pycom08g09420

Plant family of unknown function (DUF810)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0013421
Physical Location & Seq
Reverse (-)
7691723 .. 7695408
3686 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g09420.5

Sequence Viewer

Length: 2649 bp
ATGAAGAAGGCTTTGGGTCTCAAATCGCCGGGCTCCGGCACCAAGAAGAGCCCTGGGTTGACCGGGTCCGGGGCCGGGTCGGGTCCCGGGAAGCCCAAAAGGGCTATGACGGTGGGTGAGCTGATGAGGATCCAAATGGGGATTTCTGAGGCCACTGACTCCAGAGTCCGGAGAGCTCTGCTTAGGATTTCTGCTTCACAGGTCGGAAGGCGAATTGAGTCAGTGGTAGTCCCACTGGAACTGCTACAGCAGCTCAAGTGCTCTGATTTCACTGACCAACAAGAATACGATGCATGGCAAAAAAGAACCCTCAAAATTCTTGAGGCCGGTCTCCTCTTGCATCCCCATGTGCCATTAGACAAGTCGAATAATGCTGCACAGAGGTTGCGCCAAGTTATTAACGGGGCATTGGACAGGCCCTTTGAAACCGGTAGAAATAATGAGACTTTGCAGGTTCTTCGTAATGCTGTTACTGCTCTTGCGTCCAGGTCATCCGATGGTCTGTATGATACATCACATTGGGCAGATGGGTTACCATTGAATCTCCGGCTTTATGAGAGGCTTCTAGAAGCCTGCTTTGATCGTCACGACGAAACATCTATAATAGAGGAAGTTGACGAGCTAATGGAGCATATTAAGAAGACCTGGTCAATCCTTGGAATGAATCACATGCTCCATAACCTTTGCTTTACTTGGGTTTTATTTCACCGTTTTGTTGCAACTGGTCAAGTAGAATTGGACCTTTTGTACGCTGCTGATAGTCAGCTAGCTGAAGTTGCTAAAGATGCAAAGGCAACGAAGGATTCCGAGTTCTGCAAGATCTTGAGTTCAACATTGACTTCAATATTGGGTTGGGCAGAGAAAAGACTCCTTGCATATCATGACACTTTTGATAGCAGTAACATTGACACCATGCAGGCAATTGTTTCTCTGGGTGTAGTAGCAGCTAGGATTTTAGTTGAAGATATATCGAATGAGTACCGCAGGAGGAGAAAAAAGGAAGTTGATGTTGCTCGTAGCAGGATTGACACTTATATCAGATCATCGTTACGCACTGCTTTTGCTCAGAGAATGGAAAAGGCAGACTCAAGCAGGAGAGCATCAAGACACCAGCCAAATCCCCTTCCTGTTTTGGCCATCCTTGCGAAGGACGTTGGTGAGCTGGCAGTTAAGGAGAAAGAGGTTTTCAGTCCAATATTGAAGAGATGGCATCCTTTTGCGGCCGGGGTAGCTGTGGCAACCCTTCACGCATGCTATGCGAATGAGATTAAACAATTCATATCGGGTATAGTGGAGTTGACACCAGATGCTGTACAAGTTTTGACAGCTGCAGATAAGCTGGAGAAAGATCTTGTGCTGATAGCAGTTTTAGATTCCGTAGATAGTGATGACGGTGGCAAGGCAATAATTCGTGAGATGCCTCCTTACGAGGCTGAAACTGCAATTGCAAATCTGGTGAAAGTGTGGATTAAGACAAGAGTGGATAGACTGAAAGAATGGATTGATAGGAATCTAGAACAAGAGATATGGAACCCACAAGTAAACAAAGATGGATATGCTCCATCTGCTGTTGAAGTTTTGCGAATCTTTGATGAAACTCTGGAAGCATTCTTTCAGCTGCCAATACCAATGCATCCTGCATTGCTTCCTGACTTGATGACCGGCCTTGATAGATGTCTTCAATACTACGTAACCAAGGCAAAATCTGGATGTGGATCCCGCAATACGTTTGTTCCCACTATGCCAGCATTGACCAGATGTACCATCGGATCAAAGTTCCAAGGATTTGGCAAAAAGAAGGAAAAATCACCAGTTCCTCAGAAGAGGAACTCTCTGGTTGCGACACTCAATGGGGATAACTCCTTTGGGATACCACAGATGTGTGTTTGTATAAACACATTGCAAAGAATCCGGAGTGAGTTGGAGGTTTTGGAGAAACGGATTATTACTCATCTCAGAAATTCAAAATCTGCTAATGAAGAAGACTTCTCGAATGGACTGGGGAAAAAGTTTGAACTCACACCTGCAGCTTGTGTGGTAGCAATCCAACAGCTTTGTGAGGCAGTGGCTTATAAAATGATCTTCCATGACTTAAGTCATGTTCTGTGGGATGGTTTGTACGTTGGGGAACCATCCTTTTGTAGGATAGACGCTTTTCTTGACGAGCTGGAGAAGAATTTACTCATCATATCCAACACTGTGCACGAAAGAGTTCGAACACGGATTATTACTGACATAATGAGAGCGTCATTTGATGGATTCTTACTGGTTTTGCTTGCTGGAGGCCCCTCTCGTGCATTCTCTCGGCAGGATTCCCAAATAATAGAGGATGATTTCAAAGCCCTCAAAGATCTATTCTGGGCCAATGGGGATGGCTTGCCTTCTGAATTAATAGACAAGTTTTCAACAACTGTGAGAGGTGTGCTTCCCCTTTTCAAAACCGATACTGAGAGTCTTGTTGAACGGTTTAGACGTGTGACCTTGGAGTCATATGGCTCGTCCGCCAGGTCCAGACTCCCGTTGCCCCCAACTTCCGGGCAGTGGAACCCAACTGAACCAAACACGCTCCTCCGGGTGTTGTGCTACAGAAATGATGAAGCAGCCACCAAGTTTCTTAAGAAGACTTATAATCTGCCCAAGAAACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF810

Protein Analysis

883

Amino Acids

98.88

Weight (kDa)

8.62

Isoelectric Point (pI)

45.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013421)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25800
fragaria_vesca FvH4_2g35590 FvH4_2g35590
malus_domestica MD08G1113600.v1.1 MD15G1093300.v1.1
prunus_persica Prupe.1G448100_v2.0.a1
pyrus_communis pycom08g09420 pycom15g08610
rosa_chinensis RchiOBHm_Chr6g0306221
rosa_laevigata RLG00000010812
rosa_multiflora Rmu_sc0003594.1_g000002
rosa_roxburghii Rroxscaffold_7G00161920
rosa_rugosa Rorug06G0348100
rosa_samantha Rh6AG460700 Rh6BG440700 Rh6CG474700 Rh6DG461000
rosa_wichuraiana Rw6G040130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2073, 2628
AarI CACCTGC 1 cut(s) 2032
AasI GACNNNNNNGTC 2 cut(s) 164, 2485
Acc36I ACCTGC 2 cut(s) 442, 2032
AccB1I GGYRCC 1 cut(s) 38
AccIII TCCGGA 2 cut(s) 168, 1911
AciI CCGC 4 cut(s) 982, 1220, 1720, 2502
AclWI GGATC 5 cut(s) 124, 137, 1710, 1723, 1777
AcoI YGGCCR 2 cut(s) 1134, 1221
AcsI RAATTY 3 cut(s) 315, 1960, 2176
AcuI CTGAAG 1 cut(s) 792
AfaI GTAC 5 cut(s) 749, 980, 1314, 1762, 2120
AfiI CCNNNNNNNGG 8 cut(s) 35, 69, 75, 168, 1147, 2142, 2534, 2541
AflII CTTAAG 2 cut(s) 2092, 2615
AflIII ACRYGT 1 cut(s) 2473
AgeI ACCGGT 1 cut(s) 428
AjiI CACGTC 1 cut(s) 2474
AjnI CCWGG 4 cut(s) 52, 485, 644, 2504
AjuI GAANNNNNNNTTGG 1 cut(s) 28
AleI CACNNNNGTG 1 cut(s) 1879
AloI GAACNNNNNNTCC 2 cut(s) 794, 826
Alw21I GWGCWC 3 cut(s) 178, 263, 2205
Alw26I GTCTC 3 cut(s) 23, 335, 437
Alw44I GTGCAC 1 cut(s) 2201
AlwI GGATC 5 cut(s) 124, 137, 1710, 1723, 1777
AlwNI CAGNNNCTG 1 cut(s) 1310
Ama87I CYCGRG 1 cut(s) 86
Aor13HI TCCGGA 2 cut(s) 168, 1911
AoxI GGCC 9 cut(s) 72, 150, 324, 416, 1134, 1221, 1663, 2284, 2361
ApaLI GTGCAC 1 cut(s) 2201
ApeKI GCWGC 8 cut(s) 250, 374, 752, 944, 1328, 1618, 2027, 2600
ApoI RAATTY 3 cut(s) 315, 1960, 2176
ArsI GACNNNNNNTTYG 2 cut(s) 404, 436
AseI ATTAAT 1 cut(s) 2390
AsiGI ACCGGT 1 cut(s) 428
Asp700I GAANNNNTTC 1 cut(s) 2211
AspLEI GCGC 1 cut(s) 390
AspS9I GGNCC 8 cut(s) 66, 72, 83, 417, 739, 2285, 2361, 2508
AsuC2I CCSGG 9 cut(s) 30, 64, 70, 76, 87, 88, 1225, 2536, 2573
AsuHPI GGTGA 5 cut(s) 128, 698, 1169, 1468, 1800
AsuII TTCGAA 1 cut(s) 2215
AsuNHI GCTAGC 1 cut(s) 766
AvaI CYCGRG 1 cut(s) 86
AvaII GGWCC 4 cut(s) 66, 83, 739, 2508
BaeGI GKGCMC 1 cut(s) 2205
BalI TGGCCA 1 cut(s) 1136
BamHI GGATCC 2 cut(s) 129, 1715
BanI GGYRCC 1 cut(s) 38
BanII GRGCYC 3 cut(s) 35, 53, 178
BauI CACGAG 1 cut(s) 2292
BbsI GAAGAC 4 cut(s) 647, 1670, 1989, 2627
Bbv12I GWGCWC 3 cut(s) 178, 263, 2205
BbvI GCAGC 8 cut(s) 262, 361, 739, 956, 1315, 1605, 2039, 2612
BcgI CGANNNNNNTGC 2 cut(s) 440, 474
BciT130I CCWGG 4 cut(s) 54, 487, 646, 2506
BciVI GTATCC 1 cut(s) 1863
BcnI CCSGG 9 cut(s) 30, 64, 70, 76, 87, 88, 1225, 2536, 2573
BcoDI GTCTC 3 cut(s) 23, 335, 437
BfaI CTAG 4 cut(s) 566, 767, 948, 1514
BfmI CTRYAG 4 cut(s) 245, 1329, 2025, 2584
BfrI CTTAAG 2 cut(s) 2092, 2615
BfuAI ACCTGC 2 cut(s) 442, 2032
BfuI GTATCC 1 cut(s) 1863
BglII AGATCT 3 cut(s) 819, 1348, 2350
BisI GCNGC 9 cut(s) 251, 375, 753, 945, 1221, 1329, 1619, 2028, 2601
BlsI GCNGC 9 cut(s) 252, 376, 754, 946, 1222, 1330, 1620, 2029, 2602
Bme18I GGWCC 4 cut(s) 66, 83, 739, 2508
BmeT110I CYCGRG 1 cut(s) 86
BmgBI CACGTC 1 cut(s) 2474
BmgT120I GGNCC 8 cut(s) 66, 72, 83, 417, 739, 2285, 2361, 2508
BmrI ACTGGG 1 cut(s) 2009
BmsI GCATC 8 cut(s) 280, 349, 775, 1109, 1219, 1297, 1407, 1642
BmtI GCTAGC 1 cut(s) 770
BmuI ACTGGG 1 cut(s) 2009
BoxI GACNNNNGTC 1 cut(s) 2094
BpiI GAAGAC 4 cut(s) 647, 1670, 1989, 2627
BplI GAGNNNNNCTC 2 cut(s) 1816, 1848
BpmI CTGGAG 4 cut(s) 145, 1361, 2189, 2301
Bpu10I CCTNAGC 1 cut(s) 182
Bpu14I TTCGAA 1 cut(s) 2215
BpuEI CTTGAG 4 cut(s) 239, 341, 844, 1072
BpuMI CCSGG 9 cut(s) 30, 64, 70, 76, 87, 88, 1225, 2536, 2573
BsaAI YACGTR 1 cut(s) 1690
BsaBI GATNNNNATC 3 cut(s) 128, 1509, 1714
BsaI GGTCTC 2 cut(s) 23, 335
BsaJI CCNNGG 9 cut(s) 52, 53, 69, 86, 655, 1224, 1695, 1780, 2481
BsaWI WCCGGW 3 cut(s) 168, 428, 1911
Bsc4I CCNNNNNNNGG 8 cut(s) 35, 69, 75, 168, 1147, 2142, 2534, 2541
Bse118I RCCGGY 3 cut(s) 326, 428, 1661
Bse1I ACTGG 5 cut(s) 240, 727, 1811, 2004, 2271
Bse3DI GCAATG 2 cut(s) 1640, 1898
Bse8I GATNNNNATC 3 cut(s) 128, 1509, 1714
BseAI TCCGGA 2 cut(s) 168, 1911
BseBI CCWGG 4 cut(s) 54, 487, 646, 2506
BseDI CCNNGG 9 cut(s) 52, 53, 69, 86, 655, 1224, 1695, 1780, 2481
BseJI GATNNNNATC 3 cut(s) 128, 1509, 1714
BseLI CCNNNNNNNGG 8 cut(s) 35, 69, 75, 168, 1147, 2142, 2534, 2541
BseMI GCAATG 2 cut(s) 1640, 1898
BseMII CTCAG 5 cut(s) 138, 1079, 1832, 1969, 2439
BseNI ACTGG 5 cut(s) 240, 727, 1811, 2004, 2271
BseRI GAGGAG 3 cut(s) 323, 1003, 2558
BseSI GKGCMC 1 cut(s) 2205
BseX3I CGGCCG 1 cut(s) 1221
BseXI GCAGC 8 cut(s) 262, 361, 739, 956, 1315, 1605, 2039, 2612
BsgI GTGCAG 1 cut(s) 360
Bsh1285I CGRYCG 1 cut(s) 1224
BshFI GGCC 9 cut(s) 74, 152, 326, 418, 1136, 1223, 1665, 2286, 2363
BshNI GGYRCC 1 cut(s) 38
BshTI ACCGGT 1 cut(s) 428
BsiEI CGRYCG 1 cut(s) 1224
BsiHKAI GWGCWC 3 cut(s) 178, 263, 2205
BsiHKCI CYCGRG 1 cut(s) 86
BslFI GGGAC 2 cut(s) 69, 215
BslI CCNNNNNNNGG 8 cut(s) 35, 69, 75, 168, 1147, 2142, 2534, 2541
BsmAI GTCTC 3 cut(s) 23, 335, 437
BsmFI GGGAC 2 cut(s) 69, 215
BsmI GAATGC 2 cut(s) 1607, 2297
BsnI GGCC 9 cut(s) 74, 152, 326, 418, 1136, 1223, 1665, 2286, 2363
Bso31I GGTCTC 2 cut(s) 23, 335
BsoBI CYCGRG 1 cut(s) 86
Bsp119I TTCGAA 1 cut(s) 2215
Bsp1286I GDGCHC 5 cut(s) 35, 53, 178, 263, 2205
Bsp13I TCCGGA 2 cut(s) 168, 1911
Bsp1407I TGTACA 1 cut(s) 1312
Bsp143I GATC 9 cut(s) 129, 580, 819, 1040, 1348, 1715, 1769, 2079, 2350
BspACI CCGC 4 cut(s) 982, 1220, 1720, 2502
BspANI GGCC 9 cut(s) 74, 152, 326, 418, 1136, 1223, 1665, 2286, 2363
BspCNI CTCAG 5 cut(s) 139, 1078, 1831, 1968, 2440
BspEI TCCGGA 2 cut(s) 168, 1911
BspHI TCATGA 1 cut(s) 880
BspMAI CTGCAG 2 cut(s) 1333, 2029
BspMI ACCTGC 2 cut(s) 442, 2032
BspOI GCTAGC 1 cut(s) 770
BspPI GGATC 5 cut(s) 124, 137, 1710, 1723, 1777
BspQI GCTCTTC 1 cut(s) 41
BspT104I TTCGAA 1 cut(s) 2215
BspT107I GGYRCC 1 cut(s) 38
BspTI CTTAAG 2 cut(s) 2092, 2615
BspTNI GGTCTC 2 cut(s) 23, 335
BsrDI GCAATG 2 cut(s) 1640, 1898
BsrFI RCCGGY 3 cut(s) 326, 428, 1661
BsrGI TGTACA 1 cut(s) 1312
BsrI ACTGG 5 cut(s) 240, 727, 1811, 2004, 2271
BssAI RCCGGY 3 cut(s) 326, 428, 1661
BssECI CCNNGG 9 cut(s) 52, 53, 69, 86, 655, 1224, 1695, 1780, 2481
BssMI GATC 9 cut(s) 129, 580, 819, 1040, 1348, 1715, 1769, 2079, 2350
BssSI CACGAG 1 cut(s) 2292
BssT1I CCWWGG 4 cut(s) 655, 1695, 1780, 2481
Bst2BI CACGAG 1 cut(s) 2292
Bst2UI CCWGG 4 cut(s) 54, 487, 646, 2506
Bst4CI ACNGT 7 cut(s) 112, 710, 1394, 2200, 2413, 2466, 2646
Bst6I CTCTTC 3 cut(s) 41, 1196, 1817
BstAFI CTTAAG 2 cut(s) 2092, 2615
BstAPI GCANNNNNTGC 1 cut(s) 1256
BstAUI TGTACA 1 cut(s) 1312
BstBAI YACGTR 1 cut(s) 1690
BstBI TTCGAA 1 cut(s) 2215
BstC8I GCNNGC 8 cut(s) 574, 768, 918, 1164, 1252, 1746, 2277, 2378
BstDEI CTNAG 6 cut(s) 147, 182, 1065, 1818, 1955, 2448
BstEII GGTNACC 1 cut(s) 531
BstENI CCTNNNNNAGG 2 cut(s) 1145, 2140
BstHHI GCGC 1 cut(s) 390
BstKTI GATC 9 cut(s) 132, 583, 822, 1043, 1351, 1718, 1772, 2082, 2353
BstMAI GTCTC 3 cut(s) 23, 335, 437
BstMBI GATC 9 cut(s) 129, 580, 819, 1040, 1348, 1715, 1769, 2079, 2350
BstMCI CGRYCG 1 cut(s) 1224
BstNI CCWGG 4 cut(s) 54, 487, 646, 2506
BstNSI RCATGY 2 cut(s) 673, 1254
BstPAI GACNNNNGTC 1 cut(s) 2094
BstPI GGTNACC 1 cut(s) 531
BstSFI CTRYAG 4 cut(s) 245, 1329, 2025, 2584
BstSLI GKGCMC 1 cut(s) 2205
BstSNI TACGTA 1 cut(s) 1690
BstV1I GCAGC 8 cut(s) 262, 361, 739, 956, 1315, 1605, 2039, 2612
BstV2I GAAGAC 4 cut(s) 647, 1670, 1989, 2627
BstX2I RGATCY 5 cut(s) 129, 819, 1348, 1715, 2350
BstXI CCANNNNNNTGG 1 cut(s) 1787
BstYI RGATCY 5 cut(s) 129, 819, 1348, 1715, 2350
BstZI CGGCCG 1 cut(s) 1221
BsuI GTATCC 1 cut(s) 1863
BsuRI GGCC 9 cut(s) 74, 152, 326, 418, 1136, 1223, 1665, 2286, 2363
BtrI CACGTC 1 cut(s) 2474
BtsI GCAGTG 3 cut(s) 1053, 2070, 2546
BtsIMutI CAGTG 8 cut(s) 153, 228, 233, 270, 1053, 2070, 2196, 2546
BveI ACCTGC 2 cut(s) 442, 2032
Cac8I GCNNGC 8 cut(s) 574, 768, 918, 1164, 1252, 1746, 2277, 2378
CaiI CAGNNNCTG 1 cut(s) 1310
CciI TCATGA 1 cut(s) 880
CfoI GCGC 1 cut(s) 390
Cfr10I RCCGGY 3 cut(s) 326, 428, 1661
Cfr13I GGNCC 8 cut(s) 66, 72, 83, 417, 739, 2285, 2361, 2508
Cfr9I CCCGGG 1 cut(s) 86
CseI GACGC 3 cut(s) 471, 2159, 2235
CsiI ACCWGGT 1 cut(s) 644
Csp6I GTAC 5 cut(s) 748, 979, 1313, 1761, 2119
CspAI ACCGGT 1 cut(s) 428
CviAII CATG 8 cut(s) 294, 347, 670, 881, 913, 1251, 2087, 2099
CviQI GTAC 5 cut(s) 748, 979, 1313, 1761, 2119
DdeI CTNAG 6 cut(s) 147, 182, 1065, 1818, 1955, 2448
DpnI GATC 9 cut(s) 131, 582, 821, 1042, 1350, 1717, 1771, 2081, 2352
DpnII GATC 9 cut(s) 129, 580, 819, 1040, 1348, 1715, 1769, 2079, 2350
DrdI GACNNNNNNGTC 2 cut(s) 164, 2485
DseDI GACNNNNNNGTC 2 cut(s) 164, 2485
EaeI YGGCCR 2 cut(s) 1134, 1221
EagI CGGCCG 1 cut(s) 1221
Eam1104I CTCTTC 3 cut(s) 41, 1196, 1817
EarI CTCTTC 3 cut(s) 41, 1196, 1817
EciI GGCGGA 1 cut(s) 2491
Ecl136II GAGCTC 1 cut(s) 176
EclXI CGGCCG 1 cut(s) 1221
Eco105I TACGTA 1 cut(s) 1690
Eco130I CCWWGG 4 cut(s) 655, 1695, 1780, 2481
Eco24I GRGCYC 3 cut(s) 35, 53, 178
Eco31I GGTCTC 2 cut(s) 23, 335
Eco47I GGWCC 4 cut(s) 66, 83, 739, 2508
Eco52I CGGCCG 1 cut(s) 1221
Eco53kI GAGCTC 1 cut(s) 176
Eco57I CTGAAG 1 cut(s) 792
Eco88I CYCGRG 1 cut(s) 86
Eco91I GGTNACC 1 cut(s) 531
EcoICRI GAGCTC 1 cut(s) 176
EcoNI CCTNNNNNAGG 2 cut(s) 1145, 2140
EcoO109I RGGNCCY 3 cut(s) 83, 417, 2285
EcoO65I GGTNACC 1 cut(s) 531
EcoRII CCWGG 4 cut(s) 52, 485, 644, 2504
EcoT14I CCWWGG 4 cut(s) 655, 1695, 1780, 2481
EcoT22I ATGCAT 2 cut(s) 295, 1635
EcoT38I GRGCYC 3 cut(s) 35, 53, 178
ErhI CCWWGG 4 cut(s) 655, 1695, 1780, 2481
FaeI CATG 8 cut(s) 297, 350, 673, 884, 916, 1254, 2090, 2102
FaqI GGGAC 2 cut(s) 69, 215
FatI CATG 8 cut(s) 293, 346, 669, 880, 912, 1250, 2086, 2098
FauI CCCGC 1 cut(s) 1727
FauNDI CATATG 1 cut(s) 2491
Fnu4HI GCNGC 9 cut(s) 251, 375, 753, 945, 1221, 1329, 1619, 2028, 2601
FriOI GRGCYC 3 cut(s) 35, 53, 178
Fsp4HI GCNGC 9 cut(s) 251, 375, 753, 945, 1221, 1329, 1619, 2028, 2601
FspBI CTAG 4 cut(s) 566, 767, 948, 1514
GlaI GCGC 1 cut(s) 389
GluI GCNGC 9 cut(s) 251, 375, 753, 945, 1221, 1329, 1619, 2028, 2601
GsuI CTGGAG 4 cut(s) 145, 1361, 2189, 2301
HaeIII GGCC 9 cut(s) 74, 152, 326, 418, 1136, 1223, 1665, 2286, 2363
HgaI GACGC 3 cut(s) 471, 2159, 2235
HhaI GCGC 1 cut(s) 390
Hin1II CATG 8 cut(s) 297, 350, 673, 884, 916, 1254, 2090, 2102
Hin6I GCGC 1 cut(s) 388
HinP1I GCGC 1 cut(s) 388
HincII GTYRAC 3 cut(s) 60, 616, 1299
HindII GTYRAC 3 cut(s) 60, 616, 1299
HphI GGTGA 5 cut(s) 128, 698, 1169, 1468, 1800
Hpy166II GTNNAC 5 cut(s) 60, 616, 1299, 1543, 2203
Hpy8I GTNNAC 5 cut(s) 60, 616, 1299, 1543, 2203
Hpy99I CGWCG 1 cut(s) 593
HpyAV CCTTC 7 cut(s) 201, 793, 1133, 1141, 1253, 1792, 2391
HpyCH4III ACNGT 7 cut(s) 112, 710, 1394, 2200, 2413, 2466, 2646
HpyCH4IV ACGT 5 cut(s) 1152, 1689, 1727, 2121, 2473
HpyF3I CTNAG 6 cut(s) 147, 182, 1065, 1818, 1955, 2448
HpySE526I ACGT 5 cut(s) 1152, 1689, 1727, 2121, 2473
Hsp92II CATG 8 cut(s) 297, 350, 673, 884, 916, 1254, 2090, 2102
HspAI GCGC 1 cut(s) 388
KflI GGGWCCC 1 cut(s) 83
Kpn2I TCCGGA 2 cut(s) 168, 1911
Kzo9I GATC 9 cut(s) 129, 580, 819, 1040, 1348, 1715, 1769, 2079, 2350
LguI GCTCTTC 1 cut(s) 41
LmnI GCTCC 5 cut(s) 38, 628, 678, 1564, 2571
Lsp1109I GCAGC 8 cut(s) 262, 361, 739, 956, 1315, 1605, 2039, 2612
LweI GCATC 8 cut(s) 280, 349, 775, 1109, 1219, 1297, 1407, 1642
MabI ACCWGGT 1 cut(s) 644
MaeI CTAG 4 cut(s) 566, 767, 948, 1514
MaeII ACGT 5 cut(s) 1152, 1689, 1727, 2121, 2473
MaeIII GTNAC 7 cut(s) 469, 531, 584, 899, 1047, 1690, 2476
MalI GATC 9 cut(s) 131, 582, 821, 1042, 1350, 1717, 1771, 2081, 2352
MboI GATC 9 cut(s) 129, 580, 819, 1040, 1348, 1715, 1769, 2079, 2350
MfeI CAATTG 2 cut(s) 921, 1443
MflI RGATCY 5 cut(s) 129, 819, 1348, 1715, 2350
MhlI GDGCHC 5 cut(s) 35, 53, 178, 263, 2205
MlsI TGGCCA 1 cut(s) 1136
MluNI TGGCCA 1 cut(s) 1136
MlyI GAGTC 8 cut(s) 152, 174, 227, 861, 1079, 2461, 2495, 2508
MmeI TCCRAC 4 cut(s) 184, 1902, 2071, 2217
Mox20I TGGCCA 1 cut(s) 1136
Mph1103I ATGCAT 2 cut(s) 295, 1635
MroI TCCGGA 2 cut(s) 168, 1911
MroXI GAANNNNTTC 1 cut(s) 2211
MscI TGGCCA 1 cut(s) 1136
MseI TTAA 8 cut(s) 399, 636, 1170, 1269, 1470, 2093, 2390, 2616
MslI CAYNNNNRTG 2 cut(s) 345, 1879
Msp20I TGGCCA 1 cut(s) 1136
MspA1I CMGCKG 2 cut(s) 1328, 1618
MspCI CTTAAG 2 cut(s) 2092, 2615
MunI CAATTG 2 cut(s) 921, 1443
Mva1269I GAATGC 2 cut(s) 1607, 2297
MvaI CCWGG 4 cut(s) 54, 487, 646, 2506
NciI CCSGG 9 cut(s) 30, 64, 70, 76, 87, 88, 1225, 2536, 2573
NdeI CATATG 1 cut(s) 2491
NdeII GATC 9 cut(s) 129, 580, 819, 1040, 1348, 1715, 1769, 2079, 2350
NheI GCTAGC 1 cut(s) 766
NlaIII CATG 8 cut(s) 297, 350, 673, 884, 916, 1254, 2090, 2102
NmeAIII GCCGAG 1 cut(s) 2284
NmuCI GTSAC 2 cut(s) 584, 2476
NsiI ATGCAT 2 cut(s) 295, 1635
NspI RCATGY 2 cut(s) 673, 1254
NspV TTCGAA 1 cut(s) 2215
OliI CACNNNNGTG 1 cut(s) 1879
PaeI GCATGC 1 cut(s) 1254
PagI TCATGA 1 cut(s) 880
PaqCI CACCTGC 1 cut(s) 2032
PasI CCCWGGG 1 cut(s) 53
PciSI GCTCTTC 1 cut(s) 41
PcsI WCGNNNNNNNCGW 1 cut(s) 2470
PctI GAATGC 2 cut(s) 1607, 2297
PdmI GAANNNNTTC 1 cut(s) 2211
PfeI GAWTC 9 cut(s) 541, 664, 803, 1373, 1510, 1584, 1908, 2259, 2312
PflFI GACNNNGTC 2 cut(s) 64, 646
PinAI ACCGGT 1 cut(s) 428
PkrI GCNGC 9 cut(s) 252, 376, 754, 946, 1222, 1330, 1620, 2029, 2602
PleI GAGTC 8 cut(s) 152, 173, 226, 861, 1079, 2460, 2494, 2508
PpsI GAGTC 8 cut(s) 152, 173, 226, 861, 1079, 2460, 2494, 2508
Ppu21I YACGTR 1 cut(s) 1690
PpuMI RGGWCCY 1 cut(s) 83
PshAI GACNNNNGTC 1 cut(s) 2094
PshBI ATTAAT 1 cut(s) 2390
PsiI TTATAA 2 cut(s) 2073, 2628
Psp124BI GAGCTC 1 cut(s) 178
Psp5II RGGWCCY 1 cut(s) 83
Psp6I CCWGG 4 cut(s) 52, 485, 644, 2504
PspEI GGTNACC 1 cut(s) 531
PspGI CCWGG 4 cut(s) 52, 485, 644, 2504
PspPI GGNCC 8 cut(s) 66, 72, 83, 417, 739, 2285, 2361, 2508
PspPPI RGGWCCY 1 cut(s) 83
PstI CTGCAG 2 cut(s) 1333, 2029
PstNI CAGNNNCTG 1 cut(s) 1310
PsuI RGATCY 5 cut(s) 129, 819, 1348, 1715, 2350
PsyI GACNNNGTC 2 cut(s) 64, 646
PvuII CAGCTG 2 cut(s) 1328, 1618
RsaI GTAC 5 cut(s) 749, 980, 1314, 1762, 2120
RsaNI GTAC 5 cut(s) 748, 979, 1313, 1761, 2119
RseI CAYNNNNRTG 2 cut(s) 345, 1879
SacI GAGCTC 1 cut(s) 178
SapI GCTCTTC 1 cut(s) 41
SaqAI TTAA 8 cut(s) 399, 636, 1170, 1269, 1470, 2093, 2390, 2616
SatI GCNGC 9 cut(s) 251, 375, 753, 945, 1221, 1329, 1619, 2028, 2601
Sau3AI GATC 9 cut(s) 129, 580, 819, 1040, 1348, 1715, 1769, 2079, 2350
Sau96I GGNCC 8 cut(s) 66, 72, 83, 417, 739, 2285, 2361, 2508
SchI GAGTC 8 cut(s) 152, 174, 227, 861, 1079, 2461, 2495, 2508
SduI GDGCHC 5 cut(s) 35, 53, 178, 263, 2205
SexAI ACCWGGT 1 cut(s) 644
SfaNI GCATC 8 cut(s) 280, 349, 775, 1109, 1219, 1297, 1407, 1642
SfcI CTRYAG 4 cut(s) 245, 1329, 2025, 2584
SfuI TTCGAA 1 cut(s) 2215
SinI GGWCC 4 cut(s) 66, 83, 739, 2508
SmaI CCCGGG 1 cut(s) 88
SmiMI CAYNNNNRTG 2 cut(s) 345, 1879
SmlI CTYRAG 6 cut(s) 254, 320, 823, 1087, 2092, 2615
SmoI CTYRAG 6 cut(s) 254, 320, 823, 1087, 2092, 2615
SnaBI TACGTA 1 cut(s) 1690
SphI GCATGC 1 cut(s) 1254
SsiI CCGC 4 cut(s) 982, 1220, 1720, 2502
SspI AATATT 2 cut(s) 846, 1197
SspMI CTAG 4 cut(s) 566, 767, 948, 1514
SstI GAGCTC 1 cut(s) 178
StyI CCWWGG 4 cut(s) 655, 1695, 1780, 2481
TaaI ACNGT 7 cut(s) 112, 710, 1394, 2200, 2413, 2466, 2646
TaiI ACGT 5 cut(s) 1155, 1692, 1730, 2124, 2476
TaqI TCGA 4 cut(s) 365, 971, 1991, 2215
TatI WGTACW 1 cut(s) 1312
TauI GCSGC 1 cut(s) 1223
TfiI GAWTC 9 cut(s) 541, 664, 803, 1373, 1510, 1584, 1908, 2259, 2312
Tru1I TTAA 8 cut(s) 399, 636, 1170, 1269, 1470, 2093, 2390, 2616
Tru9I TTAA 8 cut(s) 399, 636, 1170, 1269, 1470, 2093, 2390, 2616
TscAI CASTG 8 cut(s) 160, 228, 240, 277, 1060, 2070, 2203, 2546
TseFI GTSAC 2 cut(s) 584, 2476
TseI GCWGC 8 cut(s) 250, 374, 752, 944, 1328, 1618, 2027, 2600
Tsp45I GTSAC 2 cut(s) 584, 2476
TspDTI ATGAA 6 cut(s) 17, 677, 1267, 1608, 1992, 2610
TspGWI ACGGA 3 cut(s) 1366, 1954, 2236
TspMI CCCGGG 1 cut(s) 86
TspRI CASTG 8 cut(s) 160, 228, 240, 277, 1060, 2070, 2203, 2546
Tth111I GACNNNGTC 2 cut(s) 64, 646
Vha464I CTTAAG 2 cut(s) 2092, 2615
VneI GTGCAC 1 cut(s) 2201
VpaK11BI GGWCC 4 cut(s) 66, 83, 739, 2508
VspI ATTAAT 1 cut(s) 2390
XagI CCTNNNNNAGG 2 cut(s) 1145, 2140
XapI RAATTY 3 cut(s) 315, 1960, 2176
XbaI TCTAGA 2 cut(s) 565, 1513
XceI RCATGY 2 cut(s) 673, 1254
XmaI CCCGGG 1 cut(s) 86
XmnI GAANNNNTTC 1 cut(s) 2211
XspI CTAG 4 cut(s) 566, 767, 948, 1514
Zsp2I ATGCAT 2 cut(s) 295, 1635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.