pycom09g09160

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0009749
Physical Location & Seq
Forward (+)
7321498 .. 7323957
2460 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1122 bp
ATGGAAAAGCAAAGTTCTGTCTCCGTCGTTTTGTTTCTTCATGCAGCACTACTGATCTCAACTTGTTTTGGAAGCAAATCCCGGCCTGCAAAAAAACAAGTTGCCTTGTTCATCTTCGGAGACTCTTACTTAGATTCTGGCAACAACAACTACATCAACACTACAACTCTCGACCAAGCAAATTTCTGGCCATATGGAGAAACCTACTTCAAGTTCCCAACTGGAAGATTCTCCGACGGACGTTTGATGTCAGACTTTATTGCTGAGTACGCTCACCTGCCATTTGTTCCACCCTTTCTTCAACCGGGATTTCGCCAATATTATGGTGGCGCGAATTTCGCATCTGCCGGAGCTGGAGCTCTGGTGGAGACTTTTCAAGGCGAGGTCATTAACCTTAAAACACAACTGAAGTATTACAAGAAGGTAGTGACCTGGTTCAGACACAAGTTAGGCGATGTTGAAGCCAAAGTGACATTTTCAAGAGGCGTCTACTTGTTCAACATTGGAACCAACGATTATATAAGCCCTTTCCTAACCAACTCTCCCATGTTAAAGTCCTACTCTCACTCACGATACGTCGAAATGGTTATCGGCAACTTGACGAGCGTCATCCAAGAAATTTATTCAGGAGGGGGTAGGAAATTTGGGTTCATCAATTTGCTGGACTCGGGTTGTCTTCCGGGGGTCAGAATAATCAAGCCTGAAAATAATGGTAGCTGCTTAAAGGAGGTTTCGTCTCTTGCCAAATTACATAACAAAGCTCTCTCCAAACTCCTCATTCAACTTGGAAACCAGTTGGAGGGTTTCAAATATTCACTCTACGACTTAAACAGCAATCTTAGACAGAGAATCAGGCATCCCTCTAAATATGGTTTTAAGGAAGGGAAAACAGCATGTTGCGGAACAGGCCAGTTTAGAGGGGTATTTAGCTGTGGAGGGAAAAGAACAGTGAAGAAGTTTGAGTTGTGTGAGGATCCAAATGAATATGTGTTCTGGGACTCTTTGCATCTCACTGAAAAGGTCTACAAGCAATTTGCTAATGAAATGTGGAGTGGCCGGCCATTGAACTCCAAGTCCACGACAAGTCTATACAGTCTCAAGGATCTCTTCCAAGACTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

374

Amino Acids

42.06

Weight (kDa)

9.31

Isoelectric Point (pI)

32.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 36 - 348 5.1e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 285
Acc36I ACCTGC 1 cut(s) 285
AccI GTMKAC 2 cut(s) 489, 1023
AccII CGCG 1 cut(s) 332
AciI CCGC 1 cut(s) 900
AclWI GGATC 3 cut(s) 968, 981, 1110
AcoI YGGCCR 3 cut(s) 188, 1054, 1058
AcsI RAATTY 4 cut(s) 181, 334, 618, 641
AcuI CTGAAG 1 cut(s) 428
AcyI GRCGYC 1 cut(s) 486
AfaI GTAC 1 cut(s) 269
AfiI CCNNNNNNNGG 1 cut(s) 799
AgsI TTSAA 9 cut(s) 211, 302, 377, 461, 480, 499, 782, 808, 1066
AjnI CCWGG 1 cut(s) 431
AloI GAACNNNNNNTCC 2 cut(s) 1058, 1090
AluBI AGCT 5 cut(s) 353, 359, 717, 761, 930
AluI AGCT 5 cut(s) 353, 359, 717, 761, 930
Alw21I GWGCWC 1 cut(s) 361
Alw26I GTCTC 5 cut(s) 25, 114, 362, 741, 1100
AlwI GGATC 3 cut(s) 968, 981, 1110
Ama87I CYCGRG 1 cut(s) 667
AoxI GGCC 5 cut(s) 83, 188, 907, 1054, 1058
ApeKI GCWGC 2 cut(s) 44, 717
ApoI RAATTY 4 cut(s) 181, 334, 618, 641
AspLEI GCGC 1 cut(s) 332
AsuC2I CCSGG 3 cut(s) 82, 306, 681
AsuHPI GGTGA 1 cut(s) 266
AvaI CYCGRG 1 cut(s) 667
BalI TGGCCA 1 cut(s) 190
BamHI GGATCC 1 cut(s) 973
BanII GRGCYC 1 cut(s) 361
BbsI GAAGAC 1 cut(s) 668
Bbv12I GWGCWC 1 cut(s) 361
BbvI GCAGC 2 cut(s) 56, 704
BciT130I CCWGG 1 cut(s) 433
BcnI CCSGG 3 cut(s) 82, 306, 681
BcoDI GTCTC 5 cut(s) 25, 114, 362, 741, 1100
BfuAI ACCTGC 1 cut(s) 285
BisI GCNGC 2 cut(s) 45, 718
BlsI GCNGC 2 cut(s) 46, 719
Bme1390I CCNGG 4 cut(s) 82, 306, 433, 681
BmeT110I CYCGRG 1 cut(s) 667
BmiI GGNNCC 2 cut(s) 508, 975
BmrFI CCNGG 4 cut(s) 82, 306, 433, 681
BmsI GCATC 3 cut(s) 350, 865, 1015
BoxI GACNNNNGTC 1 cut(s) 605
BpiI GAAGAC 1 cut(s) 668
BpmI CTGGAG 1 cut(s) 375
BpuEI CTTGAG 1 cut(s) 1082
BpuMI CCSGG 3 cut(s) 82, 306, 681
BsaHI GRCGYC 1 cut(s) 486
BsaJI CCNNGG 1 cut(s) 680
BsaXI ACNNNNNCTCC 6 cut(s) 348, 378, 526, 556, 719, 749
Bsc4I CCNNNNNNNGG 1 cut(s) 799
Bse118I RCCGGY 1 cut(s) 1056
Bse1I ACTGG 3 cut(s) 226, 793, 910
BseBI CCWGG 1 cut(s) 433
BseDI CCNNGG 1 cut(s) 680
BseGI GGATG 2 cut(s) 609, 856
BseLI CCNNNNNNNGG 1 cut(s) 799
BseMII CTCAG 1 cut(s) 255
BseNI ACTGG 3 cut(s) 226, 793, 910
BseRI GAGGAG 1 cut(s) 764
BseXI GCAGC 2 cut(s) 56, 704
Bsh1236I CGCG 1 cut(s) 332
BshFI GGCC 5 cut(s) 85, 190, 909, 1056, 1060
BsiHKAI GWGCWC 1 cut(s) 361
BsiHKCI CYCGRG 1 cut(s) 667
BsiSI CCGG 5 cut(s) 82, 305, 348, 680, 1057
BslFI GGGAC 1 cut(s) 1010
BslI CCNNNNNNNGG 1 cut(s) 799
BsmAI GTCTC 5 cut(s) 25, 114, 362, 741, 1100
BsmBI CGTCTC 1 cut(s) 741
BsmFI GGGAC 1 cut(s) 1010
BsnI GGCC 5 cut(s) 85, 190, 909, 1056, 1060
BsoBI CYCGRG 1 cut(s) 667
Bsp1286I GDGCHC 1 cut(s) 361
Bsp143I GATC 3 cut(s) 54, 973, 1102
BspACI CCGC 1 cut(s) 900
BspANI GGCC 5 cut(s) 85, 190, 909, 1056, 1060
BspCNI CTCAG 1 cut(s) 256
BspFNI CGCG 1 cut(s) 332
BspLI GGNNCC 2 cut(s) 508, 975
BspMI ACCTGC 1 cut(s) 285
BspPI GGATC 3 cut(s) 968, 981, 1110
BsrFI RCCGGY 1 cut(s) 1056
BsrI ACTGG 3 cut(s) 226, 793, 910
BssAI RCCGGY 1 cut(s) 1056
BssECI CCNNGG 1 cut(s) 680
BssMI GATC 3 cut(s) 54, 973, 1102
BssNI GRCGYC 1 cut(s) 486
Bst2UI CCWGG 1 cut(s) 433
Bst4CI ACNGT 2 cut(s) 949, 1094
Bst6I CTCTTC 1 cut(s) 1112
BstACI GRCGYC 1 cut(s) 486
BstC8I GCNNGC 2 cut(s) 87, 1058
BstDEI CTNAG 3 cut(s) 130, 264, 839
BstF5I GGATG 2 cut(s) 609, 856
BstFNI CGCG 1 cut(s) 332
BstHHI GCGC 1 cut(s) 332
BstKTI GATC 3 cut(s) 57, 976, 1105
BstMAI GTCTC 5 cut(s) 25, 114, 362, 741, 1100
BstMBI GATC 3 cut(s) 54, 973, 1102
BstMWI GCNNNNNNNGC 3 cut(s) 269, 338, 906
BstNI CCWGG 1 cut(s) 433
BstNSI RCATGY 1 cut(s) 897
BstPAI GACNNNNGTC 1 cut(s) 605
BstSCI CCNGG 4 cut(s) 80, 304, 431, 679
BstUI CGCG 1 cut(s) 332
BstV1I GCAGC 2 cut(s) 56, 704
BstV2I GAAGAC 1 cut(s) 668
BstX2I RGATCY 2 cut(s) 973, 1102
BstXI CCANNNNNNTGG 1 cut(s) 323
BstYI RGATCY 2 cut(s) 973, 1102
BsuRI GGCC 5 cut(s) 85, 190, 909, 1056, 1060
BtgZI GCGATG 1 cut(s) 468
BtsCI GGATG 2 cut(s) 609, 856
BtsIMutI CAGTG 2 cut(s) 954, 1011
BveI ACCTGC 1 cut(s) 285
Cac8I GCNNGC 2 cut(s) 87, 1058
CfoI GCGC 1 cut(s) 332
Cfr10I RCCGGY 1 cut(s) 1056
CseI GACGC 2 cut(s) 475, 595
CsiI ACCWGGT 1 cut(s) 431
Csp6I GTAC 1 cut(s) 268
CviAII CATG 3 cut(s) 41, 547, 894
CviQI GTAC 1 cut(s) 268
DdeI CTNAG 3 cut(s) 130, 264, 839
DpnI GATC 3 cut(s) 56, 975, 1104
DpnII GATC 3 cut(s) 54, 973, 1102
EaeI YGGCCR 3 cut(s) 188, 1054, 1058
Eam1104I CTCTTC 1 cut(s) 1112
EarI CTCTTC 1 cut(s) 1112
Ecl136II GAGCTC 1 cut(s) 359
Eco24I GRGCYC 1 cut(s) 361
Eco53kI GAGCTC 1 cut(s) 359
Eco57I CTGAAG 1 cut(s) 428
Eco88I CYCGRG 1 cut(s) 667
EcoICRI GAGCTC 1 cut(s) 359
EcoRII CCWGG 1 cut(s) 431
EcoT38I GRGCYC 1 cut(s) 361
Esp3I CGTCTC 1 cut(s) 741
FaeI CATG 3 cut(s) 44, 550, 897
FalI AAGNNNNNCTT 1 cut(s) 1091
FaqI GGGAC 1 cut(s) 1010
FatI CATG 3 cut(s) 40, 546, 893
FauNDI CATATG 1 cut(s) 193
FblI GTMKAC 2 cut(s) 489, 1023
Fnu4HI GCNGC 2 cut(s) 45, 718
FokI GGATG 2 cut(s) 596, 843
FriOI GRGCYC 1 cut(s) 361
FseI GGCCGGCC 1 cut(s) 1060
Fsp4HI GCNGC 2 cut(s) 45, 718
GlaI GCGC 1 cut(s) 331
GluI GCNGC 2 cut(s) 45, 718
GsuI CTGGAG 1 cut(s) 375
HaeIII GGCC 5 cut(s) 85, 190, 909, 1056, 1060
HapII CCGG 5 cut(s) 82, 305, 348, 680, 1057
HgaI GACGC 2 cut(s) 475, 595
HhaI GCGC 1 cut(s) 332
Hin1I GRCGYC 1 cut(s) 486
Hin1II CATG 3 cut(s) 44, 550, 897
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HinfI GANTC 6 cut(s) 122, 134, 228, 665, 849, 998
HpaII CCGG 5 cut(s) 82, 305, 348, 680, 1057
HphI GGTGA 1 cut(s) 266
Hpy166II GTNNAC 3 cut(s) 490, 1024, 1077
Hpy188I TCNGA 5 cut(s) 119, 235, 253, 440, 689
Hpy188III TCNNGA 4 cut(s) 170, 480, 570, 627
Hpy8I GTNNAC 3 cut(s) 490, 1024, 1077
Hpy99I CGWCG 3 cut(s) 29, 239, 581
HpyAV CCTTC 2 cut(s) 415, 875
HpyCH4III ACNGT 2 cut(s) 949, 1094
HpyCH4IV ACGT 2 cut(s) 241, 576
HpyCH4V TGCA 3 cut(s) 44, 89, 1006
HpyF10VI GCNNNNNNNGC 3 cut(s) 269, 338, 906
HpyF3I CTNAG 3 cut(s) 130, 264, 839
HpySE526I ACGT 2 cut(s) 241, 576
Hsp92I GRCGYC 1 cut(s) 486
Hsp92II CATG 3 cut(s) 44, 550, 897
HspAI GCGC 1 cut(s) 330
KroI GCCGGC 1 cut(s) 1056
KroNI GCCGGC 1 cut(s) 1058
Kzo9I GATC 3 cut(s) 54, 973, 1102
LmnI GCTCC 2 cut(s) 350, 356
Lsp1109I GCAGC 2 cut(s) 56, 704
LweI GCATC 3 cut(s) 350, 865, 1015
MabI ACCWGGT 1 cut(s) 431
MaeII ACGT 2 cut(s) 241, 576
MaeIII GTNAC 2 cut(s) 427, 469
MalI GATC 3 cut(s) 56, 975, 1104
MboI GATC 3 cut(s) 54, 973, 1102
MboII GAAGA 7 cut(s) 29, 106, 237, 290, 668, 964, 1099
MflI RGATCY 2 cut(s) 973, 1102
MhlI GDGCHC 1 cut(s) 361
MlsI TGGCCA 1 cut(s) 190
MluCI AATT 7 cut(s) 181, 334, 618, 641, 655, 746, 1031
MluNI TGGCCA 1 cut(s) 190
MlyI GAGTC 3 cut(s) 116, 659, 992
MmeI TCCRAC 2 cut(s) 258, 777
Mox20I TGGCCA 1 cut(s) 190
MroNI GCCGGC 1 cut(s) 1056
MscI TGGCCA 1 cut(s) 190
MseI TTAA 6 cut(s) 390, 396, 551, 722, 827, 876
Msp20I TGGCCA 1 cut(s) 190
MspI CCGG 5 cut(s) 82, 305, 348, 680, 1057
MspR9I CCNGG 4 cut(s) 82, 306, 433, 681
MvaI CCWGG 1 cut(s) 433
MvnI CGCG 1 cut(s) 332
MwoI GCNNNNNNNGC 3 cut(s) 269, 338, 906
NaeI GCCGGC 1 cut(s) 1058
NciI CCSGG 3 cut(s) 82, 306, 681
NdeI CATATG 1 cut(s) 193
NdeII GATC 3 cut(s) 54, 973, 1102
NgoMIV GCCGGC 1 cut(s) 1056
NlaIII CATG 3 cut(s) 44, 550, 897
NlaIV GGNNCC 2 cut(s) 508, 975
NmuCI GTSAC 2 cut(s) 427, 469
NspI RCATGY 1 cut(s) 897
PaqCI CACCTGC 1 cut(s) 285
PdiI GCCGGC 1 cut(s) 1058
PfeI GAWTC 3 cut(s) 134, 228, 849
PkrI GCNGC 2 cut(s) 46, 719
PleI GAGTC 3 cut(s) 116, 659, 992
PpsI GAGTC 3 cut(s) 116, 659, 992
PshAI GACNNNNGTC 1 cut(s) 605
Psp124BI GAGCTC 1 cut(s) 361
Psp6I CCWGG 1 cut(s) 431
PspGI CCWGG 1 cut(s) 431
PspN4I GGNNCC 2 cut(s) 508, 975
PsuI RGATCY 2 cut(s) 973, 1102
RigI GGCCGGCC 1 cut(s) 1060
RsaI GTAC 1 cut(s) 269
RsaNI GTAC 1 cut(s) 268
SacI GAGCTC 1 cut(s) 361
SaqAI TTAA 6 cut(s) 390, 396, 551, 722, 827, 876
SatI GCNGC 2 cut(s) 45, 718
Sau3AI GATC 3 cut(s) 54, 973, 1102
SchI GAGTC 3 cut(s) 116, 659, 992
ScrFI CCNGG 4 cut(s) 82, 306, 433, 681
SduI GDGCHC 1 cut(s) 361
SexAI ACCWGGT 1 cut(s) 431
SfaNI GCATC 3 cut(s) 350, 865, 1015
SmlI CTYRAG 1 cut(s) 1097
SmoI CTYRAG 1 cut(s) 1097
Sse9I AATT 7 cut(s) 181, 334, 618, 641, 655, 746, 1031
SsiI CCGC 1 cut(s) 900
SspI AATATT 2 cut(s) 320, 812
SstI GAGCTC 1 cut(s) 361
StyD4I CCNGG 4 cut(s) 80, 304, 431, 679
TaaI ACNGT 2 cut(s) 949, 1094
TaiI ACGT 2 cut(s) 244, 579
TaqI TCGA 2 cut(s) 171, 579
TasI AATT 7 cut(s) 181, 334, 618, 641, 655, 746, 1031
TfiI GAWTC 3 cut(s) 134, 228, 849
Tru1I TTAA 6 cut(s) 390, 396, 551, 722, 827, 876
Tru9I TTAA 6 cut(s) 390, 396, 551, 722, 827, 876
TscAI CASTG 2 cut(s) 954, 1018
TseFI GTSAC 2 cut(s) 427, 469
TseI GCWGC 2 cut(s) 44, 717
Tsp45I GTSAC 2 cut(s) 427, 469
TspDTI ATGAA 5 cut(s) 29, 100, 640, 996, 1056
TspGWI ACGGA 2 cut(s) 13, 252
TspRI CASTG 2 cut(s) 954, 1018
XapI RAATTY 4 cut(s) 181, 334, 618, 641
XceI RCATGY 1 cut(s) 897
XcmI CCANNNNNNNNNTGG 1 cut(s) 323
XmiI GTMKAC 2 cut(s) 489, 1023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.