pycom09g00070

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0007749
Physical Location & Seq
Forward (+)
48040 .. 49349
1310 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g00070.1

Sequence Viewer

Length: 711 bp
ATGTTGGAAAAGCTTATAGAGGAGAGAAGAGGTTCCAAAGAAGTCCATCAGGACATGCTTGGCTGCTTACTGAGAAGTGATGAAAACAAAAACAAACTAAGTGACGAAGAAATAATCGATATGGTCGTCACGATTTTGTACTCGGGTTATGAGACCGTTTCGACTACTTCCATGATGGCTGTCAAGTACCTCCATGATCATCCCAAAGTGCTTGAAGAACTCAGAAAAGAGCATTTGGCAATTAGAGAAAAGAAAAATCCAGAGGACCCAATTGACTGGAACGACTTGAAATCTATGAAGTTTACTCGTGCGGTAATATTTGAGACCTCAAGATTGGCCACCATTGTGAATGGGGTATTAAGAAAAACCACTCAAGATATGGAACTAAATGGATATTTGATTCCGAAAGGATGGAGAATCTATGTGTATACAAGAGAGATTAATTATGACTCCTTTCTGTATCCGGAACCTTTAACCTTCAACCCATGGAGATGGCTGGACAAGGGCTTGGAGTCTTCAAACTTCTTCTTTATATTTGGAGGTGGCACCAGGCTCTGTCCAGGAAGGGAGTTGGGGATAGCAGAAATTTCAACTTTCCTGCACTACTTTGTAACTAGATACAGGTGGGAAGAAGTTGGGGGAGACAAACTGATGAAATTTCCAAGAGTAGAGGCCCCGAATGGACTGCACCTTAGGGTTTCATCTTACTGA

Protein Analysis

237

Amino Acids

27.7

Weight (kDa)

6.34

Isoelectric Point (pI)

42.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 2 - 221 5.6e-49 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 545
AccI GTMKAC 1 cut(s) 428
AccIII TCCGGA 1 cut(s) 463
AciI CCGC 1 cut(s) 311
AcoI YGGCCR 1 cut(s) 336
AcsI RAATTY 2 cut(s) 585, 656
AfaI GTAC 2 cut(s) 140, 188
AgsI TTSAA 5 cut(s) 215, 289, 481, 519, 591
AjnI CCWGG 2 cut(s) 548, 559
AleI CACNNNNGTG 1 cut(s) 344
AluBI AGCT 1 cut(s) 13
AluI AGCT 1 cut(s) 13
Alw26I GTCTC 3 cut(s) 146, 317, 636
AlwNI CAGNNNCTG 1 cut(s) 555
Ama87I CYCGRG 1 cut(s) 142
Aor13HI TCCGGA 1 cut(s) 463
AoxI GGCC 2 cut(s) 336, 672
ApeKI GCWGC 1 cut(s) 63
ApoI RAATTY 2 cut(s) 585, 656
AseI ATTAAT 1 cut(s) 441
Asp700I GAANNNNTTC 1 cut(s) 31
AspS9I GGNCC 2 cut(s) 265, 673
AvaI CYCGRG 1 cut(s) 142
AvaII GGWCC 1 cut(s) 265
AxyI CCTNAGG 1 cut(s) 692
BalI TGGCCA 1 cut(s) 338
BanI GGYRCC 1 cut(s) 545
BauI CACGAG 1 cut(s) 306
BbsI GAAGAC 1 cut(s) 507
BbvI GCAGC 1 cut(s) 50
BccI CCATC 4 cut(s) 54, 169, 405, 486
BcgI CGANNNNNNTGC 2 cut(s) 667, 701
BciT130I CCWGG 2 cut(s) 550, 561
BciVI GTATCC 1 cut(s) 471
BclI TGATCA 1 cut(s) 196
BcoDI GTCTC 3 cut(s) 146, 317, 636
BfaI CTAG 1 cut(s) 615
BfuI GTATCC 1 cut(s) 471
BisI GCNGC 1 cut(s) 64
BlsI GCNGC 1 cut(s) 65
Bme1390I CCNGG 2 cut(s) 550, 561
Bme18I GGWCC 1 cut(s) 265
BmeT110I CYCGRG 1 cut(s) 142
BmgT120I GGNCC 2 cut(s) 265, 673
BmiI GGNNCC 5 cut(s) 34, 267, 468, 547, 675
BmrFI CCNGG 2 cut(s) 550, 561
BpiI GAAGAC 1 cut(s) 507
BpuEI CTTGAG 2 cut(s) 313, 357
Bsa29I ATCGAT 1 cut(s) 117
BsaI GGTCTC 2 cut(s) 146, 317
BsaJI CCNNGG 1 cut(s) 485
BsaWI WCCGGW 1 cut(s) 463
Bse1I ACTGG 1 cut(s) 281
Bse21I CCTNAGG 1 cut(s) 692
BseAI TCCGGA 1 cut(s) 463
BseBI CCWGG 2 cut(s) 550, 561
BseCI ATCGAT 1 cut(s) 117
BseDI CCNNGG 1 cut(s) 485
BseGI GGATG 2 cut(s) 199, 416
BseMII CTCAG 2 cut(s) 62, 235
BseNI ACTGG 1 cut(s) 281
BseRI GAGGAG 1 cut(s) 35
BseXI GCAGC 1 cut(s) 50
BsgI GTGCAG 2 cut(s) 584, 671
BshFI GGCC 2 cut(s) 338, 674
BshNI GGYRCC 1 cut(s) 545
BshVI ATCGAT 1 cut(s) 117
BsiHKCI CYCGRG 1 cut(s) 142
BsiSI CCGG 1 cut(s) 464
BsmAI GTCTC 3 cut(s) 146, 317, 636
BsnI GGCC 2 cut(s) 338, 674
Bso31I GGTCTC 2 cut(s) 146, 317
BsoBI CYCGRG 1 cut(s) 142
Bsp13I TCCGGA 1 cut(s) 463
Bsp143I GATC 1 cut(s) 196
Bsp19I CCATGG 1 cut(s) 485
BspACI CCGC 1 cut(s) 311
BspANI GGCC 2 cut(s) 338, 674
BspCNI CTCAG 2 cut(s) 63, 234
BspDI ATCGAT 1 cut(s) 117
BspEI TCCGGA 1 cut(s) 463
BspLI GGNNCC 5 cut(s) 34, 267, 468, 547, 675
BspT107I GGYRCC 1 cut(s) 545
BspTNI GGTCTC 2 cut(s) 146, 317
BsrI ACTGG 1 cut(s) 281
BssECI CCNNGG 1 cut(s) 485
BssMI GATC 1 cut(s) 196
BssNAI GTATAC 1 cut(s) 429
BssSI CACGAG 1 cut(s) 306
BssT1I CCWWGG 1 cut(s) 485
Bst1107I GTATAC 1 cut(s) 429
Bst2BI CACGAG 1 cut(s) 306
Bst2UI CCWGG 2 cut(s) 550, 561
Bst4CI ACNGT 1 cut(s) 157
Bst6I CTCTTC 1 cut(s) 22
BstDEI CTNAG 4 cut(s) 71, 98, 221, 692
BstDSI CCRYGG 1 cut(s) 485
BstF5I GGATG 2 cut(s) 199, 416
BstKTI GATC 1 cut(s) 199
BstMAI GTCTC 3 cut(s) 146, 317, 636
BstMBI GATC 1 cut(s) 196
BstNI CCWGG 2 cut(s) 550, 561
BstNSI RCATGY 1 cut(s) 58
BstSCI CCNGG 2 cut(s) 548, 559
BstV1I GCAGC 1 cut(s) 50
BstV2I GAAGAC 1 cut(s) 507
BstXI CCANNNNNNTGG 2 cut(s) 276, 492
BstZ17I GTATAC 1 cut(s) 429
Bsu15I ATCGAT 1 cut(s) 117
Bsu36I CCTNAGG 1 cut(s) 692
BsuI GTATCC 1 cut(s) 471
BsuRI GGCC 2 cut(s) 338, 674
BsuTUI ATCGAT 1 cut(s) 117
BtgI CCRYGG 1 cut(s) 485
BtsCI GGATG 2 cut(s) 199, 416
CaiI CAGNNNCTG 1 cut(s) 555
Cfr13I GGNCC 2 cut(s) 265, 673
ClaI ATCGAT 1 cut(s) 117
Csp6I GTAC 2 cut(s) 139, 187
CviAII CATG 4 cut(s) 55, 172, 194, 486
CviJI RGCY 8 cut(s) 13, 63, 179, 338, 496, 507, 553, 674
CviKI_1 RGCY 8 cut(s) 13, 63, 179, 338, 496, 507, 553, 674
CviQI GTAC 2 cut(s) 139, 187
DdeI CTNAG 4 cut(s) 71, 98, 221, 692
DpnI GATC 1 cut(s) 198
DpnII GATC 1 cut(s) 196
EaeI YGGCCR 1 cut(s) 336
Eam1104I CTCTTC 1 cut(s) 22
EarI CTCTTC 1 cut(s) 22
Eco130I CCWWGG 1 cut(s) 485
Eco31I GGTCTC 2 cut(s) 146, 317
Eco47I GGWCC 1 cut(s) 265
Eco81I CCTNAGG 1 cut(s) 692
Eco88I CYCGRG 1 cut(s) 142
EcoO109I RGGNCCY 2 cut(s) 265, 673
EcoRII CCWGG 2 cut(s) 548, 559
EcoT14I CCWWGG 1 cut(s) 485
ErhI CCWWGG 1 cut(s) 485
FaeI CATG 4 cut(s) 58, 175, 197, 489
FatI CATG 4 cut(s) 54, 171, 193, 485
FbaI TGATCA 1 cut(s) 196
FblI GTMKAC 1 cut(s) 428
Fnu4HI GCNGC 1 cut(s) 64
FokI GGATG 2 cut(s) 186, 423
Fsp4HI GCNGC 1 cut(s) 64
FspBI CTAG 1 cut(s) 615
GluI GCNGC 1 cut(s) 64
HaeIII GGCC 2 cut(s) 338, 674
HapII CCGG 1 cut(s) 464
Hin1II CATG 4 cut(s) 58, 175, 197, 489
HindIII AAGCTT 1 cut(s) 11
HinfI GANTC 4 cut(s) 400, 417, 449, 512
HpaII CCGG 1 cut(s) 464
Hpy166II GTNNAC 2 cut(s) 303, 429
Hpy188I TCNGA 2 cut(s) 224, 405
Hpy188III TCNNGA 6 cut(s) 50, 130, 260, 330, 374, 464
Hpy8I GTNNAC 2 cut(s) 303, 429
HpyAV CCTTC 2 cut(s) 487, 558
HpyCH4III ACNGT 1 cut(s) 157
HpyCH4V TGCA 2 cut(s) 601, 688
HpyF3I CTNAG 4 cut(s) 71, 98, 221, 692
Hsp92II CATG 4 cut(s) 58, 175, 197, 489
Kpn2I TCCGGA 1 cut(s) 463
Ksp22I TGATCA 1 cut(s) 196
Kzo9I GATC 1 cut(s) 196
Lsp1109I GCAGC 1 cut(s) 50
MaeI CTAG 1 cut(s) 615
MaeIII GTNAC 3 cut(s) 101, 127, 610
MalI GATC 1 cut(s) 198
MboI GATC 1 cut(s) 196
MboII GAAGA 6 cut(s) 39, 119, 227, 507, 517, 641
MfeI CAATTG 1 cut(s) 270
MlsI TGGCCA 1 cut(s) 338
MluCI AATT 5 cut(s) 240, 270, 442, 585, 656
MluNI TGGCCA 1 cut(s) 338
MlyI GAGTC 2 cut(s) 443, 521
MnlI CCTC 7 cut(s) 13, 23, 200, 256, 337, 533, 664
Mox20I TGGCCA 1 cut(s) 338
MroI TCCGGA 1 cut(s) 463
MroXI GAANNNNTTC 1 cut(s) 31
MscI TGGCCA 1 cut(s) 338
MseI TTAA 3 cut(s) 359, 441, 473
MslI CAYNNNNRTG 2 cut(s) 344, 490
Msp20I TGGCCA 1 cut(s) 338
MspI CCGG 1 cut(s) 464
MspR9I CCNGG 2 cut(s) 550, 561
MunI CAATTG 1 cut(s) 270
MvaI CCWGG 2 cut(s) 550, 561
NcoI CCATGG 1 cut(s) 485
NdeII GATC 1 cut(s) 196
NlaIII CATG 4 cut(s) 58, 175, 197, 489
NlaIV GGNNCC 5 cut(s) 34, 267, 468, 547, 675
NmuCI GTSAC 2 cut(s) 101, 127
NspI RCATGY 1 cut(s) 58
OliI CACNNNNGTG 1 cut(s) 344
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PdmI GAANNNNTTC 1 cut(s) 31
PfeI GAWTC 2 cut(s) 400, 417
PfoI TCCNGGA 1 cut(s) 559
PkrI GCNGC 1 cut(s) 65
PleI GAGTC 2 cut(s) 443, 520
PpsI GAGTC 2 cut(s) 443, 520
PpuMI RGGWCCY 1 cut(s) 265
PshBI ATTAAT 1 cut(s) 441
Psp5II RGGWCCY 1 cut(s) 265
Psp6I CCWGG 2 cut(s) 548, 559
PspGI CCWGG 2 cut(s) 548, 559
PspN4I GGNNCC 5 cut(s) 34, 267, 468, 547, 675
PspPI GGNCC 2 cut(s) 265, 673
PspPPI RGGWCCY 1 cut(s) 265
PstNI CAGNNNCTG 1 cut(s) 555
RsaI GTAC 2 cut(s) 140, 188
RsaNI GTAC 2 cut(s) 139, 187
RseI CAYNNNNRTG 2 cut(s) 344, 490
SaqAI TTAA 3 cut(s) 359, 441, 473
SatI GCNGC 1 cut(s) 64
Sau3AI GATC 1 cut(s) 196
Sau96I GGNCC 2 cut(s) 265, 673
SchI GAGTC 2 cut(s) 443, 521
ScrFI CCNGG 2 cut(s) 550, 561
SetI ASST 9 cut(s) 15, 34, 192, 329, 472, 479, 544, 626, 693
SinI GGWCC 1 cut(s) 265
SmiMI CAYNNNNRTG 2 cut(s) 344, 490
SmlI CTYRAG 2 cut(s) 328, 372
SmoI CTYRAG 2 cut(s) 328, 372
Sse9I AATT 5 cut(s) 240, 270, 442, 585, 656
SsiI CCGC 1 cut(s) 311
SspI AATATT 1 cut(s) 318
SspMI CTAG 1 cut(s) 615
StyD4I CCNGG 2 cut(s) 548, 559
StyI CCWWGG 1 cut(s) 485
TaaI ACNGT 1 cut(s) 157
TaqI TCGA 2 cut(s) 117, 161
TasI AATT 5 cut(s) 240, 270, 442, 585, 656
TatI WGTACW 1 cut(s) 138
TfiI GAWTC 2 cut(s) 400, 417
Tru1I TTAA 3 cut(s) 359, 441, 473
Tru9I TTAA 3 cut(s) 359, 441, 473
TseFI GTSAC 2 cut(s) 101, 127
TseI GCWGC 1 cut(s) 63
Tsp45I GTSAC 2 cut(s) 101, 127
TspDTI ATGAA 4 cut(s) 96, 311, 668, 690
VpaK11BI GGWCC 1 cut(s) 265
VspI ATTAAT 1 cut(s) 441
XapI RAATTY 2 cut(s) 585, 656
XceI RCATGY 1 cut(s) 58
XcmI CCANNNNNNNNNTGG 1 cut(s) 376
XmiI GTMKAC 1 cut(s) 428
XmnI GAANNNNTTC 1 cut(s) 31
XspI CTAG 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.