pycom08g11480

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0009462
Physical Location & Seq
Forward (+)
9689979 .. 9704667
14689 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4992 bp
ATGGATGATCCGCTTCCTATGACTTTCGATAGGTCTAACGGAGATGGGACGTCCAGTAAAATTTTCCCATTTACTGAAAATGTTGCAGGAACTAAAGCCATAAGTGGTAATGTAAACGATGAGGATTTTGGAGTCGATGTTGTAAATAAGCATACTGAGATATCAGAGAGGCAAAGAGGAAAGCCATTAGCCTTTCTCTCTGGACCCATTCAAAAGCTGACGTCTAGTGTGGGCCCCAGAGTTGAAGATATTGTTGCAGAACTTGTCGACGGGGTCGGTGTCGGGCAGTCTGAAAGCATTGAGAAGATGCTTCCAGTTACACTAGATTCTGTTCATTTCAAAGGAGGAACATTGATGTTACTTGCATATGGTGACAGGGAGCCTCGAGAGATGGAGAATGTCGATGGACATGTGAAGTTTCAAAATCACTATGGCCAAGTTCACGTACAAGTTAGTGGAAACTGTAAGGTGTGGAGGTCTGATAACATATCTGAAGATGGCGGCTGGTTGTCTACAGATGTTTTTGTTGACATTGTTGAACAGAAATGGCATGCCAACTTAAAAGTTGCCAACTTGTTTGTTCCGCTTTTTGAAAGGATCTTAGAAATTCCAATAATTTGGTCCAGAGGAAGAGCCACTGGTGAGGTTCACTTGTGCATGTCAAGTGGAGAAACATTTCCTAATCTTCATGGACAGCTTGATGTGACAGGGTTGGCTTTTCAAACAATCGGCGCGCCTTCATCATTTTCTGATATTTCAGCAAGCTTATGTTTCCGTGGCCAACGGATATTCTTACACAATGCAAGTGGCTGGTTTGGGGATGTTCCCTTGGAAGCTTCTGGTGATTTTGGCATTCATCCTGAGGAAGGAGAGTTTCATCTTATGTGTCAGGTTTCTTGCGTGGAAGTAAATTCCCTGATGAGAACTTTCAAGATGAAACCTCTCTTGTTTCCGCTGGCTGGTTCAGTGACTGCTGTGTTTAACTGTCAAGGTCCACTGGATGCACCTATATTTGTGGGAAGTGGAATGGTCTCTAGAAGGATATCTCATTCAGTTACTGATTTTCCTCCGTCCTCTGCATCTGAAGCAGTTTTGAAAAGTAAAGAAGCCGGTGCAGTAGCAGCATTCGATCGTGTACCATTTTCATGTGTATCGGCTAATTTCACTTTTAACACTGATAGCTGTGTTGCTGATTTGTATGGAATAAGAGCTAGTCTTGTAGATGGCGGTGAAATTCGAGGGGCAGGGAATGCATGGATTTGCCCAGAGGGTGAAGTTGATGATACATCAATGGATGTGAATTTCTCTGGAAGTATGTGTTTTGATAAAATTCTGCATCGATATGTACCTGGGTATCTTCAACTAATGCCACTCAAATTAGGGGTTTTAAATGGAGAGACAAAACTTTCTGGTTCACTTTTGAGACCGAGGTTTGACATTAAATGGACAGCTCCAAATGCTGAAGGGTCCTTCAGCGATGCTAGAGGAGATATCATAATTTCACACGATTCCATTACTGTTAATTCCTCATCTGCTGCTTTTGATTTGTCATCGAAAGTGCAAACATCTTACACTGACAAAATCTGCTTGCACAGAAAAGATGGTTATGGAAAGAGTGCCATGCCATTTGTTGTTGAGGGAATTGACTTGGATTTGCGTATGCGTAGTTTTGAGTTTTTTAACGTGGTATCTCCTTATACCTTCGATTCTCCGAAACCAATGCATTTAAAAGCAACAGGCAAGATCAAGTTTCAAGGCAAGGTTGTAAAGCCTTGCAGTTTTGAAAATGGACAAGATTTTGGTCTAGATAGAAATAAGCTCCCTGTGGCAATGACATATAAAGAAAAGACAGACAGTCTTGTTGGAGAGGTTTCGATTTCTGGTCTTAGATTGAATCAGTTGATGTTGGCGCCTCAGTTGGCTGGATCATTGAGCTTGTCACCTGAGTATATCAAGTTGGATGCCACTGGTAGGCCAGATGAAAGTCTTGTAGTGGAGTTTGTTGGGCCGTTGAAGCCTAATAATGAAGATAATTCCCCAAGTGGACAGTTGTTATCTTTTTTGCTTCAAAAAGGGCAACTAAAAGCAAACATTTGCTTCCAGCCATTTCATTCTGCTAGTTTGGAGATACGTCAGTTACCACTGGATGAGTTGGAGTTGGCCTCACTTCGTGGAACAATACAGAAGGCAGAAATTCAACTTAATTTTCAGAAACGAAGAGGTCATGGACTGCTATCTGTACTTCGACCGAAATTCAGTGGAGTGCTAGGTGAAGCCCTTGATGTCGCTGCTCGATGGAGTGGAGATGTTATCACTGTTGAGAAAACGGTCCTGGAGCAAAGCAATAGCCGTTATGAGCTTCAGGGTGAATATGTGTTGCCAGGCTCACGAGACCGCAACCCTGCTGGAAAGGAAAAGGGTGGCTTGTTGGAAAGGGCAATGGCTGGGCATCTGGGAAGTGTCATATCTTCTATGGGAAGGTGGAGAATGAGACTGGAAGTTCCCAGAGCTGAGGTTGCTGAGATGCTTCCCCTAGCCAGACTTGTTTCTCGAAGTACAGATCCTGCTGTTCATTCTAGGTCAAAGGATCTCTTTATTCAGAGTTTGCAGTCTGTGGGATTATATACTGAAAGTCTTAAAGAACTACTCGAGGTTATACGGGGACATTATACTCCATCAAATGAAGTTATTCTAGAAGACTTAAATCTTCCAGGTTTAACAGAGCTTAGAGGTAGATGGCATGGCTCTCTTGATGCTAGTGGTGGTGGCAATGGGGATACCATGGCAGAATTTGATTTTCATGGGGAGGATTGGGAGTGGGGTACATACAAAACTCAGCGAGTTCTGGCAGTTGGTGCATACAGCAATGATGATGGTTTGCGCCTGGAAAAGATGTTTATTCAGAAAGATAATGCTACAATTCATGCAGATGGGACTTTGTTAGGGCCTAAAACGAATCTTCACTTTGCTGTTCTAAATTTTCCTGTTAGTCTAGTTCCGACGGTGATTCAGGTGATTGAATCTTCTGCTACTGATGTGGTTCAATCTTTAAGGAAGTTTTTAGCACCAATTAGGGGTATATTGCACATGGAAGGCGATCTCAGAGGGAATCTTGCAAAACCAGAATGTGATGTGCAAGTAAGGCTTCTGGATGGTGCCATTGGGGGGATCGATCTTGGACGAGCTGAAGTTGTTGCTTCTCTAACTTCAACCAGTCGTTTTCTGTTCAACGCAAAATTTGAGCCAATCATCCAAATTGGGCATGTTCACATTCAAGGAAGTGTTCCTGTTACTTTTGTTCAGAATAACATGTTAGAGGAAGAAGATTTAGAGAAAGATAGAGGTCGAGCAAGTTGGGAAGGCTGGGTGAAAGAAAGAGGTAGGGCATCTGTAGATGATGCCACTGAAAAGAAACTTTCAAGAGAGCGAAATGAAGAAGGTTGGGATACTCGATTAGCAGAAAGCCTCAAAGGTTTAAACTGGAACCTACTGGATGTAGGGGAAGTCAGAATAGATGCTGATGTTAAAGATGGGGGCATGATGCTGTTAACTGCTTTATCGCCTTATGCTAAATGGCTTCAGGGCAATGCTGACGTCATGCTTCAGGTCAGAGGCACAGTTGAACAACCGGTGCTGGATGGGTATGCATCATTCCACAGGGCATCTATATCATCACCAGTTCTCTGGAAACCACTGTCAAACTTCGGTGGCACAGTTCATGTGAAATCAAATAGGTTGTGCATCACTTCGTTGGAGAGTAGGGTAAGCAGAAGGGGAAAACTATTCATGAAGGGAAACCTGCCCCTTAGAACAAGTGAAGCATCCCTTGGAGATAAAATTGACTTGAAATGTGAAGTTTTGGAAGTGCGGGCAAAGAATATTCTGAGTGCCCAGGTTGACACTCAGATGCAGATAACTGGTTCTATATTGCAACCAAACATCTCTGGAAATATTAAATTGAGCCATGGTGAAGCTTATCTACCACATGATAAGGGTAGTGGGGCTACTTCTAATAGATTGGTGTCAAATGAGTCTAGGCTTCTAGCTACTGGTGTCGATCGAGCAGTTGCCTCGAGGTATGTTTCACGGTTTTTCAGTTCCCAACCTGCTGCTTCAAGGACAAAATTCTCTCAACCCTCAGTTGAACCAGCTGAAAAGGAGATGGAGCAAGTGAACATTAAACCGAACGTAGATATTCAGCTTAGTGATTTAAAGCTTGCTCTAGGACCAGAATTGAGAGTAGTTTATCCTTTAATTCTCAATTTCGCAGTCAGTGGAGAGCTTGAGTTAAATGGCCCGGCTCATCCCAAATTGATACAACCTAGAGGAGTCTTAACTTTTGAGAATGGTGACGTGAATCTTGTGGCAACTCAGGTGACTTATTGGCTCAAGCAAGAGCATCTGAATATTGCAAAATTCGAGCCTGAAATTGGACTAGATCCTATGCTAGATTTAGTTCTGGTAGGTTCAGAGTGGCAATTCAGGATTCAAAGTCGAGCCAGCAATTGGCAGGACAAACTGGTGGTGACATCCACTCGCTCTGTGGAACAGGATGCCCTCTCACCGACCGAGGCTGCTAGAGTCTTTGAAAGTCAACTGGCGGAGTCCATATTGGAAAACGATGGGCAGCTTGCATTCCAAAAGCTTGCCACTACGACACTGGAAAAACTAATGCCAAGAATAGAAGGGAAGGGTGAGTTTGGGCAGGCAAGGTGGAGGCTAGTGTATGCACCGCAGATCCCTAGTTTGCTTTCTGTTGATCCTACGGTGGATCCTCTCAAATCCCTGGCCAGTAATATTTCCTTTGGTACAGAAGTTGAAGTCCAACTCGGGAAACGTCTTCAGGCCTCGATTGTTAGGCAAATGAAGGACTCAGAGATGGCAATGCAATGGACGTTGATCTACCAGCTTACGAGCCGCTTGAGGGTTCTCCTGCAGTCTGCTCCGTCGAAGCGCCTCCTTTTCGAATATTCTGCCACCTCGCAGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TamB

Protein Analysis

1664

Amino Acids

182.96

Weight (kDa)

5.71

Isoelectric Point (pI)

37.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TamB PF04357 1251 - 1647 7.7e-17 TamB C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 53, 224, 3597
AbsI CCTCGAGG 1 cut(s) 4072
Acc36I ACCTGC 2 cut(s) 3807, 4114
AccB1I GGYRCC 2 cut(s) 1909, 3155
AccB7I CCANNNNNTGG 1 cut(s) 4506
AccI GTMKAC 2 cut(s) 267, 512
AccII CGCG 1 cut(s) 734
AcoI YGGCCR 3 cut(s) 433, 778, 4788
AcuI CTGAAG 9 cut(s) 513, 1104, 1456, 1482, 2345, 3207, 3563, 3587, 4826
AcyI GRCGYC 4 cut(s) 50, 221, 1910, 3594
AdeI CACNNNGTG 1 cut(s) 2171
AfaI GTAC 7 cut(s) 447, 1137, 1347, 2241, 2557, 2824, 4810
AfiI CCNNNNNNNGG 8 cut(s) 866, 959, 1971, 3074, 3165, 4430, 4506, 4924
AflIII ACRYGT 2 cut(s) 409, 3309
AgeI ACCGGT 1 cut(s) 3628
AhdI GACNNNNNGTC 1 cut(s) 1854
AjiI CACGTC 1 cut(s) 4354
AjnI CCWGG 7 cut(s) 1348, 2331, 2380, 2710, 2883, 3891, 4785
AjuI GAANNNNNNNTTGG 2 cut(s) 3810, 3842
Alw26I GTCTC 5 cut(s) 1036, 1391, 1417, 2385, 2485
AlwNI CAGNNNCTG 3 cut(s) 971, 1058, 2564
Ama87I CYCGRG 4 cut(s) 384, 2648, 4072, 4829
ApaI GGGCCC 1 cut(s) 236
ApeKI GCWGC 6 cut(s) 1121, 1535, 2288, 4109, 4574, 4627
AscI GGCGCGCC 1 cut(s) 732
AsiGI ACCGGT 1 cut(s) 3628
Asp700I GAANNNNTTC 2 cut(s) 675, 2688
AspLEI GCGC 5 cut(s) 734, 736, 1912, 2883, 4956
AsuC2I CCSGG 1 cut(s) 4298
AsuII TTCGAA 1 cut(s) 4965
AvaI CYCGRG 4 cut(s) 384, 2648, 4072, 4829
AvaII GGWCC 6 cut(s) 203, 621, 992, 1467, 2329, 4226
AxyI CCTNAGG 1 cut(s) 861
BaeGI GKGCMC 2 cut(s) 236, 3892
BaeI ACNNNNGTAYC 6 cut(s) 1337, 1370, 2120, 2153, 4800, 4833
BalI TGGCCA 3 cut(s) 435, 780, 4790
BamHI GGATCC 1 cut(s) 4771
BanI GGYRCC 2 cut(s) 1909, 3155
BanII GRGCYC 1 cut(s) 236
BarI GAAGNNNNNNTAC 2 cut(s) 3963, 3995
BauI CACGAG 1 cut(s) 2388
BbsI GAAGAC 2 cut(s) 2703, 4832
BbvCI CCTCAGC 1 cut(s) 2511
BbvI GCAGC 6 cut(s) 1133, 1522, 2275, 4096, 4561, 4639
BceAI ACGGC 2 cut(s) 1993, 2334
BcgI CGANNNNNNTGC 6 cut(s) 1702, 1736, 4053, 4087, 4955, 4989
BciT130I CCWGG 7 cut(s) 1350, 2333, 2382, 2712, 2885, 3893, 4787
BciVI GTATCC 2 cut(s) 2770, 3439
BcnI CCSGG 1 cut(s) 4298
BcoDI GTCTC 5 cut(s) 1036, 1391, 1417, 2385, 2485
BfmI CTRYAG 3 cut(s) 513, 3390, 4934
BfoI RGCGCY 2 cut(s) 1913, 4957
BfuAI ACCTGC 2 cut(s) 3807, 4114
BfuI GTATCC 2 cut(s) 2770, 3439
BisI GCNGC 8 cut(s) 502, 1122, 1536, 2289, 4110, 4575, 4628, 4918
BlsI GCNGC 8 cut(s) 503, 1123, 1537, 2290, 4111, 4576, 4629, 4919
Bme1390I CCNGG 8 cut(s) 1350, 2333, 2382, 2712, 2885, 3893, 4298, 4787
Bme18I GGWCC 6 cut(s) 203, 621, 992, 1467, 2329, 4226
BmeRI GACNNNNNGTC 1 cut(s) 1854
BmeT110I CYCGRG 4 cut(s) 384, 2648, 4072, 4829
BmgBI CACGTC 1 cut(s) 4354
BmiI GGNNCC 9 cut(s) 205, 234, 235, 381, 1468, 1911, 3157, 3485, 4773
BmrFI CCNGG 8 cut(s) 1350, 2333, 2382, 2712, 2885, 3893, 4298, 4787
BpiI GAAGAC 2 cut(s) 2703, 4832
BplI GAGNNNNNCTC 2 cut(s) 2147, 2179
BpmI CTGGAG 1 cut(s) 2354
Bpu10I CCTNAGC 1 cut(s) 2511
Bpu14I TTCGAA 1 cut(s) 4965
BpuEI CTTGAG 3 cut(s) 4304, 4373, 4942
BpuMI CCSGG 1 cut(s) 4298
Bsa29I ATCGAT 2 cut(s) 1339, 3171
BsaAI YACGTR 1 cut(s) 445
BsaHI GRCGYC 4 cut(s) 50, 221, 1910, 3594
BsaI GGTCTC 3 cut(s) 1036, 1417, 2385
BsaWI WCCGGW 1 cut(s) 3628
BsaXI ACNNNNNCTCC 2 cut(s) 123, 153
Bsc4I CCNNNNNNNGG 8 cut(s) 866, 959, 1971, 3074, 3165, 4430, 4506, 4924
Bse118I RCCGGY 2 cut(s) 1109, 3628
Bse21I CCTNAGG 1 cut(s) 861
Bse3DI GCAATG 7 cut(s) 1836, 2445, 2776, 2872, 3592, 4890, 4895
BseBI CCWGG 7 cut(s) 1350, 2333, 2382, 2712, 2885, 3893, 4787
BseCI ATCGAT 2 cut(s) 1339, 3171
BseLI CCNNNNNNNGG 8 cut(s) 866, 959, 1971, 3074, 3165, 4430, 4506, 4924
BseMI GCAATG 7 cut(s) 1836, 2445, 2776, 2872, 3592, 4890, 4895
BsePI GCGCGC 1 cut(s) 732
BseRI GAGGAG 2 cut(s) 1500, 4341
BseSI GKGCMC 2 cut(s) 236, 3892
BseXI GCAGC 6 cut(s) 1133, 1522, 2275, 4096, 4561, 4639
BseYI CCCAGC 2 cut(s) 2444, 3363
BsgI GTGCAG 1 cut(s) 1134
Bsh1236I CGCG 1 cut(s) 734
Bsh1285I CGRYCG 4 cut(s) 1132, 2249, 4060, 4569
BshNI GGYRCC 2 cut(s) 1909, 3155
BshTI ACCGGT 1 cut(s) 3628
BshVI ATCGAT 2 cut(s) 1339, 3171
BsiEI CGRYCG 4 cut(s) 1132, 2249, 4060, 4569
BsiHKCI CYCGRG 4 cut(s) 384, 2648, 4072, 4829
BsiSI CCGG 3 cut(s) 1110, 3629, 4298
BslFI GGGAC 3 cut(s) 61, 2676, 2947
BslI CCNNNNNNNGG 8 cut(s) 866, 959, 1971, 3074, 3165, 4430, 4506, 4924
BsmAI GTCTC 5 cut(s) 1036, 1391, 1417, 2385, 2485
BsmFI GGGAC 3 cut(s) 61, 2676, 2947
BsmI GAATGC 4 cut(s) 852, 1124, 1255, 4634
Bso31I GGTCTC 3 cut(s) 1036, 1417, 2385
BsoBI CYCGRG 4 cut(s) 384, 2648, 4072, 4829
Bsp119I TTCGAA 1 cut(s) 4965
Bsp120I GGGCCC 1 cut(s) 232
Bsp1286I GDGCHC 2 cut(s) 236, 3892
Bsp19I CCATGG 2 cut(s) 2781, 3964
BspDI ATCGAT 2 cut(s) 1339, 3171
BspFNI CGCG 1 cut(s) 734
BspHI TCATGA 1 cut(s) 3786
BspLI GGNNCC 9 cut(s) 205, 234, 235, 381, 1468, 1911, 3157, 3485, 4773
BspMAI CTGCAG 1 cut(s) 4938
BspMI ACCTGC 2 cut(s) 3807, 4114
BspQI GCTCTTC 1 cut(s) 625
BspT104I TTCGAA 1 cut(s) 4965
BspT107I GGYRCC 2 cut(s) 1909, 3155
BspTNI GGTCTC 3 cut(s) 1036, 1417, 2385
BsrDI GCAATG 7 cut(s) 1836, 2445, 2776, 2872, 3592, 4890, 4895
BsrFI RCCGGY 2 cut(s) 1109, 3628
BssAI RCCGGY 2 cut(s) 1109, 3628
BssHII GCGCGC 1 cut(s) 732
BssNI GRCGYC 4 cut(s) 50, 221, 1910, 3594
BssSI CACGAG 1 cut(s) 2388
BssT1I CCWWGG 4 cut(s) 828, 2781, 3826, 3964
Bst2BI CACGAG 1 cut(s) 2388
Bst2UI CCWGG 7 cut(s) 1350, 2333, 2382, 2712, 2885, 3893, 4787
Bst6I CTCTTC 2 cut(s) 625, 2212
BstACI GRCGYC 4 cut(s) 50, 221, 1910, 3594
BstAPI GCANNNNNTGC 2 cut(s) 1250, 2855
BstBAI YACGTR 1 cut(s) 445
BstBI TTCGAA 1 cut(s) 4965
BstDSI CCRYGG 3 cut(s) 775, 2781, 3964
BstENI CCTNNNNNAGG 1 cut(s) 864
BstFNI CGCG 1 cut(s) 734
BstH2I RGCGCY 2 cut(s) 1913, 4957
BstHHI GCGC 5 cut(s) 734, 736, 1912, 2883, 4956
BstMAI GTCTC 5 cut(s) 1036, 1391, 1417, 2385, 2485
BstMCI CGRYCG 4 cut(s) 1132, 2249, 4060, 4569
BstMWI GCNNNNNNNGC 8 cut(s) 1085, 1121, 1250, 1457, 2014, 2516, 2855, 3142
BstNI CCWGG 7 cut(s) 1350, 2333, 2382, 2712, 2885, 3893, 4787
BstNSI RCATGY 5 cut(s) 413, 554, 661, 3266, 3313
BstSCI CCNGG 8 cut(s) 1348, 2331, 2380, 2710, 2883, 3891, 4296, 4785
BstSFI CTRYAG 3 cut(s) 513, 3390, 4934
BstSLI GKGCMC 2 cut(s) 236, 3892
BstUI CGCG 1 cut(s) 734
BstV1I GCAGC 6 cut(s) 1133, 1522, 2275, 4096, 4561, 4639
BstV2I GAAGAC 2 cut(s) 2703, 4832
BstX2I RGATCY 6 cut(s) 597, 2560, 2587, 4438, 4737, 4771
BstXI CCANNNNNNTGG 2 cut(s) 618, 3684
BstYI RGATCY 6 cut(s) 597, 2560, 2587, 4438, 4737, 4771
Bsu15I ATCGAT 2 cut(s) 1339, 3171
Bsu36I CCTNAGG 1 cut(s) 861
BsuI GTATCC 2 cut(s) 2770, 3439
BsuTUI ATCGAT 2 cut(s) 1339, 3171
BtgI CCRYGG 3 cut(s) 775, 2781, 3964
BtgZI GCGATG 1 cut(s) 1491
BtrI CACGTC 1 cut(s) 4354
BveI ACCTGC 2 cut(s) 3807, 4114
CaiI CAGNNNCTG 3 cut(s) 971, 1058, 2564
CciI TCATGA 1 cut(s) 3786
CfoI GCGC 5 cut(s) 734, 736, 1912, 2883, 4956
Cfr10I RCCGGY 2 cut(s) 1109, 3628
ClaI ATCGAT 2 cut(s) 1339, 3171
Csp6I GTAC 7 cut(s) 446, 1136, 1346, 2240, 2556, 2823, 4809
CspAI ACCGGT 1 cut(s) 3628
CspCI CAANNNNNGTGG 2 cut(s) 787, 822
CviQI GTAC 7 cut(s) 446, 1136, 1346, 2240, 2556, 2823, 4809
DinI GGCGCC 1 cut(s) 1911
DraI TTTAAA 4 cut(s) 1389, 1728, 3477, 4212
DraIII CACNNNGTG 1 cut(s) 2171
DriI GACNNNNNGTC 1 cut(s) 1854
EaeI YGGCCR 3 cut(s) 433, 778, 4788
Eam1104I CTCTTC 2 cut(s) 625, 2212
Eam1105I GACNNNNNGTC 1 cut(s) 1854
EarI CTCTTC 2 cut(s) 625, 2212
EciI GGCGGA 1 cut(s) 4616
Eco130I CCWWGG 4 cut(s) 828, 2781, 3826, 3964
Eco147I AGGCCT 1 cut(s) 4847
Eco24I GRGCYC 1 cut(s) 236
Eco31I GGTCTC 3 cut(s) 1036, 1417, 2385
Eco32I GATATC 3 cut(s) 162, 1044, 1492
Eco47I GGWCC 6 cut(s) 203, 621, 992, 1467, 2329, 4226
Eco57I CTGAAG 9 cut(s) 513, 1104, 1456, 1482, 2345, 3207, 3563, 3587, 4826
Eco81I CCTNAGG 1 cut(s) 861
Eco88I CYCGRG 4 cut(s) 384, 2648, 4072, 4829
EcoNI CCTNNNNNAGG 1 cut(s) 864
EcoO109I RGGNCCY 3 cut(s) 233, 1467, 2945
EcoRII CCWGG 7 cut(s) 1348, 2331, 2380, 2710, 2883, 3891, 4785
EcoRV GATATC 3 cut(s) 162, 1044, 1492
EcoT14I CCWWGG 4 cut(s) 828, 2781, 3826, 3964
EcoT22I ATGCAT 3 cut(s) 1255, 1725, 3649
EcoT38I GRGCYC 1 cut(s) 236
EgeI GGCGCC 1 cut(s) 1911
EheI GGCGCC 1 cut(s) 1911
ErhI CCWWGG 4 cut(s) 828, 2781, 3826, 3964
FalI AAGNNNNNCTT 8 cut(s) 2408, 2440, 2576, 2608, 3129, 3161, 3810, 3842
FaqI GGGAC 3 cut(s) 61, 2676, 2947
FauI CCCGC 1 cut(s) 3861
FauNDI CATATG 1 cut(s) 367
FblI GTMKAC 2 cut(s) 267, 512
Fnu4HI GCNGC 8 cut(s) 502, 1122, 1536, 2289, 4110, 4575, 4628, 4918
FriOI GRGCYC 1 cut(s) 236
Fsp4HI GCNGC 8 cut(s) 502, 1122, 1536, 2289, 4110, 4575, 4628, 4918
GlaI GCGC 5 cut(s) 733, 735, 1911, 2882, 4955
GluI GCNGC 8 cut(s) 502, 1122, 1536, 2289, 4110, 4575, 4628, 4918
GsaI CCCAGC 2 cut(s) 2448, 3367
GsuI CTGGAG 1 cut(s) 2354
HaeII RGCGCY 2 cut(s) 1913, 4957
HapII CCGG 3 cut(s) 1110, 3629, 4298
HhaI GCGC 5 cut(s) 734, 736, 1912, 2883, 4956
Hin1I GRCGYC 4 cut(s) 50, 221, 1910, 3594
Hin6I GCGC 5 cut(s) 732, 734, 1910, 2881, 4954
HinP1I GCGC 5 cut(s) 732, 734, 1910, 2881, 4954
HincII GTYRAC 5 cut(s) 268, 529, 3549, 3898, 4595
HindII GTYRAC 5 cut(s) 268, 529, 3549, 3898, 4595
HindIII AAGCTT 5 cut(s) 763, 834, 3972, 4214, 4643
HpaI GTTAAC 1 cut(s) 3549
HpaII CCGG 3 cut(s) 1110, 3629, 4298
Hpy99I CGWCG 3 cut(s) 272, 3004, 4951
HpyF10VI GCNNNNNNNGC 8 cut(s) 1085, 1121, 1250, 1457, 2014, 2516, 2855, 3142
Hsp92I GRCGYC 4 cut(s) 50, 221, 1910, 3594
HspAI GCGC 5 cut(s) 732, 734, 1910, 2881, 4954
KasI GGCGCC 1 cut(s) 1909
KspAI GTTAAC 1 cut(s) 3549
LguI GCTCTTC 1 cut(s) 625
LmnI GCTCC 6 cut(s) 379, 1456, 1824, 2335, 4165, 4948
Lsp1109I GCAGC 6 cut(s) 1133, 1522, 2275, 4096, 4561, 4639
MfeI CAATTG 1 cut(s) 4504
MflI RGATCY 6 cut(s) 597, 2560, 2587, 4438, 4737, 4771
MhlI GDGCHC 2 cut(s) 236, 3892
MlsI TGGCCA 3 cut(s) 435, 780, 4790
MluNI TGGCCA 3 cut(s) 435, 780, 4790
Mly113I GGCGCC 1 cut(s) 1910
MlyI GAGTC 6 cut(s) 141, 4040, 4338, 4590, 4613, 4865
MmeI TCCRAC 7 cut(s) 1843, 1938, 2133, 2409, 3023, 3732, 4849
Mox20I TGGCCA 3 cut(s) 435, 780, 4790
Mph1103I ATGCAT 3 cut(s) 1255, 1725, 3649
MroXI GAANNNNTTC 2 cut(s) 675, 2688
MscI TGGCCA 3 cut(s) 435, 780, 4790
MslI CAYNNNNRTG 4 cut(s) 1144, 1341, 2928, 3905
Msp20I TGGCCA 3 cut(s) 435, 780, 4790
MspA1I CMGCKG 2 cut(s) 955, 4151
MspI CCGG 3 cut(s) 1110, 3629, 4298
MspR9I CCNGG 8 cut(s) 1350, 2333, 2382, 2712, 2885, 3893, 4298, 4787
MssI GTTTAAAC 1 cut(s) 3477
MunI CAATTG 1 cut(s) 4504
Mva1269I GAATGC 4 cut(s) 852, 1124, 1255, 4634
MvaI CCWGG 7 cut(s) 1350, 2333, 2382, 2712, 2885, 3893, 4787
MvnI CGCG 1 cut(s) 734
MwoI GCNNNNNNNGC 8 cut(s) 1085, 1121, 1250, 1457, 2014, 2516, 2855, 3142
NarI GGCGCC 1 cut(s) 1910
NciI CCSGG 1 cut(s) 4298
NcoI CCATGG 2 cut(s) 2781, 3964
NdeI CATATG 1 cut(s) 367
NlaIV GGNNCC 9 cut(s) 205, 234, 235, 381, 1468, 1911, 3157, 3485, 4773
NmuCI GTSAC 7 cut(s) 371, 703, 967, 1938, 4349, 4375, 4525
NsiI ATGCAT 3 cut(s) 1255, 1725, 3649
NspI RCATGY 5 cut(s) 413, 554, 661, 3266, 3313
NspV TTCGAA 1 cut(s) 4965
PaeI GCATGC 1 cut(s) 554
PaeR7I CTCGAG 3 cut(s) 384, 2648, 4072
PagI TCATGA 1 cut(s) 3786
PalAI GGCGCGCC 1 cut(s) 732
PauI GCGCGC 1 cut(s) 732
PceI AGGCCT 1 cut(s) 4847
PciI ACATGT 2 cut(s) 409, 3309
PciSI GCTCTTC 1 cut(s) 625
PctI GAATGC 4 cut(s) 852, 1124, 1255, 4634
PdmI GAANNNNTTC 2 cut(s) 675, 2688
PflFI GACNNNGTC 1 cut(s) 272
PflMI CCANNNNNTGG 1 cut(s) 4506
PfoI TCCNGGA 1 cut(s) 2331
PinAI ACCGGT 1 cut(s) 3628
PkrI GCNGC 8 cut(s) 503, 1123, 1537, 2290, 4111, 4576, 4629, 4919
Ple19I CGATCG 2 cut(s) 1132, 4060
PleI GAGTC 6 cut(s) 140, 4039, 4337, 4589, 4612, 4865
PluTI GGCGCC 1 cut(s) 1913
PmeI GTTTAAAC 1 cut(s) 3477
PpsI GAGTC 6 cut(s) 140, 4039, 4337, 4589, 4612, 4865
Ppu21I YACGTR 1 cut(s) 445
PpuMI RGGWCCY 1 cut(s) 1467
PscI ACATGT 2 cut(s) 409, 3309
Psp5II RGGWCCY 1 cut(s) 1467
Psp6I CCWGG 7 cut(s) 1348, 2331, 2380, 2710, 2883, 3891, 4785
PspFI CCCAGC 2 cut(s) 2444, 3363
PspGI CCWGG 7 cut(s) 1348, 2331, 2380, 2710, 2883, 3891, 4785
PspN4I GGNNCC 9 cut(s) 205, 234, 235, 381, 1468, 1911, 3157, 3485, 4773
PspOMI GGGCCC 1 cut(s) 232
PspPPI RGGWCCY 1 cut(s) 1467
PspXI VCTCGAGB 2 cut(s) 2648, 4072
PstI CTGCAG 1 cut(s) 4938
PstNI CAGNNNCTG 3 cut(s) 971, 1058, 2564
PsuI RGATCY 6 cut(s) 597, 2560, 2587, 4438, 4737, 4771
PsyI GACNNNGTC 1 cut(s) 272
PteI GCGCGC 1 cut(s) 732
PvuI CGATCG 2 cut(s) 1132, 4060
PvuII CAGCTG 1 cut(s) 4151
RsaI GTAC 7 cut(s) 447, 1137, 1347, 2241, 2557, 2824, 4810
RsaNI GTAC 7 cut(s) 446, 1136, 1346, 2240, 2556, 2823, 4809
RseI CAYNNNNRTG 4 cut(s) 1144, 1341, 2928, 3905
SalI GTCGAC 1 cut(s) 266
SapI GCTCTTC 1 cut(s) 625
SatI GCNGC 8 cut(s) 502, 1122, 1536, 2289, 4110, 4575, 4628, 4918
SchI GAGTC 6 cut(s) 141, 4040, 4338, 4590, 4613, 4865
ScrFI CCNGG 8 cut(s) 1350, 2333, 2382, 2712, 2885, 3893, 4298, 4787
SduI GDGCHC 2 cut(s) 236, 3892
SfcI CTRYAG 3 cut(s) 513, 3390, 4934
SfoI GGCGCC 1 cut(s) 1911
Sfr274I CTCGAG 3 cut(s) 384, 2648, 4072
SfuI TTCGAA 1 cut(s) 4965
SgsI GGCGCGCC 1 cut(s) 732
SinI GGWCC 6 cut(s) 203, 621, 992, 1467, 2329, 4226
SlaI CTCGAG 3 cut(s) 384, 2648, 4072
SmiMI CAYNNNNRTG 4 cut(s) 1144, 1341, 2928, 3905
SmlI CTYRAG 6 cut(s) 384, 2648, 4072, 4283, 4388, 4921
SmoI CTYRAG 6 cut(s) 384, 2648, 4072, 4283, 4388, 4921
SphI GCATGC 1 cut(s) 554
SseBI AGGCCT 1 cut(s) 4847
SspDI GGCGCC 1 cut(s) 1909
SspI AATATT 5 cut(s) 3880, 3952, 4408, 4798, 4970
StuI AGGCCT 1 cut(s) 4847
StyD4I CCNGG 8 cut(s) 1348, 2331, 2380, 2710, 2883, 3891, 4296, 4785
StyI CCWWGG 4 cut(s) 828, 2781, 3826, 3964
TaqII GACCGA 3 cut(s) 1441, 2263, 4583
TatI WGTACW 2 cut(s) 2239, 2555
TauI GCSGC 2 cut(s) 504, 4920
TseFI GTSAC 7 cut(s) 371, 703, 967, 1938, 4349, 4375, 4525
TseI GCWGC 6 cut(s) 1121, 1535, 2288, 4109, 4574, 4627
Tsp45I GTSAC 7 cut(s) 371, 703, 967, 1938, 4349, 4375, 4525
TspGWI ACGGA 5 cut(s) 54, 764, 799, 1059, 4935
Tth111I GACNNNGTC 1 cut(s) 272
Van91I CCANNNNNTGG 1 cut(s) 4506
VpaK11BI GGWCC 6 cut(s) 203, 621, 992, 1467, 2329, 4226
XagI CCTNNNNNAGG 1 cut(s) 864
XbaI TCTAGA 3 cut(s) 1034, 1804, 2692
XceI RCATGY 5 cut(s) 413, 554, 661, 3266, 3313
XcmI CCANNNNNNNNNTGG 2 cut(s) 4540, 4657
XhoI CTCGAG 3 cut(s) 384, 2648, 4072
XmiI GTMKAC 2 cut(s) 267, 512
XmnI GAANNNNTTC 2 cut(s) 675, 2688
ZraI GACGTC 3 cut(s) 51, 222, 3595
Zsp2I ATGCAT 3 cut(s) 1255, 1725, 3649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.