Rorug02G0343700

NUP50 (Nucleoporin 50 kDa)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007109
Physical Location & Seq
Reverse (-)
42860599 .. 42861409
811 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0343700.1

Sequence Viewer

Length: 573 bp
ATGCGCTCGCATTCCGGCGGTTTACCCAAGGAGCAGGAGCACGCTATCATTGTCTCCGCCCTCAAACAGGTAGTCCGCGGTGGCGTTAGAACAAACAATGCACCCTCCCAGGCCCACCAGCCACCGCCGCCGCAGCTGATCTATTATGCCTCATCATCATCACTTCCTCCAACGGGGGGCGCCGATCAAGTCGTGATACCGTATTCGGATTCTGACACGTGTGAGGTATGTAAGATGGAGATGGACGATTGCCTTGGCTGTGGCTTGTTCCCGCCAAGCGAGCAATACAAAGGGAAAGGGAAGAAGATGAAGACTAGCGAGTACAGGGGAGTGCGGCAGAGACCAGGGGGCAAATGGGCGGCGGAGATTCGGGACCCGCGTCGCGCTGTTCGGGTTTGGCTCGGGACGTTTCAGACGGAGGAGGAGGCAGCCAGGGCTTATGACACGGCGGCCCTCGAGTTTCGTGGAGCTGACAGAGCTAAGCTAAACTTCCCCTCATCCTCGGACACTGGTACTACCAGTAGAGCATTGACTGATGAGACCAACAGTCAAAACGAGTCCAGGAAGTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000054 GO:0000056 GO:0000082 GO:0000226 GO:0000278 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0006403 GO:0006405 GO:0006508 GO:0006511 GO:0006606 GO:0006611 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0008104 GO:0008150 GO:0008152 GO:0008536 GO:0009056 GO:0009057 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0017016 GO:0017038 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0019941 GO:0022402 GO:0022613 GO:0030163 GO:0031267 GO:0031323 GO:0031326 GO:0031503 GO:0031935 GO:0031938 GO:0033036 GO:0033043 GO:0033044 GO:0033365 GO:0033750 GO:0034504 GO:0034613 GO:0042254 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045184 GO:0046907 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051020 GO:0051128 GO:0051168 GO:0051169 GO:0051170 GO:0051171 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051603 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0060255 GO:0060968 GO:0065007 GO:0070727 GO:0071166 GO:0071426 GO:0071428 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1902275 GO:1903047 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko05166 map05166
Pfam Domains
Protein Families
NUP50 Ran_BP1

Protein Analysis

190

Amino Acids

20.78

Weight (kDa)

8.67

Isoelectric Point (pI)

48.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 107 - 156 1.5e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 179
AccII CGCG 3 cut(s) 78, 379, 384
AcvI CACGTG 1 cut(s) 219
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 2 cut(s) 323, 514
AfiI CCNNNNNNNGG 3 cut(s) 67, 173, 176
AflIII ACRYGT 2 cut(s) 216, 218
AhdI GACNNNNNGTC 1 cut(s) 546
AjnI CCWGG 4 cut(s) 108, 343, 431, 560
AluBI AGCT 4 cut(s) 136, 470, 479, 484
AluI AGCT 4 cut(s) 136, 470, 479, 484
Alw21I GWGCWC 1 cut(s) 42
Alw26I GTCTC 3 cut(s) 58, 334, 533
Ama87I CYCGRG 2 cut(s) 401, 455
AoxI GGCC 2 cut(s) 111, 450
ApeKI GCWGC 2 cut(s) 133, 428
ArsI GACNNNNNNTTYG 2 cut(s) 57, 89
AspLEI GCGC 3 cut(s) 6, 182, 386
AspS9I GGNCC 3 cut(s) 112, 373, 451
AvaI CYCGRG 2 cut(s) 401, 455
AvaII GGWCC 1 cut(s) 373
BanI GGYRCC 1 cut(s) 179
BbrPI CACGTG 1 cut(s) 219
BbsI GAAGAC 1 cut(s) 317
Bbv12I GWGCWC 1 cut(s) 42
BbvI GCAGC 2 cut(s) 145, 440
BccI CCATC 2 cut(s) 229, 235
BceAI ACGGC 1 cut(s) 462
BciT130I CCWGG 4 cut(s) 110, 345, 433, 562
BcoDI GTCTC 3 cut(s) 58, 334, 533
BfaI CTAG 1 cut(s) 315
BfoI RGCGCY 1 cut(s) 183
BisI GCNGC 7 cut(s) 128, 131, 134, 335, 360, 429, 450
BlpI GCTNAGC 1 cut(s) 480
BlsI GCNGC 7 cut(s) 129, 132, 135, 336, 361, 430, 451
Bme1390I CCNGG 4 cut(s) 110, 345, 433, 562
Bme18I GGWCC 1 cut(s) 373
BmeRI GACNNNNNGTC 1 cut(s) 546
BmeT110I CYCGRG 2 cut(s) 401, 455
BmgT120I GGNCC 3 cut(s) 112, 373, 451
BmiI GGNNCC 3 cut(s) 181, 374, 375
BmrFI CCNGG 4 cut(s) 110, 345, 433, 562
BoxI GACNNNNGTC 1 cut(s) 378
BpiI GAAGAC 1 cut(s) 317
Bpu1102I GCTNAGC 1 cut(s) 480
BsaAI YACGTR 1 cut(s) 219
BsaHI GRCGYC 1 cut(s) 180
BsaI GGTCTC 2 cut(s) 334, 533
BsaJI CCNNGG 7 cut(s) 27, 76, 108, 253, 344, 432, 501
Bsc4I CCNNNNNNNGG 3 cut(s) 67, 173, 176
Bse1I ACTGG 2 cut(s) 514, 519
BseBI CCWGG 4 cut(s) 110, 345, 433, 562
BseDI CCNNGG 7 cut(s) 27, 76, 108, 253, 344, 432, 501
BseGI GGATG 1 cut(s) 497
BseLI CCNNNNNNNGG 3 cut(s) 67, 173, 176
BseNI ACTGG 2 cut(s) 514, 519
BseRI GAGGAG 2 cut(s) 434, 437
BseXI GCAGC 2 cut(s) 145, 440
Bsh1236I CGCG 3 cut(s) 78, 379, 384
BshFI GGCC 2 cut(s) 113, 452
BshNI GGYRCC 1 cut(s) 179
BsiHKAI GWGCWC 1 cut(s) 42
BsiHKCI CYCGRG 2 cut(s) 401, 455
BsiSI CCGG 1 cut(s) 15
BslFI GGGAC 2 cut(s) 386, 418
BslI CCNNNNNNNGG 3 cut(s) 67, 173, 176
BsmAI GTCTC 3 cut(s) 58, 334, 533
BsmFI GGGAC 2 cut(s) 386, 418
BsmI GAATGC 1 cut(s) 10
BsnI GGCC 2 cut(s) 113, 452
Bso31I GGTCTC 2 cut(s) 334, 533
BsoBI CYCGRG 2 cut(s) 401, 455
Bsp1286I GDGCHC 1 cut(s) 42
Bsp143I GATC 2 cut(s) 138, 184
Bsp1720I GCTNAGC 1 cut(s) 480
BspANI GGCC 2 cut(s) 113, 452
BspFNI CGCG 3 cut(s) 78, 379, 384
BspLI GGNNCC 3 cut(s) 181, 374, 375
BspT107I GGYRCC 1 cut(s) 179
BspTNI GGTCTC 2 cut(s) 334, 533
BsrI ACTGG 2 cut(s) 514, 519
BssECI CCNNGG 7 cut(s) 27, 76, 108, 253, 344, 432, 501
BssMI GATC 2 cut(s) 138, 184
BssNI GRCGYC 1 cut(s) 180
BssT1I CCWWGG 2 cut(s) 27, 253
Bst2UI CCWGG 4 cut(s) 110, 345, 433, 562
Bst4CI ACNGT 2 cut(s) 201, 548
BstACI GRCGYC 1 cut(s) 180
BstBAI YACGTR 1 cut(s) 219
BstC8I GCNNGC 3 cut(s) 8, 42, 281
BstDEI CTNAG 1 cut(s) 480
BstDSI CCRYGG 1 cut(s) 76
BstENI CCTNNNNNAGG 1 cut(s) 65
BstF5I GGATG 1 cut(s) 497
BstFNI CGCG 3 cut(s) 78, 379, 384
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 3 cut(s) 6, 182, 386
BstKTI GATC 2 cut(s) 141, 187
BstMAI GTCTC 3 cut(s) 58, 334, 533
BstMBI GATC 2 cut(s) 138, 184
BstMWI GCNNNNNNNGC 5 cut(s) 127, 133, 280, 434, 476
BstNI CCWGG 4 cut(s) 110, 345, 433, 562
BstPAI GACNNNNGTC 1 cut(s) 378
BstSCI CCNGG 4 cut(s) 108, 343, 431, 560
BstUI CGCG 3 cut(s) 78, 379, 384
BstV1I GCAGC 2 cut(s) 145, 440
BstV2I GAAGAC 1 cut(s) 317
BsuRI GGCC 2 cut(s) 113, 452
BtgI CCRYGG 1 cut(s) 76
BtsCI GGATG 1 cut(s) 497
BtsIMutI CAGTG 1 cut(s) 507
Cac8I GCNNGC 3 cut(s) 8, 42, 281
CfoI GCGC 3 cut(s) 6, 182, 386
Cfr13I GGNCC 3 cut(s) 112, 373, 451
Cfr42I CCGCGG 1 cut(s) 79
CseI GACGC 1 cut(s) 368
Csp6I GTAC 2 cut(s) 322, 513
CviQI GTAC 2 cut(s) 322, 513
DdeI CTNAG 1 cut(s) 480
DinI GGCGCC 1 cut(s) 181
DpnI GATC 2 cut(s) 140, 186
DpnII GATC 2 cut(s) 138, 184
DriI GACNNNNNGTC 1 cut(s) 546
Eam1105I GACNNNNNGTC 1 cut(s) 546
EciI GGCGGA 2 cut(s) 46, 377
Eco130I CCWWGG 2 cut(s) 27, 253
Eco31I GGTCTC 2 cut(s) 334, 533
Eco47I GGWCC 1 cut(s) 373
Eco72I CACGTG 1 cut(s) 219
Eco88I CYCGRG 2 cut(s) 401, 455
EcoNI CCTNNNNNAGG 1 cut(s) 65
EcoO109I RGGNCCY 1 cut(s) 373
EcoRII CCWGG 4 cut(s) 108, 343, 431, 560
EcoT14I CCWWGG 2 cut(s) 27, 253
EgeI GGCGCC 1 cut(s) 181
EheI GGCGCC 1 cut(s) 181
ErhI CCWWGG 2 cut(s) 27, 253
FaiI YATR 3 cut(s) 147, 229, 441
FalI AAGNNNNNCTT 2 cut(s) 473, 505
FaqI GGGAC 2 cut(s) 386, 418
FauI CCCGC 2 cut(s) 279, 384
Fnu4HI GCNGC 7 cut(s) 128, 131, 134, 335, 360, 429, 450
FokI GGATG 1 cut(s) 484
Fsp4HI GCNGC 7 cut(s) 128, 131, 134, 335, 360, 429, 450
FspBI CTAG 1 cut(s) 315
GlaI GCGC 3 cut(s) 5, 181, 385
GluI GCNGC 7 cut(s) 128, 131, 134, 335, 360, 429, 450
HaeII RGCGCY 1 cut(s) 183
HaeIII GGCC 2 cut(s) 113, 452
HapII CCGG 1 cut(s) 15
HgaI GACGC 1 cut(s) 368
HhaI GCGC 3 cut(s) 6, 182, 386
Hin1I GRCGYC 1 cut(s) 180
Hin6I GCGC 3 cut(s) 4, 180, 384
HinP1I GCGC 3 cut(s) 4, 180, 384
HinfI GANTC 3 cut(s) 209, 367, 557
HpaII CCGG 1 cut(s) 15
Hpy166II GTNNAC 1 cut(s) 23
Hpy188I TCNGA 4 cut(s) 208, 214, 414, 505
Hpy188III TCNNGA 3 cut(s) 193, 371, 403
Hpy8I GTNNAC 1 cut(s) 23
Hpy99I CGWCG 1 cut(s) 384
HpyCH4III ACNGT 2 cut(s) 201, 548
HpyCH4IV ACGT 2 cut(s) 218, 407
HpyCH4V TGCA 1 cut(s) 101
HpyF10VI GCNNNNNNNGC 5 cut(s) 127, 133, 280, 434, 476
HpyF3I CTNAG 1 cut(s) 480
HpySE526I ACGT 2 cut(s) 218, 407
Hsp92I GRCGYC 1 cut(s) 180
HspAI GCGC 3 cut(s) 4, 180, 384
KasI GGCGCC 1 cut(s) 179
KflI GGGWCCC 1 cut(s) 373
KspI CCGCGG 1 cut(s) 79
Kzo9I GATC 2 cut(s) 138, 184
LmnI GCTCC 3 cut(s) 31, 37, 467
Lsp1109I GCAGC 2 cut(s) 145, 440
MaeI CTAG 1 cut(s) 315
MaeII ACGT 2 cut(s) 218, 407
MalI GATC 2 cut(s) 140, 186
MboI GATC 2 cut(s) 138, 184
MboII GAAGA 3 cut(s) 313, 316, 322
MhlI GDGCHC 1 cut(s) 42
Mly113I GGCGCC 1 cut(s) 180
MlyI GAGTC 1 cut(s) 566
MmeI TCCRAC 1 cut(s) 194
MspA1I CMGCKG 2 cut(s) 78, 136
MspI CCGG 1 cut(s) 15
MspR9I CCNGG 4 cut(s) 110, 345, 433, 562
Mva1269I GAATGC 1 cut(s) 10
MvaI CCWGG 4 cut(s) 110, 345, 433, 562
MvnI CGCG 3 cut(s) 78, 379, 384
MwoI GCNNNNNNNGC 5 cut(s) 127, 133, 280, 434, 476
NarI GGCGCC 1 cut(s) 180
NdeII GATC 2 cut(s) 138, 184
NlaIV GGNNCC 3 cut(s) 181, 374, 375
PaeR7I CTCGAG 1 cut(s) 455
PcsI WCGNNNNNNNCGW 1 cut(s) 376
PctI GAATGC 1 cut(s) 10
PfeI GAWTC 2 cut(s) 209, 367
PfoI TCCNGGA 1 cut(s) 560
PkrI GCNGC 7 cut(s) 129, 132, 135, 336, 361, 430, 451
PleI GAGTC 1 cut(s) 565
PluTI GGCGCC 1 cut(s) 183
PmaCI CACGTG 1 cut(s) 219
PmlI CACGTG 1 cut(s) 219
PpsI GAGTC 1 cut(s) 565
Ppu21I YACGTR 1 cut(s) 219
PpuMI RGGWCCY 1 cut(s) 373
PshAI GACNNNNGTC 1 cut(s) 378
Psp5II RGGWCCY 1 cut(s) 373
Psp6I CCWGG 4 cut(s) 108, 343, 431, 560
PspCI CACGTG 1 cut(s) 219
PspGI CCWGG 4 cut(s) 108, 343, 431, 560
PspN4I GGNNCC 3 cut(s) 181, 374, 375
PspPI GGNCC 3 cut(s) 112, 373, 451
PspPPI RGGWCCY 1 cut(s) 373
PspXI VCTCGAGB 1 cut(s) 455
PvuII CAGCTG 1 cut(s) 136
RsaI GTAC 2 cut(s) 323, 514
RsaNI GTAC 2 cut(s) 322, 513
SacII CCGCGG 1 cut(s) 79
SatI GCNGC 7 cut(s) 128, 131, 134, 335, 360, 429, 450
Sau3AI GATC 2 cut(s) 138, 184
Sau96I GGNCC 3 cut(s) 112, 373, 451
SchI GAGTC 1 cut(s) 566
ScrFI CCNGG 4 cut(s) 110, 345, 433, 562
SduI GDGCHC 1 cut(s) 42
SetI ASST 8 cut(s) 72, 138, 221, 228, 410, 472, 481, 486
SfoI GGCGCC 1 cut(s) 181
Sfr274I CTCGAG 1 cut(s) 455
Sfr303I CCGCGG 1 cut(s) 79
SgrBI CCGCGG 1 cut(s) 79
SinI GGWCC 1 cut(s) 373
SlaI CTCGAG 1 cut(s) 455
SmlI CTYRAG 1 cut(s) 455
SmoI CTYRAG 1 cut(s) 455
SspDI GGCGCC 1 cut(s) 179
SspMI CTAG 1 cut(s) 315
StyD4I CCNGG 4 cut(s) 108, 343, 431, 560
StyI CCWWGG 2 cut(s) 27, 253
TaaI ACNGT 2 cut(s) 201, 548
TaiI ACGT 2 cut(s) 221, 410
TaqI TCGA 1 cut(s) 456
TatI WGTACW 1 cut(s) 321
TauI GCSGC 5 cut(s) 130, 133, 337, 362, 452
TfiI GAWTC 2 cut(s) 209, 367
TscAI CASTG 1 cut(s) 514
TseI GCWGC 2 cut(s) 133, 428
TspDTI ATGAA 1 cut(s) 323
TspGWI ACGGA 1 cut(s) 431
TspRI CASTG 1 cut(s) 514
VpaK11BI GGWCC 1 cut(s) 373
XagI CCTNNNNNAGG 1 cut(s) 65
XcmI CCANNNNNNNNNTGG 1 cut(s) 351
XhoI CTCGAG 1 cut(s) 455
XspI CTAG 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.