pycom08g08420

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0009593
Physical Location & Seq
Reverse (-)
6752340 .. 6756214
3875 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1293 bp
ATGTCCACCAGGAGCGAAAATCATCAGACGGTTCCGCTTTCGGTGCTGCTCAAGCGTGAATTGGCCAATGAGAAGATTGAGAGACCGGAGCTTATACACGGTCAGGCGAGTCAGAGCAAGAAAGGAGAGGATTTCACGTTGGTAAAGACGGAATGTCAAAGAATTGTGGGAGATGGAGTTTCTACGTATTCAGTTTTCGGGCTATTTGATGGGCATAACGGGTCAGCAGCCGCTATCTACTCTAAGGAGAATCTTCTGAATAATGTTTTGGCCGCTATCCCTTCAGATCTAAACAAAGATGAATGGGTAGCAGCATTGCCAAGGGCTTTTGTAGCAGGCTTCGTCAAGACAGACAAAGAATTCCAAGAGAGAGCTAAAACATCAGGAACAACTGTCACATTTGTGATTATAGAAGGATGGGTCATATCTGTTGCATCTGTTGGCGATTCTCGTTGCATAGTTGAACCTGCTGAAGGTGGAGTTTATTATTTATCAGCAGATCATAGGCTTGAAATCAATGAAGAGGAGAGGCAGCGTATCACCTCAAGTGGGGGTGAGGTTGGTCGGCTTAATACTGGTGGTGGAGCAGAGATCGGTCCTTTGAGGTGTTGGCCTGGAGGCTTGTGTCTGTCACGATCCATTGGTGATCTGGATGTGGGGGAGTTCATTGTTCCTGTTCCTTATGTGAAGCAAATCAAGTTGTCGAGTGCAGGTGGTAGAATTATCATGTCAAGTGACGGAGTCTGGGATGCTTTGTCTGCAGAAGAAGCTCTTGATTGTTGCCGTGGGATGCCACCGGACGCGGCGGCTGCACAAATTGTTAAGGATGCTGTAGGGCATAAGGGACTTCGGGATGATACAACCTGCATTGTGATTGATATTTTACCACAAGAGAAGCCACAAGCTCCGTTATCCCAACCAAAGAGGCATGGAAAAGGAGTGTTGTTTAAATCCATGTTTAAGAAAAAGTCCTCTGAATCATCTTCTAACGTTGAAAAAGAATATATTGAGCCAGACGAGGTGGAGGAATTATTTGAGGAAGGTTCTGCTTCACTTTCCGAAAGGTTAGATACAAAATACCCGCTCTGCAACATGTTCAAGTTGTTTATGTGTGCAGTTTGTCAATTAGAAATTGAACCGGGAGAGGGTATTTCAATACACGCTGGGTCATCGAACCCAGGAAAAGTGAGACCTTGGGATGGCCCCTTTCTTTGCTCGAGTTGCCAAGAGAAAAAAGAGGCCATGGAGGGGCAAAGACCATCAGGAAGAAGACGCGATAGCGATAGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

431

Amino Acids

46.77

Weight (kDa)

5.19

Isoelectric Point (pI)

49.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 58 - 274 3.3e-30 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 701
Acc36I ACCTGC 3 cut(s) 475, 701, 872
AccBSI CCGCTC 1 cut(s) 1084
AccII CGCG 2 cut(s) 803, 1275
AciI CCGC 6 cut(s) 35, 231, 273, 803, 806, 1082
AclI AACGTT 1 cut(s) 990
AclWI GGATC 1 cut(s) 630
AcoI YGGCCR 2 cut(s) 63, 270
AcsI RAATTY 1 cut(s) 359
AcuI CTGAAG 2 cut(s) 267, 492
AfiI CCNNNNNNNGG 5 cut(s) 473, 549, 1145, 1199, 1248
AflIII ACRYGT 1 cut(s) 1092
AgsI TTSAA 6 cut(s) 464, 512, 995, 1099, 1136, 1155
AhdI GACNNNNNGTC 1 cut(s) 153
AjnI CCWGG 3 cut(s) 8, 613, 1177
AjuI GAANNNNNNNTTGG 2 cut(s) 251, 283
AleI CACNNNNGTG 1 cut(s) 401
AluBI AGCT 4 cut(s) 91, 374, 770, 905
AluI AGCT 4 cut(s) 91, 374, 770, 905
Alw26I GTCTC 2 cut(s) 76, 1183
AlwI GGATC 1 cut(s) 630
Ama87I CYCGRG 1 cut(s) 1216
AoxI GGCC 5 cut(s) 63, 270, 611, 1201, 1239
ApeKI GCWGC 5 cut(s) 46, 227, 311, 532, 809
ApoI RAATTY 1 cut(s) 359
AspS9I GGNCC 2 cut(s) 596, 1202
AsuC2I CCSGG 1 cut(s) 1140
AsuHPI GGTGA 3 cut(s) 532, 566, 656
AvaI CYCGRG 1 cut(s) 1216
AvaII GGWCC 1 cut(s) 596
BalI TGGCCA 1 cut(s) 65
BbsI GAAGAC 1 cut(s) 1276
BbvI GCAGC 5 cut(s) 33, 239, 323, 544, 796
BccI CCATC 5 cut(s) 167, 203, 411, 1193, 1267
BceAI ACGGC 1 cut(s) 768
BciT130I CCWGG 3 cut(s) 10, 615, 1179
BcnI CCSGG 1 cut(s) 1140
BcoDI GTCTC 2 cut(s) 76, 1183
BfmI CTRYAG 2 cut(s) 759, 831
BfuAI ACCTGC 3 cut(s) 475, 701, 872
BglII AGATCT 1 cut(s) 286
BisI GCNGC 9 cut(s) 47, 228, 231, 273, 312, 533, 804, 807, 810
BlsI GCNGC 9 cut(s) 48, 229, 232, 274, 313, 534, 805, 808, 811
Bme1390I CCNGG 4 cut(s) 10, 615, 1140, 1179
Bme18I GGWCC 1 cut(s) 596
BmeRI GACNNNNNGTC 1 cut(s) 153
BmeT110I CYCGRG 1 cut(s) 1216
BmgT120I GGNCC 2 cut(s) 596, 1202
BmiI GGNNCC 2 cut(s) 33, 1204
BmrFI CCNGG 4 cut(s) 10, 615, 1140, 1179
BmsI GCATC 4 cut(s) 443, 739, 780, 817
BpiI GAAGAC 1 cut(s) 1276
BpmI CTGGAG 1 cut(s) 636
BpuEI CTTGAG 2 cut(s) 35, 529
BpuMI CCSGG 1 cut(s) 1140
BsaAI YACGTR 1 cut(s) 186
BsaI GGTCTC 2 cut(s) 76, 1183
BsaJI CCNNGG 5 cut(s) 320, 784, 1177, 1193, 1242
BsaWI WCCGGW 2 cut(s) 85, 796
BsaXI ACNNNNNCTCC 2 cut(s) 162, 192
Bsc4I CCNNNNNNNGG 5 cut(s) 473, 549, 1145, 1199, 1248
Bse1I ACTGG 1 cut(s) 580
Bse3DI GCAATG 1 cut(s) 314
BseBI CCWGG 3 cut(s) 10, 615, 1179
BseDI CCNNGG 5 cut(s) 320, 784, 1177, 1193, 1242
BseGI GGATG 7 cut(s) 422, 658, 754, 795, 832, 859, 1204
BseLI CCNNNNNNNGG 5 cut(s) 473, 549, 1145, 1199, 1248
BseMI GCAATG 1 cut(s) 314
BseNI ACTGG 1 cut(s) 580
BseRI GAGGAG 1 cut(s) 539
BseXI GCAGC 5 cut(s) 33, 239, 323, 544, 796
BseYI CCCAGC 1 cut(s) 1163
BsgI GTGCAG 3 cut(s) 729, 795, 1134
Bsh1236I CGCG 2 cut(s) 803, 1275
BshFI GGCC 5 cut(s) 65, 272, 613, 1203, 1241
BsiHKCI CYCGRG 1 cut(s) 1216
BsiSI CCGG 3 cut(s) 86, 797, 1139
BslFI GGGAC 1 cut(s) 858
BslI CCNNNNNNNGG 5 cut(s) 473, 549, 1145, 1199, 1248
BsmAI GTCTC 2 cut(s) 76, 1183
BsmFI GGGAC 1 cut(s) 858
BsnI GGCC 5 cut(s) 65, 272, 613, 1203, 1241
Bso31I GGTCTC 2 cut(s) 76, 1183
BsoBI CYCGRG 1 cut(s) 1216
Bsp143I GATC 5 cut(s) 286, 499, 591, 635, 646
Bsp19I CCATGG 1 cut(s) 1242
BspACI CCGC 6 cut(s) 35, 231, 273, 803, 806, 1082
BspANI GGCC 5 cut(s) 65, 272, 613, 1203, 1241
BspFNI CGCG 2 cut(s) 803, 1275
BspLI GGNNCC 2 cut(s) 33, 1204
BspMAI CTGCAG 1 cut(s) 763
BspMI ACCTGC 3 cut(s) 475, 701, 872
BspPI GGATC 1 cut(s) 630
BspTNI GGTCTC 2 cut(s) 76, 1183
BsrBI CCGCTC 1 cut(s) 1084
BsrDI GCAATG 1 cut(s) 314
BsrI ACTGG 1 cut(s) 580
BssECI CCNNGG 5 cut(s) 320, 784, 1177, 1193, 1242
BssMI GATC 5 cut(s) 286, 499, 591, 635, 646
BssT1I CCWWGG 3 cut(s) 320, 1193, 1242
Bst2UI CCWGG 3 cut(s) 10, 615, 1179
Bst4CI ACNGT 3 cut(s) 31, 101, 394
Bst6I CTCTTC 1 cut(s) 516
BstBAI YACGTR 1 cut(s) 186
BstC8I GCNNGC 1 cut(s) 337
BstDEI CTNAG 1 cut(s) 243
BstDSI CCRYGG 2 cut(s) 784, 1242
BstENI CCTNNNNNAGG 1 cut(s) 471
BstF5I GGATG 7 cut(s) 422, 658, 754, 795, 832, 859, 1204
BstFNI CGCG 2 cut(s) 803, 1275
BstKTI GATC 5 cut(s) 289, 502, 594, 638, 649
BstMAI GTCTC 2 cut(s) 76, 1183
BstMBI GATC 5 cut(s) 286, 499, 591, 635, 646
BstMWI GCNNNNNNNGC 7 cut(s) 43, 52, 332, 758, 767, 809, 1221
BstNI CCWGG 3 cut(s) 10, 615, 1179
BstNSI RCATGY 1 cut(s) 1096
BstSCI CCNGG 4 cut(s) 8, 613, 1138, 1177
BstSFI CTRYAG 2 cut(s) 759, 831
BstSNI TACGTA 1 cut(s) 186
BstUI CGCG 2 cut(s) 803, 1275
BstV1I GCAGC 5 cut(s) 33, 239, 323, 544, 796
BstV2I GAAGAC 1 cut(s) 1276
BstX2I RGATCY 1 cut(s) 286
BstYI RGATCY 1 cut(s) 286
BsuRI GGCC 5 cut(s) 65, 272, 613, 1203, 1241
BtgI CCRYGG 2 cut(s) 784, 1242
BtsCI GGATG 7 cut(s) 422, 658, 754, 795, 832, 859, 1204
BveI ACCTGC 3 cut(s) 475, 701, 872
Cac8I GCNNGC 1 cut(s) 337
Cfr13I GGNCC 2 cut(s) 596, 1202
CseI GACGC 2 cut(s) 809, 1281
CviAII CATG 5 cut(s) 727, 929, 955, 1093, 1243
DdeI CTNAG 1 cut(s) 243
DpnI GATC 5 cut(s) 288, 501, 593, 637, 648
DpnII GATC 5 cut(s) 286, 499, 591, 635, 646
DraI TTTAAA 1 cut(s) 949
DriI GACNNNNNGTC 1 cut(s) 153
EaeI YGGCCR 2 cut(s) 63, 270
Eam1104I CTCTTC 1 cut(s) 516
Eam1105I GACNNNNNGTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 516
Eco105I TACGTA 1 cut(s) 186
Eco130I CCWWGG 3 cut(s) 320, 1193, 1242
Eco31I GGTCTC 2 cut(s) 76, 1183
Eco47I GGWCC 1 cut(s) 596
Eco57I CTGAAG 2 cut(s) 267, 492
Eco88I CYCGRG 1 cut(s) 1216
EcoNI CCTNNNNNAGG 1 cut(s) 471
EcoRI GAATTC 1 cut(s) 359
EcoRII CCWGG 3 cut(s) 8, 613, 1177
EcoT14I CCWWGG 3 cut(s) 320, 1193, 1242
ErhI CCWWGG 3 cut(s) 320, 1193, 1242
FaeI CATG 5 cut(s) 730, 932, 958, 1096, 1246
FalI AAGNNNNNCTT 2 cut(s) 756, 788
FaqI GGGAC 1 cut(s) 858
FatI CATG 5 cut(s) 726, 928, 954, 1092, 1242
FauI CCCGC 1 cut(s) 1089
Fnu4HI GCNGC 9 cut(s) 47, 228, 231, 273, 312, 533, 804, 807, 810
FokI GGATG 7 cut(s) 429, 665, 761, 802, 839, 866, 1211
Fsp4HI GCNGC 9 cut(s) 47, 228, 231, 273, 312, 533, 804, 807, 810
GluI GCNGC 9 cut(s) 47, 228, 231, 273, 312, 533, 804, 807, 810
GsaI CCCAGC 1 cut(s) 1167
GsuI CTGGAG 1 cut(s) 636
HaeIII GGCC 5 cut(s) 65, 272, 613, 1203, 1241
HapII CCGG 3 cut(s) 86, 797, 1139
HgaI GACGC 2 cut(s) 809, 1281
Hin1II CATG 5 cut(s) 730, 932, 958, 1096, 1246
HinfI GANTC 5 cut(s) 109, 250, 446, 741, 977
HpaII CCGG 3 cut(s) 86, 797, 1139
HphI GGTGA 3 cut(s) 532, 566, 656
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 6 cut(s) 27, 114, 258, 286, 976, 1060
Hpy188III TCNNGA 7 cut(s) 346, 384, 633, 650, 773, 851, 1263
Hpy8I GTNNAC 1 cut(s) 6
HpyAV CCTTC 4 cut(s) 291, 407, 467, 1034
HpyCH4III ACNGT 3 cut(s) 31, 101, 394
HpyCH4IV ACGT 3 cut(s) 137, 185, 990
HpyCH4V TGCA 8 cut(s) 434, 456, 710, 761, 812, 867, 1089, 1115
HpyF10VI GCNNNNNNNGC 7 cut(s) 43, 52, 332, 758, 767, 809, 1221
HpyF3I CTNAG 1 cut(s) 243
HpySE526I ACGT 3 cut(s) 137, 185, 990
Hsp92II CATG 5 cut(s) 730, 932, 958, 1096, 1246
Kzo9I GATC 5 cut(s) 286, 499, 591, 635, 646
LmnI GCTCC 4 cut(s) 12, 88, 584, 910
Lsp1109I GCAGC 5 cut(s) 33, 239, 323, 544, 796
LweI GCATC 4 cut(s) 443, 739, 780, 817
MaeII ACGT 3 cut(s) 137, 185, 990
MaeIII GTNAC 3 cut(s) 394, 630, 734
MalI GATC 5 cut(s) 288, 501, 593, 637, 648
MbiI CCGCTC 1 cut(s) 1084
MboI GATC 5 cut(s) 286, 499, 591, 635, 646
MboII GAAGA 7 cut(s) 85, 245, 533, 776, 975, 1278, 1281
MflI RGATCY 1 cut(s) 286
MlsI TGGCCA 1 cut(s) 65
MluCI AATT 8 cut(s) 59, 162, 359, 720, 816, 1028, 1124, 1131
MluNI TGGCCA 1 cut(s) 65
MlyI GAGTC 2 cut(s) 118, 750
Mox20I TGGCCA 1 cut(s) 65
MscI TGGCCA 1 cut(s) 65
MseI TTAA 4 cut(s) 570, 822, 948, 960
MslI CAYNNNNRTG 1 cut(s) 401
Msp20I TGGCCA 1 cut(s) 65
MspI CCGG 3 cut(s) 86, 797, 1139
MspR9I CCNGG 4 cut(s) 10, 615, 1140, 1179
MvaI CCWGG 3 cut(s) 10, 615, 1179
MvnI CGCG 2 cut(s) 803, 1275
MwoI GCNNNNNNNGC 7 cut(s) 43, 52, 332, 758, 767, 809, 1221
NciI CCSGG 1 cut(s) 1140
NcoI CCATGG 1 cut(s) 1242
NdeII GATC 5 cut(s) 286, 499, 591, 635, 646
NlaIII CATG 5 cut(s) 730, 932, 958, 1096, 1246
NlaIV GGNNCC 2 cut(s) 33, 1204
NmuCI GTSAC 3 cut(s) 394, 630, 734
NspI RCATGY 1 cut(s) 1096
OliI CACNNNNGTG 1 cut(s) 401
PaeR7I CTCGAG 1 cut(s) 1216
PaqCI CACCTGC 1 cut(s) 701
PciI ACATGT 1 cut(s) 1092
PfeI GAWTC 3 cut(s) 250, 446, 977
PflFI GACNNNGTC 1 cut(s) 740
PkrI GCNGC 9 cut(s) 48, 229, 232, 274, 313, 534, 805, 808, 811
PleI GAGTC 2 cut(s) 117, 749
PpsI GAGTC 2 cut(s) 117, 749
Ppu21I YACGTR 1 cut(s) 186
PscI ACATGT 1 cut(s) 1092
Psp1406I AACGTT 1 cut(s) 990
Psp6I CCWGG 3 cut(s) 8, 613, 1177
PspFI CCCAGC 1 cut(s) 1163
PspGI CCWGG 3 cut(s) 8, 613, 1177
PspN4I GGNNCC 2 cut(s) 33, 1204
PspPI GGNCC 2 cut(s) 596, 1202
PspXI VCTCGAGB 1 cut(s) 1216
PstI CTGCAG 1 cut(s) 763
PsuI RGATCY 1 cut(s) 286
PsyI GACNNNGTC 1 cut(s) 740
RseI CAYNNNNRTG 1 cut(s) 401
SaqAI TTAA 4 cut(s) 570, 822, 948, 960
SatI GCNGC 9 cut(s) 47, 228, 231, 273, 312, 533, 804, 807, 810
Sau3AI GATC 5 cut(s) 286, 499, 591, 635, 646
Sau96I GGNCC 2 cut(s) 596, 1202
SchI GAGTC 2 cut(s) 118, 750
ScrFI CCNGG 4 cut(s) 10, 615, 1140, 1179
SfaNI GCATC 4 cut(s) 443, 739, 780, 817
SfcI CTRYAG 2 cut(s) 759, 831
Sfr274I CTCGAG 1 cut(s) 1216
SinI GGWCC 1 cut(s) 596
SlaI CTCGAG 1 cut(s) 1216
SmiMI CAYNNNNRTG 1 cut(s) 401
SmlI CTYRAG 3 cut(s) 50, 544, 1216
SmoI CTYRAG 3 cut(s) 50, 544, 1216
SnaBI TACGTA 1 cut(s) 186
Sse9I AATT 8 cut(s) 59, 162, 359, 720, 816, 1028, 1124, 1131
SsiI CCGC 6 cut(s) 35, 231, 273, 803, 806, 1082
StyD4I CCNGG 4 cut(s) 8, 613, 1138, 1177
StyI CCWWGG 3 cut(s) 320, 1193, 1242
TaaI ACNGT 3 cut(s) 31, 101, 394
TaiI ACGT 3 cut(s) 140, 188, 993
TaqI TCGA 3 cut(s) 704, 1172, 1217
TaqII GACCGA 1 cut(s) 584
TasI AATT 8 cut(s) 59, 162, 359, 720, 816, 1028, 1124, 1131
TauI GCSGC 4 cut(s) 233, 275, 806, 809
TfiI GAWTC 3 cut(s) 250, 446, 977
Tru1I TTAA 4 cut(s) 570, 822, 948, 960
Tru9I TTAA 4 cut(s) 570, 822, 948, 960
TseFI GTSAC 3 cut(s) 394, 630, 734
TseI GCWGC 5 cut(s) 46, 227, 311, 532, 809
Tsp45I GTSAC 3 cut(s) 394, 630, 734
TspDTI ATGAA 3 cut(s) 315, 534, 655
TspGWI ACGGA 3 cut(s) 164, 753, 897
Tth111I GACNNNGTC 1 cut(s) 740
VpaK11BI GGWCC 1 cut(s) 596
XagI CCTNNNNNAGG 1 cut(s) 471
XapI RAATTY 1 cut(s) 359
XceI RCATGY 1 cut(s) 1096
XcmI CCANNNNNNNNNTGG 1 cut(s) 646
XhoI CTCGAG 1 cut(s) 1216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.