pycom08g15740

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0004594
Physical Location & Seq
Forward (+)
15514831 .. 15517821
2991 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 717 bp
ATGTTGCTAAGTTTGTTCAGATCGATCAAGTCCCCTCTCTGCCAGGAACATGAGTTAGGATTGTTTGCTATTTATGATGGCCATTTGGGAGATAATGTGCCAGCATACCTACAGAAGCATTTATTTTCAAATATCCTTAAGGAGGAAGAGTTTTGGGTTGATCCTAATCGATCTATCTCAAAAGCCTATGAGAGGACAGACCAGGCAATTCTTTCACATAGTTCTGACTTGGGGCGTGGCGGGTCTACTGCTGTCACTGCAATATTGTTAAACGGTCGGCGGTTATTGGTGGCAAATGTTGGAGATTCACGGGCAGTTCTTTCAAGGGCAGGTCTGGCAGTACAGATGTCTATAGATCATGAGCCCAACAACGAACGAGGCAGCATTGAAAACAAAGGTGGCTTTGTCTCTAACATGCCAGGAGATGTCCCTAGAGTCAATGGGCAGCTGGCAGTTTCTCGTGCTTTTGGAGACAAAAGCCTCAAGTCACATTTGCGATCGGATCCCGACATACAAGATGTCTACGTAGACAATAGTACTGATGTTCTAATCCTTGCCAGTGATGGTCTTTGGAAGGTCATGGAGAATCAAGAGGTAGTTAATATGGCAAGAAGGACTAAAGACCCTCTGAAGGCGGCAAAGCAACTAACCGCTGAAGCACTGAAAAGAGACAGTAAAGATGATATTTCATGCGTTGTTGTTAGACTGAGGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

239

Amino Acids

26.21

Weight (kDa)

6.6

Isoelectric Point (pI)

47.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 16 - 229 3.1e-53 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 320
AccI GTMKAC 3 cut(s) 245, 522, 528
AciI CCGC 4 cut(s) 240, 280, 635, 651
AclWI GGATC 3 cut(s) 155, 497, 510
AcoI YGGCCR 1 cut(s) 79
AcuI CTGAAG 2 cut(s) 650, 675
AfaI GTAC 2 cut(s) 342, 538
AfiI CCNNNNNNNGG 3 cut(s) 142, 192, 631
AflII CTTAAG 1 cut(s) 137
AgsI TTSAA 3 cut(s) 129, 324, 389
AjnI CCWGG 3 cut(s) 42, 201, 418
AluBI AGCT 1 cut(s) 448
AluI AGCT 1 cut(s) 448
Alw26I GTCTC 3 cut(s) 412, 465, 663
AlwI GGATC 3 cut(s) 155, 497, 510
AoxI GGCC 1 cut(s) 79
ApeKI GCWGC 2 cut(s) 381, 445
BalI TGGCCA 1 cut(s) 81
BamHI GGATCC 1 cut(s) 502
BanII GRGCYC 1 cut(s) 366
BauI CACGAG 1 cut(s) 459
BbvI GCAGC 2 cut(s) 393, 457
BccI CCATC 2 cut(s) 71, 557
BciT130I CCWGG 3 cut(s) 44, 203, 420
BcoDI GTCTC 3 cut(s) 412, 465, 663
BfaI CTAG 1 cut(s) 432
BfmI CTRYAG 2 cut(s) 110, 351
BfrI CTTAAG 1 cut(s) 137
BfuAI ACCTGC 1 cut(s) 320
BisI GCNGC 3 cut(s) 382, 446, 636
BlsI GCNGC 3 cut(s) 383, 447, 637
BmcAI AGTACT 1 cut(s) 538
Bme1390I CCNGG 3 cut(s) 44, 203, 420
BmiI GGNNCC 1 cut(s) 504
BmrFI CCNGG 3 cut(s) 44, 203, 420
BpuEI CTTGAG 1 cut(s) 467
Bsa29I ATCGAT 2 cut(s) 23, 169
BsaAI YACGTR 1 cut(s) 526
BsaBI GATNNNNATC 1 cut(s) 165
BsaXI ACNNNNNCTCC 4 cut(s) 81, 111, 134, 164
Bsc4I CCNNNNNNNGG 3 cut(s) 142, 192, 631
Bse1I ACTGG 1 cut(s) 558
Bse8I GATNNNNATC 1 cut(s) 165
BseBI CCWGG 3 cut(s) 44, 203, 420
BseCI ATCGAT 2 cut(s) 23, 169
BseJI GATNNNNATC 1 cut(s) 165
BseLI CCNNNNNNNGG 3 cut(s) 142, 192, 631
BseMII CTCAG 1 cut(s) 698
BseNI ACTGG 1 cut(s) 558
BseXI GCAGC 2 cut(s) 393, 457
Bsh1285I CGRYCG 2 cut(s) 277, 500
BshFI GGCC 1 cut(s) 81
BshVI ATCGAT 2 cut(s) 23, 169
BsiEI CGRYCG 2 cut(s) 277, 500
BslFI GGGAC 2 cut(s) 16, 413
BslI CCNNNNNNNGG 3 cut(s) 142, 192, 631
BsmAI GTCTC 3 cut(s) 412, 465, 663
BsmFI GGGAC 2 cut(s) 16, 413
BsnI GGCC 1 cut(s) 81
Bsp1286I GDGCHC 1 cut(s) 366
Bsp143I GATC 7 cut(s) 20, 24, 160, 170, 355, 497, 502
BspACI CCGC 4 cut(s) 240, 280, 635, 651
BspANI GGCC 1 cut(s) 81
BspCNI CTCAG 1 cut(s) 699
BspDI ATCGAT 2 cut(s) 23, 169
BspHI TCATGA 1 cut(s) 358
BspLI GGNNCC 1 cut(s) 504
BspMI ACCTGC 1 cut(s) 320
BspPI GGATC 3 cut(s) 155, 497, 510
BspTI CTTAAG 1 cut(s) 137
BsrI ACTGG 1 cut(s) 558
BssMI GATC 7 cut(s) 20, 24, 160, 170, 355, 497, 502
BssSI CACGAG 1 cut(s) 459
Bst2BI CACGAG 1 cut(s) 459
Bst2UI CCWGG 3 cut(s) 44, 203, 420
Bst4CI ACNGT 2 cut(s) 275, 674
Bst6I CTCTTC 1 cut(s) 141
BstAFI CTTAAG 1 cut(s) 137
BstBAI YACGTR 1 cut(s) 526
BstC8I GCNNGC 2 cut(s) 102, 450
BstDEI CTNAG 2 cut(s) 8, 707
BstENI CCTNNNNNAGG 2 cut(s) 140, 190
BstKTI GATC 7 cut(s) 23, 27, 163, 173, 358, 500, 505
BstMAI GTCTC 3 cut(s) 412, 465, 663
BstMBI GATC 7 cut(s) 20, 24, 160, 170, 355, 497, 502
BstMCI CGRYCG 2 cut(s) 277, 500
BstMWI GCNNNNNNNGC 2 cut(s) 257, 335
BstNI CCWGG 3 cut(s) 44, 203, 420
BstNSI RCATGY 1 cut(s) 418
BstSCI CCNGG 3 cut(s) 42, 201, 418
BstSFI CTRYAG 2 cut(s) 110, 351
BstSNI TACGTA 1 cut(s) 526
BstV1I GCAGC 2 cut(s) 393, 457
BstX2I RGATCY 1 cut(s) 502
BstYI RGATCY 1 cut(s) 502
Bsu15I ATCGAT 2 cut(s) 23, 169
BsuRI GGCC 1 cut(s) 81
BsuTUI ATCGAT 2 cut(s) 23, 169
BtsI GCAGTG 1 cut(s) 255
BtsIMutI CAGTG 3 cut(s) 255, 565, 659
BveI ACCTGC 1 cut(s) 320
Cac8I GCNNGC 2 cut(s) 102, 450
CciI TCATGA 1 cut(s) 358
ClaI ATCGAT 2 cut(s) 23, 169
Csp6I GTAC 2 cut(s) 341, 537
CviAII CATG 5 cut(s) 50, 359, 415, 580, 690
CviJI RGCY 6 cut(s) 81, 185, 364, 402, 448, 480
CviKI_1 RGCY 6 cut(s) 81, 185, 364, 402, 448, 480
CviQI GTAC 2 cut(s) 341, 537
DdeI CTNAG 2 cut(s) 8, 707
DpnI GATC 7 cut(s) 22, 26, 162, 172, 357, 499, 504
DpnII GATC 7 cut(s) 20, 24, 160, 170, 355, 497, 502
EaeI YGGCCR 1 cut(s) 79
Eam1104I CTCTTC 1 cut(s) 141
EarI CTCTTC 1 cut(s) 141
Eco105I TACGTA 1 cut(s) 526
Eco24I GRGCYC 1 cut(s) 366
Eco57I CTGAAG 2 cut(s) 650, 675
EcoNI CCTNNNNNAGG 2 cut(s) 140, 190
EcoRII CCWGG 3 cut(s) 42, 201, 418
EcoT38I GRGCYC 1 cut(s) 366
FaeI CATG 5 cut(s) 53, 362, 418, 583, 693
FaqI GGGAC 2 cut(s) 16, 413
FatI CATG 5 cut(s) 49, 358, 414, 579, 689
FauI CCCGC 1 cut(s) 233
FblI GTMKAC 3 cut(s) 245, 522, 528
Fnu4HI GCNGC 3 cut(s) 382, 446, 636
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 3 cut(s) 382, 446, 636
FspBI CTAG 1 cut(s) 432
GluI GCNGC 3 cut(s) 382, 446, 636
HaeIII GGCC 1 cut(s) 81
Hin1II CATG 5 cut(s) 53, 362, 418, 583, 693
HinfI GANTC 3 cut(s) 305, 435, 586
Hpy166II GTNNAC 3 cut(s) 246, 523, 529
Hpy188I TCNGA 4 cut(s) 20, 226, 502, 630
Hpy188III TCNNGA 3 cut(s) 359, 506, 590
Hpy8I GTNNAC 3 cut(s) 246, 523, 529
HpyAV CCTTC 3 cut(s) 568, 606, 625
HpyCH4III ACNGT 2 cut(s) 275, 674
HpyCH4IV ACGT 1 cut(s) 525
HpyCH4V TGCA 1 cut(s) 260
HpyF10VI GCNNNNNNNGC 2 cut(s) 257, 335
HpyF3I CTNAG 2 cut(s) 8, 707
HpySE526I ACGT 1 cut(s) 525
Hsp92II CATG 5 cut(s) 53, 362, 418, 583, 693
Kzo9I GATC 7 cut(s) 20, 24, 160, 170, 355, 497, 502
Lsp1109I GCAGC 2 cut(s) 393, 457
MaeI CTAG 1 cut(s) 432
MaeII ACGT 1 cut(s) 525
MaeIII GTNAC 2 cut(s) 253, 486
MalI GATC 7 cut(s) 22, 26, 162, 172, 357, 499, 504
MboI GATC 7 cut(s) 20, 24, 160, 170, 355, 497, 502
MboII GAAGA 1 cut(s) 158
MflI RGATCY 1 cut(s) 502
MhlI GDGCHC 1 cut(s) 366
MlsI TGGCCA 1 cut(s) 81
MluCI AATT 1 cut(s) 207
MluNI TGGCCA 1 cut(s) 81
MlyI GAGTC 1 cut(s) 444
MmeI TCCRAC 1 cut(s) 280
MnlI CCTC 8 cut(s) 45, 136, 186, 371, 491, 586, 636, 702
Mox20I TGGCCA 1 cut(s) 81
MscI TGGCCA 1 cut(s) 81
MseI TTAA 3 cut(s) 138, 269, 600
Msp20I TGGCCA 1 cut(s) 81
MspA1I CMGCKG 2 cut(s) 448, 653
MspCI CTTAAG 1 cut(s) 137
MspR9I CCNGG 3 cut(s) 44, 203, 420
MvaI CCWGG 3 cut(s) 44, 203, 420
MwoI GCNNNNNNNGC 2 cut(s) 257, 335
NdeII GATC 7 cut(s) 20, 24, 160, 170, 355, 497, 502
NlaIII CATG 5 cut(s) 53, 362, 418, 583, 693
NlaIV GGNNCC 1 cut(s) 504
NmuCI GTSAC 2 cut(s) 253, 486
NspI RCATGY 1 cut(s) 418
PagI TCATGA 1 cut(s) 358
PfeI GAWTC 2 cut(s) 305, 586
PkrI GCNGC 3 cut(s) 383, 447, 637
Ple19I CGATCG 1 cut(s) 500
PleI GAGTC 1 cut(s) 443
PpsI GAGTC 1 cut(s) 443
Ppu21I YACGTR 1 cut(s) 526
Psp6I CCWGG 3 cut(s) 42, 201, 418
PspGI CCWGG 3 cut(s) 42, 201, 418
PspN4I GGNNCC 1 cut(s) 504
PsuI RGATCY 1 cut(s) 502
PvuI CGATCG 1 cut(s) 500
PvuII CAGCTG 1 cut(s) 448
RsaI GTAC 2 cut(s) 342, 538
RsaNI GTAC 2 cut(s) 341, 537
SaqAI TTAA 3 cut(s) 138, 269, 600
SatI GCNGC 3 cut(s) 382, 446, 636
Sau3AI GATC 7 cut(s) 20, 24, 160, 170, 355, 497, 502
ScaI AGTACT 1 cut(s) 538
SchI GAGTC 1 cut(s) 444
ScrFI CCNGG 3 cut(s) 44, 203, 420
SduI GDGCHC 1 cut(s) 366
SetI ASST 7 cut(s) 111, 334, 400, 450, 528, 579, 597
SfcI CTRYAG 2 cut(s) 110, 351
SmlI CTYRAG 2 cut(s) 137, 482
SmoI CTYRAG 2 cut(s) 137, 482
SnaBI TACGTA 1 cut(s) 526
Sse9I AATT 1 cut(s) 207
SsiI CCGC 4 cut(s) 240, 280, 635, 651
SspI AATATT 1 cut(s) 264
SspMI CTAG 1 cut(s) 432
StyD4I CCNGG 3 cut(s) 42, 201, 418
TaaI ACNGT 2 cut(s) 275, 674
TaiI ACGT 1 cut(s) 528
TaqI TCGA 2 cut(s) 23, 169
TasI AATT 1 cut(s) 207
TatI WGTACW 2 cut(s) 340, 536
TauI GCSGC 1 cut(s) 638
TfiI GAWTC 2 cut(s) 305, 586
Tru1I TTAA 3 cut(s) 138, 269, 600
Tru9I TTAA 3 cut(s) 138, 269, 600
TscAI CASTG 3 cut(s) 262, 565, 666
TseFI GTSAC 2 cut(s) 253, 486
TseI GCWGC 2 cut(s) 381, 445
Tsp45I GTSAC 2 cut(s) 253, 486
TspDTI ATGAA 1 cut(s) 678
TspRI CASTG 3 cut(s) 262, 565, 666
Vha464I CTTAAG 1 cut(s) 137
XagI CCTNNNNNAGG 2 cut(s) 140, 190
XceI RCATGY 1 cut(s) 418
XmiI GTMKAC 3 cut(s) 245, 522, 528
XspI CTAG 1 cut(s) 432
ZrmI AGTACT 1 cut(s) 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.