pycom08g18450

11-beta-hydroxysteroid dehydrogenase-like 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0013147
Physical Location & Seq
Forward (+)
18631789 .. 18633936
2148 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 729 bp
ATGTTGGCACGAAGAGAGAACAGACTGCGAGTGATCAGTGAGAATGCAAGGCTTATAGGTGCAAAGCATGTCATGATTATAGCTGCAGATGTTGTCAAGGAAGAGGAATGCAGGAGATTTATCAGTGAAACAATAAGCTTCCATGGCCGAGTGGATCACCTTGTAAATACAGTAAGTCTAGGACACACATTCTACTTCGAGGAAGTTACAGACACGTCTGTCTTTCCCCATTTGTTGGCAGCAAAGGCAGCTCTGGTTAACTTTTATGAGACCTTGAGATTAGAAGTAAACCATGAGGTAGGGATAACAATTGCAACTCACGGATGGATTGAAAGTGAGATGACAAGAGGCAAGTTCATGCTAGAGGAAGGCGCCGAAATGCAATGGAAAGAAGAAAGGGAGGTACTGGTACATGTGATCGGTGGGCCTGTGGAAGATTTTGCCAGGTTGATTGTAGCTGGGGCTTGTCGAGGAGATTCGTACGTTAAGTATCCAAGCTGGTATGACATATTCCTGCTGTACAGGGTGTTTGTGCCTAACACCCTGCATTGGACATTCCGCCTCATTCTTCCAACGCACGGTGGAAGGAGGACTTCCCTTGTGGGCACCGGGAGGCCTATTTCTGAAGGCTATGGAAGGCCTTTGTTGGAAGGCACTCCTCCAAGGAGATTTGTTGGTCCCAACACAATTTCCCAACAGAGTCCCTCGCGTCCGCTAAAAATGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

243

Amino Acids

27.57

Weight (kDa)

7.18

Isoelectric Point (pI)

47.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013147)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10020
fragaria_vesca FvH4_5g33170
malus_domestica MD08G1214700.v1.1 MD15G1397600.v1.1
prunus_persica Prupe.1G546200_v2.0.a1 Prupe.1G546200_v2.0.a1
pyrus_communis pycom08g18450
rosa_chinensis RchiOBHm_Chr7g0231461
rosa_laevigata RLG00000001417
rosa_multiflora Rmu_sc0001377.1_g000004
rosa_roxburghii Rroxscaffold_3G00229250
rosa_rugosa Rorug07G0266600 Rorug07G0266700
rosa_samantha Rh7AG421100 Rh7BG395600 Rh7CG441200 Rh7DG413600
rosa_wichuraiana Rw7G034750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 218
AccB1I GGYRCC 2 cut(s) 371, 605
AccB7I CCANNNNNTGG 1 cut(s) 235
AccII CGCG 1 cut(s) 709
AciI CCGC 2 cut(s) 559, 713
AclWI GGATC 1 cut(s) 162
AcoI YGGCCR 1 cut(s) 145
AcuI CTGAAG 1 cut(s) 645
AcyI GRCGYC 1 cut(s) 372
AfaI GTAC 4 cut(s) 405, 411, 482, 521
AfiI CCNNNNNNNGG 3 cut(s) 235, 549, 578
AflIII ACRYGT 2 cut(s) 213, 412
AgsI TTSAA 1 cut(s) 332
AjiI CACGTC 1 cut(s) 216
AjnI CCWGG 1 cut(s) 443
AluBI AGCT 5 cut(s) 83, 138, 251, 458, 498
AluI AGCT 5 cut(s) 83, 138, 251, 458, 498
Alw26I GTCTC 1 cut(s) 263
AlwI GGATC 1 cut(s) 162
AoxI GGCC 4 cut(s) 145, 425, 614, 638
ApeKI GCWGC 3 cut(s) 83, 239, 248
AspLEI GCGC 1 cut(s) 374
AspS9I GGNCC 2 cut(s) 425, 677
AsuC2I CCSGG 1 cut(s) 610
AsuHPI GGTGA 1 cut(s) 149
AvaII GGWCC 1 cut(s) 677
BaeGI GKGCMC 1 cut(s) 608
BanI GGYRCC 2 cut(s) 371, 605
BbvI GCAGC 3 cut(s) 70, 251, 260
BccI CCATC 1 cut(s) 318
BciT130I CCWGG 1 cut(s) 445
BciVI GTATCC 1 cut(s) 501
BclI TGATCA 1 cut(s) 33
BcnI CCSGG 1 cut(s) 610
BcoDI GTCTC 1 cut(s) 263
BfaI CTAG 2 cut(s) 179, 362
BfmI CTRYAG 1 cut(s) 84
BfoI RGCGCY 1 cut(s) 375
BfuI GTATCC 1 cut(s) 501
BisI GCNGC 3 cut(s) 84, 240, 249
BlsI GCNGC 3 cut(s) 85, 241, 250
Bme1390I CCNGG 2 cut(s) 445, 610
Bme18I GGWCC 1 cut(s) 677
BmgBI CACGTC 1 cut(s) 216
BmgT120I GGNCC 2 cut(s) 425, 677
BmiI GGNNCC 3 cut(s) 373, 607, 679
BmrFI CCNGG 2 cut(s) 445, 610
BpuEI CTTGAG 1 cut(s) 295
BpuMI CCSGG 1 cut(s) 610
BsaHI GRCGYC 1 cut(s) 372
BsaI GGTCTC 1 cut(s) 263
BsaJI CCNNGG 2 cut(s) 142, 662
Bsc4I CCNNNNNNNGG 3 cut(s) 235, 549, 578
Bse1I ACTGG 1 cut(s) 411
Bse3DI GCAATG 1 cut(s) 389
BseBI CCWGG 1 cut(s) 445
BseDI CCNNGG 2 cut(s) 142, 662
BseGI GGATG 1 cut(s) 329
BseLI CCNNNNNNNGG 3 cut(s) 235, 549, 578
BseMI GCAATG 1 cut(s) 389
BseNI ACTGG 1 cut(s) 411
BseRI GAGGAG 2 cut(s) 486, 648
BseSI GKGCMC 1 cut(s) 608
BseXI GCAGC 3 cut(s) 70, 251, 260
BseYI CCCAGC 1 cut(s) 458
Bsh1236I CGCG 1 cut(s) 709
BshFI GGCC 4 cut(s) 147, 427, 616, 640
BshNI GGYRCC 2 cut(s) 371, 605
BsiSI CCGG 1 cut(s) 609
BsiWI CGTACG 1 cut(s) 480
BslFI GGGAC 2 cut(s) 663, 687
BslI CCNNNNNNNGG 3 cut(s) 235, 549, 578
BsmAI GTCTC 1 cut(s) 263
BsmFI GGGAC 2 cut(s) 663, 687
BsmI GAATGC 2 cut(s) 49, 113
BsnI GGCC 4 cut(s) 147, 427, 616, 640
Bso31I GGTCTC 1 cut(s) 263
Bsp1286I GDGCHC 1 cut(s) 608
Bsp1407I TGTACA 1 cut(s) 519
Bsp143I GATC 3 cut(s) 33, 154, 417
Bsp19I CCATGG 1 cut(s) 142
BspACI CCGC 2 cut(s) 559, 713
BspANI GGCC 4 cut(s) 147, 427, 616, 640
BspFNI CGCG 1 cut(s) 709
BspHI TCATGA 1 cut(s) 72
BspLI GGNNCC 3 cut(s) 373, 607, 679
BspMAI CTGCAG 1 cut(s) 88
BspPI GGATC 1 cut(s) 162
BspT107I GGYRCC 2 cut(s) 371, 605
BspTNI GGTCTC 1 cut(s) 263
BsrDI GCAATG 1 cut(s) 389
BsrGI TGTACA 1 cut(s) 519
BsrI ACTGG 1 cut(s) 411
BssECI CCNNGG 2 cut(s) 142, 662
BssMI GATC 3 cut(s) 33, 154, 417
BssNI GRCGYC 1 cut(s) 372
BssT1I CCWWGG 2 cut(s) 142, 662
Bst2UI CCWGG 1 cut(s) 445
Bst4CI ACNGT 2 cut(s) 172, 581
Bst6I CTCTTC 2 cut(s) 7, 96
BstACI GRCGYC 1 cut(s) 372
BstAUI TGTACA 1 cut(s) 519
BstDSI CCRYGG 1 cut(s) 142
BstF5I GGATG 1 cut(s) 329
BstFNI CGCG 1 cut(s) 709
BstH2I RGCGCY 1 cut(s) 375
BstHHI GCGC 1 cut(s) 374
BstKTI GATC 3 cut(s) 36, 157, 420
BstMAI GTCTC 1 cut(s) 263
BstMBI GATC 3 cut(s) 33, 154, 417
BstMWI GCNNNNNNNGC 3 cut(s) 144, 245, 248
BstNI CCWGG 1 cut(s) 445
BstNSI RCATGY 2 cut(s) 71, 416
BstSCI CCNGG 2 cut(s) 443, 608
BstSFI CTRYAG 1 cut(s) 84
BstSLI GKGCMC 1 cut(s) 608
BstUI CGCG 1 cut(s) 709
BstV1I GCAGC 3 cut(s) 70, 251, 260
BsuI GTATCC 1 cut(s) 501
BsuRI GGCC 4 cut(s) 147, 427, 616, 640
BtgI CCRYGG 1 cut(s) 142
BtrI CACGTC 1 cut(s) 216
BtsCI GGATG 1 cut(s) 329
BtsIMutI CAGTG 2 cut(s) 43, 130
CciI TCATGA 1 cut(s) 72
CfoI GCGC 1 cut(s) 374
Cfr13I GGNCC 2 cut(s) 425, 677
CseI GACGC 1 cut(s) 698
Csp6I GTAC 4 cut(s) 404, 410, 481, 520
CviAII CATG 6 cut(s) 68, 73, 143, 293, 358, 413
CviQI GTAC 4 cut(s) 404, 410, 481, 520
DinI GGCGCC 1 cut(s) 373
DpnI GATC 3 cut(s) 35, 156, 419
DpnII GATC 3 cut(s) 33, 154, 417
DrdI GACNNNNNNGTC 1 cut(s) 218
DseDI GACNNNNNNGTC 1 cut(s) 218
EaeI YGGCCR 1 cut(s) 145
Eam1104I CTCTTC 2 cut(s) 7, 96
EarI CTCTTC 2 cut(s) 7, 96
EciI GGCGGA 1 cut(s) 548
Eco130I CCWWGG 2 cut(s) 142, 662
Eco147I AGGCCT 2 cut(s) 616, 640
Eco31I GGTCTC 1 cut(s) 263
Eco47I GGWCC 1 cut(s) 677
Eco57I CTGAAG 1 cut(s) 645
EcoRII CCWGG 1 cut(s) 443
EcoT14I CCWWGG 2 cut(s) 142, 662
EgeI GGCGCC 1 cut(s) 373
EheI GGCGCC 1 cut(s) 373
ErhI CCWWGG 2 cut(s) 142, 662
FaeI CATG 6 cut(s) 71, 76, 146, 296, 361, 416
FalI AAGNNNNNCTT 2 cut(s) 577, 609
FaqI GGGAC 2 cut(s) 663, 687
FatI CATG 6 cut(s) 67, 72, 142, 292, 357, 412
FbaI TGATCA 1 cut(s) 33
Fnu4HI GCNGC 3 cut(s) 84, 240, 249
FokI GGATG 1 cut(s) 336
Fsp4HI GCNGC 3 cut(s) 84, 240, 249
FspBI CTAG 2 cut(s) 179, 362
GlaI GCGC 1 cut(s) 373
GluI GCNGC 3 cut(s) 84, 240, 249
GsaI CCCAGC 1 cut(s) 462
HaeII RGCGCY 1 cut(s) 375
HaeIII GGCC 4 cut(s) 147, 427, 616, 640
HapII CCGG 1 cut(s) 609
HgaI GACGC 1 cut(s) 698
HhaI GCGC 1 cut(s) 374
Hin1I GRCGYC 1 cut(s) 372
Hin1II CATG 6 cut(s) 71, 76, 146, 296, 361, 416
Hin6I GCGC 1 cut(s) 372
HinP1I GCGC 1 cut(s) 372
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HindIII AAGCTT 1 cut(s) 136
HinfI GANTC 2 cut(s) 476, 700
HpaI GTTAAC 1 cut(s) 259
HpaII CCGG 1 cut(s) 609
HphI GGTGA 1 cut(s) 149
Hpy166II GTNNAC 2 cut(s) 259, 289
Hpy188I TCNGA 1 cut(s) 625
Hpy188III TCNNGA 1 cut(s) 73
Hpy8I GTNNAC 2 cut(s) 259, 289
HpyAV CCTTC 5 cut(s) 362, 579, 620, 630, 644
HpyCH4III ACNGT 2 cut(s) 172, 581
HpyCH4IV ACGT 2 cut(s) 215, 483
HpyCH4V TGCA 7 cut(s) 47, 62, 86, 111, 314, 382, 547
HpyF10VI GCNNNNNNNGC 3 cut(s) 144, 245, 248
HpySE526I ACGT 2 cut(s) 215, 483
Hsp92I GRCGYC 1 cut(s) 372
Hsp92II CATG 6 cut(s) 71, 76, 146, 296, 361, 416
HspAI GCGC 1 cut(s) 372
KasI GGCGCC 1 cut(s) 371
Ksp22I TGATCA 1 cut(s) 33
KspAI GTTAAC 1 cut(s) 259
Kzo9I GATC 3 cut(s) 33, 154, 417
Lsp1109I GCAGC 3 cut(s) 70, 251, 260
MaeI CTAG 2 cut(s) 179, 362
MaeII ACGT 2 cut(s) 215, 483
MaeIII GTNAC 1 cut(s) 205
MalI GATC 3 cut(s) 35, 156, 419
MboI GATC 3 cut(s) 33, 154, 417
MboII GAAGA 5 cut(s) 24, 113, 404, 446, 560
MfeI CAATTG 1 cut(s) 309
MhlI GDGCHC 1 cut(s) 608
MluCI AATT 2 cut(s) 309, 687
Mly113I GGCGCC 1 cut(s) 372
MlyI GAGTC 1 cut(s) 709
MmeI TCCRAC 2 cut(s) 596, 627
MseI TTAA 2 cut(s) 258, 486
MspI CCGG 1 cut(s) 609
MspR9I CCNGG 2 cut(s) 445, 610
MunI CAATTG 1 cut(s) 309
Mva1269I GAATGC 2 cut(s) 49, 113
MvaI CCWGG 1 cut(s) 445
MvnI CGCG 1 cut(s) 709
MwoI GCNNNNNNNGC 3 cut(s) 144, 245, 248
NarI GGCGCC 1 cut(s) 372
NciI CCSGG 1 cut(s) 610
NcoI CCATGG 1 cut(s) 142
NdeII GATC 3 cut(s) 33, 154, 417
NlaIII CATG 6 cut(s) 71, 76, 146, 296, 361, 416
NlaIV GGNNCC 3 cut(s) 373, 607, 679
NmeAIII GCCGAG 1 cut(s) 173
NspI RCATGY 2 cut(s) 71, 416
PagI TCATGA 1 cut(s) 72
PceI AGGCCT 2 cut(s) 616, 640
PciI ACATGT 1 cut(s) 412
PctI GAATGC 2 cut(s) 49, 113
PfeI GAWTC 1 cut(s) 476
Pfl23II CGTACG 1 cut(s) 480
PflMI CCANNNNNTGG 1 cut(s) 235
PkrI GCNGC 3 cut(s) 85, 241, 250
PleI GAGTC 1 cut(s) 708
PluTI GGCGCC 1 cut(s) 375
PpsI GAGTC 1 cut(s) 708
PscI ACATGT 1 cut(s) 412
Psp6I CCWGG 1 cut(s) 443
PspFI CCCAGC 1 cut(s) 458
PspGI CCWGG 1 cut(s) 443
PspLI CGTACG 1 cut(s) 480
PspN4I GGNNCC 3 cut(s) 373, 607, 679
PspPI GGNCC 2 cut(s) 425, 677
PstI CTGCAG 1 cut(s) 88
RsaI GTAC 4 cut(s) 405, 411, 482, 521
RsaNI GTAC 4 cut(s) 404, 410, 481, 520
SaqAI TTAA 2 cut(s) 258, 486
SatI GCNGC 3 cut(s) 84, 240, 249
Sau3AI GATC 3 cut(s) 33, 154, 417
Sau96I GGNCC 2 cut(s) 425, 677
SchI GAGTC 1 cut(s) 709
ScrFI CCNGG 2 cut(s) 445, 610
SduI GDGCHC 1 cut(s) 608
SfcI CTRYAG 1 cut(s) 84
SfoI GGCGCC 1 cut(s) 373
SinI GGWCC 1 cut(s) 677
SmlI CTYRAG 1 cut(s) 274
SmoI CTYRAG 1 cut(s) 274
Sse9I AATT 2 cut(s) 309, 687
SseBI AGGCCT 2 cut(s) 616, 640
SsiI CCGC 2 cut(s) 559, 713
SspDI GGCGCC 1 cut(s) 371
SspMI CTAG 2 cut(s) 179, 362
StuI AGGCCT 2 cut(s) 616, 640
StyD4I CCNGG 2 cut(s) 443, 608
StyI CCWWGG 2 cut(s) 142, 662
TaaI ACNGT 2 cut(s) 172, 581
TaiI ACGT 2 cut(s) 218, 486
TaqI TCGA 2 cut(s) 198, 469
TasI AATT 2 cut(s) 309, 687
TatI WGTACW 1 cut(s) 519
TfiI GAWTC 1 cut(s) 476
Tru1I TTAA 2 cut(s) 258, 486
Tru9I TTAA 2 cut(s) 258, 486
TscAI CASTG 2 cut(s) 43, 130
TseI GCWGC 3 cut(s) 83, 239, 248
TspDTI ATGAA 1 cut(s) 346
TspGWI ACGGA 1 cut(s) 336
TspRI CASTG 2 cut(s) 43, 130
Van91I CCANNNNNTGG 1 cut(s) 235
VpaK11BI GGWCC 1 cut(s) 677
XceI RCATGY 2 cut(s) 71, 416
XspI CTAG 2 cut(s) 179, 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.