pycom09g16760

YIF1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0009482
Physical Location & Seq
Reverse (-)
17155389 .. 17158098
2710 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 810 bp
ATGTACAGTAATGTGGGAGGCCAGCCTGGGGCGGCAATGCCGACGGCATCCCCGCCGATGAATCCGTTTGGGAATTCGTTGTACGGTGCTAGTTCCGGATTAATCCGTGGTGGATTGGGTGCATATGGAGAGAAGATTTTAGGATCAAGCTCTGAGTATGTACAAAGCAATATCGGTCAAAACGGGATCGATCCGCAATACTACTTCCAAGTGAATGACCAGTATGTGAGGAACAAATTGAAGGTTGTTCTATTTCCTTTTCTCCACAGGGGTCATTGGACCAGAATAACGGAGCCAGTAGGAGGCAGGCTTTCTTATAAACCCCCAATCTATGACATAAATGCACCAGACCTATACATTCCATTTATGGCATTTGGTACCTATGTGGTTCTGGCTGGCTTCTCATTGGGTCTTCAAGGAAAGTTTAGCCCTGAAGCTCTAAACTGGTTGTTTATCAAGGGCTTGCTTGGCTGGTTTATACAAGTTGCACTACTGAAAGTATCATTGCTTTCATTGGGCGGTGGTGAGGCACCGTTGCTGGACATGGTGGCATATGCTGGGTATGCTTTCACGGGGTTGTGCTTTGCTGTACTTGGACGGATTATGTTGAGCTACTCATACTACTTTTTGATGCCTTGGACGTGTTTATGCATGGGAGTCTTCTTGGTGAAGACAATGAAGAGAGTTCTATTCGCCGAGGTGAGGAGTTATGATTCAAGCAAGCAGCATTACCTGTTGCTGTTTATTGCTTTGGCTCAGTTACCTCTGTTTACGTGGCTTGGCAACGTTACTGTTAATTGGCTCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YIF1

Protein Analysis

270

Amino Acids

29.77

Weight (kDa)

9.12

Isoelectric Point (pI)

41.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YIF1 PF03878 41 - 260 5.5e-54 YIF1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 318
Acc65I GGTACC 1 cut(s) 377
AccB1I GGYRCC 2 cut(s) 377, 529
AccIII TCCGGA 1 cut(s) 95
AciI CCGC 4 cut(s) 32, 53, 194, 519
AclI AACGTT 1 cut(s) 786
AclWI GGATC 3 cut(s) 151, 185, 194
AcsI RAATTY 1 cut(s) 73
AcuI CTGAAG 1 cut(s) 453
AfaI GTAC 5 cut(s) 5, 83, 162, 379, 591
AfiI CCNNNNNNNGG 3 cut(s) 28, 302, 702
AflIII ACRYGT 1 cut(s) 641
AgsI TTSAA 3 cut(s) 241, 416, 717
AjiI CACGTC 1 cut(s) 642
AjnI CCWGG 1 cut(s) 25
AluBI AGCT 3 cut(s) 150, 437, 612
AluI AGCT 3 cut(s) 150, 437, 612
AlwI GGATC 3 cut(s) 151, 185, 194
Aor13HI TCCGGA 1 cut(s) 95
AoxI GGCC 1 cut(s) 19
ApeKI GCWGC 1 cut(s) 724
ApoI RAATTY 1 cut(s) 73
AseI ATTAAT 1 cut(s) 101
Asp718I GGTACC 1 cut(s) 377
AspS9I GGNCC 1 cut(s) 279
AsuHPI GGTGA 3 cut(s) 536, 679, 712
AvaII GGWCC 1 cut(s) 279
BaeI ACNNNNGTAYC 2 cut(s) 369, 402
BanI GGYRCC 2 cut(s) 377, 529
BbsI GAAGAC 3 cut(s) 404, 652, 677
BbvI GCAGC 1 cut(s) 736
BceAI ACGGC 1 cut(s) 60
BciT130I CCWGG 1 cut(s) 27
BfaI CTAG 1 cut(s) 90
BisI GCNGC 2 cut(s) 33, 725
BlsI GCNGC 2 cut(s) 34, 726
Bme1390I CCNGG 1 cut(s) 27
Bme18I GGWCC 1 cut(s) 279
BmgBI CACGTC 1 cut(s) 642
BmgT120I GGNCC 1 cut(s) 279
BmiI GGNNCC 3 cut(s) 294, 379, 531
BmrFI CCNGG 1 cut(s) 27
BmsI GCATC 2 cut(s) 56, 621
BpiI GAAGAC 3 cut(s) 404, 652, 677
Bsa29I ATCGAT 1 cut(s) 189
BsaAI YACGTR 1 cut(s) 774
BsaJI CCNNGG 4 cut(s) 26, 106, 635, 696
BsaWI WCCGGW 1 cut(s) 95
Bsc4I CCNNNNNNNGG 3 cut(s) 28, 302, 702
Bse1I ACTGG 3 cut(s) 220, 296, 449
Bse3DI GCAATG 2 cut(s) 42, 503
BseAI TCCGGA 1 cut(s) 95
BseBI CCWGG 1 cut(s) 27
BseCI ATCGAT 1 cut(s) 189
BseDI CCNNGG 4 cut(s) 26, 106, 635, 696
BseGI GGATG 1 cut(s) 47
BseLI CCNNNNNNNGG 3 cut(s) 28, 302, 702
BseMI GCAATG 2 cut(s) 42, 503
BseMII CTCAG 2 cut(s) 144, 770
BseNI ACTGG 3 cut(s) 220, 296, 449
BseRI GAGGAG 1 cut(s) 718
BseXI GCAGC 1 cut(s) 736
BseYI CCCAGC 1 cut(s) 557
BshFI GGCC 1 cut(s) 21
BshNI GGYRCC 2 cut(s) 377, 529
BshVI ATCGAT 1 cut(s) 189
BsiSI CCGG 1 cut(s) 96
BslI CCNNNNNNNGG 3 cut(s) 28, 302, 702
BsnI GGCC 1 cut(s) 21
Bsp13I TCCGGA 1 cut(s) 95
Bsp1407I TGTACA 2 cut(s) 3, 160
Bsp143I GATC 3 cut(s) 143, 186, 190
BspACI CCGC 4 cut(s) 32, 53, 194, 519
BspANI GGCC 1 cut(s) 21
BspCNI CTCAG 2 cut(s) 145, 769
BspDI ATCGAT 1 cut(s) 189
BspEI TCCGGA 1 cut(s) 95
BspLI GGNNCC 3 cut(s) 294, 379, 531
BspPI GGATC 3 cut(s) 151, 185, 194
BspT107I GGYRCC 2 cut(s) 377, 529
BsrDI GCAATG 2 cut(s) 42, 503
BsrGI TGTACA 2 cut(s) 3, 160
BsrI ACTGG 3 cut(s) 220, 296, 449
BssECI CCNNGG 4 cut(s) 26, 106, 635, 696
BssMI GATC 3 cut(s) 143, 186, 190
BssT1I CCWWGG 1 cut(s) 635
Bst2UI CCWGG 1 cut(s) 27
Bst4CI ACNGT 4 cut(s) 8, 86, 534, 793
Bst6I CTCTTC 1 cut(s) 674
BstAUI TGTACA 2 cut(s) 3, 160
BstBAI YACGTR 1 cut(s) 774
BstC8I GCNNGC 5 cut(s) 23, 308, 397, 464, 722
BstDEI CTNAG 2 cut(s) 153, 756
BstDSI CCRYGG 1 cut(s) 106
BstF5I GGATG 1 cut(s) 47
BstKTI GATC 3 cut(s) 146, 189, 193
BstMBI GATC 3 cut(s) 143, 186, 190
BstMWI GCNNNNNNNGC 2 cut(s) 468, 563
BstNI CCWGG 1 cut(s) 27
BstSCI CCNGG 1 cut(s) 25
BstV1I GCAGC 1 cut(s) 736
BstV2I GAAGAC 3 cut(s) 404, 652, 677
Bsu15I ATCGAT 1 cut(s) 189
BsuRI GGCC 1 cut(s) 21
BsuTUI ATCGAT 1 cut(s) 189
BtgI CCRYGG 1 cut(s) 106
BtrI CACGTC 1 cut(s) 642
BtsCI GGATG 1 cut(s) 47
Cac8I GCNNGC 5 cut(s) 23, 308, 397, 464, 722
Cfr13I GGNCC 1 cut(s) 279
ClaI ATCGAT 1 cut(s) 189
Csp6I GTAC 5 cut(s) 4, 82, 161, 378, 590
CviAII CATG 2 cut(s) 544, 652
CviQI GTAC 5 cut(s) 4, 82, 161, 378, 590
DdeI CTNAG 2 cut(s) 153, 756
DpnI GATC 3 cut(s) 145, 188, 192
DpnII GATC 3 cut(s) 143, 186, 190
Eam1104I CTCTTC 1 cut(s) 674
EarI CTCTTC 1 cut(s) 674
Eco130I CCWWGG 1 cut(s) 635
Eco47I GGWCC 1 cut(s) 279
Eco57I CTGAAG 1 cut(s) 453
EcoRI GAATTC 1 cut(s) 73
EcoRII CCWGG 1 cut(s) 25
EcoT14I CCWWGG 1 cut(s) 635
EcoT22I ATGCAT 1 cut(s) 653
ErhI CCWWGG 1 cut(s) 635
FaeI CATG 2 cut(s) 547, 655
FatI CATG 2 cut(s) 543, 651
FauI CCCGC 1 cut(s) 60
FauNDI CATATG 2 cut(s) 124, 553
Fnu4HI GCNGC 2 cut(s) 33, 725
FokI GGATG 1 cut(s) 34
Fsp4HI GCNGC 2 cut(s) 33, 725
FspBI CTAG 1 cut(s) 90
GluI GCNGC 2 cut(s) 33, 725
GsaI CCCAGC 1 cut(s) 561
HaeIII GGCC 1 cut(s) 21
HapII CCGG 1 cut(s) 96
Hin1II CATG 2 cut(s) 547, 655
HinfI GANTC 3 cut(s) 61, 657, 713
HpaII CCGG 1 cut(s) 96
HphI GGTGA 3 cut(s) 536, 679, 712
Hpy166II GTNNAC 1 cut(s) 771
Hpy188I TCNGA 1 cut(s) 154
Hpy188III TCNNGA 1 cut(s) 96
Hpy8I GTNNAC 1 cut(s) 771
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 1 cut(s) 235
HpyCH4III ACNGT 4 cut(s) 8, 86, 534, 793
HpyCH4IV ACGT 3 cut(s) 641, 773, 786
HpyCH4V TGCA 4 cut(s) 122, 344, 488, 651
HpyF10VI GCNNNNNNNGC 2 cut(s) 468, 563
HpyF3I CTNAG 2 cut(s) 153, 756
HpySE526I ACGT 3 cut(s) 641, 773, 786
Hsp92II CATG 2 cut(s) 547, 655
Kpn2I TCCGGA 1 cut(s) 95
KpnI GGTACC 1 cut(s) 381
Kzo9I GATC 3 cut(s) 143, 186, 190
LmnI GCTCC 1 cut(s) 292
Lsp1109I GCAGC 1 cut(s) 736
LweI GCATC 2 cut(s) 56, 621
MaeI CTAG 1 cut(s) 90
MaeII ACGT 3 cut(s) 641, 773, 786
MaeIII GTNAC 2 cut(s) 759, 787
MalI GATC 3 cut(s) 145, 188, 192
MboI GATC 3 cut(s) 143, 186, 190
MboII GAAGA 5 cut(s) 145, 404, 652, 682, 691
MluCI AATT 3 cut(s) 73, 236, 796
MlyI GAGTC 1 cut(s) 666
MnlI CCTC 7 cut(s) 11, 222, 296, 520, 691, 696, 774
Mph1103I ATGCAT 1 cut(s) 653
MroI TCCGGA 1 cut(s) 95
MseI TTAA 2 cut(s) 101, 795
MspI CCGG 1 cut(s) 96
MspR9I CCNGG 1 cut(s) 27
MvaI CCWGG 1 cut(s) 27
MwoI GCNNNNNNNGC 2 cut(s) 468, 563
NdeI CATATG 2 cut(s) 124, 553
NdeII GATC 3 cut(s) 143, 186, 190
NlaIII CATG 2 cut(s) 547, 655
NlaIV GGNNCC 3 cut(s) 294, 379, 531
NmeAIII GCCGAG 1 cut(s) 721
NsiI ATGCAT 1 cut(s) 653
PfeI GAWTC 2 cut(s) 61, 713
PkrI GCNGC 2 cut(s) 34, 726
PleI GAGTC 1 cut(s) 665
PpsI GAGTC 1 cut(s) 665
Ppu21I YACGTR 1 cut(s) 774
PshBI ATTAAT 1 cut(s) 101
PsiI TTATAA 1 cut(s) 318
Psp1406I AACGTT 1 cut(s) 786
Psp6I CCWGG 1 cut(s) 25
PspFI CCCAGC 1 cut(s) 557
PspGI CCWGG 1 cut(s) 25
PspN4I GGNNCC 3 cut(s) 294, 379, 531
PspPI GGNCC 1 cut(s) 279
RsaI GTAC 5 cut(s) 5, 83, 162, 379, 591
RsaNI GTAC 5 cut(s) 4, 82, 161, 378, 590
SaqAI TTAA 2 cut(s) 101, 795
SatI GCNGC 2 cut(s) 33, 725
Sau3AI GATC 3 cut(s) 143, 186, 190
Sau96I GGNCC 1 cut(s) 279
SchI GAGTC 1 cut(s) 666
ScrFI CCNGG 1 cut(s) 27
SfaNI GCATC 2 cut(s) 56, 621
SinI GGWCC 1 cut(s) 279
Sse9I AATT 3 cut(s) 73, 236, 796
SsiI CCGC 4 cut(s) 32, 53, 194, 519
SspMI CTAG 1 cut(s) 90
StyD4I CCNGG 1 cut(s) 25
StyI CCWWGG 1 cut(s) 635
TaaI ACNGT 4 cut(s) 8, 86, 534, 793
TaiI ACGT 3 cut(s) 644, 776, 789
TaqI TCGA 1 cut(s) 189
TaqII GACCGA 1 cut(s) 164
TasI AATT 3 cut(s) 73, 236, 796
TatI WGTACW 3 cut(s) 3, 160, 589
TauI GCSGC 1 cut(s) 35
TfiI GAWTC 2 cut(s) 61, 713
Tru1I TTAA 2 cut(s) 101, 795
Tru9I TTAA 2 cut(s) 101, 795
TseI GCWGC 1 cut(s) 724
TspDTI ATGAA 3 cut(s) 74, 501, 692
TspGWI ACGGA 4 cut(s) 54, 95, 305, 613
VpaK11BI GGWCC 1 cut(s) 279
VspI ATTAAT 1 cut(s) 101
XapI RAATTY 1 cut(s) 73
XspI CTAG 1 cut(s) 90
Zsp2I ATGCAT 1 cut(s) 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.