pycom09g11900

Belongs to the hexokinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002903
Physical Location & Seq
Forward (+)
10113495 .. 10114300
806 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 330 bp
ATGGGGAGGAAGGCGGTGATAATCGTTACCGCAACGGCCGTTTGCGCCGTGGCGGCGGTTTTGGTGGTGCGCCACCGCATGCGGAGCTCGGGACGGTGGGCCCGGGCATCCGCCATCATCAAGGAGCTCGAGGAGAAGTGTGGCACCCCCACTGAGAAGCTGAGGCAGGTGGCTGACGCCATGGCCGTGGAGATGCATGCCGGCCTCGCTTCCGAAGGAGGCAGTAAGCTCAAGATGATCATTAGCTATGTCGACAAACTCCCCACTGGGAATGAGAAAGGGTTGTTTTATGCATTGGATCGATCGGAGGAACAAACTTCCGTGTGCTGA

Protein Analysis

110

Amino Acids

11.76

Weight (kDa)

9.15

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hexokinase_1 PF00349 40 - 105 4.6e-17 Hexokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 157
Acc36I ACCTGC 1 cut(s) 157
AccB1I GGYRCC 1 cut(s) 143
AccI GTMKAC 1 cut(s) 252
AciI CCGC 7 cut(s) 14, 30, 53, 56, 76, 82, 111
AclWI GGATC 1 cut(s) 306
AcoI YGGCCR 2 cut(s) 36, 183
AcyI GRCGYC 1 cut(s) 177
AluBI AGCT 5 cut(s) 87, 127, 160, 229, 246
AluI AGCT 5 cut(s) 87, 127, 160, 229, 246
Alw21I GWGCWC 2 cut(s) 89, 129
AlwI GGATC 1 cut(s) 306
Ama87I CYCGRG 3 cut(s) 88, 102, 128
AoxI GGCC 4 cut(s) 36, 99, 183, 202
ApaI GGGCCC 1 cut(s) 103
AspLEI GCGC 2 cut(s) 47, 72
AspS9I GGNCC 2 cut(s) 99, 100
AsuC2I CCSGG 2 cut(s) 103, 104
AsuHPI GGTGA 1 cut(s) 28
AvaI CYCGRG 3 cut(s) 88, 102, 128
BaeGI GKGCMC 1 cut(s) 103
BanI GGYRCC 1 cut(s) 143
BanII GRGCYC 3 cut(s) 89, 103, 129
Bbv12I GWGCWC 2 cut(s) 89, 129
BbvCI CCTCAGC 1 cut(s) 161
BccI CCATC 1 cut(s) 122
BceAI ACGGC 4 cut(s) 23, 32, 51, 170
BclI TGATCA 1 cut(s) 237
BcnI CCSGG 2 cut(s) 103, 104
BfuAI ACCTGC 1 cut(s) 157
BglI GCCNNNNNGGC 1 cut(s) 53
BisI GCNGC 1 cut(s) 54
BlsI GCNGC 1 cut(s) 55
Bme1390I CCNGG 2 cut(s) 103, 104
BmeT110I CYCGRG 3 cut(s) 88, 102, 128
BmgT120I GGNCC 2 cut(s) 99, 100
BmiI GGNNCC 2 cut(s) 101, 145
BmrFI CCNGG 2 cut(s) 103, 104
BmrI ACTGGG 1 cut(s) 276
BmsI GCATC 2 cut(s) 116, 183
BmuI ACTGGG 1 cut(s) 276
Bpu10I CCTNAGC 1 cut(s) 161
BpuEI CTTGAG 1 cut(s) 215
BpuMI CCSGG 2 cut(s) 103, 104
Bsa29I ATCGAT 1 cut(s) 301
BsaHI GRCGYC 1 cut(s) 177
BsaJI CCNNGG 4 cut(s) 48, 102, 180, 186
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 1 cut(s) 271
BseCI ATCGAT 1 cut(s) 301
BseDI CCNNGG 4 cut(s) 48, 102, 180, 186
BseGI GGATG 1 cut(s) 107
BseMII CTCAG 2 cut(s) 144, 152
BseNI ACTGG 1 cut(s) 271
BseRI GAGGAG 1 cut(s) 146
BseSI GKGCMC 1 cut(s) 103
BseX3I CGGCCG 1 cut(s) 36
Bsh1285I CGRYCG 2 cut(s) 39, 305
BshFI GGCC 4 cut(s) 38, 101, 185, 204
BshNI GGYRCC 1 cut(s) 143
BshVI ATCGAT 1 cut(s) 301
BsiEI CGRYCG 2 cut(s) 39, 305
BsiHKAI GWGCWC 2 cut(s) 89, 129
BsiHKCI CYCGRG 3 cut(s) 88, 102, 128
BsiSI CCGG 2 cut(s) 103, 201
BslFI GGGAC 1 cut(s) 105
BsmFI GGGAC 1 cut(s) 105
BsnI GGCC 4 cut(s) 38, 101, 185, 204
BsoBI CYCGRG 3 cut(s) 88, 102, 128
Bsp120I GGGCCC 1 cut(s) 99
Bsp1286I GDGCHC 3 cut(s) 89, 103, 129
Bsp143I GATC 3 cut(s) 237, 298, 302
Bsp19I CCATGG 1 cut(s) 180
BspACI CCGC 7 cut(s) 14, 30, 53, 56, 76, 82, 111
BspANI GGCC 4 cut(s) 38, 101, 185, 204
BspCNI CTCAG 2 cut(s) 145, 153
BspDI ATCGAT 1 cut(s) 301
BspLI GGNNCC 2 cut(s) 101, 145
BspMI ACCTGC 1 cut(s) 157
BspPI GGATC 1 cut(s) 306
BspT107I GGYRCC 1 cut(s) 143
BsrFI RCCGGY 1 cut(s) 200
BsrI ACTGG 1 cut(s) 271
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 4 cut(s) 48, 102, 180, 186
BssMI GATC 3 cut(s) 237, 298, 302
BssNI GRCGYC 1 cut(s) 177
BssT1I CCWWGG 1 cut(s) 180
Bst4CI ACNGT 1 cut(s) 96
BstACI GRCGYC 1 cut(s) 177
BstC8I GCNNGC 3 cut(s) 80, 198, 202
BstDEI CTNAG 2 cut(s) 153, 161
BstDSI CCRYGG 3 cut(s) 48, 180, 186
BstF5I GGATG 1 cut(s) 107
BstHHI GCGC 2 cut(s) 47, 72
BstKTI GATC 3 cut(s) 240, 301, 305
BstMBI GATC 3 cut(s) 237, 298, 302
BstMCI CGRYCG 2 cut(s) 39, 305
BstMWI GCNNNNNNNGC 4 cut(s) 44, 53, 84, 206
BstNSI RCATGY 2 cut(s) 82, 200
BstSCI CCNGG 2 cut(s) 101, 102
BstSLI GKGCMC 1 cut(s) 103
BstXI CCANNNNNNTGG 1 cut(s) 187
BstZI CGGCCG 1 cut(s) 36
Bsu15I ATCGAT 1 cut(s) 301
BsuRI GGCC 4 cut(s) 38, 101, 185, 204
BsuTUI ATCGAT 1 cut(s) 301
BtgI CCRYGG 3 cut(s) 48, 180, 186
BtsCI GGATG 1 cut(s) 107
BtsIMutI CAGTG 2 cut(s) 150, 264
BveI ACCTGC 1 cut(s) 157
Cac8I GCNNGC 3 cut(s) 80, 198, 202
CfoI GCGC 2 cut(s) 47, 72
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 2 cut(s) 99, 100
Cfr9I CCCGGG 1 cut(s) 102
ClaI ATCGAT 1 cut(s) 301
CseI GACGC 1 cut(s) 185
CviAII CATG 3 cut(s) 79, 181, 197
DdeI CTNAG 2 cut(s) 153, 161
DpnI GATC 3 cut(s) 239, 300, 304
DpnII GATC 3 cut(s) 237, 298, 302
EaeI YGGCCR 2 cut(s) 36, 183
EagI CGGCCG 1 cut(s) 36
EciI GGCGGA 1 cut(s) 100
Ecl136II GAGCTC 2 cut(s) 87, 127
EclXI CGGCCG 1 cut(s) 36
Eco130I CCWWGG 1 cut(s) 180
Eco24I GRGCYC 3 cut(s) 89, 103, 129
Eco52I CGGCCG 1 cut(s) 36
Eco53kI GAGCTC 2 cut(s) 87, 127
Eco88I CYCGRG 3 cut(s) 88, 102, 128
EcoICRI GAGCTC 2 cut(s) 87, 127
EcoT14I CCWWGG 1 cut(s) 180
EcoT22I ATGCAT 2 cut(s) 198, 295
EcoT38I GRGCYC 3 cut(s) 89, 103, 129
ErhI CCWWGG 1 cut(s) 180
FaeI CATG 3 cut(s) 82, 184, 200
FaiI YATR 5 cut(s) 80, 182, 198, 249, 291
FaqI GGGAC 1 cut(s) 105
FatI CATG 3 cut(s) 78, 180, 196
FbaI TGATCA 1 cut(s) 237
FblI GTMKAC 1 cut(s) 252
Fnu4HI GCNGC 1 cut(s) 54
FokI GGATG 1 cut(s) 94
FriOI GRGCYC 3 cut(s) 89, 103, 129
Fsp4HI GCNGC 1 cut(s) 54
GlaI GCGC 2 cut(s) 46, 71
GluI GCNGC 1 cut(s) 54
HaeIII GGCC 4 cut(s) 38, 101, 185, 204
HapII CCGG 2 cut(s) 103, 201
HgaI GACGC 1 cut(s) 185
HhaI GCGC 2 cut(s) 47, 72
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 3 cut(s) 82, 184, 200
Hin6I GCGC 2 cut(s) 45, 70
HinP1I GCGC 2 cut(s) 45, 70
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HpaII CCGG 2 cut(s) 103, 201
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 1 cut(s) 253
Hpy188I TCNGA 2 cut(s) 214, 307
Hpy188III TCNNGA 2 cut(s) 90, 232
Hpy8I GTNNAC 1 cut(s) 253
HpyAV CCTTC 2 cut(s) 4, 209
HpyCH4III ACNGT 1 cut(s) 96
HpyCH4V TGCA 2 cut(s) 196, 293
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 53, 84, 206
HpyF3I CTNAG 2 cut(s) 153, 161
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 3 cut(s) 82, 184, 200
HspAI GCGC 2 cut(s) 45, 70
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
Ksp22I TGATCA 1 cut(s) 237
Kzo9I GATC 3 cut(s) 237, 298, 302
LmnI GCTCC 2 cut(s) 84, 124
LpnPI CCDG 4 cut(s) 116, 152, 214, 252
LweI GCATC 2 cut(s) 116, 183
MaeIII GTNAC 1 cut(s) 25
MalI GATC 3 cut(s) 239, 300, 304
MboI GATC 3 cut(s) 237, 298, 302
MhlI GDGCHC 3 cut(s) 89, 103, 129
MnlI CCTC 5 cut(s) 124, 156, 212, 215, 301
Mph1103I ATGCAT 2 cut(s) 198, 295
MroNI GCCGGC 1 cut(s) 200
MslI CAYNNNNRTG 1 cut(s) 185
MspI CCGG 2 cut(s) 103, 201
MspR9I CCNGG 2 cut(s) 103, 104
MwoI GCNNNNNNNGC 4 cut(s) 44, 53, 84, 206
NaeI GCCGGC 1 cut(s) 202
NciI CCSGG 2 cut(s) 103, 104
NcoI CCATGG 1 cut(s) 180
NdeII GATC 3 cut(s) 237, 298, 302
NgoMIV GCCGGC 1 cut(s) 200
NlaIII CATG 3 cut(s) 82, 184, 200
NlaIV GGNNCC 2 cut(s) 101, 145
NsiI ATGCAT 2 cut(s) 198, 295
NspI RCATGY 2 cut(s) 82, 200
PaeI GCATGC 2 cut(s) 82, 200
PaeR7I CTCGAG 1 cut(s) 128
PaqCI CACCTGC 1 cut(s) 157
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PdiI GCCGGC 1 cut(s) 202
PkrI GCNGC 1 cut(s) 55
Ple19I CGATCG 1 cut(s) 305
Psp124BI GAGCTC 2 cut(s) 89, 129
PspN4I GGNNCC 2 cut(s) 101, 145
PspOMI GGGCCC 1 cut(s) 99
PspPI GGNCC 2 cut(s) 99, 100
PspXI VCTCGAGB 1 cut(s) 128
PvuI CGATCG 1 cut(s) 305
RseI CAYNNNNRTG 1 cut(s) 185
SacI GAGCTC 2 cut(s) 89, 129
SalI GTCGAC 1 cut(s) 251
SatI GCNGC 1 cut(s) 54
Sau3AI GATC 3 cut(s) 237, 298, 302
Sau96I GGNCC 2 cut(s) 99, 100
ScrFI CCNGG 2 cut(s) 103, 104
SduI GDGCHC 3 cut(s) 89, 103, 129
SetI ASST 6 cut(s) 89, 129, 162, 171, 231, 248
SfaNI GCATC 2 cut(s) 116, 183
Sfr274I CTCGAG 1 cut(s) 128
SlaI CTCGAG 1 cut(s) 128
SmaI CCCGGG 1 cut(s) 104
SmiMI CAYNNNNRTG 1 cut(s) 185
SmlI CTYRAG 2 cut(s) 128, 230
SmoI CTYRAG 2 cut(s) 128, 230
SphI GCATGC 2 cut(s) 82, 200
SrfI GCCCGGGC 1 cut(s) 104
SsiI CCGC 7 cut(s) 14, 30, 53, 56, 76, 82, 111
SstI GAGCTC 2 cut(s) 89, 129
StyD4I CCNGG 2 cut(s) 101, 102
StyI CCWWGG 1 cut(s) 180
TaaI ACNGT 1 cut(s) 96
TaqI TCGA 3 cut(s) 129, 252, 301
TauI GCSGC 1 cut(s) 56
TscAI CASTG 2 cut(s) 157, 271
TspGWI ACGGA 1 cut(s) 310
TspMI CCCGGG 1 cut(s) 102
TspRI CASTG 2 cut(s) 157, 271
XceI RCATGY 2 cut(s) 82, 200
XhoI CTCGAG 1 cut(s) 128
XmaI CCCGGG 1 cut(s) 102
XmiI GTMKAC 1 cut(s) 252
Zsp2I ATGCAT 2 cut(s) 198, 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.