pycom09g06850

Persulfide dioxygenase ETHE1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0012170
Physical Location & Seq
Forward (+)
5064751 .. 5068010
3260 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 936 bp
ATGCTTCGAATCGGCGTCCTCAGATTCGTCGCTCTCCCTCACTCCTCCTCCTCCTCCTCCTCCTCGTCTTCCCTTTTGTTCAATCCCATTTCTCCCAAATCCCTCCCTCCTCCTCCTGTAATCCGTACCAAGCTCGTTTCCCGTTCCCGAACCATGGCTTATACGGCGTCGCCTGGTTCTTCTTCCAACCTCCTCTTCCGGCAGCTCTTCGAGAAGGAGTCCTCCACCTACACTTACTTGCTCGCCGATGTCTCTCACCCCGACAAACCTGCTCTGCTGATTGACCCGGTGGACAAGACGGTGGATAGGGATATCTCCCTTGTCAAAGAACTGGGGTTGAAGTTGATCTACGCCATGAACACTCATGTTCATGCTGATCACGTGACTGGCACCGGCCTCATCAAGACTAAGCTTCCTGGTGTGAAATCTGTCATTTCCAAGGCGAGCAAATCAAAAGCGGACCTTTTGATTGAAGCCGGCGATAAAATATATTTTGGAGATCTGTTTTTGGAGGTTCGAGCTACTCCTGGGCATACATTAGGTTGTCTTACCTATGTTACAGGAGATGGGCCTGATCAACCCCAACCGAGGATGGCTTTCACTGGGGATGCACTACTCATACGTGGATGTGGGAGGACCGATTTTCAGGGTGGCAGTTCACATCAACTCTACAACTCAGTTCATTCACAGATTTTCACATTGCCAAAGGATACATTTATCTATCCTGCTCATGATTACAAGGGATTCACTGTTAGTACTGTGGGGGAGGAGATGCTTTATAATCCTCGGCTCACAAAAGATGAGGAGGGATTTAAAAGTATCATGGAAAATCTAAAACTCTCATATCCAAAAATGATTGACATAGCTGTACCTGCAAATATGGTTTGTGGATTGCAAGATTTGTCTGAAAGGCCTGCCGAGTCTGTGGCAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00920 map00920
Pfam Domains
Protein Families
Lactamase_B

Protein Analysis

312

Amino Acids

33.98

Weight (kDa)

8.59

Isoelectric Point (pI)

36.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lactamase_B PF00753 76 - 244 1.6e-11 Metallo-beta-lactamase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012170)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53580 AT1G53580
fragaria_vesca FvH4_6g38610
malus_domestica MD09G1149000.v1.1 MD17G1135300.v1.1
prunus_persica Prupe.3G177200_v2.0.a1
pyrus_communis pycom09g06850 pycom17g12930
rosa_chinensis RchiOBHm_Chr2g0152061
rosa_laevigata RLG00000020603
rosa_multiflora Rmu_sc0000760.1_g000022
rosa_roxburghii Rroxscaffold_2G00096020
rosa_rugosa Rorug02G0430500 Rorug02G0430500
rosa_samantha Rh2AG491300 Rh2BG503700 Rh2CG477700 Rh2DG514900
rosa_wichuraiana Rw2G040390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 780
Acc36I ACCTGC 2 cut(s) 277, 880
AccB1I GGYRCC 1 cut(s) 389
AciI CCGC 1 cut(s) 458
AcvI CACGTG 1 cut(s) 382
AcyI GRCGYC 2 cut(s) 15, 167
AfaI GTAC 3 cut(s) 127, 757, 870
AfiI CCNNNNNNNGG 2 cut(s) 154, 588
AgsI TTSAA 3 cut(s) 82, 340, 473
AjnI CCWGG 3 cut(s) 172, 415, 526
AjuI GAANNNNNNNTTGG 2 cut(s) 179, 211
AluBI AGCT 5 cut(s) 133, 205, 412, 521, 866
AluI AGCT 5 cut(s) 133, 205, 412, 521, 866
Alw26I GTCTC 1 cut(s) 256
AoxI GGCC 3 cut(s) 394, 569, 911
ApeKI GCWGC 1 cut(s) 202
AspS9I GGNCC 3 cut(s) 460, 569, 636
AsuC2I CCSGG 1 cut(s) 287
AsuHPI GGTGA 1 cut(s) 248
AsuII TTCGAA 1 cut(s) 7
AvaII GGWCC 2 cut(s) 460, 636
BanI GGYRCC 1 cut(s) 389
BarI GAAGNNNNNNTAC 2 cut(s) 332, 364
BbrPI CACGTG 1 cut(s) 382
BbsI GAAGAC 1 cut(s) 60
BbvI GCAGC 1 cut(s) 214
BccI CCATC 2 cut(s) 560, 586
BceAI ACGGC 1 cut(s) 180
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 3 cut(s) 174, 417, 528
BciVI GTATCC 1 cut(s) 703
BclI TGATCA 2 cut(s) 376, 574
BcnI CCSGG 1 cut(s) 287
BcoDI GTCTC 1 cut(s) 256
BfuAI ACCTGC 2 cut(s) 277, 880
BfuI GTATCC 1 cut(s) 703
BglII AGATCT 1 cut(s) 499
BisI GCNGC 1 cut(s) 203
BlsI GCNGC 1 cut(s) 204
BmcAI AGTACT 1 cut(s) 757
Bme1390I CCNGG 4 cut(s) 174, 287, 417, 528
Bme18I GGWCC 2 cut(s) 460, 636
BmgT120I GGNCC 3 cut(s) 460, 569, 636
BmiI GGNNCC 1 cut(s) 391
BmrFI CCNGG 4 cut(s) 174, 287, 417, 528
BmrI ACTGGG 2 cut(s) 341, 612
BmsI GCATC 2 cut(s) 598, 762
BmuI ACTGGG 2 cut(s) 341, 612
BpiI GAAGAC 1 cut(s) 60
Bpu14I TTCGAA 1 cut(s) 7
BpuMI CCSGG 1 cut(s) 287
BsaAI YACGTR 2 cut(s) 382, 623
BsaHI GRCGYC 2 cut(s) 15, 167
BsaJI CCNNGG 5 cut(s) 153, 438, 527, 587, 785
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 588
Bse118I RCCGGY 2 cut(s) 392, 476
Bse1I ACTGG 3 cut(s) 336, 391, 607
Bse3DI GCAATG 1 cut(s) 698
BseBI CCWGG 3 cut(s) 174, 417, 528
BseDI CCNNGG 5 cut(s) 153, 438, 527, 587, 785
BseGI GGATG 3 cut(s) 597, 613, 632
BseLI CCNNNNNNNGG 2 cut(s) 154, 588
BseMI GCAATG 1 cut(s) 698
BseMII CTCAG 2 cut(s) 34, 690
BseNI ACTGG 3 cut(s) 336, 391, 607
BseXI GCAGC 1 cut(s) 214
BshFI GGCC 3 cut(s) 396, 571, 913
BshNI GGYRCC 1 cut(s) 389
BsiSI CCGG 4 cut(s) 199, 287, 393, 477
BslI CCNNNNNNNGG 2 cut(s) 154, 588
BsmAI GTCTC 1 cut(s) 256
BsnI GGCC 3 cut(s) 396, 571, 913
Bsp119I TTCGAA 1 cut(s) 7
Bsp143I GATC 4 cut(s) 345, 376, 499, 574
Bsp19I CCATGG 1 cut(s) 153
BspACI CCGC 1 cut(s) 458
BspANI GGCC 3 cut(s) 396, 571, 913
BspCNI CTCAG 2 cut(s) 33, 689
BspHI TCATGA 1 cut(s) 730
BspLI GGNNCC 1 cut(s) 391
BspMI ACCTGC 2 cut(s) 277, 880
BspQI GCTCTTC 1 cut(s) 212
BspT104I TTCGAA 1 cut(s) 7
BspT107I GGYRCC 1 cut(s) 389
BsrDI GCAATG 1 cut(s) 698
BsrFI RCCGGY 2 cut(s) 392, 476
BsrI ACTGG 3 cut(s) 336, 391, 607
BssAI RCCGGY 2 cut(s) 392, 476
BssECI CCNNGG 5 cut(s) 153, 438, 527, 587, 785
BssMI GATC 4 cut(s) 345, 376, 499, 574
BssNI GRCGYC 2 cut(s) 15, 167
BssT1I CCWWGG 2 cut(s) 153, 438
Bst2UI CCWGG 3 cut(s) 174, 417, 528
Bst4CI ACNGT 3 cut(s) 301, 751, 760
Bst6I CTCTTC 2 cut(s) 200, 212
BstACI GRCGYC 2 cut(s) 15, 167
BstBAI YACGTR 2 cut(s) 382, 623
BstBI TTCGAA 1 cut(s) 7
BstC8I GCNNGC 4 cut(s) 243, 445, 478, 915
BstDEI CTNAG 3 cut(s) 20, 408, 676
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 3 cut(s) 597, 613, 632
BstKTI GATC 4 cut(s) 348, 379, 502, 577
BstMAI GTCTC 1 cut(s) 256
BstMBI GATC 4 cut(s) 345, 376, 499, 574
BstMWI GCNNNNNNNGC 2 cut(s) 164, 872
BstNI CCWGG 3 cut(s) 174, 417, 528
BstSCI CCNGG 4 cut(s) 172, 285, 415, 526
BstV1I GCAGC 1 cut(s) 214
BstV2I GAAGAC 1 cut(s) 60
BstX2I RGATCY 1 cut(s) 499
BstYI RGATCY 1 cut(s) 499
BsuI GTATCC 1 cut(s) 703
BsuRI GGCC 3 cut(s) 396, 571, 913
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 3 cut(s) 597, 613, 632
BtsIMutI CAGTG 2 cut(s) 600, 747
BveI ACCTGC 2 cut(s) 277, 880
Cac8I GCNNGC 4 cut(s) 243, 445, 478, 915
CciI TCATGA 1 cut(s) 730
Cfr10I RCCGGY 2 cut(s) 392, 476
Cfr13I GGNCC 3 cut(s) 460, 569, 636
CseI GACGC 2 cut(s) 4, 156
Csp6I GTAC 3 cut(s) 126, 756, 869
CviAII CATG 6 cut(s) 154, 355, 365, 371, 731, 823
CviQI GTAC 3 cut(s) 126, 756, 869
DdeI CTNAG 3 cut(s) 20, 408, 676
DpnI GATC 4 cut(s) 347, 378, 501, 576
DpnII GATC 4 cut(s) 345, 376, 499, 574
DraI TTTAAA 1 cut(s) 814
Eam1104I CTCTTC 2 cut(s) 200, 212
EarI CTCTTC 2 cut(s) 200, 212
Eco130I CCWWGG 2 cut(s) 153, 438
Eco147I AGGCCT 1 cut(s) 913
Eco32I GATATC 1 cut(s) 313
Eco47I GGWCC 2 cut(s) 460, 636
Eco72I CACGTG 1 cut(s) 382
EcoRII CCWGG 3 cut(s) 172, 415, 526
EcoRV GATATC 1 cut(s) 313
EcoT14I CCWWGG 2 cut(s) 153, 438
ErhI CCWWGG 2 cut(s) 153, 438
FaeI CATG 6 cut(s) 157, 358, 368, 374, 734, 826
FalI AAGNNNNNCTT 2 cut(s) 447, 479
FatI CATG 6 cut(s) 153, 354, 364, 370, 730, 822
FbaI TGATCA 2 cut(s) 376, 574
Fnu4HI GCNGC 1 cut(s) 203
FokI GGATG 3 cut(s) 604, 620, 639
Fsp4HI GCNGC 1 cut(s) 203
GluI GCNGC 1 cut(s) 203
HaeIII GGCC 3 cut(s) 396, 571, 913
HapII CCGG 4 cut(s) 199, 287, 393, 477
HgaI GACGC 2 cut(s) 4, 156
Hin1I GRCGYC 2 cut(s) 15, 167
Hin1II CATG 6 cut(s) 157, 358, 368, 374, 734, 826
HindIII AAGCTT 1 cut(s) 410
HinfI GANTC 5 cut(s) 9, 24, 218, 744, 920
HpaII CCGG 4 cut(s) 199, 287, 393, 477
HphI GGTGA 1 cut(s) 248
Hpy166II GTNNAC 2 cut(s) 292, 659
Hpy188I TCNGA 2 cut(s) 23, 907
Hpy188III TCNNGA 4 cut(s) 147, 211, 403, 731
Hpy8I GTNNAC 2 cut(s) 292, 659
Hpy99I CGWCG 2 cut(s) 32, 172
HpyAV CCTTC 1 cut(s) 208
HpyCH4III ACNGT 3 cut(s) 301, 751, 760
HpyCH4IV ACGT 2 cut(s) 381, 622
HpyCH4V TGCA 3 cut(s) 611, 875, 895
HpyF10VI GCNNNNNNNGC 2 cut(s) 164, 872
HpyF3I CTNAG 3 cut(s) 20, 408, 676
HpySE526I ACGT 2 cut(s) 381, 622
Hsp92I GRCGYC 2 cut(s) 15, 167
Hsp92II CATG 6 cut(s) 157, 358, 368, 374, 734, 826
KroI GCCGGC 1 cut(s) 476
KroNI GCCGGC 1 cut(s) 478
Ksp22I TGATCA 2 cut(s) 376, 574
Kzo9I GATC 4 cut(s) 345, 376, 499, 574
LguI GCTCTTC 1 cut(s) 212
Lsp1109I GCAGC 1 cut(s) 214
LweI GCATC 2 cut(s) 598, 762
MaeII ACGT 2 cut(s) 381, 622
MaeIII GTNAC 2 cut(s) 382, 556
MalI GATC 4 cut(s) 347, 378, 501, 576
MboI GATC 4 cut(s) 345, 376, 499, 574
MboII GAAGA 5 cut(s) 60, 171, 174, 187, 199
MflI RGATCY 1 cut(s) 499
MlyI GAGTC 2 cut(s) 227, 929
MmeI TCCRAC 1 cut(s) 210
MroNI GCCGGC 1 cut(s) 476
MseI TTAA 1 cut(s) 813
MslI CAYNNNNRTG 1 cut(s) 369
MspI CCGG 4 cut(s) 199, 287, 393, 477
MspR9I CCNGG 4 cut(s) 174, 287, 417, 528
MvaI CCWGG 3 cut(s) 174, 417, 528
MwoI GCNNNNNNNGC 2 cut(s) 164, 872
NaeI GCCGGC 1 cut(s) 478
NciI CCSGG 1 cut(s) 287
NcoI CCATGG 1 cut(s) 153
NdeII GATC 4 cut(s) 345, 376, 499, 574
NgoMIV GCCGGC 1 cut(s) 476
NlaIII CATG 6 cut(s) 157, 358, 368, 374, 734, 826
NlaIV GGNNCC 1 cut(s) 391
NmeAIII GCCGAG 1 cut(s) 766
NmuCI GTSAC 1 cut(s) 382
NspV TTCGAA 1 cut(s) 7
PagI TCATGA 1 cut(s) 730
PceI AGGCCT 1 cut(s) 913
PciSI GCTCTTC 1 cut(s) 212
PdiI GCCGGC 1 cut(s) 478
PfeI GAWTC 3 cut(s) 9, 24, 744
PkrI GCNGC 1 cut(s) 204
PleI GAGTC 2 cut(s) 226, 928
PmaCI CACGTG 1 cut(s) 382
PmlI CACGTG 1 cut(s) 382
PpsI GAGTC 2 cut(s) 226, 928
Ppu21I YACGTR 2 cut(s) 382, 623
PsiI TTATAA 1 cut(s) 780
Psp6I CCWGG 3 cut(s) 172, 415, 526
PspCI CACGTG 1 cut(s) 382
PspGI CCWGG 3 cut(s) 172, 415, 526
PspN4I GGNNCC 1 cut(s) 391
PspPI GGNCC 3 cut(s) 460, 569, 636
PsuI RGATCY 1 cut(s) 499
RsaI GTAC 3 cut(s) 127, 757, 870
RsaNI GTAC 3 cut(s) 126, 756, 869
RseI CAYNNNNRTG 1 cut(s) 369
SapI GCTCTTC 1 cut(s) 212
SaqAI TTAA 1 cut(s) 813
SatI GCNGC 1 cut(s) 203
Sau3AI GATC 4 cut(s) 345, 376, 499, 574
Sau96I GGNCC 3 cut(s) 460, 569, 636
ScaI AGTACT 1 cut(s) 757
SchI GAGTC 2 cut(s) 227, 929
ScrFI CCNGG 4 cut(s) 174, 287, 417, 528
SfaNI GCATC 2 cut(s) 598, 762
SfuI TTCGAA 1 cut(s) 7
SinI GGWCC 2 cut(s) 460, 636
SmiMI CAYNNNNRTG 1 cut(s) 369
SseBI AGGCCT 1 cut(s) 913
SsiI CCGC 1 cut(s) 458
StuI AGGCCT 1 cut(s) 913
StyD4I CCNGG 4 cut(s) 172, 285, 415, 526
StyI CCWWGG 2 cut(s) 153, 438
TaaI ACNGT 3 cut(s) 301, 751, 760
TaiI ACGT 2 cut(s) 384, 625
TaqI TCGA 3 cut(s) 7, 210, 517
TaqII GACCGA 1 cut(s) 653
TatI WGTACW 1 cut(s) 755
TfiI GAWTC 3 cut(s) 9, 24, 744
Tru1I TTAA 1 cut(s) 813
Tru9I TTAA 1 cut(s) 813
TscAI CASTG 2 cut(s) 607, 754
TseFI GTSAC 1 cut(s) 382
TseI GCWGC 1 cut(s) 202
Tsp45I GTSAC 1 cut(s) 382
TspDTI ATGAA 3 cut(s) 359, 371, 671
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 2 cut(s) 607, 754
VpaK11BI GGWCC 2 cut(s) 460, 636
ZrmI AGTACT 1 cut(s) 757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.