pycom08g16250

FAM91 N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0004069
Physical Location & Seq
Forward (+)
16131608 .. 16135796
4189 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1269 bp
ATGCAGCGCGCTCCGGCAACGATCGAGGAACAGTTGTTACTGAAAGCAATCAAAGAAGAATGCCCGTGGGAGAGTCTCCCGAAACGGCTTCAAGTCACATTGTCTTCCAAAGAAGAATGGCACAGAAGGGTAATTGAGCATTGCATAAAGAAACGACTCCCATGGAACACTTGTTTTGCTCGGAAAGTGTGCAAAGAAAGTGAATATTATGAAGACATGATGCGGTACTTGCGAAGGAATTTAGCACTATTTCCCTATCACCTTGCGGAGTATGTTTGCCGTGTAATGAGGGTATCCCCTTTCAGATATTACTGCGATATGATTTTTGAAGTTATGAGAAATGAACAACCTTACGACAGCATCCCCAATTTTAGTGCTGCGGATGCCTTGCGCCTTACAGGGATAGGGAGAAACGAATTTATTGATATAATGAACAAGTGCAGATCTAAGAAAATTATGTGGAAGCTGAACAAGTCAATTGCAAAAGAACTTTTACCTACACAACCTGTGGATTTTACAATTGAACCGTGGTGGGGAATTTGTCTCGTGAATTTCACGCTGGAAGAATTTAAGAAACTCTCGGAGGAGGAAACGGCAACAATAGATAAAATATGTAAAGAGGAAGCAAATTCATATATTCTCTTCGATCGATCAGATATTATTAAAGGTCTTCACCAACGAGGATTAGTATACTTTGATGTCCCTGTATATCCTGATGACCGTTTTAAAGTTTCCAGGCTCGAAGGATTTGTTTCGAACAGGGAGCAATCTTATGAAGATCCTATAGAGGAGTTACTATATGCAGTTTTTGTTGTTTCAAGCGAGTATGCAACTGTTGCTGAACTGGCAACAACATTACAGGCTGACCTTTCACAGCTGCAGGCTGCTGCATCTTTTGCATGTCGATTGGGATGGGCGGTAAAATTATTTGATCGATCAGCATCTGTGCTTCAAGATACAAGTTTACCTCGATCTCCTAGAAACAGTCTCAGTGAAGAAGATACTTCTGGTCGTAGTACAAGCTCAGCAATTATGTTTGCCGATGGTGAAGCTAGTCTACAAGGAGATGTTTCGGGGACAGAAAACTCTTTGCATGATCGTGTTGCATTTGTTGTTGATGCTAACATAACATCCTATCTTATGATGGGGTCTGTCTCACCAGGTTTGTTTCTTCTTCTCAAGACCGAATTAGTTTATTATATTGAATACCATCAACTTCTGATCTGCAGTCTGAATTTTGCTGACATACTAGTCATGTGGTTGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAM91_C FAM91_N

Protein Analysis

423

Amino Acids

48.78

Weight (kDa)

5.43

Isoelectric Point (pI)

55.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAM91_N PF14647 17 - 309 4.9e-93 FAM91 N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1250
AccI GTMKAC 2 cut(s) 690, 1057
AccII CGCG 1 cut(s) 9
AciI CCGC 4 cut(s) 223, 266, 380, 917
AclWI GGATC 1 cut(s) 773
AcsI RAATTY 7 cut(s) 238, 416, 537, 550, 566, 628, 1234
AfaI GTAC 2 cut(s) 227, 1018
AfiI CCNNNNNNNGG 1 cut(s) 533
AgsI TTSAA 7 cut(s) 92, 329, 524, 819, 953, 1205, 1264
AhlI ACTAGT 1 cut(s) 1249
AjnI CCWGG 2 cut(s) 734, 1159
AluBI AGCT 4 cut(s) 466, 877, 1023, 1052
AluI AGCT 4 cut(s) 466, 877, 1023, 1052
Alw26I GTCTC 4 cut(s) 80, 548, 992, 1159
AlwI GGATC 1 cut(s) 773
AlwNI CAGNNNCTG 1 cut(s) 944
ApeKI GCWGC 5 cut(s) 4, 377, 877, 884, 887
ApoI RAATTY 7 cut(s) 238, 416, 537, 550, 566, 628, 1234
AspLEI GCGC 3 cut(s) 9, 11, 393
AsuHPI GGTGA 4 cut(s) 251, 665, 1058, 1149
AsuII TTCGAA 1 cut(s) 755
BarI GAAGNNNNNNTAC 2 cut(s) 1041, 1073
BauI CACGAG 1 cut(s) 545
BbsI GAAGAC 3 cut(s) 96, 219, 662
BbvI GCAGC 5 cut(s) 16, 364, 864, 871, 874
BccI CCATC 4 cut(s) 906, 1037, 1138, 1218
BceAI ACGGC 3 cut(s) 101, 264, 609
BciT130I CCWGG 2 cut(s) 736, 1161
BciVI GTATCC 1 cut(s) 304
BcoDI GTCTC 4 cut(s) 80, 548, 992, 1159
BcuI ACTAGT 1 cut(s) 1249
BfaI CTAG 3 cut(s) 978, 1053, 1250
BfmI CTRYAG 3 cut(s) 783, 878, 1225
BfuI GTATCC 1 cut(s) 304
BglII AGATCT 1 cut(s) 443
BisI GCNGC 5 cut(s) 5, 378, 878, 885, 888
BlpI GCTNAGC 1 cut(s) 1024
BlsI GCNGC 5 cut(s) 6, 379, 879, 886, 889
Bme1390I CCNGG 2 cut(s) 736, 1161
BmrFI CCNGG 2 cut(s) 736, 1161
BmsI GCATC 6 cut(s) 210, 369, 373, 899, 950, 1108
BpiI GAAGAC 3 cut(s) 96, 219, 662
Bpu1102I GCTNAGC 1 cut(s) 1024
Bpu14I TTCGAA 1 cut(s) 755
BpuEI CTTGAG 1 cut(s) 1163
Bsa29I ATCGAT 2 cut(s) 649, 934
BsaBI GATNNNNATC 1 cut(s) 940
BsaJI CCNNGG 3 cut(s) 65, 161, 527
Bsc4I CCNNNNNNNGG 1 cut(s) 533
Bse1I ACTGG 1 cut(s) 849
Bse3DI GCAATG 1 cut(s) 139
Bse8I GATNNNNATC 1 cut(s) 940
BseBI CCWGG 2 cut(s) 736, 1161
BseCI ATCGAT 2 cut(s) 649, 934
BseDI CCNNGG 3 cut(s) 65, 161, 527
BseGI GGATG 4 cut(s) 360, 388, 917, 1130
BseJI GATNNNNATC 1 cut(s) 940
BseLI CCNNNNNNNGG 1 cut(s) 533
BseMI GCAATG 1 cut(s) 139
BseMII CTCAG 2 cut(s) 1003, 1038
BseNI ACTGG 1 cut(s) 849
BsePI GCGCGC 1 cut(s) 7
BseRI GAGGAG 2 cut(s) 599, 803
BseXI GCAGC 5 cut(s) 16, 364, 864, 871, 874
BsgI GTGCAG 1 cut(s) 460
Bsh1236I CGCG 1 cut(s) 9
Bsh1285I CGRYCG 2 cut(s) 24, 649
BshVI ATCGAT 2 cut(s) 649, 934
BsiEI CGRYCG 2 cut(s) 24, 649
BsiSI CCGG 1 cut(s) 14
BslFI GGGAC 2 cut(s) 686, 1090
BslI CCNNNNNNNGG 1 cut(s) 533
BsmAI GTCTC 4 cut(s) 80, 548, 992, 1159
BsmFI GGGAC 2 cut(s) 686, 1090
BsmI GAATGC 1 cut(s) 65
Bsp119I TTCGAA 1 cut(s) 755
Bsp1720I GCTNAGC 1 cut(s) 1024
Bsp19I CCATGG 1 cut(s) 161
BspACI CCGC 4 cut(s) 223, 266, 380, 917
BspCNI CTCAG 2 cut(s) 1002, 1037
BspDI ATCGAT 2 cut(s) 649, 934
BspFNI CGCG 1 cut(s) 9
BspMAI CTGCAG 2 cut(s) 882, 1229
BspPI GGATC 1 cut(s) 773
BspT104I TTCGAA 1 cut(s) 755
BsrDI GCAATG 1 cut(s) 139
BsrI ACTGG 1 cut(s) 849
BssECI CCNNGG 3 cut(s) 65, 161, 527
BssHII GCGCGC 1 cut(s) 7
BssNAI GTATAC 1 cut(s) 691
BssSI CACGAG 1 cut(s) 545
BssT1I CCWWGG 1 cut(s) 161
Bst1107I GTATAC 1 cut(s) 691
Bst2BI CACGAG 1 cut(s) 545
Bst2UI CCWGG 2 cut(s) 736, 1161
Bst4CI ACNGT 5 cut(s) 33, 528, 722, 835, 986
Bst6I CTCTTC 1 cut(s) 647
BstAPI GCANNNNNTGC 2 cut(s) 836, 896
BstBI TTCGAA 1 cut(s) 755
BstC8I GCNNGC 2 cut(s) 9, 882
BstDEI CTNAG 3 cut(s) 447, 989, 1024
BstDSI CCRYGG 3 cut(s) 65, 161, 527
BstF5I GGATG 4 cut(s) 360, 388, 917, 1130
BstFNI CGCG 1 cut(s) 9
BstHHI GCGC 3 cut(s) 9, 11, 393
BstMAI GTCTC 4 cut(s) 80, 548, 992, 1159
BstMCI CGRYCG 2 cut(s) 24, 649
BstMWI GCNNNNNNNGC 5 cut(s) 229, 383, 836, 845, 896
BstNI CCWGG 2 cut(s) 736, 1161
BstNSI RCATGY 1 cut(s) 903
BstSCI CCNGG 2 cut(s) 734, 1159
BstSFI CTRYAG 3 cut(s) 783, 878, 1225
BstUI CGCG 1 cut(s) 9
BstV1I GCAGC 5 cut(s) 16, 364, 864, 871, 874
BstV2I GAAGAC 3 cut(s) 96, 219, 662
BstX2I RGATCY 2 cut(s) 443, 778
BstYI RGATCY 2 cut(s) 443, 778
BstZ17I GTATAC 1 cut(s) 691
Bsu15I ATCGAT 2 cut(s) 649, 934
BsuI GTATCC 1 cut(s) 304
BsuTUI ATCGAT 2 cut(s) 649, 934
BtgI CCRYGG 3 cut(s) 65, 161, 527
BtsCI GGATG 4 cut(s) 360, 388, 917, 1130
BtsIMutI CAGTG 1 cut(s) 997
Cac8I GCNNGC 2 cut(s) 9, 882
CaiI CAGNNNCTG 1 cut(s) 944
CfoI GCGC 3 cut(s) 9, 11, 393
ClaI ATCGAT 2 cut(s) 649, 934
CsiI ACCWGGT 1 cut(s) 1159
Csp6I GTAC 2 cut(s) 226, 1017
CviAII CATG 5 cut(s) 162, 217, 900, 1094, 1255
CviJI RGCY 8 cut(s) 88, 466, 739, 863, 877, 884, 1023, 1052
CviKI_1 RGCY 8 cut(s) 88, 466, 739, 863, 877, 884, 1023, 1052
CviQI GTAC 2 cut(s) 226, 1017
DdeI CTNAG 3 cut(s) 447, 989, 1024
DraI TTTAAA 1 cut(s) 727
DrdI GACNNNNNNGTC 1 cut(s) 1250
DseDI GACNNNNNNGTC 1 cut(s) 1250
Eam1104I CTCTTC 1 cut(s) 647
EarI CTCTTC 1 cut(s) 647
Eco130I CCWWGG 1 cut(s) 161
EcoRII CCWGG 2 cut(s) 734, 1159
EcoT14I CCWWGG 1 cut(s) 161
ErhI CCWWGG 1 cut(s) 161
FaeI CATG 5 cut(s) 165, 220, 903, 1097, 1258
FaqI GGGAC 2 cut(s) 686, 1090
FatI CATG 5 cut(s) 161, 216, 899, 1093, 1254
FblI GTMKAC 2 cut(s) 690, 1057
Fnu4HI GCNGC 5 cut(s) 5, 378, 878, 885, 888
FokI GGATG 4 cut(s) 347, 395, 924, 1117
Fsp4HI GCNGC 5 cut(s) 5, 378, 878, 885, 888
FspBI CTAG 3 cut(s) 978, 1053, 1250
GlaI GCGC 3 cut(s) 8, 10, 392
GluI GCNGC 5 cut(s) 5, 378, 878, 885, 888
HapII CCGG 1 cut(s) 14
HhaI GCGC 3 cut(s) 9, 11, 393
Hin1II CATG 5 cut(s) 165, 220, 903, 1097, 1258
Hin6I GCGC 3 cut(s) 7, 9, 391
HinP1I GCGC 3 cut(s) 7, 9, 391
HinfI GANTC 2 cut(s) 73, 156
HpaII CCGG 1 cut(s) 14
HphI GGTGA 4 cut(s) 251, 665, 1058, 1149
Hpy166II GTNNAC 3 cut(s) 691, 965, 1058
Hpy188I TCNGA 6 cut(s) 183, 305, 583, 655, 1221, 1233
Hpy188III TCNNGA 5 cut(s) 79, 547, 713, 953, 1180
Hpy8I GTNNAC 3 cut(s) 691, 965, 1058
HpyAV CCTTC 3 cut(s) 120, 228, 737
HpyCH4III ACNGT 5 cut(s) 33, 528, 722, 835, 986
HpyF10VI GCNNNNNNNGC 5 cut(s) 229, 383, 836, 845, 896
HpyF3I CTNAG 3 cut(s) 447, 989, 1024
Hsp92II CATG 5 cut(s) 165, 220, 903, 1097, 1258
HspAI GCGC 3 cut(s) 7, 9, 391
LmnI GCTCC 2 cut(s) 16, 763
Lsp1109I GCAGC 5 cut(s) 16, 364, 864, 871, 874
LweI GCATC 6 cut(s) 210, 369, 373, 899, 950, 1108
MabI ACCWGGT 1 cut(s) 1159
MaeI CTAG 3 cut(s) 978, 1053, 1250
MaeIII GTNAC 3 cut(s) 36, 94, 792
MfeI CAATTG 2 cut(s) 477, 519
MflI RGATCY 2 cut(s) 443, 778
MlyI GAGTC 2 cut(s) 82, 150
MnlI CCTC 8 cut(s) 19, 282, 577, 580, 613, 674, 781, 978
MseI TTAA 3 cut(s) 570, 663, 726
MslI CAYNNNNRTG 1 cut(s) 1098
MspA1I CMGCKG 1 cut(s) 877
MspI CCGG 1 cut(s) 14
MspR9I CCNGG 2 cut(s) 736, 1161
MunI CAATTG 2 cut(s) 477, 519
Mva1269I GAATGC 1 cut(s) 65
MvaI CCWGG 2 cut(s) 736, 1161
MvnI CGCG 1 cut(s) 9
MwoI GCNNNNNNNGC 5 cut(s) 229, 383, 836, 845, 896
NcoI CCATGG 1 cut(s) 161
NlaIII CATG 5 cut(s) 165, 220, 903, 1097, 1258
NmuCI GTSAC 1 cut(s) 94
NspI RCATGY 1 cut(s) 903
NspV TTCGAA 1 cut(s) 755
PauI GCGCGC 1 cut(s) 7
PctI GAATGC 1 cut(s) 65
PkrI GCNGC 5 cut(s) 6, 379, 879, 886, 889
Ple19I CGATCG 2 cut(s) 24, 649
PleI GAGTC 2 cut(s) 81, 150
PpsI GAGTC 2 cut(s) 81, 150
Psp6I CCWGG 2 cut(s) 734, 1159
PspGI CCWGG 2 cut(s) 734, 1159
PsrI GAACNNNNNNTAC 2 cut(s) 21, 53
PstI CTGCAG 2 cut(s) 882, 1229
PstNI CAGNNNCTG 1 cut(s) 944
PsuI RGATCY 2 cut(s) 443, 778
PteI GCGCGC 1 cut(s) 7
PvuI CGATCG 2 cut(s) 24, 649
PvuII CAGCTG 1 cut(s) 877
RsaI GTAC 2 cut(s) 227, 1018
RsaNI GTAC 2 cut(s) 226, 1017
RseI CAYNNNNRTG 1 cut(s) 1098
SaqAI TTAA 3 cut(s) 570, 663, 726
SatI GCNGC 5 cut(s) 5, 378, 878, 885, 888
SchI GAGTC 2 cut(s) 82, 150
ScrFI CCNGG 2 cut(s) 736, 1161
SexAI ACCWGGT 1 cut(s) 1159
SfaNI GCATC 6 cut(s) 210, 369, 373, 899, 950, 1108
SfcI CTRYAG 3 cut(s) 783, 878, 1225
SfuI TTCGAA 1 cut(s) 755
SmiMI CAYNNNNRTG 1 cut(s) 1098
SmlI CTYRAG 1 cut(s) 1178
SmoI CTYRAG 1 cut(s) 1178
SpeI ACTAGT 1 cut(s) 1249
SsiI CCGC 4 cut(s) 223, 266, 380, 917
SspI AATATT 1 cut(s) 206
SspMI CTAG 3 cut(s) 978, 1053, 1250
StyD4I CCNGG 2 cut(s) 734, 1159
StyI CCWWGG 1 cut(s) 161
TaaI ACNGT 5 cut(s) 33, 528, 722, 835, 986
TaqI TCGA 8 cut(s) 24, 645, 649, 741, 755, 904, 934, 970
TaqII GACCGA 1 cut(s) 1199
TatI WGTACW 1 cut(s) 1016
Tru1I TTAA 3 cut(s) 570, 663, 726
Tru9I TTAA 3 cut(s) 570, 663, 726
TscAI CASTG 1 cut(s) 997
TseFI GTSAC 1 cut(s) 94
TseI GCWGC 5 cut(s) 4, 377, 877, 884, 887
Tsp45I GTSAC 1 cut(s) 94
TspDTI ATGAA 5 cut(s) 225, 357, 446, 621, 789
TspRI CASTG 1 cut(s) 997
XapI RAATTY 7 cut(s) 238, 416, 537, 550, 566, 628, 1234
XceI RCATGY 1 cut(s) 903
XmiI GTMKAC 2 cut(s) 690, 1057
XspI CTAG 3 cut(s) 978, 1053, 1250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.