Rh4DG262800

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0015722
Physical Location & Seq
Forward (+)
48144313 .. 48144519
207 bp
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UTR
Exon/CDS
Intron
Rh4DG262800.1

Sequence Viewer

Length: 207 bp
ATGTACAAGACACGTGTCGACCTCGTCAGGGCTGTGATTGTCGGAGCAGACGGCACACCCTACGAGGACGGACTCTTCTTCTTCGACATCAAGTTCCAGGATAACTATCCGAACCAACCACCAGAGGTTCATTATCGCTCGCACGGATTGCAGGTTAACCCTAATATGTATGCGTCTGGACATGTTTGCTTGAGCGCGCCTACTTGA

Protein Analysis

68

Amino Acids

7.69

Weight (kDa)

5.77

Isoelectric Point (pI)

43.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 6 - 65 6.9e-16 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 142
AccI GTMKAC 1 cut(s) 18
AccII CGCG 1 cut(s) 197
AcvI CACGTG 1 cut(s) 14
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 1 cut(s) 28
AflIII ACRYGT 3 cut(s) 11, 13, 181
AjnI CCWGG 1 cut(s) 96
AspLEI GCGC 2 cut(s) 197, 199
BbrPI CACGTG 1 cut(s) 14
BceAI ACGGC 1 cut(s) 67
BciT130I CCWGG 1 cut(s) 98
BfuAI ACCTGC 1 cut(s) 142
Bme1390I CCNGG 1 cut(s) 98
BmrFI CCNGG 1 cut(s) 98
BoxI GACNNNNGTC 1 cut(s) 14
BsaAI YACGTR 1 cut(s) 14
BsaBI GATNNNNATC 1 cut(s) 105
Bsc4I CCNNNNNNNGG 1 cut(s) 28
Bse8I GATNNNNATC 1 cut(s) 105
BseBI CCWGG 1 cut(s) 98
BseJI GATNNNNATC 1 cut(s) 105
BseLI CCNNNNNNNGG 1 cut(s) 28
BsePI GCGCGC 1 cut(s) 195
Bsh1236I CGCG 1 cut(s) 197
BslI CCNNNNNNNGG 1 cut(s) 28
Bsp1407I TGTACA 1 cut(s) 3
BspFNI CGCG 1 cut(s) 197
BspMI ACCTGC 1 cut(s) 142
BsrGI TGTACA 1 cut(s) 3
BssHII GCGCGC 1 cut(s) 195
Bst2UI CCWGG 1 cut(s) 98
Bst6I CTCTTC 1 cut(s) 80
BstAPI GCANNNNNTGC 1 cut(s) 148
BstAUI TGTACA 1 cut(s) 3
BstBAI YACGTR 1 cut(s) 14
BstC8I GCNNGC 2 cut(s) 140, 197
BstFNI CGCG 1 cut(s) 197
BstHHI GCGC 2 cut(s) 197, 199
BstMWI GCNNNNNNNGC 1 cut(s) 148
BstNI CCWGG 1 cut(s) 98
BstNSI RCATGY 1 cut(s) 185
BstPAI GACNNNNGTC 1 cut(s) 14
BstSCI CCNGG 1 cut(s) 96
BstUI CGCG 1 cut(s) 197
BveI ACCTGC 1 cut(s) 142
Cac8I GCNNGC 2 cut(s) 140, 197
CfoI GCGC 2 cut(s) 197, 199
CseI GACGC 1 cut(s) 162
Csp6I GTAC 1 cut(s) 4
CviAII CATG 1 cut(s) 182
CviJI RGCY 1 cut(s) 32
CviKI_1 RGCY 1 cut(s) 32
CviQI GTAC 1 cut(s) 4
Eam1104I CTCTTC 1 cut(s) 80
EarI CTCTTC 1 cut(s) 80
Eco72I CACGTG 1 cut(s) 14
EcoRII CCWGG 1 cut(s) 96
FaeI CATG 1 cut(s) 185
FaiI YATR 3 cut(s) 167, 171, 183
FatI CATG 1 cut(s) 181
FblI GTMKAC 1 cut(s) 18
GlaI GCGC 2 cut(s) 196, 198
HgaI GACGC 1 cut(s) 162
HhaI GCGC 2 cut(s) 197, 199
Hin1II CATG 1 cut(s) 185
Hin6I GCGC 2 cut(s) 195, 197
HinP1I GCGC 2 cut(s) 195, 197
HincII GTYRAC 2 cut(s) 19, 157
HindII GTYRAC 2 cut(s) 19, 157
HinfI GANTC 1 cut(s) 72
HpaI GTTAAC 1 cut(s) 157
Hpy166II GTNNAC 2 cut(s) 19, 157
Hpy188I TCNGA 2 cut(s) 44, 111
Hpy188III TCNNGA 1 cut(s) 177
Hpy8I GTNNAC 2 cut(s) 19, 157
HpyCH4IV ACGT 1 cut(s) 13
HpyCH4V TGCA 1 cut(s) 151
HpyF10VI GCNNNNNNNGC 1 cut(s) 148
HpySE526I ACGT 1 cut(s) 13
Hsp92II CATG 1 cut(s) 185
HspAI GCGC 2 cut(s) 195, 197
KspAI GTTAAC 1 cut(s) 157
LmnI GCTCC 1 cut(s) 44
LpnPI CCDG 6 cut(s) 13, 83, 110, 135, 137, 162
MaeII ACGT 1 cut(s) 13
MboII GAAGA 3 cut(s) 67, 70, 73
MlyI GAGTC 1 cut(s) 66
MmeI TCCRAC 1 cut(s) 22
MnlI CCTC 3 cut(s) 32, 58, 118
MseI TTAA 1 cut(s) 156
MspR9I CCNGG 1 cut(s) 98
MvaI CCWGG 1 cut(s) 98
MvnI CGCG 1 cut(s) 197
MwoI GCNNNNNNNGC 1 cut(s) 148
NlaIII CATG 1 cut(s) 185
NspI RCATGY 1 cut(s) 185
PauI GCGCGC 1 cut(s) 195
PciI ACATGT 1 cut(s) 181
PflFI GACNNNGTC 1 cut(s) 23
PfoI TCCNGGA 1 cut(s) 96
PleI GAGTC 1 cut(s) 66
PmaCI CACGTG 1 cut(s) 14
PmlI CACGTG 1 cut(s) 14
PpsI GAGTC 1 cut(s) 66
Ppu21I YACGTR 1 cut(s) 14
PscI ACATGT 1 cut(s) 181
PshAI GACNNNNGTC 1 cut(s) 14
Psp6I CCWGG 1 cut(s) 96
PspCI CACGTG 1 cut(s) 14
PspGI CCWGG 1 cut(s) 96
PsyI GACNNNGTC 1 cut(s) 23
PteI GCGCGC 1 cut(s) 195
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
SalI GTCGAC 1 cut(s) 17
SaqAI TTAA 1 cut(s) 156
SchI GAGTC 1 cut(s) 66
ScrFI CCNGG 1 cut(s) 98
SetI ASST 4 cut(s) 16, 24, 129, 156
SmlI CTYRAG 1 cut(s) 190
SmoI CTYRAG 1 cut(s) 190
StyD4I CCNGG 1 cut(s) 96
TaiI ACGT 1 cut(s) 16
TaqI TCGA 2 cut(s) 18, 84
TatI WGTACW 1 cut(s) 3
Tru1I TTAA 1 cut(s) 156
Tru9I TTAA 1 cut(s) 156
TspDTI ATGAA 1 cut(s) 119
TspGWI ACGGA 2 cut(s) 84, 159
Tth111I GACNNNGTC 1 cut(s) 23
XceI RCATGY 1 cut(s) 185
XmiI GTMKAC 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.