pycom08g10300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0015560
Physical Location & Seq
Forward (+)
8657290 .. 8658626
1337 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g10300.3

Sequence Viewer

Length: 666 bp
ATGAAGCGCAAAGACTTTGTAGAATTAGACGACGACGACTACTTCTCTCCTTTCTCAAAATCCAGGAGATTGGATGGTGGGTTGTTTGCAACGGTAAATGAAGACCAAAGTAGTGCAGCTCAGGTGTTTGAAGAAATGCCGACACCAGAAACCAGTGTTGTGATGCAAACAGATGATTTGCCAACGGAACCTTTGCCTTCTGGGGATGAGAAAGCTCTTGTGCTCTATAACCCCGCGAATACGCCGTTTTTCAAAGCACCTGATTCGCGGGATTTCTCTGTCATTGTGAATTCGGACTTGATTCCGGGTTTAAGAGATCATATTTATTCGTGGGGATATTCAAAGTCACTGAAGCCGGTAGAGGATCGTGGGTCGGAAGAGGAAAACAAAGATGTTTCAAATGGGTGTATGGCTGTTGTTCCCTGGGTTGCACCCAACTTCCCACCGTCTTCACGAGATGTGACACAGACTGCAAGTCAGTCGGAGCCCATGGAAGTTGAAATGATGGACACAGATGATAACAGATTCAATGGGGCGGAAGCGTCTGGGTTCAGTGGAACGATGATGGAAGGATCGAGTGGGGTACAAAACTGGCATCAGCAACAGCAGCTGCATTGGATGGAGCCACAACTCCTCCTGAACAATTATACTCCCGTCACTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

222

Amino Acids

24.8

Weight (kDa)

4.26

Isoelectric Point (pI)

52.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015560)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g36450
malus_domestica MD08G1124200.v1.1 MD15G1102900.v1.1
prunus_persica Prupe.1G455900_v2.0.a1
pyrus_communis pycom08g10300 pycom15g09390
rosa_chinensis RchiOBHm_Chr6g0305451
rosa_laevigata RLG00000010891
rosa_multiflora Rmu_sc0007817.1_g000006
rosa_roxburghii Rroxscaffold_7G00162810
rosa_rugosa Rorug06G0340300
rosa_samantha Rh6AG452400 Rh6BG449400 Rh6CG466100 Rh6DG452800
rosa_wichuraiana Rw6G039410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 236, 268
AciI CCGC 3 cut(s) 234, 268, 536
AclWI GGATC 2 cut(s) 372, 580
AcsI RAATTY 1 cut(s) 289
AcuI CTGAAG 1 cut(s) 371
AfaI GTAC 1 cut(s) 585
AfiI CCNNNNNNNGG 1 cut(s) 660
AgsI TTSAA 6 cut(s) 131, 253, 342, 399, 500, 529
AhdI GACNNNNNGTC 1 cut(s) 474
AjnI CCWGG 2 cut(s) 62, 422
AluBI AGCT 3 cut(s) 119, 215, 610
AluI AGCT 3 cut(s) 119, 215, 610
Alw21I GWGCWC 1 cut(s) 225
AlwI GGATC 2 cut(s) 372, 580
AlwNI CAGNNNCTG 1 cut(s) 610
ApeKI GCWGC 3 cut(s) 116, 607, 610
ApoI RAATTY 1 cut(s) 289
AspLEI GCGC 1 cut(s) 9
AsuC2I CCSGG 1 cut(s) 306
BanII GRGCYC 1 cut(s) 489
BauI CACGAG 1 cut(s) 453
BbsI GAAGAC 2 cut(s) 108, 441
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 3 cut(s) 128, 597, 619
BccI CCATC 4 cut(s) 68, 499, 559, 613
BceAI ACGGC 1 cut(s) 229
BcgI CGANNNNNNTGC 4 cut(s) 246, 280, 462, 496
BciT130I CCWGG 2 cut(s) 64, 424
BcnI CCSGG 1 cut(s) 306
BisI GCNGC 3 cut(s) 117, 608, 611
BlsI GCNGC 3 cut(s) 118, 609, 612
Bme1390I CCNGG 3 cut(s) 64, 306, 424
BmeRI GACNNNNNGTC 1 cut(s) 474
BmiI GGNNCC 3 cut(s) 189, 486, 624
BmrFI CCNGG 3 cut(s) 64, 306, 424
BmsI GCATC 2 cut(s) 153, 604
BpiI GAAGAC 2 cut(s) 108, 441
Bpu10I CCTNAGC 1 cut(s) 120
BpuMI CCSGG 1 cut(s) 306
BsaJI CCNNGG 3 cut(s) 422, 423, 489
BsaXI ACNNNNNCTCC 2 cut(s) 618, 648
Bsc4I CCNNNNNNNGG 1 cut(s) 660
Bse118I RCCGGY 1 cut(s) 355
Bse1I ACTGG 2 cut(s) 153, 596
BseBI CCWGG 2 cut(s) 64, 424
BseDI CCNNGG 3 cut(s) 422, 423, 489
BseGI GGATG 3 cut(s) 79, 211, 624
BseLI CCNNNNNNNGG 1 cut(s) 660
BseMII CTCAG 1 cut(s) 134
BseNI ACTGG 2 cut(s) 153, 596
BseRI GAGGAG 1 cut(s) 623
BseXI GCAGC 3 cut(s) 128, 597, 619
BsgI GTGCAG 1 cut(s) 135
Bsh1236I CGCG 2 cut(s) 236, 268
BsiHKAI GWGCWC 1 cut(s) 225
BsiSI CCGG 2 cut(s) 305, 356
BslI CCNNNNNNNGG 1 cut(s) 660
Bsp1286I GDGCHC 2 cut(s) 225, 489
Bsp143I GATC 3 cut(s) 316, 364, 572
Bsp19I CCATGG 1 cut(s) 489
BspACI CCGC 3 cut(s) 234, 268, 536
BspCNI CTCAG 1 cut(s) 133
BspFNI CGCG 2 cut(s) 236, 268
BspLI GGNNCC 3 cut(s) 189, 486, 624
BspPI GGATC 2 cut(s) 372, 580
BsrFI RCCGGY 1 cut(s) 355
BsrI ACTGG 2 cut(s) 153, 596
BssAI RCCGGY 1 cut(s) 355
BssECI CCNNGG 3 cut(s) 422, 423, 489
BssMI GATC 3 cut(s) 316, 364, 572
BssSI CACGAG 1 cut(s) 453
BssT1I CCWWGG 1 cut(s) 489
Bst2BI CACGAG 1 cut(s) 453
Bst2UI CCWGG 2 cut(s) 64, 424
Bst4CI ACNGT 2 cut(s) 94, 447
Bst6I CTCTTC 1 cut(s) 372
BstDEI CTNAG 1 cut(s) 120
BstDSI CCRYGG 1 cut(s) 489
BstF5I GGATG 3 cut(s) 79, 211, 624
BstFNI CGCG 2 cut(s) 236, 268
BstHHI GCGC 1 cut(s) 9
BstKTI GATC 3 cut(s) 319, 367, 575
BstMBI GATC 3 cut(s) 316, 364, 572
BstMWI GCNNNNNNNGC 1 cut(s) 607
BstNI CCWGG 2 cut(s) 64, 424
BstSCI CCNGG 3 cut(s) 62, 304, 422
BstUI CGCG 2 cut(s) 236, 268
BstV1I GCAGC 3 cut(s) 128, 597, 619
BstV2I GAAGAC 2 cut(s) 108, 441
BstXI CCANNNNNNTGG 1 cut(s) 70
BtgI CCRYGG 1 cut(s) 489
BtsCI GGATG 3 cut(s) 79, 211, 624
BtsIMutI CAGTG 3 cut(s) 160, 347, 559
CaiI CAGNNNCTG 1 cut(s) 610
CfoI GCGC 1 cut(s) 9
Cfr10I RCCGGY 1 cut(s) 355
CseI GACGC 1 cut(s) 531
Csp6I GTAC 1 cut(s) 584
CviAII CATG 1 cut(s) 490
CviJI RGCY 7 cut(s) 119, 215, 355, 413, 487, 610, 625
CviKI_1 RGCY 7 cut(s) 119, 215, 355, 413, 487, 610, 625
CviQI GTAC 1 cut(s) 584
DdeI CTNAG 1 cut(s) 120
DpnI GATC 3 cut(s) 318, 366, 574
DpnII GATC 3 cut(s) 316, 364, 572
DriI GACNNNNNGTC 1 cut(s) 474
Eam1104I CTCTTC 1 cut(s) 372
Eam1105I GACNNNNNGTC 1 cut(s) 474
EarI CTCTTC 1 cut(s) 372
EciI GGCGGA 1 cut(s) 551
Eco130I CCWWGG 1 cut(s) 489
Eco24I GRGCYC 1 cut(s) 489
Eco57I CTGAAG 1 cut(s) 371
EcoRI GAATTC 1 cut(s) 289
EcoRII CCWGG 2 cut(s) 62, 422
EcoT14I CCWWGG 1 cut(s) 489
EcoT38I GRGCYC 1 cut(s) 489
ErhI CCWWGG 1 cut(s) 489
FaeI CATG 1 cut(s) 493
FaiI YATR 5 cut(s) 228, 321, 410, 491, 648
FatI CATG 1 cut(s) 489
FauI CCCGC 2 cut(s) 241, 261
Fnu4HI GCNGC 3 cut(s) 117, 608, 611
FokI GGATG 3 cut(s) 86, 218, 631
FriOI GRGCYC 1 cut(s) 489
Fsp4HI GCNGC 3 cut(s) 117, 608, 611
GlaI GCGC 1 cut(s) 8
GluI GCNGC 3 cut(s) 117, 608, 611
HapII CCGG 2 cut(s) 305, 356
HgaI GACGC 1 cut(s) 531
HhaI GCGC 1 cut(s) 9
Hin1II CATG 1 cut(s) 493
Hin6I GCGC 1 cut(s) 7
HinP1I GCGC 1 cut(s) 7
HinfI GANTC 3 cut(s) 263, 301, 525
HpaII CCGG 2 cut(s) 305, 356
Hpy188I TCNGA 3 cut(s) 295, 376, 484
Hpy188III TCNNGA 2 cut(s) 453, 637
Hpy99I CGWCG 2 cut(s) 35, 38
HpyAV CCTTC 2 cut(s) 207, 563
HpyCH4III ACNGT 2 cut(s) 94, 447
HpyCH4V TGCA 6 cut(s) 89, 116, 166, 431, 473, 613
HpyF10VI GCNNNNNNNGC 1 cut(s) 607
HpyF3I CTNAG 1 cut(s) 120
Hsp92II CATG 1 cut(s) 493
HspAI GCGC 1 cut(s) 7
Kzo9I GATC 3 cut(s) 316, 364, 572
LmnI GCTCC 2 cut(s) 484, 622
Lsp1109I GCAGC 3 cut(s) 128, 597, 619
LweI GCATC 2 cut(s) 153, 604
MaeIII GTNAC 3 cut(s) 345, 460, 655
MalI GATC 3 cut(s) 318, 366, 574
MboI GATC 3 cut(s) 316, 364, 572
MboII GAAGA 4 cut(s) 113, 143, 389, 441
MhlI GDGCHC 2 cut(s) 225, 489
MluCI AATT 3 cut(s) 23, 289, 643
MmeI TCCRAC 2 cut(s) 354, 462
MnlI CCTC 3 cut(s) 355, 373, 644
MseI TTAA 1 cut(s) 311
MspA1I CMGCKG 1 cut(s) 610
MspI CCGG 2 cut(s) 305, 356
MspR9I CCNGG 3 cut(s) 64, 306, 424
MvaI CCWGG 2 cut(s) 64, 424
MvnI CGCG 2 cut(s) 236, 268
MwoI GCNNNNNNNGC 1 cut(s) 607
NciI CCSGG 1 cut(s) 306
NcoI CCATGG 1 cut(s) 489
NdeII GATC 3 cut(s) 316, 364, 572
NlaIII CATG 1 cut(s) 493
NlaIV GGNNCC 3 cut(s) 189, 486, 624
NmuCI GTSAC 3 cut(s) 345, 460, 655
PasI CCCWGGG 1 cut(s) 423
PfeI GAWTC 3 cut(s) 263, 301, 525
PfoI TCCNGGA 1 cut(s) 62
PkrI GCNGC 3 cut(s) 118, 609, 612
Psp6I CCWGG 2 cut(s) 62, 422
PspGI CCWGG 2 cut(s) 62, 422
PspN4I GGNNCC 3 cut(s) 189, 486, 624
PstNI CAGNNNCTG 1 cut(s) 610
PvuII CAGCTG 1 cut(s) 610
RsaI GTAC 1 cut(s) 585
RsaNI GTAC 1 cut(s) 584
SaqAI TTAA 1 cut(s) 311
SatI GCNGC 3 cut(s) 117, 608, 611
Sau3AI GATC 3 cut(s) 316, 364, 572
ScrFI CCNGG 3 cut(s) 64, 306, 424
SduI GDGCHC 2 cut(s) 225, 489
SetI ASST 6 cut(s) 121, 126, 193, 217, 262, 612
SfaNI GCATC 2 cut(s) 153, 604
Sse9I AATT 3 cut(s) 23, 289, 643
SsiI CCGC 3 cut(s) 234, 268, 536
StyD4I CCNGG 3 cut(s) 62, 304, 422
StyI CCWWGG 1 cut(s) 489
TaaI ACNGT 2 cut(s) 94, 447
TaqI TCGA 1 cut(s) 575
TasI AATT 3 cut(s) 23, 289, 643
TfiI GAWTC 3 cut(s) 263, 301, 525
Tru1I TTAA 1 cut(s) 311
Tru9I TTAA 1 cut(s) 311
TscAI CASTG 3 cut(s) 160, 354, 559
TseFI GTSAC 3 cut(s) 345, 460, 655
TseI GCWGC 3 cut(s) 116, 607, 610
Tsp45I GTSAC 3 cut(s) 345, 460, 655
TspDTI ATGAA 2 cut(s) 17, 114
TspGWI ACGGA 1 cut(s) 200
TspRI CASTG 3 cut(s) 160, 354, 559
XapI RAATTY 1 cut(s) 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.