pycom17g25920

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0012800
Physical Location & Seq
Reverse (-)
24259076 .. 24262435
3360 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g25920.1

Sequence Viewer

Length: 1149 bp
ATGGATTCCCGCTCATCATCCGACGGTCGGAGTTCGACTCCGGACTCACTCTCTCCCACCTTCTCCTCATCCTCAGCCGTCCATCGCGCTCTGGAACTCATCCAATCCGACGACCAGGATTCCAAGCTCCAGGGCGCCCAGGAAATCCGGCGCCTCACCAAGACCTCGCAGCGGTGTCGGCGCCAGCTCTCCGACTCGGTGGGCCCACTCGTGTCCATGCTCCGCCTTCGTGACTCGCCCGAGTCCCAAGAGTCGGCCCTACTCGCCCTCCTCAACCTCGCCGTCAAGGATGAGACGAACAAAATTAAGATAGTGGAAGCTGGCGCTTTAGAGCCAATAATTGCTTTCCTTCAGTCACAAAGTTCCACCCTGCAGGAGTATGCAACTGCAGCTCTACTCACTCTATCAGCTTCTGCCGTCAACAAGCCAGTTATTGGTGCTTCCGGTGCCATTCCTCTTCTCGTCGAAATCCTTAGACATGGCAGCCCACAAGCTAAAGTTGACTCAGTAATGGCGCTTTCCAATCTCTCAACACACCCTTCTAATCTTAGCATCATTCTTGAAGCAAAACCAATTCCGTCCATAGTTACTTTGTTAAAGCCCTGCAAGAAATCTTCAAAAACAGCTGAGAGATGCTGTGCTCTGATAGAATCTCTCGTGGGATTCGACGAGGGAAGAACTGCCCTCACGTCTGAAGAAGGCGGAGTGCTTGCAGTGATTGAGGTGCTTGAAAATGGATCGCTCCAAAGTAGGGAGCACGCTGTAAGTACTCTACTAACAATGTGTCAAAGTGACAGGTGCAGATATAGAGAAGCGATTCTTAAAGACGGGGTAATCCCCGGACTTCTCGAGCTCACAGTTCAAGGAACGCCTAAGTCTCAGACAAAAGCGCATACGGTTTTGCGCTTGTTGAGGGAGTCTCCATACCCGAGATCAGAGCTTGAAGCAGACACTCTGGAGAACATTGTATGCAACATCATCTCTCAAATTGACAGTGATGATCAGTCCAGTAAAGCGAAGAAAATTCTGGCCGATATGGTTCAAGTTAGCATGGAGCAAAGCTTGAGACATTTACAGCAAAGGGCTCTGGTATGCACATCGACTGATCTGCCTATCAGTACTTGCACCTCTGAAGTATCTTTGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm

Protein Analysis

383

Amino Acids

41.23

Weight (kDa)

5.96

Isoelectric Point (pI)

63.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_ARMC5 PF24768 25 - 164 1.8e-09 ARMC5
ARM_PUB PF25598 40 - 307 1.5e-14 Plant U-box protein ARM repeat region
Arm PF00514 100 - 136 5e-06 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 172 - 349 1.4e-08 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012800)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03440
fragaria_vesca FvH4_1g29130
malus_domestica MD09G1261900.v1.1 MD17G1256900.v1.1
prunus_persica Prupe.3G159600_v2.0.a1
pyrus_communis pycom09g17620 pycom17g25920
rosa_chinensis RchiOBHm_Chr3g0493501 RchiOBHm_Chr3g0493511
rosa_laevigata RLG00000022710
rosa_multiflora Rmu_co8033802.1_g000001
rosa_roxburghii Rroxscaffold_6G00391540
rosa_rugosa Rorug03G0261600
rosa_samantha Rh3AG310500 Rh3BG346700 Rh3CG344500 Rh3DG345800
rosa_wichuraiana Rw3G027290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 134, 150, 180, 446
AccB7I CCANNNNNTGG 1 cut(s) 434
AccBSI CCGCTC 1 cut(s) 12
AccII CGCG 1 cut(s) 87
AccIII TCCGGA 1 cut(s) 40
AciI CCGC 4 cut(s) 10, 172, 223, 702
AclWI GGATC 1 cut(s) 745
AcoI YGGCCR 1 cut(s) 1029
AcsI RAATTY 1 cut(s) 1023
AcuI CTGAAG 2 cut(s) 335, 714
AcyI GRCGYC 3 cut(s) 135, 151, 181
AfaI GTAC 2 cut(s) 769, 1120
AfiI CCNNNNNNNGG 5 cut(s) 27, 171, 253, 434, 751
AgsI TTSAA 7 cut(s) 563, 618, 731, 863, 944, 1043, 1144
AjiI CACGTC 1 cut(s) 690
AjnI CCWGG 3 cut(s) 114, 129, 138
Alw21I GWGCWC 3 cut(s) 643, 759, 855
Alw26I GTCTC 4 cut(s) 287, 882, 924, 1060
AlwI GGATC 1 cut(s) 745
AlwNI CAGNNNCTG 1 cut(s) 413
Ama87I CYCGRG 3 cut(s) 239, 848, 928
Aor13HI TCCGGA 1 cut(s) 40
AoxI GGCC 3 cut(s) 202, 255, 1029
ApaI GGGCCC 1 cut(s) 206
ApeKI GCWGC 3 cut(s) 169, 389, 483
ApoI RAATTY 1 cut(s) 1023
Asp700I GAANNNNTTC 1 cut(s) 816
AspLEI GCGC 8 cut(s) 89, 137, 153, 183, 326, 517, 892, 906
AspS9I GGNCC 3 cut(s) 202, 203, 256
AsuC2I CCSGG 1 cut(s) 840
AsuHPI GGTGA 1 cut(s) 148
AvaI CYCGRG 3 cut(s) 239, 848, 928
BaeGI GKGCMC 1 cut(s) 206
BanI GGYRCC 4 cut(s) 134, 150, 180, 446
BanII GRGCYC 3 cut(s) 206, 855, 1087
BauI CACGAG 2 cut(s) 209, 656
Bbv12I GWGCWC 3 cut(s) 643, 759, 855
BbvCI CCTCAGC 1 cut(s) 73
BbvI GCAGC 3 cut(s) 181, 401, 495
BccI CCATC 1 cut(s) 90
BceAI ACGGC 3 cut(s) 62, 266, 401
BcgI CGANNNNNNTGC 4 cut(s) 158, 192, 1090, 1124
BciT130I CCWGG 3 cut(s) 116, 131, 140
BclI TGATCA 1 cut(s) 1000
BcnI CCSGG 1 cut(s) 840
BcoDI GTCTC 4 cut(s) 287, 882, 924, 1060
BfmI CTRYAG 2 cut(s) 371, 387
BfoI RGCGCY 5 cut(s) 138, 154, 184, 327, 518
BisI GCNGC 3 cut(s) 170, 390, 484
BlsI GCNGC 3 cut(s) 171, 391, 485
BmcAI AGTACT 2 cut(s) 769, 1120
Bme1390I CCNGG 4 cut(s) 116, 131, 140, 840
BmeT110I CYCGRG 3 cut(s) 239, 848, 928
BmgBI CACGTC 1 cut(s) 690
BmgT120I GGNCC 3 cut(s) 202, 203, 256
BmiI GGNNCC 5 cut(s) 136, 152, 182, 204, 448
BmrFI CCNGG 4 cut(s) 116, 131, 140, 840
BmsI GCATC 2 cut(s) 561, 623
BplI GAGNNNNNCTC 4 cut(s) 22, 54, 904, 936
BpmI CTGGAG 2 cut(s) 113, 977
Bpu10I CCTNAGC 1 cut(s) 73
BpuEI CTTGAG 1 cut(s) 1084
BpuMI CCSGG 1 cut(s) 840
BsaHI GRCGYC 3 cut(s) 135, 151, 181
BsaJI CCNNGG 3 cut(s) 130, 138, 838
BsaWI WCCGGW 2 cut(s) 40, 443
BsaXI ACNNNNNCTCC 2 cut(s) 252, 282
Bsc4I CCNNNNNNNGG 5 cut(s) 27, 171, 253, 434, 751
Bse1I ACTGG 2 cut(s) 428, 1008
BseAI TCCGGA 1 cut(s) 40
BseBI CCWGG 3 cut(s) 116, 131, 140
BseDI CCNNGG 3 cut(s) 130, 138, 838
BseGI GGATG 4 cut(s) 17, 68, 99, 295
BseLI CCNNNNNNNGG 5 cut(s) 27, 171, 253, 434, 751
BseMII CTCAG 4 cut(s) 87, 519, 618, 893
BseNI ACTGG 2 cut(s) 428, 1008
BseRI GAGGAG 2 cut(s) 55, 260
BseSI GKGCMC 1 cut(s) 206
BseXI GCAGC 3 cut(s) 181, 401, 495
BsgI GTGCAG 1 cut(s) 820
Bsh1236I CGCG 1 cut(s) 87
Bsh1285I CGRYCG 1 cut(s) 28
BshFI GGCC 3 cut(s) 204, 257, 1031
BshNI GGYRCC 4 cut(s) 134, 150, 180, 446
BsiEI CGRYCG 1 cut(s) 28
BsiHKAI GWGCWC 3 cut(s) 643, 759, 855
BsiHKCI CYCGRG 3 cut(s) 239, 848, 928
BsiSI CCGG 4 cut(s) 41, 148, 444, 840
BslFI GGGAC 1 cut(s) 229
BslI CCNNNNNNNGG 5 cut(s) 27, 171, 253, 434, 751
BsmAI GTCTC 4 cut(s) 287, 882, 924, 1060
BsmBI CGTCTC 1 cut(s) 287
BsmFI GGGAC 1 cut(s) 229
BsnI GGCC 3 cut(s) 204, 257, 1031
BsoBI CYCGRG 3 cut(s) 239, 848, 928
Bsp120I GGGCCC 1 cut(s) 202
Bsp1286I GDGCHC 5 cut(s) 206, 643, 759, 855, 1087
Bsp13I TCCGGA 1 cut(s) 40
Bsp143I GATC 4 cut(s) 737, 932, 1000, 1105
BspACI CCGC 4 cut(s) 10, 172, 223, 702
BspANI GGCC 3 cut(s) 204, 257, 1031
BspCNI CTCAG 4 cut(s) 86, 518, 619, 892
BspEI TCCGGA 1 cut(s) 40
BspFNI CGCG 1 cut(s) 87
BspLI GGNNCC 5 cut(s) 136, 152, 182, 204, 448
BspMAI CTGCAG 2 cut(s) 375, 391
BspPI GGATC 1 cut(s) 745
BspT107I GGYRCC 4 cut(s) 134, 150, 180, 446
BsrBI CCGCTC 1 cut(s) 12
BsrI ACTGG 2 cut(s) 428, 1008
BssECI CCNNGG 3 cut(s) 130, 138, 838
BssMI GATC 4 cut(s) 737, 932, 1000, 1105
BssNI GRCGYC 3 cut(s) 135, 151, 181
BssSI CACGAG 2 cut(s) 209, 656
Bst2BI CACGAG 2 cut(s) 209, 656
Bst2UI CCWGG 3 cut(s) 116, 131, 140
Bst4CI ACNGT 4 cut(s) 26, 859, 898, 995
Bst6I CTCTTC 1 cut(s) 462
BstACI GRCGYC 3 cut(s) 135, 151, 181
BstC8I GCNNGC 4 cut(s) 185, 322, 711, 759
BstDEI CTNAG 7 cut(s) 73, 473, 505, 548, 627, 873, 879
BstF5I GGATG 4 cut(s) 17, 68, 99, 295
BstFNI CGCG 1 cut(s) 87
BstH2I RGCGCY 5 cut(s) 138, 154, 184, 327, 518
BstHHI GCGC 8 cut(s) 89, 137, 153, 183, 326, 517, 892, 906
BstKTI GATC 4 cut(s) 740, 935, 1003, 1108
BstMAI GTCTC 4 cut(s) 287, 882, 924, 1060
BstMBI GATC 4 cut(s) 737, 932, 1000, 1105
BstMCI CGRYCG 1 cut(s) 28
BstMWI GCNNNNNNNGC 4 cut(s) 178, 263, 389, 446
BstNI CCWGG 3 cut(s) 116, 131, 140
BstSCI CCNGG 4 cut(s) 114, 129, 138, 838
BstSFI CTRYAG 2 cut(s) 371, 387
BstSLI GKGCMC 1 cut(s) 206
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 3 cut(s) 181, 401, 495
BsuRI GGCC 3 cut(s) 204, 257, 1031
BtgZI GCGATG 1 cut(s) 68
BtrI CACGTC 1 cut(s) 690
BtsCI GGATG 4 cut(s) 17, 68, 99, 295
BtsI GCAGTG 1 cut(s) 720
BtsIMutI CAGTG 2 cut(s) 720, 1000
Cac8I GCNNGC 4 cut(s) 185, 322, 711, 759
CaiI CAGNNNCTG 1 cut(s) 413
CfoI GCGC 8 cut(s) 89, 137, 153, 183, 326, 517, 892, 906
Cfr13I GGNCC 3 cut(s) 202, 203, 256
Csp6I GTAC 2 cut(s) 768, 1119
CviAII CATG 3 cut(s) 217, 479, 1051
CviQI GTAC 2 cut(s) 768, 1119
DdeI CTNAG 7 cut(s) 73, 473, 505, 548, 627, 873, 879
DinI GGCGCC 3 cut(s) 136, 152, 182
DpnI GATC 4 cut(s) 739, 934, 1002, 1107
DpnII GATC 4 cut(s) 737, 932, 1000, 1105
EaeI YGGCCR 1 cut(s) 1029
Eam1104I CTCTTC 1 cut(s) 462
EarI CTCTTC 1 cut(s) 462
EciI GGCGGA 2 cut(s) 212, 717
Ecl136II GAGCTC 1 cut(s) 853
Eco24I GRGCYC 3 cut(s) 206, 855, 1087
Eco53kI GAGCTC 1 cut(s) 853
Eco57I CTGAAG 2 cut(s) 335, 714
Eco88I CYCGRG 3 cut(s) 239, 848, 928
EcoICRI GAGCTC 1 cut(s) 853
EcoRII CCWGG 3 cut(s) 114, 129, 138
EcoT38I GRGCYC 3 cut(s) 206, 855, 1087
EgeI GGCGCC 3 cut(s) 136, 152, 182
EheI GGCGCC 3 cut(s) 136, 152, 182
Esp3I CGTCTC 1 cut(s) 287
FaeI CATG 3 cut(s) 220, 482, 1054
FalI AAGNNNNNCTT 2 cut(s) 804, 836
FaqI GGGAC 1 cut(s) 229
FatI CATG 3 cut(s) 216, 478, 1050
FauI CCCGC 1 cut(s) 17
FbaI TGATCA 1 cut(s) 1000
Fnu4HI GCNGC 3 cut(s) 170, 390, 484
FokI GGATG 4 cut(s) 4, 55, 86, 302
FriOI GRGCYC 3 cut(s) 206, 855, 1087
Fsp4HI GCNGC 3 cut(s) 170, 390, 484
GlaI GCGC 8 cut(s) 88, 136, 152, 182, 325, 516, 891, 905
GluI GCNGC 3 cut(s) 170, 390, 484
GsuI CTGGAG 2 cut(s) 113, 977
HaeII RGCGCY 5 cut(s) 138, 154, 184, 327, 518
HaeIII GGCC 3 cut(s) 204, 257, 1031
HapII CCGG 4 cut(s) 41, 148, 444, 840
HhaI GCGC 8 cut(s) 89, 137, 153, 183, 326, 517, 892, 906
Hin1I GRCGYC 3 cut(s) 135, 151, 181
Hin1II CATG 3 cut(s) 220, 482, 1054
Hin6I GCGC 8 cut(s) 87, 135, 151, 181, 324, 515, 890, 904
HinP1I GCGC 8 cut(s) 87, 135, 151, 181, 324, 515, 890, 904
HincII GTYRAC 2 cut(s) 421, 502
HindII GTYRAC 2 cut(s) 421, 502
HindIII AAGCTT 1 cut(s) 1060
HpaII CCGG 4 cut(s) 41, 148, 444, 840
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 2 cut(s) 421, 502
Hpy188I TCNGA 9 cut(s) 22, 30, 109, 193, 645, 694, 882, 937, 1132
Hpy188III TCNNGA 6 cut(s) 41, 92, 230, 560, 848, 956
Hpy8I GTNNAC 2 cut(s) 421, 502
Hpy99I CGWCG 4 cut(s) 26, 113, 467, 671
HpyAV CCTTC 5 cut(s) 70, 236, 359, 549, 692
HpyCH4III ACNGT 4 cut(s) 26, 859, 898, 995
HpyCH4IV ACGT 1 cut(s) 689
HpyCH4V TGCA 9 cut(s) 373, 383, 389, 606, 713, 801, 972, 1095, 1125
HpyF10VI GCNNNNNNNGC 4 cut(s) 178, 263, 389, 446
HpyF3I CTNAG 7 cut(s) 73, 473, 505, 548, 627, 873, 879
HpySE526I ACGT 1 cut(s) 689
Hsp92I GRCGYC 3 cut(s) 135, 151, 181
Hsp92II CATG 3 cut(s) 220, 482, 1054
HspAI GCGC 8 cut(s) 87, 135, 151, 181, 324, 515, 890, 904
KasI GGCGCC 3 cut(s) 134, 150, 180
Kpn2I TCCGGA 1 cut(s) 40
Ksp22I TGATCA 1 cut(s) 1000
Kzo9I GATC 4 cut(s) 737, 932, 1000, 1105
LmnI GCTCC 5 cut(s) 132, 225, 747, 754, 1054
Lsp1109I GCAGC 3 cut(s) 181, 401, 495
LweI GCATC 2 cut(s) 561, 623
MaeII ACGT 1 cut(s) 689
MaeIII GTNAC 4 cut(s) 230, 354, 586, 791
MalI GATC 4 cut(s) 739, 934, 1002, 1107
MbiI CCGCTC 1 cut(s) 12
MboI GATC 4 cut(s) 737, 932, 1000, 1105
MboII GAAGA 5 cut(s) 449, 606, 687, 707, 1030
MhlI GDGCHC 5 cut(s) 206, 643, 759, 855, 1087
MluCI AATT 5 cut(s) 303, 339, 573, 987, 1023
Mly113I GGCGCC 3 cut(s) 135, 151, 181
MlyI GAGTC 8 cut(s) 31, 38, 188, 227, 251, 260, 497, 926
MmeI TCCRAC 4 cut(s) 8, 45, 132, 216
MroI TCCGGA 1 cut(s) 40
MroXI GAANNNNTTC 1 cut(s) 816
MseI TTAA 3 cut(s) 306, 596, 822
MspA1I CMGCKG 2 cut(s) 172, 626
MspI CCGG 4 cut(s) 41, 148, 444, 840
MspR9I CCNGG 4 cut(s) 116, 131, 140, 840
MvaI CCWGG 3 cut(s) 116, 131, 140
MvnI CGCG 1 cut(s) 87
MwoI GCNNNNNNNGC 4 cut(s) 178, 263, 389, 446
NarI GGCGCC 3 cut(s) 135, 151, 181
NciI CCSGG 1 cut(s) 840
NdeII GATC 4 cut(s) 737, 932, 1000, 1105
NlaIII CATG 3 cut(s) 220, 482, 1054
NlaIV GGNNCC 5 cut(s) 136, 152, 182, 204, 448
NmuCI GTSAC 3 cut(s) 230, 354, 791
PaeR7I CTCGAG 1 cut(s) 848
PcsI WCGNNNNNNNCGW 1 cut(s) 663
PdmI GAANNNNTTC 1 cut(s) 816
PfeI GAWTC 5 cut(s) 5, 119, 650, 663, 817
PflMI CCANNNNNTGG 1 cut(s) 434
PkrI GCNGC 3 cut(s) 171, 391, 485
PleI GAGTC 8 cut(s) 31, 38, 188, 227, 250, 259, 497, 925
PluTI GGCGCC 3 cut(s) 138, 154, 184
PpsI GAGTC 8 cut(s) 31, 38, 188, 227, 250, 259, 497, 925
Psp124BI GAGCTC 1 cut(s) 855
Psp6I CCWGG 3 cut(s) 114, 129, 138
PspGI CCWGG 3 cut(s) 114, 129, 138
PspN4I GGNNCC 5 cut(s) 136, 152, 182, 204, 448
PspOMI GGGCCC 1 cut(s) 202
PspPI GGNCC 3 cut(s) 202, 203, 256
PstI CTGCAG 2 cut(s) 375, 391
PstNI CAGNNNCTG 1 cut(s) 413
PvuII CAGCTG 1 cut(s) 626
RsaI GTAC 2 cut(s) 769, 1120
RsaNI GTAC 2 cut(s) 768, 1119
SacI GAGCTC 1 cut(s) 855
SaqAI TTAA 3 cut(s) 306, 596, 822
SatI GCNGC 3 cut(s) 170, 390, 484
Sau3AI GATC 4 cut(s) 737, 932, 1000, 1105
Sau96I GGNCC 3 cut(s) 202, 203, 256
SbfI CCTGCAGG 1 cut(s) 375
ScaI AGTACT 2 cut(s) 769, 1120
SchI GAGTC 8 cut(s) 31, 38, 188, 227, 251, 260, 497, 926
ScrFI CCNGG 4 cut(s) 116, 131, 140, 840
SdaI CCTGCAGG 1 cut(s) 375
SduI GDGCHC 5 cut(s) 206, 643, 759, 855, 1087
SfaNI GCATC 2 cut(s) 561, 623
SfcI CTRYAG 2 cut(s) 371, 387
SfoI GGCGCC 3 cut(s) 136, 152, 182
Sfr274I CTCGAG 1 cut(s) 848
SlaI CTCGAG 1 cut(s) 848
SmlI CTYRAG 2 cut(s) 848, 1063
SmoI CTYRAG 2 cut(s) 848, 1063
Sse8387I CCTGCAGG 1 cut(s) 375
Sse9I AATT 5 cut(s) 303, 339, 573, 987, 1023
SsiI CCGC 4 cut(s) 10, 172, 223, 702
SspDI GGCGCC 3 cut(s) 134, 150, 180
SstI GAGCTC 1 cut(s) 855
StyD4I CCNGG 4 cut(s) 114, 129, 138, 838
TaaI ACNGT 4 cut(s) 26, 859, 898, 995
TaiI ACGT 1 cut(s) 692
TaqI TCGA 5 cut(s) 35, 465, 666, 849, 1100
TasI AATT 5 cut(s) 303, 339, 573, 987, 1023
TatI WGTACW 2 cut(s) 767, 1118
TfiI GAWTC 5 cut(s) 5, 119, 650, 663, 817
Tru1I TTAA 3 cut(s) 306, 596, 822
Tru9I TTAA 3 cut(s) 306, 596, 822
TscAI CASTG 2 cut(s) 720, 1000
TseFI GTSAC 3 cut(s) 230, 354, 791
TseI GCWGC 3 cut(s) 169, 389, 483
Tsp45I GTSAC 3 cut(s) 230, 354, 791
TspGWI ACGGA 1 cut(s) 567
TspRI CASTG 2 cut(s) 720, 1000
Van91I CCANNNNNTGG 1 cut(s) 434
XapI RAATTY 1 cut(s) 1023
XhoI CTCGAG 1 cut(s) 848
XmnI GAANNNNTTC 1 cut(s) 816
ZrmI AGTACT 2 cut(s) 769, 1120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.