pycom09g13120

NUP50 (Nucleoporin 50 kDa)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0007109
Physical Location & Seq
Forward (+)
11679349 .. 11680326
978 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g13120.1

Sequence Viewer

Length: 978 bp
ATGGCTGATTCGGGAAATGGCCTTCCACTTAATAAGAAGCGAGCTGCTGGGAGTGAACTCTCTCGGGATAACCCAGGCCTTGATGACGAGGACGATGCTTCTGAACCAGAGACAGGAACTTTCAAGAGGGCCAGTGAGGAGGTGCTGGCAACCAGAAGAATTGTTAAGGTTCGTCGCCAGCAGACTACATCGGCCCCCACTTCTAATACTTCCAATCCTTTTGCTGGTATACGCTTGGTTCCTCCAACAGAAGAAAGTCCAGCCCCTGCTGAAGCTGCAGCTAGCTCACCACATGCCTGTGAAACACGAGTTTTAGATGAGGATAATAAGCAGCCAGAAAGCAAGGTCGAACAGGCTGGTCAAGTTGGGGATGACTCTGCAGCAGATAAGGAGAACGTTGTGAAGGAGAACAATGAAATTTCCAGTGAGGCAACGGAACCTGAACCAGATGTGGGTGAAAATAAGACAAATGGGGGTGAGACAGTAAATGAGGAAGAGGAAAAGGCTGAAATTGAAGATAAGAAAGGTAATGAAGATAACAAAGATAACAAAAGCGAAAATGTAGATCCAAGCCGAGAAGGTAACTCGTTGAGCTCATTCCAACAGCTTTCGAGTAGCCAAAATGCCTTCACAAATATCTCTGGAACTGGGTTCTCCACATCCAGTTTTTCATTTGGGCTTATTTCAAAGGATGGGTCCACATTGGGTACTGGCTCTGGTTCACTTTTTGGATCAAATATTGATCAGCCTTCTGGTTTTGGTCTCTCGAGTAATGGAAGTTCTTCACTCTTTGGCACATCAGGCCTCTCAATCGTACCTAAGAGTGATGGTGCTGCCTTCCCGCAAATGCAAGAGGTTGCAGTTGAGACTGGGGAAGAAAATGAGACAGTGGTTTTCACTGCTGATGCTGCGTTGTTTGAGTTTATTGATGGAGGCTGGAAGGAACGCGGAAAGGGAGATCGATCGCCTGAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000054 GO:0000056 GO:0000082 GO:0000226 GO:0000278 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0006403 GO:0006405 GO:0006508 GO:0006511 GO:0006606 GO:0006611 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0008104 GO:0008150 GO:0008152 GO:0008536 GO:0009056 GO:0009057 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0017016 GO:0017038 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0019941 GO:0022402 GO:0022613 GO:0030163 GO:0031267 GO:0031323 GO:0031326 GO:0031503 GO:0031935 GO:0031938 GO:0033036 GO:0033043 GO:0033044 GO:0033365 GO:0033750 GO:0034504 GO:0034613 GO:0042254 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045184 GO:0046907 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051020 GO:0051128 GO:0051168 GO:0051169 GO:0051170 GO:0051171 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051603 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0060255 GO:0060968 GO:0065007 GO:0070727 GO:0071166 GO:0071426 GO:0071428 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1902275 GO:1903047 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko05166 map05166
Pfam Domains
Protein Families
NUP50 Ran_BP1

Protein Analysis

326

Amino Acids

34.2

Weight (kDa)

4.34

Isoelectric Point (pI)

59.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUP50 PF08911 12 - 77 3e-18 NUP50 (Nucleoporin 50 kDa)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 229
AccII CGCG 1 cut(s) 948
AciI CCGC 2 cut(s) 842, 948
AclI AACGTT 1 cut(s) 396
AclWI GGATC 2 cut(s) 560, 739
AcsI RAATTY 1 cut(s) 417
AcuI CTGAAG 1 cut(s) 291
AfaI GTAC 2 cut(s) 709, 816
AfiI CCNNNNNNNGG 3 cut(s) 113, 224, 452
AgsI TTSAA 3 cut(s) 124, 515, 687
AjnI CCWGG 1 cut(s) 73
AluBI AGCT 6 cut(s) 44, 275, 281, 285, 594, 607
AluI AGCT 6 cut(s) 44, 275, 281, 285, 594, 607
Alw21I GWGCWC 1 cut(s) 596
Alw26I GTCTC 5 cut(s) 104, 473, 767, 860, 878
AlwI GGATC 2 cut(s) 560, 739
AlwNI CAGNNNCTG 1 cut(s) 266
Ama87I CYCGRG 2 cut(s) 63, 766
AoxI GGCC 5 cut(s) 19, 76, 129, 192, 802
ApeKI GCWGC 7 cut(s) 44, 275, 278, 331, 380, 833, 908
ApoI RAATTY 1 cut(s) 417
ArsI GACNNNNNNTTYG 2 cut(s) 680, 712
Asp700I GAANNNNTTC 1 cut(s) 781
AspS9I GGNCC 3 cut(s) 129, 193, 696
AsuHPI GGTGA 3 cut(s) 279, 467, 488
AsuNHI GCTAGC 1 cut(s) 281
AvaI CYCGRG 2 cut(s) 63, 766
AvaII GGWCC 1 cut(s) 696
BanII GRGCYC 1 cut(s) 596
BauI CACGAG 1 cut(s) 306
Bbv12I GWGCWC 1 cut(s) 596
BbvI GCAGC 7 cut(s) 31, 262, 290, 343, 392, 820, 895
BccI CCATC 3 cut(s) 686, 821, 923
BcgI CGANNNNNNTGC 2 cut(s) 77, 111
BciT130I CCWGG 1 cut(s) 75
BclI TGATCA 1 cut(s) 742
BcoDI GTCTC 5 cut(s) 104, 473, 767, 860, 878
BfaI CTAG 1 cut(s) 282
BfmI CTRYAG 2 cut(s) 276, 378
BisI GCNGC 7 cut(s) 45, 276, 279, 332, 381, 834, 909
BlsI GCNGC 7 cut(s) 46, 277, 280, 333, 382, 835, 910
Bme1390I CCNGG 1 cut(s) 75
Bme18I GGWCC 1 cut(s) 696
BmeT110I CYCGRG 2 cut(s) 63, 766
BmgT120I GGNCC 3 cut(s) 129, 193, 696
BmiI GGNNCC 4 cut(s) 195, 240, 438, 697
BmrFI CCNGG 1 cut(s) 75
BmrI ACTGGG 2 cut(s) 657, 879
BmsI GCATC 2 cut(s) 85, 895
BmtI GCTAGC 1 cut(s) 285
BmuI ACTGGG 2 cut(s) 657, 879
Bsa29I ATCGAT 1 cut(s) 961
BsaI GGTCTC 1 cut(s) 767
BsaJI CCNNGG 1 cut(s) 73
Bsc4I CCNNNNNNNGG 3 cut(s) 113, 224, 452
Bse1I ACTGG 6 cut(s) 132, 423, 652, 663, 715, 874
BseBI CCWGG 1 cut(s) 75
BseCI ATCGAT 1 cut(s) 961
BseDI CCNNGG 1 cut(s) 73
BseGI GGATG 3 cut(s) 376, 659, 697
BseLI CCNNNNNNNGG 3 cut(s) 113, 224, 452
BseNI ACTGG 6 cut(s) 132, 423, 652, 663, 715, 874
BseRI GAGGAG 1 cut(s) 152
BseXI GCAGC 7 cut(s) 31, 262, 290, 343, 392, 820, 895
BseYI CCCAGC 1 cut(s) 47
Bsh1236I CGCG 1 cut(s) 948
Bsh1285I CGRYCG 1 cut(s) 965
BshFI GGCC 5 cut(s) 21, 78, 131, 194, 804
BshVI ATCGAT 1 cut(s) 961
BsiEI CGRYCG 1 cut(s) 965
BsiHKAI GWGCWC 1 cut(s) 596
BsiHKCI CYCGRG 2 cut(s) 63, 766
BslI CCNNNNNNNGG 3 cut(s) 113, 224, 452
BsmAI GTCTC 5 cut(s) 104, 473, 767, 860, 878
BsnI GGCC 5 cut(s) 21, 78, 131, 194, 804
Bso31I GGTCTC 1 cut(s) 767
BsoBI CYCGRG 2 cut(s) 63, 766
Bsp1286I GDGCHC 1 cut(s) 596
Bsp143I GATC 5 cut(s) 565, 731, 742, 958, 962
BspACI CCGC 2 cut(s) 842, 948
BspANI GGCC 5 cut(s) 21, 78, 131, 194, 804
BspDI ATCGAT 1 cut(s) 961
BspFNI CGCG 1 cut(s) 948
BspLI GGNNCC 4 cut(s) 195, 240, 438, 697
BspMAI CTGCAG 2 cut(s) 280, 382
BspOI GCTAGC 1 cut(s) 285
BspPI GGATC 2 cut(s) 560, 739
BspTNI GGTCTC 1 cut(s) 767
BsrI ACTGG 6 cut(s) 132, 423, 652, 663, 715, 874
BssECI CCNNGG 1 cut(s) 73
BssMI GATC 5 cut(s) 565, 731, 742, 958, 962
BssNAI GTATAC 1 cut(s) 230
BssSI CACGAG 1 cut(s) 306
Bst1107I GTATAC 1 cut(s) 230
Bst2BI CACGAG 1 cut(s) 306
Bst2UI CCWGG 1 cut(s) 75
Bst4CI ACNGT 2 cut(s) 484, 889
Bst6I CTCTTC 1 cut(s) 489
BstC8I GCNNGC 4 cut(s) 42, 147, 179, 283
BstDEI CTNAG 1 cut(s) 819
BstF5I GGATG 3 cut(s) 376, 659, 697
BstFNI CGCG 1 cut(s) 948
BstKTI GATC 5 cut(s) 568, 734, 745, 961, 965
BstMAI GTCTC 5 cut(s) 104, 473, 767, 860, 878
BstMBI GATC 5 cut(s) 565, 731, 742, 958, 962
BstMCI CGRYCG 1 cut(s) 965
BstMWI GCNNNNNNNGC 3 cut(s) 275, 801, 908
BstNI CCWGG 1 cut(s) 75
BstNSI RCATGY 1 cut(s) 296
BstSCI CCNGG 1 cut(s) 73
BstSFI CTRYAG 2 cut(s) 276, 378
BstUI CGCG 1 cut(s) 948
BstV1I GCAGC 7 cut(s) 31, 262, 290, 343, 392, 820, 895
BstX2I RGATCY 1 cut(s) 565
BstYI RGATCY 1 cut(s) 565
BstZ17I GTATAC 1 cut(s) 230
Bsu15I ATCGAT 1 cut(s) 961
BsuRI GGCC 5 cut(s) 21, 78, 131, 194, 804
BsuTUI ATCGAT 1 cut(s) 961
BtsCI GGATG 3 cut(s) 376, 659, 697
BtsI GCAGTG 1 cut(s) 897
BtsIMutI CAGTG 4 cut(s) 139, 430, 894, 897
Cac8I GCNNGC 4 cut(s) 42, 147, 179, 283
CaiI CAGNNNCTG 1 cut(s) 266
Cfr13I GGNCC 3 cut(s) 129, 193, 696
ClaI ATCGAT 1 cut(s) 961
Csp6I GTAC 2 cut(s) 708, 815
CviAII CATG 1 cut(s) 293
CviQI GTAC 2 cut(s) 708, 815
DdeI CTNAG 1 cut(s) 819
DpnI GATC 5 cut(s) 567, 733, 744, 960, 964
DpnII GATC 5 cut(s) 565, 731, 742, 958, 962
Eam1104I CTCTTC 1 cut(s) 489
EarI CTCTTC 1 cut(s) 489
Ecl136II GAGCTC 1 cut(s) 594
Eco147I AGGCCT 2 cut(s) 78, 804
Eco24I GRGCYC 1 cut(s) 596
Eco31I GGTCTC 1 cut(s) 767
Eco47I GGWCC 1 cut(s) 696
Eco53kI GAGCTC 1 cut(s) 594
Eco57I CTGAAG 1 cut(s) 291
Eco88I CYCGRG 2 cut(s) 63, 766
EcoICRI GAGCTC 1 cut(s) 594
EcoRII CCWGG 1 cut(s) 73
EcoT38I GRGCYC 1 cut(s) 596
FaeI CATG 1 cut(s) 296
FaiI YATR 2 cut(s) 230, 294
FatI CATG 1 cut(s) 292
FauI CCCGC 1 cut(s) 849
FbaI TGATCA 1 cut(s) 742
FblI GTMKAC 1 cut(s) 229
Fnu4HI GCNGC 7 cut(s) 45, 276, 279, 332, 381, 834, 909
FokI GGATG 3 cut(s) 383, 646, 704
FriOI GRGCYC 1 cut(s) 596
Fsp4HI GCNGC 7 cut(s) 45, 276, 279, 332, 381, 834, 909
FspBI CTAG 1 cut(s) 282
GluI GCNGC 7 cut(s) 45, 276, 279, 332, 381, 834, 909
GsaI CCCAGC 1 cut(s) 51
HaeIII GGCC 5 cut(s) 21, 78, 131, 194, 804
Hin1II CATG 1 cut(s) 296
HinfI GANTC 2 cut(s) 8, 374
HphI GGTGA 3 cut(s) 279, 467, 488
Hpy166II GTNNAC 4 cut(s) 56, 230, 699, 722
Hpy188I TCNGA 1 cut(s) 103
Hpy188III TCNNGA 5 cut(s) 12, 65, 124, 642, 766
Hpy8I GTNNAC 4 cut(s) 56, 230, 699, 722
Hpy99I CGWCG 1 cut(s) 177
HpyAV CCTTC 7 cut(s) 32, 397, 572, 637, 759, 847, 934
HpyCH4III ACNGT 2 cut(s) 484, 889
HpyCH4IV ACGT 1 cut(s) 396
HpyCH4V TGCA 4 cut(s) 278, 380, 850, 860
HpyF10VI GCNNNNNNNGC 3 cut(s) 275, 801, 908
HpyF3I CTNAG 1 cut(s) 819
HpySE526I ACGT 1 cut(s) 396
Hsp92II CATG 1 cut(s) 296
Ksp22I TGATCA 1 cut(s) 742
Kzo9I GATC 5 cut(s) 565, 731, 742, 958, 962
Lsp1109I GCAGC 7 cut(s) 31, 262, 290, 343, 392, 820, 895
LweI GCATC 2 cut(s) 85, 895
MaeI CTAG 1 cut(s) 282
MaeII ACGT 1 cut(s) 396
MaeIII GTNAC 1 cut(s) 581
MalI GATC 5 cut(s) 567, 733, 744, 960, 964
MboI GATC 5 cut(s) 565, 731, 742, 958, 962
MboII GAAGA 7 cut(s) 168, 263, 506, 527, 545, 774, 887
MflI RGATCY 1 cut(s) 565
MhlI GDGCHC 1 cut(s) 596
MluCI AATT 3 cut(s) 159, 417, 510
MlyI GAGTC 1 cut(s) 368
MmeI TCCRAC 2 cut(s) 269, 625
MroXI GAANNNNTTC 1 cut(s) 781
MseI TTAA 3 cut(s) 30, 165, 976
MslI CAYNNNNRTG 1 cut(s) 297
MspR9I CCNGG 1 cut(s) 75
MvaI CCWGG 1 cut(s) 75
MvnI CGCG 1 cut(s) 948
MwoI GCNNNNNNNGC 3 cut(s) 275, 801, 908
NdeII GATC 5 cut(s) 565, 731, 742, 958, 962
NheI GCTAGC 1 cut(s) 281
NlaIII CATG 1 cut(s) 296
NlaIV GGNNCC 4 cut(s) 195, 240, 438, 697
NmeAIII GCCGAG 1 cut(s) 599
NspI RCATGY 1 cut(s) 296
PaeR7I CTCGAG 1 cut(s) 766
PceI AGGCCT 2 cut(s) 78, 804
PdmI GAANNNNTTC 1 cut(s) 781
PfeI GAWTC 1 cut(s) 8
PkrI GCNGC 7 cut(s) 46, 277, 280, 333, 382, 835, 910
Ple19I CGATCG 1 cut(s) 965
PleI GAGTC 1 cut(s) 368
PpsI GAGTC 1 cut(s) 368
Psp124BI GAGCTC 1 cut(s) 596
Psp1406I AACGTT 1 cut(s) 396
Psp6I CCWGG 1 cut(s) 73
PspFI CCCAGC 1 cut(s) 47
PspGI CCWGG 1 cut(s) 73
PspN4I GGNNCC 4 cut(s) 195, 240, 438, 697
PspPI GGNCC 3 cut(s) 129, 193, 696
PsrI GAACNNNNNNTAC 2 cut(s) 763, 795
PstI CTGCAG 2 cut(s) 280, 382
PstNI CAGNNNCTG 1 cut(s) 266
PsuI RGATCY 1 cut(s) 565
PvuI CGATCG 1 cut(s) 965
RsaI GTAC 2 cut(s) 709, 816
RsaNI GTAC 2 cut(s) 708, 815
RseI CAYNNNNRTG 1 cut(s) 297
SacI GAGCTC 1 cut(s) 596
SaqAI TTAA 3 cut(s) 30, 165, 976
SatI GCNGC 7 cut(s) 45, 276, 279, 332, 381, 834, 909
Sau3AI GATC 5 cut(s) 565, 731, 742, 958, 962
Sau96I GGNCC 3 cut(s) 129, 193, 696
SchI GAGTC 1 cut(s) 368
ScrFI CCNGG 1 cut(s) 75
SduI GDGCHC 1 cut(s) 596
SfaNI GCATC 2 cut(s) 85, 895
SfcI CTRYAG 2 cut(s) 276, 378
Sfr274I CTCGAG 1 cut(s) 766
SinI GGWCC 1 cut(s) 696
SlaI CTCGAG 1 cut(s) 766
SmiMI CAYNNNNRTG 1 cut(s) 297
SmlI CTYRAG 1 cut(s) 766
SmoI CTYRAG 1 cut(s) 766
Sse9I AATT 3 cut(s) 159, 417, 510
SseBI AGGCCT 2 cut(s) 78, 804
SsiI CCGC 2 cut(s) 842, 948
SspI AATATT 1 cut(s) 739
SspMI CTAG 1 cut(s) 282
SstI GAGCTC 1 cut(s) 596
StuI AGGCCT 2 cut(s) 78, 804
StyD4I CCNGG 1 cut(s) 73
TaaI ACNGT 2 cut(s) 484, 889
TaiI ACGT 1 cut(s) 399
TaqI TCGA 4 cut(s) 348, 611, 767, 961
TasI AATT 3 cut(s) 159, 417, 510
TfiI GAWTC 1 cut(s) 8
Tru1I TTAA 3 cut(s) 30, 165, 976
Tru9I TTAA 3 cut(s) 30, 165, 976
TscAI CASTG 4 cut(s) 139, 430, 894, 904
TseI GCWGC 7 cut(s) 44, 275, 278, 331, 380, 833, 908
TspDTI ATGAA 3 cut(s) 429, 546, 660
TspGWI ACGGA 1 cut(s) 449
TspRI CASTG 4 cut(s) 139, 430, 894, 904
VpaK11BI GGWCC 1 cut(s) 696
XapI RAATTY 1 cut(s) 417
XceI RCATGY 1 cut(s) 296
XhoI CTCGAG 1 cut(s) 766
XmiI GTMKAC 1 cut(s) 229
XmnI GAANNNNTTC 1 cut(s) 781
XspI CTAG 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.