pycom08g18330

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0015722
Physical Location & Seq
Reverse (-)
18570577 .. 18571844
1268 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 642 bp
ATGGTGGCCTCGTCCTCATCGTTGTTTGAGTACTCCTCGGACTCGTCCTCGTCGTCTTCGTCTGTATCTCGCCGCAGACATCATCCCCACCACCGCAGTCGCCGAGATAAAGACAAAGATGCCCTCAAGATCCGAAAAAAGACCAGTCGCTCCAACACTAAACGACGTCGCAGACACCGCCACCGCCACTCCTCGTCGGATTCTTACTCTTCCTCCTCTCCTTCCGATTACAGGTCAATTTACAAAACCCTTCTTTTATTTTTTGGGCTTATGTATGGTGGCTTTAATTTTGTTATCAGTTTTATCCTCTGTTTGGTTAGGTTGTTGGATTGGTGTTTGTACTATAGAAACTTAGATGTGCTTACCCCCTTTTCATTGACATCCACGGATACAATAGTTGTTAGAGCTTATGAGGCAATAATGGATTGTGTGAGAGCTGTAATTGTTGGGGCGGAGGGTACTCCTTACCATGATGGTCTCTTCTTCTTTGATGTTTGCTTCCCCAGTGGCTATCCCAATGTACCACCAGTATGTGATAATTTTGAACTCCTAGAATTGATTTGTATCTGTTTACATCTTTGGCCTAACCGGGAACTCTGTTATGCGGAATGTCTACTACCACTCTGGTGGCCTTTGATTTAA

Protein Analysis

214

Amino Acids

24.71

Weight (kDa)

8.98

Isoelectric Point (pI)

70.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 140 - 178 5.1e-07 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 169
AccI GTMKAC 1 cut(s) 613
AciI CCGC 6 cut(s) 73, 94, 178, 184, 452, 605
AclWI GGATC 1 cut(s) 124
AcyI GRCGYC 1 cut(s) 166
AfaI GTAC 4 cut(s) 32, 341, 460, 522
AfiI CCNNNNNNNGG 2 cut(s) 231, 313
AgsI TTSAA 1 cut(s) 545
AleI CACNNNNGTG 1 cut(s) 625
AluBI AGCT 2 cut(s) 407, 437
AluI AGCT 2 cut(s) 407, 437
Alw26I GTCTC 1 cut(s) 482
AlwI GGATC 1 cut(s) 124
AoxI GGCC 3 cut(s) 6, 581, 629
AsuC2I CCSGG 1 cut(s) 590
BaeI ACNNNNGTAYC 2 cut(s) 381, 414
BbsI GAAGAC 1 cut(s) 48
BccI CCATC 1 cut(s) 467
BciVI GTATCC 1 cut(s) 382
BcnI CCSGG 1 cut(s) 590
BcoDI GTCTC 1 cut(s) 482
BfaI CTAG 1 cut(s) 551
BfmI CTRYAG 1 cut(s) 343
BfuI GTATCC 1 cut(s) 382
BisI GCNGC 1 cut(s) 73
BlsI GCNGC 1 cut(s) 74
BmcAI AGTACT 1 cut(s) 32
Bme1390I CCNGG 1 cut(s) 590
BmrFI CCNGG 1 cut(s) 590
BmrI ACTGGG 1 cut(s) 498
BmsI GCATC 1 cut(s) 109
BmuI ACTGGG 1 cut(s) 498
BpiI GAAGAC 1 cut(s) 48
BplI GAGNNNNNCTC 2 cut(s) 20, 52
BpuEI CTTGAG 1 cut(s) 110
BpuMI CCSGG 1 cut(s) 590
BsaBI GATNNNNATC 1 cut(s) 563
BsaHI GRCGYC 1 cut(s) 166
BsaI GGTCTC 1 cut(s) 482
BsaJI CCNNGG 2 cut(s) 36, 384
BsaXI ACNNNNNCTCC 4 cut(s) 173, 197, 203, 227
Bsc4I CCNNNNNNNGG 2 cut(s) 231, 313
Bse1I ACTGG 3 cut(s) 144, 504, 527
Bse8I GATNNNNATC 1 cut(s) 563
BseDI CCNNGG 2 cut(s) 36, 384
BseGI GGATG 2 cut(s) 82, 380
BseJI GATNNNNATC 1 cut(s) 563
BseLI CCNNNNNNNGG 2 cut(s) 231, 313
BseNI ACTGG 3 cut(s) 144, 504, 527
BseRI GAGGAG 3 cut(s) 25, 181, 205
BshFI GGCC 3 cut(s) 8, 583, 631
BsiSI CCGG 1 cut(s) 589
BslI CCNNNNNNNGG 2 cut(s) 231, 313
BsmAI GTCTC 1 cut(s) 482
BsnI GGCC 3 cut(s) 8, 583, 631
Bso31I GGTCTC 1 cut(s) 482
Bsp143I GATC 1 cut(s) 129
BspACI CCGC 6 cut(s) 73, 94, 178, 184, 452, 605
BspANI GGCC 3 cut(s) 8, 583, 631
BspPI GGATC 1 cut(s) 124
BspTNI GGTCTC 1 cut(s) 482
BsrI ACTGG 3 cut(s) 144, 504, 527
BssECI CCNNGG 2 cut(s) 36, 384
BssMI GATC 1 cut(s) 129
BssNI GRCGYC 1 cut(s) 166
Bst6I CTCTTC 2 cut(s) 214, 485
BstACI GRCGYC 1 cut(s) 166
BstDEI CTNAG 1 cut(s) 352
BstDSI CCRYGG 1 cut(s) 384
BstF5I GGATG 2 cut(s) 82, 380
BstKTI GATC 1 cut(s) 132
BstMAI GTCTC 1 cut(s) 482
BstMBI GATC 1 cut(s) 129
BstMWI GCNNNNNNNGC 2 cut(s) 177, 413
BstSCI CCNGG 1 cut(s) 588
BstSFI CTRYAG 1 cut(s) 343
BstV2I GAAGAC 1 cut(s) 48
BstX2I RGATCY 1 cut(s) 129
BstXI CCANNNNNNTGG 1 cut(s) 627
BstYI RGATCY 1 cut(s) 129
BsuI GTATCC 1 cut(s) 382
BsuRI GGCC 3 cut(s) 8, 583, 631
BtgI CCRYGG 1 cut(s) 384
BtsCI GGATG 2 cut(s) 82, 380
BtsIMutI CAGTG 1 cut(s) 511
Csp6I GTAC 4 cut(s) 31, 340, 459, 521
CviAII CATG 1 cut(s) 470
CviJI RGCY 8 cut(s) 8, 268, 282, 407, 437, 510, 583, 631
CviKI_1 RGCY 8 cut(s) 8, 268, 282, 407, 437, 510, 583, 631
CviQI GTAC 4 cut(s) 31, 340, 459, 521
DdeI CTNAG 1 cut(s) 352
DpnI GATC 1 cut(s) 131
DpnII GATC 1 cut(s) 129
Eam1104I CTCTTC 2 cut(s) 214, 485
EarI CTCTTC 2 cut(s) 214, 485
EciI GGCGGA 1 cut(s) 467
Eco31I GGTCTC 1 cut(s) 482
FaeI CATG 1 cut(s) 473
FaiI YATR 7 cut(s) 272, 276, 345, 411, 471, 532, 603
FatI CATG 1 cut(s) 469
FblI GTMKAC 1 cut(s) 613
Fnu4HI GCNGC 1 cut(s) 73
FokI GGATG 2 cut(s) 69, 367
Fsp4HI GCNGC 1 cut(s) 73
FspBI CTAG 1 cut(s) 551
GluI GCNGC 1 cut(s) 73
HaeIII GGCC 3 cut(s) 8, 583, 631
HapII CCGG 1 cut(s) 589
Hin1I GRCGYC 1 cut(s) 166
Hin1II CATG 1 cut(s) 473
HinfI GANTC 2 cut(s) 41, 200
HpaII CCGG 1 cut(s) 589
Hpy166II GTNNAC 2 cut(s) 572, 614
Hpy188I TCNGA 4 cut(s) 40, 134, 199, 226
Hpy188III TCNNGA 1 cut(s) 127
Hpy8I GTNNAC 2 cut(s) 572, 614
Hpy99I CGWCG 4 cut(s) 55, 168, 171, 199
HpyAV CCTTC 2 cut(s) 231, 260
HpyCH4IV ACGT 1 cut(s) 166
HpyF10VI GCNNNNNNNGC 2 cut(s) 177, 413
HpyF3I CTNAG 1 cut(s) 352
HpySE526I ACGT 1 cut(s) 166
Hsp92I GRCGYC 1 cut(s) 166
Hsp92II CATG 1 cut(s) 473
Kzo9I GATC 1 cut(s) 129
LmnI GCTCC 1 cut(s) 155
LpnPI CCDG 6 cut(s) 157, 217, 517, 540, 602, 610
LweI GCATC 1 cut(s) 109
MaeI CTAG 1 cut(s) 551
MaeII ACGT 1 cut(s) 166
MalI GATC 1 cut(s) 131
MboI GATC 1 cut(s) 129
MboII GAAGA 4 cut(s) 48, 201, 472, 475
MflI RGATCY 1 cut(s) 129
MluCI AATT 5 cut(s) 237, 286, 441, 538, 554
MlyI GAGTC 1 cut(s) 35
MmeI TCCRAC 3 cut(s) 177, 177, 306
MseI TTAA 2 cut(s) 285, 640
MslI CAYNNNNRTG 2 cut(s) 529, 625
MspI CCGG 1 cut(s) 589
MspR9I CCNGG 1 cut(s) 590
MwoI GCNNNNNNNGC 2 cut(s) 177, 413
NciI CCSGG 1 cut(s) 590
NdeII GATC 1 cut(s) 129
NlaIII CATG 1 cut(s) 473
NmeAIII GCCGAG 1 cut(s) 128
OliI CACNNNNGTG 1 cut(s) 625
PcsI WCGNNNNNNNCGW 3 cut(s) 17, 50, 56
PfeI GAWTC 1 cut(s) 200
PkrI GCNGC 1 cut(s) 74
PleI GAGTC 1 cut(s) 35
PpsI GAGTC 1 cut(s) 35
PsuI RGATCY 1 cut(s) 129
RsaI GTAC 4 cut(s) 32, 341, 460, 522
RsaNI GTAC 4 cut(s) 31, 340, 459, 521
RseI CAYNNNNRTG 2 cut(s) 529, 625
SaqAI TTAA 2 cut(s) 285, 640
SatI GCNGC 1 cut(s) 73
Sau3AI GATC 1 cut(s) 129
ScaI AGTACT 1 cut(s) 32
SchI GAGTC 1 cut(s) 35
ScrFI CCNGG 1 cut(s) 590
SetI ASST 5 cut(s) 169, 236, 323, 409, 439
SfaNI GCATC 1 cut(s) 109
SfcI CTRYAG 1 cut(s) 343
SmiMI CAYNNNNRTG 2 cut(s) 529, 625
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
Sse9I AATT 5 cut(s) 237, 286, 441, 538, 554
SsiI CCGC 6 cut(s) 73, 94, 178, 184, 452, 605
SspMI CTAG 1 cut(s) 551
StyD4I CCNGG 1 cut(s) 588
TaiI ACGT 1 cut(s) 169
TasI AATT 5 cut(s) 237, 286, 441, 538, 554
TatI WGTACW 2 cut(s) 30, 339
TauI GCSGC 1 cut(s) 75
TfiI GAWTC 1 cut(s) 200
Tru1I TTAA 2 cut(s) 285, 640
Tru9I TTAA 2 cut(s) 285, 640
TscAI CASTG 1 cut(s) 511
TspDTI ATGAA 1 cut(s) 363
TspGWI ACGGA 1 cut(s) 401
TspRI CASTG 1 cut(s) 511
XmiI GTMKAC 1 cut(s) 613
XspI CTAG 1 cut(s) 551
ZraI GACGTC 1 cut(s) 167
ZrmI AGTACT 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.