pycom08g21360

Belongs to the membrane-bound acyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0004143
Physical Location & Seq
Reverse (-)
20857837 .. 20865608
7772 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1326 bp
ATGAGGACAAGCTTTCATTTAAAAGCGAGAGGGATGTCCATTATTTGGATTTTGATTTCTTTCACATACCTAGCATATCTGCATGGAGCCTGCATTCTATTTGTCCTCTCAATCGCTTCAATGAATTTTCTCTTGGTAAAGATATTTGCACGAACAAAATATTTCCCTTTTGTAGTATGGATTTTCAACATATTCTTTCTTCTGTGCAATCGTGTTTATGAAGGATATTCATTCTCCACAATTGGGCAACGCTGGGCATATTTGGACAACTTTCGGGGCACCTTTAGATGGCACATTTGCTTTAACTTTGTTGTTTTGCGGATGATAAGCTTTGGGTATGATTACCATTGGGCCCACCGAGATTCTCGTTTTGACCATAAGAAACACATTCAGCGCTGCAATATTTGTAAATCAGGGAAAACTTGTTACCATGTTTTACAGGAGAGAGGTGTCCAGAATGACAAGTATGCCTTTACGACGTACCTTGCTTATTTGGTGTATGCACCTCTTTATCTTACTGGGCCGATTGTTAGCTTCAATGCATTTGCCTCACAGTTGGATGTTCCTCAAGATAACTTTTCAGCTAAAGACATTGCTTGGTATGGTTTGCGCTGGGTGTTCAGTCTTCTTCTGATGGAACTAATGACACATCTTTTCTATTACAATGCGTTTGCAATCAGTGGCATGTGGAAAGAGTTACATCCTATGGATGTGTTTATTATTGGATATGGGGTCTTAATGTTCATGTGGCTAAAGTTTTTTCTCATATGGCGCTATTTCCGGTTCTGGTCACTGATATGTGGCATTGAGGTCCCGGAGAATATGCCGAGGTGTATAAATAATTGCTACAACTTGGAAACTTTTTGGAAGAACTGGCATGCTTCCTATAACAAATGGCTTGTGAGGTATTTGTACATTCCTCTTGGGGGCACTCGGAGAAAGCTTCTCAATGTCTGGGTTGTATTCACATTTGTTGCCATTTGGCATGATCTAGAATGGAAGCTTCTTTCATGGGCATGGCTGACTTGCTTATTCTTTATTCCGGAAATGATACTGAAATCAGCAACCAATGCATTTCAGTTCAAGAGTACTCATGGGGAGTTTGTTGTTCGTGAACTTAGTGCGGTAGCTGGTGCAATCACGATCACTTGCCTCATGGTAGCAAACCTTGTTGGTTTTGTTATTGGACCATCTGGGATTAGTTGGTTGATTTCTCAATTCCTTAAACGAGAAGGACTCCCCGTTTTGGCTGGCATGCTTTTGACATTTTATGTAGGGACAAAGCTTATGTTCCACATCCGTGATGCAAAGCAAAGTAGCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MBOAT

Protein Analysis

442

Amino Acids

52.11

Weight (kDa)

9.46

Isoelectric Point (pI)

35.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBOAT PF03062 102 - 363 2e-40 MBOAT, membrane-bound O-acyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 278
AccB7I CCANNNNNTGG 1 cut(s) 45
AccIII TCCGGA 1 cut(s) 1042
AciI CCGC 2 cut(s) 319, 1124
AcsI RAATTY 1 cut(s) 124
AfaI GTAC 3 cut(s) 482, 914, 1090
AfeI AGCGCT 1 cut(s) 395
AfiI CCNNNNNNNGG 5 cut(s) 45, 243, 288, 926, 1246
AgsI TTSAA 4 cut(s) 120, 187, 538, 1084
AjuI GAANNNNNNNTTGG 2 cut(s) 116, 148
AleI CACNNNNGTG 1 cut(s) 1299
AluBI AGCT 9 cut(s) 12, 330, 534, 584, 943, 1003, 1130, 1285, 1323
AluI AGCT 9 cut(s) 12, 330, 534, 584, 943, 1003, 1130, 1285, 1323
Aor13HI TCCGGA 1 cut(s) 1042
Aor51HI AGCGCT 1 cut(s) 395
AoxI GGCC 2 cut(s) 351, 521
ApaI GGGCCC 1 cut(s) 355
ApeKI GCWGC 2 cut(s) 396, 1320
ApoI RAATTY 1 cut(s) 124
AspLEI GCGC 3 cut(s) 396, 612, 774
AspS9I GGNCC 5 cut(s) 351, 352, 521, 811, 1187
AsuC2I CCSGG 1 cut(s) 815
AvaII GGWCC 2 cut(s) 811, 1187
BaeGI GKGCMC 3 cut(s) 281, 355, 932
BanI GGYRCC 1 cut(s) 278
BanII GRGCYC 1 cut(s) 355
BbsI GAAGAC 1 cut(s) 617
BbvI GCAGC 1 cut(s) 383
BccI CCATC 3 cut(s) 282, 628, 1198
BcnI CCSGG 1 cut(s) 815
BfaI CTAG 2 cut(s) 71, 992
BfoI RGCGCY 2 cut(s) 397, 775
BisI GCNGC 2 cut(s) 397, 1321
BlsI GCNGC 2 cut(s) 398, 1322
BmcAI AGTACT 1 cut(s) 1090
Bme1390I CCNGG 1 cut(s) 815
Bme18I GGWCC 2 cut(s) 811, 1187
BmgT120I GGNCC 5 cut(s) 351, 352, 521, 811, 1187
BmiI GGNNCC 4 cut(s) 88, 280, 353, 813
BmrFI CCNGG 1 cut(s) 815
BmrI ACTGGG 1 cut(s) 528
BmsI GCATC 1 cut(s) 1294
BmuI ACTGGG 1 cut(s) 528
BpiI GAAGAC 1 cut(s) 617
BplI GAGNNNNNCTC 2 cut(s) 1221, 1253
BpuEI CTTGAG 1 cut(s) 552
BpuMI CCSGG 1 cut(s) 815
BsaJI CCNNGG 1 cut(s) 827
BsaWI WCCGGW 2 cut(s) 780, 1042
Bsc4I CCNNNNNNNGG 5 cut(s) 45, 243, 288, 926, 1246
Bse1I ACTGG 2 cut(s) 523, 878
Bse3DI GCAATG 1 cut(s) 591
BseAI TCCGGA 1 cut(s) 1042
BseDI CCNNGG 1 cut(s) 827
BseGI GGATG 6 cut(s) 39, 327, 565, 700, 715, 1296
BseLI CCNNNNNNNGG 5 cut(s) 45, 243, 288, 926, 1246
BseMI GCAATG 1 cut(s) 591
BseNI ACTGG 2 cut(s) 523, 878
BseSI GKGCMC 3 cut(s) 281, 355, 932
BseXI GCAGC 1 cut(s) 383
BseYI CCCAGC 2 cut(s) 252, 612
BshFI GGCC 2 cut(s) 353, 523
BshNI GGYRCC 1 cut(s) 278
BsiSI CCGG 3 cut(s) 781, 815, 1043
BslFI GGGAC 2 cut(s) 797, 1291
BslI CCNNNNNNNGG 5 cut(s) 45, 243, 288, 926, 1246
BsmFI GGGAC 2 cut(s) 797, 1291
BsmI GAATGC 1 cut(s) 93
BsnI GGCC 2 cut(s) 353, 523
Bsp120I GGGCCC 1 cut(s) 351
Bsp1286I GDGCHC 3 cut(s) 281, 355, 932
Bsp13I TCCGGA 1 cut(s) 1042
Bsp1407I TGTACA 1 cut(s) 912
Bsp143I GATC 2 cut(s) 988, 1143
BspACI CCGC 2 cut(s) 319, 1124
BspANI GGCC 2 cut(s) 353, 523
BspEI TCCGGA 1 cut(s) 1042
BspLI GGNNCC 4 cut(s) 88, 280, 353, 813
BspT107I GGYRCC 1 cut(s) 278
BsrDI GCAATG 1 cut(s) 591
BsrGI TGTACA 1 cut(s) 912
BsrI ACTGG 2 cut(s) 523, 878
BssECI CCNNGG 1 cut(s) 827
BssMI GATC 2 cut(s) 988, 1143
Bst4CI ACNGT 1 cut(s) 555
BstAPI GCANNNNNTGC 1 cut(s) 1070
BstAUI TGTACA 1 cut(s) 912
BstC8I GCNNGC 4 cut(s) 91, 879, 1252, 1256
BstDEI CTNAG 1 cut(s) 1118
BstF5I GGATG 6 cut(s) 39, 327, 565, 700, 715, 1296
BstH2I RGCGCY 2 cut(s) 397, 775
BstHHI GCGC 3 cut(s) 396, 612, 774
BstKTI GATC 2 cut(s) 991, 1146
BstMBI GATC 2 cut(s) 988, 1143
BstMWI GCNNNNNNNGC 1 cut(s) 1070
BstNSI RCATGY 3 cut(s) 688, 881, 1258
BstSCI CCNGG 1 cut(s) 813
BstSLI GKGCMC 3 cut(s) 281, 355, 932
BstV1I GCAGC 1 cut(s) 383
BstV2I GAAGAC 1 cut(s) 617
BsuRI GGCC 2 cut(s) 353, 523
BtsCI GGATG 6 cut(s) 39, 327, 565, 700, 715, 1296
BtsIMutI CAGTG 2 cut(s) 685, 791
Cac8I GCNNGC 4 cut(s) 91, 879, 1252, 1256
CfoI GCGC 3 cut(s) 396, 612, 774
Cfr13I GGNCC 5 cut(s) 351, 352, 521, 811, 1187
Csp6I GTAC 3 cut(s) 481, 913, 1089
CviQI GTAC 3 cut(s) 481, 913, 1089
DdeI CTNAG 1 cut(s) 1118
DpnI GATC 2 cut(s) 990, 1145
DpnII GATC 2 cut(s) 988, 1143
DraI TTTAAA 1 cut(s) 21
Eco24I GRGCYC 1 cut(s) 355
Eco47I GGWCC 2 cut(s) 811, 1187
Eco47III AGCGCT 1 cut(s) 395
EcoO109I RGGNCCY 1 cut(s) 811
EcoT22I ATGCAT 2 cut(s) 544, 1075
EcoT38I GRGCYC 1 cut(s) 355
FalI AAGNNNNNCTT 2 cut(s) 455, 487
FaqI GGGAC 2 cut(s) 797, 1291
FauNDI CATATG 1 cut(s) 767
Fnu4HI GCNGC 2 cut(s) 397, 1321
FokI GGATG 6 cut(s) 46, 334, 572, 687, 722, 1283
FriOI GRGCYC 1 cut(s) 355
Fsp4HI GCNGC 2 cut(s) 397, 1321
FspBI CTAG 2 cut(s) 71, 992
GlaI GCGC 3 cut(s) 395, 611, 773
GluI GCNGC 2 cut(s) 397, 1321
GsaI CCCAGC 2 cut(s) 256, 616
HaeII RGCGCY 2 cut(s) 397, 775
HaeIII GGCC 2 cut(s) 353, 523
HapII CCGG 3 cut(s) 781, 815, 1043
HhaI GCGC 3 cut(s) 396, 612, 774
Hin6I GCGC 3 cut(s) 394, 610, 772
HinP1I GCGC 3 cut(s) 394, 610, 772
HindIII AAGCTT 5 cut(s) 10, 328, 941, 1001, 1283
HinfI GANTC 2 cut(s) 362, 1236
HpaII CCGG 3 cut(s) 781, 815, 1043
Hpy166II GTNNAC 1 cut(s) 1115
Hpy188I TCNGA 2 cut(s) 633, 936
Hpy188III TCNNGA 7 cut(s) 454, 569, 992, 1043, 1084, 1112, 1141
Hpy8I GTNNAC 1 cut(s) 1115
Hpy99I CGWCG 1 cut(s) 481
HpyAV CCTTC 2 cut(s) 215, 1226
HpyCH4III ACNGT 1 cut(s) 555
HpyCH4IV ACGT 1 cut(s) 479
HpyF10VI GCNNNNNNNGC 1 cut(s) 1070
HpyF3I CTNAG 1 cut(s) 1118
HpySE526I ACGT 1 cut(s) 479
HspAI GCGC 3 cut(s) 394, 610, 772
Kpn2I TCCGGA 1 cut(s) 1042
Kzo9I GATC 2 cut(s) 988, 1143
LmnI GCTCC 1 cut(s) 86
Lsp1109I GCAGC 1 cut(s) 383
LweI GCATC 1 cut(s) 1294
MaeI CTAG 2 cut(s) 71, 992
MaeII ACGT 1 cut(s) 479
MaeIII GTNAC 3 cut(s) 425, 696, 789
MalI GATC 2 cut(s) 990, 1145
MboI GATC 2 cut(s) 988, 1143
MboII GAAGA 4 cut(s) 191, 617, 620, 880
MfeI CAATTG 1 cut(s) 240
MhlI GDGCHC 3 cut(s) 281, 355, 932
MluCI AATT 4 cut(s) 124, 240, 841, 1217
MlyI GAGTC 1 cut(s) 1230
MmeI TCCRAC 1 cut(s) 537
Mph1103I ATGCAT 2 cut(s) 544, 1075
MroI TCCGGA 1 cut(s) 1042
MseI TTAA 4 cut(s) 20, 303, 737, 1224
MslI CAYNNNNRTG 3 cut(s) 796, 1015, 1299
MspI CCGG 3 cut(s) 781, 815, 1043
MspR9I CCNGG 1 cut(s) 815
MunI CAATTG 1 cut(s) 240
Mva1269I GAATGC 1 cut(s) 93
MwoI GCNNNNNNNGC 1 cut(s) 1070
NciI CCSGG 1 cut(s) 815
NdeI CATATG 1 cut(s) 767
NdeII GATC 2 cut(s) 988, 1143
NlaIV GGNNCC 4 cut(s) 88, 280, 353, 813
NmeAIII GCCGAG 1 cut(s) 852
NmuCI GTSAC 1 cut(s) 789
NsiI ATGCAT 2 cut(s) 544, 1075
NspI RCATGY 3 cut(s) 688, 881, 1258
OliI CACNNNNGTG 1 cut(s) 1299
PaeI GCATGC 2 cut(s) 881, 1258
PctI GAATGC 1 cut(s) 93
PfeI GAWTC 1 cut(s) 362
PflMI CCANNNNNTGG 1 cut(s) 45
PfoI TCCNGGA 1 cut(s) 813
PkrI GCNGC 2 cut(s) 398, 1322
PleI GAGTC 1 cut(s) 1230
PpsI GAGTC 1 cut(s) 1230
PpuMI RGGWCCY 1 cut(s) 811
Psp5II RGGWCCY 1 cut(s) 811
PspFI CCCAGC 2 cut(s) 252, 612
PspN4I GGNNCC 4 cut(s) 88, 280, 353, 813
PspOMI GGGCCC 1 cut(s) 351
PspPI GGNCC 5 cut(s) 351, 352, 521, 811, 1187
PspPPI RGGWCCY 1 cut(s) 811
RsaI GTAC 3 cut(s) 482, 914, 1090
RsaNI GTAC 3 cut(s) 481, 913, 1089
RseI CAYNNNNRTG 3 cut(s) 796, 1015, 1299
SaqAI TTAA 4 cut(s) 20, 303, 737, 1224
SatI GCNGC 2 cut(s) 397, 1321
Sau3AI GATC 2 cut(s) 988, 1143
Sau96I GGNCC 5 cut(s) 351, 352, 521, 811, 1187
ScaI AGTACT 1 cut(s) 1090
SchI GAGTC 1 cut(s) 1230
ScrFI CCNGG 1 cut(s) 815
SduI GDGCHC 3 cut(s) 281, 355, 932
SfaNI GCATC 1 cut(s) 1294
SinI GGWCC 2 cut(s) 811, 1187
SmiMI CAYNNNNRTG 3 cut(s) 796, 1015, 1299
SmlI CTYRAG 1 cut(s) 567
SmoI CTYRAG 1 cut(s) 567
SphI GCATGC 2 cut(s) 881, 1258
Sse9I AATT 4 cut(s) 124, 240, 841, 1217
SsiI CCGC 2 cut(s) 319, 1124
SspI AATATT 2 cut(s) 161, 403
SspMI CTAG 2 cut(s) 71, 992
StyD4I CCNGG 1 cut(s) 813
TaaI ACNGT 1 cut(s) 555
TaiI ACGT 1 cut(s) 482
TasI AATT 4 cut(s) 124, 240, 841, 1217
TatI WGTACW 2 cut(s) 912, 1088
TfiI GAWTC 1 cut(s) 362
Tru1I TTAA 4 cut(s) 20, 303, 737, 1224
Tru9I TTAA 4 cut(s) 20, 303, 737, 1224
TscAI CASTG 2 cut(s) 685, 798
TseFI GTSAC 1 cut(s) 789
TseI GCWGC 2 cut(s) 396, 1320
Tsp45I GTSAC 1 cut(s) 789
TspDTI ATGAA 6 cut(s) 5, 137, 219, 234, 733, 999
TspGWI ACGGA 1 cut(s) 1289
TspRI CASTG 2 cut(s) 685, 798
Van91I CCANNNNNTGG 1 cut(s) 45
VpaK11BI GGWCC 2 cut(s) 811, 1187
XapI RAATTY 1 cut(s) 124
XbaI TCTAGA 1 cut(s) 991
XceI RCATGY 3 cut(s) 688, 881, 1258
XspI CTAG 2 cut(s) 71, 992
ZrmI AGTACT 1 cut(s) 1090
Zsp2I ATGCAT 2 cut(s) 544, 1075
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.