pycom09g17620

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0012800
Physical Location & Seq
Reverse (-)
18163641 .. 18167863
4223 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g17620.1

Sequence Viewer

Length: 1242 bp
ATGGATTCCCGTTCATCATCCGACGGTCACAATTCGACTCCGGACTCACCCTCTTCCACCTTCTCCTCCTCCTCAGCCGTCCATCGCGCTCTGCAACTCATCCAATCCGACGACCAGGACTCCAAGCTCCAGGGCGCCCAGGAAATCCGGCGCCTCACCAAGACCTCGCAGCGCTGTCGGCGCCAGCTCTCCGACTCGGTGGGCCCACTCGTCTCCATGCTCCGAGTTTGCGACTCGCCCGAGTCCCATGAATCGGCCCTACTCGCCCTCCTCAACCTCGCCGTCAAGGATGAGACATGCTGTAATATTTTTGCCACAATCTTTGTGTTTCTGCTGCTGATTTTTCTTCTCAATATTTGGTCTCTCAGGAACAAAATTAAGATAGTGGAAGCTGGTGCTTTAGAACCAATAATTACTTTCCTTCCATCACAAAGTTGCAACCTTCAGGAGTATGCAACTGCAGCTCTACTCACCCTATCGGCTTCTGCCATCAACAAGCCAGTTATAGGTGCTTCTGGTGCCATTCCTCTTCTCGTCGAAATCCTTAGACATGGAGCCCCACAAGCTAAAGTCGATTCAGTAATGGCGCTTTCCAATCTCTCCACACACCCTGCAAACCTTAGCATCATTCTTGAAGCAAAACCAATTCCGTCCATAGTTTCCTTATTGAAACCCTGCAAGAAATCTTCAAAAACGGCTGAGAAATGCTGTGCTCTGATAGAATCTCTGGTGGGATTCGATGAGGGCAGAACTGCCCTCACATCTGAAGAAGGCGGAGTGCTAGCAGTGATTGAGGTGCTTGAAAATGGATCGATCCAAAGTCGGGAGCACGCTGTAAGTGCTCTACTAACAATGTGTCAAAGTGACAGGTGCAGATATAGAGAAGCGATCCTTAAGGACGGGGTAATCCCTGGACTTCTTGAGCTGACAGTTCAAGGCACGCCCAAGTCTCAGACAAAAGCGCATGCGCTTTTGCGCTTATTGAGGGACTCTCCGTACCCCAGGTCAGAGCTTCAGGCAGACACACTGGAGAACATTGTATGCAACATCATCTCTCAGATTGACAGTGATGAACAGTCCAGTAAAGCGAAGAAAATGCTGGCCGACATGGTTCAAGTCAGCATGGAACAAAGCTTGAGACATTTACAGCAAAGGGCTCTGGACTTACCCGATCGTCCTGAAACCAACTTGTTTATCACTGAAAAGGTTGCGAGCGCCGGGGCGGTGAACGAGTTGCCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm

Protein Analysis

414

Amino Acids

44.76

Weight (kDa)

5.96

Isoelectric Point (pI)

61.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_PUB PF25598 40 - 332 8.6e-15 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 196 - 373 4.5e-08 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012800)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03440
fragaria_vesca FvH4_1g29130
malus_domestica MD09G1261900.v1.1 MD17G1256900.v1.1
prunus_persica Prupe.3G159600_v2.0.a1
pyrus_communis pycom09g17620 pycom17g25920
rosa_chinensis RchiOBHm_Chr3g0493501 RchiOBHm_Chr3g0493511
rosa_laevigata RLG00000022710
rosa_multiflora Rmu_co8033802.1_g000001
rosa_roxburghii Rroxscaffold_6G00391540
rosa_rugosa Rorug03G0261600
rosa_samantha Rh3AG310500 Rh3BG346700 Rh3CG344500 Rh3DG345800
rosa_wichuraiana Rw3G027290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 134, 150, 180, 518
AccII CGCG 1 cut(s) 87
AccIII TCCGGA 1 cut(s) 40
AciI CCGC 2 cut(s) 774, 1223
AclWI GGATC 3 cut(s) 808, 817, 883
AcoI YGGCCR 1 cut(s) 1101
AcuI CTGAAG 3 cut(s) 428, 786, 998
AcyI GRCGYC 3 cut(s) 135, 151, 181
AfaI GTAC 1 cut(s) 998
AfeI AGCGCT 1 cut(s) 173
AfiI CCNNNNNNNGG 3 cut(s) 253, 506, 823
AflII CTTAAG 1 cut(s) 893
AgsI TTSAA 6 cut(s) 635, 670, 690, 803, 935, 1115
AjnI CCWGG 5 cut(s) 114, 129, 138, 910, 1001
AluBI AGCT 8 cut(s) 127, 187, 392, 464, 566, 925, 1012, 1134
AluI AGCT 8 cut(s) 127, 187, 392, 464, 566, 925, 1012, 1134
Alw21I GWGCWC 3 cut(s) 715, 831, 844
Alw26I GTCTC 5 cut(s) 217, 287, 366, 954, 1132
AlwI GGATC 3 cut(s) 808, 817, 883
Ama87I CYCGRG 1 cut(s) 239
Aor13HI TCCGGA 1 cut(s) 40
Aor51HI AGCGCT 1 cut(s) 173
AoxI GGCC 3 cut(s) 202, 255, 1101
ApaI GGGCCC 1 cut(s) 206
ApeKI GCWGC 3 cut(s) 169, 334, 461
AspS9I GGNCC 3 cut(s) 202, 203, 256
AsuC2I CCSGG 1 cut(s) 1219
AsuHPI GGTGA 4 cut(s) 39, 148, 463, 1237
AsuNHI GCTAGC 1 cut(s) 781
AvaI CYCGRG 1 cut(s) 239
BaeGI GKGCMC 1 cut(s) 206
BanI GGYRCC 4 cut(s) 134, 150, 180, 518
BanII GRGCYC 3 cut(s) 206, 559, 1159
Bbv12I GWGCWC 3 cut(s) 715, 831, 844
BbvCI CCTCAGC 1 cut(s) 73
BbvI GCAGC 3 cut(s) 181, 321, 473
BccI CCATC 3 cut(s) 90, 433, 497
BceAI ACGGC 4 cut(s) 62, 266, 711, 1222
BcgI CGANNNNNNTGC 4 cut(s) 158, 192, 1078, 1112
BciT130I CCWGG 5 cut(s) 116, 131, 140, 912, 1003
BcnI CCSGG 1 cut(s) 1219
BcoDI GTCTC 5 cut(s) 217, 287, 366, 954, 1132
BfaI CTAG 1 cut(s) 782
BfmI CTRYAG 1 cut(s) 459
BfoI RGCGCY 6 cut(s) 138, 154, 175, 184, 590, 1218
BfrI CTTAAG 1 cut(s) 893
BisI GCNGC 3 cut(s) 170, 335, 462
BlsI GCNGC 3 cut(s) 171, 336, 463
Bme1390I CCNGG 6 cut(s) 116, 131, 140, 912, 1003, 1219
BmeT110I CYCGRG 1 cut(s) 239
BmgT120I GGNCC 3 cut(s) 202, 203, 256
BmiI GGNNCC 6 cut(s) 136, 152, 182, 204, 520, 556
BmrFI CCNGG 6 cut(s) 116, 131, 140, 912, 1003, 1219
BmsI GCATC 1 cut(s) 633
BmtI GCTAGC 1 cut(s) 785
BplI GAGNNNNNCTC 2 cut(s) 976, 1008
BpmI CTGGAG 2 cut(s) 113, 1049
Bpu10I CCTNAGC 2 cut(s) 73, 620
BpuEI CTTGAG 2 cut(s) 941, 1156
BpuMI CCSGG 1 cut(s) 1219
Bsa29I ATCGAT 1 cut(s) 812
BsaHI GRCGYC 3 cut(s) 135, 151, 181
BsaI GGTCTC 1 cut(s) 366
BsaJI CCNNGG 5 cut(s) 130, 138, 910, 1001, 1218
BsaWI WCCGGW 1 cut(s) 40
BsaXI ACNNNNNCTCC 4 cut(s) 104, 134, 252, 282
Bsc4I CCNNNNNNNGG 3 cut(s) 253, 506, 823
Bse1I ACTGG 3 cut(s) 500, 1032, 1080
BseAI TCCGGA 1 cut(s) 40
BseBI CCWGG 5 cut(s) 116, 131, 140, 912, 1003
BseCI ATCGAT 1 cut(s) 812
BseDI CCNNGG 5 cut(s) 130, 138, 910, 1001, 1218
BseGI GGATG 3 cut(s) 17, 99, 295
BseLI CCNNNNNNNGG 3 cut(s) 253, 506, 823
BseMII CTCAG 5 cut(s) 87, 379, 690, 965, 1070
BseNI ACTGG 3 cut(s) 500, 1032, 1080
BseRI GAGGAG 4 cut(s) 55, 58, 61, 260
BseSI GKGCMC 1 cut(s) 206
BseXI GCAGC 3 cut(s) 181, 321, 473
BsgI GTGCAG 1 cut(s) 892
Bsh1236I CGCG 1 cut(s) 87
Bsh1285I CGRYCG 1 cut(s) 1174
BshFI GGCC 3 cut(s) 204, 257, 1103
BshNI GGYRCC 4 cut(s) 134, 150, 180, 518
BshVI ATCGAT 1 cut(s) 812
BsiEI CGRYCG 1 cut(s) 1174
BsiHKAI GWGCWC 3 cut(s) 715, 831, 844
BsiHKCI CYCGRG 1 cut(s) 239
BsiSI CCGG 3 cut(s) 41, 148, 1218
BslFI GGGAC 2 cut(s) 229, 1001
BslI CCNNNNNNNGG 3 cut(s) 253, 506, 823
BsmAI GTCTC 5 cut(s) 217, 287, 366, 954, 1132
BsmBI CGTCTC 1 cut(s) 217
BsmFI GGGAC 2 cut(s) 229, 1001
BsnI GGCC 3 cut(s) 204, 257, 1103
Bso31I GGTCTC 1 cut(s) 366
BsoBI CYCGRG 1 cut(s) 239
Bsp120I GGGCCC 1 cut(s) 202
Bsp1286I GDGCHC 6 cut(s) 206, 559, 715, 831, 844, 1159
Bsp13I TCCGGA 1 cut(s) 40
Bsp143I GATC 4 cut(s) 809, 813, 888, 1171
BspACI CCGC 2 cut(s) 774, 1223
BspANI GGCC 3 cut(s) 204, 257, 1103
BspCNI CTCAG 5 cut(s) 86, 378, 691, 964, 1069
BspDI ATCGAT 1 cut(s) 812
BspEI TCCGGA 1 cut(s) 40
BspFNI CGCG 1 cut(s) 87
BspLI GGNNCC 6 cut(s) 136, 152, 182, 204, 520, 556
BspMAI CTGCAG 1 cut(s) 463
BspOI GCTAGC 1 cut(s) 785
BspPI GGATC 3 cut(s) 808, 817, 883
BspT107I GGYRCC 4 cut(s) 134, 150, 180, 518
BspTI CTTAAG 1 cut(s) 893
BspTNI GGTCTC 1 cut(s) 366
BsrI ACTGG 3 cut(s) 500, 1032, 1080
BssECI CCNNGG 5 cut(s) 130, 138, 910, 1001, 1218
BssMI GATC 4 cut(s) 809, 813, 888, 1171
BssNI GRCGYC 3 cut(s) 135, 151, 181
Bst2UI CCWGG 5 cut(s) 116, 131, 140, 912, 1003
Bst4CI ACNGT 4 cut(s) 26, 931, 1067, 1077
Bst6I CTCTTC 2 cut(s) 58, 534
BstACI GRCGYC 3 cut(s) 135, 151, 181
BstAFI CTTAAG 1 cut(s) 893
BstC8I GCNNGC 7 cut(s) 185, 783, 831, 941, 966, 1101, 1213
BstDEI CTNAG 7 cut(s) 73, 365, 545, 620, 699, 951, 1056
BstF5I GGATG 3 cut(s) 17, 99, 295
BstFNI CGCG 1 cut(s) 87
BstH2I RGCGCY 6 cut(s) 138, 154, 175, 184, 590, 1218
BstKTI GATC 4 cut(s) 812, 816, 891, 1174
BstMAI GTCTC 5 cut(s) 217, 287, 366, 954, 1132
BstMBI GATC 4 cut(s) 809, 813, 888, 1171
BstMCI CGRYCG 1 cut(s) 1174
BstMWI GCNNNNNNNGC 7 cut(s) 178, 180, 263, 461, 518, 563, 839
BstNI CCWGG 5 cut(s) 116, 131, 140, 912, 1003
BstNSI RCATGY 2 cut(s) 300, 968
BstSCI CCNGG 6 cut(s) 114, 129, 138, 910, 1001, 1217
BstSFI CTRYAG 1 cut(s) 459
BstSLI GKGCMC 1 cut(s) 206
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 3 cut(s) 181, 321, 473
Bsu15I ATCGAT 1 cut(s) 812
BsuRI GGCC 3 cut(s) 204, 257, 1103
BsuTUI ATCGAT 1 cut(s) 812
BtgZI GCGATG 1 cut(s) 68
BtsCI GGATG 3 cut(s) 17, 99, 295
BtsI GCAGTG 1 cut(s) 792
BtsIMutI CAGTG 4 cut(s) 792, 1025, 1072, 1197
Cac8I GCNNGC 7 cut(s) 185, 783, 831, 941, 966, 1101, 1213
Cfr13I GGNCC 3 cut(s) 202, 203, 256
ClaI ATCGAT 1 cut(s) 812
Csp6I GTAC 1 cut(s) 997
CspCI CAANNNNNGTGG 2 cut(s) 304, 339
CviAII CATG 7 cut(s) 217, 248, 297, 551, 965, 1108, 1123
CviQI GTAC 1 cut(s) 997
DdeI CTNAG 7 cut(s) 73, 365, 545, 620, 699, 951, 1056
DinI GGCGCC 3 cut(s) 136, 152, 182
DpnI GATC 4 cut(s) 811, 815, 890, 1173
DpnII GATC 4 cut(s) 809, 813, 888, 1171
EaeI YGGCCR 1 cut(s) 1101
Eam1104I CTCTTC 2 cut(s) 58, 534
EarI CTCTTC 2 cut(s) 58, 534
EciI GGCGGA 1 cut(s) 789
Eco24I GRGCYC 3 cut(s) 206, 559, 1159
Eco31I GGTCTC 1 cut(s) 366
Eco47III AGCGCT 1 cut(s) 173
Eco57I CTGAAG 3 cut(s) 428, 786, 998
Eco88I CYCGRG 1 cut(s) 239
EcoRII CCWGG 5 cut(s) 114, 129, 138, 910, 1001
EcoT38I GRGCYC 3 cut(s) 206, 559, 1159
EgeI GGCGCC 3 cut(s) 136, 152, 182
EheI GGCGCC 3 cut(s) 136, 152, 182
Esp3I CGTCTC 1 cut(s) 217
FaeI CATG 7 cut(s) 220, 251, 300, 554, 968, 1111, 1126
FalI AAGNNNNNCTT 2 cut(s) 876, 908
FaqI GGGAC 2 cut(s) 229, 1001
FatI CATG 7 cut(s) 216, 247, 296, 550, 964, 1107, 1122
Fnu4HI GCNGC 3 cut(s) 170, 335, 462
FokI GGATG 3 cut(s) 4, 86, 302
FriOI GRGCYC 3 cut(s) 206, 559, 1159
Fsp4HI GCNGC 3 cut(s) 170, 335, 462
FspBI CTAG 1 cut(s) 782
GluI GCNGC 3 cut(s) 170, 335, 462
GsuI CTGGAG 2 cut(s) 113, 1049
HaeII RGCGCY 6 cut(s) 138, 154, 175, 184, 590, 1218
HaeIII GGCC 3 cut(s) 204, 257, 1103
HapII CCGG 3 cut(s) 41, 148, 1218
Hin1I GRCGYC 3 cut(s) 135, 151, 181
Hin1II CATG 7 cut(s) 220, 251, 300, 554, 968, 1111, 1126
HindIII AAGCTT 1 cut(s) 1132
HpaII CCGG 3 cut(s) 41, 148, 1218
HphI GGTGA 4 cut(s) 39, 148, 463, 1237
Hpy166II GTNNAC 1 cut(s) 1228
Hpy188I TCNGA 9 cut(s) 22, 109, 193, 224, 717, 766, 954, 1009, 1059
Hpy188III TCNNGA 8 cut(s) 41, 367, 446, 632, 824, 920, 1160, 1178
Hpy8I GTNNAC 1 cut(s) 1228
Hpy99I CGWCG 3 cut(s) 26, 113, 539
HpyAV CCTTC 4 cut(s) 70, 431, 452, 764
HpyCH4III ACNGT 4 cut(s) 26, 931, 1067, 1077
HpyCH4V TGCA 8 cut(s) 94, 438, 455, 461, 614, 678, 873, 1044
HpyF10VI GCNNNNNNNGC 7 cut(s) 178, 180, 263, 461, 518, 563, 839
HpyF3I CTNAG 7 cut(s) 73, 365, 545, 620, 699, 951, 1056
Hsp92I GRCGYC 3 cut(s) 135, 151, 181
Hsp92II CATG 7 cut(s) 220, 251, 300, 554, 968, 1111, 1126
KasI GGCGCC 3 cut(s) 134, 150, 180
Kpn2I TCCGGA 1 cut(s) 40
Kzo9I GATC 4 cut(s) 809, 813, 888, 1171
LmnI GCTCC 4 cut(s) 132, 225, 554, 826
Lsp1109I GCAGC 3 cut(s) 181, 321, 473
LweI GCATC 1 cut(s) 633
MaeI CTAG 1 cut(s) 782
MaeIII GTNAC 2 cut(s) 26, 863
MalI GATC 4 cut(s) 811, 815, 890, 1173
MboI GATC 4 cut(s) 809, 813, 888, 1171
MboII GAAGA 6 cut(s) 45, 338, 521, 678, 779, 1102
MhlI GDGCHC 6 cut(s) 206, 559, 715, 831, 844, 1159
MluCI AATT 4 cut(s) 31, 375, 411, 645
Mly113I GGCGCC 3 cut(s) 135, 151, 181
MlyI GAGTC 7 cut(s) 31, 38, 113, 188, 227, 251, 983
MmeI TCCRAC 3 cut(s) 45, 132, 216
MroI TCCGGA 1 cut(s) 40
MseI TTAA 2 cut(s) 378, 894
MspCI CTTAAG 1 cut(s) 893
MspI CCGG 3 cut(s) 41, 148, 1218
MspR9I CCNGG 6 cut(s) 116, 131, 140, 912, 1003, 1219
MvaI CCWGG 5 cut(s) 116, 131, 140, 912, 1003
MvnI CGCG 1 cut(s) 87
MwoI GCNNNNNNNGC 7 cut(s) 178, 180, 263, 461, 518, 563, 839
NarI GGCGCC 3 cut(s) 135, 151, 181
NciI CCSGG 1 cut(s) 1219
NdeII GATC 4 cut(s) 809, 813, 888, 1171
NheI GCTAGC 1 cut(s) 781
NlaIII CATG 7 cut(s) 220, 251, 300, 554, 968, 1111, 1126
NlaIV GGNNCC 6 cut(s) 136, 152, 182, 204, 520, 556
NmuCI GTSAC 2 cut(s) 26, 863
NspI RCATGY 2 cut(s) 300, 968
PaeI GCATGC 1 cut(s) 968
PfeI GAWTC 5 cut(s) 5, 251, 575, 722, 735
PkrI GCNGC 3 cut(s) 171, 336, 463
Ple19I CGATCG 1 cut(s) 1174
PleI GAGTC 7 cut(s) 31, 38, 113, 188, 227, 250, 983
PluTI GGCGCC 3 cut(s) 138, 154, 184
PpsI GAGTC 7 cut(s) 31, 38, 113, 188, 227, 250, 983
Psp6I CCWGG 5 cut(s) 114, 129, 138, 910, 1001
PspGI CCWGG 5 cut(s) 114, 129, 138, 910, 1001
PspN4I GGNNCC 6 cut(s) 136, 152, 182, 204, 520, 556
PspOMI GGGCCC 1 cut(s) 202
PspPI GGNCC 3 cut(s) 202, 203, 256
PstI CTGCAG 1 cut(s) 463
PvuI CGATCG 1 cut(s) 1174
RsaI GTAC 1 cut(s) 998
RsaNI GTAC 1 cut(s) 997
SaqAI TTAA 2 cut(s) 378, 894
SatI GCNGC 3 cut(s) 170, 335, 462
Sau3AI GATC 4 cut(s) 809, 813, 888, 1171
Sau96I GGNCC 3 cut(s) 202, 203, 256
SchI GAGTC 7 cut(s) 31, 38, 113, 188, 227, 251, 983
ScrFI CCNGG 6 cut(s) 116, 131, 140, 912, 1003, 1219
SduI GDGCHC 6 cut(s) 206, 559, 715, 831, 844, 1159
SfaNI GCATC 1 cut(s) 633
SfcI CTRYAG 1 cut(s) 459
SfoI GGCGCC 3 cut(s) 136, 152, 182
SmlI CTYRAG 3 cut(s) 893, 920, 1135
SmoI CTYRAG 3 cut(s) 893, 920, 1135
SphI GCATGC 1 cut(s) 968
Sse9I AATT 4 cut(s) 31, 375, 411, 645
SsiI CCGC 2 cut(s) 774, 1223
SspDI GGCGCC 3 cut(s) 134, 150, 180
SspI AATATT 2 cut(s) 307, 355
SspMI CTAG 1 cut(s) 782
StyD4I CCNGG 6 cut(s) 114, 129, 138, 910, 1001, 1217
TaaI ACNGT 4 cut(s) 26, 931, 1067, 1077
TaqI TCGA 5 cut(s) 35, 537, 573, 738, 812
TasI AATT 4 cut(s) 31, 375, 411, 645
TfiI GAWTC 5 cut(s) 5, 251, 575, 722, 735
Tru1I TTAA 2 cut(s) 378, 894
Tru9I TTAA 2 cut(s) 378, 894
TscAI CASTG 4 cut(s) 792, 1032, 1072, 1204
TseFI GTSAC 2 cut(s) 26, 863
TseI GCWGC 3 cut(s) 169, 334, 461
Tsp45I GTSAC 2 cut(s) 26, 863
TspDTI ATGAA 2 cut(s) 264, 1086
TspGWI ACGGA 2 cut(s) 639, 984
TspRI CASTG 4 cut(s) 792, 1032, 1072, 1204
Vha464I CTTAAG 1 cut(s) 893
XceI RCATGY 2 cut(s) 300, 968
XspI CTAG 1 cut(s) 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.