Rh4CG277800

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0015722
Physical Location & Seq
Forward (+)
53504839 .. 53505171
333 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG277800.1

Sequence Viewer

Length: 333 bp
ATGGCGCCCTTAGTGGAGAGCTTCCACAGATTCGACGTCGTCTCGGACCATTCAGACCACCACTACAGCAACACTGACGGGAACGGAAACAGTTTCACCTACTGTGGAAGCACAGTGTACAAGAAGATCATGCGCGAGTGGAAAATTCTAAGTAAGCACCTCCCGGACTCGACCTATGTGCGAGTGTACAACACTCGAATCGACCTACTGAGAGCCGTCATTGTTGGAGCAGCCAGCACTCCTTACCACGACGGATTGTTCTTCTTCGACATCAAGTTCCCGTCCAACTACCCGTACCAACGCCACAGGTCCATTACCATTCCCACGGATTGA

Protein Analysis

110

Amino Acids

12.78

Weight (kDa)

7.93

Isoelectric Point (pI)

25.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 43 - 100 5.7e-09 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 39
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 1 cut(s) 135
AcsI RAATTY 1 cut(s) 144
AcyI GRCGYC 2 cut(s) 5, 36
AfaI GTAC 3 cut(s) 119, 188, 296
AluBI AGCT 1 cut(s) 21
AluI AGCT 1 cut(s) 21
Alw26I GTCTC 1 cut(s) 46
ApeKI GCWGC 1 cut(s) 230
ApoI RAATTY 1 cut(s) 144
AspLEI GCGC 2 cut(s) 7, 135
AspS9I GGNCC 2 cut(s) 46, 309
AsuC2I CCSGG 1 cut(s) 164
AsuHPI GGTGA 1 cut(s) 88
AvaII GGWCC 2 cut(s) 46, 309
BanI GGYRCC 1 cut(s) 4
BbvI GCAGC 1 cut(s) 242
BceAI ACGGC 1 cut(s) 200
BcgI CGANNNNNNTGC 2 cut(s) 160, 194
BcnI CCSGG 1 cut(s) 164
BcoDI GTCTC 1 cut(s) 46
BfmI CTRYAG 1 cut(s) 64
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 1 cut(s) 231
BlsI GCNGC 1 cut(s) 232
Bme1390I CCNGG 1 cut(s) 164
Bme18I GGWCC 2 cut(s) 46, 309
BmgT120I GGNCC 2 cut(s) 46, 309
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 164
BpuMI CCSGG 1 cut(s) 164
BsaHI GRCGYC 2 cut(s) 5, 36
BsaJI CCNNGG 1 cut(s) 324
BseDI CCNNGG 1 cut(s) 324
BseMII CTCAG 1 cut(s) 200
BseXI GCAGC 1 cut(s) 242
Bsh1236I CGCG 1 cut(s) 135
BshNI GGYRCC 1 cut(s) 4
BsiSI CCGG 1 cut(s) 164
BsmAI GTCTC 1 cut(s) 46
BsmBI CGTCTC 1 cut(s) 46
Bsp1407I TGTACA 2 cut(s) 117, 186
Bsp143I GATC 1 cut(s) 126
BspCNI CTCAG 1 cut(s) 201
BspFNI CGCG 1 cut(s) 135
BspLI GGNNCC 1 cut(s) 6
BspT107I GGYRCC 1 cut(s) 4
BsrGI TGTACA 2 cut(s) 117, 186
BssECI CCNNGG 1 cut(s) 324
BssMI GATC 1 cut(s) 126
BssNI GRCGYC 2 cut(s) 5, 36
Bst4CI ACNGT 3 cut(s) 92, 104, 115
BstACI GRCGYC 2 cut(s) 5, 36
BstAUI TGTACA 2 cut(s) 117, 186
BstC8I GCNNGC 1 cut(s) 235
BstDEI CTNAG 3 cut(s) 10, 149, 209
BstDSI CCRYGG 1 cut(s) 324
BstFNI CGCG 1 cut(s) 135
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 135
BstKTI GATC 1 cut(s) 129
BstMAI GTCTC 1 cut(s) 46
BstMBI GATC 1 cut(s) 126
BstSCI CCNGG 1 cut(s) 162
BstSFI CTRYAG 1 cut(s) 64
BstUI CGCG 1 cut(s) 135
BstV1I GCAGC 1 cut(s) 242
BtgI CCRYGG 1 cut(s) 324
BtsIMutI CAGTG 2 cut(s) 72, 120
Cac8I GCNNGC 1 cut(s) 235
CfoI GCGC 2 cut(s) 7, 135
Cfr13I GGNCC 2 cut(s) 46, 309
Csp6I GTAC 3 cut(s) 118, 187, 295
CviAII CATG 1 cut(s) 130
CviJI RGCY 3 cut(s) 21, 215, 233
CviKI_1 RGCY 3 cut(s) 21, 215, 233
CviQI GTAC 3 cut(s) 118, 187, 295
DdeI CTNAG 3 cut(s) 10, 149, 209
DinI GGCGCC 1 cut(s) 6
DpnI GATC 1 cut(s) 128
DpnII GATC 1 cut(s) 126
Eco47I GGWCC 2 cut(s) 46, 309
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 46
FaeI CATG 1 cut(s) 133
FaiI YATR 2 cut(s) 131, 177
FatI CATG 1 cut(s) 129
Fnu4HI GCNGC 1 cut(s) 231
Fsp4HI GCNGC 1 cut(s) 231
GlaI GCGC 2 cut(s) 6, 134
GluI GCNGC 1 cut(s) 231
HaeII RGCGCY 1 cut(s) 8
HapII CCGG 1 cut(s) 164
HhaI GCGC 2 cut(s) 7, 135
Hin1I GRCGYC 2 cut(s) 5, 36
Hin1II CATG 1 cut(s) 133
Hin6I GCGC 2 cut(s) 5, 133
HinP1I GCGC 2 cut(s) 5, 133
HinfI GANTC 3 cut(s) 30, 167, 198
HpaII CCGG 1 cut(s) 164
HphI GGTGA 1 cut(s) 88
Hpy166II GTNNAC 2 cut(s) 118, 187
Hpy188I TCNGA 2 cut(s) 46, 55
Hpy8I GTNNAC 2 cut(s) 118, 187
Hpy99I CGWCG 3 cut(s) 38, 41, 254
HpyCH4III ACNGT 3 cut(s) 92, 104, 115
HpyCH4IV ACGT 1 cut(s) 36
HpyF3I CTNAG 3 cut(s) 10, 149, 209
HpySE526I ACGT 1 cut(s) 36
Hsp92I GRCGYC 2 cut(s) 5, 36
Hsp92II CATG 1 cut(s) 133
HspAI GCGC 2 cut(s) 5, 133
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 1 cut(s) 126
LmnI GCTCC 1 cut(s) 227
LpnPI CCDG 3 cut(s) 177, 247, 292
Lsp1109I GCAGC 1 cut(s) 242
MaeII ACGT 1 cut(s) 36
MalI GATC 1 cut(s) 128
MboI GATC 1 cut(s) 126
MboII GAAGA 3 cut(s) 136, 253, 256
MluCI AATT 1 cut(s) 144
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 161
MmeI TCCRAC 2 cut(s) 205, 309
MnlI CCTC 1 cut(s) 170
MspI CCGG 1 cut(s) 164
MspR9I CCNGG 1 cut(s) 164
MvnI CGCG 1 cut(s) 135
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 164
NdeII GATC 1 cut(s) 126
NlaIII CATG 1 cut(s) 133
NlaIV GGNNCC 1 cut(s) 6
PfeI GAWTC 2 cut(s) 30, 198
PflFI GACNNNGTC 1 cut(s) 38
PfoI TCCNGGA 1 cut(s) 162
PkrI GCNGC 1 cut(s) 232
PleI GAGTC 1 cut(s) 161
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 161
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 2 cut(s) 46, 309
PsyI GACNNNGTC 1 cut(s) 38
RsaI GTAC 3 cut(s) 119, 188, 296
RsaNI GTAC 3 cut(s) 118, 187, 295
SatI GCNGC 1 cut(s) 231
Sau3AI GATC 1 cut(s) 126
Sau96I GGNCC 2 cut(s) 46, 309
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 1 cut(s) 164
SetI ASST 7 cut(s) 23, 39, 101, 162, 176, 207, 311
SfcI CTRYAG 1 cut(s) 64
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 2 cut(s) 46, 309
Sse9I AATT 1 cut(s) 144
SspDI GGCGCC 1 cut(s) 4
StyD4I CCNGG 1 cut(s) 162
TaaI ACNGT 3 cut(s) 92, 104, 115
TaiI ACGT 1 cut(s) 39
TaqI TCGA 5 cut(s) 33, 170, 196, 201, 267
TasI AATT 1 cut(s) 144
TatI WGTACW 2 cut(s) 117, 186
TfiI GAWTC 2 cut(s) 30, 198
TscAI CASTG 2 cut(s) 79, 120
TseI GCWGC 1 cut(s) 230
TspGWI ACGGA 2 cut(s) 99, 267
TspRI CASTG 2 cut(s) 79, 120
Tth111I GACNNNGTC 1 cut(s) 38
VpaK11BI GGWCC 2 cut(s) 46, 309
XapI RAATTY 1 cut(s) 144
ZraI GACGTC 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.