RLG00000022710

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0012800
Physical Location & Seq
Reverse (-)
10223167 .. 10226316
3150 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022710

Sequence Viewer

Length: 1155 bp
ATGGATTCCCACTCCTCCTCCTCATCATCATCATCATCCGACGGTGGTCGGAGTGGTACTTCTTGCGGCTCGCCCTCCCCATCCGTCCGTCGTGCGTTGGAACTAATCCAATCGGACGACCACGCTTTGAAGCTCCAGGGTGCCCAGGAAATTCGACGCCTCACCAAAACCTCTCAGAGGTGTCGGCGCCAACTCTCCGACTCTGTGGGCCCACTCGTCTCCATGCTCCGAGTTCACGACTCACCCGACTTTCATGAGTCCGCCCTCCTCGCCCTCCTCAACCTCGCCGTCAAGGATGACACGAACAAAATAAAGATTGTGGAAGCTGGTGCTTTAGAACCGATAATTACATTCCTTCAGTCAGAAAATTCCAATCTGCAGGAGTATGCAACTGCATCTCTAGTCACCTTATCAGCTTCTGCTTCCAACAAGCCAGTCATAAGTGCCTGCGGTGCCATTCCTCTTCTTGTTGAAATCCTTAGAGATGGAAGCCCACAAGCCAAAGTTGATTCTGTTATGGCTCTTTCCAATCTCTCAACCGACCCAATTAACCTTAGCATCATTCTTGAATCGAAACCAGTCCCTTGTATAGTTACTTTGTTAAAAACCTGCAAAAAATCTTCAAAGACAGCTGAAAAATGCTGTGCTCTAATAGAATCTTTAGTGGGCTTTGATGAGGGTAGAACTGCCTTGACATCCGAAGAAGGTGGAGTGCTTGCAGTTATTGAGGTGCTTGAAAGTGGATCACTTCAAAGTCGAGAGCATGCTGTTGGTGCTCTACTAACAATGTGTCAAAGTGACAGATGCAAATATAGAGAAGCACTTCTTAAAGAAGGGGTAATCCCTGGACTTCTTGAGCTCACTGTTCAAGGAACAGCCAAGTCTCAGTCAAAAGCACAGACACTTTTGCGATTACTGAGGGAGTCCCCATACCCACGATCTGAGCTTGAACCGGACACACTCGAGAATATTGTGTCCAACATTATCTCTCAGATTGATGGTGAGGATCAGTCAGGCAAAGCAAAGAAGATGCTAGCTGAGATGGTTCAAGTTAGCATGGAACAGAGCTTGAGACATTTACAGCAAAGGGCTCTGGTATGCACACCAACTGATCTCCCTATCAATAGTTGTACCTCTGAGGTTTCTTTAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm

Protein Analysis

385

Amino Acids

41.18

Weight (kDa)

5.67

Isoelectric Point (pI)

64.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_ARMC5 PF24768 31 - 167 3.2e-09 ARMC5
ARM_PUB PF25598 48 - 309 8.3e-15 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 174 - 346 9.2e-08 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012800)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03440
fragaria_vesca FvH4_1g29130
malus_domestica MD09G1261900.v1.1 MD17G1256900.v1.1
prunus_persica Prupe.3G159600_v2.0.a1
pyrus_communis pycom09g17620 pycom17g25920
rosa_chinensis RchiOBHm_Chr3g0493501 RchiOBHm_Chr3g0493511
rosa_laevigata RLG00000022710
rosa_multiflora Rmu_co8033802.1_g000001
rosa_roxburghii Rroxscaffold_6G00391540
rosa_rugosa Rorug03G0261600
rosa_samantha Rh3AG310500 Rh3BG346700 Rh3CG344500 Rh3DG345800
rosa_wichuraiana Rw3G027290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 617
AccB1I GGYRCC 3 cut(s) 140, 186, 452
AciI CCGC 3 cut(s) 66, 261, 450
AclWI GGATC 2 cut(s) 751, 1014
AcsI RAATTY 2 cut(s) 150, 367
AcuI CTGAAG 1 cut(s) 341
AcyI GRCGYC 2 cut(s) 157, 187
AfaI GTAC 2 cut(s) 58, 1132
AfiI CCNNNNNNNGG 1 cut(s) 177
AgsI TTSAA 9 cut(s) 130, 473, 569, 624, 737, 752, 869, 950, 1049
AjnI CCWGG 3 cut(s) 135, 144, 844
AluBI AGCT 8 cut(s) 133, 326, 416, 632, 859, 946, 1037, 1068
AluI AGCT 8 cut(s) 133, 326, 416, 632, 859, 946, 1037, 1068
Alw21I GWGCWC 3 cut(s) 649, 778, 861
Alw26I GTCTC 3 cut(s) 223, 888, 1066
AlwI GGATC 2 cut(s) 751, 1014
AlwNI CAGNNNCTG 1 cut(s) 419
Ama87I CYCGRG 1 cut(s) 962
AoxI GGCC 1 cut(s) 208
ApaI GGGCCC 1 cut(s) 212
ApoI RAATTY 2 cut(s) 150, 367
Asp700I GAANNNNTTC 1 cut(s) 822
AspLEI GCGC 1 cut(s) 189
AspS9I GGNCC 2 cut(s) 208, 209
AsuHPI GGTGA 4 cut(s) 154, 234, 397, 1013
AsuNHI GCTAGC 1 cut(s) 1033
AvaI CYCGRG 1 cut(s) 962
BaeGI GKGCMC 2 cut(s) 145, 212
BanI GGYRCC 3 cut(s) 140, 186, 452
BanII GRGCYC 3 cut(s) 212, 861, 1093
Bbv12I GWGCWC 3 cut(s) 649, 778, 861
BccI CCATC 4 cut(s) 88, 479, 992, 1036
BceAI ACGGC 1 cut(s) 272
BciT130I CCWGG 3 cut(s) 137, 146, 846
BcoDI GTCTC 3 cut(s) 223, 888, 1066
BfaI CTAG 2 cut(s) 401, 1034
BfmI CTRYAG 1 cut(s) 377
BfoI RGCGCY 1 cut(s) 190
BfuAI ACCTGC 1 cut(s) 617
BisI GCNGC 1 cut(s) 67
BlsI GCNGC 1 cut(s) 68
Bme1390I CCNGG 3 cut(s) 137, 146, 846
BmeT110I CYCGRG 1 cut(s) 962
BmgT120I GGNCC 2 cut(s) 208, 209
BmiI GGNNCC 4 cut(s) 142, 188, 210, 454
BmrFI CCNGG 3 cut(s) 137, 146, 846
BmsI GCATC 4 cut(s) 404, 567, 794, 1020
BmtI GCTAGC 1 cut(s) 1037
BoxI GACNNNNGTC 1 cut(s) 45
BpmI CTGGAG 1 cut(s) 119
Bpu10I CCTNAGC 1 cut(s) 554
BpuEI CTTGAG 2 cut(s) 875, 1090
BsaHI GRCGYC 2 cut(s) 157, 187
BsaJI CCNNGG 3 cut(s) 136, 144, 844
BsaWI WCCGGW 1 cut(s) 952
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bsc4I CCNNNNNNNGG 1 cut(s) 177
Bse1I ACTGG 2 cut(s) 434, 578
BseBI CCWGG 3 cut(s) 137, 146, 846
BseDI CCNNGG 3 cut(s) 136, 144, 844
BseGI GGATG 4 cut(s) 35, 80, 301, 695
BseLI CCNNNNNNNGG 1 cut(s) 177
BseMII CTCAG 7 cut(s) 188, 899, 908, 933, 1004, 1029, 1128
BseNI ACTGG 2 cut(s) 434, 578
BseRI GAGGAG 5 cut(s) 4, 7, 10, 257, 266
BseSI GKGCMC 2 cut(s) 145, 212
BshFI GGCC 1 cut(s) 210
BshNI GGYRCC 3 cut(s) 140, 186, 452
BsiHKAI GWGCWC 3 cut(s) 649, 778, 861
BsiHKCI CYCGRG 1 cut(s) 962
BsiSI CCGG 1 cut(s) 953
BslFI GGGAC 2 cut(s) 566, 910
BslI CCNNNNNNNGG 1 cut(s) 177
BsmAI GTCTC 3 cut(s) 223, 888, 1066
BsmBI CGTCTC 1 cut(s) 223
BsmFI GGGAC 2 cut(s) 566, 910
BsnI GGCC 1 cut(s) 210
BsoBI CYCGRG 1 cut(s) 962
Bsp120I GGGCCC 1 cut(s) 208
Bsp1286I GDGCHC 6 cut(s) 145, 212, 649, 778, 861, 1093
Bsp143I GATC 4 cut(s) 743, 938, 1006, 1111
BspACI CCGC 3 cut(s) 66, 261, 450
BspANI GGCC 1 cut(s) 210
BspCNI CTCAG 7 cut(s) 187, 898, 909, 934, 1003, 1030, 1129
BspHI TCATGA 1 cut(s) 253
BspLI GGNNCC 4 cut(s) 142, 188, 210, 454
BspMAI CTGCAG 1 cut(s) 381
BspMI ACCTGC 1 cut(s) 617
BspOI GCTAGC 1 cut(s) 1037
BspPI GGATC 2 cut(s) 751, 1014
BspT107I GGYRCC 3 cut(s) 140, 186, 452
BsrI ACTGG 2 cut(s) 434, 578
BssECI CCNNGG 3 cut(s) 136, 144, 844
BssMI GATC 4 cut(s) 743, 938, 1006, 1111
BssNI GRCGYC 2 cut(s) 157, 187
Bst2UI CCWGG 3 cut(s) 137, 146, 846
Bst4CI ACNGT 2 cut(s) 44, 865
Bst6I CTCTTC 1 cut(s) 468
BstACI GRCGYC 2 cut(s) 157, 187
BstC8I GCNNGC 5 cut(s) 71, 448, 717, 765, 1035
BstDEI CTNAG 9 cut(s) 174, 479, 554, 885, 917, 942, 990, 1038, 1137
BstENI CCTNNNNNAGG 1 cut(s) 175
BstF5I GGATG 4 cut(s) 35, 80, 301, 695
BstH2I RGCGCY 1 cut(s) 190
BstHHI GCGC 1 cut(s) 189
BstKTI GATC 4 cut(s) 746, 941, 1009, 1114
BstMAI GTCTC 3 cut(s) 223, 888, 1066
BstMBI GATC 4 cut(s) 743, 938, 1006, 1111
BstMWI GCNNNNNNNGC 3 cut(s) 269, 452, 773
BstNI CCWGG 3 cut(s) 137, 146, 846
BstNSI RCATGY 1 cut(s) 767
BstPAI GACNNNNGTC 1 cut(s) 45
BstSCI CCNGG 3 cut(s) 135, 144, 844
BstSFI CTRYAG 1 cut(s) 377
BstSLI GKGCMC 2 cut(s) 145, 212
BsuRI GGCC 1 cut(s) 210
BtsCI GGATG 4 cut(s) 35, 80, 301, 695
BtsIMutI CAGTG 1 cut(s) 861
BveI ACCTGC 1 cut(s) 617
Cac8I GCNNGC 5 cut(s) 71, 448, 717, 765, 1035
CaiI CAGNNNCTG 1 cut(s) 419
CciI TCATGA 1 cut(s) 253
CfoI GCGC 1 cut(s) 189
Cfr13I GGNCC 2 cut(s) 208, 209
CseI GACGC 1 cut(s) 165
Csp6I GTAC 2 cut(s) 57, 1131
CviAII CATG 4 cut(s) 223, 254, 764, 1057
CviQI GTAC 2 cut(s) 57, 1131
DdeI CTNAG 9 cut(s) 174, 479, 554, 885, 917, 942, 990, 1038, 1137
DinI GGCGCC 1 cut(s) 188
DpnI GATC 4 cut(s) 745, 940, 1008, 1113
DpnII GATC 4 cut(s) 743, 938, 1006, 1111
DraI TTTAAA 1 cut(s) 1149
Eam1104I CTCTTC 1 cut(s) 468
EarI CTCTTC 1 cut(s) 468
EciI GGCGGA 1 cut(s) 250
Ecl136II GAGCTC 1 cut(s) 859
Eco24I GRGCYC 3 cut(s) 212, 861, 1093
Eco53kI GAGCTC 1 cut(s) 859
Eco57I CTGAAG 1 cut(s) 341
Eco88I CYCGRG 1 cut(s) 962
EcoICRI GAGCTC 1 cut(s) 859
EcoNI CCTNNNNNAGG 1 cut(s) 175
EcoRII CCWGG 3 cut(s) 135, 144, 844
EcoT38I GRGCYC 3 cut(s) 212, 861, 1093
EgeI GGCGCC 1 cut(s) 188
EheI GGCGCC 1 cut(s) 188
Esp3I CGTCTC 1 cut(s) 223
FaeI CATG 4 cut(s) 226, 257, 767, 1060
FalI AAGNNNNNCTT 2 cut(s) 810, 842
FaqI GGGAC 2 cut(s) 566, 910
FatI CATG 4 cut(s) 222, 253, 763, 1056
Fnu4HI GCNGC 1 cut(s) 67
FokI GGATG 4 cut(s) 22, 67, 308, 682
FriOI GRGCYC 3 cut(s) 212, 861, 1093
Fsp4HI GCNGC 1 cut(s) 67
FspBI CTAG 2 cut(s) 401, 1034
GlaI GCGC 1 cut(s) 188
GluI GCNGC 1 cut(s) 67
GsuI CTGGAG 1 cut(s) 119
HaeII RGCGCY 1 cut(s) 190
HaeIII GGCC 1 cut(s) 210
HapII CCGG 1 cut(s) 953
HgaI GACGC 1 cut(s) 165
HhaI GCGC 1 cut(s) 189
Hin1I GRCGYC 2 cut(s) 157, 187
Hin1II CATG 4 cut(s) 226, 257, 767, 1060
Hin6I GCGC 1 cut(s) 187
HinP1I GCGC 1 cut(s) 187
HinfI GANTC 8 cut(s) 5, 200, 239, 257, 509, 569, 656, 923
HpaII CCGG 1 cut(s) 953
HphI GGTGA 4 cut(s) 154, 234, 397, 1013
Hpy166II GTNNAC 1 cut(s) 235
Hpy188III TCNNGA 6 cut(s) 236, 254, 566, 758, 854, 964
Hpy8I GTNNAC 1 cut(s) 235
Hpy99I CGWCG 3 cut(s) 44, 93, 159
HpyAV CCTTC 3 cut(s) 365, 698, 827
HpyCH4III ACNGT 2 cut(s) 44, 865
HpyCH4V TGCA 7 cut(s) 379, 389, 395, 612, 719, 807, 1101
HpyF10VI GCNNNNNNNGC 3 cut(s) 269, 452, 773
HpyF3I CTNAG 9 cut(s) 174, 479, 554, 885, 917, 942, 990, 1038, 1137
Hsp92I GRCGYC 2 cut(s) 157, 187
Hsp92II CATG 4 cut(s) 226, 257, 767, 1060
HspAI GCGC 1 cut(s) 187
KasI GGCGCC 1 cut(s) 186
Kzo9I GATC 4 cut(s) 743, 938, 1006, 1111
LmnI GCTCC 2 cut(s) 138, 231
LweI GCATC 4 cut(s) 404, 567, 794, 1020
MaeI CTAG 2 cut(s) 401, 1034
MaeIII GTNAC 3 cut(s) 403, 592, 797
MalI GATC 4 cut(s) 745, 940, 1008, 1113
MboI GATC 4 cut(s) 743, 938, 1006, 1111
MboII GAAGA 4 cut(s) 455, 612, 713, 1039
MhlI GDGCHC 6 cut(s) 145, 212, 649, 778, 861, 1093
MluCI AATT 4 cut(s) 150, 345, 367, 546
Mly113I GGCGCC 1 cut(s) 187
MlyI GAGTC 4 cut(s) 194, 233, 266, 932
MmeI TCCRAC 6 cut(s) 29, 63, 78, 222, 450, 1002
MroXI GAANNNNTTC 1 cut(s) 822
MseI TTAA 4 cut(s) 549, 602, 828, 1148
MspA1I CMGCKG 1 cut(s) 632
MspI CCGG 1 cut(s) 953
MspR9I CCNGG 3 cut(s) 137, 146, 846
MvaI CCWGG 3 cut(s) 137, 146, 846
MwoI GCNNNNNNNGC 3 cut(s) 269, 452, 773
NarI GGCGCC 1 cut(s) 187
NdeII GATC 4 cut(s) 743, 938, 1006, 1111
NheI GCTAGC 1 cut(s) 1033
NlaIII CATG 4 cut(s) 226, 257, 767, 1060
NlaIV GGNNCC 4 cut(s) 142, 188, 210, 454
NmuCI GTSAC 2 cut(s) 403, 797
NspI RCATGY 1 cut(s) 767
PaeI GCATGC 1 cut(s) 767
PaeR7I CTCGAG 1 cut(s) 962
PagI TCATGA 1 cut(s) 253
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PdmI GAANNNNTTC 1 cut(s) 822
PfeI GAWTC 4 cut(s) 5, 509, 569, 656
PkrI GCNGC 1 cut(s) 68
PleI GAGTC 4 cut(s) 194, 233, 265, 931
PluTI GGCGCC 1 cut(s) 190
PpsI GAGTC 4 cut(s) 194, 233, 265, 931
PshAI GACNNNNGTC 1 cut(s) 45
Psp124BI GAGCTC 1 cut(s) 861
Psp6I CCWGG 3 cut(s) 135, 144, 844
PspGI CCWGG 3 cut(s) 135, 144, 844
PspN4I GGNNCC 4 cut(s) 142, 188, 210, 454
PspOMI GGGCCC 1 cut(s) 208
PspPI GGNCC 2 cut(s) 208, 209
PstI CTGCAG 1 cut(s) 381
PstNI CAGNNNCTG 1 cut(s) 419
PvuII CAGCTG 1 cut(s) 632
RsaI GTAC 2 cut(s) 58, 1132
RsaNI GTAC 2 cut(s) 57, 1131
SacI GAGCTC 1 cut(s) 861
SaqAI TTAA 4 cut(s) 549, 602, 828, 1148
SatI GCNGC 1 cut(s) 67
Sau3AI GATC 4 cut(s) 743, 938, 1006, 1111
Sau96I GGNCC 2 cut(s) 208, 209
SchI GAGTC 4 cut(s) 194, 233, 266, 932
ScrFI CCNGG 3 cut(s) 137, 146, 846
SduI GDGCHC 6 cut(s) 145, 212, 649, 778, 861, 1093
SfaNI GCATC 4 cut(s) 404, 567, 794, 1020
SfcI CTRYAG 1 cut(s) 377
SfoI GGCGCC 1 cut(s) 188
Sfr274I CTCGAG 1 cut(s) 962
SlaI CTCGAG 1 cut(s) 962
SmlI CTYRAG 3 cut(s) 854, 962, 1069
SmoI CTYRAG 3 cut(s) 854, 962, 1069
SphI GCATGC 1 cut(s) 767
Sse9I AATT 4 cut(s) 150, 345, 367, 546
SsiI CCGC 3 cut(s) 66, 261, 450
SspDI GGCGCC 1 cut(s) 186
SspI AATATT 1 cut(s) 970
SspMI CTAG 2 cut(s) 401, 1034
SstI GAGCTC 1 cut(s) 861
StyD4I CCNGG 3 cut(s) 135, 144, 844
TaaI ACNGT 2 cut(s) 44, 865
TaqI TCGA 4 cut(s) 154, 572, 757, 963
TasI AATT 4 cut(s) 150, 345, 367, 546
TauI GCSGC 1 cut(s) 69
TfiI GAWTC 4 cut(s) 5, 509, 569, 656
Tru1I TTAA 4 cut(s) 549, 602, 828, 1148
Tru9I TTAA 4 cut(s) 549, 602, 828, 1148
TscAI CASTG 1 cut(s) 868
TseFI GTSAC 2 cut(s) 403, 797
Tsp45I GTSAC 2 cut(s) 403, 797
TspDTI ATGAA 1 cut(s) 242
TspGWI ACGGA 2 cut(s) 73, 77
TspRI CASTG 1 cut(s) 868
XagI CCTNNNNNAGG 1 cut(s) 175
XapI RAATTY 2 cut(s) 150, 367
XceI RCATGY 1 cut(s) 767
XhoI CTCGAG 1 cut(s) 962
XmnI GAANNNNTTC 1 cut(s) 822
XspI CTAG 2 cut(s) 401, 1034
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.