148, 278
CviJI RGCY 3 cut(s) 94, 250, 308
CviKI_1 RGCY 3 cut(s) 94, 250, 308
DdeI CTNAG 1 cut(s) 130
DpnI GATC 1 cut(s) 98
DpnII GATC 1 cut(s) 96
Eco130I CCWWGG 1 cut(s) 85
Eco57I CTGAAG 1 cut(s) 189
EcoT14I CCWWGG 1 cut(s) 85
ErhI CCWWGG 1 cut(s) 85
FaeI CATG 3 cut(s) 103, 151, 281
FaiI YATR 5 cut(s) 69, 101, 106, 149, 279
FatI CATG 3 cut(s) 99, 147, 277
FbaI TGATCA 1 cut(s) 96
Fnu4HI GCNGC 2 cut(s) 92, 309
Fsp4HI GCNGC 2 cut(s) 92, 309
GluI GCNGC 2 cut(s) 92, 309
HapII CCGG 2 cut(s) 19, 230
Hin1II CATG 3 cut(s) 103, 151, 281
HinfI GANTC 2 cut(s) 128, 283
HpaII CCGG 2 cut(s) 19, 230
Hpy166II GTNNAC 1 cut(s) 208
Hpy188I TCNGA 1 cut(s) 127
Hpy188III TCNNGA 2 cut(s) 278, 287
Hpy8I GTNNAC 1 cut(s) 208
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 2 cut(s) 250, 256
HpyCH4III ACNGT 2 cut(s) 214, 329
HpyCH4IV ACGT 1 cut(s) 7
HpyCH4V TGCA 2 cut(s) 118, 311
HpyF3I CTNAG 1 cut(s) 130
HpySE526I ACGT 1 cut(s) 7
Hsp92II CATG 3 cut(s) 103, 151, 281
KroI GCCGGC 1 cut(s) 229
KroNI GCCGGC 1 cut(s) 231
Ksp22I TGATCA 1 cut(s) 96
Kzo9I GATC 1 cut(s) 96
LmnI GCTCC 1 cut(s) 188
LpnPI CCDG 4 cut(s) 32, 219, 243, 297
Lsp1109I GCAGC 2 cut(s) 103, 295
MaeII ACGT 1 cut(s) 7
MaeIII GTNAC 1 cut(s) 323
MalI GATC 1 cut(s) 98
MboI GATC 1 cut(s) 96
MboII GAAGA 2 cut(s) 182, 251
MhlI GDGCHC 2 cut(s) 13, 26
MlyI GAGTC 1 cut(s) 122
MnlI CCTC 4 cut(s) 106, 262, 308, 311
MroNI GCCGGC 1 cut(s) 229
MspA1I CMGCKG 1 cut(s) 94
MspI CCGG 2 cut(s) 19, 230
MspR9I CCNGG 1 cut(s) 19
MvnI CGCG 1 cut(s) 54
NaeI GCCGGC 1 cut(s) 231
NciI CCSGG 1 cut(s) 19
NdeII GATC 1 cut(s) 96
NgoMIV GCCGGC 1 cut(s) 229
NlaIII CATG 3 cut(s) 103, 151, 281
NlaIV GGNNCC 1 cut(s) 23
NmuCI GTSAC 1 cut(s) 323
PagI TCATGA 1 cut(s) 277
PdiI GCCGGC 1 cut(s) 231
PfeI GAWTC 1 cut(s) 283
PkrI GCNGC 2 cut(s) 93, 310
PleI GAGTC 1 cut(s) 122
PpsI GAGTC 1 cut(s) 122
Ppu21I YACGTR 1 cut(s) 8
PspN4I GGNNCC 1 cut(s) 23
PstI CTGCAG 1 cut(s) 313
PvuII CAGCTG 1 cut(s) 94
SatI GCNGC 2 cut(s) 92, 309
Sau3AI GATC 1 cut(s) 96
SchI GAGTC 1 cut(s) 122
ScrFI CCNGG 1 cut(s) 19
SduI GDGCHC 2 cut(s) 13, 26
SetI ASST 7 cut(s) 10, 96, 117, 207, 267, 273, 322
SfcI CTRYAG 1 cut(s) 309
SsiI CCGC 1 cut(s) 52
SspI AATATT 1 cut(s) 154
StyD4I CCNGG 1 cut(s) 17
StyI CCWWGG 1 cut(s) 85
TaaI ACNGT 2 cut(s) 214, 329
TaiI ACGT 1 cut(s) 10
TaqI TCGA 1 cut(s) 286
TaqII GACCGA 1 cut(s) 213
TfiI GAWTC 1 cut(s) 283
TscAI CASTG 1 cut(s) 198
TseFI GTSAC 1 cut(s) 323
TseI GCWGC 2 cut(s) 91, 308
Tsp45I GTSAC 1 cut(s) 323
TspDTI ATGAA 2 cut(s) 164, 266
TspGWI ACGGA 1 cut(s) 177
TspRI CASTG 1 cut(s) 198
XcmI CCANNNNNNNNNTGG 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.