pycom09g06070

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0015164
Physical Location & Seq
Forward (+)
4490265 .. 4491020
756 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g06070.1

Sequence Viewer

Length: 756 bp
ATGAGTAGTAGTACCGGAACCATGTCCAATAACCCTAATGATCAGTGGTTCCAATTCTACACCCAAAACCTCAATTCTTCTACTCCTGCTTCAATGTTTTGCCAGGATCAAGTCTCTGATGCCACCGACGTCACCACGACCACTACAACTTCATCTTTACCGGGTCATGCCGGTTTAGGTCACCCGAGCTCCAGCACCAACAACAGCTTGAGCCCGGATCAAGGCCGAGTTTCGAAGCCGGCTCGGAAAAGGTCTAGAGCCTCAAGAAGAACTCCCACCACGCTACTCAACACGGACACTACCAACTTTCGTGCCATGGTGCAGCAATTCACTGGGGGTCCTACTACGGCGTCGTTTCCCACTGGTGCGTCGAATCTTAGCTTTGGCCTCGGCCCCACCAGGCCACAGAACTTTGTTGTGCCTCCCGGGGCGGCGGGTTACTATCCTCTACAGCCTCAGCAGCAACAACTCTTGCAACAACAACAGCAACAATACCAACAACGTCAGAGCCAGCAATACTTGTTTTCGCTGAACAACAACAACGACAACGTTAACGGTGGAGATCATCGATTTCTTGAGAGATTGTCATCAAGAGTGGGGCCCACAAACATGGTGATGGGACTGTCGTCCTTGAGATTGCAAGTCATCGATCATCATGAGTTTCTGAGGGAGGGTGCTGCATCCCAAGTGCCTTCTTCTGCTAGGCCGCCCAGTTCTAATACTTCTGCCAATGAGAACAGGAGTTACATGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VQ

Protein Analysis

252

Amino Acids

27.47

Weight (kDa)

9.79

Isoelectric Point (pI)

56.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 91 - 116 1e-08 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015164)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22160 AT4G15120
fragaria_vesca FvH4_6g39500
prunus_persica Prupe.3G185800_v2.0.a1
pyrus_communis pycom09g06070 pycom17g12150
rosa_chinensis RchiOBHm_Chr2g0153531
rosa_laevigata RLG00000020707
rosa_multiflora Rmu_sc0003976.1_g000009
rosa_roxburghii Rroxscaffold_2G00094950
rosa_rugosa Rorug02G0439000
rosa_samantha Rh2AG503000 Rh2BG513700 Rh2CG488800 Rh2DG525400
rosa_wichuraiana Rw2G041290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 132
AciI CCGC 3 cut(s) 431, 434, 707
AclI AACGTT 1 cut(s) 549
AclWI GGATC 2 cut(s) 114, 225
AcyI GRCGYC 2 cut(s) 129, 350
AfaI GTAC 1 cut(s) 13
AfiI CCNNNNNNNGG 1 cut(s) 221
AflIII ACRYGT 1 cut(s) 747
AgsI TTSAA 1 cut(s) 93
AjnI CCWGG 2 cut(s) 102, 398
AluBI AGCT 3 cut(s) 189, 207, 381
AluI AGCT 3 cut(s) 189, 207, 381
Alw21I GWGCWC 1 cut(s) 191
Alw26I GTCTC 1 cut(s) 118
AlwI GGATC 2 cut(s) 114, 225
Ama87I CYCGRG 2 cut(s) 184, 425
AoxI GGCC 6 cut(s) 223, 385, 391, 401, 599, 704
ApaI GGGCCC 1 cut(s) 603
ApeKI GCWGC 3 cut(s) 322, 460, 677
AspS9I GGNCC 4 cut(s) 338, 392, 599, 600
AsuC2I CCSGG 4 cut(s) 162, 215, 426, 427
AsuHPI GGTGA 3 cut(s) 124, 173, 625
AsuII TTCGAA 1 cut(s) 233
AvaI CYCGRG 2 cut(s) 184, 425
AvaII GGWCC 1 cut(s) 338
BaeGI GKGCMC 1 cut(s) 603
BanII GRGCYC 3 cut(s) 191, 215, 603
Bbv12I GWGCWC 1 cut(s) 191
BbvCI CCTCAGC 1 cut(s) 456
BbvI GCAGC 3 cut(s) 334, 472, 664
BccI CCATC 1 cut(s) 610
BceAI ACGGC 1 cut(s) 363
BciT130I CCWGG 2 cut(s) 104, 400
BclI TGATCA 1 cut(s) 40
BcnI CCSGG 4 cut(s) 162, 215, 426, 427
BcoDI GTCTC 1 cut(s) 118
BfaI CTAG 2 cut(s) 255, 702
BfmI CTRYAG 1 cut(s) 449
BisI GCNGC 5 cut(s) 323, 432, 461, 678, 707
BlsI GCNGC 5 cut(s) 324, 433, 462, 679, 708
Bme1390I CCNGG 6 cut(s) 104, 162, 215, 400, 426, 427
Bme18I GGWCC 1 cut(s) 338
BmeT110I CYCGRG 2 cut(s) 184, 425
BmgT120I GGNCC 4 cut(s) 338, 392, 599, 600
BmiI GGNNCC 6 cut(s) 19, 50, 339, 394, 600, 601
BmrFI CCNGG 6 cut(s) 104, 162, 215, 400, 426, 427
BmrI ACTGGG 2 cut(s) 342, 705
BmsI GCATC 2 cut(s) 109, 689
BmuI ACTGGG 2 cut(s) 342, 705
BoxI GACNNNNGTC 1 cut(s) 625
BpmI CTGGAG 1 cut(s) 175
Bpu10I CCTNAGC 1 cut(s) 456
Bpu14I TTCGAA 1 cut(s) 233
BpuEI CTTGAG 4 cut(s) 229, 247, 596, 652
BpuMI CCSGG 4 cut(s) 162, 215, 426, 427
Bsa29I ATCGAT 2 cut(s) 568, 648
BsaBI GATNNNNATC 1 cut(s) 586
BsaHI GRCGYC 2 cut(s) 129, 350
BsaJI CCNNGG 4 cut(s) 315, 388, 425, 426
BsaWI WCCGGW 1 cut(s) 14
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bsc4I CCNNNNNNNGG 1 cut(s) 221
Bse118I RCCGGY 2 cut(s) 170, 238
Bse1I ACTGG 3 cut(s) 337, 367, 711
Bse8I GATNNNNATC 1 cut(s) 586
BseBI CCWGG 2 cut(s) 104, 400
BseCI ATCGAT 2 cut(s) 568, 648
BseDI CCNNGG 4 cut(s) 315, 388, 425, 426
BseGI GGATG 1 cut(s) 680
BseJI GATNNNNATC 1 cut(s) 586
BseLI CCNNNNNNNGG 1 cut(s) 221
BseMII CTCAG 2 cut(s) 470, 656
BseNI ACTGG 3 cut(s) 337, 367, 711
BseSI GKGCMC 1 cut(s) 603
BseXI GCAGC 3 cut(s) 334, 472, 664
BsgI GTGCAG 1 cut(s) 341
BshFI GGCC 6 cut(s) 225, 387, 393, 403, 601, 706
BshVI ATCGAT 2 cut(s) 568, 648
BsiHKAI GWGCWC 1 cut(s) 191
BsiHKCI CYCGRG 2 cut(s) 184, 425
BsiSI CCGG 6 cut(s) 15, 161, 171, 215, 239, 426
BslFI GGGAC 1 cut(s) 633
BslI CCNNNNNNNGG 1 cut(s) 221
BsmAI GTCTC 1 cut(s) 118
BsmFI GGGAC 1 cut(s) 633
BsnI GGCC 6 cut(s) 225, 387, 393, 403, 601, 706
BsoBI CYCGRG 2 cut(s) 184, 425
Bsp119I TTCGAA 1 cut(s) 233
Bsp120I GGGCCC 1 cut(s) 599
Bsp1286I GDGCHC 3 cut(s) 191, 215, 603
Bsp143I GATC 5 cut(s) 40, 106, 217, 562, 649
Bsp19I CCATGG 1 cut(s) 315
BspACI CCGC 3 cut(s) 431, 434, 707
BspANI GGCC 6 cut(s) 225, 387, 393, 403, 601, 706
BspCNI CTCAG 2 cut(s) 469, 657
BspDI ATCGAT 2 cut(s) 568, 648
BspHI TCATGA 1 cut(s) 655
BspLI GGNNCC 6 cut(s) 19, 50, 339, 394, 600, 601
BspPI GGATC 2 cut(s) 114, 225
BspT104I TTCGAA 1 cut(s) 233
BsrFI RCCGGY 2 cut(s) 170, 238
BsrI ACTGG 3 cut(s) 337, 367, 711
BssAI RCCGGY 2 cut(s) 170, 238
BssECI CCNNGG 4 cut(s) 315, 388, 425, 426
BssMI GATC 5 cut(s) 40, 106, 217, 562, 649
BssNI GRCGYC 2 cut(s) 129, 350
BssT1I CCWWGG 1 cut(s) 315
Bst2UI CCWGG 2 cut(s) 104, 400
Bst4CI ACNGT 2 cut(s) 557, 624
BstACI GRCGYC 2 cut(s) 129, 350
BstBI TTCGAA 1 cut(s) 233
BstC8I GCNNGC 2 cut(s) 240, 512
BstDEI CTNAG 3 cut(s) 377, 456, 665
BstDSI CCRYGG 1 cut(s) 315
BstEII GGTNACC 1 cut(s) 179
BstF5I GGATG 1 cut(s) 680
BstKTI GATC 5 cut(s) 43, 109, 220, 565, 652
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 5 cut(s) 40, 106, 217, 562, 649
BstMWI GCNNNNNNNGC 1 cut(s) 460
BstNI CCWGG 2 cut(s) 104, 400
BstNSI RCATGY 1 cut(s) 751
BstPAI GACNNNNGTC 1 cut(s) 625
BstPI GGTNACC 1 cut(s) 179
BstSCI CCNGG 6 cut(s) 102, 160, 213, 398, 424, 425
BstSFI CTRYAG 1 cut(s) 449
BstSLI GKGCMC 1 cut(s) 603
BstV1I GCAGC 3 cut(s) 334, 472, 664
BstXI CCANNNNNNTGG 1 cut(s) 610
Bsu15I ATCGAT 2 cut(s) 568, 648
BsuRI GGCC 6 cut(s) 225, 387, 393, 403, 601, 706
BsuTUI ATCGAT 2 cut(s) 568, 648
BtgI CCRYGG 1 cut(s) 315
BtsCI GGATG 1 cut(s) 680
BtsIMutI CAGTG 3 cut(s) 50, 330, 360
Cac8I GCNNGC 2 cut(s) 240, 512
CciI TCATGA 1 cut(s) 655
Cfr10I RCCGGY 2 cut(s) 170, 238
Cfr13I GGNCC 4 cut(s) 338, 392, 599, 600
Cfr9I CCCGGG 1 cut(s) 425
ClaI ATCGAT 2 cut(s) 568, 648
CseI GACGC 2 cut(s) 339, 357
Csp6I GTAC 1 cut(s) 12
CviAII CATG 6 cut(s) 22, 167, 316, 610, 656, 748
CviQI GTAC 1 cut(s) 12
DdeI CTNAG 3 cut(s) 377, 456, 665
DpnI GATC 5 cut(s) 42, 108, 219, 564, 651
DpnII GATC 5 cut(s) 40, 106, 217, 562, 649
Ecl136II GAGCTC 1 cut(s) 189
Eco130I CCWWGG 1 cut(s) 315
Eco24I GRGCYC 3 cut(s) 191, 215, 603
Eco47I GGWCC 1 cut(s) 338
Eco53kI GAGCTC 1 cut(s) 189
Eco88I CYCGRG 2 cut(s) 184, 425
Eco91I GGTNACC 1 cut(s) 179
EcoICRI GAGCTC 1 cut(s) 189
EcoO109I RGGNCCY 2 cut(s) 338, 599
EcoO65I GGTNACC 1 cut(s) 179
EcoRII CCWGG 2 cut(s) 102, 398
EcoT14I CCWWGG 1 cut(s) 315
EcoT38I GRGCYC 3 cut(s) 191, 215, 603
ErhI CCWWGG 1 cut(s) 315
FaeI CATG 6 cut(s) 25, 170, 319, 613, 659, 751
FaiI YATR 6 cut(s) 23, 168, 317, 611, 657, 749
FaqI GGGAC 1 cut(s) 633
FatI CATG 6 cut(s) 21, 166, 315, 609, 655, 747
FauI CCCGC 1 cut(s) 427
FbaI TGATCA 1 cut(s) 40
Fnu4HI GCNGC 5 cut(s) 323, 432, 461, 678, 707
FokI GGATG 1 cut(s) 667
FriOI GRGCYC 3 cut(s) 191, 215, 603
Fsp4HI GCNGC 5 cut(s) 323, 432, 461, 678, 707
FspBI CTAG 2 cut(s) 255, 702
GluI GCNGC 5 cut(s) 323, 432, 461, 678, 707
GsuI CTGGAG 1 cut(s) 175
HaeIII GGCC 6 cut(s) 225, 387, 393, 403, 601, 706
HapII CCGG 6 cut(s) 15, 161, 171, 215, 239, 426
HgaI GACGC 2 cut(s) 339, 357
Hin1I GRCGYC 2 cut(s) 129, 350
Hin1II CATG 6 cut(s) 25, 170, 319, 613, 659, 751
HincII GTYRAC 1 cut(s) 553
HindII GTYRAC 1 cut(s) 553
HinfI GANTC 1 cut(s) 373
HpaI GTTAAC 1 cut(s) 553
HpaII CCGG 6 cut(s) 15, 161, 171, 215, 239, 426
HphI GGTGA 3 cut(s) 124, 173, 625
Hpy166II GTNNAC 1 cut(s) 553
Hpy188I TCNGA 4 cut(s) 118, 246, 507, 666
Hpy188III TCNNGA 5 cut(s) 255, 264, 575, 591, 656
Hpy8I GTNNAC 1 cut(s) 553
Hpy99I CGWCG 3 cut(s) 131, 355, 373
HpyAV CCTTC 1 cut(s) 702
HpyCH4III ACNGT 2 cut(s) 557, 624
HpyCH4IV ACGT 3 cut(s) 129, 502, 549
HpyCH4V TGCA 4 cut(s) 322, 475, 640, 680
HpyF10VI GCNNNNNNNGC 1 cut(s) 460
HpyF3I CTNAG 3 cut(s) 377, 456, 665
HpySE526I ACGT 3 cut(s) 129, 502, 549
Hsp92I GRCGYC 2 cut(s) 129, 350
Hsp92II CATG 6 cut(s) 25, 170, 319, 613, 659, 751
KroI GCCGGC 1 cut(s) 238
KroNI GCCGGC 1 cut(s) 240
Ksp22I TGATCA 1 cut(s) 40
KspAI GTTAAC 1 cut(s) 553
Kzo9I GATC 5 cut(s) 40, 106, 217, 562, 649
LmnI GCTCC 1 cut(s) 194
Lsp1109I GCAGC 3 cut(s) 334, 472, 664
LweI GCATC 2 cut(s) 109, 689
MaeI CTAG 2 cut(s) 255, 702
MaeII ACGT 3 cut(s) 129, 502, 549
MaeIII GTNAC 4 cut(s) 130, 179, 437, 743
MalI GATC 5 cut(s) 42, 108, 219, 564, 651
MboI GATC 5 cut(s) 40, 106, 217, 562, 649
MboII GAAGA 3 cut(s) 69, 279, 687
MhlI GDGCHC 3 cut(s) 191, 215, 603
MluCI AATT 3 cut(s) 53, 73, 326
MnlI CCTC 8 cut(s) 80, 271, 398, 432, 456, 465, 660, 664
MroNI GCCGGC 1 cut(s) 238
MseI TTAA 1 cut(s) 552
MslI CAYNNNNRTG 2 cut(s) 608, 614
MspI CCGG 6 cut(s) 15, 161, 171, 215, 239, 426
MspR9I CCNGG 6 cut(s) 104, 162, 215, 400, 426, 427
MvaI CCWGG 2 cut(s) 104, 400
MwoI GCNNNNNNNGC 1 cut(s) 460
NaeI GCCGGC 1 cut(s) 240
NciI CCSGG 4 cut(s) 162, 215, 426, 427
NcoI CCATGG 1 cut(s) 315
NdeII GATC 5 cut(s) 40, 106, 217, 562, 649
NgoMIV GCCGGC 1 cut(s) 238
NlaIII CATG 6 cut(s) 25, 170, 319, 613, 659, 751
NlaIV GGNNCC 6 cut(s) 19, 50, 339, 394, 600, 601
NmeAIII GCCGAG 2 cut(s) 251, 369
NmuCI GTSAC 2 cut(s) 130, 179
NspI RCATGY 1 cut(s) 751
NspV TTCGAA 1 cut(s) 233
PagI TCATGA 1 cut(s) 655
PciI ACATGT 1 cut(s) 747
PdiI GCCGGC 1 cut(s) 240
PfeI GAWTC 1 cut(s) 373
PkrI GCNGC 5 cut(s) 324, 433, 462, 679, 708
PpuMI RGGWCCY 1 cut(s) 338
PscI ACATGT 1 cut(s) 747
PshAI GACNNNNGTC 1 cut(s) 625
Psp124BI GAGCTC 1 cut(s) 191
Psp1406I AACGTT 1 cut(s) 549
Psp5II RGGWCCY 1 cut(s) 338
Psp6I CCWGG 2 cut(s) 102, 398
PspEI GGTNACC 1 cut(s) 179
PspGI CCWGG 2 cut(s) 102, 398
PspN4I GGNNCC 6 cut(s) 19, 50, 339, 394, 600, 601
PspOMI GGGCCC 1 cut(s) 599
PspPI GGNCC 4 cut(s) 338, 392, 599, 600
PspPPI RGGWCCY 1 cut(s) 338
PsrI GAACNNNNNNTAC 1 cut(s) 728
RsaI GTAC 1 cut(s) 13
RsaNI GTAC 1 cut(s) 12
RseI CAYNNNNRTG 2 cut(s) 608, 614
SacI GAGCTC 1 cut(s) 191
SaqAI TTAA 1 cut(s) 552
SatI GCNGC 5 cut(s) 323, 432, 461, 678, 707
Sau3AI GATC 5 cut(s) 40, 106, 217, 562, 649
Sau96I GGNCC 4 cut(s) 338, 392, 599, 600
ScrFI CCNGG 6 cut(s) 104, 162, 215, 400, 426, 427
SduI GDGCHC 3 cut(s) 191, 215, 603
SetI ASST 9 cut(s) 72, 132, 181, 191, 209, 254, 383, 505, 552
SfaNI GCATC 2 cut(s) 109, 689
SfcI CTRYAG 1 cut(s) 449
SfuI TTCGAA 1 cut(s) 233
SinI GGWCC 1 cut(s) 338
SmaI CCCGGG 1 cut(s) 427
SmiMI CAYNNNNRTG 2 cut(s) 608, 614
SmlI CTYRAG 4 cut(s) 208, 262, 575, 631
SmoI CTYRAG 4 cut(s) 208, 262, 575, 631
Sse9I AATT 3 cut(s) 53, 73, 326
SsiI CCGC 3 cut(s) 431, 434, 707
SspMI CTAG 2 cut(s) 255, 702
SstI GAGCTC 1 cut(s) 191
StyD4I CCNGG 6 cut(s) 102, 160, 213, 398, 424, 425
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 2 cut(s) 557, 624
TaiI ACGT 3 cut(s) 132, 505, 552
TaqI TCGA 4 cut(s) 233, 371, 568, 648
TasI AATT 3 cut(s) 53, 73, 326
TauI GCSGC 2 cut(s) 434, 709
TfiI GAWTC 1 cut(s) 373
Tru1I TTAA 1 cut(s) 552
Tru9I TTAA 1 cut(s) 552
TscAI CASTG 3 cut(s) 50, 337, 367
TseFI GTSAC 2 cut(s) 130, 179
TseI GCWGC 3 cut(s) 322, 460, 677
Tsp45I GTSAC 2 cut(s) 130, 179
TspDTI ATGAA 1 cut(s) 141
TspGWI ACGGA 1 cut(s) 308
TspMI CCCGGG 1 cut(s) 425
TspRI CASTG 3 cut(s) 50, 337, 367
VpaK11BI GGWCC 1 cut(s) 338
XbaI TCTAGA 1 cut(s) 254
XceI RCATGY 1 cut(s) 751
XmaI CCCGGG 1 cut(s) 425
XspI CTAG 2 cut(s) 255, 702
ZraI GACGTC 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.